id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-255090-2gpsu1y4	Lv, Ke	Transient inhibition of foot-and-mouth disease virus replication by siRNAs silencing VP1 protein coding region	2009-06-30		.txt	text/plain	6335	318	52	In addition, variations within multiple regions of the quasispecies of FMDV were retrospectively revealed by sequencing of FMDV genes, and a single nucleotide substitution was identified as the main factor in resistance to RNAi. Our data demonstrated that the three siRNA molecules synthesized with T7 RNA polymerase could have transient inhibitory effects on the replication of FMDV. Recently, several laboratories used RNAi to inhibit foot-and-mouth virus infection in cell culture or in animals taking forms of chemically synthesized siRNA, plasmid encoding shRNA, and adenovirus encoding shRNA (Chen et al., 2004 (Chen et al., , 2006 de los Santos et al., 2005; Kahana et al., 2004; Mohapatra et al., 2005) , showing rapid decrease of FMDV replication. Furthermore, among the five siRNAs candidates, three siRNAs were confirmed efficacious to transiently inhibit the replication of FMDV in BHK-21 cells by determining the relative viral amount within infected cells and the virus titers in supernatants as well as the observation of the emergence of CPE.	./cache/cord-255090-2gpsu1y4.txt	./txt/cord-255090-2gpsu1y4.txt