id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-008575-bbpmlo3c	Lawton, Jeffrey A	Mechanism of genome transcription in segmented dsRNA viruses	2004-01-07		.txt	text/plain	15879	753	43	Although no reconstitution system is yet available for any of the reoviruses, studies conducted using baculovirusexpressed recombinant rotavirus-like particles containing the viral RNA polymerase coexpressed with the inner capsid protein have begun to clarify the mechanism by which the dsRNA genome is synthesized from the mRNA templates in rotavirus Patton et al., 1996; Zeng et al., 1996) . One of the more interesting observations about the life cycle of dsRNA viruses is that genome transcription occurs only within structurally intact TCPs. In rotavirus, the transcriptionally competent form of the virus has a double-layered capsid consisting of the structural proteins VP2 and VP6 surrounding the dsRNA genome segments and the enzymatic machinery in the core. Although observation of the actively transcribing rotavirus particle by electron cryomicroscopy has not provided a clear definition of the pathway of mRNA translocation through the inner capsid layer, atomic resolution structural studies of the bluetongue virus TCP have suggested a possible route (Grimes et al., 1998) .	./cache/cord-008575-bbpmlo3c.txt	./txt/cord-008575-bbpmlo3c.txt