id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-000578-jhetyd9t	Kovalev, Nikolay	A Co-Opted DEAD-Box RNA Helicase Enhances Tombusvirus Plus-Strand Synthesis	2012-02-16		.txt	text/plain	8793	509	59	In this paper, we show that an essential translation factor, Ded1p DEAD-box RNA helicase of yeast, directly affects replication of Tomato bushy stunt virus (TBSV). In this paper, the authors show that the Ded1p DEAD-box helicase, which is an essential translation factor in yeast, is recruited by Tomato bushy stunt virus (TBSV) into its replicase complex. Interestingly, addition of purified Ded1p to the tombusvirus replicase assay containing the short RNA/DNA duplex ( Figure 7A Non-overlapping functions of Ded1p and GAPDH in promoting initiation by the tombusvirus replicase GAPDH (Tdh2p in yeast) RNA binding protein is also a host factor stimulating (+)RNA synthesis by the tombusvirus replicase [25, 50] . To test if Ded1p and GAPDH could play a complementary role during (+)RNA synthesis, we added the purified recombinant Ded1p and Tdh2p to the in vitro tombusvirus replicase assay based on the purified preparation ( Figure 8A ).	./cache/cord-000578-jhetyd9t.txt	./txt/cord-000578-jhetyd9t.txt