id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-003305-ya0siivm	Liu, Weichi	A unique intra-molecular fidelity-modulating mechanism identified in a viral RNA-dependent RNA polymerase	2018-11-16		.txt	text/plain	8861	427	54	The intra-molecular NTD interactions with the RdRP palm that controls the active site closure, together with the observation of the NTD deletion mutant N-91 exhibited higher level of misincorporation in the de novo RNA synthesis, suggesting a unique mode of fidelity modulation. In order to quantitatively assess whether the fidelity levels are modulated by the intra-molecular NTD-RdRP interface interactions, we established the 32 P-radioactivity based NTP misincorporation assays using the T30/P2 construct utilized in the aforementioned de novo-mode synthesis assessment ( Figure 1B and C) . The effect of E472A mutation was highly consistent with AA mutation ( Figure 4B and Supplementary Figure S3A Since polymerase misincorporation level can be affected by the type of misincorporation and the sequence context of the misincorporation site, we established the second type of regular NTP misincorporation assays using the same T30/P2 construct for a more adequate assessment of the RdRP fidelity modulation brought by the NTD.	./cache/cord-003305-ya0siivm.txt	./txt/cord-003305-ya0siivm.txt