id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-007474-ckqghr3b	Johnson, Michael E.	Development of specific nucleic acid probes for the differentiation of porcine rotavirus serotypes	2002-11-13		.txt	text/plain	6487	362	51	Recombinant complementary DNA (cDNA) representing gene 9 (the gene encoding the neutralization antigens in VP7 glycoprotein) from OSU (porcine rotavirus serotype 1) and Gottfried (porcine rotavirus serotype 2) strains were used to determine the optimal hybridization conditions which allow specific detection of group A porcine rotaviruses. In the present study, a dot blot hybridization assay is described for the detection and serotypic differentiation of porcine rotavirus utilizing hybridization probes prepared from recombinant cDNA representing gene 9 from OSU and Gottfried strains (porcine rotavirus serotypes 1 and 2, respectively). Five subfragments of gene 9 from OSU and Gottfried (Table 1 ) were 32p-labeled and hybridized to heterologous rotavirus RNA under both high and low stringency conditions. They reported that rotavirus serotypes 2 and 3 could be differentiated with the gene 9 cDNA probe by using conditions of low stringency (56 ° C), and comparison with the results obtained with hybridization at 56°C, 13% formamide.	./cache/cord-007474-ckqghr3b.txt	./txt/cord-007474-ckqghr3b.txt