id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-311035-s3tkbh9r	Procko, Erik	Deep mutagenesis in the study of COVID-19: a technical overview for the proteomics community	2020-10-21		.txt	text/plain	4033	210	45	A deep mutational scan of ACE2 expressed on human cells identified mutations that increase S affinity and guided the engineering of a potent and broad soluble receptor decoy. • The experimental mutational landscape of ACE2 for binding the RBD of SARS-CoV-2 provides a blueprint for engineering high affinity decoy receptors. Following FACS selection of the human culture to enrich a cell population with high binding activity for SARS-CoV-2 protein S, RNA transcripts were isolated and Illumina sequenced. The deep mutational scan of ACE2 revealed that mutations can indeed be found to enhance binding toward SARS-CoV-2 RBD (Figure 2) , suitable for engineering high affinity soluble decoy receptors [15] . A soluble ACE2 variant that combines three mutations, called sACE2 2 .v2.4, was found to be highly expressed, is a stable monodisperse dimer, binds SARS-CoV-2 S with picomolar affinity and potently neutralizes infection of a susceptible cell line by authentic virus.	./cache/cord-311035-s3tkbh9r.txt	./txt/cord-311035-s3tkbh9r.txt