id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-024319-isbqs7hg	Zeng, Xin	Isolation of a human monoclonal antibody specific for the receptor binding domain of SARS-CoV-2 using a competitive phage biopanning strategy	2020-04-30		.txt	text/plain	3036	174	55	title: Isolation of a human monoclonal antibody specific for the receptor binding domain of SARS-CoV-2 using a competitive phage biopanning strategy SARS-CoV-2 relies on its spike protein, in particular the receptor binding domain (RBD), to bind human cell receptor angiotensin-converting enzyme 2 (ACE2) for viral entry, and thus targeting RBD holds the promise for preventing SARS-CoV-2 infection. It was proved to competitively block the binding of RBD to ACE2 and potently inhibit SARS-CoV-2 pseudovirus infection with IC(50) values of 12 nM. Several high-affinity antibodies targeting SARS-CoV-2 RBD and blocking its binding to ACE2 were isolated, and the top 1 lead exhibited a neutralization activity of SARS-CoV-2 pseudotyped VSV infection. These antibodies all compete with ACE2 to bind SARS-CoV RBD, but their epitopes only have limited overlaps of the Two SARS-CoV-2 RBD-specific antibodies selected from different strategies showed different neutralization activities.	./cache/cord-024319-isbqs7hg.txt	./txt/cord-024319-isbqs7hg.txt