id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-016179-4i1n9j4x	Chen, Yi-Ning	Real-Time Reverse Transcription-Polymerase Chain Reaction for Detection and Quantitation of Turkey Coronavirus RNA in Feces and Intestine Tissues	2015-09-10		.txt	text/plain	2078	133	62	title: Real-Time Reverse Transcription-Polymerase Chain Reaction for Detection and Quantitation of Turkey Coronavirus RNA in Feces and Intestine Tissues A specific one-step real-time reverse transcription-polymerase chain reaction (RT-PCR) assay using TCoV-specific primers and dual-labeled fluorescent probe for detection and quantitation of TCoV in feces and intestine tissues is described in this chapter. In this chapter, the protocol for one-step real-time RT-PCR to detect, differentiate, and quantitative TCoV RNA in the feces and intestinal tissue is presented. In step 3, the extracted RNA was subjected to one-step real-time RT-PCR for detection and quantitation of TCoV in feces or intestine tissues. Specific real time reverse transcription polymerase chain reaction for detection and quantitation of turkey coronavirus RNA in tissues and feces from turkeys infected with turkey coronavirus The protocol "Real-time reverse transcription-polymerase chain reaction for detection and quantitation of turkey coronavirus RNA in feces and intestine tissues" outlined in this chapter had been successfully carried out in the authors' studies on molecular diagnostics, molecular virology, immunology, and/or vaccinology of turkey coronaviral enteritis.	./cache/cord-016179-4i1n9j4x.txt	./txt/cord-016179-4i1n9j4x.txt