id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-000866-dr2uow4m	Picard-Jean, Frédéric	The Immunosuppressive Agent Mizoribine Monophosphate Is an Inhibitor of the Human RNA Capping Enzyme	2013-01-17		.txt	text/plain	7046	368	50	In the present study, we investigated the ability of MZP to directly inhibit the human RNA capping enzyme (HCE), a protein harboring both RNA 5′-triphosphatase and RNA guanylyltransferase activities. An RNA guanylyltransferase (GTase) then catalyzes a two-step reaction in which it initially utilizes GTP as a substrate to form a covalent enzyme-GMP (EpG) intermediate, with the concomitant release of pyrophosphate (PPi). Since ribavirin triphosphate and MZP share functional similarities, we investigated the attractive possibility that MZP could also inhibit the GTase activity of the human RNA capping enzyme (HCE). The enzyme-GMP complex formation assays were carried out for 3 min at 37uC in a buffer containing 1 mM [a-32 P]GTP, 4 mM HCE, 50 mM Tris-HCl, pH 7.5, 5 mM MgCl 2 , 500 mM DTT, 0.5 ng/ml pyrophosphatase (Roche), and various concentration of MZP (as indicated).	./cache/cord-000866-dr2uow4m.txt	./txt/cord-000866-dr2uow4m.txt