id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-257456-15bm9psj	Arumugam, Arunkumar	A Rapid SARS-CoV-2 RT-PCR Assay for Low Resource Settings	2020-09-24		.txt	text/plain	5286	294	59	Using COVID-19 clinical specimens, we have collected evidence that the RT-qPCR assay can feasibly be performed directly on patient sample material in virus transport medium (VTM) without an RNA extraction step, while still producing sensitive test results. Using COVID-19 positive clinical specimens, we demonstrated that RT-PCR assays can be performed in as little as 12 min using untreated samples, heat-inactivated samples, or extracted RNA templates with our low-cost water bath setup. To further improve the speed of a diagnostic assay, we and others tested using untreated or heat-inactivated samples added directly to one-step RT-PCR master mixes without an RNA extraction step [6, [13] [14] [15] [16] [17] [18] [19] . Prior to COVID-19 emerged as a global pandemic, we have tested the feasibility of circumventing the sample preparation steps by adding a few microliters of the unprocessed sample (in VTM) directly into the RT-qPCR assay master mix targeting InfA, InfB, and RSV.	./cache/cord-257456-15bm9psj.txt	./txt/cord-257456-15bm9psj.txt