id sid tid token lemma pos cord-022235-6ircruag 1 1 key key JJ cord-022235-6ircruag 1 2 : : : cord-022235-6ircruag 1 3 cord-022235 cord-022235 NNP cord-022235-6ircruag 1 4 - - HYPH cord-022235-6ircruag 1 5 6ircruag 6ircruag CD cord-022235-6ircruag 2 1 authors author NNS cord-022235-6ircruag 2 2 : : : cord-022235-6ircruag 2 3 Pugsley Pugsley NNP cord-022235-6ircruag 2 4 , , , cord-022235-6ircruag 2 5 Anthony Anthony NNP cord-022235-6ircruag 2 6 P. P. NNP cord-022235-6ircruag 2 7 title title NN cord-022235-6ircruag 2 8 : : : cord-022235-6ircruag 3 1 Later later RB cord-022235-6ircruag 3 2 stages stage NNS cord-022235-6ircruag 3 3 in in IN cord-022235-6ircruag 3 4 the the DT cord-022235-6ircruag 3 5 eukaryotic eukaryotic JJ cord-022235-6ircruag 3 6 secretory secretory JJ cord-022235-6ircruag 3 7 pathway pathway NN cord-022235-6ircruag 3 8 date date NN cord-022235-6ircruag 3 9 : : : cord-022235-6ircruag 3 10 2012 2012 CD cord-022235-6ircruag 3 11 - - SYM cord-022235-6ircruag 3 12 12 12 CD cord-022235-6ircruag 3 13 - - SYM cord-022235-6ircruag 3 14 02 02 CD cord-022235-6ircruag 3 15 journal journal NN cord-022235-6ircruag 3 16 : : : cord-022235-6ircruag 4 1 Protein protein NN cord-022235-6ircruag 4 2 Targeting Targeting NNP cord-022235-6ircruag 4 3 DOI DOI NNP cord-022235-6ircruag 4 4 : : : cord-022235-6ircruag 4 5 10.1016 10.1016 CD cord-022235-6ircruag 4 6 / / SYM cord-022235-6ircruag 4 7 b978 b978 NN cord-022235-6ircruag 4 8 - - HYPH cord-022235-6ircruag 4 9 0 0 CD cord-022235-6ircruag 4 10 - - HYPH cord-022235-6ircruag 4 11 12 12 CD cord-022235-6ircruag 4 12 - - HYPH cord-022235-6ircruag 4 13 566770 566770 CD cord-022235-6ircruag 4 14 - - HYPH cord-022235-6ircruag 4 15 8.50009 8.50009 CD cord-022235-6ircruag 4 16 - - SYM cord-022235-6ircruag 4 17 4 4 CD cord-022235-6ircruag 4 18 sha sha NNP cord-022235-6ircruag 4 19 : : : cord-022235-6ircruag 5 1 b2acf257e97d8fb539f0a68c348c2559271ba932 b2acf257e97d8fb539f0a68c348c2559271ba932 RB cord-022235-6ircruag 6 1 doc_id doc_id CD cord-022235-6ircruag 6 2 : : : cord-022235-6ircruag 6 3 22235 22235 CD cord-022235-6ircruag 6 4 cord_uid cord_uid NNS cord-022235-6ircruag 6 5 : : : cord-022235-6ircruag 7 1 6ircruag 6ircruag NNP cord-022235-6ircruag 7 2 nan nan NNP cord-022235-6ircruag 8 1 The the DT cord-022235-6ircruag 8 2 secretory secretory JJ cord-022235-6ircruag 8 3 pathway pathway NN cord-022235-6ircruag 8 4 of of IN cord-022235-6ircruag 8 5 eukaryotic eukaryotic JJ cord-022235-6ircruag 8 6 cells cell NNS cord-022235-6ircruag 8 7 comprises comprise VBZ cord-022235-6ircruag 8 8 a a DT cord-022235-6ircruag 8 9 succession succession NN cord-022235-6ircruag 8 10 of of IN cord-022235-6ircruag 8 11 compartments compartment NNS cord-022235-6ircruag 8 12 , , , cord-022235-6ircruag 8 13 the the DT cord-022235-6ircruag 8 14 secretory secretory JJ cord-022235-6ircruag 8 15 organelles organelle NNS cord-022235-6ircruag 8 16 , , , cord-022235-6ircruag 8 17 through through IN cord-022235-6ircruag 8 18 which which WDT cord-022235-6ircruag 8 19 proteins protein NNS cord-022235-6ircruag 8 20 pass pass VBP cord-022235-6ircruag 8 21 en en IN cord-022235-6ircruag 8 22 route route NN cord-022235-6ircruag 8 23 to to IN cord-022235-6ircruag 8 24 their -PRON- PRP$ cord-022235-6ircruag 8 25 final final JJ cord-022235-6ircruag 8 26 destinations destination NNS cord-022235-6ircruag 8 27 . . . cord-022235-6ircruag 9 1 Although although IN cord-022235-6ircruag 9 2 each each DT cord-022235-6ircruag 9 3 secretory secretory JJ cord-022235-6ircruag 9 4 organelle organelle NN cord-022235-6ircruag 9 5 has have VBZ cord-022235-6ircruag 9 6 its -PRON- PRP$ cord-022235-6ircruag 9 7 own own JJ cord-022235-6ircruag 9 8 special special JJ cord-022235-6ircruag 9 9 characteristics characteristic NNS cord-022235-6ircruag 9 10 , , , cord-022235-6ircruag 9 11 a a DT cord-022235-6ircruag 9 12 number number NN cord-022235-6ircruag 9 13 of of IN cord-022235-6ircruag 9 14 basic basic JJ cord-022235-6ircruag 9 15 features feature NNS cord-022235-6ircruag 9 16 are be VBP cord-022235-6ircruag 9 17 common common JJ cord-022235-6ircruag 9 18 to to IN cord-022235-6ircruag 9 19 all all DT cord-022235-6ircruag 9 20 of of IN cord-022235-6ircruag 9 21 them -PRON- PRP cord-022235-6ircruag 9 22 . . . cord-022235-6ircruag 10 1 Some some DT cord-022235-6ircruag 10 2 proteins protein NNS cord-022235-6ircruag 10 3 pass pass VBP cord-022235-6ircruag 10 4 more more RBR cord-022235-6ircruag 10 5 or or CC cord-022235-6ircruag 10 6 less less RBR cord-022235-6ircruag 10 7 unimpeded unimpeded JJ cord-022235-6ircruag 10 8 through through IN cord-022235-6ircruag 10 9 the the DT cord-022235-6ircruag 10 10 entire entire JJ cord-022235-6ircruag 10 11 length length NN cord-022235-6ircruag 10 12 of of IN cord-022235-6ircruag 10 13 the the DT cord-022235-6ircruag 10 14 secretory secretory JJ cord-022235-6ircruag 10 15 pathway pathway NN cord-022235-6ircruag 10 16 , , , cord-022235-6ircruag 10 17 whereas whereas IN cord-022235-6ircruag 10 18 others other NNS cord-022235-6ircruag 10 19 are be VBP cord-022235-6ircruag 10 20 retained retain VBN cord-022235-6ircruag 10 21 in in IN cord-022235-6ircruag 10 22 secretory secretory JJ cord-022235-6ircruag 10 23 organelles organelle NNS cord-022235-6ircruag 10 24 . . . cord-022235-6ircruag 11 1 This this DT cord-022235-6ircruag 11 2 raises raise VBZ cord-022235-6ircruag 11 3 two two CD cord-022235-6ircruag 11 4 of of IN cord-022235-6ircruag 11 5 the the DT cord-022235-6ircruag 11 6 fundamental fundamental JJ cord-022235-6ircruag 11 7 questions question NNS cord-022235-6ircruag 11 8 which which WDT cord-022235-6ircruag 11 9 will will MD cord-022235-6ircruag 11 10 be be VB cord-022235-6ircruag 11 11 addressed address VBN cord-022235-6ircruag 11 12 in in IN cord-022235-6ircruag 11 13 this this DT cord-022235-6ircruag 11 14 chapter chapter NN cord-022235-6ircruag 11 15 , , , cord-022235-6ircruag 11 16 namely namely RB cord-022235-6ircruag 11 17 , , , cord-022235-6ircruag 11 18 what what WP cord-022235-6ircruag 11 19 determines determine VBZ cord-022235-6ircruag 11 20 whether whether IN cord-022235-6ircruag 11 21 a a DT cord-022235-6ircruag 11 22 protein protein NN cord-022235-6ircruag 11 23 will will MD cord-022235-6ircruag 11 24 be be VB cord-022235-6ircruag 11 25 taken take VBN cord-022235-6ircruag 11 26 out out IN cord-022235-6ircruag 11 27 of of IN cord-022235-6ircruag 11 28 circulation circulation NN cord-022235-6ircruag 11 29 at at IN cord-022235-6ircruag 11 30 a a DT cord-022235-6ircruag 11 31 particular particular JJ cord-022235-6ircruag 11 32 step step NN cord-022235-6ircruag 11 33 in in IN cord-022235-6ircruag 11 34 the the DT cord-022235-6ircruag 11 35 pathway pathway NN cord-022235-6ircruag 11 36 , , , cord-022235-6ircruag 11 37 and and CC cord-022235-6ircruag 11 38 how how WRB cord-022235-6ircruag 11 39 tight tight JJ cord-022235-6ircruag 11 40 is be VBZ cord-022235-6ircruag 11 41 the the DT cord-022235-6ircruag 11 42 separation separation NN cord-022235-6ircruag 11 43 between between IN cord-022235-6ircruag 11 44 secretory secretory JJ cord-022235-6ircruag 11 45 organelles organelle NNS cord-022235-6ircruag 11 46 ? ? . cord-022235-6ircruag 12 1 Ultrastructure ultrastructure JJ cord-022235-6ircruag 12 2 and and CC cord-022235-6ircruag 12 3 cell cell NN cord-022235-6ircruag 12 4 fractionation fractionation NN cord-022235-6ircruag 12 5 studies study NNS cord-022235-6ircruag 12 6 indicate indicate VBP cord-022235-6ircruag 12 7 that that IN cord-022235-6ircruag 12 8 there there EX cord-022235-6ircruag 12 9 is be VBZ cord-022235-6ircruag 12 10 little little JJ cord-022235-6ircruag 12 11 or or CC cord-022235-6ircruag 12 12 no no DT cord-022235-6ircruag 12 13 physical physical JJ cord-022235-6ircruag 12 14 link link NN cord-022235-6ircruag 12 15 between between IN cord-022235-6ircruag 12 16 the the DT cord-022235-6ircruag 12 17 RER rer NN cord-022235-6ircruag 12 18 and and CC cord-022235-6ircruag 12 19 the the DT cord-022235-6ircruag 12 20 Golgi Golgi NNP cord-022235-6ircruag 12 21 . . . cord-022235-6ircruag 13 1 Furthermore furthermore RB cord-022235-6ircruag 13 2 , , , cord-022235-6ircruag 13 3 specific specific JJ cord-022235-6ircruag 13 4 proteins protein NNS cord-022235-6ircruag 13 5 are be VBP cord-022235-6ircruag 13 6 known know VBN cord-022235-6ircruag 13 7 to to TO cord-022235-6ircruag 13 8 reside reside VB cord-022235-6ircruag 13 9 in in IN cord-022235-6ircruag 13 10 individual individual JJ cord-022235-6ircruag 13 11 compartments compartment NNS cord-022235-6ircruag 13 12 of of IN cord-022235-6ircruag 13 13 the the DT cord-022235-6ircruag 13 14 Golgi Golgi NNP cord-022235-6ircruag 13 15 apparatus apparatus NN cord-022235-6ircruag 13 16 . . . cord-022235-6ircruag 14 1 As as IN cord-022235-6ircruag 14 2 we -PRON- PRP cord-022235-6ircruag 14 3 shall shall MD cord-022235-6ircruag 14 4 see see VB cord-022235-6ircruag 14 5 , , , cord-022235-6ircruag 14 6 the the DT cord-022235-6ircruag 14 7 physical physical JJ cord-022235-6ircruag 14 8 separation separation NN cord-022235-6ircruag 14 9 of of IN cord-022235-6ircruag 14 10 Golgi Golgi NNP cord-022235-6ircruag 14 11 enzymes enzyme NNS cord-022235-6ircruag 14 12 is be VBZ cord-022235-6ircruag 14 13 also also RB cord-022235-6ircruag 14 14 indicated indicate VBN cord-022235-6ircruag 14 15 by by IN cord-022235-6ircruag 14 16 the the DT cord-022235-6ircruag 14 17 succession succession NN cord-022235-6ircruag 14 18 of of IN cord-022235-6ircruag 14 19 posttranslational posttranslational JJ cord-022235-6ircruag 14 20 modifications modification NNS cord-022235-6ircruag 14 21 to to IN cord-022235-6ircruag 14 22 which which WDT cord-022235-6ircruag 14 23 secretory secretory JJ cord-022235-6ircruag 14 24 proteins protein NNS cord-022235-6ircruag 14 25 are be VBP cord-022235-6ircruag 14 26 subjected subject VBN cord-022235-6ircruag 14 27 , , , cord-022235-6ircruag 14 28 by by IN cord-022235-6ircruag 14 29 in in IN cord-022235-6ircruag 14 30 situ situ NN cord-022235-6ircruag 14 31 immunocytochemistry immunocytochemistry NN cord-022235-6ircruag 14 32 , , , cord-022235-6ircruag 14 33 and and CC cord-022235-6ircruag 14 34 by by IN cord-022235-6ircruag 14 35 the the DT cord-022235-6ircruag 14 36 separation separation NN cord-022235-6ircruag 14 37 of of IN cord-022235-6ircruag 14 38 Golgi golgi NN cord-022235-6ircruag 14 39 - - HYPH cord-022235-6ircruag 14 40 derived derive VBN cord-022235-6ircruag 14 41 vesicles vesicle NNS cord-022235-6ircruag 14 42 containing contain VBG cord-022235-6ircruag 14 43 different different JJ cord-022235-6ircruag 14 44 en en IN cord-022235-6ircruag 14 45 zymes zymes NNP cord-022235-6ircruag 14 46 . . . cord-022235-6ircruag 15 1 How how WRB cord-022235-6ircruag 15 2 then then RB cord-022235-6ircruag 15 3 do do VBP cord-022235-6ircruag 15 4 secretory secretory JJ cord-022235-6ircruag 15 5 proteins protein NNS cord-022235-6ircruag 15 6 move move VBP cord-022235-6ircruag 15 7 between between IN cord-022235-6ircruag 15 8 these these DT cord-022235-6ircruag 15 9 compart compart NN cord-022235-6ircruag 15 10 ments ment NNS cord-022235-6ircruag 15 11 , , , cord-022235-6ircruag 15 12 and and CC cord-022235-6ircruag 15 13 is be VBZ cord-022235-6ircruag 15 14 the the DT cord-022235-6ircruag 15 15 secretory secretory JJ cord-022235-6ircruag 15 16 pathway pathway NN cord-022235-6ircruag 15 17 a a DT cord-022235-6ircruag 15 18 continuous continuous JJ cord-022235-6ircruag 15 19 gradient gradient NN cord-022235-6ircruag 15 20 of of IN cord-022235-6ircruag 15 21 secretory secretory JJ cord-022235-6ircruag 15 22 organelles organelle NNS cord-022235-6ircruag 15 23 or or CC cord-022235-6ircruag 15 24 are be VBP cord-022235-6ircruag 15 25 they -PRON- PRP cord-022235-6ircruag 15 26 functionally functionally RB cord-022235-6ircruag 15 27 and and CC cord-022235-6ircruag 15 28 structurally structurally RB cord-022235-6ircruag 15 29 independent independent JJ cord-022235-6ircruag 15 30 ? ? . cord-022235-6ircruag 16 1 A a DT cord-022235-6ircruag 16 2 feature feature NN cord-022235-6ircruag 16 3 common common JJ cord-022235-6ircruag 16 4 to to IN cord-022235-6ircruag 16 5 all all DT cord-022235-6ircruag 16 6 stages stage NNS cord-022235-6ircruag 16 7 of of IN cord-022235-6ircruag 16 8 the the DT cord-022235-6ircruag 16 9 secretory secretory JJ cord-022235-6ircruag 16 10 route route NN cord-022235-6ircruag 16 11 beyond beyond IN cord-022235-6ircruag 16 12 the the DT cord-022235-6ircruag 16 13 RER rer NN cord-022235-6ircruag 16 14 is be VBZ cord-022235-6ircruag 16 15 that that IN cord-022235-6ircruag 16 16 proteins protein NNS cord-022235-6ircruag 16 17 move move VBP cord-022235-6ircruag 16 18 between between IN cord-022235-6ircruag 16 19 secretory secretory JJ cord-022235-6ircruag 16 20 organelles organelle NNS cord-022235-6ircruag 16 21 in in IN cord-022235-6ircruag 16 22 specific specific JJ cord-022235-6ircruag 16 23 classes class NNS cord-022235-6ircruag 16 24 of of IN cord-022235-6ircruag 16 25 transport transport NN cord-022235-6ircruag 16 26 vesicles vesicle NNS cord-022235-6ircruag 16 27 . . . cord-022235-6ircruag 17 1 Consequently consequently RB cord-022235-6ircruag 17 2 , , , cord-022235-6ircruag 17 3 soluble soluble JJ cord-022235-6ircruag 17 4 secretory secretory NN cord-022235-6ircruag 17 5 proteins protein NNS cord-022235-6ircruag 17 6 never never RB cord-022235-6ircruag 17 7 come come VBP cord-022235-6ircruag 17 8 into into IN cord-022235-6ircruag 17 9 direct direct JJ cord-022235-6ircruag 17 10 contact contact NN cord-022235-6ircruag 17 11 with with IN cord-022235-6ircruag 17 12 the the DT cord-022235-6ircruag 17 13 outer outer JJ cord-022235-6ircruag 17 14 face face NN cord-022235-6ircruag 17 15 of of IN cord-022235-6ircruag 17 16 the the DT cord-022235-6ircruag 17 17 organelle organelle NN cord-022235-6ircruag 17 18 to to TO cord-022235-6ircruag 17 19 which which WDT cord-022235-6ircruag 17 20 they -PRON- PRP cord-022235-6ircruag 17 21 are be VBP cord-022235-6ircruag 17 22 being be VBG cord-022235-6ircruag 17 23 targeted target VBN cord-022235-6ircruag 17 24 and and CC cord-022235-6ircruag 17 25 therefore therefore RB cord-022235-6ircruag 17 26 can can MD cord-022235-6ircruag 17 27 play play VB cord-022235-6ircruag 17 28 no no DT cord-022235-6ircruag 17 29 direct direct JJ cord-022235-6ircruag 17 30 part part NN cord-022235-6ircruag 17 31 in in IN cord-022235-6ircruag 17 32 sorting sorting NN cord-022235-6ircruag 17 33 . . . cord-022235-6ircruag 18 1 Integral integral JJ cord-022235-6ircruag 18 2 membrane membrane NN cord-022235-6ircruag 18 3 proteins protein NNS cord-022235-6ircruag 18 4 usually usually RB cord-022235-6ircruag 18 5 have have VBP cord-022235-6ircruag 18 6 segments segment NNS cord-022235-6ircruag 18 7 exposed expose VBN cord-022235-6ircruag 18 8 on on IN cord-022235-6ircruag 18 9 the the DT cord-022235-6ircruag 18 10 cytoplasmic cytoplasmic JJ cord-022235-6ircruag 18 11 face face NN cord-022235-6ircruag 18 12 of of IN cord-022235-6ircruag 18 13 the the DT cord-022235-6ircruag 18 14 transport transport NN cord-022235-6ircruag 18 15 vesicle vesicle NN cord-022235-6ircruag 18 16 which which WDT cord-022235-6ircruag 18 17 could could MD cord-022235-6ircruag 18 18 be be VB cord-022235-6ircruag 18 19 recognized recognize VBN cord-022235-6ircruag 18 20 by by IN cord-022235-6ircruag 18 21 receptors receptor NNS cord-022235-6ircruag 18 22 on on IN cord-022235-6ircruag 18 23 the the DT cord-022235-6ircruag 18 24 target target NN cord-022235-6ircruag 18 25 organelle organelle NN cord-022235-6ircruag 18 26 . . . cord-022235-6ircruag 19 1 Microinjected Microinjected NNP cord-022235-6ircruag 19 2 antibodies antibody NNS cord-022235-6ircruag 19 3 recognizing recognize VBG cord-022235-6ircruag 19 4 the the DT cord-022235-6ircruag 19 5 C C NNP cord-022235-6ircruag 19 6 - - HYPH cord-022235-6ircruag 19 7 terminal terminal NN cord-022235-6ircruag 19 8 , , , cord-022235-6ircruag 19 9 cytoplasmic cytoplasmic JJ cord-022235-6ircruag 19 10 tails tail NNS cord-022235-6ircruag 19 11 of of IN cord-022235-6ircruag 19 12 plasma plasma NN cord-022235-6ircruag 19 13 membrane membrane NN cord-022235-6ircruag 19 14 proteins protein NNS cord-022235-6ircruag 19 15 can can MD cord-022235-6ircruag 19 16 prevent prevent VB cord-022235-6ircruag 19 17 their -PRON- PRP$ cord-022235-6ircruag 19 18 trans trans JJ cord-022235-6ircruag 19 19 port port NN cord-022235-6ircruag 19 20 to to IN cord-022235-6ircruag 19 21 the the DT cord-022235-6ircruag 19 22 cell cell NN cord-022235-6ircruag 19 23 surface surface NN cord-022235-6ircruag 19 24 ( ( -LRB- cord-022235-6ircruag 19 25 25 25 CD cord-022235-6ircruag 19 26 , , , cord-022235-6ircruag 19 27 590 590 CD cord-022235-6ircruag 19 28 ) ) -RRB- cord-022235-6ircruag 19 29 , , , cord-022235-6ircruag 19 30 but but CC cord-022235-6ircruag 19 31 this this DT cord-022235-6ircruag 19 32 is be VBZ cord-022235-6ircruag 19 33 probably probably RB cord-022235-6ircruag 19 34 due due JJ cord-022235-6ircruag 19 35 to to IN cord-022235-6ircruag 19 36 antibodyinduced antibodyinduced JJ cord-022235-6ircruag 19 37 changes change NNS cord-022235-6ircruag 19 38 in in IN cord-022235-6ircruag 19 39 protein protein NN cord-022235-6ircruag 19 40 conformation conformation NN cord-022235-6ircruag 19 41 which which WDT cord-022235-6ircruag 19 42 make make VBP cord-022235-6ircruag 19 43 the the DT cord-022235-6ircruag 19 44 protein protein NN cord-022235-6ircruag 19 45 incom incom NNP cord-022235-6ircruag 19 46 petent petent NN cord-022235-6ircruag 19 47 for for IN cord-022235-6ircruag 19 48 transport transport NN cord-022235-6ircruag 19 49 along along IN cord-022235-6ircruag 19 50 the the DT cord-022235-6ircruag 19 51 secretory secretory JJ cord-022235-6ircruag 19 52 pathway pathway NN cord-022235-6ircruag 19 53 , , , cord-022235-6ircruag 19 54 rather rather RB cord-022235-6ircruag 19 55 than than IN cord-022235-6ircruag 19 56 to to IN cord-022235-6ircruag 19 57 inhibition inhibition NN cord-022235-6ircruag 19 58 of of IN cord-022235-6ircruag 19 59 receptor receptor NN cord-022235-6ircruag 19 60 - - HYPH cord-022235-6ircruag 19 61 secretory secretory NN cord-022235-6ircruag 19 62 protein protein NN cord-022235-6ircruag 19 63 interactions interaction NNS cord-022235-6ircruag 19 64 . . . cord-022235-6ircruag 20 1 In in IN cord-022235-6ircruag 20 2 this this DT cord-022235-6ircruag 20 3 chapter chapter NN cord-022235-6ircruag 20 4 , , , cord-022235-6ircruag 20 5 we -PRON- PRP cord-022235-6ircruag 20 6 will will MD cord-022235-6ircruag 20 7 consider consider VB cord-022235-6ircruag 20 8 three three CD cord-022235-6ircruag 20 9 different different JJ cord-022235-6ircruag 20 10 ways way NNS cord-022235-6ircruag 20 11 in in IN cord-022235-6ircruag 20 12 which which WDT cord-022235-6ircruag 20 13 sorting sorting NN cord-022235-6ircruag 20 14 of of IN cord-022235-6ircruag 20 15 secretory secretory JJ cord-022235-6ircruag 20 16 proteins protein NNS cord-022235-6ircruag 20 17 might may MD cord-022235-6ircruag 20 18 occur occur VB cord-022235-6ircruag 20 19 : : : cord-022235-6ircruag 20 20 ( ( -LRB- cord-022235-6ircruag 20 21 i i NN cord-022235-6ircruag 20 22 ) ) -RRB- cord-022235-6ircruag 21 1 All all DT cord-022235-6ircruag 21 2 secretory secretory JJ cord-022235-6ircruag 21 3 proteins protein NNS cord-022235-6ircruag 21 4 have have VBP cord-022235-6ircruag 21 5 signals signal NNS cord-022235-6ircruag 21 6 which which WDT cord-022235-6ircruag 21 7 target target VBP cord-022235-6ircruag 21 8 them -PRON- PRP cord-022235-6ircruag 21 9 successively successively RB cord-022235-6ircruag 21 10 through through IN cord-022235-6ircruag 21 11 the the DT cord-022235-6ircruag 21 12 secretory secretory JJ cord-022235-6ircruag 21 13 organelles organelle NNS cord-022235-6ircruag 21 14 and and CC cord-022235-6ircruag 21 15 then then RB cord-022235-6ircruag 21 16 on on RB cord-022235-6ircruag 21 17 to to IN cord-022235-6ircruag 21 18 their -PRON- PRP$ cord-022235-6ircruag 21 19 final final JJ cord-022235-6ircruag 21 20 target target NN cord-022235-6ircruag 21 21 ; ; : cord-022235-6ircruag 21 22 some some DT cord-022235-6ircruag 21 23 proteins protein NNS cord-022235-6ircruag 21 24 remain remain VBP cord-022235-6ircruag 21 25 in in IN cord-022235-6ircruag 21 26 different different JJ cord-022235-6ircruag 21 27 organelles organelle NNS cord-022235-6ircruag 21 28 because because IN cord-022235-6ircruag 21 29 they -PRON- PRP cord-022235-6ircruag 21 30 lack lack VBP cord-022235-6ircruag 21 31 the the DT cord-022235-6ircruag 21 32 signal signal NN cord-022235-6ircruag 21 33 necessary necessary JJ cord-022235-6ircruag 21 34 for for IN cord-022235-6ircruag 21 35 targeting target VBG cord-022235-6ircruag 21 36 to to IN cord-022235-6ircruag 21 37 the the DT cord-022235-6ircruag 21 38 next next JJ cord-022235-6ircruag 21 39 organelle organelle NN cord-022235-6ircruag 21 40 in in IN cord-022235-6ircruag 21 41 the the DT cord-022235-6ircruag 21 42 pathway pathway NN cord-022235-6ircruag 21 43 . . . cord-022235-6ircruag 22 1 ( ( -LRB- cord-022235-6ircruag 22 2 ii ii LS cord-022235-6ircruag 22 3 ) ) -RRB- cord-022235-6ircruag 22 4 Secretory secretory NN cord-022235-6ircruag 22 5 proteins protein NNS cord-022235-6ircruag 22 6 only only RB cord-022235-6ircruag 22 7 have have VBP cord-022235-6ircruag 22 8 sorting sorting NN cord-022235-6ircruag 22 9 signals signal NNS cord-022235-6ircruag 22 10 for for IN cord-022235-6ircruag 22 11 the the DT cord-022235-6ircruag 22 12 last last JJ cord-022235-6ircruag 22 13 stage stage NN cord-022235-6ircruag 22 14 in in IN cord-022235-6ircruag 22 15 the the DT cord-022235-6ircruag 22 16 secretory secretory JJ cord-022235-6ircruag 22 17 pathway pathway NN cord-022235-6ircruag 22 18 ; ; : cord-022235-6ircruag 22 19 some some DT cord-022235-6ircruag 22 20 proteins protein NNS cord-022235-6ircruag 22 21 have have VBP cord-022235-6ircruag 22 22 retention retention NN cord-022235-6ircruag 22 23 signals signal NNS cord-022235-6ircruag 22 24 which which WDT cord-022235-6ircruag 22 25 pre pre VBD cord-022235-6ircruag 22 26 vent vent VB cord-022235-6ircruag 22 27 them -PRON- PRP cord-022235-6ircruag 22 28 joining join VBG cord-022235-6ircruag 22 29 the the DT cord-022235-6ircruag 22 30 bulk bulk JJ cord-022235-6ircruag 22 31 flow flow NN cord-022235-6ircruag 22 32 through through IN cord-022235-6ircruag 22 33 the the DT cord-022235-6ircruag 22 34 secretory secretory JJ cord-022235-6ircruag 22 35 pathway pathway NN cord-022235-6ircruag 22 36 , , , cord-022235-6ircruag 22 37 thereby thereby RB cord-022235-6ircruag 22 38 causing cause VBG cord-022235-6ircruag 22 39 them -PRON- PRP cord-022235-6ircruag 22 40 to to TO cord-022235-6ircruag 22 41 be be VB cord-022235-6ircruag 22 42 retained retain VBN cord-022235-6ircruag 22 43 in in IN cord-022235-6ircruag 22 44 specific specific JJ cord-022235-6ircruag 22 45 organelles organelle NNS cord-022235-6ircruag 22 46 . . . cord-022235-6ircruag 23 1 ( ( -LRB- cord-022235-6ircruag 23 2 iii iii NN cord-022235-6ircruag 23 3 ) ) -RRB- cord-022235-6ircruag 24 1 As as IN cord-022235-6ircruag 24 2 ( ( -LRB- cord-022235-6ircruag 24 3 ii ii NN cord-022235-6ircruag 24 4 ) ) -RRB- cord-022235-6ircruag 24 5 , , , cord-022235-6ircruag 24 6 except except IN cord-022235-6ircruag 24 7 that that DT cord-022235-6ircruag 24 8 secretory secretory JJ cord-022235-6ircruag 24 9 organelle organelle NN cord-022235-6ircruag 24 10 proteins protein NNS cord-022235-6ircruag 24 11 pass pass VBP cord-022235-6ircruag 24 12 through through IN cord-022235-6ircruag 24 13 the the DT cord-022235-6ircruag 24 14 secretory secretory JJ cord-022235-6ircruag 24 15 pathway pathway NN cord-022235-6ircruag 24 16 in in IN cord-022235-6ircruag 24 17 the the DT cord-022235-6ircruag 24 18 bulk bulk JJ cord-022235-6ircruag 24 19 flow flow NN cord-022235-6ircruag 24 20 and and CC cord-022235-6ircruag 24 21 are be VBP cord-022235-6ircruag 24 22 recycled recycle VBN cord-022235-6ircruag 24 23 via via IN cord-022235-6ircruag 24 24 signaldependent signaldependent JJ cord-022235-6ircruag 24 25 counterflow counterflow NN cord-022235-6ircruag 24 26 . . . cord-022235-6ircruag 25 1 If if IN cord-022235-6ircruag 25 2 either either DT cord-022235-6ircruag 25 3 of of IN cord-022235-6ircruag 25 4 the the DT cord-022235-6ircruag 25 5 last last JJ cord-022235-6ircruag 25 6 two two CD cord-022235-6ircruag 25 7 models model NNS cord-022235-6ircruag 25 8 are be VBP cord-022235-6ircruag 25 9 correct correct JJ cord-022235-6ircruag 25 10 , , , cord-022235-6ircruag 25 11 then then RB cord-022235-6ircruag 25 12 molecules molecule NNS cord-022235-6ircruag 25 13 devoid devoid JJ cord-022235-6ircruag 25 14 of of IN cord-022235-6ircruag 25 15 retention retention NN cord-022235-6ircruag 25 16 or or CC cord-022235-6ircruag 25 17 sorting sorting NN cord-022235-6ircruag 25 18 signals signal NNS cord-022235-6ircruag 25 19 should should MD cord-022235-6ircruag 25 20 travel travel VB cord-022235-6ircruag 25 21 through through IN cord-022235-6ircruag 25 22 the the DT cord-022235-6ircruag 25 23 secretory secretory JJ cord-022235-6ircruag 25 24 pathway pathway NN cord-022235-6ircruag 25 25 as as IN cord-022235-6ircruag 25 26 part part NN cord-022235-6ircruag 25 27 of of IN cord-022235-6ircruag 25 28 the the DT cord-022235-6ircruag 25 29 bulk bulk JJ cord-022235-6ircruag 25 30 flow flow NN cord-022235-6ircruag 25 31 . . . cord-022235-6ircruag 26 1 The the DT cord-022235-6ircruag 26 2 rate rate NN cord-022235-6ircruag 26 3 at at IN cord-022235-6ircruag 26 4 which which WDT cord-022235-6ircruag 26 5 molecules molecule NNS cord-022235-6ircruag 26 6 are be VBP cord-022235-6ircruag 26 7 transported transport VBN cord-022235-6ircruag 26 8 out out IN cord-022235-6ircruag 26 9 of of IN cord-022235-6ircruag 26 10 the the DT cord-022235-6ircruag 26 11 cell cell NN cord-022235-6ircruag 26 12 will will MD cord-022235-6ircruag 26 13 thus thus RB cord-022235-6ircruag 26 14 be be VB cord-022235-6ircruag 26 15 determined determine VBN cord-022235-6ircruag 26 16 by by IN cord-022235-6ircruag 26 17 the the DT cord-022235-6ircruag 26 18 rate rate NN cord-022235-6ircruag 26 19 at at IN cord-022235-6ircruag 26 20 which which WDT cord-022235-6ircruag 26 21 they -PRON- PRP cord-022235-6ircruag 26 22 diffuse diffuse VBP cord-022235-6ircruag 26 23 to to IN cord-022235-6ircruag 26 24 sites site NNS cord-022235-6ircruag 26 25 within within IN cord-022235-6ircruag 26 26 the the DT cord-022235-6ircruag 26 27 RER RER NNP cord-022235-6ircruag 26 28 and and CC cord-022235-6ircruag 26 29 Golgi Golgi NNP cord-022235-6ircruag 26 30 where where WRB cord-022235-6ircruag 26 31 transport transport NN cord-022235-6ircruag 26 32 vesicles vesicle NNS cord-022235-6ircruag 26 33 are be VBP cord-022235-6ircruag 26 34 formed form VBN cord-022235-6ircruag 26 35 and and CC cord-022235-6ircruag 26 36 depart depart VB cord-022235-6ircruag 26 37 en en IN cord-022235-6ircruag 26 38 route route NN cord-022235-6ircruag 26 39 to to IN cord-022235-6ircruag 26 40 the the DT cord-022235-6ircruag 26 41 next next JJ cord-022235-6ircruag 26 42 compartment compartment NN cord-022235-6ircruag 26 43 in in IN cord-022235-6ircruag 26 44 the the DT cord-022235-6ircruag 26 45 chain chain NN cord-022235-6ircruag 26 46 . . . cord-022235-6ircruag 27 1 This this DT cord-022235-6ircruag 27 2 flow flow NN cord-022235-6ircruag 27 3 rate rate NN cord-022235-6ircruag 27 4 has have VBZ cord-022235-6ircruag 27 5 recently recently RB cord-022235-6ircruag 27 6 been be VBN cord-022235-6ircruag 27 7 measured measure VBN cord-022235-6ircruag 27 8 with with IN cord-022235-6ircruag 27 9 the the DT cord-022235-6ircruag 27 10 N n CD cord-022235-6ircruag 27 11 - - HYPH cord-022235-6ircruag 27 12 glycosylation glycosylation NN cord-022235-6ircruag 27 13 acceptor acceptor NN cord-022235-6ircruag 27 14 tripeptide tripeptide NN cord-022235-6ircruag 27 15 Asn Asn NNP cord-022235-6ircruag 27 16 - - HYPH cord-022235-6ircruag 27 17 Tyr Tyr NNP cord-022235-6ircruag 27 18 - - HYPH cord-022235-6ircruag 27 19 Thr Thr NNP cord-022235-6ircruag 27 20 ( ( -LRB- cord-022235-6ircruag 27 21 Section section NN cord-022235-6ircruag 27 22 V.B.I V.B.I NNP cord-022235-6ircruag 27 23 ) ) -RRB- cord-022235-6ircruag 27 24 . . . cord-022235-6ircruag 28 1 If if IN cord-022235-6ircruag 28 2 this this DT cord-022235-6ircruag 28 3 tripeptide tripeptide NN cord-022235-6ircruag 28 4 contains contain VBZ cord-022235-6ircruag 28 5 a a DT cord-022235-6ircruag 28 6 radioiodinated radioiodinated JJ cord-022235-6ircruag 28 7 Tyr Tyr NNP cord-022235-6ircruag 28 8 residue residue NN cord-022235-6ircruag 28 9 , , , cord-022235-6ircruag 28 10 its -PRON- PRP$ cord-022235-6ircruag 28 11 progress progress NN cord-022235-6ircruag 28 12 through through IN cord-022235-6ircruag 28 13 the the DT cord-022235-6ircruag 28 14 secretory secretory JJ cord-022235-6ircruag 28 15 pathway pathway NN cord-022235-6ircruag 28 16 , , , cord-022235-6ircruag 28 17 which which WDT cord-022235-6ircruag 28 18 it -PRON- PRP cord-022235-6ircruag 28 19 seems seem VBZ cord-022235-6ircruag 28 20 to to TO cord-022235-6ircruag 28 21 enter enter VB cord-022235-6ircruag 28 22 by by IN cord-022235-6ircruag 28 23 diffusing diffuse VBG cord-022235-6ircruag 28 24 across across IN cord-022235-6ircruag 28 25 the the DT cord-022235-6ircruag 28 26 RERM RERM NNS cord-022235-6ircruag 28 27 , , , cord-022235-6ircruag 28 28 can can MD cord-022235-6ircruag 28 29 be be VB cord-022235-6ircruag 28 30 followed follow VBN cord-022235-6ircruag 28 31 by by IN cord-022235-6ircruag 28 32 chromatography chromatography NN cord-022235-6ircruag 28 33 and and CC cord-022235-6ircruag 28 34 autoradiography autoradiography NN cord-022235-6ircruag 28 35 . . . cord-022235-6ircruag 29 1 Wieland Wieland NNP cord-022235-6ircruag 29 2 et et NNP cord-022235-6ircruag 29 3 al al NNP cord-022235-6ircruag 29 4 . . . cord-022235-6ircruag 30 1 ( ( -LRB- cord-022235-6ircruag 30 2 1190 1190 CD cord-022235-6ircruag 30 3 ) ) -RRB- cord-022235-6ircruag 30 4 found find VBD cord-022235-6ircruag 30 5 that that IN cord-022235-6ircruag 30 6 the the DT cord-022235-6ircruag 30 7 tripeptide tripeptide NN cord-022235-6ircruag 30 8 was be VBD cord-022235-6ircruag 30 9 gly gly RB cord-022235-6ircruag 30 10 cosylated cosylate VBN cord-022235-6ircruag 30 11 in in IN cord-022235-6ircruag 30 12 the the DT cord-022235-6ircruag 30 13 ER ER NNP cord-022235-6ircruag 31 1 ( ( -LRB- cord-022235-6ircruag 31 2 this this DT cord-022235-6ircruag 31 3 prevented prevent VBD cord-022235-6ircruag 31 4 it -PRON- PRP cord-022235-6ircruag 31 5 from from IN cord-022235-6ircruag 31 6 leaking leak VBG cord-022235-6ircruag 31 7 back back RB cord-022235-6ircruag 31 8 into into IN cord-022235-6ircruag 31 9 the the DT cord-022235-6ircruag 31 10 cytosol cytosol NN cord-022235-6ircruag 31 11 ) ) -RRB- cord-022235-6ircruag 31 12 , , , cord-022235-6ircruag 31 13 then then RB cord-022235-6ircruag 31 14 trimmed trim VBN cord-022235-6ircruag 31 15 by by IN cord-022235-6ircruag 31 16 mannosidases mannosidase NNS cord-022235-6ircruag 31 17 located locate VBN cord-022235-6ircruag 31 18 in in IN cord-022235-6ircruag 31 19 the the DT cord-022235-6ircruag 31 20 Golgi Golgi NNP cord-022235-6ircruag 31 21 ( ( -LRB- cord-022235-6ircruag 31 22 Section section NN cord-022235-6ircruag 31 23 V.D V.D NNP cord-022235-6ircruag 31 24 ) ) -RRB- cord-022235-6ircruag 31 25 , , , cord-022235-6ircruag 31 26 and and CC cord-022235-6ircruag 31 27 finally finally RB cord-022235-6ircruag 31 28 secreted secrete VBD cord-022235-6ircruag 31 29 into into IN cord-022235-6ircruag 31 30 the the DT cord-022235-6ircruag 31 31 medium medium NN cord-022235-6ircruag 31 32 . . . cord-022235-6ircruag 32 1 Tripeptide Tripeptide NNP cord-022235-6ircruag 32 2 was be VBD cord-022235-6ircruag 32 3 detected detect VBN cord-022235-6ircruag 32 4 in in IN cord-022235-6ircruag 32 5 the the DT cord-022235-6ircruag 32 6 medium medium NN cord-022235-6ircruag 32 7 after after IN cord-022235-6ircruag 32 8 5 5 CD cord-022235-6ircruag 32 9 - - SYM cord-022235-6ircruag 32 10 10 10 CD cord-022235-6ircruag 32 11 min min NN cord-022235-6ircruag 32 12 , , , cord-022235-6ircruag 32 13 depending depend VBG cord-022235-6ircruag 32 14 on on IN cord-022235-6ircruag 32 15 the the DT cord-022235-6ircruag 32 16 cell cell NN cord-022235-6ircruag 32 17 type type NN cord-022235-6ircruag 32 18 , , , cord-022235-6ircruag 32 19 which which WDT cord-022235-6ircruag 32 20 is be VBZ cord-022235-6ircruag 32 21 considerably considerably RB cord-022235-6ircruag 32 22 faster fast JJR cord-022235-6ircruag 32 23 than than IN cord-022235-6ircruag 32 24 the the DT cord-022235-6ircruag 32 25 time time NN cord-022235-6ircruag 32 26 required require VBN cord-022235-6ircruag 32 27 for for IN cord-022235-6ircruag 32 28 the the DT cord-022235-6ircruag 32 29 transport transport NN cord-022235-6ircruag 32 30 of of IN cord-022235-6ircruag 32 31 most most JJS cord-022235-6ircruag 32 32 secretory secretory JJ cord-022235-6ircruag 32 33 proteins protein NNS cord-022235-6ircruag 32 34 through through IN cord-022235-6ircruag 32 35 the the DT cord-022235-6ircruag 32 36 secretory secretory JJ cord-022235-6ircruag 32 37 pathway pathway NN cord-022235-6ircruag 32 38 . . . cord-022235-6ircruag 33 1 Thus thus RB cord-022235-6ircruag 33 2 , , , cord-022235-6ircruag 33 3 bulk bulk JJ cord-022235-6ircruag 33 4 flow flow NN cord-022235-6ircruag 33 5 is be VBZ cord-022235-6ircruag 33 6 very very RB cord-022235-6ircruag 33 7 efficient efficient JJ cord-022235-6ircruag 33 8 , , , cord-022235-6ircruag 33 9 implying imply VBG cord-022235-6ircruag 33 10 that that IN cord-022235-6ircruag 33 11 diffusion diffusion NN cord-022235-6ircruag 33 12 through through IN cord-022235-6ircruag 33 13 the the DT cord-022235-6ircruag 33 14 lumen lumen NN cord-022235-6ircruag 33 15 of of IN cord-022235-6ircruag 33 16 the the DT cord-022235-6ircruag 33 17 ER ER NNP cord-022235-6ircruag 33 18 and and CC cord-022235-6ircruag 33 19 Golgi Golgi NNP cord-022235-6ircruag 33 20 can can MD cord-022235-6ircruag 33 21 occur occur VB cord-022235-6ircruag 33 22 relatively relatively RB cord-022235-6ircruag 33 23 easily easily RB cord-022235-6ircruag 33 24 and and CC cord-022235-6ircruag 33 25 that that IN cord-022235-6ircruag 33 26 there there EX cord-022235-6ircruag 33 27 is be VBZ cord-022235-6ircruag 33 28 massive massive JJ cord-022235-6ircruag 33 29 , , , cord-022235-6ircruag 33 30 vectorial vectorial JJ cord-022235-6ircruag 33 31 movement movement NN cord-022235-6ircruag 33 32 of of IN cord-022235-6ircruag 33 33 vesicles vesicle NNS cord-022235-6ircruag 33 34 between between IN cord-022235-6ircruag 33 35 the the DT cord-022235-6ircruag 33 36 organelles organelle NNS cord-022235-6ircruag 33 37 and and CC cord-022235-6ircruag 33 38 the the DT cord-022235-6ircruag 33 39 cell cell NN cord-022235-6ircruag 33 40 surface surface NN cord-022235-6ircruag 33 41 . . . cord-022235-6ircruag 34 1 The the DT cord-022235-6ircruag 34 2 way way NN cord-022235-6ircruag 34 3 in in IN cord-022235-6ircruag 34 4 which which WDT cord-022235-6ircruag 34 5 this this DT cord-022235-6ircruag 34 6 result result NN cord-022235-6ircruag 34 7 influences influence VBZ cord-022235-6ircruag 34 8 our -PRON- PRP$ cord-022235-6ircruag 34 9 understanding understanding NN cord-022235-6ircruag 34 10 of of IN cord-022235-6ircruag 34 11 protein protein NN cord-022235-6ircruag 34 12 sorting sorting NN cord-022235-6ircruag 34 13 in in IN cord-022235-6ircruag 34 14 the the DT cord-022235-6ircruag 34 15 secretory secretory JJ cord-022235-6ircruag 34 16 pathway pathway NN cord-022235-6ircruag 34 17 is be VBZ cord-022235-6ircruag 34 18 discussed discuss VBN cord-022235-6ircruag 34 19 in in IN cord-022235-6ircruag 34 20 following follow VBG cord-022235-6ircruag 34 21 sections section NNS cord-022235-6ircruag 34 22 . . . cord-022235-6ircruag 35 1 Soluble soluble JJ cord-022235-6ircruag 35 2 and and CC cord-022235-6ircruag 35 3 integral integral JJ cord-022235-6ircruag 35 4 membrane membrane NN cord-022235-6ircruag 35 5 secretory secretory NN cord-022235-6ircruag 35 6 proteins protein NNS cord-022235-6ircruag 35 7 fold fold VBP cord-022235-6ircruag 35 8 once once RB cord-022235-6ircruag 35 9 they -PRON- PRP cord-022235-6ircruag 35 10 have have VBP cord-022235-6ircruag 35 11 crossed cross VBN cord-022235-6ircruag 35 12 the the DT cord-022235-6ircruag 35 13 RERM RERM NNS cord-022235-6ircruag 35 14 ; ; : cord-022235-6ircruag 35 15 they -PRON- PRP cord-022235-6ircruag 35 16 are be VBP cord-022235-6ircruag 35 17 also also RB cord-022235-6ircruag 35 18 often often RB cord-022235-6ircruag 35 19 covalently covalently RB cord-022235-6ircruag 35 20 modified modify VBN cord-022235-6ircruag 35 21 . . . cord-022235-6ircruag 36 1 The the DT cord-022235-6ircruag 36 2 follow follow NN cord-022235-6ircruag 36 3 ing ing DT cord-022235-6ircruag 36 4 sections section NNS cord-022235-6ircruag 36 5 deal deal VBP cord-022235-6ircruag 36 6 with with IN cord-022235-6ircruag 36 7 the the DT cord-022235-6ircruag 36 8 types type NNS cord-022235-6ircruag 36 9 of of IN cord-022235-6ircruag 36 10 modifications modification NNS cord-022235-6ircruag 36 11 which which WDT cord-022235-6ircruag 36 12 occur occur VBP cord-022235-6ircruag 36 13 in in IN cord-022235-6ircruag 36 14 the the DT cord-022235-6ircruag 36 15 ER er NN cord-022235-6ircruag 36 16 and and CC cord-022235-6ircruag 36 17 their -PRON- PRP$ cord-022235-6ircruag 36 18 role role NN cord-022235-6ircruag 36 19 , , , cord-022235-6ircruag 36 20 if if IN cord-022235-6ircruag 36 21 any any DT cord-022235-6ircruag 36 22 , , , cord-022235-6ircruag 36 23 in in IN cord-022235-6ircruag 36 24 protein protein NN cord-022235-6ircruag 36 25 sorting sorting NN cord-022235-6ircruag 36 26 . . . cord-022235-6ircruag 37 1 Palmitoylation Palmitoylation NNP cord-022235-6ircruag 37 2 and and CC cord-022235-6ircruag 37 3 phosphatidyl phosphatidyl NNP cord-022235-6ircruag 37 4 inositol inositol NN cord-022235-6ircruag 37 5 modification modification NN cord-022235-6ircruag 37 6 of of IN cord-022235-6ircruag 37 7 secretory secretory JJ cord-022235-6ircruag 37 8 proteins protein NNS cord-022235-6ircruag 37 9 are be VBP cord-022235-6ircruag 37 10 discussed discuss VBN cord-022235-6ircruag 37 11 in in IN cord-022235-6ircruag 37 12 Section section NN cord-022235-6ircruag 37 13 III.F.4.C III.F.4.C NNP cord-022235-6ircruag 37 14 ; ; : cord-022235-6ircruag 37 15 only only RB cord-022235-6ircruag 37 16 their -PRON- PRP$ cord-022235-6ircruag 37 17 role role NN cord-022235-6ircruag 37 18 in in IN cord-022235-6ircruag 37 19 protein protein NN cord-022235-6ircruag 37 20 transit transit NN cord-022235-6ircruag 37 21 through through IN cord-022235-6ircruag 37 22 the the DT cord-022235-6ircruag 37 23 secretory secretory JJ cord-022235-6ircruag 37 24 route route NN cord-022235-6ircruag 37 25 will will MD cord-022235-6ircruag 37 26 be be VB cord-022235-6ircruag 37 27 discussed discuss VBN cord-022235-6ircruag 37 28 here here RB cord-022235-6ircruag 37 29 . . . cord-022235-6ircruag 38 1 Most Most JJS cord-022235-6ircruag 38 2 secretory secretory JJ cord-022235-6ircruag 38 3 proteins protein NNS cord-022235-6ircruag 38 4 are be VBP cord-022235-6ircruag 38 5 nitrogen nitrogen NN cord-022235-6ircruag 38 6 ( ( -LRB- cord-022235-6ircruag 38 7 N)-glycosylated N)-glycosylated NNP cord-022235-6ircruag 38 8 in in IN cord-022235-6ircruag 38 9 the the DT cord-022235-6ircruag 38 10 RER rer NN cord-022235-6ircruag 38 11 . . . cord-022235-6ircruag 39 1 Be be VB cord-022235-6ircruag 39 2 cause cause IN cord-022235-6ircruag 39 3 we -PRON- PRP cord-022235-6ircruag 39 4 are be VBP cord-022235-6ircruag 39 5 primarily primarily RB cord-022235-6ircruag 39 6 concerned concern VBN cord-022235-6ircruag 39 7 with with IN cord-022235-6ircruag 39 8 the the DT cord-022235-6ircruag 39 9 effects effect NNS cord-022235-6ircruag 39 10 of of IN cord-022235-6ircruag 39 11 glycosylation glycosylation NN cord-022235-6ircruag 39 12 on on IN cord-022235-6ircruag 39 13 protein protein NN cord-022235-6ircruag 39 14 targeting targeting NN cord-022235-6ircruag 39 15 , , , cord-022235-6ircruag 39 16 only only RB cord-022235-6ircruag 39 17 general general JJ cord-022235-6ircruag 39 18 details detail NNS cord-022235-6ircruag 39 19 of of IN cord-022235-6ircruag 39 20 the the DT cord-022235-6ircruag 39 21 glycosylation glycosylation NN cord-022235-6ircruag 39 22 reactions reaction NNS cord-022235-6ircruag 39 23 will will MD cord-022235-6ircruag 39 24 be be VB cord-022235-6ircruag 39 25 given give VBN cord-022235-6ircruag 39 26 here here RB cord-022235-6ircruag 39 27 . . . cord-022235-6ircruag 40 1 a. a. NN cord-022235-6ircruag 41 1 The the DT cord-022235-6ircruag 41 2 sequence sequence NN cord-022235-6ircruag 41 3 of of IN cord-022235-6ircruag 41 4 reactions reaction NNS cord-022235-6ircruag 41 5 shown show VBN cord-022235-6ircruag 41 6 in in IN cord-022235-6ircruag 41 7 Fig Fig NNP cord-022235-6ircruag 41 8 . . . cord-022235-6ircruag 42 1 V V NNP cord-022235-6ircruag 42 2 .l .l NNP cord-022235-6ircruag 42 3 , , , cord-022235-6ircruag 42 4 which which WDT cord-022235-6ircruag 42 5 is be VBZ cord-022235-6ircruag 42 6 common common JJ cord-022235-6ircruag 42 7 to to IN cord-022235-6ircruag 42 8 both both DT cord-022235-6ircruag 42 9 yeasts yeast NNS cord-022235-6ircruag 42 10 and and CC cord-022235-6ircruag 42 11 higher high JJR cord-022235-6ircruag 42 12 eukaryotes eukaryote NNS cord-022235-6ircruag 42 13 , , , cord-022235-6ircruag 42 14 results result NNS cord-022235-6ircruag 42 15 in in IN cord-022235-6ircruag 42 16 the the DT cord-022235-6ircruag 42 17 addition addition NN cord-022235-6ircruag 42 18 of of IN cord-022235-6ircruag 42 19 a a DT cord-022235-6ircruag 42 20 ( ( -LRB- cord-022235-6ircruag 42 21 Glucose glucose NN cord-022235-6ircruag 42 22 ) ) -RRB- cord-022235-6ircruag 42 23 3 3 CD cord-022235-6ircruag 42 24 -(Mannose -(mannose NN cord-022235-6ircruag 42 25 ) ) -RRB- cord-022235-6ircruag 42 26 9 9 CD cord-022235-6ircruag 42 27 - - : cord-022235-6ircruag 42 28 ( ( -LRB- cord-022235-6ircruag 42 29 J J NNP cord-022235-6ircruag 42 30 /V /V NNP cord-022235-6ircruag 42 31 - - HYPH cord-022235-6ircruag 42 32 acetylglucosamine acetylglucosamine NNP cord-022235-6ircruag 42 33 ) ) -RRB- cord-022235-6ircruag 43 1 2 2 LS cord-022235-6ircruag 43 2 complex complex NN cord-022235-6ircruag 43 3 onto onto IN cord-022235-6ircruag 43 4 Asn Asn NNP cord-022235-6ircruag 43 5 residues residue NNS cord-022235-6ircruag 43 6 in in IN cord-022235-6ircruag 43 7 the the DT cord-022235-6ircruag 43 8 sequence sequence NN cord-022235-6ircruag 43 9 Asn Asn NNP cord-022235-6ircruag 43 10 - - HYPH cord-022235-6ircruag 43 11 X X NNP cord-022235-6ircruag 43 12 - - JJ cord-022235-6ircruag 43 13 Ser ser JJ cord-022235-6ircruag 43 14 or or CC cord-022235-6ircruag 43 15 Thr Thr NNP cord-022235-6ircruag 44 1 ( ( -LRB- cord-022235-6ircruag 44 2 the the DT cord-022235-6ircruag 44 3 acceptor acceptor NN cord-022235-6ircruag 44 4 peptide peptide NN cord-022235-6ircruag 44 5 , , , cord-022235-6ircruag 44 6 in in IN cord-022235-6ircruag 44 7 which which WDT cord-022235-6ircruag 44 8 X x NN cord-022235-6ircruag 44 9 can can MD cord-022235-6ircruag 44 10 be be VB cord-022235-6ircruag 44 11 any any DT cord-022235-6ircruag 44 12 I I NNP cord-022235-6ircruag 44 13 _SP cord-022235-6ircruag 45 1 I -PRON- PRP cord-022235-6ircruag 45 2 _SP cord-022235-6ircruag 46 1 I -PRON- PRP cord-022235-6ircruag 46 2 _SP cord-022235-6ircruag 47 1 I -PRON- PRP cord-022235-6ircruag 47 2 amino amino NNS cord-022235-6ircruag 47 3 acid acid NN cord-022235-6ircruag 47 4 except except IN cord-022235-6ircruag 47 5 possibly possibly RB cord-022235-6ircruag 47 6 proline proline NN cord-022235-6ircruag 47 7 or or CC cord-022235-6ircruag 47 8 aspartate aspartate NN cord-022235-6ircruag 47 9 ) ) -RRB- cord-022235-6ircruag 47 10 . . . cord-022235-6ircruag 48 1 Isolated isolated JJ cord-022235-6ircruag 48 2 tripeptides tripeptide NNS cord-022235-6ircruag 48 3 can can MD cord-022235-6ircruag 48 4 act act VB cord-022235-6ircruag 48 5 as as IN cord-022235-6ircruag 48 6 acceptors acceptor NNS cord-022235-6ircruag 48 7 only only RB cord-022235-6ircruag 48 8 when when WRB cord-022235-6ircruag 48 9 the the DT cord-022235-6ircruag 48 10 two two CD cord-022235-6ircruag 48 11 extremities extremity NNS cord-022235-6ircruag 48 12 are be VBP cord-022235-6ircruag 48 13 blocked block VBN cord-022235-6ircruag 48 14 . . . cord-022235-6ircruag 49 1 Longer long RBR cord-022235-6ircruag 49 2 ac ac IN cord-022235-6ircruag 49 3 ceptor ceptor NN cord-022235-6ircruag 49 4 peptides peptide NNS cord-022235-6ircruag 49 5 are be VBP cord-022235-6ircruag 49 6 more more RBR cord-022235-6ircruag 49 7 rapidly rapidly RB cord-022235-6ircruag 49 8 glycosylated glycosylate VBN cord-022235-6ircruag 49 9 ( ( -LRB- cord-022235-6ircruag 49 10 577 577 CD cord-022235-6ircruag 49 11 ) ) -RRB- cord-022235-6ircruag 49 12 . . . cord-022235-6ircruag 50 1 As as IN cord-022235-6ircruag 50 2 shown show VBN cord-022235-6ircruag 50 3 in in IN cord-022235-6ircruag 50 4 Fig Fig NNP cord-022235-6ircruag 50 5 . . . cord-022235-6ircruag 50 6 _SP cord-022235-6ircruag 51 1 V v LS cord-022235-6ircruag 51 2 .l .l . cord-022235-6ircruag 52 1 , , , cord-022235-6ircruag 52 2 the the DT cord-022235-6ircruag 52 3 entire entire JJ cord-022235-6ircruag 52 4 complex complex NN cord-022235-6ircruag 52 5 is be VBZ cord-022235-6ircruag 52 6 assembled assemble VBN cord-022235-6ircruag 52 7 before before IN cord-022235-6ircruag 52 8 it -PRON- PRP cord-022235-6ircruag 52 9 is be VBZ cord-022235-6ircruag 52 10 attached attach VBN cord-022235-6ircruag 52 11 to to IN cord-022235-6ircruag 52 12 the the DT cord-022235-6ircruag 52 13 Asn Asn NNP cord-022235-6ircruag 52 14 residue residue NN cord-022235-6ircruag 52 15 in in IN cord-022235-6ircruag 52 16 the the DT cord-022235-6ircruag 52 17 lumen lumen NN cord-022235-6ircruag 52 18 of of IN cord-022235-6ircruag 52 19 the the DT cord-022235-6ircruag 52 20 RER RER NNP cord-022235-6ircruag 52 21 . . . cord-022235-6ircruag 53 1 At at IN cord-022235-6ircruag 53 2 least least JJS cord-022235-6ircruag 53 3 some some DT cord-022235-6ircruag 53 4 sugars sugar NNS cord-022235-6ircruag 53 5 cross cross VBP cord-022235-6ircruag 53 6 the the DT cord-022235-6ircruag 53 7 RERM RERM NNP cord-022235-6ircruag 53 8 as as IN cord-022235-6ircruag 53 9 intermediates intermediate NNS cord-022235-6ircruag 53 10 complexed complexe VBN cord-022235-6ircruag 53 11 with with IN cord-022235-6ircruag 53 12 the the DT cord-022235-6ircruag 53 13 long long JJ cord-022235-6ircruag 53 14 - - HYPH cord-022235-6ircruag 53 15 chain chain NN cord-022235-6ircruag 53 16 lipid lipid NN cord-022235-6ircruag 53 17 dolichol dolichol NN cord-022235-6ircruag 53 18 , , , cord-022235-6ircruag 53 19 whereas whereas IN cord-022235-6ircruag 53 20 others other NNS cord-022235-6ircruag 53 21 may may MD cord-022235-6ircruag 53 22 cross cross VB cord-022235-6ircruag 53 23 the the DT cord-022235-6ircruag 53 24 RERM RERM NNP cord-022235-6ircruag 53 25 as as IN cord-022235-6ircruag 53 26 nucleotide nucleotide JJ cord-022235-6ircruag 53 27 5 5 CD cord-022235-6ircruag 53 28 ' ' POS cord-022235-6ircruag 53 29 -diphosphate -diphosphate CD cord-022235-6ircruag 53 30 - - HYPH cord-022235-6ircruag 53 31 sugar sugar NN cord-022235-6ircruag 53 32 complexes complex NNS cord-022235-6ircruag 53 33 . . . cord-022235-6ircruag 54 1 However however RB cord-022235-6ircruag 54 2 , , , cord-022235-6ircruag 54 3 it -PRON- PRP cord-022235-6ircruag 54 4 is be VBZ cord-022235-6ircruag 54 5 by by IN cord-022235-6ircruag 54 6 no no DT cord-022235-6ircruag 54 7 means mean NNS cord-022235-6ircruag 54 8 certain certain JJ cord-022235-6ircruag 54 9 that that IN cord-022235-6ircruag 54 10 the the DT cord-022235-6ircruag 54 11 complex complex NN cord-022235-6ircruag 54 12 is be VBZ cord-022235-6ircruag 54 13 assembled assemble VBN cord-022235-6ircruag 54 14 entirely entirely RB cord-022235-6ircruag 54 15 within within IN cord-022235-6ircruag 54 16 the the DT cord-022235-6ircruag 54 17 lumen lumen NN cord-022235-6ircruag 54 18 of of IN cord-022235-6ircruag 54 19 the the DT cord-022235-6ircruag 54 20 RER RER NNP cord-022235-6ircruag 54 21 . . . cord-022235-6ircruag 55 1 For for IN cord-022235-6ircruag 55 2 example example NN cord-022235-6ircruag 55 3 , , , cord-022235-6ircruag 55 4 there there EX cord-022235-6ircruag 55 5 is be VBZ cord-022235-6ircruag 55 6 no no DT cord-022235-6ircruag 55 7 evidence evidence NN cord-022235-6ircruag 55 8 that that IN cord-022235-6ircruag 55 9 GDP gdp NN cord-022235-6ircruag 55 10 - - HYPH cord-022235-6ircruag 55 11 mannose mannose NN cord-022235-6ircruag 55 12 can can MD cord-022235-6ircruag 55 13 be be VB cord-022235-6ircruag 55 14 trans trans NNP cord-022235-6ircruag 55 15 ported port VBN cord-022235-6ircruag 55 16 across across IN cord-022235-6ircruag 55 17 the the DT cord-022235-6ircruag 55 18 RERM RERM NNP cord-022235-6ircruag 55 19 , , , cord-022235-6ircruag 55 20 leading lead VBG cord-022235-6ircruag 55 21 to to IN cord-022235-6ircruag 55 22 the the DT cord-022235-6ircruag 55 23 proposal proposal NN cord-022235-6ircruag 55 24 that that IN cord-022235-6ircruag 55 25 the the DT cord-022235-6ircruag 55 26 five five CD cord-022235-6ircruag 55 27 mannose mannose NN cord-022235-6ircruag 55 28 residues residue NNS cord-022235-6ircruag 55 29 are be VBP cord-022235-6ircruag 55 30 added add VBN cord-022235-6ircruag 55 31 via via IN cord-022235-6ircruag 55 32 GDP gdp NN cord-022235-6ircruag 55 33 - - HYPH cord-022235-6ircruag 55 34 mannose mannose VBG cord-022235-6ircruag 55 35 donors donor NNS cord-022235-6ircruag 55 36 on on IN cord-022235-6ircruag 55 37 the the DT cord-022235-6ircruag 55 38 cytoplasmic cytoplasmic JJ cord-022235-6ircruag 55 39 face face NN cord-022235-6ircruag 55 40 of of IN cord-022235-6ircruag 55 41 the the DT cord-022235-6ircruag 55 42 RERM RERM NNS cord-022235-6ircruag 55 43 , , , cord-022235-6ircruag 55 44 whereas whereas IN cord-022235-6ircruag 55 45 later later JJ cord-022235-6ircruag 55 46 modifications modification NNS cord-022235-6ircruag 55 47 , , , cord-022235-6ircruag 55 48 and and CC cord-022235-6ircruag 55 49 possibly possibly RB cord-022235-6ircruag 55 50 earlier early RBR cord-022235-6ircruag 55 51 modifica modifica NNP cord-022235-6ircruag 55 52 tions tion NNS cord-022235-6ircruag 55 53 , , , cord-022235-6ircruag 55 54 occur occur VBP cord-022235-6ircruag 55 55 in in IN cord-022235-6ircruag 55 56 the the DT cord-022235-6ircruag 55 57 lumen lumen NN cord-022235-6ircruag 55 58 . . . cord-022235-6ircruag 56 1 This this DT cord-022235-6ircruag 56 2 presupposes presuppose VBZ cord-022235-6ircruag 56 3 that that IN cord-022235-6ircruag 56 4 the the DT cord-022235-6ircruag 56 5 dolichol dolichol NNP cord-022235-6ircruag 56 6 - - HYPH cord-022235-6ircruag 56 7 PP-(NAGn pp-(nagn NN cord-022235-6ircruag 56 8 ) ) -RRB- cord-022235-6ircruag 56 9 2 2 CD cord-022235-6ircruag 56 10 complex complex NN cord-022235-6ircruag 56 11 can can MD cord-022235-6ircruag 56 12 " " `` cord-022235-6ircruag 56 13 flip flip VB cord-022235-6ircruag 56 14 " " '' cord-022235-6ircruag 56 15 from from IN cord-022235-6ircruag 56 16 the the DT cord-022235-6ircruag 56 17 lumen lumen NN cord-022235-6ircruag 56 18 to to IN cord-022235-6ircruag 56 19 the the DT cord-022235-6ircruag 56 20 cytoplasmic cytoplasmic JJ cord-022235-6ircruag 56 21 face face NN cord-022235-6ircruag 56 22 and and CC cord-022235-6ircruag 56 23 then then RB cord-022235-6ircruag 56 24 back back RB cord-022235-6ircruag 56 25 again again RB cord-022235-6ircruag 56 26 once once IN cord-022235-6ircruag 56 27 the the DT cord-022235-6ircruag 56 28 five five CD cord-022235-6ircruag 56 29 mannose mannose NN cord-022235-6ircruag 56 30 residues residue NNS cord-022235-6ircruag 56 31 have have VBP cord-022235-6ircruag 56 32 been be VBN cord-022235-6ircruag 56 33 added add VBN cord-022235-6ircruag 56 34 [ [ -LRB- cord-022235-6ircruag 56 35 see see VB cord-022235-6ircruag 56 36 ( ( -LRB- cord-022235-6ircruag 56 37 452 452 CD cord-022235-6ircruag 56 38 ) ) -RRB- cord-022235-6ircruag 56 39 for for IN cord-022235-6ircruag 56 40 further further JJ cord-022235-6ircruag 56 41 discussion discussion NN cord-022235-6ircruag 56 42 of of IN cord-022235-6ircruag 56 43 the the DT cord-022235-6ircruag 56 44 topology topology NN cord-022235-6ircruag 56 45 of of IN cord-022235-6ircruag 56 46 glycosylation glycosylation NN cord-022235-6ircruag 56 47 reactions reaction NNS cord-022235-6ircruag 56 48 in in IN cord-022235-6ircruag 56 49 the the DT cord-022235-6ircruag 56 50 RER RER NNP cord-022235-6ircruag 56 51 ] ] -RRB- cord-022235-6ircruag 56 52 . . . cord-022235-6ircruag 57 1 The the DT cord-022235-6ircruag 57 2 preferred preferred JJ cord-022235-6ircruag 57 3 donor donor NN cord-022235-6ircruag 57 4 lipid lipid NN cord-022235-6ircruag 57 5 - - HYPH cord-022235-6ircruag 57 6 carbohydrate carbohydrate NN cord-022235-6ircruag 57 7 complex complex NN cord-022235-6ircruag 57 8 is be VBZ cord-022235-6ircruag 57 9 the the DT cord-022235-6ircruag 57 10 com com NN cord-022235-6ircruag 57 11 plete plete NN cord-022235-6ircruag 57 12 complex complex NN cord-022235-6ircruag 57 13 shown show VBN cord-022235-6ircruag 57 14 in in IN cord-022235-6ircruag 57 15 Fig Fig NNP cord-022235-6ircruag 57 16 . . . cord-022235-6ircruag 57 17 V.l V.l NNP cord-022235-6ircruag 57 18 ( ( -LRB- cord-022235-6ircruag 57 19 1129 1129 CD cord-022235-6ircruag 57 20 ) ) -RRB- cord-022235-6ircruag 57 21 . . . cord-022235-6ircruag 58 1 However however RB cord-022235-6ircruag 58 2 , , , cord-022235-6ircruag 58 3 truncated truncated JJ cord-022235-6ircruag 58 4 versions version NNS cord-022235-6ircruag 58 5 lacking lack VBG cord-022235-6ircruag 58 6 glucose glucose NN cord-022235-6ircruag 58 7 and and CC cord-022235-6ircruag 58 8 even even RB cord-022235-6ircruag 58 9 mannose mannose NN cord-022235-6ircruag 58 10 residues residue NNS cord-022235-6ircruag 58 11 can can MD cord-022235-6ircruag 58 12 act act VB cord-022235-6ircruag 58 13 as as IN cord-022235-6ircruag 58 14 donors donor NNS cord-022235-6ircruag 58 15 in in IN cord-022235-6ircruag 58 16 vitro vitro FW cord-022235-6ircruag 58 17 , , , cord-022235-6ircruag 58 18 and and CC cord-022235-6ircruag 58 19 under under IN cord-022235-6ircruag 58 20 - - HYPH cord-022235-6ircruag 58 21 glycosylated glycosylate VBN cord-022235-6ircruag 58 22 complexes complex NNS cord-022235-6ircruag 58 23 can can MD cord-022235-6ircruag 58 24 act act VB cord-022235-6ircruag 58 25 as as IN cord-022235-6ircruag 58 26 donors donor NNS cord-022235-6ircruag 58 27 in in IN cord-022235-6ircruag 58 28 vivo vivo NN cord-022235-6ircruag 58 29 in in IN cord-022235-6ircruag 58 30 yeasts yeast NNS cord-022235-6ircruag 58 31 ( ( -LRB- cord-022235-6ircruag 58 32 598 598 CD cord-022235-6ircruag 58 33 ) ) -RRB- cord-022235-6ircruag 58 34 and and CC cord-022235-6ircruag 58 35 protozoa protozoan NNS cord-022235-6ircruag 58 36 ( ( -LRB- cord-022235-6ircruag 58 37 577 577 CD cord-022235-6ircruag 58 38 ) ) -RRB- cord-022235-6ircruag 58 39 . . . cord-022235-6ircruag 59 1 Variations variation NNS cord-022235-6ircruag 59 2 in in IN cord-022235-6ircruag 59 3 the the DT cord-022235-6ircruag 59 4 mannose mannose NN cord-022235-6ircruag 59 5 content content NN cord-022235-6ircruag 59 6 of of IN cord-022235-6ircruag 59 7 transferred transfer VBN cord-022235-6ircruag 59 8 oligosaccharides oligosaccharide NNS cord-022235-6ircruag 59 9 have have VBP cord-022235-6ircruag 59 10 also also RB cord-022235-6ircruag 59 11 been be VBN cord-022235-6ircruag 59 12 reported report VBN cord-022235-6ircruag 59 13 ( ( -LRB- cord-022235-6ircruag 59 14 577 577 CD cord-022235-6ircruag 59 15 ) ) -RRB- cord-022235-6ircruag 59 16 . . . cord-022235-6ircruag 60 1 The the DT cord-022235-6ircruag 60 2 transfer transfer NN cord-022235-6ircruag 60 3 of of IN cord-022235-6ircruag 60 4 the the DT cord-022235-6ircruag 60 5 first first JJ cord-022235-6ircruag 60 6 N N NNP cord-022235-6ircruag 60 7 - - HYPH cord-022235-6ircruag 60 8 acetylglucosamine acetylglucosamine NNP cord-022235-6ircruag 60 9 ( ( -LRB- cord-022235-6ircruag 60 10 NAGn nagn NN cord-022235-6ircruag 60 11 ) ) -RRB- cord-022235-6ircruag 60 12 residue residue NN cord-022235-6ircruag 60 13 from from IN cord-022235-6ircruag 60 14 the the DT cord-022235-6ircruag 60 15 UDP udp NN cord-022235-6ircruag 60 16 complex complex NN cord-022235-6ircruag 60 17 to to IN cord-022235-6ircruag 60 18 the the DT cord-022235-6ircruag 60 19 dolichol dolichol NN cord-022235-6ircruag 60 20 , , , cord-022235-6ircruag 60 21 is be VBZ cord-022235-6ircruag 60 22 inhibited inhibit VBN cord-022235-6ircruag 60 23 by by IN cord-022235-6ircruag 60 24 the the DT cord-022235-6ircruag 60 25 antibiotic antibiotic JJ cord-022235-6ircruag 60 26 tunicamycin tunicamycin NNS cord-022235-6ircruag 60 27 , , , cord-022235-6ircruag 60 28 which which WDT cord-022235-6ircruag 60 29 therefore therefore RB cord-022235-6ircruag 60 30 blocks block VBZ cord-022235-6ircruag 60 31 all all DT cord-022235-6ircruag 60 32 N n NN cord-022235-6ircruag 60 33 - - HYPH cord-022235-6ircruag 60 34 glycosylation glycosylation NN cord-022235-6ircruag 60 35 . . . cord-022235-6ircruag 61 1 This this DT cord-022235-6ircruag 61 2 provides provide VBZ cord-022235-6ircruag 61 3 a a DT cord-022235-6ircruag 61 4 valuable valuable JJ cord-022235-6ircruag 61 5 tech tech NN cord-022235-6ircruag 61 6 nique nique NN cord-022235-6ircruag 61 7 for for IN cord-022235-6ircruag 61 8 studying study VBG cord-022235-6ircruag 61 9 the the DT cord-022235-6ircruag 61 10 role role NN cord-022235-6ircruag 61 11 of of IN cord-022235-6ircruag 61 12 N n NN cord-022235-6ircruag 61 13 - - HYPH cord-022235-6ircruag 61 14 glycosylation glycosylation NN cord-022235-6ircruag 61 15 in in IN cord-022235-6ircruag 61 16 protein protein NN cord-022235-6ircruag 61 17 traffic traffic NN cord-022235-6ircruag 61 18 . . . cord-022235-6ircruag 62 1 The the DT cord-022235-6ircruag 62 2 yeast yeast NN cord-022235-6ircruag 62 3 ( ( -LRB- cord-022235-6ircruag 62 4 S. S. NNP cord-022235-6ircruag 62 5 cerevisiae cerevisiae NN cord-022235-6ircruag 62 6 ) ) -RRB- cord-022235-6ircruag 62 7 gene gene NN cord-022235-6ircruag 62 8 ( ( -LRB- cord-022235-6ircruag 62 9 ALG7 ALG7 NNP cord-022235-6ircruag 62 10 ) ) -RRB- cord-022235-6ircruag 62 11 coding code VBG cord-022235-6ircruag 62 12 for for IN cord-022235-6ircruag 62 13 the the DT cord-022235-6ircruag 62 14 RERM RERM NNP cord-022235-6ircruag 62 15 - - HYPH cord-022235-6ircruag 62 16 associated associate VBN cord-022235-6ircruag 62 17 , , , cord-022235-6ircruag 62 18 tunicamycin tunicamycin NNS cord-022235-6ircruag 62 19 - - HYPH cord-022235-6ircruag 62 20 sensitive sensitive JJ cord-022235-6ircruag 62 21 enzyme enzyme NN cord-022235-6ircruag 63 1 ( ( -LRB- cord-022235-6ircruag 63 2 UDP UDP NNP cord-022235-6ircruag 63 3 - - HYPH cord-022235-6ircruag 63 4 NAGn NAGn NNP cord-022235-6ircruag 63 5 : : : cord-022235-6ircruag 63 6 dolichol dolichol NNP cord-022235-6ircruag 63 7 - - HYPH cord-022235-6ircruag 63 8 P p NN cord-022235-6ircruag 63 9 - - HYPH cord-022235-6ircruag 63 10 transferase transferase NN cord-022235-6ircruag 63 11 ) ) -RRB- cord-022235-6ircruag 63 12 was be VBD cord-022235-6ircruag 63 13 cloned clone VBN cord-022235-6ircruag 63 14 by by IN cord-022235-6ircruag 63 15 virtue virtue NN cord-022235-6ircruag 63 16 of of IN cord-022235-6ircruag 63 17 its -PRON- PRP$ cord-022235-6ircruag 63 18 ability ability NN cord-022235-6ircruag 63 19 to to TO cord-022235-6ircruag 63 20 rescue rescue VB cord-022235-6ircruag 63 21 tunicamycin tunicamycin NNS cord-022235-6ircruag 63 22 - - HYPH cord-022235-6ircruag 63 23 treated treat VBN cord-022235-6ircruag 63 24 cells cell NNS cord-022235-6ircruag 63 25 when when WRB cord-022235-6ircruag 63 26 present present JJ cord-022235-6ircruag 63 27 on on IN cord-022235-6ircruag 63 28 multiple multiple JJ cord-022235-6ircruag 63 29 copy copy NN cord-022235-6ircruag 63 30 number number NN cord-022235-6ircruag 63 31 plasmids plasmid NNS cord-022235-6ircruag 63 32 ( ( -LRB- cord-022235-6ircruag 63 33 931 931 CD cord-022235-6ircruag 63 34 ) ) -RRB- cord-022235-6ircruag 63 35 . . . cord-022235-6ircruag 64 1 Null Null NNP cord-022235-6ircruag 64 2 mutations mutation NNS cord-022235-6ircruag 64 3 in in IN cord-022235-6ircruag 64 4 ALG7 ALG7 NNP cord-022235-6ircruag 64 5 are be VBP cord-022235-6ircruag 64 6 lethal lethal JJ cord-022235-6ircruag 64 7 . . . cord-022235-6ircruag 65 1 Mutants mutant NNS cord-022235-6ircruag 65 2 affected affect VBN cord-022235-6ircruag 65 3 in in IN cord-022235-6ircruag 65 4 other other JJ cord-022235-6ircruag 65 5 stages stage NNS cord-022235-6ircruag 65 6 in in IN cord-022235-6ircruag 65 7 the the DT cord-022235-6ircruag 65 8 yeast yeast NN cord-022235-6ircruag 65 9 glycosylation glycosylation NN cord-022235-6ircruag 65 10 pathway pathway NN cord-022235-6ircruag 65 11 have have VBP cord-022235-6ircruag 65 12 been be VBN cord-022235-6ircruag 65 13 isolated isolate VBN cord-022235-6ircruag 65 14 by by IN cord-022235-6ircruag 65 15 mannose mannose NN cord-022235-6ircruag 65 16 suicide suicide NN cord-022235-6ircruag 65 17 selection selection NN cord-022235-6ircruag 65 18 ( ( -LRB- cord-022235-6ircruag 65 19 Section section NN cord-022235-6ircruag 65 20 II.F.l.b II.F.l.b NNS cord-022235-6ircruag 65 21 ) ) -RRB- cord-022235-6ircruag 65 22 . . . cord-022235-6ircruag 66 1 Only only RB cord-022235-6ircruag 66 2 muta muta NN cord-022235-6ircruag 66 3 tions tion NNS cord-022235-6ircruag 66 4 blocking block VBG cord-022235-6ircruag 66 5 the the DT cord-022235-6ircruag 66 6 earliest early JJS cord-022235-6ircruag 66 7 stages stage NNS cord-022235-6ircruag 66 8 in in IN cord-022235-6ircruag 66 9 the the DT cord-022235-6ircruag 66 10 pathway pathway NN cord-022235-6ircruag 66 11 are be VBP cord-022235-6ircruag 66 12 lethal lethal JJ cord-022235-6ircruag 66 13 ; ; : cord-022235-6ircruag 66 14 incomplete incomplete JJ cord-022235-6ircruag 66 15 dolichol dolichol NN cord-022235-6ircruag 66 16 - - HYPH cord-022235-6ircruag 66 17 linked link VBN cord-022235-6ircruag 66 18 oligosaccharides oligosaccharide NNS cord-022235-6ircruag 66 19 containing contain VBG cord-022235-6ircruag 66 20 a a DT cord-022235-6ircruag 66 21 minimum minimum NN cord-022235-6ircruag 66 22 of of IN cord-022235-6ircruag 66 23 four four CD cord-022235-6ircruag 66 24 mannose mannose NN cord-022235-6ircruag 66 25 residues residue NNS cord-022235-6ircruag 66 26 allow allow VBP cord-022235-6ircruag 66 27 normal normal JJ cord-022235-6ircruag 66 28 growth growth NN cord-022235-6ircruag 66 29 , , , cord-022235-6ircruag 66 30 presumably presumably RB cord-022235-6ircruag 66 31 because because IN cord-022235-6ircruag 66 32 they -PRON- PRP cord-022235-6ircruag 66 33 can can MD cord-022235-6ircruag 66 34 be be VB cord-022235-6ircruag 66 35 attached attach VBN cord-022235-6ircruag 66 36 to to IN cord-022235-6ircruag 66 37 secretory secretory JJ cord-022235-6ircruag 66 38 proteins protein NNS cord-022235-6ircruag 66 39 if if IN cord-022235-6ircruag 66 40 the the DT cord-022235-6ircruag 66 41 full full JJ cord-022235-6ircruag 66 42 - - HYPH cord-022235-6ircruag 66 43 length length NN cord-022235-6ircruag 66 44 lipid lipid NN cord-022235-6ircruag 66 45 - - HYPH cord-022235-6ircruag 66 46 oligosaccharide oligosaccharide NN cord-022235-6ircruag 66 47 complex complex NN cord-022235-6ircruag 66 48 is be VBZ cord-022235-6ircruag 66 49 absent absent JJ cord-022235-6ircruag 66 50 ( ( -LRB- cord-022235-6ircruag 66 51 see see VB cord-022235-6ircruag 66 52 above above RB cord-022235-6ircruag 66 53 ) ) -RRB- cord-022235-6ircruag 66 54 ( ( -LRB- cord-022235-6ircruag 66 55 598 598 CD cord-022235-6ircruag 66 56 ) ) -RRB- cord-022235-6ircruag 66 57 . . . cord-022235-6ircruag 67 1 The the DT cord-022235-6ircruag 67 2 complete complete JJ cord-022235-6ircruag 67 3 oligosaccharide oligosaccharide NN cord-022235-6ircruag 67 4 chain chain NN cord-022235-6ircruag 67 5 is be VBZ cord-022235-6ircruag 67 6 transferred transfer VBN cord-022235-6ircruag 67 7 onto onto IN cord-022235-6ircruag 67 8 the the DT cord-022235-6ircruag 67 9 Asn Asn NNP cord-022235-6ircruag 67 10 accep accep XX cord-022235-6ircruag 67 11 tor tor NN cord-022235-6ircruag 67 12 site site NN cord-022235-6ircruag 67 13 in in IN cord-022235-6ircruag 67 14 the the DT cord-022235-6ircruag 67 15 lumen lumen NN cord-022235-6ircruag 67 16 of of IN cord-022235-6ircruag 67 17 the the DT cord-022235-6ircruag 67 18 RER RER NNP cord-022235-6ircruag 67 19 . . . cord-022235-6ircruag 68 1 N n NN cord-022235-6ircruag 68 2 - - HYPH cord-022235-6ircruag 68 3 glycosylation glycosylation NN cord-022235-6ircruag 68 4 is be VBZ cord-022235-6ircruag 68 5 generally generally RB cord-022235-6ircruag 68 6 thought think VBN cord-022235-6ircruag 68 7 to to TO cord-022235-6ircruag 68 8 occur occur VB cord-022235-6ircruag 68 9 as as IN cord-022235-6ircruag 68 10 the the DT cord-022235-6ircruag 68 11 polypeptide polypeptide NN cord-022235-6ircruag 68 12 is be VBZ cord-022235-6ircruag 68 13 being be VBG cord-022235-6ircruag 68 14 threaded thread VBN cord-022235-6ircruag 68 15 through through IN cord-022235-6ircruag 68 16 the the DT cord-022235-6ircruag 68 17 RERM RERM NNP cord-022235-6ircruag 68 18 , , , cord-022235-6ircruag 68 19 while while IN cord-022235-6ircruag 68 20 it -PRON- PRP cord-022235-6ircruag 68 21 is be VBZ cord-022235-6ircruag 68 22 still still RB cord-022235-6ircruag 68 23 in in IN cord-022235-6ircruag 68 24 its -PRON- PRP$ cord-022235-6ircruag 68 25 unfolded unfolded JJ cord-022235-6ircruag 68 26 state state NN cord-022235-6ircruag 68 27 . . . cord-022235-6ircruag 69 1 However however RB cord-022235-6ircruag 69 2 , , , cord-022235-6ircruag 69 3 some some DT cord-022235-6ircruag 69 4 acceptor acceptor NN cord-022235-6ircruag 69 5 sites site NNS cord-022235-6ircruag 69 6 are be VBP cord-022235-6ircruag 69 7 not not RB cord-022235-6ircruag 69 8 glyco glyco NNP cord-022235-6ircruag 69 9 - - HYPH cord-022235-6ircruag 69 10 sylated sylated NNP cord-022235-6ircruag 69 11 . . . cord-022235-6ircruag 70 1 This this DT cord-022235-6ircruag 70 2 may may MD cord-022235-6ircruag 70 3 be be VB cord-022235-6ircruag 70 4 because because IN cord-022235-6ircruag 70 5 they -PRON- PRP cord-022235-6ircruag 70 6 rapidly rapidly RB cord-022235-6ircruag 70 7 become become VBP cord-022235-6ircruag 70 8 inaccessible inaccessible JJ cord-022235-6ircruag 70 9 within within IN cord-022235-6ircruag 70 10 the the DT cord-022235-6ircruag 70 11 structure structure NN cord-022235-6ircruag 70 12 of of IN cord-022235-6ircruag 70 13 the the DT cord-022235-6ircruag 70 14 folded fold VBN cord-022235-6ircruag 70 15 polypeptide polypeptide NN cord-022235-6ircruag 70 16 , , , cord-022235-6ircruag 70 17 although although IN cord-022235-6ircruag 70 18 other other JJ cord-022235-6ircruag 70 19 explanations explanation NNS cord-022235-6ircruag 70 20 are be VBP cord-022235-6ircruag 70 21 possi possi FW cord-022235-6ircruag 70 22 ble ble NN cord-022235-6ircruag 70 23 ( ( -LRB- cord-022235-6ircruag 70 24 577 577 CD cord-022235-6ircruag 70 25 , , , cord-022235-6ircruag 70 26 968 968 CD cord-022235-6ircruag 70 27 ) ) -RRB- cord-022235-6ircruag 70 28 . . . cord-022235-6ircruag 71 1 An an DT cord-022235-6ircruag 71 2 unexplained unexplained JJ cord-022235-6ircruag 71 3 anomaly anomaly NN cord-022235-6ircruag 71 4 is be VBZ cord-022235-6ircruag 71 5 that that IN cord-022235-6ircruag 71 6 some some DT cord-022235-6ircruag 71 7 ER ER NNP cord-022235-6ircruag 71 8 membrane membrane NN cord-022235-6ircruag 71 9 glyco glyco NNS cord-022235-6ircruag 71 10 proteins protein NNS cord-022235-6ircruag 71 11 have have VBP cord-022235-6ircruag 71 12 glycosylated glycosylate VBN cord-022235-6ircruag 71 13 residues residue NNS cord-022235-6ircruag 71 14 exposed expose VBN cord-022235-6ircruag 71 15 on on IN cord-022235-6ircruag 71 16 the the DT cord-022235-6ircruag 71 17 cytoplasmic cytoplasmic JJ cord-022235-6ircruag 71 18 face face NN cord-022235-6ircruag 71 19 of of IN cord-022235-6ircruag 71 20 the the DT cord-022235-6ircruag 71 21 ER ER NNP cord-022235-6ircruag 71 22 ( ( -LRB- cord-022235-6ircruag 71 23 1 1 CD cord-022235-6ircruag 71 24 ) ) -RRB- cord-022235-6ircruag 71 25 . . . cord-022235-6ircruag 72 1 One one CD cord-022235-6ircruag 72 2 explanation explanation NN cord-022235-6ircruag 72 3 could could MD cord-022235-6ircruag 72 4 be be VB cord-022235-6ircruag 72 5 that that IN cord-022235-6ircruag 72 6 additional additional JJ cord-022235-6ircruag 72 7 NAGn nagn NN cord-022235-6ircruag 72 8 transferases transferase NNS cord-022235-6ircruag 72 9 are be VBP cord-022235-6ircruag 72 10 present present JJ cord-022235-6ircruag 72 11 on on IN cord-022235-6ircruag 72 12 the the DT cord-022235-6ircruag 72 13 cytoplasmic cytoplasmic JJ cord-022235-6ircruag 72 14 face face NN cord-022235-6ircruag 72 15 of of IN cord-022235-6ircruag 72 16 the the DT cord-022235-6ircruag 72 17 smooth smooth JJ cord-022235-6ircruag 72 18 ER er NN cord-022235-6ircruag 72 19 , , , cord-022235-6ircruag 72 20 where where WRB cord-022235-6ircruag 72 21 these these DT cord-022235-6ircruag 72 22 glyco glyco NNS cord-022235-6ircruag 72 23 proteins protein NNS cord-022235-6ircruag 72 24 are be VBP cord-022235-6ircruag 72 25 predominantly predominantly RB cord-022235-6ircruag 72 26 located locate VBN cord-022235-6ircruag 72 27 . . . cord-022235-6ircruag 73 1 The the DT cord-022235-6ircruag 73 2 initially initially RB cord-022235-6ircruag 73 3 homogeneous homogeneous JJ cord-022235-6ircruag 73 4 oligosaccharide oligosaccharide NN cord-022235-6ircruag 73 5 is be VBZ cord-022235-6ircruag 73 6 processed process VBN cord-022235-6ircruag 73 7 immediately immediately RB cord-022235-6ircruag 73 8 fol fol NNP cord-022235-6ircruag 73 9 lowing low VBG cord-022235-6ircruag 73 10 its -PRON- PRP$ cord-022235-6ircruag 73 11 attachment attachment NN cord-022235-6ircruag 73 12 to to IN cord-022235-6ircruag 73 13 the the DT cord-022235-6ircruag 73 14 polypeptide polypeptide NN cord-022235-6ircruag 73 15 chain chain NN cord-022235-6ircruag 73 16 , , , cord-022235-6ircruag 73 17 initially initially RB cord-022235-6ircruag 73 18 by by IN cord-022235-6ircruag 73 19 the the DT cord-022235-6ircruag 73 20 removal removal NN cord-022235-6ircruag 73 21 of of IN cord-022235-6ircruag 73 22 glucose glucose NN cord-022235-6ircruag 73 23 residues residue NNS cord-022235-6ircruag 73 24 by by IN cord-022235-6ircruag 73 25 one one CD cord-022235-6ircruag 73 26 or or CC cord-022235-6ircruag 73 27 more more JJR cord-022235-6ircruag 73 28 glucosidases glucosidase NNS cord-022235-6ircruag 73 29 . . . cord-022235-6ircruag 74 1 Glucose glucose NN cord-022235-6ircruag 74 2 trimming trimming NN cord-022235-6ircruag 74 3 of of IN cord-022235-6ircruag 74 4 glyco glyco NNP cord-022235-6ircruag 74 5 sylated sylated NNP cord-022235-6ircruag 74 6 vesicular vesicular JJ cord-022235-6ircruag 74 7 stomatitis stomatitis NN cord-022235-6ircruag 74 8 virus virus NN cord-022235-6ircruag 74 9 ( ( -LRB- cord-022235-6ircruag 74 10 VSV VSV NNP cord-022235-6ircruag 74 11 ) ) -RRB- cord-022235-6ircruag 75 1 G g NN cord-022235-6ircruag 75 2 protein protein NN cord-022235-6ircruag 75 3 has have VBZ cord-022235-6ircruag 75 4 been be VBN cord-022235-6ircruag 75 5 reported report VBN cord-022235-6ircruag 75 6 to to TO cord-022235-6ircruag 75 7 occur occur VB cord-022235-6ircruag 75 8 cotranslationally cotranslationally RB cord-022235-6ircruag 75 9 ( ( -LRB- cord-022235-6ircruag 75 10 27 27 CD cord-022235-6ircruag 75 11 ) ) -RRB- cord-022235-6ircruag 75 12 . . . cord-022235-6ircruag 76 1 Further further JJ cord-022235-6ircruag 76 2 processing processing NN cord-022235-6ircruag 76 3 steps step NNS cord-022235-6ircruag 76 4 are be VBP cord-022235-6ircruag 76 5 different different JJ cord-022235-6ircruag 76 6 in in IN cord-022235-6ircruag 76 7 yeasts yeast NNS cord-022235-6ircruag 76 8 and and CC cord-022235-6ircruag 76 9 in in IN cord-022235-6ircruag 76 10 animal animal NN cord-022235-6ircruag 76 11 cells cell NNS cord-022235-6ircruag 76 12 . . . cord-022235-6ircruag 77 1 In in IN cord-022235-6ircruag 77 2 S. S. NNP cord-022235-6ircruag 77 3 cerevisiae cerevisiae NN cord-022235-6ircruag 77 4 cells cell NNS cord-022235-6ircruag 77 5 , , , cord-022235-6ircruag 77 6 a a DT cord-022235-6ircruag 77 7 single single JJ cord-022235-6ircruag 77 8 al-2-linked al-2-linked NNP cord-022235-6ircruag 77 9 mannose mannose NN cord-022235-6ircruag 77 10 residue residue NN cord-022235-6ircruag 77 11 is be VBZ cord-022235-6ircruag 77 12 replaced replace VBN cord-022235-6ircruag 77 13 by by IN cord-022235-6ircruag 77 14 αϊ-3 αϊ-3 NNP cord-022235-6ircruag 77 15 mannose mannose NN cord-022235-6ircruag 77 16 . . . cord-022235-6ircruag 78 1 A a DT cord-022235-6ircruag 78 2 mutation mutation NN cord-022235-6ircruag 78 3 in in IN cord-022235-6ircruag 78 4 the the DT cord-022235-6ircruag 78 5 GLS1 GLS1 NNP cord-022235-6ircruag 78 6 gene gene NN cord-022235-6ircruag 78 7 coding coding NN cord-022235-6ircruag 78 8 for for IN cord-022235-6ircruag 78 9 α α NN cord-022235-6ircruag 78 10 1 1 CD cord-022235-6ircruag 78 11 - - SYM cord-022235-6ircruag 78 12 2 2 CD cord-022235-6ircruag 78 13 glucosidase glucosidase NN cord-022235-6ircruag 78 14 does do VBZ cord-022235-6ircruag 78 15 not not RB cord-022235-6ircruag 78 16 affect affect VB cord-022235-6ircruag 78 17 removal removal NN cord-022235-6ircruag 78 18 of of IN cord-022235-6ircruag 78 19 the the DT cord-022235-6ircruag 78 20 mannose mannose NN cord-022235-6ircruag 78 21 residue residue NN cord-022235-6ircruag 78 22 or or CC cord-022235-6ircruag 78 23 subsequent subsequent JJ cord-022235-6ircruag 78 24 chain chain NN cord-022235-6ircruag 78 25 elongation elongation NN cord-022235-6ircruag 78 26 , , , cord-022235-6ircruag 78 27 whereas whereas IN cord-022235-6ircruag 78 28 removal removal NN cord-022235-6ircruag 78 29 of of IN cord-022235-6ircruag 78 30 the the DT cord-022235-6ircruag 78 31 ninth ninth JJ cord-022235-6ircruag 78 32 al-2-linked al-2-linked NNP cord-022235-6ircruag 78 33 mannose mannose NN cord-022235-6ircruag 78 34 residue residue NN cord-022235-6ircruag 78 35 may may MD cord-022235-6ircruag 78 36 be be VB cord-022235-6ircruag 78 37 essential essential JJ cord-022235-6ircruag 78 38 for for IN cord-022235-6ircruag 78 39 outer outer JJ cord-022235-6ircruag 78 40 chain chain NN cord-022235-6ircruag 78 41 elongation elongation NN cord-022235-6ircruag 78 42 , , , cord-022235-6ircruag 78 43 which which WDT cord-022235-6ircruag 78 44 occurs occur VBZ cord-022235-6ircruag 78 45 in in IN cord-022235-6ircruag 78 46 the the DT cord-022235-6ircruag 78 47 Golgi Golgi NNP cord-022235-6ircruag 78 48 ( ( -LRB- cord-022235-6ircruag 78 49 Section section NN cord-022235-6ircruag 78 50 V.D.I v.d.i NN cord-022235-6ircruag 78 51 ) ) -RRB- cord-022235-6ircruag 78 52 ( ( -LRB- cord-022235-6ircruag 78 53 598 598 CD cord-022235-6ircruag 78 54 ) ) -RRB- cord-022235-6ircruag 78 55 . . . cord-022235-6ircruag 79 1 Further further JJ cord-022235-6ircruag 79 2 processing processing NN cord-022235-6ircruag 79 3 of of IN cord-022235-6ircruag 79 4 animal animal NN cord-022235-6ircruag 79 5 cell cell NN cord-022235-6ircruag 79 6 glycoproteins glycoprotein NNS cord-022235-6ircruag 79 7 also also RB cord-022235-6ircruag 79 8 occurs occur VBZ cord-022235-6ircruag 79 9 in in IN cord-022235-6ircruag 79 10 the the DT cord-022235-6ircruag 79 11 Golgi Golgi NNP cord-022235-6ircruag 79 12 , , , cord-022235-6ircruag 79 13 although although IN cord-022235-6ircruag 79 14 further further JJ cord-022235-6ircruag 79 15 man man NN cord-022235-6ircruag 79 16 nose nose NN cord-022235-6ircruag 79 17 trimming trimming NN cord-022235-6ircruag 79 18 of of IN cord-022235-6ircruag 79 19 resident resident JJ cord-022235-6ircruag 79 20 ER ER NNP cord-022235-6ircruag 79 21 proteins protein NNS cord-022235-6ircruag 79 22 and and CC cord-022235-6ircruag 79 23 reglycosylation reglycosylation NN cord-022235-6ircruag 80 1 may may MD cord-022235-6ircruag 80 2 both both DT cord-022235-6ircruag 80 3 oc oc XX cord-022235-6ircruag 80 4 cur cur NN cord-022235-6ircruag 80 5 in in IN cord-022235-6ircruag 80 6 the the DT cord-022235-6ircruag 80 7 ER er NN cord-022235-6ircruag 80 8 ( ( -LRB- cord-022235-6ircruag 80 9 577,847 577,847 CD cord-022235-6ircruag 80 10 ) ) -RRB- cord-022235-6ircruag 80 11 . . . cord-022235-6ircruag 81 1 c. c. NNP cord-022235-6ircruag 81 2 Ser Ser NNP cord-022235-6ircruag 81 3 and and CC cord-022235-6ircruag 81 4 Thr Thr NNP cord-022235-6ircruag 81 5 residues residue NNS cord-022235-6ircruag 81 6 on on IN cord-022235-6ircruag 81 7 secretory secretory JJ cord-022235-6ircruag 81 8 proteins protein NNS cord-022235-6ircruag 81 9 of of IN cord-022235-6ircruag 81 10 yeasts yeast NNS cord-022235-6ircruag 81 11 and and CC cord-022235-6ircruag 81 12 possibly possibly RB cord-022235-6ircruag 81 13 other other JJ cord-022235-6ircruag 81 14 fungi fungus NNS cord-022235-6ircruag 81 15 can can MD cord-022235-6ircruag 81 16 be be VB cord-022235-6ircruag 81 17 O o NN cord-022235-6ircruag 81 18 - - HYPH cord-022235-6ircruag 81 19 glycosylated glycosylate VBN cord-022235-6ircruag 81 20 in in IN cord-022235-6ircruag 81 21 the the DT cord-022235-6ircruag 81 22 ER er NN cord-022235-6ircruag 81 23 ( ( -LRB- cord-022235-6ircruag 81 24 429 429 CD cord-022235-6ircruag 81 25 ) ) -RRB- cord-022235-6ircruag 81 26 . . . cord-022235-6ircruag 82 1 The the DT cord-022235-6ircruag 82 2 process process NN cord-022235-6ircruag 82 3 is be VBZ cord-022235-6ircruag 82 4 less less RBR cord-022235-6ircruag 82 5 well well RB cord-022235-6ircruag 82 6 characterized characterize VBN cord-022235-6ircruag 82 7 than than IN cord-022235-6ircruag 82 8 N n NN cord-022235-6ircruag 82 9 - - HYPH cord-022235-6ircruag 82 10 glycosylation glycosylation NN cord-022235-6ircruag 82 11 but but CC cord-022235-6ircruag 82 12 seems seem VBZ cord-022235-6ircruag 82 13 to to TO cord-022235-6ircruag 82 14 involve involve VB cord-022235-6ircruag 82 15 the the DT cord-022235-6ircruag 82 16 direct direct JJ cord-022235-6ircruag 82 17 trans trans JJ cord-022235-6ircruag 82 18 fer fer NN cord-022235-6ircruag 82 19 of of IN cord-022235-6ircruag 82 20 mannose mannose JJ cord-022235-6ircruag 82 21 residues residue NNS cord-022235-6ircruag 82 22 from from IN cord-022235-6ircruag 82 23 dolichol-1-mannose dolichol-1-mannose NNP cord-022235-6ircruag 82 24 onto onto IN cord-022235-6ircruag 82 25 acceptor acceptor NN cord-022235-6ircruag 82 26 amino amino NN cord-022235-6ircruag 82 27 acids acid NNS cord-022235-6ircruag 82 28 . . . cord-022235-6ircruag 83 1 Studies study NNS cord-022235-6ircruag 83 2 with with IN cord-022235-6ircruag 83 3 acceptor acceptor NN cord-022235-6ircruag 83 4 oligopeptides oligopeptide NNS cord-022235-6ircruag 83 5 suggest suggest VBP cord-022235-6ircruag 83 6 that that IN cord-022235-6ircruag 83 7 no no UH cord-022235-6ircruag 83 8 particular particular JJ cord-022235-6ircruag 83 9 se se FW cord-022235-6ircruag 83 10 quence quence NNP cord-022235-6ircruag 83 11 is be VBZ cord-022235-6ircruag 83 12 required require VBN cord-022235-6ircruag 83 13 around around IN cord-022235-6ircruag 83 14 the the DT cord-022235-6ircruag 83 15 modification modification NN cord-022235-6ircruag 83 16 site site NN cord-022235-6ircruag 83 17 ( ( -LRB- cord-022235-6ircruag 83 18 54,621 54,621 CD cord-022235-6ircruag 83 19 ) ) -RRB- cord-022235-6ircruag 83 20 . . . cord-022235-6ircruag 84 1 Further further JJ cord-022235-6ircruag 84 2 process process NN cord-022235-6ircruag 84 3 ing ing DT cord-022235-6ircruag 84 4 of of IN cord-022235-6ircruag 84 5 the the DT cord-022235-6ircruag 84 6 mannose mannose JJ cord-022235-6ircruag 84 7 residue residue NN cord-022235-6ircruag 84 8 occurs occur VBZ cord-022235-6ircruag 84 9 in in IN cord-022235-6ircruag 84 10 the the DT cord-022235-6ircruag 84 11 Golgi Golgi NNP cord-022235-6ircruag 84 12 apparatus apparatus NN cord-022235-6ircruag 84 13 ( ( -LRB- cord-022235-6ircruag 84 14 429 429 CD cord-022235-6ircruag 84 15 ) ) -RRB- cord-022235-6ircruag 85 1 ( ( -LRB- cord-022235-6ircruag 85 2 Section section NN cord-022235-6ircruag 85 3 V.D.2 V.D.2 NNP cord-022235-6ircruag 85 4 ) ) -RRB- cord-022235-6ircruag 85 5 . . . cord-022235-6ircruag 86 1 Yeast yeast NN cord-022235-6ircruag 86 2 secretory secretory NN cord-022235-6ircruag 86 3 proteins protein NNS cord-022235-6ircruag 86 4 may may MD cord-022235-6ircruag 86 5 be be VB cord-022235-6ircruag 86 6 both both CC cord-022235-6ircruag 86 7 N n CD cord-022235-6ircruag 86 8 - - : cord-022235-6ircruag 86 9 and and CC cord-022235-6ircruag 86 10 O o NN cord-022235-6ircruag 86 11 - - HYPH cord-022235-6ircruag 86 12 glycosylated glycosylated JJ cord-022235-6ircruag 86 13 . . . cord-022235-6ircruag 87 1 Most Most JJS cord-022235-6ircruag 87 2 studies study NNS cord-022235-6ircruag 87 3 indicate indicate VBP cord-022235-6ircruag 87 4 that that IN cord-022235-6ircruag 87 5 O o NN cord-022235-6ircruag 87 6 - - HYPH cord-022235-6ircruag 87 7 glycosylation glycosylation NN cord-022235-6ircruag 87 8 occurs occur VBZ cord-022235-6ircruag 87 9 exclusively exclusively RB cord-022235-6ircruag 87 10 in in IN cord-022235-6ircruag 87 11 the the DT cord-022235-6ircruag 87 12 Golgi Golgi NNP cord-022235-6ircruag 87 13 apparatus apparatus NN cord-022235-6ircruag 87 14 ( ( -LRB- cord-022235-6ircruag 87 15 Section section NN cord-022235-6ircruag 87 16 V.D.2 v.d.2 NN cord-022235-6ircruag 87 17 ) ) -RRB- cord-022235-6ircruag 87 18 . . . cord-022235-6ircruag 88 1 Secretory secretory JJ cord-022235-6ircruag 88 2 proteins protein NNS cord-022235-6ircruag 88 3 apparently apparently RB cord-022235-6ircruag 88 4 fold fold VBP cord-022235-6ircruag 88 5 spontaneously spontaneously RB cord-022235-6ircruag 88 6 as as IN cord-022235-6ircruag 88 7 they -PRON- PRP cord-022235-6ircruag 88 8 are be VBP cord-022235-6ircruag 88 9 extruded extrude VBN cord-022235-6ircruag 88 10 .across .across XX cord-022235-6ircruag 89 1 the the DT cord-022235-6ircruag 89 2 RER RER NNP cord-022235-6ircruag 89 3 membrane membrane NN cord-022235-6ircruag 89 4 ( ( -LRB- cord-022235-6ircruag 89 5 194 194 CD cord-022235-6ircruag 89 6 , , , cord-022235-6ircruag 89 7 1222 1222 CD cord-022235-6ircruag 89 8 ) ) -RRB- cord-022235-6ircruag 89 9 . . . cord-022235-6ircruag 90 1 However however RB cord-022235-6ircruag 90 2 , , , cord-022235-6ircruag 90 3 disulfide disulfide JJ cord-022235-6ircruag 90 4 bridge bridge NN cord-022235-6ircruag 90 5 forma forma NN cord-022235-6ircruag 90 6 tion tion NN cord-022235-6ircruag 90 7 is be VBZ cord-022235-6ircruag 90 8 probably probably RB cord-022235-6ircruag 90 9 catalyzed catalyze VBN cord-022235-6ircruag 90 10 by by IN cord-022235-6ircruag 90 11 protein protein NN cord-022235-6ircruag 90 12 disulfide disulfide NN cord-022235-6ircruag 90 13 isomerase isomerase NN cord-022235-6ircruag 90 14 ( ( -LRB- cord-022235-6ircruag 90 15 PDI PDI NNP cord-022235-6ircruag 90 16 ) ) -RRB- cord-022235-6ircruag 90 17 , , , cord-022235-6ircruag 90 18 which which WDT cord-022235-6ircruag 90 19 is be VBZ cord-022235-6ircruag 90 20 loosely loosely RB cord-022235-6ircruag 90 21 associated associate VBN cord-022235-6ircruag 90 22 with with IN cord-022235-6ircruag 90 23 the the DT cord-022235-6ircruag 90 24 RERM RERM NNP cord-022235-6ircruag 90 25 ( ( -LRB- cord-022235-6ircruag 90 26 325,326 325,326 CD cord-022235-6ircruag 90 27 ) ) -RRB- cord-022235-6ircruag 90 28 . . . cord-022235-6ircruag 91 1 The the DT cord-022235-6ircruag 91 2 formation formation NN cord-022235-6ircruag 91 3 of of IN cord-022235-6ircruag 91 4 soluble soluble JJ cord-022235-6ircruag 91 5 protein protein NN cord-022235-6ircruag 91 6 complexes complex NNS cord-022235-6ircruag 91 7 occurs occur VBZ cord-022235-6ircruag 91 8 shortly shortly RB cord-022235-6ircruag 91 9 after after IN cord-022235-6ircruag 91 10 synthesis synthesis NN cord-022235-6ircruag 91 11 ( ( -LRB- cord-022235-6ircruag 91 12 604 604 CD cord-022235-6ircruag 91 13 ) ) -RRB- cord-022235-6ircruag 91 14 , , , cord-022235-6ircruag 91 15 but but CC cord-022235-6ircruag 91 16 trimerization trimerization NN cord-022235-6ircruag 91 17 of of IN cord-022235-6ircruag 91 18 VSV VSV NNP cord-022235-6ircruag 91 19 G G NNP cord-022235-6ircruag 91 20 protein protein NN cord-022235-6ircruag 91 21 and and CC cord-022235-6ircruag 91 22 influenza influenza NN cord-022235-6ircruag 91 23 virus virus NN cord-022235-6ircruag 91 24 hemagglutinin hemagglutinin NN cord-022235-6ircruag 91 25 ( ( -LRB- cord-022235-6ircruag 91 26 HA HA NNP cord-022235-6ircruag 91 27 ) ) -RRB- cord-022235-6ircruag 91 28 , , , cord-022235-6ircruag 91 29 both both DT cord-022235-6ircruag 91 30 of of IN cord-022235-6ircruag 91 31 which which WDT cord-022235-6ircruag 91 32 are be VBP cord-022235-6ircruag 91 33 integral integral JJ cord-022235-6ircruag 91 34 membrane membrane NN cord-022235-6ircruag 91 35 proteins protein NNS cord-022235-6ircruag 91 36 , , , cord-022235-6ircruag 91 37 occurs occur VBZ cord-022235-6ircruag 91 38 only only RB cord-022235-6ircruag 91 39 7 7 CD cord-022235-6ircruag 91 40 - - SYM cord-022235-6ircruag 91 41 10 10 CD cord-022235-6ircruag 91 42 min min NN cord-022235-6ircruag 91 43 after after IN cord-022235-6ircruag 91 44 synthesis synthesis NN cord-022235-6ircruag 91 45 ( ( -LRB- cord-022235-6ircruag 91 46 193 193 CD cord-022235-6ircruag 91 47 , , , cord-022235-6ircruag 91 48 194 194 CD cord-022235-6ircruag 91 49 , , , cord-022235-6ircruag 91 50 364 364 CD cord-022235-6ircruag 91 51 , , , cord-022235-6ircruag 91 52 589 589 CD cord-022235-6ircruag 91 53 ) ) -RRB- cord-022235-6ircruag 91 54 and and CC cord-022235-6ircruag 91 55 involves involve VBZ cord-022235-6ircruag 91 56 the the DT cord-022235-6ircruag 91 57 selection selection NN cord-022235-6ircruag 91 58 of of IN cord-022235-6ircruag 91 59 polypeptides polypeptide NNS cord-022235-6ircruag 91 60 from from IN cord-022235-6ircruag 91 61 a a DT cord-022235-6ircruag 91 62 ran ran NN cord-022235-6ircruag 91 63 dom dom NN cord-022235-6ircruag 91 64 pool pool NN cord-022235-6ircruag 91 65 of of IN cord-022235-6ircruag 91 66 prefolded prefolded JJ cord-022235-6ircruag 91 67 monomers monomer NNS cord-022235-6ircruag 91 68 rather rather RB cord-022235-6ircruag 91 69 than than IN cord-022235-6ircruag 91 70 from from IN cord-022235-6ircruag 91 71 a a DT cord-022235-6ircruag 91 72 restricted restricted JJ cord-022235-6ircruag 91 73 pool pool NN cord-022235-6ircruag 91 74 of of IN cord-022235-6ircruag 91 75 monomers monomer NNS cord-022235-6ircruag 91 76 synthesized synthesize VBN cord-022235-6ircruag 91 77 on on IN cord-022235-6ircruag 91 78 a a DT cord-022235-6ircruag 91 79 single single JJ cord-022235-6ircruag 91 80 polysome polysome NN cord-022235-6ircruag 91 81 ( ( -LRB- cord-022235-6ircruag 91 82 108 108 CD cord-022235-6ircruag 91 83 ) ) -RRB- cord-022235-6ircruag 91 84 . . . cord-022235-6ircruag 92 1 Kreis Kreis NNP cord-022235-6ircruag 92 2 and and CC cord-022235-6ircruag 92 3 Lodish Lodish NNP cord-022235-6ircruag 92 4 ( ( -LRB- cord-022235-6ircruag 92 5 589 589 CD cord-022235-6ircruag 92 6 ) ) -RRB- cord-022235-6ircruag 92 7 suggest suggest VBP cord-022235-6ircruag 92 8 that that IN cord-022235-6ircruag 92 9 this this DT cord-022235-6ircruag 92 10 delay delay NN cord-022235-6ircruag 92 11 may may MD cord-022235-6ircruag 92 12 be be VB cord-022235-6ircruag 92 13 due due JJ cord-022235-6ircruag 92 14 to to IN cord-022235-6ircruag 92 15 the the DT cord-022235-6ircruag 92 16 segregation segregation NN cord-022235-6ircruag 92 17 of of IN cord-022235-6ircruag 92 18 PDI PDI NNP cord-022235-6ircruag 92 19 in in IN cord-022235-6ircruag 92 20 a a DT cord-022235-6ircruag 92 21 late late JJ cord-022235-6ircruag 92 22 " " `` cord-022235-6ircruag 92 23 compartment compartment NN cord-022235-6ircruag 92 24 " " '' cord-022235-6ircruag 92 25 of of IN cord-022235-6ircruag 92 26 the the DT cord-022235-6ircruag 92 27 ER ER NNP cord-022235-6ircruag 92 28 . . . cord-022235-6ircruag 93 1 According accord VBG cord-022235-6ircruag 93 2 to to IN cord-022235-6ircruag 93 3 the the DT cord-022235-6ircruag 93 4 papers paper NNS cord-022235-6ircruag 93 5 discussed discuss VBN cord-022235-6ircruag 93 6 above above RB cord-022235-6ircruag 93 7 , , , cord-022235-6ircruag 93 8 oligomerization oligomerization NN cord-022235-6ircruag 93 9 of of IN cord-022235-6ircruag 93 10 G G NNP cord-022235-6ircruag 93 11 and and CC cord-022235-6ircruag 93 12 HA HA NNP cord-022235-6ircruag 93 13 occurs occur VBZ cord-022235-6ircruag 93 14 just just RB cord-022235-6ircruag 93 15 before before IN cord-022235-6ircruag 93 16 they -PRON- PRP cord-022235-6ircruag 93 17 leave leave VBP cord-022235-6ircruag 93 18 the the DT cord-022235-6ircruag 93 19 ER ER NNP cord-022235-6ircruag 93 20 , , , cord-022235-6ircruag 93 21 5 5 CD cord-022235-6ircruag 93 22 - - SYM cord-022235-6ircruag 93 23 10 10 CD cord-022235-6ircruag 93 24 min min NN cord-022235-6ircruag 93 25 after after IN cord-022235-6ircruag 93 26 synthesis synthesis NN cord-022235-6ircruag 93 27 . . . cord-022235-6ircruag 94 1 This this DT cord-022235-6ircruag 94 2 view view NN cord-022235-6ircruag 94 3 is be VBZ cord-022235-6ircruag 94 4 challenged challenge VBN cord-022235-6ircruag 94 5 , , , cord-022235-6ircruag 94 6 however however RB cord-022235-6ircruag 94 7 , , , cord-022235-6ircruag 94 8 by by IN cord-022235-6ircruag 94 9 Yewdell Yewdell NNP cord-022235-6ircruag 94 10 et et NNP cord-022235-6ircruag 94 11 al al NNP cord-022235-6ircruag 94 12 . . . cord-022235-6ircruag 95 1 ( ( -LRB- cord-022235-6ircruag 95 2 1222 1222 CD cord-022235-6ircruag 95 3 ) ) -RRB- cord-022235-6ircruag 95 4 , , , cord-022235-6ircruag 95 5 who who WP cord-022235-6ircruag 95 6 found find VBD cord-022235-6ircruag 95 7 that that IN cord-022235-6ircruag 95 8 mono mono NN cord-022235-6ircruag 95 9 clonal clonal JJ cord-022235-6ircruag 95 10 antibodies antibody NNS cord-022235-6ircruag 95 11 specific specific JJ cord-022235-6ircruag 95 12 for for IN cord-022235-6ircruag 95 13 oligomerized oligomerize VBN cord-022235-6ircruag 95 14 HA HA NNP cord-022235-6ircruag 95 15 reacted react VBD cord-022235-6ircruag 95 16 only only RB cord-022235-6ircruag 95 17 with with IN cord-022235-6ircruag 95 18 proteins protein NNS cord-022235-6ircruag 95 19 in in IN cord-022235-6ircruag 95 20 the the DT cord-022235-6ircruag 95 21 Golgi Golgi NNP cord-022235-6ircruag 95 22 apparatus apparatus NN cord-022235-6ircruag 95 23 , , , cord-022235-6ircruag 95 24 and and CC cord-022235-6ircruag 95 25 not not RB cord-022235-6ircruag 95 26 with with IN cord-022235-6ircruag 95 27 HA HA NNP cord-022235-6ircruag 95 28 in in IN cord-022235-6ircruag 95 29 the the DT cord-022235-6ircruag 95 30 ER er NN cord-022235-6ircruag 95 31 ( ( -LRB- cord-022235-6ircruag 95 32 see see VB cord-022235-6ircruag 95 33 next next JJ cord-022235-6ircruag 95 34 section section NN cord-022235-6ircruag 95 35 ) ) -RRB- cord-022235-6ircruag 95 36 . . . cord-022235-6ircruag 96 1 A a DT cord-022235-6ircruag 96 2 possible possible JJ cord-022235-6ircruag 96 3 explanation explanation NN cord-022235-6ircruag 96 4 for for IN cord-022235-6ircruag 96 5 this this DT cord-022235-6ircruag 96 6 ambiguity ambiguity NN cord-022235-6ircruag 96 7 is be VBZ cord-022235-6ircruag 96 8 that that IN cord-022235-6ircruag 96 9 HA HA NNP cord-022235-6ircruag 96 10 monomers monomer NNS cord-022235-6ircruag 96 11 fold fold VBP cord-022235-6ircruag 96 12 and and CC cord-022235-6ircruag 96 13 trimerize trimerize ADD cord-022235-6ircruag 96 14 in in IN cord-022235-6ircruag 96 15 the the DT cord-022235-6ircruag 96 16 ER ER NNP cord-022235-6ircruag 96 17 and and CC cord-022235-6ircruag 96 18 are be VBP cord-022235-6ircruag 96 19 then then RB cord-022235-6ircruag 96 20 transported transport VBN cord-022235-6ircruag 96 21 to to IN cord-022235-6ircruag 96 22 the the DT cord-022235-6ircruag 96 23 Golgi Golgi NNP cord-022235-6ircruag 96 24 apparatus apparatus NN cord-022235-6ircruag 96 25 , , , cord-022235-6ircruag 96 26 where where WRB cord-022235-6ircruag 96 27 further further JJ cord-022235-6ircruag 96 28 modifications modification NNS cord-022235-6ircruag 96 29 alter alter VBP cord-022235-6ircruag 96 30 the the DT cord-022235-6ircruag 96 31 conformation conformation NN cord-022235-6ircruag 96 32 of of IN cord-022235-6ircruag 96 33 the the DT cord-022235-6ircruag 96 34 HA HA NNP cord-022235-6ircruag 96 35 trimers trimer NNS cord-022235-6ircruag 96 36 to to TO cord-022235-6ircruag 96 37 produce produce VB cord-022235-6ircruag 96 38 the the DT cord-022235-6ircruag 96 39 antigenic antigenic JJ cord-022235-6ircruag 96 40 sites site NNS cord-022235-6ircruag 96 41 recognized recognize VBN cord-022235-6ircruag 96 42 by by IN cord-022235-6ircruag 96 43 the the DT cord-022235-6ircruag 96 44 antibody antibody NN cord-022235-6ircruag 96 45 used use VBN cord-022235-6ircruag 96 46 by by IN cord-022235-6ircruag 96 47 Yewdell Yewdell NNP cord-022235-6ircruag 96 48 et et NNP cord-022235-6ircruag 96 49 al al NNP cord-022235-6ircruag 96 50 . . . cord-022235-6ircruag 97 1 Different different JJ cord-022235-6ircruag 97 2 proteins protein NNS cord-022235-6ircruag 97 3 transit transit NN cord-022235-6ircruag 97 4 through through IN cord-022235-6ircruag 97 5 the the DT cord-022235-6ircruag 97 6 secretory secretory JJ cord-022235-6ircruag 97 7 pathway pathway NN cord-022235-6ircruag 97 8 together together RB cord-022235-6ircruag 97 9 , , , cord-022235-6ircruag 97 10 but but CC cord-022235-6ircruag 97 11 the the DT cord-022235-6ircruag 97 12 rates rate NNS cord-022235-6ircruag 97 13 at at IN cord-022235-6ircruag 97 14 which which WDT cord-022235-6ircruag 97 15 they -PRON- PRP cord-022235-6ircruag 97 16 are be VBP cord-022235-6ircruag 97 17 secreted secrete VBN cord-022235-6ircruag 97 18 may may MD cord-022235-6ircruag 97 19 vary vary VB cord-022235-6ircruag 97 20 considerably considerably RB cord-022235-6ircruag 97 21 ( ( -LRB- cord-022235-6ircruag 97 22 335 335 CD cord-022235-6ircruag 97 23 , , , cord-022235-6ircruag 97 24 343 343 CD cord-022235-6ircruag 97 25 , , , cord-022235-6ircruag 97 26 615 615 CD cord-022235-6ircruag 97 27 , , , cord-022235-6ircruag 97 28 1221 1221 CD cord-022235-6ircruag 97 29 ) ) -RRB- cord-022235-6ircruag 97 30 . . . cord-022235-6ircruag 98 1 The the DT cord-022235-6ircruag 98 2 site site NN cord-022235-6ircruag 98 3 at at IN cord-022235-6ircruag 98 4 which which WDT cord-022235-6ircruag 98 5 the the DT cord-022235-6ircruag 98 6 secretion secretion NN cord-022235-6ircruag 98 7 lag lag NN cord-022235-6ircruag 98 8 is be VBZ cord-022235-6ircruag 98 9 most most RBS cord-022235-6ircruag 98 10 prominent prominent JJ cord-022235-6ircruag 98 11 seems seem NNS cord-022235-6ircruag 98 12 to to TO cord-022235-6ircruag 98 13 be be VB cord-022235-6ircruag 98 14 transit transit NN cord-022235-6ircruag 98 15 from from IN cord-022235-6ircruag 98 16 the the DT cord-022235-6ircruag 98 17 RER RER NNP cord-022235-6ircruag 98 18 to to IN cord-022235-6ircruag 98 19 the the DT cord-022235-6ircruag 98 20 Golgi Golgi NNP cord-022235-6ircruag 98 21 ( ( -LRB- cord-022235-6ircruag 98 22 646 646 CD cord-022235-6ircruag 98 23 ) ) -RRB- cord-022235-6ircruag 98 24 . . . cord-022235-6ircruag 99 1 It -PRON- PRP cord-022235-6ircruag 99 2 has have VBZ cord-022235-6ircruag 99 3 been be VBN cord-022235-6ircruag 99 4 suggested suggest VBN cord-022235-6ircruag 99 5 that that IN cord-022235-6ircruag 99 6 this this DT cord-022235-6ircruag 99 7 delay delay NN cord-022235-6ircruag 99 8 might may MD cord-022235-6ircruag 99 9 reflect reflect VB cord-022235-6ircruag 99 10 the the DT cord-022235-6ircruag 99 11 need need NN cord-022235-6ircruag 99 12 for for IN cord-022235-6ircruag 99 13 secretory secretory JJ cord-022235-6ircruag 99 14 proteins protein NNS cord-022235-6ircruag 99 15 to to TO cord-022235-6ircruag 99 16 interact interact VB cord-022235-6ircruag 99 17 with with IN cord-022235-6ircruag 99 18 specific specific JJ cord-022235-6ircruag 99 19 receptors receptor NNS cord-022235-6ircruag 99 20 in in IN cord-022235-6ircruag 99 21 the the DT cord-022235-6ircruag 99 22 RER rer NN cord-022235-6ircruag 99 23 for for IN cord-022235-6ircruag 99 24 transport transport NN cord-022235-6ircruag 99 25 to to IN cord-022235-6ircruag 99 26 the the DT cord-022235-6ircruag 99 27 Golgi Golgi NNP cord-022235-6ircruag 99 28 and and CC cord-022235-6ircruag 99 29 that that IN cord-022235-6ircruag 99 30 carbohydrates carbohydrate NNS cord-022235-6ircruag 99 31 might may MD cord-022235-6ircruag 99 32 form form VB cord-022235-6ircruag 99 33 part part NN cord-022235-6ircruag 99 34 of of IN cord-022235-6ircruag 99 35 the the DT cord-022235-6ircruag 99 36 recognition recognition NN cord-022235-6ircruag 99 37 signal signal NN cord-022235-6ircruag 99 38 ( ( -LRB- cord-022235-6ircruag 99 39 320,647,648 320,647,648 NN cord-022235-6ircruag 99 40 ) ) -RRB- cord-022235-6ircruag 99 41 . . . cord-022235-6ircruag 100 1 Alternatively alternatively RB cord-022235-6ircruag 100 2 , , , cord-022235-6ircruag 100 3 proteins protein NNS cord-022235-6ircruag 100 4 may may MD cord-022235-6ircruag 100 5 be be VB cord-022235-6ircruag 100 6 retained retain VBN cord-022235-6ircruag 100 7 in in IN cord-022235-6ircruag 100 8 the the DT cord-022235-6ircruag 100 9 ER ER NNP cord-022235-6ircruag 100 10 until until IN cord-022235-6ircruag 100 11 they -PRON- PRP cord-022235-6ircruag 100 12 have have VBP cord-022235-6ircruag 100 13 folded fold VBN cord-022235-6ircruag 100 14 correctly correctly RB cord-022235-6ircruag 100 15 ; ; : cord-022235-6ircruag 100 16 different different JJ cord-022235-6ircruag 100 17 folding folding NN cord-022235-6ircruag 100 18 kinetics kinetic NNS cord-022235-6ircruag 100 19 may may MD cord-022235-6ircruag 100 20 result result VB cord-022235-6ircruag 100 21 in in IN cord-022235-6ircruag 100 22 different different JJ cord-022235-6ircruag 100 23 retention retention NN cord-022235-6ircruag 100 24 times time NNS cord-022235-6ircruag 100 25 in in IN cord-022235-6ircruag 100 26 the the DT cord-022235-6ircruag 100 27 ER ER NNP cord-022235-6ircruag 100 28 ( ( -LRB- cord-022235-6ircruag 100 29 589,1192 589,1192 CD cord-022235-6ircruag 100 30 ) ) -RRB- cord-022235-6ircruag 100 31 . . . cord-022235-6ircruag 101 1 Indeed indeed RB cord-022235-6ircruag 101 2 , , , cord-022235-6ircruag 101 3 exit exit NN cord-022235-6ircruag 101 4 from from IN cord-022235-6ircruag 101 5 the the DT cord-022235-6ircruag 101 6 ER ER NNP cord-022235-6ircruag 101 7 of of IN cord-022235-6ircruag 101 8 one one CD cord-022235-6ircruag 101 9 of of IN cord-022235-6ircruag 101 10 the the DT cord-022235-6ircruag 101 11 proteins protein NNS cord-022235-6ircruag 101 12 studied study VBN cord-022235-6ircruag 101 13 by by IN cord-022235-6ircruag 101 14 Fitting Fitting NNP cord-022235-6ircruag 101 15 and and CC cord-022235-6ircruag 101 16 Kabat Kabat NNP cord-022235-6ircruag 101 17 ( ( -LRB- cord-022235-6ircruag 101 18 320 320 CD cord-022235-6ircruag 101 19 ) ) -RRB- cord-022235-6ircruag 101 20 coincided coincide VBD cord-022235-6ircruag 101 21 with with IN cord-022235-6ircruag 101 22 a a DT cord-022235-6ircruag 101 23 partial partial JJ cord-022235-6ircruag 101 24 proteolysis proteolysis NN cord-022235-6ircruag 101 25 event event NN cord-022235-6ircruag 101 26 , , , cord-022235-6ircruag 101 27 and and CC cord-022235-6ircruag 101 28 polypeptides polypeptide NNS cord-022235-6ircruag 101 29 were be VBD cord-022235-6ircruag 101 30 " " `` cord-022235-6ircruag 101 31 selected select VBN cord-022235-6ircruag 101 32 " " '' cord-022235-6ircruag 101 33 for for IN cord-022235-6ircruag 101 34 exit exit NN cord-022235-6ircruag 101 35 from from IN cord-022235-6ircruag 101 36 a a DT cord-022235-6ircruag 101 37 random random JJ cord-022235-6ircruag 101 38 pool pool NN cord-022235-6ircruag 101 39 of of IN cord-022235-6ircruag 101 40 " " `` cord-022235-6ircruag 101 41 new new JJ cord-022235-6ircruag 101 42 " " '' cord-022235-6ircruag 101 43 and and CC cord-022235-6ircruag 101 44 " " `` cord-022235-6ircruag 101 45 old old JJ cord-022235-6ircruag 101 46 " " '' cord-022235-6ircruag 101 47 proteins protein NNS cord-022235-6ircruag 101 48 . . . cord-022235-6ircruag 102 1 Furthermore furthermore RB cord-022235-6ircruag 102 2 , , , cord-022235-6ircruag 102 3 studies study NNS cord-022235-6ircruag 102 4 discussed discuss VBN cord-022235-6ircruag 102 5 in in IN cord-022235-6ircruag 102 6 the the DT cord-022235-6ircruag 102 7 preced prece VBN cord-022235-6ircruag 102 8 ing ing NNP cord-022235-6ircruag 102 9 section section NN cord-022235-6ircruag 102 10 showed show VBD cord-022235-6ircruag 102 11 that that IN cord-022235-6ircruag 102 12 oligomerization oligomerization NN cord-022235-6ircruag 102 13 of of IN cord-022235-6ircruag 102 14 virus virus NN cord-022235-6ircruag 102 15 - - HYPH cord-022235-6ircruag 102 16 encoded encode VBN cord-022235-6ircruag 102 17 membrane membrane NN cord-022235-6ircruag 102 18 pro pro JJ cord-022235-6ircruag 102 19 teins tein NNS cord-022235-6ircruag 102 20 occurs occur VBZ cord-022235-6ircruag 102 21 just just RB cord-022235-6ircruag 102 22 before before IN cord-022235-6ircruag 102 23 they -PRON- PRP cord-022235-6ircruag 102 24 are be VBP cord-022235-6ircruag 102 25 transported transport VBN cord-022235-6ircruag 102 26 to to IN cord-022235-6ircruag 102 27 the the DT cord-022235-6ircruag 102 28 Golgi Golgi NNP cord-022235-6ircruag 102 29 . . . cord-022235-6ircruag 103 1 If if IN cord-022235-6ircruag 103 2 secretory secretory JJ cord-022235-6ircruag 103 3 proteins protein NNS cord-022235-6ircruag 103 4 are be VBP cord-022235-6ircruag 103 5 indeed indeed RB cord-022235-6ircruag 103 6 retained retain VBN cord-022235-6ircruag 103 7 in in IN cord-022235-6ircruag 103 8 the the DT cord-022235-6ircruag 103 9 ER ER NNP cord-022235-6ircruag 103 10 until until IN cord-022235-6ircruag 103 11 they -PRON- PRP cord-022235-6ircruag 103 12 are be VBP cord-022235-6ircruag 103 13 folded fold VBN cord-022235-6ircruag 103 14 and and CC cord-022235-6ircruag 103 15 oligomerized oligomerize VBN cord-022235-6ircruag 103 16 in in IN cord-022235-6ircruag 103 17 the the DT cord-022235-6ircruag 103 18 correct correct JJ cord-022235-6ircruag 103 19 way way NN cord-022235-6ircruag 103 20 , , , cord-022235-6ircruag 103 21 then then RB cord-022235-6ircruag 103 22 exit exit NN cord-022235-6ircruag 103 23 - - HYPH cord-022235-6ircruag 103 24 incompetent incompetent JJ cord-022235-6ircruag 103 25 proteins protein NNS cord-022235-6ircruag 103 26 should should MD cord-022235-6ircruag 103 27 either either CC cord-022235-6ircruag 103 28 remain remain VB cord-022235-6ircruag 103 29 indefinitely indefinitely RB cord-022235-6ircruag 103 30 in in IN cord-022235-6ircruag 103 31 the the DT cord-022235-6ircruag 103 32 ER er NN cord-022235-6ircruag 103 33 or or CC cord-022235-6ircruag 103 34 be be VB cord-022235-6ircruag 103 35 secreted secrete VBN cord-022235-6ircruag 103 36 at at IN cord-022235-6ircruag 103 37 very very RB cord-022235-6ircruag 103 38 much much RB cord-022235-6ircruag 103 39 reduced reduce VBN cord-022235-6ircruag 103 40 rates rate NNS cord-022235-6ircruag 103 41 . . . cord-022235-6ircruag 104 1 This this DT cord-022235-6ircruag 104 2 could could MD cord-022235-6ircruag 104 3 explain explain VB cord-022235-6ircruag 104 4 why why WRB cord-022235-6ircruag 104 5 genetically genetically RB cord-022235-6ircruag 104 6 altered altered JJ cord-022235-6ircruag 104 7 or or CC cord-022235-6ircruag 104 8 hybrid hybrid JJ cord-022235-6ircruag 104 9 pro pro FW cord-022235-6ircruag 104 10 teins tein NNS cord-022235-6ircruag 104 11 are be VBP cord-022235-6ircruag 104 12 sometimes sometimes RB cord-022235-6ircruag 104 13 translocated translocate VBN cord-022235-6ircruag 104 14 into into IN cord-022235-6ircruag 104 15 the the DT cord-022235-6ircruag 104 16 RER rer NN cord-022235-6ircruag 104 17 without without IN cord-022235-6ircruag 104 18 difficulty difficulty NN cord-022235-6ircruag 104 19 and and CC cord-022235-6ircruag 104 20 yet yet RB cord-022235-6ircruag 104 21 do do VBP cord-022235-6ircruag 104 22 not not RB cord-022235-6ircruag 104 23 transit transit VB cord-022235-6ircruag 104 24 further far RBR cord-022235-6ircruag 104 25 through through IN cord-022235-6ircruag 104 26 the the DT cord-022235-6ircruag 104 27 secretory secretory JJ cord-022235-6ircruag 104 28 pathway pathway NN cord-022235-6ircruag 104 29 ( ( -LRB- cord-022235-6ircruag 104 30 104 104 CD cord-022235-6ircruag 104 31 , , , cord-022235-6ircruag 104 32 281 281 CD cord-022235-6ircruag 104 33 , , , cord-022235-6ircruag 104 34 364 364 CD cord-022235-6ircruag 104 35 ) ) -RRB- cord-022235-6ircruag 104 36 , , , cord-022235-6ircruag 104 37 and and CC cord-022235-6ircruag 104 38 why why WRB cord-022235-6ircruag 104 39 incomplete incomplete JJ cord-022235-6ircruag 104 40 glycosylation glycosylation NN cord-022235-6ircruag 104 41 or or CC cord-022235-6ircruag 104 42 glucose glucose NN cord-022235-6ircruag 104 43 trimming trimming NN cord-022235-6ircruag 104 44 and and CC cord-022235-6ircruag 104 45 failure failure NN cord-022235-6ircruag 104 46 to to TO cord-022235-6ircruag 104 47 process process NN cord-022235-6ircruag 104 48 signal signal NN cord-022235-6ircruag 104 49 peptides peptide NNS cord-022235-6ircruag 104 50 sometimes sometimes RB cord-022235-6ircruag 104 51 affect affect VBP cord-022235-6ircruag 104 52 secretion secretion NN cord-022235-6ircruag 104 53 kinetics kinetic NNS cord-022235-6ircruag 104 54 ( ( -LRB- cord-022235-6ircruag 104 55 96 96 CD cord-022235-6ircruag 104 56 , , , cord-022235-6ircruag 104 57 577 577 CD cord-022235-6ircruag 104 58 , , , cord-022235-6ircruag 104 59 649 649 CD cord-022235-6ircruag 104 60 , , , cord-022235-6ircruag 104 61 1045 1045 CD cord-022235-6ircruag 104 62 ) ) -RRB- cord-022235-6ircruag 104 63 . . . cord-022235-6ircruag 105 1 Even even RB cord-022235-6ircruag 105 2 minor minor JJ cord-022235-6ircruag 105 3 sequence sequence NN cord-022235-6ircruag 105 4 changes change NNS cord-022235-6ircruag 105 5 have have VBP cord-022235-6ircruag 105 6 been be VBN cord-022235-6ircruag 105 7 reported report VBN cord-022235-6ircruag 105 8 to to TO cord-022235-6ircruag 105 9 affect affect VB cord-022235-6ircruag 105 10 protein protein NN cord-022235-6ircruag 105 11 con con NN cord-022235-6ircruag 105 12 formation formation NN cord-022235-6ircruag 105 13 and and CC cord-022235-6ircruag 105 14 exit exit NN cord-022235-6ircruag 105 15 from from IN cord-022235-6ircruag 105 16 the the DT cord-022235-6ircruag 105 17 ER ER NNP cord-022235-6ircruag 105 18 ( ( -LRB- cord-022235-6ircruag 105 19 1192 1192 CD cord-022235-6ircruag 105 20 ) ) -RRB- cord-022235-6ircruag 105 21 . . . cord-022235-6ircruag 106 1 What what WP cord-022235-6ircruag 106 2 happens happen VBZ cord-022235-6ircruag 106 3 to to TO cord-022235-6ircruag 106 4 incorrectly incorrectly RB cord-022235-6ircruag 106 5 folded fold VBN cord-022235-6ircruag 106 6 proteins protein NNS cord-022235-6ircruag 106 7 in in IN cord-022235-6ircruag 106 8 the the DT cord-022235-6ircruag 106 9 ER er NN cord-022235-6ircruag 106 10 ? ? . cord-022235-6ircruag 107 1 Results result NNS cord-022235-6ircruag 107 2 from from IN cord-022235-6ircruag 107 3 numerous numerous JJ cord-022235-6ircruag 107 4 studies study NNS cord-022235-6ircruag 107 5 indicate indicate VBP cord-022235-6ircruag 107 6 that that IN cord-022235-6ircruag 107 7 they -PRON- PRP cord-022235-6ircruag 107 8 stay stay VBP cord-022235-6ircruag 107 9 in in IN cord-022235-6ircruag 107 10 the the DT cord-022235-6ircruag 107 11 ER er NN cord-022235-6ircruag 107 12 ( ( -LRB- cord-022235-6ircruag 107 13 604,681 604,681 CD cord-022235-6ircruag 107 14 ) ) -RRB- cord-022235-6ircruag 107 15 or or CC cord-022235-6ircruag 107 16 are be VBP cord-022235-6ircruag 107 17 de de FW cord-022235-6ircruag 107 18 graded grade VBN cord-022235-6ircruag 107 19 either either CC cord-022235-6ircruag 107 20 in in IN cord-022235-6ircruag 107 21 the the DT cord-022235-6ircruag 107 22 ER er NN cord-022235-6ircruag 107 23 ( ( -LRB- cord-022235-6ircruag 107 24 642a 642a NNP cord-022235-6ircruag 107 25 ) ) -RRB- cord-022235-6ircruag 107 26 or or CC cord-022235-6ircruag 107 27 in in IN cord-022235-6ircruag 107 28 the the DT cord-022235-6ircruag 107 29 lysosome lysosome NN cord-022235-6ircruag 107 30 ( ( -LRB- cord-022235-6ircruag 107 31 723 723 CD cord-022235-6ircruag 107 32 ) ) -RRB- cord-022235-6ircruag 107 33 . . . cord-022235-6ircruag 108 1 These these DT cord-022235-6ircruag 108 2 proteins protein NNS cord-022235-6ircruag 108 3 do do VBP cord-022235-6ircruag 108 4 not not RB cord-022235-6ircruag 108 5 seem seem VB cord-022235-6ircruag 108 6 to to TO cord-022235-6ircruag 108 7 precipitate precipitate VB cord-022235-6ircruag 108 8 in in IN cord-022235-6ircruag 108 9 large large JJ cord-022235-6ircruag 108 10 aggregates aggregate NNS cord-022235-6ircruag 108 11 . . . cord-022235-6ircruag 109 1 Instead instead RB cord-022235-6ircruag 109 2 , , , cord-022235-6ircruag 109 3 a a DT cord-022235-6ircruag 109 4 specific specific JJ cord-022235-6ircruag 109 5 , , , cord-022235-6ircruag 109 6 major major JJ cord-022235-6ircruag 109 7 ER ER NNP cord-022235-6ircruag 109 8 protein protein NN cord-022235-6ircruag 109 9 seems seem VBZ cord-022235-6ircruag 109 10 to to TO cord-022235-6ircruag 109 11 bind bind VB cord-022235-6ircruag 109 12 to to IN cord-022235-6ircruag 109 13 some some DT cord-022235-6ircruag 109 14 incorrectly incorrectly RB cord-022235-6ircruag 109 15 folded fold VBN cord-022235-6ircruag 109 16 proteins protein NNS cord-022235-6ircruag 109 17 , , , cord-022235-6ircruag 109 18 thereby thereby RB cord-022235-6ircruag 109 19 pre pre VB cord-022235-6ircruag 109 20 venting vent VBG cord-022235-6ircruag 109 21 their -PRON- PRP$ cord-022235-6ircruag 109 22 exit exit NN cord-022235-6ircruag 109 23 from from IN cord-022235-6ircruag 109 24 the the DT cord-022235-6ircruag 109 25 ER ER NNP cord-022235-6ircruag 109 26 . . . cord-022235-6ircruag 110 1 Haas Haas NNP cord-022235-6ircruag 110 2 and and CC cord-022235-6ircruag 110 3 Wabl Wabl NNP cord-022235-6ircruag 110 4 ( ( -LRB- cord-022235-6ircruag 110 5 413 413 CD cord-022235-6ircruag 110 6 ) ) -RRB- cord-022235-6ircruag 110 7 first first RB cord-022235-6ircruag 110 8 detected detect VBD cord-022235-6ircruag 110 9 this this DT cord-022235-6ircruag 110 10 protein protein NN cord-022235-6ircruag 110 11 ( ( -LRB- cord-022235-6ircruag 110 12 BiP BiP NNP cord-022235-6ircruag 110 13 ) ) -RRB- cord-022235-6ircruag 111 1 complexed complexe VBN cord-022235-6ircruag 111 2 with with IN cord-022235-6ircruag 111 3 immunoglobulin immunoglobulin NN cord-022235-6ircruag 111 4 heavy heavy JJ cord-022235-6ircruag 111 5 chains chain NNS cord-022235-6ircruag 111 6 synthesized synthesize VBN cord-022235-6ircruag 111 7 in in IN cord-022235-6ircruag 111 8 the the DT cord-022235-6ircruag 111 9 absence absence NN cord-022235-6ircruag 111 10 of of IN cord-022235-6ircruag 111 11 light light JJ cord-022235-6ircruag 111 12 chains chain NNS cord-022235-6ircruag 111 13 ( ( -LRB- cord-022235-6ircruag 111 14 heavy heavy JJ cord-022235-6ircruag 111 15 and and CC cord-022235-6ircruag 111 16 light light JJ cord-022235-6ircruag 111 17 chains chain NNS cord-022235-6ircruag 111 18 are be VBP cord-022235-6ircruag 111 19 only only RB cord-022235-6ircruag 111 20 transported transport VBN cord-022235-6ircruag 111 21 to to IN cord-022235-6ircruag 111 22 the the DT cord-022235-6ircruag 111 23 Golgi Golgi NNP cord-022235-6ircruag 111 24 as as IN cord-022235-6ircruag 111 25 complexes complex NNS cord-022235-6ircruag 111 26 ) ) -RRB- cord-022235-6ircruag 111 27 ( ( -LRB- cord-022235-6ircruag 111 28 97 97 CD cord-022235-6ircruag 111 29 ) ) -RRB- cord-022235-6ircruag 111 30 , , , cord-022235-6ircruag 111 31 and and CC cord-022235-6ircruag 111 32 it -PRON- PRP cord-022235-6ircruag 111 33 was be VBD cord-022235-6ircruag 111 34 subsequently subsequently RB cord-022235-6ircruag 111 35 found find VBN cord-022235-6ircruag 111 36 to to TO cord-022235-6ircruag 111 37 be be VB cord-022235-6ircruag 111 38 complexed complexe VBN cord-022235-6ircruag 111 39 with with IN cord-022235-6ircruag 111 40 incorrectly incorrectly RB cord-022235-6ircruag 111 41 oligomerized oligomerize VBN cord-022235-6ircruag 111 42 or or CC cord-022235-6ircruag 111 43 monomeric monomeric JJ cord-022235-6ircruag 111 44 HA HA NNP cord-022235-6ircruag 111 45 ( ( -LRB- cord-022235-6ircruag 111 46 194 194 CD cord-022235-6ircruag 111 47 , , , cord-022235-6ircruag 111 48 364 364 CD cord-022235-6ircruag 111 49 ) ) -RRB- cord-022235-6ircruag 111 50 , , , cord-022235-6ircruag 111 51 nonglycosylated nonglycosylated NNP cord-022235-6ircruag 111 52 invertase invertase NNP cord-022235-6ircruag 111 53 ( ( -LRB- cord-022235-6ircruag 111 54 in in IN cord-022235-6ircruag 111 55 vitro vitro FW cord-022235-6ircruag 111 56 in in IN cord-022235-6ircruag 111 57 dog dog NN cord-022235-6ircruag 111 58 pancreatic pancreatic NN cord-022235-6ircruag 111 59 microsomes microsome NNS cord-022235-6ircruag 111 60 ) ) -RRB- cord-022235-6ircruag 111 61 and and CC cord-022235-6ircruag 111 62 incorrectly incorrectly RB cord-022235-6ircruag 111 63 folded fold VBD cord-022235-6ircruag 111 64 prolactin prolactin NN cord-022235-6ircruag 111 65 ( ( -LRB- cord-022235-6ircruag 111 66 557 557 CD cord-022235-6ircruag 111 67 ) ) -RRB- cord-022235-6ircruag 111 68 , , , cord-022235-6ircruag 111 69 and and CC cord-022235-6ircruag 111 70 recombinant recombinant JJ cord-022235-6ircruag 111 71 human human JJ cord-022235-6ircruag 111 72 factor factor NN cord-022235-6ircruag 111 73 VII VII NNP cord-022235-6ircruag 111 74 in in IN cord-022235-6ircruag 111 75 Chinese chinese JJ cord-022235-6ircruag 111 76 hamster hamster NN cord-022235-6ircruag 111 77 ovary ovary NN cord-022235-6ircruag 111 78 cells cell NNS cord-022235-6ircruag 111 79 ( ( -LRB- cord-022235-6ircruag 111 80 561 561 CD cord-022235-6ircruag 111 81 ) ) -RRB- cord-022235-6ircruag 111 82 . . . cord-022235-6ircruag 112 1 It -PRON- PRP cord-022235-6ircruag 112 2 was be VBD cord-022235-6ircruag 112 3 not not RB cord-022235-6ircruag 112 4 detected detect VBN cord-022235-6ircruag 112 5 in in IN cord-022235-6ircruag 112 6 association association NN cord-022235-6ircruag 112 7 with with IN cord-022235-6ircruag 112 8 aggregated aggregate VBN cord-022235-6ircruag 112 9 VSV VSV NNP cord-022235-6ircruag 112 10 G G NNP cord-022235-6ircruag 112 11 protein protein NN cord-022235-6ircruag 112 12 ( ( -LRB- cord-022235-6ircruag 112 13 250 250 CD cord-022235-6ircruag 112 14 ) ) -RRB- cord-022235-6ircruag 112 15 or or CC cord-022235-6ircruag 112 16 with with IN cord-022235-6ircruag 112 17 incorrectly incorrectly RB cord-022235-6ircruag 112 18 folded fold VBN cord-022235-6ircruag 112 19 ( ( -LRB- cord-022235-6ircruag 112 20 mutant mutant JJ cord-022235-6ircruag 112 21 ) ) -RRB- cord-022235-6ircruag 112 22 class class NN cord-022235-6ircruag 112 23 I i NN cord-022235-6ircruag 112 24 histocompatibility histocompatibility NN cord-022235-6ircruag 112 25 antigen antigen NN cord-022235-6ircruag 112 26 ( ( -LRB- cord-022235-6ircruag 112 27 1192 1192 CD cord-022235-6ircruag 112 28 ) ) -RRB- cord-022235-6ircruag 112 29 . . . cord-022235-6ircruag 113 1 The the DT cord-022235-6ircruag 113 2 dissociation dissociation NN cord-022235-6ircruag 113 3 of of IN cord-022235-6ircruag 113 4 secretory secretory JJ cord-022235-6ircruag 113 5 protein protein NN cord-022235-6ircruag 113 6 - - HYPH cord-022235-6ircruag 113 7 BiP BiP NNP cord-022235-6ircruag 113 8 complexes complex NNS cord-022235-6ircruag 113 9 requires require VBZ cord-022235-6ircruag 113 10 ATP ATP NNP cord-022235-6ircruag 113 11 ( ( -LRB- cord-022235-6ircruag 113 12 758 758 CD cord-022235-6ircruag 113 13 ) ) -RRB- cord-022235-6ircruag 113 14 , , , cord-022235-6ircruag 113 15 which which WDT cord-022235-6ircruag 113 16 might may MD cord-022235-6ircruag 113 17 explain explain VB cord-022235-6ircruag 113 18 part part NN cord-022235-6ircruag 113 19 of of IN cord-022235-6ircruag 113 20 the the DT cord-022235-6ircruag 113 21 ATP ATP NNP cord-022235-6ircruag 113 22 requirement requirement NN cord-022235-6ircruag 113 23 for for IN cord-022235-6ircruag 113 24 protein protein NN cord-022235-6ircruag 113 25 movement movement NN cord-022235-6ircruag 113 26 along along IN cord-022235-6ircruag 113 27 the the DT cord-022235-6ircruag 113 28 secretory secretory JJ cord-022235-6ircruag 113 29 pathway pathway NN cord-022235-6ircruag 113 30 , , , cord-022235-6ircruag 113 31 but but CC cord-022235-6ircruag 113 32 be be VB cord-022235-6ircruag 113 33 cause cause IN cord-022235-6ircruag 113 34 ATP ATP NNP cord-022235-6ircruag 113 35 is be VBZ cord-022235-6ircruag 113 36 usually usually RB cord-022235-6ircruag 113 37 present present JJ cord-022235-6ircruag 113 38 in in IN cord-022235-6ircruag 113 39 cell cell NN cord-022235-6ircruag 113 40 ly ly IN cord-022235-6ircruag 113 41 sates sates NNP cord-022235-6ircruag 113 42 , , , cord-022235-6ircruag 113 43 some some DT cord-022235-6ircruag 113 44 BiP BiP NNP cord-022235-6ircruag 113 45 complexes complex NNS cord-022235-6ircruag 113 46 ( ( -LRB- cord-022235-6ircruag 113 47 such such JJ cord-022235-6ircruag 113 48 as as IN cord-022235-6ircruag 113 49 G G NNP cord-022235-6ircruag 113 50 - - HYPH cord-022235-6ircruag 113 51 BiP BiP NNP cord-022235-6ircruag 113 52 ) ) -RRB- cord-022235-6ircruag 113 53 may may MD cord-022235-6ircruag 113 54 dissociate dissociate VB cord-022235-6ircruag 113 55 during during IN cord-022235-6ircruag 113 56 extraction extraction NN cord-022235-6ircruag 113 57 . . . cord-022235-6ircruag 114 1 BiP BiP NNP cord-022235-6ircruag 114 2 has have VBZ cord-022235-6ircruag 114 3 also also RB cord-022235-6ircruag 114 4 been be VBN cord-022235-6ircruag 114 5 shown show VBN cord-022235-6ircruag 114 6 to to TO cord-022235-6ircruag 114 7 associate associate VB cord-022235-6ircruag 114 8 with with IN cord-022235-6ircruag 114 9 nascent nascent JJ cord-022235-6ircruag 114 10 polypeptides polypeptide NNS cord-022235-6ircruag 114 11 as as IN cord-022235-6ircruag 114 12 they -PRON- PRP cord-022235-6ircruag 114 13 enter enter VBP cord-022235-6ircruag 114 14 the the DT cord-022235-6ircruag 114 15 lumen lumen NN cord-022235-6ircruag 114 16 of of IN cord-022235-6ircruag 114 17 the the DT cord-022235-6ircruag 114 18 ER ER NNP cord-022235-6ircruag 114 19 ( ( -LRB- cord-022235-6ircruag 114 20 557 557 CD cord-022235-6ircruag 114 21 ) ) -RRB- cord-022235-6ircruag 114 22 . . . cord-022235-6ircruag 115 1 Two two CD cord-022235-6ircruag 115 2 interesting interesting JJ cord-022235-6ircruag 115 3 features feature NNS cord-022235-6ircruag 115 4 of of IN cord-022235-6ircruag 115 5 BiP BiP NNP cord-022235-6ircruag 115 6 are be VBP cord-022235-6ircruag 115 7 that that IN cord-022235-6ircruag 115 8 its -PRON- PRP$ cord-022235-6ircruag 115 9 synthesis synthesis NN cord-022235-6ircruag 115 10 is be VBZ cord-022235-6ircruag 115 11 stimulated stimulate VBN cord-022235-6ircruag 115 12 by by IN cord-022235-6ircruag 115 13 glucose glucose NN cord-022235-6ircruag 115 14 deprivation deprivation NN cord-022235-6ircruag 115 15 and and CC cord-022235-6ircruag 115 16 that that IN cord-022235-6ircruag 115 17 it -PRON- PRP cord-022235-6ircruag 115 18 is be VBZ cord-022235-6ircruag 115 19 structurally structurally RB cord-022235-6ircruag 115 20 related relate VBN cord-022235-6ircruag 115 21 to to IN cord-022235-6ircruag 115 22 a a DT cord-022235-6ircruag 115 23 heat heat NN cord-022235-6ircruag 115 24 shock shock NN cord-022235-6ircruag 115 25 protein protein NN cord-022235-6ircruag 115 26 ( ( -LRB- cord-022235-6ircruag 115 27 758,857 758,857 CD cord-022235-6ircruag 115 28 ) ) -RRB- cord-022235-6ircruag 115 29 . . . cord-022235-6ircruag 116 1 Glucose glucose NN cord-022235-6ircruag 116 2 starvation starvation NN cord-022235-6ircruag 116 3 is be VBZ cord-022235-6ircruag 116 4 likely likely JJ cord-022235-6ircruag 116 5 to to TO cord-022235-6ircruag 116 6 reduce reduce VB cord-022235-6ircruag 116 7 glycosylation glycosylation NN cord-022235-6ircruag 116 8 , , , cord-022235-6ircruag 116 9 thereby thereby RB cord-022235-6ircruag 116 10 increasing increase VBG cord-022235-6ircruag 116 11 the the DT cord-022235-6ircruag 116 12 proportion proportion NN cord-022235-6ircruag 116 13 of of IN cord-022235-6ircruag 116 14 incorrectly incorrectly RB cord-022235-6ircruag 116 15 folded fold VBN cord-022235-6ircruag 116 16 secretory secretory JJ cord-022235-6ircruag 116 17 proteins protein NNS cord-022235-6ircruag 116 18 in in IN cord-022235-6ircruag 116 19 the the DT cord-022235-6ircruag 116 20 ER er NN cord-022235-6ircruag 116 21 . . . cord-022235-6ircruag 117 1 Studies study NNS cord-022235-6ircruag 117 2 have have VBP cord-022235-6ircruag 117 3 confirmed confirm VBN cord-022235-6ircruag 117 4 the the DT cord-022235-6ircruag 117 5 idea idea NN cord-022235-6ircruag 117 6 that that IN cord-022235-6ircruag 117 7 the the DT cord-022235-6ircruag 117 8 extent extent NN cord-022235-6ircruag 117 9 of of IN cord-022235-6ircruag 117 10 glycosylation glycosylation NN cord-022235-6ircruag 117 11 can can MD cord-022235-6ircruag 117 12 affect affect VB cord-022235-6ircruag 117 13 the the DT cord-022235-6ircruag 117 14 association association NN cord-022235-6ircruag 117 15 between between IN cord-022235-6ircruag 117 16 secretory secretory JJ cord-022235-6ircruag 117 17 proteins protein NNS cord-022235-6ircruag 117 18 and and CC cord-022235-6ircruag 117 19 BiP BiP NNP cord-022235-6ircruag 117 20 , , , cord-022235-6ircruag 117 21 as as RB cord-022235-6ircruag 117 22 well well RB cord-022235-6ircruag 117 23 as as IN cord-022235-6ircruag 117 24 their -PRON- PRP$ cord-022235-6ircruag 117 25 rate rate NN cord-022235-6ircruag 117 26 of of IN cord-022235-6ircruag 117 27 secretion secretion NN cord-022235-6ircruag 117 28 ( ( -LRB- cord-022235-6ircruag 117 29 251 251 CD cord-022235-6ircruag 117 30 ) ) -RRB- cord-022235-6ircruag 117 31 . . . cord-022235-6ircruag 118 1 The the DT cord-022235-6ircruag 118 2 accumulation accumulation NN cord-022235-6ircruag 118 3 of of IN cord-022235-6ircruag 118 4 misfolded misfolded JJ cord-022235-6ircruag 118 5 ( ( -LRB- cord-022235-6ircruag 118 6 mutant mutant JJ cord-022235-6ircruag 118 7 ) ) -RRB- cord-022235-6ircruag 118 8 pro pro JJ cord-022235-6ircruag 118 9 teins tein NNS cord-022235-6ircruag 118 10 in in IN cord-022235-6ircruag 118 11 the the DT cord-022235-6ircruag 118 12 ER ER NNP cord-022235-6ircruag 118 13 also also RB cord-022235-6ircruag 118 14 increases increase VBZ cord-022235-6ircruag 118 15 BiP BiP NNP cord-022235-6ircruag 118 16 synthesis synthesis NN cord-022235-6ircruag 118 17 ( ( -LRB- cord-022235-6ircruag 118 18 583 583 CD cord-022235-6ircruag 118 19 ) ) -RRB- cord-022235-6ircruag 118 20 , , , cord-022235-6ircruag 118 21 possibly possibly RB cord-022235-6ircruag 118 22 because because IN cord-022235-6ircruag 118 23 the the DT cord-022235-6ircruag 118 24 cell cell NN cord-022235-6ircruag 118 25 senses sense VBZ cord-022235-6ircruag 118 26 that that IN cord-022235-6ircruag 118 27 its -PRON- PRP$ cord-022235-6ircruag 118 28 store store NN cord-022235-6ircruag 118 29 of of IN cord-022235-6ircruag 118 30 BiP BiP NNP cord-022235-6ircruag 118 31 has have VBZ cord-022235-6ircruag 118 32 been be VBN cord-022235-6ircruag 118 33 sequestered sequester VBN cord-022235-6ircruag 118 34 into into IN cord-022235-6ircruag 118 35 protein protein NN cord-022235-6ircruag 118 36 com com NN cord-022235-6ircruag 118 37 plexes plexe NNS cord-022235-6ircruag 118 38 . . . cord-022235-6ircruag 119 1 Thus thus RB cord-022235-6ircruag 119 2 , , , cord-022235-6ircruag 119 3 synthesis synthesis NN cord-022235-6ircruag 119 4 of of IN cord-022235-6ircruag 119 5 BiP BiP NNP cord-022235-6ircruag 119 6 may may MD cord-022235-6ircruag 119 7 be be VB cord-022235-6ircruag 119 8 increased increase VBN cord-022235-6ircruag 119 9 according accord VBG cord-022235-6ircruag 119 10 to to TO cord-022235-6ircruag 119 11 require require VB cord-022235-6ircruag 119 12 ments ment NNS cord-022235-6ircruag 119 13 ( ( -LRB- cord-022235-6ircruag 119 14 857 857 CD cord-022235-6ircruag 119 15 ) ) -RRB- cord-022235-6ircruag 119 16 , , , cord-022235-6ircruag 119 17 but but CC cord-022235-6ircruag 119 18 studies study NNS cord-022235-6ircruag 119 19 on on IN cord-022235-6ircruag 119 20 the the DT cord-022235-6ircruag 119 21 " " `` cord-022235-6ircruag 119 22 proofreading proofreading NN cord-022235-6ircruag 119 23 " " '' cord-022235-6ircruag 119 24 or or CC cord-022235-6ircruag 119 25 quality quality NN cord-022235-6ircruag 119 26 control control NN cord-022235-6ircruag 119 27 role role NN cord-022235-6ircruag 119 28 of of IN cord-022235-6ircruag 119 29 BiP BiP NNP cord-022235-6ircruag 119 30 are be VBP cord-022235-6ircruag 119 31 still still RB cord-022235-6ircruag 119 32 at at IN cord-022235-6ircruag 119 33 an an DT cord-022235-6ircruag 119 34 early early JJ cord-022235-6ircruag 119 35 stage stage NN cord-022235-6ircruag 119 36 . . . cord-022235-6ircruag 120 1 A a DT cord-022235-6ircruag 120 2 different different JJ cord-022235-6ircruag 120 3 , , , cord-022235-6ircruag 120 4 cytoplasmic cytoplasmic JJ cord-022235-6ircruag 120 5 quality quality NN cord-022235-6ircruag 120 6 control control NN cord-022235-6ircruag 120 7 protein protein NN cord-022235-6ircruag 120 8 may may MD cord-022235-6ircruag 120 9 be be VB cord-022235-6ircruag 120 10 responsible responsible JJ cord-022235-6ircruag 120 11 for for IN cord-022235-6ircruag 120 12 proofreading proofread VBG cord-022235-6ircruag 120 13 cytoplasmic cytoplasmic JJ cord-022235-6ircruag 120 14 domains domain NNS cord-022235-6ircruag 120 15 in in IN cord-022235-6ircruag 120 16 transmembrane transmembrane NN cord-022235-6ircruag 120 17 proteins protein NNS cord-022235-6ircruag 120 18 to to TO cord-022235-6ircruag 120 19 ensure ensure VB cord-022235-6ircruag 120 20 that that IN cord-022235-6ircruag 120 21 they -PRON- PRP cord-022235-6ircruag 120 22 too too RB cord-022235-6ircruag 120 23 are be VBP cord-022235-6ircruag 120 24 correctly correctly RB cord-022235-6ircruag 120 25 folded fold VBN cord-022235-6ircruag 120 26 and and CC cord-022235-6ircruag 120 27 oligomerized oligomerize VBN cord-022235-6ircruag 120 28 ( ( -LRB- cord-022235-6ircruag 120 29 406a 406a CD cord-022235-6ircruag 120 30 ) ) -RRB- cord-022235-6ircruag 120 31 . . . cord-022235-6ircruag 121 1 Protein protein NN cord-022235-6ircruag 121 2 conformation conformation NN cord-022235-6ircruag 121 3 , , , cord-022235-6ircruag 121 4 rather rather RB cord-022235-6ircruag 121 5 than than IN cord-022235-6ircruag 121 6 any any DT cord-022235-6ircruag 121 7 specific specific JJ cord-022235-6ircruag 121 8 structural structural JJ cord-022235-6ircruag 121 9 feature feature NN cord-022235-6ircruag 121 10 such such JJ cord-022235-6ircruag 121 11 as as IN cord-022235-6ircruag 121 12 glycosylated glycosylated JJ cord-022235-6ircruag 121 13 residues residue NNS cord-022235-6ircruag 121 14 , , , cord-022235-6ircruag 121 15 is be VBZ cord-022235-6ircruag 121 16 thus thus RB cord-022235-6ircruag 121 17 likely likely JJ cord-022235-6ircruag 121 18 to to TO cord-022235-6ircruag 121 19 determine determine VB cord-022235-6ircruag 121 20 whether whether IN cord-022235-6ircruag 121 21 a a DT cord-022235-6ircruag 121 22 protein protein NN cord-022235-6ircruag 121 23 is be VBZ cord-022235-6ircruag 121 24 competent competent JJ cord-022235-6ircruag 121 25 to to TO cord-022235-6ircruag 121 26 leave leave VB cord-022235-6ircruag 121 27 the the DT cord-022235-6ircruag 121 28 ER er NN cord-022235-6ircruag 121 29 . . . cord-022235-6ircruag 122 1 This this DT cord-022235-6ircruag 122 2 feature feature NN cord-022235-6ircruag 122 3 is be VBZ cord-022235-6ircruag 122 4 illustrated illustrate VBN cord-022235-6ircruag 122 5 by by IN cord-022235-6ircruag 122 6 studies study NNS cord-022235-6ircruag 122 7 on on IN cord-022235-6ircruag 122 8 the the DT cord-022235-6ircruag 122 9 effects effect NNS cord-022235-6ircruag 122 10 of of IN cord-022235-6ircruag 122 11 glycosylation glycosylation NN cord-022235-6ircruag 122 12 on on IN cord-022235-6ircruag 122 13 VSV VSV NNP cord-022235-6ircruag 122 14 G G NNP cord-022235-6ircruag 122 15 protein protein NN cord-022235-6ircruag 122 16 . . . cord-022235-6ircruag 123 1 Machamer Machamer NNP cord-022235-6ircruag 123 2 et et NNP cord-022235-6ircruag 123 3 al al NNP cord-022235-6ircruag 123 4 . . . cord-022235-6ircruag 124 1 ( ( -LRB- cord-022235-6ircruag 124 2 672 672 CD cord-022235-6ircruag 124 3 ) ) -RRB- cord-022235-6ircruag 124 4 used use VBN cord-022235-6ircruag 124 5 site site NN cord-022235-6ircruag 124 6 - - HYPH cord-022235-6ircruag 124 7 directed direct VBN cord-022235-6ircruag 124 8 mutagenesis mutagenesis NN cord-022235-6ircruag 124 9 to to TO cord-022235-6ircruag 124 10 replace replace VB cord-022235-6ircruag 124 11 the the DT cord-022235-6ircruag 124 12 glycosylated glycosylate VBN cord-022235-6ircruag 124 13 Asn Asn NNP cord-022235-6ircruag 124 14 residues residue NNS cord-022235-6ircruag 124 15 . . . cord-022235-6ircruag 125 1 Nonglycosylated Nonglycosylated NNP cord-022235-6ircruag 125 2 G G NNP cord-022235-6ircruag 125 3 protein protein NN cord-022235-6ircruag 125 4 was be VBD cord-022235-6ircruag 125 5 transported transport VBN cord-022235-6ircruag 125 6 from from IN cord-022235-6ircruag 125 7 the the DT cord-022235-6ircruag 125 8 ER ER NNP cord-022235-6ircruag 125 9 to to IN cord-022235-6ircruag 125 10 the the DT cord-022235-6ircruag 125 11 Golgi Golgi NNP cord-022235-6ircruag 125 12 but but CC cord-022235-6ircruag 125 13 did do VBD cord-022235-6ircruag 125 14 not not RB cord-022235-6ircruag 125 15 reach reach VB cord-022235-6ircruag 125 16 the the DT cord-022235-6ircruag 125 17 cell cell NN cord-022235-6ircruag 125 18 surface surface NN cord-022235-6ircruag 125 19 . . . cord-022235-6ircruag 126 1 When when WRB cord-022235-6ircruag 126 2 only only RB cord-022235-6ircruag 126 3 one one CD cord-022235-6ircruag 126 4 of of IN cord-022235-6ircruag 126 5 the the DT cord-022235-6ircruag 126 6 Asn Asn NNP cord-022235-6ircruag 126 7 residues residue NNS cord-022235-6ircruag 126 8 was be VBD cord-022235-6ircruag 126 9 de de FW cord-022235-6ircruag 126 10 leted leted NNP cord-022235-6ircruag 126 11 , , , cord-022235-6ircruag 126 12 the the DT cord-022235-6ircruag 126 13 protein protein NN cord-022235-6ircruag 126 14 reached reach VBD cord-022235-6ircruag 126 15 the the DT cord-022235-6ircruag 126 16 cell cell NN cord-022235-6ircruag 126 17 surface surface NN cord-022235-6ircruag 126 18 ; ; : cord-022235-6ircruag 126 19 thus thus RB cord-022235-6ircruag 126 20 G g NN cord-022235-6ircruag 126 21 protein protein NN cord-022235-6ircruag 126 22 with with IN cord-022235-6ircruag 126 23 one one CD cord-022235-6ircruag 126 24 glyco glyco NNP cord-022235-6ircruag 126 25 sylated sylate VBD cord-022235-6ircruag 126 26 Asn Asn NNP cord-022235-6ircruag 126 27 residue residue NN cord-022235-6ircruag 126 28 can can MD cord-022235-6ircruag 126 29 be be VB cord-022235-6ircruag 126 30 transported transport VBN cord-022235-6ircruag 126 31 through through IN cord-022235-6ircruag 126 32 the the DT cord-022235-6ircruag 126 33 entire entire JJ cord-022235-6ircruag 126 34 secretory secretory JJ cord-022235-6ircruag 126 35 path path NN cord-022235-6ircruag 126 36 way way NN cord-022235-6ircruag 126 37 . . . cord-022235-6ircruag 127 1 G g NN cord-022235-6ircruag 127 2 protein protein NN cord-022235-6ircruag 127 3 export export NN cord-022235-6ircruag 127 4 became become VBD cord-022235-6ircruag 127 5 temperature temperature NN cord-022235-6ircruag 127 6 - - HYPH cord-022235-6ircruag 127 7 sensitive sensitive JJ cord-022235-6ircruag 127 8 when when WRB cord-022235-6ircruag 127 9 new new JJ cord-022235-6ircruag 127 10 glycosylation glycosylation NN cord-022235-6ircruag 127 11 sites site NNS cord-022235-6ircruag 127 12 were be VBD cord-022235-6ircruag 127 13 added add VBN cord-022235-6ircruag 127 14 ( ( -LRB- cord-022235-6ircruag 127 15 671 671 CD cord-022235-6ircruag 127 16 ) ) -RRB- cord-022235-6ircruag 127 17 . . . cord-022235-6ircruag 128 1 Kotwal Kotwal NNP cord-022235-6ircruag 128 2 et et NNP cord-022235-6ircruag 128 3 al al NNP cord-022235-6ircruag 128 4 . . . cord-022235-6ircruag 129 1 ( ( -LRB- cord-022235-6ircruag 129 2 581 581 CD cord-022235-6ircruag 129 3 ) ) -RRB- cord-022235-6ircruag 129 4 noted note VBD cord-022235-6ircruag 129 5 that that IN cord-022235-6ircruag 129 6 some some DT cord-022235-6ircruag 129 7 natural natural JJ cord-022235-6ircruag 129 8 G g NN cord-022235-6ircruag 129 9 protein protein NN cord-022235-6ircruag 129 10 variants variant NNS cord-022235-6ircruag 129 11 have have VBP cord-022235-6ircruag 129 12 only only RB cord-022235-6ircruag 129 13 one one CD cord-022235-6ircruag 129 14 oligosaccharide oligosaccharide NN cord-022235-6ircruag 129 15 whereas whereas IN cord-022235-6ircruag 129 16 others other NNS cord-022235-6ircruag 129 17 have have VBP cord-022235-6ircruag 129 18 none none NN cord-022235-6ircruag 129 19 at at RB cord-022235-6ircruag 129 20 all all RB cord-022235-6ircruag 129 21 and and CC cord-022235-6ircruag 129 22 suggested suggest VBD cord-022235-6ircruag 129 23 that that IN cord-022235-6ircruag 129 24 compensatory compensatory JJ cord-022235-6ircruag 129 25 changes change NNS cord-022235-6ircruag 129 26 in in IN cord-022235-6ircruag 129 27 primary primary JJ cord-022235-6ircruag 129 28 structure structure NN cord-022235-6ircruag 129 29 may may MD cord-022235-6ircruag 129 30 allow allow VB cord-022235-6ircruag 129 31 nonglycosylated nonglycosylated JJ cord-022235-6ircruag 129 32 protein protein NN cord-022235-6ircruag 129 33 to to TO cord-022235-6ircruag 129 34 fold fold VB cord-022235-6ircruag 129 35 into into IN cord-022235-6ircruag 129 36 a a DT cord-022235-6ircruag 129 37 secretion secretion NN cord-022235-6ircruag 129 38 - - HYPH cord-022235-6ircruag 129 39 com com NN cord-022235-6ircruag 129 40 petent petent NN cord-022235-6ircruag 129 41 conformation conformation NN cord-022235-6ircruag 129 42 . . . cord-022235-6ircruag 130 1 Thus thus RB cord-022235-6ircruag 130 2 , , , cord-022235-6ircruag 130 3 glycosylation glycosylation NN cord-022235-6ircruag 130 4 is be VBZ cord-022235-6ircruag 130 5 probably probably RB cord-022235-6ircruag 130 6 needed need VBN cord-022235-6ircruag 130 7 for for IN cord-022235-6ircruag 130 8 correct correct JJ cord-022235-6ircruag 130 9 protein protein NN cord-022235-6ircruag 130 10 folding folding NN cord-022235-6ircruag 130 11 but but CC cord-022235-6ircruag 130 12 is be VBZ cord-022235-6ircruag 130 13 not not RB cord-022235-6ircruag 130 14 directly directly RB cord-022235-6ircruag 130 15 implicated implicate VBN cord-022235-6ircruag 130 16 in in IN cord-022235-6ircruag 130 17 the the DT cord-022235-6ircruag 130 18 formation formation NN cord-022235-6ircruag 130 19 of of IN cord-022235-6ircruag 130 20 secretion secretion NN cord-022235-6ircruag 130 21 competence competence NN cord-022235-6ircruag 130 22 signals signal NNS cord-022235-6ircruag 130 23 . . . cord-022235-6ircruag 131 1 At at RB cord-022235-6ircruag 131 2 least least RBS cord-022235-6ircruag 131 3 one one CD cord-022235-6ircruag 131 4 secreted secrete VBN cord-022235-6ircruag 131 5 protein protein NN cord-022235-6ircruag 131 6 , , , cord-022235-6ircruag 131 7 ovalbumin ovalbumin NNP cord-022235-6ircruag 131 8 , , , cord-022235-6ircruag 131 9 is be VBZ cord-022235-6ircruag 131 10 not not RB cord-022235-6ircruag 131 11 gly gly NNP cord-022235-6ircruag 131 12 cosylated cosylate VBN cord-022235-6ircruag 131 13 . . . cord-022235-6ircruag 132 1 The the DT cord-022235-6ircruag 132 2 ER ER NNP cord-022235-6ircruag 132 3 contains contain VBZ cord-022235-6ircruag 132 4 a a DT cord-022235-6ircruag 132 5 large large JJ cord-022235-6ircruag 132 6 number number NN cord-022235-6ircruag 132 7 of of IN cord-022235-6ircruag 132 8 proteins protein NNS cord-022235-6ircruag 132 9 , , , cord-022235-6ircruag 132 10 some some DT cord-022235-6ircruag 132 11 of of IN cord-022235-6ircruag 132 12 which which WDT cord-022235-6ircruag 132 13 it -PRON- PRP cord-022235-6ircruag 132 14 shares share VBZ cord-022235-6ircruag 132 15 with with IN cord-022235-6ircruag 132 16 the the DT cord-022235-6ircruag 132 17 contiguous contiguous JJ cord-022235-6ircruag 132 18 nuclear nuclear JJ cord-022235-6ircruag 132 19 membrane membrane NN cord-022235-6ircruag 132 20 ( ( -LRB- cord-022235-6ircruag 132 21 117 117 CD cord-022235-6ircruag 132 22 ) ) -RRB- cord-022235-6ircruag 132 23 . . . cord-022235-6ircruag 133 1 They -PRON- PRP cord-022235-6ircruag 133 2 include include VBP cord-022235-6ircruag 133 3 proteins protein NNS cord-022235-6ircruag 133 4 involved involve VBN cord-022235-6ircruag 133 5 in in IN cord-022235-6ircruag 133 6 secretory secretory JJ cord-022235-6ircruag 133 7 protein protein NN cord-022235-6ircruag 133 8 translocation translocation NN cord-022235-6ircruag 133 9 through through IN cord-022235-6ircruag 133 10 the the DT cord-022235-6ircruag 133 11 RERM RERM NNP cord-022235-6ircruag 133 12 , , , cord-022235-6ircruag 133 13 signal signal NN cord-022235-6ircruag 133 14 peptidase peptidase NN cord-022235-6ircruag 133 15 , , , cord-022235-6ircruag 133 16 ribophorins ribophorin NNS cord-022235-6ircruag 133 17 , , , cord-022235-6ircruag 133 18 BiP BiP NNP cord-022235-6ircruag 133 19 , , , cord-022235-6ircruag 133 20 enzymes enzyme NNS cord-022235-6ircruag 133 21 involved involve VBN cord-022235-6ircruag 133 22 in in IN cord-022235-6ircruag 133 23 lipid lipid NN cord-022235-6ircruag 133 24 synthesis synthesis NN cord-022235-6ircruag 133 25 and and CC cord-022235-6ircruag 133 26 in in IN cord-022235-6ircruag 133 27 protein protein NN cord-022235-6ircruag 133 28 glycosylation glycosylation NN cord-022235-6ircruag 133 29 , , , cord-022235-6ircruag 133 30 and and CC cord-022235-6ircruag 133 31 cytochrome cytochrome NN cord-022235-6ircruag 133 32 P p NN cord-022235-6ircruag 133 33 4 4 CD cord-022235-6ircruag 133 34 5 5 CD cord-022235-6ircruag 133 35 0 0 CD cord-022235-6ircruag 133 36 . . . cord-022235-6ircruag 134 1 The the DT cord-022235-6ircruag 134 2 characterized characterize VBN cord-022235-6ircruag 134 3 proteins protein NNS cord-022235-6ircruag 134 4 are be VBP cord-022235-6ircruag 134 5 similar similar JJ cord-022235-6ircruag 134 6 to to IN cord-022235-6ircruag 134 7 other other JJ cord-022235-6ircruag 134 8 secretory secretory JJ cord-022235-6ircruag 134 9 proteins protein NNS cord-022235-6ircruag 134 10 : : : cord-022235-6ircruag 134 11 They -PRON- PRP cord-022235-6ircruag 134 12 often often RB cord-022235-6ircruag 134 13 have have VBP cord-022235-6ircruag 134 14 signal signal NN cord-022235-6ircruag 134 15 peptides peptide NNS cord-022235-6ircruag 134 16 ( ( -LRB- cord-022235-6ircruag 134 17 198 198 CD cord-022235-6ircruag 134 18 , , , cord-022235-6ircruag 134 19 630 630 CD cord-022235-6ircruag 134 20 , , , cord-022235-6ircruag 134 21 1089 1089 CD cord-022235-6ircruag 134 22 ) ) -RRB- cord-022235-6ircruag 134 23 , , , cord-022235-6ircruag 134 24 are be VBP cord-022235-6ircruag 134 25 glycosylated glycosylate VBN cord-022235-6ircruag 134 26 , , , cord-022235-6ircruag 134 27 and and CC cord-022235-6ircruag 134 28 may may MD cord-022235-6ircruag 134 29 be be VB cord-022235-6ircruag 134 30 located locate VBN cord-022235-6ircruag 134 31 in in IN cord-022235-6ircruag 134 32 the the DT cord-022235-6ircruag 134 33 lumen lumen NN cord-022235-6ircruag 134 34 or or CC cord-022235-6ircruag 134 35 in in IN cord-022235-6ircruag 134 36 the the DT cord-022235-6ircruag 134 37 RERM RERM NNP cord-022235-6ircruag 134 38 . . . cord-022235-6ircruag 135 1 Rothman Rothman NNP cord-022235-6ircruag 135 2 ( ( -LRB- cord-022235-6ircruag 135 3 965 965 CD cord-022235-6ircruag 135 4 ) ) -RRB- cord-022235-6ircruag 135 5 has have VBZ cord-022235-6ircruag 135 6 argued argue VBN cord-022235-6ircruag 135 7 that that IN cord-022235-6ircruag 135 8 it -PRON- PRP cord-022235-6ircruag 135 9 may may MD cord-022235-6ircruag 135 10 be be VB cord-022235-6ircruag 135 11 physically physically RB cord-022235-6ircruag 135 12 impossible impossible JJ cord-022235-6ircruag 135 13 for for IN cord-022235-6ircruag 135 14 the the DT cord-022235-6ircruag 135 15 ER ER NNP cord-022235-6ircruag 135 16 to to TO cord-022235-6ircruag 135 17 prevent prevent VB cord-022235-6ircruag 135 18 the the DT cord-022235-6ircruag 135 19 escape escape NN cord-022235-6ircruag 135 20 of of IN cord-022235-6ircruag 135 21 endogenous endogenous JJ cord-022235-6ircruag 135 22 proteins protein NNS cord-022235-6ircruag 135 23 , , , cord-022235-6ircruag 135 24 and and CC cord-022235-6ircruag 135 25 particularly particularly RB cord-022235-6ircruag 135 26 mem mem NNP cord-022235-6ircruag 135 27 brane brane NN cord-022235-6ircruag 135 28 proteins protein NNS cord-022235-6ircruag 135 29 , , , cord-022235-6ircruag 135 30 to to IN cord-022235-6ircruag 135 31 the the DT cord-022235-6ircruag 135 32 cis cis NNP cord-022235-6ircruag 135 33 Golgi Golgi NNP cord-022235-6ircruag 135 34 . . . cord-022235-6ircruag 136 1 He -PRON- PRP cord-022235-6ircruag 136 2 cited cite VBD cord-022235-6ircruag 136 3 two two CD cord-022235-6ircruag 136 4 observations observation NNS cord-022235-6ircruag 136 5 in in IN cord-022235-6ircruag 136 6 support support NN cord-022235-6ircruag 136 7 of of IN cord-022235-6ircruag 136 8 the the DT cord-022235-6ircruag 136 9 idea idea NN cord-022235-6ircruag 136 10 that that IN cord-022235-6ircruag 136 11 these these DT cord-022235-6ircruag 136 12 proteins protein NNS cord-022235-6ircruag 136 13 could could MD cord-022235-6ircruag 136 14 leave leave VB cord-022235-6ircruag 136 15 the the DT cord-022235-6ircruag 136 16 RER RER NNP cord-022235-6ircruag 136 17 as as IN cord-022235-6ircruag 136 18 part part NN cord-022235-6ircruag 136 19 of of IN cord-022235-6ircruag 136 20 the the DT cord-022235-6ircruag 136 21 bulk bulk JJ cord-022235-6ircruag 136 22 flow flow NN cord-022235-6ircruag 136 23 through through IN cord-022235-6ircruag 136 24 the the DT cord-022235-6ircruag 136 25 secretory secretory JJ cord-022235-6ircruag 136 26 pathway pathway NN cord-022235-6ircruag 136 27 , , , cord-022235-6ircruag 136 28 and and CC cord-022235-6ircruag 136 29 then then RB cord-022235-6ircruag 136 30 recycle recycle VB cord-022235-6ircruag 136 31 from from IN cord-022235-6ircruag 136 32 the the DT cord-022235-6ircruag 136 33 Golgi Golgi NNP cord-022235-6ircruag 136 34 to to IN cord-022235-6ircruag 136 35 the the DT cord-022235-6ircruag 136 36 ER er NN cord-022235-6ircruag 136 37 : : : cord-022235-6ircruag 136 38 ( ( -LRB- cord-022235-6ircruag 136 39 i i NN cord-022235-6ircruag 136 40 ) ) -RRB- cord-022235-6ircruag 137 1 Some some DT cord-022235-6ircruag 137 2 ER ER NNP cord-022235-6ircruag 137 3 proteins protein NNS cord-022235-6ircruag 137 4 are be VBP cord-022235-6ircruag 137 5 detected detect VBN cord-022235-6ircruag 137 6 in in IN cord-022235-6ircruag 137 7 significant significant JJ cord-022235-6ircruag 137 8 amounts amount NNS cord-022235-6ircruag 137 9 in in IN cord-022235-6ircruag 137 10 vesicles vesicle NNS cord-022235-6ircruag 137 11 derived derive VBN cord-022235-6ircruag 137 12 from from IN cord-022235-6ircruag 137 13 the the DT cord-022235-6ircruag 137 14 cis cis NNP cord-022235-6ircruag 137 15 Golgi Golgi NNP cord-022235-6ircruag 137 16 when when WRB cord-022235-6ircruag 137 17 cells cell NNS cord-022235-6ircruag 137 18 are be VBP cord-022235-6ircruag 137 19 fractionated fractionate VBN cord-022235-6ircruag 137 20 ( ( -LRB- cord-022235-6ircruag 137 21 102 102 CD cord-022235-6ircruag 137 22 ) ) -RRB- cord-022235-6ircruag 137 23 . . . cord-022235-6ircruag 138 1 ( ( -LRB- cord-022235-6ircruag 138 2 ii ii NN cord-022235-6ircruag 138 3 ) ) -RRB- cord-022235-6ircruag 139 1 Bulk bulk JJ cord-022235-6ircruag 139 2 lipid lipid NN cord-022235-6ircruag 139 3 flow flow NN cord-022235-6ircruag 139 4 out out IN cord-022235-6ircruag 139 5 of of IN cord-022235-6ircruag 139 6 the the DT cord-022235-6ircruag 139 7 ER ER NNP cord-022235-6ircruag 139 8 necessitates necessitate VBZ cord-022235-6ircruag 139 9 efficient efficient JJ cord-022235-6ircruag 139 10 recycling recycling NN cord-022235-6ircruag 139 11 , , , cord-022235-6ircruag 139 12 possi possi NNP cord-022235-6ircruag 139 13 bly bly NNP cord-022235-6ircruag 139 14 from from IN cord-022235-6ircruag 139 15 the the DT cord-022235-6ircruag 139 16 cis cis NNP cord-022235-6ircruag 139 17 Golgi Golgi NNP cord-022235-6ircruag 139 18 , , , cord-022235-6ircruag 139 19 which which WDT cord-022235-6ircruag 139 20 could could MD cord-022235-6ircruag 139 21 provide provide VB cord-022235-6ircruag 139 22 " " `` cord-022235-6ircruag 139 23 carriers carrier NNS cord-022235-6ircruag 139 24 " " '' cord-022235-6ircruag 139 25 for for IN cord-022235-6ircruag 139 26 the the DT cord-022235-6ircruag 139 27 recy recy NN cord-022235-6ircruag 139 28 cling cling VB cord-022235-6ircruag 139 29 of of IN cord-022235-6ircruag 139 30 ER ER NNP cord-022235-6ircruag 139 31 proteins protein NNS cord-022235-6ircruag 139 32 . . . cord-022235-6ircruag 140 1 Most Most JJS cord-022235-6ircruag 140 2 ER ER NNP cord-022235-6ircruag 140 3 proteins protein NNS cord-022235-6ircruag 140 4 are be VBP cord-022235-6ircruag 140 5 , , , cord-022235-6ircruag 140 6 however however RB cord-022235-6ircruag 140 7 , , , cord-022235-6ircruag 140 8 almost almost RB cord-022235-6ircruag 140 9 completely completely RB cord-022235-6ircruag 140 10 excluded exclude VBN cord-022235-6ircruag 140 11 from from IN cord-022235-6ircruag 140 12 the the DT cord-022235-6ircruag 140 13 Golgi Golgi NNP cord-022235-6ircruag 140 14 ( ( -LRB- cord-022235-6ircruag 140 15 663,697 663,697 CD cord-022235-6ircruag 140 16 ) ) -RRB- cord-022235-6ircruag 140 17 . . . cord-022235-6ircruag 141 1 Furthermore furthermore RB cord-022235-6ircruag 141 2 , , , cord-022235-6ircruag 141 3 ER ER NNP cord-022235-6ircruag 141 4 proteins protein NNS cord-022235-6ircruag 141 5 are be VBP cord-022235-6ircruag 141 6 not not RB cord-022235-6ircruag 141 7 terminally terminally RB cord-022235-6ircruag 141 8 glycosyla glycosyla NNP cord-022235-6ircruag 141 9 ted ted NNP cord-022235-6ircruag 141 10 ( ( -LRB- cord-022235-6ircruag 141 11 117,1089 117,1089 NNP cord-022235-6ircruag 141 12 ) ) -RRB- cord-022235-6ircruag 141 13 , , , cord-022235-6ircruag 141 14 which which WDT cord-022235-6ircruag 141 15 means mean VBZ cord-022235-6ircruag 141 16 that that IN cord-022235-6ircruag 141 17 they -PRON- PRP cord-022235-6ircruag 141 18 do do VBP cord-022235-6ircruag 141 19 not not RB cord-022235-6ircruag 141 20 reach reach VB cord-022235-6ircruag 141 21 the the DT cord-022235-6ircruag 141 22 medial medial JJ cord-022235-6ircruag 141 23 or or CC cord-022235-6ircruag 141 24 trans trans JJ cord-022235-6ircruag 141 25 Golgi Golgi NNP cord-022235-6ircruag 141 26 ( ( -LRB- cord-022235-6ircruag 141 27 Section section NN cord-022235-6ircruag 141 28 V.E V.E NNP cord-022235-6ircruag 141 29 ) ) -RRB- cord-022235-6ircruag 141 30 . . . cord-022235-6ircruag 142 1 Mannose mannose NN cord-022235-6ircruag 142 2 residues residue NNS cord-022235-6ircruag 142 3 on on IN cord-022235-6ircruag 142 4 some some DT cord-022235-6ircruag 142 5 ER er NN cord-022235-6ircruag 142 6 proteins protein NNS cord-022235-6ircruag 142 7 are be VBP cord-022235-6ircruag 142 8 not not RB cord-022235-6ircruag 142 9 trimmed trim VBN cord-022235-6ircruag 142 10 beyond beyond IN cord-022235-6ircruag 142 11 the the DT cord-022235-6ircruag 142 12 stage stage NN cord-022235-6ircruag 142 13 catalyzed catalyze VBN cord-022235-6ircruag 142 14 by by IN cord-022235-6ircruag 142 15 ER ER NNP cord-022235-6ircruag 142 16 mannosidase mannosidase NNP cord-022235-6ircruag 142 17 ( ( -LRB- cord-022235-6ircruag 142 18 117,954,1089 117,954,1089 CD cord-022235-6ircruag 142 19 ) ) -RRB- cord-022235-6ircruag 142 20 , , , cord-022235-6ircruag 142 21 but but CC cord-022235-6ircruag 142 22 a a DT cord-022235-6ircruag 142 23 lysosomal lysosomal JJ cord-022235-6ircruag 142 24 protein protein NN cord-022235-6ircruag 142 25 carrying carry VBG cord-022235-6ircruag 142 26 an an DT cord-022235-6ircruag 142 27 ER ER NNP cord-022235-6ircruag 142 28 retention retention NN cord-022235-6ircruag 142 29 signal signal NN cord-022235-6ircruag 142 30 ( ( -LRB- cord-022235-6ircruag 142 31 see see VB cord-022235-6ircruag 142 32 below below RB cord-022235-6ircruag 142 33 ) ) -RRB- cord-022235-6ircruag 142 34 is be VBZ cord-022235-6ircruag 142 35 phosphorylated phosphorylate VBN cord-022235-6ircruag 142 36 by by IN cord-022235-6ircruag 142 37 NAGn NAGn NNP cord-022235-6ircruag 142 38 phosphotransferase phosphotransferase NNP cord-022235-6ircruag 142 39 , , , cord-022235-6ircruag 142 40 indicating indicate VBG cord-022235-6ircruag 142 41 that that IN cord-022235-6ircruag 142 42 it -PRON- PRP cord-022235-6ircruag 142 43 reaches reach VBZ cord-022235-6ircruag 142 44 the the DT cord-022235-6ircruag 142 45 early early JJ cord-022235-6ircruag 142 46 cis cis NNP cord-022235-6ircruag 142 47 Golgi Golgi NNP cord-022235-6ircruag 142 48 ( ( -LRB- cord-022235-6ircruag 142 49 858 858 CD cord-022235-6ircruag 142 50 ) ) -RRB- cord-022235-6ircruag 142 51 . . . cord-022235-6ircruag 143 1 Significantly significantly RB cord-022235-6ircruag 143 2 , , , cord-022235-6ircruag 143 3 the the DT cord-022235-6ircruag 143 4 phosphate phosphate NN cord-022235-6ircruag 143 5 groups group NNS cord-022235-6ircruag 143 6 are be VBP cord-022235-6ircruag 143 7 not not RB cord-022235-6ircruag 143 8 modi modi NNP cord-022235-6ircruag 143 9 fied fie VBN cord-022235-6ircruag 143 10 by by IN cord-022235-6ircruag 143 11 NAGn NAGn NNP cord-022235-6ircruag 143 12 phosphodiesterase phosphodiesterase NNP cord-022235-6ircruag 143 13 , , , cord-022235-6ircruag 143 14 which which WDT cord-022235-6ircruag 143 15 may may MD cord-022235-6ircruag 143 16 be be VB cord-022235-6ircruag 143 17 located locate VBN cord-022235-6ircruag 143 18 in in IN cord-022235-6ircruag 143 19 a a DT cord-022235-6ircruag 143 20 different different JJ cord-022235-6ircruag 143 21 , , , cord-022235-6ircruag 143 22 ER ER NNP cord-022235-6ircruag 143 23 - - HYPH cord-022235-6ircruag 143 24 distal distal JJ cord-022235-6ircruag 143 25 Golgi Golgi NNP cord-022235-6ircruag 143 26 compartment compartment NN cord-022235-6ircruag 143 27 ( ( -LRB- cord-022235-6ircruag 143 28 Table table NN cord-022235-6ircruag 143 29 V.l v.l NN cord-022235-6ircruag 143 30 ) ) -RRB- cord-022235-6ircruag 143 31 . . . cord-022235-6ircruag 144 1 Warren Warren NNP cord-022235-6ircruag 144 2 ( ( -LRB- cord-022235-6ircruag 144 3 1166 1166 CD cord-022235-6ircruag 144 4 ) ) -RRB- cord-022235-6ircruag 144 5 has have VBZ cord-022235-6ircruag 144 6 proposed propose VBN cord-022235-6ircruag 144 7 that that IN cord-022235-6ircruag 144 8 ER ER NNP cord-022235-6ircruag 144 9 proteins protein NNS cord-022235-6ircruag 144 10 are be VBP cord-022235-6ircruag 144 11 salvaged salvage VBN cord-022235-6ircruag 144 12 from from IN cord-022235-6ircruag 144 13 an an DT cord-022235-6ircruag 144 14 intermediate intermediate JJ cord-022235-6ircruag 144 15 compartment compartment NN cord-022235-6ircruag 144 16 , , , cord-022235-6ircruag 144 17 the the DT cord-022235-6ircruag 144 18 socalled socalled JJ cord-022235-6ircruag 144 19 transitional transitional JJ cord-022235-6ircruag 144 20 element element NN cord-022235-6ircruag 144 21 ( ( -LRB- cord-022235-6ircruag 144 22 Section section NN cord-022235-6ircruag 144 23 V.C V.C NNP cord-022235-6ircruag 144 24 ) ) -RRB- cord-022235-6ircruag 144 25 , , , cord-022235-6ircruag 144 26 located locate VBN cord-022235-6ircruag 144 27 between between IN cord-022235-6ircruag 144 28 the the DT cord-022235-6ircruag 144 29 ER ER NNP cord-022235-6ircruag 144 30 and and CC cord-022235-6ircruag 144 31 the the DT cord-022235-6ircruag 144 32 cis cis NNP cord-022235-6ircruag 144 33 Golgi Golgi NNP cord-022235-6ircruag 144 34 . . . cord-022235-6ircruag 145 1 Recycling recycling NN cord-022235-6ircruag 145 2 of of IN cord-022235-6ircruag 145 3 " " `` cord-022235-6ircruag 145 4 escaped escape VBN cord-022235-6ircruag 145 5 " " `` cord-022235-6ircruag 145 6 endogenous endogenous JJ cord-022235-6ircruag 145 7 ER ER NNP cord-022235-6ircruag 145 8 proteins protein NNS cord-022235-6ircruag 145 9 , , , cord-022235-6ircruag 145 10 rather rather RB cord-022235-6ircruag 145 11 than than IN cord-022235-6ircruag 145 12 receptormediated receptormediated JJ cord-022235-6ircruag 145 13 retention retention NN cord-022235-6ircruag 145 14 in in IN cord-022235-6ircruag 145 15 the the DT cord-022235-6ircruag 145 16 ER ER NNP cord-022235-6ircruag 145 17 , , , cord-022235-6ircruag 145 18 is be VBZ cord-022235-6ircruag 145 19 the the DT cord-022235-6ircruag 145 20 currently currently RB cord-022235-6ircruag 145 21 favored favor VBN cord-022235-6ircruag 145 22 model model NN cord-022235-6ircruag 145 23 for for IN cord-022235-6ircruag 145 24 the the DT cord-022235-6ircruag 145 25 specific specific JJ cord-022235-6ircruag 145 26 accumulation accumulation NN cord-022235-6ircruag 145 27 of of IN cord-022235-6ircruag 145 28 proteins protein NNS cord-022235-6ircruag 145 29 in in IN cord-022235-6ircruag 145 30 the the DT cord-022235-6ircruag 145 31 ER er NN cord-022235-6ircruag 145 32 ( ( -LRB- cord-022235-6ircruag 145 33 151 151 CD cord-022235-6ircruag 145 34 ) ) -RRB- cord-022235-6ircruag 145 35 . . . cord-022235-6ircruag 146 1 It -PRON- PRP cord-022235-6ircruag 146 2 is be VBZ cord-022235-6ircruag 146 3 not not RB cord-022235-6ircruag 146 4 clear clear JJ cord-022235-6ircruag 146 5 whether whether IN cord-022235-6ircruag 146 6 soluble soluble JJ cord-022235-6ircruag 146 7 and and CC cord-022235-6ircruag 146 8 ER ER NNP cord-022235-6ircruag 146 9 membrane membrane NN cord-022235-6ircruag 146 10 proteins protein NNS cord-022235-6ircruag 146 11 are be VBP cord-022235-6ircruag 146 12 both both DT cord-022235-6ircruag 146 13 subject subject JJ cord-022235-6ircruag 146 14 to to IN cord-022235-6ircruag 146 15 the the DT cord-022235-6ircruag 146 16 same same JJ cord-022235-6ircruag 146 17 retention retention NN cord-022235-6ircruag 146 18 mechanism mechanism NN cord-022235-6ircruag 146 19 , , , cord-022235-6ircruag 146 20 but but CC cord-022235-6ircruag 146 21 studies study NNS cord-022235-6ircruag 146 22 on on IN cord-022235-6ircruag 146 23 the the DT cord-022235-6ircruag 146 24 rates rate NNS cord-022235-6ircruag 146 25 of of IN cord-022235-6ircruag 146 26 diffusion diffusion NN cord-022235-6ircruag 146 27 of of IN cord-022235-6ircruag 146 28 membrane membrane NN cord-022235-6ircruag 146 29 proteins protein NNS cord-022235-6ircruag 146 30 in in IN cord-022235-6ircruag 146 31 the the DT cord-022235-6ircruag 146 32 ER ER NNP cord-022235-6ircruag 146 33 indicate indicate VBP cord-022235-6ircruag 146 34 that that IN cord-022235-6ircruag 146 35 RERM RERM NNP cord-022235-6ircruag 146 36 proteins protein NNS cord-022235-6ircruag 146 37 are be VBP cord-022235-6ircruag 146 38 more more RBR cord-022235-6ircruag 146 39 restricted restricted JJ cord-022235-6ircruag 146 40 than than IN cord-022235-6ircruag 146 41 BiP BiP NNP cord-022235-6ircruag 146 42 and and CC cord-022235-6ircruag 146 43 that that IN cord-022235-6ircruag 146 44 the the DT cord-022235-6ircruag 146 45 mobility mobility NN cord-022235-6ircruag 146 46 of of IN cord-022235-6ircruag 146 47 BiP BiP NNP cord-022235-6ircruag 146 48 devoid devoid JJ cord-022235-6ircruag 146 49 of of IN cord-022235-6ircruag 146 50 its -PRON- PRP$ cord-022235-6ircruag 146 51 ER ER NNP cord-022235-6ircruag 146 52 retention retention NN cord-022235-6ircruag 146 53 - - HYPH cord-022235-6ircruag 146 54 salvage salvage NN cord-022235-6ircruag 146 55 signal signal NN cord-022235-6ircruag 146 56 ( ( -LRB- cord-022235-6ircruag 146 57 see see VB cord-022235-6ircruag 146 58 below below RB cord-022235-6ircruag 146 59 ) ) -RRB- cord-022235-6ircruag 146 60 can can MD cord-022235-6ircruag 146 61 not not RB cord-022235-6ircruag 146 62 be be VB cord-022235-6ircruag 146 63 distinguished distinguish VBN cord-022235-6ircruag 146 64 from from IN cord-022235-6ircruag 146 65 that that DT cord-022235-6ircruag 146 66 of of IN cord-022235-6ircruag 146 67 normal normal JJ cord-022235-6ircruag 146 68 BiP bip NN cord-022235-6ircruag 146 69 ( ( -LRB- cord-022235-6ircruag 146 70 151 151 CD cord-022235-6ircruag 146 71 ) ) -RRB- cord-022235-6ircruag 146 72 . . . cord-022235-6ircruag 147 1 Several several JJ cord-022235-6ircruag 147 2 studies study NNS cord-022235-6ircruag 147 3 have have VBP cord-022235-6ircruag 147 4 sought seek VBN cord-022235-6ircruag 147 5 to to TO cord-022235-6ircruag 147 6 determine determine VB cord-022235-6ircruag 147 7 the the DT cord-022235-6ircruag 147 8 nature nature NN cord-022235-6ircruag 147 9 of of IN cord-022235-6ircruag 147 10 the the DT cord-022235-6ircruag 147 11 ER ER NNP cord-022235-6ircruag 147 12 reten reten NN cord-022235-6ircruag 147 13 tion tion NN cord-022235-6ircruag 147 14 - - HYPH cord-022235-6ircruag 147 15 salvage salvage NN cord-022235-6ircruag 147 16 signal(s signal(s NNP cord-022235-6ircruag 147 17 ) ) -RRB- cord-022235-6ircruag 147 18 . . . cord-022235-6ircruag 148 1 The the DT cord-022235-6ircruag 148 2 rotaviral rotaviral JJ cord-022235-6ircruag 148 3 type type NN cord-022235-6ircruag 148 4 II II NNP cord-022235-6ircruag 148 5 ER ER NNP cord-022235-6ircruag 148 6 membrane membrane NN cord-022235-6ircruag 148 7 protein protein NN cord-022235-6ircruag 148 8 VP7 VP7 NNP cord-022235-6ircruag 148 9 was be VBD cord-022235-6ircruag 148 10 found find VBN cord-022235-6ircruag 148 11 to to TO cord-022235-6ircruag 148 12 be be VB cord-022235-6ircruag 148 13 secreted secrete VBN cord-022235-6ircruag 148 14 when when WRB cord-022235-6ircruag 148 15 the the DT cord-022235-6ircruag 148 16 two two CD cord-022235-6ircruag 148 17 potential potential JJ cord-022235-6ircruag 148 18 N n CD cord-022235-6ircruag 148 19 - - HYPH cord-022235-6ircruag 148 20 terminal terminal JJ cord-022235-6ircruag 148 21 transmem transmem NN cord-022235-6ircruag 148 22 brane brane NN cord-022235-6ircruag 148 23 segments segment NNS cord-022235-6ircruag 148 24 were be VBD cord-022235-6ircruag 148 25 deleted delete VBN cord-022235-6ircruag 148 26 ( ( -LRB- cord-022235-6ircruag 148 27 884 884 CD cord-022235-6ircruag 148 28 ) ) -RRB- cord-022235-6ircruag 148 29 , , , cord-022235-6ircruag 148 30 suggesting suggest VBG cord-022235-6ircruag 148 31 that that IN cord-022235-6ircruag 148 32 retention retention NN cord-022235-6ircruag 148 33 - - HYPH cord-022235-6ircruag 148 34 salvage salvage NN cord-022235-6ircruag 148 35 de de FW cord-022235-6ircruag 148 36 pended pende VBN cord-022235-6ircruag 148 37 on on IN cord-022235-6ircruag 148 38 a a DT cord-022235-6ircruag 148 39 membrane membrane NN cord-022235-6ircruag 148 40 anchor anchor NN cord-022235-6ircruag 148 41 domain domain NN cord-022235-6ircruag 148 42 . . . cord-022235-6ircruag 149 1 However however RB cord-022235-6ircruag 149 2 , , , cord-022235-6ircruag 149 3 subsequent subsequent JJ cord-022235-6ircruag 149 4 studies study NNS cord-022235-6ircruag 149 5 showed show VBD cord-022235-6ircruag 149 6 that that IN cord-022235-6ircruag 149 7 the the DT cord-022235-6ircruag 149 8 entire entire JJ cord-022235-6ircruag 149 9 N n CD cord-022235-6ircruag 149 10 - - HYPH cord-022235-6ircruag 149 11 terminus terminus NN cord-022235-6ircruag 149 12 was be VBD cord-022235-6ircruag 149 13 absent absent JJ cord-022235-6ircruag 149 14 from from IN cord-022235-6ircruag 149 15 mature mature JJ cord-022235-6ircruag 149 16 VP7 vp7 NN cord-022235-6ircruag 149 17 ( ( -LRB- cord-022235-6ircruag 149 18 1084 1084 CD cord-022235-6ircruag 149 19 ) ) -RRB- cord-022235-6ircruag 149 20 and and CC cord-022235-6ircruag 149 21 thus thus RB cord-022235-6ircruag 149 22 that that IN cord-022235-6ircruag 149 23 some some DT cord-022235-6ircruag 149 24 other other JJ cord-022235-6ircruag 149 25 feature feature NN cord-022235-6ircruag 149 26 must must MD cord-022235-6ircruag 149 27 account account VB cord-022235-6ircruag 149 28 for for IN cord-022235-6ircruag 149 29 VP7 vp7 JJ cord-022235-6ircruag 149 30 retention retention NN cord-022235-6ircruag 149 31 in in IN cord-022235-6ircruag 149 32 the the DT cord-022235-6ircruag 149 33 ER er NN cord-022235-6ircruag 149 34 ( ( -LRB- cord-022235-6ircruag 149 35 883c 883c CD cord-022235-6ircruag 149 36 ) ) -RRB- cord-022235-6ircruag 149 37 . . . cord-022235-6ircruag 150 1 Although although IN cord-022235-6ircruag 150 2 deletion deletion NN cord-022235-6ircruag 150 3 of of IN cord-022235-6ircruag 150 4 the the DT cord-022235-6ircruag 150 5 C C NNP cord-022235-6ircruag 150 6 - - HYPH cord-022235-6ircruag 150 7 terminal terminal NN cord-022235-6ircruag 150 8 transmembrane transmembrane NN cord-022235-6ircruag 150 9 and and CC cord-022235-6ircruag 150 10 cyto cyto JJ cord-022235-6ircruag 150 11 plasmic plasmic JJ cord-022235-6ircruag 150 12 tails tail NNS cord-022235-6ircruag 150 13 of of IN cord-022235-6ircruag 150 14 the the DT cord-022235-6ircruag 150 15 type type NN cord-022235-6ircruag 150 16 I -PRON- PRP cord-022235-6ircruag 150 17 ER ER NNP cord-022235-6ircruag 150 18 membrane membrane NN cord-022235-6ircruag 150 19 protein protein NN cord-022235-6ircruag 150 20 Ε19 Ε19 NNP cord-022235-6ircruag 150 21 of of IN cord-022235-6ircruag 150 22 adenovirus adenovirus NN cord-022235-6ircruag 150 23 also also RB cord-022235-6ircruag 150 24 causes cause VBZ cord-022235-6ircruag 150 25 the the DT cord-022235-6ircruag 150 26 protein protein NN cord-022235-6ircruag 150 27 to to TO cord-022235-6ircruag 150 28 be be VB cord-022235-6ircruag 150 29 secreted secrete VBN cord-022235-6ircruag 150 30 , , , cord-022235-6ircruag 150 31 deletion deletion NN cord-022235-6ircruag 150 32 of of IN cord-022235-6ircruag 150 33 the the DT cord-022235-6ircruag 150 34 last last JJ cord-022235-6ircruag 150 35 eight eight CD cord-022235-6ircruag 150 36 residues residue NNS cord-022235-6ircruag 150 37 ( ( -LRB- cord-022235-6ircruag 150 38 FIDEKKMP FIDEKKMP NNP cord-022235-6ircruag 150 39 ) ) -RRB- cord-022235-6ircruag 150 40 of of IN cord-022235-6ircruag 150 41 the the DT cord-022235-6ircruag 150 42 C C NNP cord-022235-6ircruag 150 43 - - HYPH cord-022235-6ircruag 150 44 terminal terminal NN cord-022235-6ircruag 150 45 , , , cord-022235-6ircruag 150 46 cytoplasmic cytoplasmic JJ cord-022235-6ircruag 150 47 tail tail NN cord-022235-6ircruag 150 48 alone alone RB cord-022235-6ircruag 150 49 causes cause VBZ cord-022235-6ircruag 150 50 the the DT cord-022235-6ircruag 150 51 protein protein NN cord-022235-6ircruag 150 52 to to TO cord-022235-6ircruag 150 53 appear appear VB cord-022235-6ircruag 150 54 on on IN cord-022235-6ircruag 150 55 the the DT cord-022235-6ircruag 150 56 cell cell NN cord-022235-6ircruag 150 57 surface surface NN cord-022235-6ircruag 150 58 , , , cord-022235-6ircruag 150 59 suggesting suggest VBG cord-022235-6ircruag 150 60 that that IN cord-022235-6ircruag 150 61 the the DT cord-022235-6ircruag 150 62 C C NNP cord-022235-6ircruag 150 63 - - HYPH cord-022235-6ircruag 150 64 terminus terminus NN cord-022235-6ircruag 150 65 contains contain VBZ cord-022235-6ircruag 150 66 the the DT cord-022235-6ircruag 150 67 ER ER NNP cord-022235-6ircruag 150 68 retention retention NN cord-022235-6ircruag 150 69 - - HYPH cord-022235-6ircruag 150 70 salvage salvage NN cord-022235-6ircruag 150 71 signal signal NN cord-022235-6ircruag 150 72 . . . cord-022235-6ircruag 151 1 Furthermore furthermore RB cord-022235-6ircruag 151 2 , , , cord-022235-6ircruag 151 3 cell cell NN cord-022235-6ircruag 151 4 - - HYPH cord-022235-6ircruag 151 5 surface surface NN cord-022235-6ircruag 151 6 interleukin interleukin NN cord-022235-6ircruag 151 7 2 2 CD cord-022235-6ircruag 151 8 re re NN cord-022235-6ircruag 151 9 ceptor ceptor NNP cord-022235-6ircruag 151 10 protein protein NNP cord-022235-6ircruag 151 11 β β NNP cord-022235-6ircruag 151 12 chain chain NN cord-022235-6ircruag 151 13 was be VBD cord-022235-6ircruag 151 14 converted convert VBN cord-022235-6ircruag 151 15 into into IN cord-022235-6ircruag 151 16 a a DT cord-022235-6ircruag 151 17 resident resident JJ cord-022235-6ircruag 151 18 ER ER NNP cord-022235-6ircruag 151 19 protein protein NN cord-022235-6ircruag 151 20 following follow VBG cord-022235-6ircruag 151 21 fusion fusion NN cord-022235-6ircruag 151 22 of of IN cord-022235-6ircruag 151 23 FIDEKKMP FIDEKKMP NNP cord-022235-6ircruag 151 24 to to IN cord-022235-6ircruag 151 25 its -PRON- PRP$ cord-022235-6ircruag 151 26 cytoplasmic cytoplasmic JJ cord-022235-6ircruag 151 27 C c NN cord-022235-6ircruag 151 28 - - HYPH cord-022235-6ircruag 151 29 terminus terminus NN cord-022235-6ircruag 151 30 ( ( -LRB- cord-022235-6ircruag 151 31 832 832 CD cord-022235-6ircruag 151 32 ) ) -RRB- cord-022235-6ircruag 151 33 . . . cord-022235-6ircruag 152 1 The the DT cord-022235-6ircruag 152 2 cytoplas- cytoplas- JJ cord-022235-6ircruag 152 3 mically mically RB cord-022235-6ircruag 152 4 exposed expose VBD cord-022235-6ircruag 152 5 signal signal NN cord-022235-6ircruag 152 6 could could MD cord-022235-6ircruag 152 7 be be VB cord-022235-6ircruag 152 8 recognized recognize VBN cord-022235-6ircruag 152 9 by by IN cord-022235-6ircruag 152 10 " " `` cord-022235-6ircruag 152 11 salvage salvage NN cord-022235-6ircruag 152 12 receptors receptor NNS cord-022235-6ircruag 152 13 " " '' cord-022235-6ircruag 152 14 in in IN cord-022235-6ircruag 152 15 the the DT cord-022235-6ircruag 152 16 transitional transitional JJ cord-022235-6ircruag 152 17 element element NN cord-022235-6ircruag 152 18 . . . cord-022235-6ircruag 153 1 These these DT cord-022235-6ircruag 153 2 studies study NNS cord-022235-6ircruag 153 3 on on IN cord-022235-6ircruag 153 4 the the DT cord-022235-6ircruag 153 5 Ε19 Ε19 NNP cord-022235-6ircruag 153 6 protein protein NN cord-022235-6ircruag 153 7 are be VBP cord-022235-6ircruag 153 8 reminiscent reminiscent JJ cord-022235-6ircruag 153 9 of of IN cord-022235-6ircruag 153 10 earlier early JJR cord-022235-6ircruag 153 11 , , , cord-022235-6ircruag 153 12 seminal seminal JJ cord-022235-6ircruag 153 13 work work NN cord-022235-6ircruag 153 14 on on IN cord-022235-6ircruag 153 15 the the DT cord-022235-6ircruag 153 16 soluble soluble JJ cord-022235-6ircruag 153 17 ER ER NNP cord-022235-6ircruag 153 18 protein protein NN cord-022235-6ircruag 153 19 BiP bip NN cord-022235-6ircruag 153 20 which which WDT cord-022235-6ircruag 153 21 resulted result VBD cord-022235-6ircruag 153 22 from from IN cord-022235-6ircruag 153 23 the the DT cord-022235-6ircruag 153 24 observation observation NN cord-022235-6ircruag 153 25 that that IN cord-022235-6ircruag 153 26 three three CD cord-022235-6ircruag 153 27 soluble soluble JJ cord-022235-6ircruag 153 28 proteins protein NNS cord-022235-6ircruag 153 29 in in IN cord-022235-6ircruag 153 30 the the DT cord-022235-6ircruag 153 31 lumen lumen NN cord-022235-6ircruag 153 32 of of IN cord-022235-6ircruag 153 33 the the DT cord-022235-6ircruag 153 34 ER ER NNP cord-022235-6ircruag 153 35 , , , cord-022235-6ircruag 153 36 including include VBG cord-022235-6ircruag 153 37 BiP BiP NNP cord-022235-6ircruag 153 38 , , , cord-022235-6ircruag 153 39 had have VBD cord-022235-6ircruag 153 40 the the DT cord-022235-6ircruag 153 41 same same JJ cord-022235-6ircruag 153 42 four four CD cord-022235-6ircruag 153 43 residues residue NNS cord-022235-6ircruag 153 44 , , , cord-022235-6ircruag 153 45 Lys Lys NNP cord-022235-6ircruag 153 46 - - HYPH cord-022235-6ircruag 153 47 Asp Asp NNP cord-022235-6ircruag 153 48 - - HYPH cord-022235-6ircruag 153 49 Glu Glu NNP cord-022235-6ircruag 153 50 - - HYPH cord-022235-6ircruag 153 51 Leu Leu NNP cord-022235-6ircruag 153 52 ( ( -LRB- cord-022235-6ircruag 153 53 KDEL KDEL NNP cord-022235-6ircruag 153 54 ) ) -RRB- cord-022235-6ircruag 153 55 , , , cord-022235-6ircruag 153 56 at at IN cord-022235-6ircruag 153 57 their -PRON- PRP$ cord-022235-6ircruag 153 58 C c NN cord-022235-6ircruag 153 59 - - HYPH cord-022235-6ircruag 153 60 termini termini NNP cord-022235-6ircruag 153 61 . . . cord-022235-6ircruag 154 1 Munro Munro NNP cord-022235-6ircruag 154 2 and and CC cord-022235-6ircruag 154 3 Pelham Pelham NNP cord-022235-6ircruag 154 4 ( ( -LRB- cord-022235-6ircruag 154 5 759 759 CD cord-022235-6ircruag 154 6 ) ) -RRB- cord-022235-6ircruag 154 7 found find VBD cord-022235-6ircruag 154 8 that that IN cord-022235-6ircruag 154 9 if if IN cord-022235-6ircruag 154 10 this this DT cord-022235-6ircruag 154 11 sequence sequence NN cord-022235-6ircruag 154 12 was be VBD cord-022235-6ircruag 154 13 deleted delete VBN cord-022235-6ircruag 154 14 or or CC cord-022235-6ircruag 154 15 extended extend VBN cord-022235-6ircruag 154 16 , , , cord-022235-6ircruag 154 17 BiP BiP NNP cord-022235-6ircruag 154 18 was be VBD cord-022235-6ircruag 154 19 secreted secrete VBN cord-022235-6ircruag 154 20 into into IN cord-022235-6ircruag 154 21 the the DT cord-022235-6ircruag 154 22 medium medium NN cord-022235-6ircruag 154 23 . . . cord-022235-6ircruag 155 1 This this DT cord-022235-6ircruag 155 2 suggests suggest VBZ cord-022235-6ircruag 155 3 that that IN cord-022235-6ircruag 155 4 KDEL KDEL NNP cord-022235-6ircruag 155 5 is be VBZ cord-022235-6ircruag 155 6 the the DT cord-022235-6ircruag 155 7 ER ER NNP cord-022235-6ircruag 155 8 reten reten NN cord-022235-6ircruag 155 9 - - HYPH cord-022235-6ircruag 155 10 tion tion NN cord-022235-6ircruag 155 11 signal signal NN cord-022235-6ircruag 155 12 and and CC cord-022235-6ircruag 155 13 that that IN cord-022235-6ircruag 155 14 it -PRON- PRP cord-022235-6ircruag 155 15 must must MD cord-022235-6ircruag 155 16 be be VB cord-022235-6ircruag 155 17 at at IN cord-022235-6ircruag 155 18 the the DT cord-022235-6ircruag 155 19 extreme extreme JJ cord-022235-6ircruag 155 20 C c NN cord-022235-6ircruag 155 21 - - HYPH cord-022235-6ircruag 155 22 terminus terminus NN cord-022235-6ircruag 155 23 , , , cord-022235-6ircruag 155 24 which which WDT cord-022235-6ircruag 155 25 is be VBZ cord-022235-6ircruag 155 26 pre pre JJ cord-022235-6ircruag 155 27 sumably sumably RB cord-022235-6ircruag 156 1 the the DT cord-022235-6ircruag 156 2 last last JJ cord-022235-6ircruag 156 3 segment segment NN cord-022235-6ircruag 156 4 of of IN cord-022235-6ircruag 156 5 the the DT cord-022235-6ircruag 156 6 polypeptide polypeptide NN cord-022235-6ircruag 156 7 to to TO cord-022235-6ircruag 156 8 fold fold VB cord-022235-6ircruag 157 1 and and CC cord-022235-6ircruag 157 2 may may MD cord-022235-6ircruag 157 3 therefore therefore RB cord-022235-6ircruag 157 4 be be VB cord-022235-6ircruag 157 5 exposed expose VBN cord-022235-6ircruag 157 6 on on IN cord-022235-6ircruag 157 7 the the DT cord-022235-6ircruag 157 8 surface surface NN cord-022235-6ircruag 157 9 of of IN cord-022235-6ircruag 157 10 the the DT cord-022235-6ircruag 157 11 protein protein NN cord-022235-6ircruag 157 12 . . . cord-022235-6ircruag 158 1 In in IN cord-022235-6ircruag 158 2 a a DT cord-022235-6ircruag 158 3 complementary complementary JJ cord-022235-6ircruag 158 4 study study NN cord-022235-6ircruag 158 5 , , , cord-022235-6ircruag 158 6 DNA dna NN cord-022235-6ircruag 158 7 coding coding NN cord-022235-6ircruag 158 8 for for IN cord-022235-6ircruag 158 9 KDEL KDEL NNP cord-022235-6ircruag 158 10 was be VBD cord-022235-6ircruag 158 11 fused fuse VBN cord-022235-6ircruag 158 12 to to IN cord-022235-6ircruag 158 13 the the DT cord-022235-6ircruag 158 14 end end NN cord-022235-6ircruag 158 15 of of IN cord-022235-6ircruag 158 16 a a DT cord-022235-6ircruag 158 17 cDNA cdna NN cord-022235-6ircruag 158 18 clone clone NN cord-022235-6ircruag 158 19 coding coding NN cord-022235-6ircruag 158 20 for for IN cord-022235-6ircruag 158 21 secreted secrete VBN cord-022235-6ircruag 158 22 lysozyme lysozyme NNS cord-022235-6ircruag 158 23 . . . cord-022235-6ircruag 159 1 The the DT cord-022235-6ircruag 159 2 resulting result VBG cord-022235-6ircruag 159 3 hybrid hybrid JJ cord-022235-6ircruag 159 4 protein protein NN cord-022235-6ircruag 159 5 was be VBD cord-022235-6ircruag 159 6 retained retain VBN cord-022235-6ircruag 159 7 in in IN cord-022235-6ircruag 159 8 a a DT cord-022235-6ircruag 159 9 perinu perinu JJ cord-022235-6ircruag 159 10 clear clear JJ cord-022235-6ircruag 159 11 region region NN cord-022235-6ircruag 159 12 probably probably RB cord-022235-6ircruag 159 13 corresponding correspond VBG cord-022235-6ircruag 159 14 to to IN cord-022235-6ircruag 159 15 the the DT cord-022235-6ircruag 159 16 ER ER NNP cord-022235-6ircruag 159 17 ( ( -LRB- cord-022235-6ircruag 159 18 759 759 CD cord-022235-6ircruag 159 19 ) ) -RRB- cord-022235-6ircruag 159 20 . . . cord-022235-6ircruag 160 1 Further further JJ cord-022235-6ircruag 160 2 studies study NNS cord-022235-6ircruag 160 3 are be VBP cord-022235-6ircruag 160 4 required require VBN cord-022235-6ircruag 160 5 to to TO cord-022235-6ircruag 160 6 determine determine VB cord-022235-6ircruag 160 7 whether whether IN cord-022235-6ircruag 160 8 KDEL KDEL NNP cord-022235-6ircruag 160 9 is be VBZ cord-022235-6ircruag 160 10 the the DT cord-022235-6ircruag 160 11 " " `` cord-022235-6ircruag 160 12 uni uni NNP cord-022235-6ircruag 160 13 versal versal FW cord-022235-6ircruag 160 14 " " '' cord-022235-6ircruag 160 15 ER ER NNP cord-022235-6ircruag 160 16 retention retention NN cord-022235-6ircruag 160 17 signal signal NN cord-022235-6ircruag 160 18 . . . cord-022235-6ircruag 161 1 Preliminary preliminary JJ cord-022235-6ircruag 161 2 studies study NNS cord-022235-6ircruag 161 3 suggest suggest VBP cord-022235-6ircruag 161 4 that that IN cord-022235-6ircruag 161 5 some some DT cord-022235-6ircruag 161 6 mam mam NN cord-022235-6ircruag 161 7 malian malian JJ cord-022235-6ircruag 161 8 ER ER NNP cord-022235-6ircruag 161 9 proteins protein NNS cord-022235-6ircruag 161 10 have have VBP cord-022235-6ircruag 161 11 the the DT cord-022235-6ircruag 161 12 sequence sequence NN cord-022235-6ircruag 161 13 RDEL RDEL NNP cord-022235-6ircruag 161 14 at at IN cord-022235-6ircruag 161 15 the the DT cord-022235-6ircruag 161 16 C c NN cord-022235-6ircruag 161 17 - - HYPH cord-022235-6ircruag 161 18 terminus terminus NN cord-022235-6ircruag 161 19 , , , cord-022235-6ircruag 161 20 whereas whereas IN cord-022235-6ircruag 161 21 yeast yeast NN cord-022235-6ircruag 161 22 ER er NN cord-022235-6ircruag 161 23 proteins protein NNS cord-022235-6ircruag 161 24 have have VBP cord-022235-6ircruag 161 25 the the DT cord-022235-6ircruag 161 26 sequence sequence NN cord-022235-6ircruag 161 27 HDEL HDEL NNP cord-022235-6ircruag 161 28 ( ( -LRB- cord-022235-6ircruag 161 29 858a 858a NNP cord-022235-6ircruag 161 30 ) ) -RRB- cord-022235-6ircruag 161 31 . . . cord-022235-6ircruag 162 1 HDEL HDEL NNP cord-022235-6ircruag 162 2 or or CC cord-022235-6ircruag 162 3 KDEL KDEL NNP cord-022235-6ircruag 162 4 is be VBZ cord-022235-6ircruag 162 5 presumably presumably RB cord-022235-6ircruag 162 6 recognized recognize VBN cord-022235-6ircruag 162 7 either either CC cord-022235-6ircruag 162 8 by by IN cord-022235-6ircruag 162 9 an an DT cord-022235-6ircruag 162 10 endogenous endogenous JJ cord-022235-6ircruag 162 11 ER ER NNP cord-022235-6ircruag 162 12 membrane membrane NN cord-022235-6ircruag 162 13 protein protein NN cord-022235-6ircruag 162 14 , , , cord-022235-6ircruag 162 15 which which WDT cord-022235-6ircruag 162 16 could could MD cord-022235-6ircruag 162 17 anchor anchor VB cord-022235-6ircruag 162 18 soluble soluble JJ cord-022235-6ircruag 162 19 proteins protein NNS cord-022235-6ircruag 162 20 in in IN cord-022235-6ircruag 162 21 the the DT cord-022235-6ircruag 162 22 ER er NN cord-022235-6ircruag 162 23 , , , cord-022235-6ircruag 162 24 or or CC cord-022235-6ircruag 162 25 , , , cord-022235-6ircruag 162 26 more more RBR cord-022235-6ircruag 162 27 likely likely JJ cord-022235-6ircruag 162 28 , , , cord-022235-6ircruag 162 29 by by IN cord-022235-6ircruag 162 30 the the DT cord-022235-6ircruag 162 31 recycling recycling NN cord-022235-6ircruag 162 32 receptor receptor NN cord-022235-6ircruag 162 33 located locate VBN cord-022235-6ircruag 162 34 in in IN cord-022235-6ircruag 162 35 the the DT cord-022235-6ircruag 162 36 transition transition NN cord-022235-6ircruag 162 37 element element NN cord-022235-6ircruag 162 38 or or CC cord-022235-6ircruag 162 39 cis cis NNP cord-022235-6ircruag 162 40 Golgi Golgi NNP cord-022235-6ircruag 162 41 . . . cord-022235-6ircruag 163 1 Indeed indeed RB cord-022235-6ircruag 163 2 , , , cord-022235-6ircruag 163 3 a a DT cord-022235-6ircruag 163 4 putative putative JJ cord-022235-6ircruag 163 5 HDEL HDEL NNP cord-022235-6ircruag 163 6 receptor receptor NN cord-022235-6ircruag 163 7 gene gene NN cord-022235-6ircruag 163 8 ( ( -LRB- cord-022235-6ircruag 163 9 ERD1 ERD1 NNP cord-022235-6ircruag 163 10 ) ) -RRB- cord-022235-6ircruag 163 11 has have VBZ cord-022235-6ircruag 163 12 recently recently RB cord-022235-6ircruag 163 13 been be VBN cord-022235-6ircruag 163 14 identified identify VBN cord-022235-6ircruag 163 15 in in IN cord-022235-6ircruag 163 16 yeast yeast NN cord-022235-6ircruag 163 17 cells cell NNS cord-022235-6ircruag 163 18 ( ( -LRB- cord-022235-6ircruag 163 19 858,858a 858,858a CD cord-022235-6ircruag 163 20 ) ) -RRB- cord-022235-6ircruag 163 21 . . . cord-022235-6ircruag 164 1 It -PRON- PRP cord-022235-6ircruag 164 2 is be VBZ cord-022235-6ircruag 164 3 not not RB cord-022235-6ircruag 164 4 clear clear JJ cord-022235-6ircruag 164 5 what what WP cord-022235-6ircruag 164 6 triggers trigger VBZ cord-022235-6ircruag 164 7 the the DT cord-022235-6ircruag 164 8 release release NN cord-022235-6ircruag 164 9 of of IN cord-022235-6ircruag 164 10 BiP BiP NNP cord-022235-6ircruag 164 11 from from IN cord-022235-6ircruag 164 12 its -PRON- PRP$ cord-022235-6ircruag 164 13 receptor receptor NN cord-022235-6ircruag 164 14 once once IN cord-022235-6ircruag 164 15 it -PRON- PRP cord-022235-6ircruag 164 16 is be VBZ cord-022235-6ircruag 164 17 recycled recycle VBN cord-022235-6ircruag 164 18 to to IN cord-022235-6ircruag 164 19 the the DT cord-022235-6ircruag 164 20 ER ER NNP cord-022235-6ircruag 164 21 . . . cord-022235-6ircruag 165 1 β β LS cord-022235-6ircruag 165 2 - - HYPH cord-022235-6ircruag 165 3 Glucanase Glucanase NNP cord-022235-6ircruag 165 4 is be VBZ cord-022235-6ircruag 165 5 a a DT cord-022235-6ircruag 165 6 typical typical JJ cord-022235-6ircruag 165 7 lysosomal lysosomal JJ cord-022235-6ircruag 165 8 protein protein NN cord-022235-6ircruag 165 9 ; ; : cord-022235-6ircruag 165 10 small small JJ cord-022235-6ircruag 165 11 but but CC cord-022235-6ircruag 165 12 significant significant JJ cord-022235-6ircruag 165 13 amounts amount NNS cord-022235-6ircruag 165 14 of of IN cord-022235-6ircruag 165 15 it -PRON- PRP cord-022235-6ircruag 165 16 , , , cord-022235-6ircruag 165 17 however however RB cord-022235-6ircruag 165 18 , , , cord-022235-6ircruag 165 19 are be VBP cord-022235-6ircruag 165 20 retained retain VBN cord-022235-6ircruag 165 21 in in IN cord-022235-6ircruag 165 22 the the DT cord-022235-6ircruag 165 23 ER ER NNP cord-022235-6ircruag 165 24 through through IN cord-022235-6ircruag 165 25 a a DT cord-022235-6ircruag 165 26 specific specific JJ cord-022235-6ircruag 165 27 interac interac NN cord-022235-6ircruag 165 28 tion tion NN cord-022235-6ircruag 165 29 with with IN cord-022235-6ircruag 165 30 an an DT cord-022235-6ircruag 165 31 endogeneous endogeneous JJ cord-022235-6ircruag 165 32 ER ER NNP cord-022235-6ircruag 165 33 protein protein NN cord-022235-6ircruag 165 34 , , , cord-022235-6ircruag 165 35 the the DT cord-022235-6ircruag 165 36 esterase esterase NN cord-022235-6ircruag 165 37 egasyn egasyn NN cord-022235-6ircruag 165 38 . . . cord-022235-6ircruag 166 1 Studies study NNS cord-022235-6ircruag 166 2 by by IN cord-022235-6ircruag 166 3 Medda Medda NNP cord-022235-6ircruag 166 4 et et NNP cord-022235-6ircruag 166 5 al al NNP cord-022235-6ircruag 166 6 . . . cord-022235-6ircruag 167 1 ( ( -LRB- cord-022235-6ircruag 167 2 704 704 LS cord-022235-6ircruag 167 3 ) ) -RRB- cord-022235-6ircruag 167 4 indicate indicate VBP cord-022235-6ircruag 167 5 that that IN cord-022235-6ircruag 167 6 /3-glucanase /3-glucanase . cord-022235-6ircruag 167 7 - - HYPH cord-022235-6ircruag 167 8 egasyn egasyn NN cord-022235-6ircruag 167 9 interaction interaction NN cord-022235-6ircruag 167 10 is be VBZ cord-022235-6ircruag 167 11 blocked block VBN cord-022235-6ircruag 167 12 by by IN cord-022235-6ircruag 167 13 inhibitors inhibitor NNS cord-022235-6ircruag 167 14 of of IN cord-022235-6ircruag 167 15 esterase esterase NN cord-022235-6ircruag 167 16 activity activity NN cord-022235-6ircruag 167 17 , , , cord-022235-6ircruag 167 18 leading lead VBG cord-022235-6ircruag 167 19 to to IN cord-022235-6ircruag 167 20 β β NN cord-022235-6ircruag 167 21 - - HYPH cord-022235-6ircruag 167 22 glucanase glucanase JJ cord-022235-6ircruag 167 23 secretion secretion NN cord-022235-6ircruag 167 24 ( ( -LRB- cord-022235-6ircruag 167 25 rather rather RB cord-022235-6ircruag 167 26 than than IN cord-022235-6ircruag 167 27 sorting sort VBG cord-022235-6ircruag 167 28 to to IN cord-022235-6ircruag 167 29 the the DT cord-022235-6ircruag 167 30 lysosome lysosome NN cord-022235-6ircruag 167 31 ) ) -RRB- cord-022235-6ircruag 167 32 . . . cord-022235-6ircruag 168 1 Whether whether IN cord-022235-6ircruag 168 2 this this DT cord-022235-6ircruag 168 3 is be VBZ cord-022235-6ircruag 168 4 a a DT cord-022235-6ircruag 168 5 physiologically physiologically RB cord-022235-6ircruag 168 6 significant significant JJ cord-022235-6ircruag 168 7 mechanism mechanism NN cord-022235-6ircruag 168 8 for for IN cord-022235-6ircruag 168 9 retaining retain VBG cord-022235-6ircruag 168 10 proteins protein NNS cord-022235-6ircruag 168 11 in in IN cord-022235-6ircruag 168 12 the the DT cord-022235-6ircruag 168 13 ER ER NNP cord-022235-6ircruag 168 14 remains remain VBZ cord-022235-6ircruag 168 15 to to TO cord-022235-6ircruag 168 16 be be VB cord-022235-6ircruag 168 17 de de FW cord-022235-6ircruag 168 18 termined termined FW cord-022235-6ircruag 168 19 . . . cord-022235-6ircruag 169 1 Only only RB cord-022235-6ircruag 169 2 about about IN cord-022235-6ircruag 169 3 10 10 CD cord-022235-6ircruag 169 4 % % NN cord-022235-6ircruag 169 5 of of IN cord-022235-6ircruag 169 6 egasyn egasyn NN cord-022235-6ircruag 169 7 is be VBZ cord-022235-6ircruag 169 8 complexed complexe VBN cord-022235-6ircruag 169 9 with with IN cord-022235-6ircruag 169 10 /3-glucanase /3-glucanase . cord-022235-6ircruag 169 11 ( ( -LRB- cord-022235-6ircruag 169 12 666 666 CD cord-022235-6ircruag 169 13 ) ) -RRB- cord-022235-6ircruag 169 14 , , , cord-022235-6ircruag 169 15 so so RB cord-022235-6ircruag 169 16 perhaps perhaps RB cord-022235-6ircruag 169 17 it -PRON- PRP cord-022235-6ircruag 169 18 also also RB cord-022235-6ircruag 169 19 binds bind VBZ cord-022235-6ircruag 169 20 to to IN cord-022235-6ircruag 169 21 other other JJ cord-022235-6ircruag 169 22 resident resident NN cord-022235-6ircruag 169 23 ER ER NNP cord-022235-6ircruag 169 24 proteins protein NNS cord-022235-6ircruag 169 25 . . . cord-022235-6ircruag 170 1 It -PRON- PRP cord-022235-6ircruag 170 2 would would MD cord-022235-6ircruag 170 3 be be VB cord-022235-6ircruag 170 4 inter inter NNP cord-022235-6ircruag 170 5 esting esting NN cord-022235-6ircruag 170 6 to to TO cord-022235-6ircruag 170 7 see see VB cord-022235-6ircruag 170 8 whether whether IN cord-022235-6ircruag 170 9 egasyn egasyn NN cord-022235-6ircruag 170 10 itself -PRON- PRP cord-022235-6ircruag 170 11 has have VBZ cord-022235-6ircruag 170 12 a a DT cord-022235-6ircruag 170 13 C c NN cord-022235-6ircruag 170 14 - - HYPH cord-022235-6ircruag 170 15 terminal terminal NN cord-022235-6ircruag 170 16 KDEL KDEL NNP cord-022235-6ircruag 170 17 - - HYPH cord-022235-6ircruag 170 18 like like JJ cord-022235-6ircruag 170 19 sequence sequence NN cord-022235-6ircruag 170 20 . . . cord-022235-6ircruag 171 1 As as IN cord-022235-6ircruag 171 2 discussed discuss VBN cord-022235-6ircruag 171 3 above above RB cord-022235-6ircruag 171 4 , , , cord-022235-6ircruag 171 5 secretory secretory JJ cord-022235-6ircruag 171 6 proteins protein NNS cord-022235-6ircruag 171 7 do do VBP cord-022235-6ircruag 171 8 not not RB cord-022235-6ircruag 171 9 move move VB cord-022235-6ircruag 171 10 to to IN cord-022235-6ircruag 171 11 the the DT cord-022235-6ircruag 171 12 cis cis NNP cord-022235-6ircruag 171 13 Golgi Golgi NNP cord-022235-6ircruag 171 14 until until IN cord-022235-6ircruag 171 15 they -PRON- PRP cord-022235-6ircruag 171 16 attain attain VBP cord-022235-6ircruag 171 17 an an DT cord-022235-6ircruag 171 18 exit exit NN cord-022235-6ircruag 171 19 - - HYPH cord-022235-6ircruag 171 20 competent competent JJ cord-022235-6ircruag 171 21 state state NN cord-022235-6ircruag 171 22 , , , cord-022235-6ircruag 171 23 and and CC cord-022235-6ircruag 171 24 some some DT cord-022235-6ircruag 171 25 proteins protein NNS cord-022235-6ircruag 171 26 are be VBP cord-022235-6ircruag 171 27 specifically specifically RB cord-022235-6ircruag 171 28 retained retain VBN cord-022235-6ircruag 171 29 in in IN cord-022235-6ircruag 171 30 the the DT cord-022235-6ircruag 171 31 ER er NN cord-022235-6ircruag 171 32 , , , cord-022235-6ircruag 171 33 probably probably RB cord-022235-6ircruag 171 34 by by IN cord-022235-6ircruag 171 35 recycling recycling NN cord-022235-6ircruag 171 36 of of IN cord-022235-6ircruag 171 37 escaped escape VBN cord-022235-6ircruag 171 38 proteins protein NNS cord-022235-6ircruag 171 39 . . . cord-022235-6ircruag 172 1 Both both DT cord-022235-6ircruag 172 2 nor nor CC cord-022235-6ircruag 172 3 mal mal JJ cord-022235-6ircruag 172 4 secretory secretory JJ cord-022235-6ircruag 172 5 proteins protein NNS cord-022235-6ircruag 172 6 ( ( -LRB- cord-022235-6ircruag 172 7 1223 1223 CD cord-022235-6ircruag 172 8 ) ) -RRB- cord-022235-6ircruag 172 9 and and CC cord-022235-6ircruag 172 10 exit exit NN cord-022235-6ircruag 172 11 - - HYPH cord-022235-6ircruag 172 12 incompetent incompetent JJ cord-022235-6ircruag 172 13 proteins protein NNS cord-022235-6ircruag 172 14 have have VBP cord-022235-6ircruag 172 15 been be VBN cord-022235-6ircruag 172 16 reported report VBN cord-022235-6ircruag 172 17 to to TO cord-022235-6ircruag 172 18 accumulate accumulate VB cord-022235-6ircruag 172 19 in in IN cord-022235-6ircruag 172 20 a a DT cord-022235-6ircruag 172 21 specific specific JJ cord-022235-6ircruag 172 22 region region NN cord-022235-6ircruag 172 23 of of IN cord-022235-6ircruag 172 24 the the DT cord-022235-6ircruag 172 25 smooth smooth JJ cord-022235-6ircruag 172 26 ER ER NNP cord-022235-6ircruag 172 27 , , , cord-022235-6ircruag 172 28 the the DT cord-022235-6ircruag 172 29 vacuolar vacuolar JJ cord-022235-6ircruag 172 30 transitional transitional JJ cord-022235-6ircruag 172 31 element element NN cord-022235-6ircruag 172 32 ( ( -LRB- cord-022235-6ircruag 172 33 79,996 79,996 CD cord-022235-6ircruag 172 34 ) ) -RRB- cord-022235-6ircruag 172 35 , , , cord-022235-6ircruag 172 36 from from IN cord-022235-6ircruag 172 37 which which WDT cord-022235-6ircruag 172 38 vesicles vesicle NNS cord-022235-6ircruag 172 39 either either CC cord-022235-6ircruag 172 40 migrate migrate VBP cord-022235-6ircruag 172 41 to to IN cord-022235-6ircruag 172 42 and and CC cord-022235-6ircruag 172 43 fuse fuse VB cord-022235-6ircruag 172 44 with with IN cord-022235-6ircruag 172 45 the the DT cord-022235-6ircruag 172 46 cis cis NNP cord-022235-6ircruag 172 47 Golgi Golgi NNP cord-022235-6ircruag 172 48 ( ( -LRB- cord-022235-6ircruag 172 49 41 41 CD cord-022235-6ircruag 172 50 ) ) -RRB- cord-022235-6ircruag 172 51 or or CC cord-022235-6ircruag 172 52 coalesce coalesce VB cord-022235-6ircruag 172 53 to to TO cord-022235-6ircruag 172 54 form form VB cord-022235-6ircruag 172 55 new new JJ cord-022235-6ircruag 172 56 Golgi Golgi NNP cord-022235-6ircruag 172 57 cisternae cisternae NN cord-022235-6ircruag 172 58 ( ( -LRB- cord-022235-6ircruag 172 59 797 797 CD cord-022235-6ircruag 172 60 ) ) -RRB- cord-022235-6ircruag 172 61 . . . cord-022235-6ircruag 173 1 Specific specific JJ cord-022235-6ircruag 173 2 membrane membrane NN cord-022235-6ircruag 173 3 proteins protein NNS cord-022235-6ircruag 173 4 [ [ -LRB- cord-022235-6ircruag 174 1 e.g. e.g. RB cord-022235-6ircruag 174 2 , , , cord-022235-6ircruag 174 3 the the DT cord-022235-6ircruag 174 4 product product NN cord-022235-6ircruag 174 5 of of IN cord-022235-6ircruag 174 6 the the DT cord-022235-6ircruag 174 7 SEC SEC NNP cord-022235-6ircruag 174 8 12 12 CD cord-022235-6ircruag 174 9 gene gene NN cord-022235-6ircruag 174 10 in in IN cord-022235-6ircruag 174 11 yeasts yeast NNS cord-022235-6ircruag 174 12 ( ( -LRB- cord-022235-6ircruag 174 13 767a 767a CD cord-022235-6ircruag 174 14 ) ) -RRB- cord-022235-6ircruag 174 15 ] ] -RRB- cord-022235-6ircruag 174 16 may may MD cord-022235-6ircruag 174 17 be be VB cord-022235-6ircruag 174 18 required require VBN cord-022235-6ircruag 174 19 to to TO cord-022235-6ircruag 174 20 form form VB cord-022235-6ircruag 174 21 this this DT cord-022235-6ircruag 174 22 specialized specialized JJ cord-022235-6ircruag 174 23 domain domain NN cord-022235-6ircruag 174 24 of of IN cord-022235-6ircruag 174 25 the the DT cord-022235-6ircruag 174 26 ER ER NNP cord-022235-6ircruag 174 27 and and CC cord-022235-6ircruag 174 28 hence hence RB cord-022235-6ircruag 174 29 directly directly RB cord-022235-6ircruag 174 30 or or CC cord-022235-6ircruag 174 31 indirectly indirectly RB cord-022235-6ircruag 174 32 assist assist VB cord-022235-6ircruag 174 33 vesicle vesicle NN cord-022235-6ircruag 174 34 formation formation NN cord-022235-6ircruag 174 35 and and CC cord-022235-6ircruag 174 36 protein protein NN cord-022235-6ircruag 174 37 transport transport NN cord-022235-6ircruag 174 38 to to IN cord-022235-6ircruag 174 39 the the DT cord-022235-6ircruag 174 40 Golgi Golgi NNP cord-022235-6ircruag 174 41 . . . cord-022235-6ircruag 175 1 Secretory secretory JJ cord-022235-6ircruag 175 2 pathway pathway NN cord-022235-6ircruag 175 3 " " `` cord-022235-6ircruag 175 4 shuttle shuttle NN cord-022235-6ircruag 175 5 " " '' cord-022235-6ircruag 175 6 vesicles vesicle NNS cord-022235-6ircruag 175 7 are be VBP cord-022235-6ircruag 175 8 difficult difficult JJ cord-022235-6ircruag 175 9 to to TO cord-022235-6ircruag 175 10 isolate isolate VB cord-022235-6ircruag 175 11 because because IN cord-022235-6ircruag 175 12 ( ( -LRB- cord-022235-6ircruag 175 13 533 533 CD cord-022235-6ircruag 175 14 ) ) -RRB- cord-022235-6ircruag 175 15 also also RB cord-022235-6ircruag 175 16 showed show VBD cord-022235-6ircruag 175 17 that that IN cord-022235-6ircruag 175 18 ATP ATP NNP cord-022235-6ircruag 175 19 depletion depletion NN cord-022235-6ircruag 175 20 caused cause VBD cord-022235-6ircruag 175 21 secretory secretory NN cord-022235-6ircruag 175 22 pro pro FW cord-022235-6ircruag 175 23 teins tein NNS cord-022235-6ircruag 175 24 to to TO cord-022235-6ircruag 175 25 accumulate accumulate VB cord-022235-6ircruag 175 26 in in IN cord-022235-6ircruag 175 27 transitional transitional JJ cord-022235-6ircruag 175 28 elements element NNS cord-022235-6ircruag 175 29 , , , cord-022235-6ircruag 175 30 but but CC cord-022235-6ircruag 175 31 this this DT cord-022235-6ircruag 175 32 study study NN cord-022235-6ircruag 175 33 did do VBD cord-022235-6ircruag 175 34 not not RB cord-022235-6ircruag 175 35 distin distin VB cord-022235-6ircruag 175 36 guish guish NNP cord-022235-6ircruag 175 37 between between IN cord-022235-6ircruag 175 38 energy energy NN cord-022235-6ircruag 175 39 requirements requirement NNS cord-022235-6ircruag 175 40 for for IN cord-022235-6ircruag 175 41 protein protein NN cord-022235-6ircruag 175 42 folding folding NN cord-022235-6ircruag 175 43 and and CC cord-022235-6ircruag 175 44 for for IN cord-022235-6ircruag 175 45 vesicular vesicular JJ cord-022235-6ircruag 175 46 transport transport NN cord-022235-6ircruag 175 47 from from IN cord-022235-6ircruag 175 48 the the DT cord-022235-6ircruag 175 49 transitional transitional JJ cord-022235-6ircruag 175 50 element element NN cord-022235-6ircruag 175 51 to to IN cord-022235-6ircruag 175 52 the the DT cord-022235-6ircruag 175 53 Golgi Golgi NNP cord-022235-6ircruag 175 54 . . . cord-022235-6ircruag 176 1 A a DT cord-022235-6ircruag 176 2 different different JJ cord-022235-6ircruag 176 3 in in IN cord-022235-6ircruag 176 4 vitro vitro FW cord-022235-6ircruag 176 5 assay assay NN cord-022235-6ircruag 176 6 was be VBD cord-022235-6ircruag 176 7 developed develop VBN cord-022235-6ircruag 176 8 by by IN cord-022235-6ircruag 176 9 Haselbeck Haselbeck NNP cord-022235-6ircruag 176 10 and and CC cord-022235-6ircruag 176 11 Schekman Schekman NNP cord-022235-6ircruag 176 12 ( ( -LRB- cord-022235-6ircruag 176 13 428 428 CD cord-022235-6ircruag 176 14 ) ) -RRB- cord-022235-6ircruag 176 15 to to TO cord-022235-6ircruag 176 16 study study VB cord-022235-6ircruag 176 17 protein protein NN cord-022235-6ircruag 176 18 movement movement NN cord-022235-6ircruag 176 19 from from IN cord-022235-6ircruag 176 20 the the DT cord-022235-6ircruag 176 21 ER ER NNP cord-022235-6ircruag 176 22 to to IN cord-022235-6ircruag 176 23 the the DT cord-022235-6ircruag 176 24 Golgi Golgi NNP cord-022235-6ircruag 176 25 in in IN cord-022235-6ircruag 176 26 yeast yeast NN cord-022235-6ircruag 176 27 cell cell NN cord-022235-6ircruag 176 28 extracts extract NNS cord-022235-6ircruag 176 29 . . . cord-022235-6ircruag 177 1 Their -PRON- PRP$ cord-022235-6ircruag 177 2 assay assay NN cord-022235-6ircruag 177 3 uses use VBZ cord-022235-6ircruag 177 4 donor donor NN cord-022235-6ircruag 177 5 ER er NN cord-022235-6ircruag 177 6 vesicles vesicle NNS cord-022235-6ircruag 177 7 derived derive VBN cord-022235-6ircruag 177 8 from from IN cord-022235-6ircruag 177 9 a a DT cord-022235-6ircruag 177 10 strain strain NN cord-022235-6ircruag 177 11 carry carry VBP cord-022235-6ircruag 177 12 ing e VBG cord-022235-6ircruag 177 13 the the DT cord-022235-6ircruag 177 14 secl8ts secl8ts NNP cord-022235-6ircruag 177 15 mutation mutation NN cord-022235-6ircruag 177 16 , , , cord-022235-6ircruag 177 17 which which WDT cord-022235-6ircruag 177 18 blocks block VBZ cord-022235-6ircruag 177 19 protein protein NN cord-022235-6ircruag 177 20 movement movement NN cord-022235-6ircruag 177 21 from from IN cord-022235-6ircruag 177 22 the the DT cord-022235-6ircruag 177 23 ER ER NNP cord-022235-6ircruag 177 24 , , , cord-022235-6ircruag 177 25 and and CC cord-022235-6ircruag 177 26 the the DT cord-022235-6ircruag 177 27 mnnl mnnl NNP cord-022235-6ircruag 177 28 mutation mutation NN cord-022235-6ircruag 177 29 , , , cord-022235-6ircruag 177 30 which which WDT cord-022235-6ircruag 177 31 prevents prevent VBZ cord-022235-6ircruag 177 32 terminal terminal JJ cord-022235-6ircruag 177 33 ( ( -LRB- cord-022235-6ircruag 177 34 Golgi Golgi NNP cord-022235-6ircruag 177 35 ) ) -RRB- cord-022235-6ircruag 177 36 mannosylation mannosylation NN cord-022235-6ircruag 177 37 of of IN cord-022235-6ircruag 177 38 secretory secretory JJ cord-022235-6ircruag 177 39 proteins protein NNS cord-022235-6ircruag 177 40 . . . cord-022235-6ircruag 178 1 Invertase Invertase NNP cord-022235-6ircruag 178 2 accumulated accumulate VBD cord-022235-6ircruag 178 3 in in IN cord-022235-6ircruag 178 4 the the DT cord-022235-6ircruag 178 5 ER ER NNP cord-022235-6ircruag 178 6 grown grow VBN cord-022235-6ircruag 178 7 at at IN cord-022235-6ircruag 178 8 the the DT cord-022235-6ircruag 178 9 restric restric JJ cord-022235-6ircruag 178 10 tive tive JJ cord-022235-6ircruag 178 11 temperature temperature NN cord-022235-6ircruag 178 12 was be VBD cord-022235-6ircruag 178 13 mannosylated mannosylate VBN cord-022235-6ircruag 178 14 after after IN cord-022235-6ircruag 178 15 transfer transfer NN cord-022235-6ircruag 178 16 to to IN cord-022235-6ircruag 178 17 recipient recipient NN cord-022235-6ircruag 178 18 Golgi Golgi NNP cord-022235-6ircruag 178 19 from from IN cord-022235-6ircruag 178 20 wild wild JJ cord-022235-6ircruag 178 21 - - HYPH cord-022235-6ircruag 178 22 type type NN cord-022235-6ircruag 178 23 cells cell NNS cord-022235-6ircruag 178 24 . . . cord-022235-6ircruag 179 1 The the DT cord-022235-6ircruag 179 2 transfer transfer NN cord-022235-6ircruag 179 3 efficiency efficiency NN cord-022235-6ircruag 179 4 was be VBD cord-022235-6ircruag 179 5 low low JJ cord-022235-6ircruag 179 6 , , , cord-022235-6ircruag 179 7 however however RB cord-022235-6ircruag 179 8 , , , cord-022235-6ircruag 179 9 possibly possibly RB cord-022235-6ircruag 179 10 be be VB cord-022235-6ircruag 179 11 cause cause IN cord-022235-6ircruag 179 12 invertase invertase NNP cord-022235-6ircruag 179 13 accumulated accumulate VBN cord-022235-6ircruag 179 14 at at IN cord-022235-6ircruag 179 15 the the DT cord-022235-6ircruag 179 16 restrictive restrictive JJ cord-022235-6ircruag 179 17 temperature temperature NN cord-022235-6ircruag 180 1 did do VBD cord-022235-6ircruag 180 2 not not RB cord-022235-6ircruag 180 3 be be VB cord-022235-6ircruag 180 4 come come VBN cord-022235-6ircruag 180 5 exit exit NN cord-022235-6ircruag 180 6 - - HYPH cord-022235-6ircruag 180 7 competent competent JJ cord-022235-6ircruag 180 8 at at IN cord-022235-6ircruag 180 9 the the DT cord-022235-6ircruag 180 10 permissive permissive JJ cord-022235-6ircruag 180 11 temperature temperature NN cord-022235-6ircruag 180 12 in in IN cord-022235-6ircruag 180 13 vitro vitro FW cord-022235-6ircruag 180 14 , , , cord-022235-6ircruag 180 15 or or CC cord-022235-6ircruag 180 16 because because IN cord-022235-6ircruag 180 17 recipient recipient NN cord-022235-6ircruag 180 18 Golgi Golgi NNP cord-022235-6ircruag 180 19 were be VBD cord-022235-6ircruag 180 20 saturated saturate VBN cord-022235-6ircruag 180 21 . . . cord-022235-6ircruag 181 1 As as IN cord-022235-6ircruag 181 2 in in IN cord-022235-6ircruag 181 3 the the DT cord-022235-6ircruag 181 4 mammalian mammalian JJ cord-022235-6ircruag 181 5 system system NN cord-022235-6ircruag 181 6 , , , cord-022235-6ircruag 181 7 protein protein NN cord-022235-6ircruag 181 8 transfer transfer NN cord-022235-6ircruag 181 9 was be VBD cord-022235-6ircruag 181 10 ATP ATP NNP cord-022235-6ircruag 181 11 - - HYPH cord-022235-6ircruag 181 12 dependent dependent JJ cord-022235-6ircruag 181 13 and and CC cord-022235-6ircruag 181 14 required require VBD cord-022235-6ircruag 181 15 soluble soluble JJ cord-022235-6ircruag 181 16 cofactors cofactor NNS cord-022235-6ircruag 181 17 including include VBG cord-022235-6ircruag 181 18 SEC SEC NNP cord-022235-6ircruag 181 19 18 18 CD cord-022235-6ircruag 181 20 protein protein NN cord-022235-6ircruag 181 21 ( ( -LRB- cord-022235-6ircruag 181 22 269b 269b CD cord-022235-6ircruag 181 23 ) ) -RRB- cord-022235-6ircruag 181 24 as as RB cord-022235-6ircruag 181 25 well well RB cord-022235-6ircruag 181 26 as as IN cord-022235-6ircruag 181 27 proteins protein NNS cord-022235-6ircruag 181 28 on on IN cord-022235-6ircruag 181 29 the the DT cord-022235-6ircruag 181 30 surface surface NN cord-022235-6ircruag 181 31 of of IN cord-022235-6ircruag 181 32 the the DT cord-022235-6ircruag 181 33 recipient recipient NN cord-022235-6ircruag 181 34 Golgi Golgi NNP cord-022235-6ircruag 181 35 . . . cord-022235-6ircruag 182 1 A a DT cord-022235-6ircruag 182 2 similar similar JJ cord-022235-6ircruag 182 3 but but CC cord-022235-6ircruag 182 4 more more RBR cord-022235-6ircruag 182 5 efficient efficient JJ cord-022235-6ircruag 182 6 reconstitution reconstitution NN cord-022235-6ircruag 182 7 system system NN cord-022235-6ircruag 182 8 using use VBG cord-022235-6ircruag 182 9 gently gently RB cord-022235-6ircruag 182 10 lysed lyse VBN cord-022235-6ircruag 182 11 yeast yeast NN cord-022235-6ircruag 182 12 - - HYPH cord-022235-6ircruag 182 13 cells cell NNS cord-022235-6ircruag 182 14 has have VBZ cord-022235-6ircruag 182 15 recently recently RB cord-022235-6ircruag 182 16 been be VBN cord-022235-6ircruag 182 17 developed develop VBN cord-022235-6ircruag 182 18 ( ( -LRB- cord-022235-6ircruag 182 19 35a 35a NNS cord-022235-6ircruag 182 20 ) ) -RRB- cord-022235-6ircruag 182 21 . . . cord-022235-6ircruag 183 1 Results result NNS cord-022235-6ircruag 183 2 obtained obtain VBN cord-022235-6ircruag 183 3 with with IN cord-022235-6ircruag 183 4 this this DT cord-022235-6ircruag 183 5 system system NN cord-022235-6ircruag 183 6 indicate indicate VBP cord-022235-6ircruag 183 7 the the DT cord-022235-6ircruag 183 8 probable probable JJ cord-022235-6ircruag 183 9 requirement requirement NN cord-022235-6ircruag 183 10 for for IN cord-022235-6ircruag 183 11 GTP GTP NNP cord-022235-6ircruag 183 12 in in IN cord-022235-6ircruag 183 13 ER ER NNP cord-022235-6ircruag 183 14 to to IN cord-022235-6ircruag 183 15 Golgi Golgi NNP cord-022235-6ircruag 183 16 traffic traffic NN cord-022235-6ircruag 183 17 . . . cord-022235-6ircruag 184 1 Secretory secretory JJ cord-022235-6ircruag 184 2 proteins protein NNS cord-022235-6ircruag 184 3 are be VBP cord-022235-6ircruag 184 4 subjected subject VBN cord-022235-6ircruag 184 5 to to IN cord-022235-6ircruag 184 6 further further JJ cord-022235-6ircruag 184 7 chemical chemical JJ cord-022235-6ircruag 184 8 modification modification NN cord-022235-6ircruag 184 9 as as IN cord-022235-6ircruag 184 10 they -PRON- PRP cord-022235-6ircruag 184 11 transit transit VBP cord-022235-6ircruag 184 12 through through IN cord-022235-6ircruag 184 13 the the DT cord-022235-6ircruag 184 14 Golgi Golgi NNP cord-022235-6ircruag 184 15 . . . cord-022235-6ircruag 185 1 These these DT cord-022235-6ircruag 185 2 processes process NNS cord-022235-6ircruag 185 3 are be VBP cord-022235-6ircruag 185 4 discussed discuss VBN cord-022235-6ircruag 185 5 in in IN cord-022235-6ircruag 185 6 the the DT cord-022235-6ircruag 185 7 following follow VBG cord-022235-6ircruag 185 8 sections section NNS cord-022235-6ircruag 185 9 ( ( -LRB- cord-022235-6ircruag 185 10 except except IN cord-022235-6ircruag 185 11 palmitoylation palmitoylation NNP cord-022235-6ircruag 185 12 , , , cord-022235-6ircruag 185 13 which which WDT cord-022235-6ircruag 185 14 was be VBD cord-022235-6ircruag 185 15 covered cover VBN cord-022235-6ircruag 185 16 in in IN cord-022235-6ircruag 185 17 section section NN cord-022235-6ircruag 185 18 III.F.4.c III.F.4.c NNP cord-022235-6ircruag 185 19 ) ) -RRB- cord-022235-6ircruag 185 20 ; ; : cord-022235-6ircruag 185 21 their -PRON- PRP$ cord-022235-6ircruag 185 22 significance significance NN cord-022235-6ircruag 185 23 with with IN cord-022235-6ircruag 185 24 regards regard NNS cord-022235-6ircruag 185 25 to to IN cord-022235-6ircruag 185 26 compartmentalization compartmentalization NN cord-022235-6ircruag 185 27 of of IN cord-022235-6ircruag 185 28 the the DT cord-022235-6ircruag 185 29 Golgi Golgi NNP cord-022235-6ircruag 185 30 appa appa NNP cord-022235-6ircruag 185 31 ratus ratus NNP cord-022235-6ircruag 185 32 and and CC cord-022235-6ircruag 185 33 their -PRON- PRP$ cord-022235-6ircruag 185 34 role role NN cord-022235-6ircruag 185 35 in in IN cord-022235-6ircruag 185 36 protein protein NN cord-022235-6ircruag 185 37 targeting targeting NN cord-022235-6ircruag 185 38 will will MD cord-022235-6ircruag 185 39 be be VB cord-022235-6ircruag 185 40 discussed discuss VBN cord-022235-6ircruag 185 41 in in IN cord-022235-6ircruag 185 42 later later JJ cord-022235-6ircruag 185 43 sections section NNS cord-022235-6ircruag 185 44 of of IN cord-022235-6ircruag 185 45 this this DT cord-022235-6ircruag 185 46 chapter chapter NN cord-022235-6ircruag 185 47 . . . cord-022235-6ircruag 186 1 In in IN cord-022235-6ircruag 186 2 Section section NN cord-022235-6ircruag 186 3 V.B.I V.B.I NNP cord-022235-6ircruag 186 4 , , , cord-022235-6ircruag 186 5 we -PRON- PRP cord-022235-6ircruag 186 6 saw see VBD cord-022235-6ircruag 186 7 that that IN cord-022235-6ircruag 186 8 the the DT cord-022235-6ircruag 186 9 basic basic JJ cord-022235-6ircruag 186 10 oligosaccharide oligosaccharide NN cord-022235-6ircruag 186 11 core core NN cord-022235-6ircruag 186 12 on on IN cord-022235-6ircruag 186 13 Asn Asn NNP cord-022235-6ircruag 186 14 residues residue NNS cord-022235-6ircruag 186 15 is be VBZ cord-022235-6ircruag 186 16 trimmed trim VBN cord-022235-6ircruag 186 17 by by IN cord-022235-6ircruag 186 18 glucose glucose NN cord-022235-6ircruag 186 19 - - HYPH cord-022235-6ircruag 186 20 and and CC cord-022235-6ircruag 186 21 mannosidases mannosidase NNS cord-022235-6ircruag 186 22 before before IN cord-022235-6ircruag 186 23 secretory secretory JJ cord-022235-6ircruag 186 24 pro pro FW cord-022235-6ircruag 186 25 teins tein NNS cord-022235-6ircruag 186 26 leave leave VBP cord-022235-6ircruag 186 27 the the DT cord-022235-6ircruag 186 28 ER er NN cord-022235-6ircruag 186 29 . . . cord-022235-6ircruag 187 1 Following follow VBG cord-022235-6ircruag 187 2 their -PRON- PRP$ cord-022235-6ircruag 187 3 arrival arrival NN cord-022235-6ircruag 187 4 in in IN cord-022235-6ircruag 187 5 the the DT cord-022235-6ircruag 187 6 Golgi Golgi NNP cord-022235-6ircruag 187 7 , , , cord-022235-6ircruag 187 8 mannose mannose NN cord-022235-6ircruag 187 9 residues residue NNS cord-022235-6ircruag 187 10 on on IN cord-022235-6ircruag 187 11 secretory secretory JJ cord-022235-6ircruag 187 12 proteins protein NNS cord-022235-6ircruag 187 13 may may MD cord-022235-6ircruag 187 14 be be VB cord-022235-6ircruag 187 15 processed process VBN cord-022235-6ircruag 187 16 in in IN cord-022235-6ircruag 187 17 one one CD cord-022235-6ircruag 187 18 of of IN cord-022235-6ircruag 187 19 two two CD cord-022235-6ircruag 187 20 ways way NNS cord-022235-6ircruag 187 21 , , , cord-022235-6ircruag 187 22 depending depend VBG cord-022235-6ircruag 187 23 on on IN cord-022235-6ircruag 187 24 whether whether IN cord-022235-6ircruag 187 25 they -PRON- PRP cord-022235-6ircruag 187 26 are be VBP cord-022235-6ircruag 187 27 targeted target VBN cord-022235-6ircruag 187 28 to to IN cord-022235-6ircruag 187 29 the the DT cord-022235-6ircruag 187 30 lysosome lysosome NN cord-022235-6ircruag 187 31 . . . cord-022235-6ircruag 188 1 Soluble soluble JJ cord-022235-6ircruag 188 2 lysosomal lysosomal JJ cord-022235-6ircruag 188 3 proteins protein NNS cord-022235-6ircruag 188 4 carry carry VBP cord-022235-6ircruag 188 5 phosphorylated phosphorylated JJ cord-022235-6ircruag 188 6 mannose mannose NN cord-022235-6ircruag 188 7 residues residue NNS cord-022235-6ircruag 188 8 which which WDT cord-022235-6ircruag 188 9 function function VBP cord-022235-6ircruag 188 10 as as IN cord-022235-6ircruag 188 11 lysosomal lysosomal JJ cord-022235-6ircruag 188 12 sorting sorting NN cord-022235-6ircruag 188 13 signals signal NNS cord-022235-6ircruag 188 14 ( ( -LRB- cord-022235-6ircruag 188 15 Section section NN cord-022235-6ircruag 188 16 V.G.5 V.G.5 NNP cord-022235-6ircruag 188 17 ) ) -RRB- cord-022235-6ircruag 188 18 . . . cord-022235-6ircruag 189 1 They -PRON- PRP cord-022235-6ircruag 189 2 un un NNP cord-022235-6ircruag 189 3 dergo dergo VB cord-022235-6ircruag 189 4 specific specific JJ cord-022235-6ircruag 189 5 mannose mannose NN cord-022235-6ircruag 189 6 phosphorylation phosphorylation NN cord-022235-6ircruag 190 1 catalyzed catalyze VBN cord-022235-6ircruag 190 2 by by IN cord-022235-6ircruag 190 3 one one CD cord-022235-6ircruag 190 4 or or CC cord-022235-6ircruag 190 5 two two CD cord-022235-6ircruag 190 6 Nacetylglucosamylphosphotransferases Nacetylglucosamylphosphotransferases NNPS cord-022235-6ircruag 190 7 and and CC cord-022235-6ircruag 190 8 7V 7v CD cord-022235-6ircruag 190 9 - - HYPH cord-022235-6ircruag 190 10 acetylglucosamine-1 acetylglucosamine-1 NN cord-022235-6ircruag 191 1 -phosphodiester -phosphodiester : cord-022235-6ircruag 191 2 - - HYPH cord-022235-6ircruag 191 3 a a DT cord-022235-6ircruag 191 4 - - HYPH cord-022235-6ircruag 191 5 N n NN cord-022235-6ircruag 191 6 - - HYPH cord-022235-6ircruag 191 7 acetylglucosaminidase acetylglucosaminidase NNP cord-022235-6ircruag 191 8 ( ( -LRB- cord-022235-6ircruag 191 9 phosphodiesterase phosphodiesterase NN cord-022235-6ircruag 191 10 ) ) -RRB- cord-022235-6ircruag 191 11 in in IN cord-022235-6ircruag 191 12 different different JJ cord-022235-6ircruag 191 13 cis cis NNP cord-022235-6ircruag 191 14 Golgi Golgi NNP cord-022235-6ircruag 191 15 compartments compartment NNS cord-022235-6ircruag 191 16 ( ( -LRB- cord-022235-6ircruag 191 17 614a 614a NNP cord-022235-6ircruag 191 18 ) ) -RRB- cord-022235-6ircruag 191 19 . . . cord-022235-6ircruag 192 1 Sequential sequential JJ cord-022235-6ircruag 192 2 action action NN cord-022235-6ircruag 192 3 of of IN cord-022235-6ircruag 192 4 these these DT cord-022235-6ircruag 192 5 enzymes enzyme NNS cord-022235-6ircruag 192 6 results result NNS cord-022235-6ircruag 192 7 in in IN cord-022235-6ircruag 192 8 the the DT cord-022235-6ircruag 192 9 transfer transfer NN cord-022235-6ircruag 192 10 of of IN cord-022235-6ircruag 192 11 TV tv NN cord-022235-6ircruag 192 12 - - HYPH cord-022235-6ircruag 192 13 acetyl acetyl NN cord-022235-6ircruag 192 14 glucosamine-1-phosphate glucosamine-1-phosphate NN cord-022235-6ircruag 192 15 from from IN cord-022235-6ircruag 192 16 UDP udp NN cord-022235-6ircruag 192 17 - - HYPH cord-022235-6ircruag 192 18 NAGn nagn NN cord-022235-6ircruag 192 19 to to IN cord-022235-6ircruag 192 20 any any DT cord-022235-6ircruag 192 21 one one CD cord-022235-6ircruag 192 22 of of IN cord-022235-6ircruag 192 23 five five CD cord-022235-6ircruag 192 24 mannose mannose NN cord-022235-6ircruag 192 25 residues residue NNS cord-022235-6ircruag 192 26 in in IN cord-022235-6ircruag 192 27 the the DT cord-022235-6ircruag 192 28 oligosaccharide oligosaccharide NN cord-022235-6ircruag 192 29 core core NN cord-022235-6ircruag 192 30 , , , cord-022235-6ircruag 192 31 followed follow VBD cord-022235-6ircruag 192 32 shortly shortly RB cord-022235-6ircruag 192 33 afterwards afterwards RB cord-022235-6ircruag 192 34 by by IN cord-022235-6ircruag 192 35 the the DT cord-022235-6ircruag 192 36 removal removal NN cord-022235-6ircruag 192 37 of of IN cord-022235-6ircruag 192 38 the the DT cord-022235-6ircruag 192 39 jV jV NNP cord-022235-6ircruag 192 40 - - HYPH cord-022235-6ircruag 192 41 acetylglucosamine acetylglucosamine NN cord-022235-6ircruag 192 42 to to TO cord-022235-6ircruag 192 43 expose expose VB cord-022235-6ircruag 192 44 the the DT cord-022235-6ircruag 192 45 phosphomannosyl phosphomannosyl JJ cord-022235-6ircruag 192 46 group group NN cord-022235-6ircruag 192 47 ( ( -LRB- cord-022235-6ircruag 192 48 577 577 CD cord-022235-6ircruag 192 49 ) ) -RRB- cord-022235-6ircruag 192 50 . . . cord-022235-6ircruag 193 1 Goldberg Goldberg NNP cord-022235-6ircruag 193 2 and and CC cord-022235-6ircruag 193 3 Kornfeld Kornfeld NNP cord-022235-6ircruag 193 4 ( ( -LRB- cord-022235-6ircruag 193 5 379 379 CD cord-022235-6ircruag 193 6 ) ) -RRB- cord-022235-6ircruag 193 7 found find VBD cord-022235-6ircruag 193 8 three three CD cord-022235-6ircruag 193 9 partially partially RB cord-022235-6ircruag 193 10 phosphorylated phosphorylate VBN cord-022235-6ircruag 193 11 peptides peptide NNS cord-022235-6ircruag 193 12 in in IN cord-022235-6ircruag 193 13 β β NN cord-022235-6ircruag 193 14 - - HYPH cord-022235-6ircruag 193 15 glucuronidase glucuronidase NNP cord-022235-6ircruag 193 16 , , , cord-022235-6ircruag 193 17 indicating indicate VBG cord-022235-6ircruag 193 18 that that IN cord-022235-6ircruag 193 19 lysosomal lysosomal JJ cord-022235-6ircruag 193 20 enzymes enzyme NNS cord-022235-6ircruag 193 21 may may MD cord-022235-6ircruag 193 22 not not RB cord-022235-6ircruag 193 23 be be VB cord-022235-6ircruag 193 24 uniformly uniformly RB cord-022235-6ircruag 193 25 phosphorylated phosphorylate VBN cord-022235-6ircruag 193 26 . . . cord-022235-6ircruag 194 1 Lysosomal Lysosomal NNP cord-022235-6ircruag 194 2 enzymes enzyme NNS cord-022235-6ircruag 194 3 presumably presumably RB cord-022235-6ircruag 194 4 contain contain VBP cord-022235-6ircruag 194 5 signals signal NNS cord-022235-6ircruag 194 6 which which WDT cord-022235-6ircruag 194 7 are be VBP cord-022235-6ircruag 194 8 recognized recognize VBN cord-022235-6ircruag 194 9 by by IN cord-022235-6ircruag 194 10 the the DT cord-022235-6ircruag 194 11 phosphorylating phosphorylate VBG cord-022235-6ircruag 194 12 enzymes enzyme NNS cord-022235-6ircruag 194 13 ( ( -LRB- cord-022235-6ircruag 194 14 919 919 CD cord-022235-6ircruag 194 15 ) ) -RRB- cord-022235-6ircruag 194 16 . . . cord-022235-6ircruag 195 1 Deglycosylated Deglycosylated NNP cord-022235-6ircruag 195 2 ( ( -LRB- cord-022235-6ircruag 195 3 endoglycosidase endoglycosidase NNP cord-022235-6ircruag 195 4 Η η NN cord-022235-6ircruag 195 5 - - HYPH cord-022235-6ircruag 195 6 treated treat VBN cord-022235-6ircruag 195 7 ) ) -RRB- cord-022235-6ircruag 196 1 lysosomal lysosomal JJ cord-022235-6ircruag 196 2 cathepsin cathepsin NN cord-022235-6ircruag 196 3 D d NN cord-022235-6ircruag 196 4 is be VBZ cord-022235-6ircruag 196 5 not not RB cord-022235-6ircruag 196 6 a a DT cord-022235-6ircruag 196 7 substrate substrate NN cord-022235-6ircruag 196 8 for for IN cord-022235-6ircruag 196 9 the the DT cord-022235-6ircruag 196 10 phosphoryl phosphoryl NNP cord-022235-6ircruag 196 11 ation ation NN cord-022235-6ircruag 196 12 reaction reaction NN cord-022235-6ircruag 196 13 in in IN cord-022235-6ircruag 196 14 vitro vitro FW cord-022235-6ircruag 196 15 , , , cord-022235-6ircruag 196 16 but but CC cord-022235-6ircruag 196 17 it -PRON- PRP cord-022235-6ircruag 196 18 inhibits inhibit VBZ cord-022235-6ircruag 196 19 phosphorylation phosphorylation NN cord-022235-6ircruag 196 20 of of IN cord-022235-6ircruag 196 21 intact intact JJ cord-022235-6ircruag 196 22 lysosomal lysosomal JJ cord-022235-6ircruag 196 23 enzymes enzyme NNS cord-022235-6ircruag 196 24 . . . cord-022235-6ircruag 197 1 Proteolytic proteolytic JJ cord-022235-6ircruag 197 2 fragments fragment NNS cord-022235-6ircruag 197 3 of of IN cord-022235-6ircruag 197 4 glycosylated glycosylated JJ cord-022235-6ircruag 197 5 cathepsin cathepsin NN cord-022235-6ircruag 197 6 D d NN cord-022235-6ircruag 197 7 are be VBP cord-022235-6ircruag 197 8 also also RB cord-022235-6ircruag 197 9 not not RB cord-022235-6ircruag 197 10 phosphorylated phosphorylated JJ cord-022235-6ircruag 197 11 , , , cord-022235-6ircruag 197 12 and and CC cord-022235-6ircruag 197 13 do do VBP cord-022235-6ircruag 197 14 not not RB cord-022235-6ircruag 197 15 inhibit inhibit VB cord-022235-6ircruag 197 16 phosphorylation phosphorylation NN cord-022235-6ircruag 197 17 when when WRB cord-022235-6ircruag 197 18 they -PRON- PRP cord-022235-6ircruag 197 19 are be VBP cord-022235-6ircruag 197 20 dephosphorylated dephosphorylate VBN cord-022235-6ircruag 197 21 , , , cord-022235-6ircruag 197 22 indicating indicate VBG cord-022235-6ircruag 197 23 that that IN cord-022235-6ircruag 197 24 the the DT cord-022235-6ircruag 197 25 " " `` cord-022235-6ircruag 197 26 phosphorylation phosphorylation NN cord-022235-6ircruag 197 27 signal signal NN cord-022235-6ircruag 197 28 " " '' cord-022235-6ircruag 197 29 is be VBZ cord-022235-6ircruag 197 30 probably probably RB cord-022235-6ircruag 197 31 not not RB cord-022235-6ircruag 197 32 a a DT cord-022235-6ircruag 197 33 linear linear JJ cord-022235-6ircruag 197 34 sequence sequence NN cord-022235-6ircruag 197 35 of of IN cord-022235-6ircruag 197 36 amino amino JJ cord-022235-6ircruag 197 37 acids acid NNS cord-022235-6ircruag 197 38 ( ( -LRB- cord-022235-6ircruag 197 39 612 612 CD cord-022235-6ircruag 197 40 ) ) -RRB- cord-022235-6ircruag 197 41 . . . cord-022235-6ircruag 198 1 Frog Frog NNP cord-022235-6ircruag 198 2 oocytes oocyte NNS cord-022235-6ircruag 198 3 are be VBP cord-022235-6ircruag 198 4 also also RB cord-022235-6ircruag 198 5 able able JJ cord-022235-6ircruag 198 6 to to TO cord-022235-6ircruag 198 7 recognize recognize VB cord-022235-6ircruag 198 8 the the DT cord-022235-6ircruag 198 9 phosphorylation phosphorylation NN cord-022235-6ircruag 198 10 signal signal NN cord-022235-6ircruag 198 11 of of IN cord-022235-6ircruag 198 12 human human JJ cord-022235-6ircruag 198 13 cathepsin cathepsin NN cord-022235-6ircruag 198 14 D d NN cord-022235-6ircruag 198 15 ( ( -LRB- cord-022235-6ircruag 198 16 301 301 CD cord-022235-6ircruag 198 17 ) ) -RRB- cord-022235-6ircruag 198 18 . . . cord-022235-6ircruag 199 1 Renin renin NN cord-022235-6ircruag 199 2 is be VBZ cord-022235-6ircruag 199 3 closely closely RB cord-022235-6ircruag 199 4 related related JJ cord-022235-6ircruag 199 5 to to IN cord-022235-6ircruag 199 6 cathepsin cathepsin NN cord-022235-6ircruag 199 7 D d NN cord-022235-6ircruag 199 8 and and CC cord-022235-6ircruag 199 9 yet yet RB cord-022235-6ircruag 199 10 is be VBZ cord-022235-6ircruag 199 11 secreted secrete VBN cord-022235-6ircruag 199 12 by by IN cord-022235-6ircruag 199 13 mammalian mammalian JJ cord-022235-6ircruag 199 14 cells cell NNS cord-022235-6ircruag 199 15 , , , cord-022235-6ircruag 199 16 presumably presumably RB cord-022235-6ircruag 199 17 because because IN cord-022235-6ircruag 199 18 it -PRON- PRP cord-022235-6ircruag 199 19 lacks lack VBZ cord-022235-6ircruag 199 20 the the DT cord-022235-6ircruag 199 21 phosphorylation phosphorylation NN cord-022235-6ircruag 199 22 signal signal NN cord-022235-6ircruag 199 23 . . . cord-022235-6ircruag 200 1 However however RB cord-022235-6ircruag 200 2 , , , cord-022235-6ircruag 200 3 renin renin NN cord-022235-6ircruag 200 4 produced produce VBN cord-022235-6ircruag 200 5 in in IN cord-022235-6ircruag 200 6 oocytes oocyte NNS cord-022235-6ircruag 200 7 is be VBZ cord-022235-6ircruag 200 8 phosphorylated phosphorylate VBN cord-022235-6ircruag 200 9 , , , cord-022235-6ircruag 200 10 remains remain VBZ cord-022235-6ircruag 200 11 intracellular intracellular JJ cord-022235-6ircruag 200 12 , , , cord-022235-6ircruag 200 13 and and CC cord-022235-6ircruag 200 14 is be VBZ cord-022235-6ircruag 200 15 de de FW cord-022235-6ircruag 200 16 graded grade VBN cord-022235-6ircruag 200 17 ( ( -LRB- cord-022235-6ircruag 200 18 presumably presumably RB cord-022235-6ircruag 200 19 in in IN cord-022235-6ircruag 200 20 the the DT cord-022235-6ircruag 200 21 lysosome lysosome NN cord-022235-6ircruag 200 22 ) ) -RRB- cord-022235-6ircruag 200 23 ( ( -LRB- cord-022235-6ircruag 200 24 302 302 CD cord-022235-6ircruag 200 25 ) ) -RRB- cord-022235-6ircruag 200 26 . . . cord-022235-6ircruag 201 1 This this DT cord-022235-6ircruag 201 2 suggests suggest VBZ cord-022235-6ircruag 201 3 that that IN cord-022235-6ircruag 201 4 renin renin NN cord-022235-6ircruag 201 5 has have VBZ cord-022235-6ircruag 201 6 a a DT cord-022235-6ircruag 201 7 phosphorylation phosphorylation NN cord-022235-6ircruag 201 8 signal signal NN cord-022235-6ircruag 201 9 which which WDT cord-022235-6ircruag 201 10 is be VBZ cord-022235-6ircruag 201 11 recognized recognize VBN cord-022235-6ircruag 201 12 by by IN cord-022235-6ircruag 201 13 amphibian amphibian NN cord-022235-6ircruag 201 14 but but CC cord-022235-6ircruag 201 15 not not RB cord-022235-6ircruag 201 16 by by IN cord-022235-6ircruag 201 17 mam mam NN cord-022235-6ircruag 201 18 malian malian NNP cord-022235-6ircruag 201 19 phosphorylating phosphorylate VBG cord-022235-6ircruag 201 20 enzymes enzyme NNS cord-022235-6ircruag 201 21 . . . cord-022235-6ircruag 202 1 Outer outer JJ cord-022235-6ircruag 202 2 chains chain NNS cord-022235-6ircruag 202 3 on on IN cord-022235-6ircruag 202 4 lysosomal lysosomal JJ cord-022235-6ircruag 202 5 and and CC cord-022235-6ircruag 202 6 nonlysosomal nonlysosomal JJ cord-022235-6ircruag 202 7 secretory secretory JJ cord-022235-6ircruag 202 8 proteins protein NNS cord-022235-6ircruag 202 9 of of IN cord-022235-6ircruag 202 10 com com NN cord-022235-6ircruag 202 11 plex plex NNP cord-022235-6ircruag 202 12 eukaryotes eukaryote NNS cord-022235-6ircruag 202 13 may may MD cord-022235-6ircruag 202 14 be be VB cord-022235-6ircruag 202 15 further further RB cord-022235-6ircruag 202 16 trimmed trim VBN cord-022235-6ircruag 202 17 by by IN cord-022235-6ircruag 202 18 Golgi Golgi NNP cord-022235-6ircruag 202 19 mannosidase mannosidase NN cord-022235-6ircruag 202 20 I -PRON- PRP cord-022235-6ircruag 202 21 to to TO cord-022235-6ircruag 202 22 leave leave VB cord-022235-6ircruag 202 23 five five CD cord-022235-6ircruag 202 24 mannose mannose NN cord-022235-6ircruag 202 25 residues residue NNS cord-022235-6ircruag 202 26 on on IN cord-022235-6ircruag 202 27 the the DT cord-022235-6ircruag 202 28 core core NN cord-022235-6ircruag 202 29 oligosaccharide oligosaccharide NN cord-022235-6ircruag 202 30 . . . cord-022235-6ircruag 203 1 Further further JJ cord-022235-6ircruag 203 2 modifications modification NNS cord-022235-6ircruag 203 3 involve involve VBP cord-022235-6ircruag 203 4 the the DT cord-022235-6ircruag 203 5 addition addition NN cord-022235-6ircruag 203 6 of of IN cord-022235-6ircruag 203 7 an an DT cord-022235-6ircruag 203 8 N n CD cord-022235-6ircruag 203 9 - - HYPH cord-022235-6ircruag 203 10 acetylglucosamine acetylglucosamine NN cord-022235-6ircruag 203 11 residue residue NN cord-022235-6ircruag 203 12 by by IN cord-022235-6ircruag 203 13 N N NNP cord-022235-6ircruag 203 14 - - NN cord-022235-6ircruag 203 15 acetylglucosaminyltransferase acetylglucosaminyltransferase NNP cord-022235-6ircruag 203 16 I -PRON- PRP cord-022235-6ircruag 203 17 , , , cord-022235-6ircruag 203 18 further further JJ cord-022235-6ircruag 203 19 removal removal NN cord-022235-6ircruag 203 20 of of IN cord-022235-6ircruag 203 21 two two CD cord-022235-6ircruag 203 22 mannose mannose NN cord-022235-6ircruag 203 23 residues residue NNS cord-022235-6ircruag 203 24 by by IN cord-022235-6ircruag 203 25 Golgi Golgi NNP cord-022235-6ircruag 203 26 mannosidase mannosidase NN cord-022235-6ircruag 203 27 II II NNP cord-022235-6ircruag 203 28 , , , cord-022235-6ircruag 203 29 fucosylation fucosylation NN cord-022235-6ircruag 203 30 of of IN cord-022235-6ircruag 203 31 the the DT cord-022235-6ircruag 203 32 innermost innermost JJ cord-022235-6ircruag 203 33 TV tv NN cord-022235-6ircruag 203 34 - - HYPH cord-022235-6ircruag 203 35 acetylglucosamine acetylglucosamine NN cord-022235-6ircruag 203 36 by by IN cord-022235-6ircruag 203 37 fucosyltransferase fucosyltransferase NN cord-022235-6ircruag 203 38 , , , cord-022235-6ircruag 203 39 and and CC cord-022235-6ircruag 203 40 the the DT cord-022235-6ircruag 203 41 addition addition NN cord-022235-6ircruag 203 42 of of IN cord-022235-6ircruag 203 43 galactose galactose NN cord-022235-6ircruag 203 44 , , , cord-022235-6ircruag 203 45 N N NNP cord-022235-6ircruag 203 46 - - HYPH cord-022235-6ircruag 203 47 acetylglucosamine acetylglucosamine NNP cord-022235-6ircruag 203 48 , , , cord-022235-6ircruag 203 49 and and CC cord-022235-6ircruag 203 50 sialic sialic JJ cord-022235-6ircruag 203 51 acid acid NN cord-022235-6ircruag 203 52 residues residue NNS cord-022235-6ircruag 203 53 by by IN cord-022235-6ircruag 203 54 appropriate appropriate JJ cord-022235-6ircruag 203 55 transferases transferase NNS cord-022235-6ircruag 203 56 ( ( -LRB- cord-022235-6ircruag 203 57 Fig fig NN cord-022235-6ircruag 203 58 . . . cord-022235-6ircruag 203 59 V. v. NN cord-022235-6ircruag 203 60 2 2 CD cord-022235-6ircruag 203 61 ) ) -RRB- cord-022235-6ircruag 203 62 . . . cord-022235-6ircruag 204 1 Oligosaccharides oligosaccharide NNS cord-022235-6ircruag 204 2 of of IN cord-022235-6ircruag 204 3 both both DT cord-022235-6ircruag 204 4 lysosomal lysosomal JJ cord-022235-6ircruag 204 5 and and CC cord-022235-6ircruag 204 6 nonlysosomal nonlysosomal JJ cord-022235-6ircruag 204 7 proteins protein NNS cord-022235-6ircruag 204 8 may may MD cord-022235-6ircruag 204 9 be be VB cord-022235-6ircruag 204 10 further further RB cord-022235-6ircruag 204 11 modified modify VBN cord-022235-6ircruag 204 12 by by IN cord-022235-6ircruag 204 13 sulfation sulfation NN cord-022235-6ircruag 204 14 of of IN cord-022235-6ircruag 204 15 mannose mannose NN cord-022235-6ircruag 204 16 and and CC cord-022235-6ircruag 204 17 N N NNP cord-022235-6ircruag 204 18 - - HYPH cord-022235-6ircruag 204 19 acetylglucosamine acetylglucosamine NNP cord-022235-6ircruag 204 20 resi resi JJ cord-022235-6ircruag 204 21 dues due NNS cord-022235-6ircruag 204 22 and and CC cord-022235-6ircruag 204 23 O o NN cord-022235-6ircruag 204 24 - - HYPH cord-022235-6ircruag 204 25 acetylation acetylation NN cord-022235-6ircruag 204 26 of of IN cord-022235-6ircruag 204 27 sialic sialic JJ cord-022235-6ircruag 204 28 acid acid NN cord-022235-6ircruag 204 29 residues residue NNS cord-022235-6ircruag 204 30 ( ( -LRB- cord-022235-6ircruag 204 31 577 577 CD cord-022235-6ircruag 204 32 ) ) -RRB- cord-022235-6ircruag 204 33 . . . cord-022235-6ircruag 205 1 Outer outer JJ cord-022235-6ircruag 205 2 chain chain NN cord-022235-6ircruag 205 3 modification modification NN cord-022235-6ircruag 205 4 in in IN cord-022235-6ircruag 205 5 yeasts yeast NNS cord-022235-6ircruag 205 6 is be VBZ cord-022235-6ircruag 205 7 markedly markedly RB cord-022235-6ircruag 205 8 different different JJ cord-022235-6ircruag 205 9 . . . cord-022235-6ircruag 206 1 Golgi Golgi NNP cord-022235-6ircruag 206 2 mannosyltransferases mannosyltransferase NNS cord-022235-6ircruag 206 3 extend extend VBP cord-022235-6ircruag 206 4 the the DT cord-022235-6ircruag 206 5 basic basic JJ cord-022235-6ircruag 206 6 ( ( -LRB- cord-022235-6ircruag 206 7 man man NN cord-022235-6ircruag 206 8 ) ) -RRB- cord-022235-6ircruag 206 9 8 8 CD cord-022235-6ircruag 206 10 core core NN cord-022235-6ircruag 206 11 oligosaccharide oligosaccharide NN cord-022235-6ircruag 206 12 to to TO cord-022235-6ircruag 206 13 produce produce VB cord-022235-6ircruag 206 14 large large JJ cord-022235-6ircruag 206 15 mannan mannan NN cord-022235-6ircruag 206 16 structures structure NNS cord-022235-6ircruag 206 17 typical typical JJ cord-022235-6ircruag 206 18 of of IN cord-022235-6ircruag 206 19 many many JJ cord-022235-6ircruag 206 20 yeast yeast NN cord-022235-6ircruag 206 21 mannoproteins mannoprotein NNS cord-022235-6ircruag 206 22 , , , cord-022235-6ircruag 206 23 which which WDT cord-022235-6ircruag 206 24 may may MD cord-022235-6ircruag 206 25 carry carry VB cord-022235-6ircruag 206 26 as as RB cord-022235-6ircruag 206 27 many many JJ cord-022235-6ircruag 206 28 as as IN cord-022235-6ircruag 206 29 150 150 CD cord-022235-6ircruag 206 30 mannose mannose NN cord-022235-6ircruag 206 31 residues residue NNS cord-022235-6ircruag 206 32 ( ( -LRB- cord-022235-6ircruag 206 33 598 598 CD cord-022235-6ircruag 206 34 ) ) -RRB- cord-022235-6ircruag 206 35 . . . cord-022235-6ircruag 207 1 The the DT cord-022235-6ircruag 207 2 single single JJ cord-022235-6ircruag 207 3 O o NN cord-022235-6ircruag 207 4 - - HYPH cord-022235-6ircruag 207 5 linked link VBN cord-022235-6ircruag 207 6 mannose mannose NN cord-022235-6ircruag 207 7 residue residue NN cord-022235-6ircruag 207 8 on on IN cord-022235-6ircruag 207 9 yeast yeast NN cord-022235-6ircruag 207 10 glycoproteins glycoprotein NNS cord-022235-6ircruag 207 11 ( ( -LRB- cord-022235-6ircruag 207 12 Section section NN cord-022235-6ircruag 207 13 V.B.l.c V.B.l.c NNP cord-022235-6ircruag 207 14 ) ) -RRB- cord-022235-6ircruag 207 15 may may MD cord-022235-6ircruag 207 16 be be VB cord-022235-6ircruag 207 17 further further RB cord-022235-6ircruag 207 18 modified modify VBN cord-022235-6ircruag 207 19 by by IN cord-022235-6ircruag 207 20 the the DT cord-022235-6ircruag 207 21 addition addition NN cord-022235-6ircruag 207 22 of of IN cord-022235-6ircruag 207 23 up up IN cord-022235-6ircruag 207 24 to to TO cord-022235-6ircruag 207 25 four four CD cord-022235-6ircruag 207 26 more more JJR cord-022235-6ircruag 207 27 mannoses mannose NNS cord-022235-6ircruag 207 28 transferred transfer VBN cord-022235-6ircruag 207 29 from from IN cord-022235-6ircruag 207 30 GDP gdp NN cord-022235-6ircruag 207 31 - - HYPH cord-022235-6ircruag 207 32 mannose mannose NN cord-022235-6ircruag 207 33 ( ( -LRB- cord-022235-6ircruag 207 34 598,1107 598,1107 CD cord-022235-6ircruag 207 35 blocked block VBN cord-022235-6ircruag 207 36 in in IN cord-022235-6ircruag 207 37 sec sec NNP cord-022235-6ircruag 207 38 mutants mutant NNS cord-022235-6ircruag 207 39 , , , cord-022235-6ircruag 207 40 which which WDT cord-022235-6ircruag 207 41 prevent prevent VBP cord-022235-6ircruag 207 42 secretory secretory NN cord-022235-6ircruag 207 43 proteins protein NNS cord-022235-6ircruag 207 44 from from IN cord-022235-6ircruag 207 45 reaching reach VBG cord-022235-6ircruag 207 46 the the DT cord-022235-6ircruag 207 47 Golgi Golgi NNP cord-022235-6ircruag 207 48 ( ( -LRB- cord-022235-6ircruag 207 49 429 429 CD cord-022235-6ircruag 207 50 ) ) -RRB- cord-022235-6ircruag 207 51 . . . cord-022235-6ircruag 208 1 In in IN cord-022235-6ircruag 208 2 complex complex JJ cord-022235-6ircruag 208 3 eukaryotes eukaryote NNS cord-022235-6ircruag 208 4 , , , cord-022235-6ircruag 208 5 hydroxyl hydroxyl NN cord-022235-6ircruag 208 6 groups group NNS cord-022235-6ircruag 208 7 of of IN cord-022235-6ircruag 208 8 Ser Ser NNP cord-022235-6ircruag 208 9 or or CC cord-022235-6ircruag 208 10 Thr Thr NNP cord-022235-6ircruag 208 11 residues residue NNS cord-022235-6ircruag 208 12 are be VBP cord-022235-6ircruag 208 13 Oglycosylated oglycosylate VBN cord-022235-6ircruag 208 14 by by IN cord-022235-6ircruag 208 15 enzymes enzyme NNS cord-022235-6ircruag 208 16 thought think VBN cord-022235-6ircruag 208 17 to to TO cord-022235-6ircruag 208 18 be be VB cord-022235-6ircruag 208 19 located locate VBN cord-022235-6ircruag 208 20 exclusively exclusively RB cord-022235-6ircruag 208 21 in in IN cord-022235-6ircruag 208 22 the the DT cord-022235-6ircruag 208 23 Golgi Golgi NNP cord-022235-6ircruag 208 24 ( ( -LRB- cord-022235-6ircruag 208 25 418,786 418,786 CD cord-022235-6ircruag 208 26 ) ) -RRB- cord-022235-6ircruag 208 27 . . . cord-022235-6ircruag 209 1 Acceptor Acceptor NNP cord-022235-6ircruag 209 2 octapeptides octapeptide NNS cord-022235-6ircruag 209 3 can can MD cord-022235-6ircruag 209 4 be be VB cord-022235-6ircruag 209 5 O o NN cord-022235-6ircruag 209 6 - - HYPH cord-022235-6ircruag 209 7 glycosylated glycosylated JJ cord-022235-6ircruag 209 8 . . . cord-022235-6ircruag 210 1 7V 7V NNP cord-022235-6ircruag 210 2 - - HYPH cord-022235-6ircruag 210 3 acetylgalactosamine acetylgalactosamine NNP cord-022235-6ircruag 210 4 is be VBZ cord-022235-6ircruag 210 5 the the DT cord-022235-6ircruag 210 6 primary primary JJ cord-022235-6ircruag 210 7 sugar sugar NN cord-022235-6ircruag 210 8 in in IN cord-022235-6ircruag 210 9 O o NN cord-022235-6ircruag 210 10 - - HYPH cord-022235-6ircruag 210 11 linked link VBN cord-022235-6ircruag 210 12 oligosaccharides oligosaccharide NNS cord-022235-6ircruag 210 13 ; ; : cord-022235-6ircruag 210 14 further further JJ cord-022235-6ircruag 210 15 resi resi JJ cord-022235-6ircruag 210 16 dues due NNS cord-022235-6ircruag 210 17 of of IN cord-022235-6ircruag 210 18 galactose galactose NN cord-022235-6ircruag 210 19 , , , cord-022235-6ircruag 210 20 sialic sialic NN cord-022235-6ircruag 210 21 acid acid NN cord-022235-6ircruag 210 22 , , , cord-022235-6ircruag 210 23 fucose fucose NNP cord-022235-6ircruag 210 24 , , , cord-022235-6ircruag 210 25 ^-acetylgalactosamine ^-acetylgalactosamine ADD cord-022235-6ircruag 210 26 , , , cord-022235-6ircruag 210 27 and and CC cord-022235-6ircruag 210 28 Nacetylglucosamine Nacetylglucosamine NNP cord-022235-6ircruag 210 29 can can MD cord-022235-6ircruag 210 30 then then RB cord-022235-6ircruag 210 31 be be VB cord-022235-6ircruag 210 32 added add VBN cord-022235-6ircruag 210 33 . . . cord-022235-6ircruag 211 1 Lipid lipid NN cord-022235-6ircruag 211 2 intermediates intermediate NNS cord-022235-6ircruag 211 3 are be VBP cord-022235-6ircruag 211 4 not not RB cord-022235-6ircruag 211 5 in in IN cord-022235-6ircruag 211 6 volved volved NN cord-022235-6ircruag 211 7 in in IN cord-022235-6ircruag 211 8 O o NN cord-022235-6ircruag 211 9 - - HYPH cord-022235-6ircruag 211 10 glycosylation glycosylation NN cord-022235-6ircruag 211 11 , , , cord-022235-6ircruag 211 12 and and CC cord-022235-6ircruag 211 13 the the DT cord-022235-6ircruag 211 14 reaction reaction NN cord-022235-6ircruag 211 15 is be VBZ cord-022235-6ircruag 211 16 not not RB cord-022235-6ircruag 211 17 inhibited inhibit VBN cord-022235-6ircruag 211 18 by by IN cord-022235-6ircruag 211 19 tunicamy tunicamy NNP cord-022235-6ircruag 211 20 cin cin NNP cord-022235-6ircruag 211 21 ( ( -LRB- cord-022235-6ircruag 211 22 418 418 CD cord-022235-6ircruag 211 23 ) ) -RRB- cord-022235-6ircruag 211 24 , , , cord-022235-6ircruag 211 25 but but CC cord-022235-6ircruag 211 26 it -PRON- PRP cord-022235-6ircruag 211 27 is be VBZ cord-022235-6ircruag 211 28 not not RB cord-022235-6ircruag 211 29 known know VBN cord-022235-6ircruag 211 30 whether whether IN cord-022235-6ircruag 211 31 other other JJ cord-022235-6ircruag 211 32 sugar sugar NN cord-022235-6ircruag 211 33 transferases transferase NNS cord-022235-6ircruag 211 34 are be VBP cord-022235-6ircruag 211 35 in in IN cord-022235-6ircruag 211 36 volved volved NN cord-022235-6ircruag 211 37 in in IN cord-022235-6ircruag 211 38 both both CC cord-022235-6ircruag 211 39 N N NNP cord-022235-6ircruag 211 40 - - : cord-022235-6ircruag 211 41 and and CC cord-022235-6ircruag 211 42 O o NN cord-022235-6ircruag 211 43 - - HYPH cord-022235-6ircruag 211 44 glycosylation glycosylation NN cord-022235-6ircruag 211 45 . . . cord-022235-6ircruag 212 1 Glycosylated Glycosylated NNP cord-022235-6ircruag 212 2 and and CC cord-022235-6ircruag 212 3 unglycosylated unglycosylate VBN cord-022235-6ircruag 212 4 secretory secretory JJ cord-022235-6ircruag 212 5 proteins protein NNS cord-022235-6ircruag 212 6 are be VBP cord-022235-6ircruag 212 7 major major JJ cord-022235-6ircruag 212 8 substrates substrate NNS cord-022235-6ircruag 212 9 for for IN cord-022235-6ircruag 212 10 tyrosine tyrosine NN cord-022235-6ircruag 212 11 sulfation sulfation NN cord-022235-6ircruag 212 12 ( ( -LRB- cord-022235-6ircruag 212 13 501 501 CD cord-022235-6ircruag 212 14 ) ) -RRB- cord-022235-6ircruag 212 15 . . . cord-022235-6ircruag 213 1 Tyrosynylprotein Tyrosynylprotein NNP cord-022235-6ircruag 213 2 sulfotransferase sulfotransferase NNP cord-022235-6ircruag 213 3 is be VBZ cord-022235-6ircruag 213 4 enriched enrich VBN cord-022235-6ircruag 213 5 in in IN cord-022235-6ircruag 213 6 Golgi golgi NN cord-022235-6ircruag 213 7 - - HYPH cord-022235-6ircruag 213 8 derived derive VBN cord-022235-6ircruag 213 9 membrane membrane NN cord-022235-6ircruag 213 10 fractions fraction NNS cord-022235-6ircruag 213 11 and and CC cord-022235-6ircruag 213 12 has have VBZ cord-022235-6ircruag 213 13 its -PRON- PRP$ cord-022235-6ircruag 213 14 active active JJ cord-022235-6ircruag 213 15 site site NN cord-022235-6ircruag 213 16 oriented orient VBN cord-022235-6ircruag 213 17 to to TO cord-022235-6ircruag 213 18 ward ward VB cord-022235-6ircruag 213 19 the the DT cord-022235-6ircruag 213 20 Golgi Golgi NNP cord-022235-6ircruag 213 21 lumen lumen NN cord-022235-6ircruag 213 22 ( ( -LRB- cord-022235-6ircruag 213 23 619 619 CD cord-022235-6ircruag 213 24 ) ) -RRB- cord-022235-6ircruag 213 25 . . . cord-022235-6ircruag 214 1 The the DT cord-022235-6ircruag 214 2 sulfate sulfate NN cord-022235-6ircruag 214 3 donor donor NN cord-022235-6ircruag 214 4 is be VBZ cord-022235-6ircruag 214 5 3 3 CD cord-022235-6ircruag 214 6 ' ' POS cord-022235-6ircruag 214 7 -phosphoadenosine -phosphoadenosine JJ cord-022235-6ircruag 214 8 5 5 CD cord-022235-6ircruag 214 9 phosphosulfate phosphosulfate NN cord-022235-6ircruag 214 10 . . . cord-022235-6ircruag 215 1 Almost almost RB cord-022235-6ircruag 215 2 all all DT cord-022235-6ircruag 215 3 tyrosinated tyrosinate VBN cord-022235-6ircruag 215 4 proteins protein NNS cord-022235-6ircruag 215 5 have have VBP cord-022235-6ircruag 215 6 an an DT cord-022235-6ircruag 215 7 acidic acidic JJ cord-022235-6ircruag 215 8 residue residue NN cord-022235-6ircruag 215 9 , , , cord-022235-6ircruag 215 10 a a DT cord-022235-6ircruag 215 11 glycine glycine NN cord-022235-6ircruag 215 12 or or CC cord-022235-6ircruag 215 13 proline proline NN cord-022235-6ircruag 215 14 residue residue NN cord-022235-6ircruag 215 15 , , , cord-022235-6ircruag 215 16 and and CC cord-022235-6ircruag 215 17 no no DT cord-022235-6ircruag 215 18 cysteines cysteine NNS cord-022235-6ircruag 215 19 , , , cord-022235-6ircruag 215 20 basic basic JJ cord-022235-6ircruag 215 21 residues residue NNS cord-022235-6ircruag 215 22 , , , cord-022235-6ircruag 215 23 extended extended JJ cord-022235-6ircruag 215 24 secondary secondary JJ cord-022235-6ircruag 215 25 structure structure NN cord-022235-6ircruag 215 26 , , , cord-022235-6ircruag 215 27 or or CC cord-022235-6ircruag 215 28 N n CD cord-022235-6ircruag 215 29 - - HYPH cord-022235-6ircruag 215 30 glycosylation glycosylation NN cord-022235-6ircruag 215 31 sites site NNS cord-022235-6ircruag 215 32 close close JJ cord-022235-6ircruag 215 33 to to IN cord-022235-6ircruag 215 34 the the DT cord-022235-6ircruag 215 35 modified modify VBN cord-022235-6ircruag 215 36 tyro tyro NN cord-022235-6ircruag 215 37 sine sine NN cord-022235-6ircruag 215 38 , , , cord-022235-6ircruag 215 39 but but CC cord-022235-6ircruag 215 40 there there EX cord-022235-6ircruag 215 41 does do VBZ cord-022235-6ircruag 215 42 not not RB cord-022235-6ircruag 215 43 appear appear VB cord-022235-6ircruag 215 44 to to TO cord-022235-6ircruag 215 45 be be VB cord-022235-6ircruag 215 46 a a DT cord-022235-6ircruag 215 47 strict strict JJ cord-022235-6ircruag 215 48 consensus consensus NN cord-022235-6ircruag 215 49 sequence sequence NN cord-022235-6ircruag 215 50 around around IN cord-022235-6ircruag 215 51 the the DT cord-022235-6ircruag 215 52 modification modification NN cord-022235-6ircruag 215 53 site site NN cord-022235-6ircruag 215 54 ( ( -LRB- cord-022235-6ircruag 215 55 474,619 474,619 CD cord-022235-6ircruag 215 56 ) ) -RRB- cord-022235-6ircruag 215 57 . . . cord-022235-6ircruag 216 1 Inhibition inhibition NN cord-022235-6ircruag 216 2 of of IN cord-022235-6ircruag 216 3 tyrosine tyrosine NN cord-022235-6ircruag 216 4 sulfation sulfation NN cord-022235-6ircruag 216 5 is be VBZ cord-022235-6ircruag 216 6 reported report VBN cord-022235-6ircruag 216 7 to to TO cord-022235-6ircruag 216 8 retard retard VB cord-022235-6ircruag 216 9 the the DT cord-022235-6ircruag 216 10 exit exit NN cord-022235-6ircruag 216 11 of of IN cord-022235-6ircruag 216 12 a a DT cord-022235-6ircruag 216 13 secretary secretary NN cord-022235-6ircruag 216 14 protein protein NN cord-022235-6ircruag 216 15 from from IN cord-022235-6ircruag 216 16 the the DT cord-022235-6ircruag 216 17 TGN TGN NNP cord-022235-6ircruag 216 18 ( ( -LRB- cord-022235-6ircruag 216 19 331b 331b CD cord-022235-6ircruag 216 20 ) ) -RRB- cord-022235-6ircruag 216 21 . . . cord-022235-6ircruag 217 1 Many many JJ cord-022235-6ircruag 217 2 secretory secretory NN cord-022235-6ircruag 217 3 proteins protein NNS cord-022235-6ircruag 217 4 undergo undergo VBP cord-022235-6ircruag 217 5 secondary secondary JJ cord-022235-6ircruag 217 6 proteolytic proteolytic JJ cord-022235-6ircruag 217 7 processing processing NN cord-022235-6ircruag 217 8 fol fol NN cord-022235-6ircruag 217 9 lowing low VBG cord-022235-6ircruag 217 10 removal removal NN cord-022235-6ircruag 217 11 of of IN cord-022235-6ircruag 217 12 the the DT cord-022235-6ircruag 217 13 signal signal NN cord-022235-6ircruag 217 14 peptide peptide NN cord-022235-6ircruag 217 15 . . . cord-022235-6ircruag 218 1 The the DT cord-022235-6ircruag 218 2 best well RBS cord-022235-6ircruag 218 3 characterized characterize VBN cord-022235-6ircruag 218 4 examples example NNS cord-022235-6ircruag 218 5 of of IN cord-022235-6ircruag 218 6 this this DT cord-022235-6ircruag 218 7 class class NN cord-022235-6ircruag 218 8 of of IN cord-022235-6ircruag 218 9 processed process VBN cord-022235-6ircruag 218 10 secretory secretory JJ cord-022235-6ircruag 218 11 proteins protein NNS cord-022235-6ircruag 218 12 are be VBP cord-022235-6ircruag 218 13 α α NN cord-022235-6ircruag 218 14 factor factor NN cord-022235-6ircruag 218 15 and and CC cord-022235-6ircruag 218 16 killer killer NN cord-022235-6ircruag 218 17 toxin toxin NN cord-022235-6ircruag 218 18 of of IN cord-022235-6ircruag 218 19 yeasts yeast NNS cord-022235-6ircruag 218 20 . . . cord-022235-6ircruag 219 1 The the DT cord-022235-6ircruag 219 2 α α NN cord-022235-6ircruag 219 3 - - HYPH cord-022235-6ircruag 219 4 factor factor NN cord-022235-6ircruag 219 5 peptide peptide NN cord-022235-6ircruag 219 6 is be VBZ cord-022235-6ircruag 219 7 present present JJ cord-022235-6ircruag 219 8 four four CD cord-022235-6ircruag 219 9 times time NNS cord-022235-6ircruag 219 10 in in IN cord-022235-6ircruag 219 11 the the DT cord-022235-6ircruag 219 12 pro pro JJ cord-022235-6ircruag 219 13 - - JJ cord-022235-6ircruag 219 14 a a DT cord-022235-6ircruag 219 15 - - HYPH cord-022235-6ircruag 219 16 factor factor NN cord-022235-6ircruag 219 17 protein protein NN cord-022235-6ircruag 219 18 which which WDT cord-022235-6ircruag 219 19 reaches reach VBZ cord-022235-6ircruag 219 20 the the DT cord-022235-6ircruag 219 21 Golgi Golgi NNP cord-022235-6ircruag 219 22 apparatus apparatus NN cord-022235-6ircruag 219 23 . . . cord-022235-6ircruag 220 1 This this DT cord-022235-6ircruag 220 2 probably probably RB cord-022235-6ircruag 220 3 represents represent VBZ cord-022235-6ircruag 220 4 a a DT cord-022235-6ircruag 220 5 way way NN cord-022235-6ircruag 220 6 of of IN cord-022235-6ircruag 220 7 reducing reduce VBG cord-022235-6ircruag 220 8 " " `` cord-022235-6ircruag 220 9 shipping shipping NN cord-022235-6ircruag 220 10 costs cost NNS cord-022235-6ircruag 220 11 " " '' cord-022235-6ircruag 220 12 since since IN cord-022235-6ircruag 220 13 α α NN cord-022235-6ircruag 220 14 factor factor NN cord-022235-6ircruag 220 15 itself -PRON- PRP cord-022235-6ircruag 220 16 may may MD cord-022235-6ircruag 220 17 be be VB cord-022235-6ircruag 220 18 too too RB cord-022235-6ircruag 220 19 small small JJ cord-022235-6ircruag 220 20 to to TO cord-022235-6ircruag 220 21 be be VB cord-022235-6ircruag 220 22 efficiently efficiently RB cord-022235-6ircruag 220 23 transported transport VBN cord-022235-6ircruag 220 24 to to IN cord-022235-6ircruag 220 25 the the DT cord-022235-6ircruag 220 26 RERM RERM NNP cord-022235-6ircruag 220 27 and and CC cord-022235-6ircruag 220 28 it -PRON- PRP cord-022235-6ircruag 220 29 would would MD cord-022235-6ircruag 220 30 be be VB cord-022235-6ircruag 220 31 inefficient inefficient JJ cord-022235-6ircruag 220 32 to to TO cord-022235-6ircruag 220 33 pad pad VB cord-022235-6ircruag 220 34 the the DT cord-022235-6ircruag 220 35 polypeptide polypeptide NN cord-022235-6ircruag 220 36 out out RP cord-022235-6ircruag 220 37 with with IN cord-022235-6ircruag 220 38 redundant redundant JJ cord-022235-6ircruag 220 39 sequences sequence NNS cord-022235-6ircruag 220 40 ( ( -LRB- cord-022235-6ircruag 220 41 602 602 CD cord-022235-6ircruag 220 42 ) ) -RRB- cord-022235-6ircruag 220 43 . . . cord-022235-6ircruag 221 1 Julius Julius NNP cord-022235-6ircruag 221 2 et et NNP cord-022235-6ircruag 221 3 al al NNP cord-022235-6ircruag 221 4 . . . cord-022235-6ircruag 222 1 ( ( -LRB- cord-022235-6ircruag 222 2 545 545 CD cord-022235-6ircruag 222 3 ) ) -RRB- cord-022235-6ircruag 222 4 found find VBD cord-022235-6ircruag 222 5 that that IN cord-022235-6ircruag 222 6 pro pro JJ cord-022235-6ircruag 222 7 - - JJ cord-022235-6ircruag 222 8 a a DT cord-022235-6ircruag 222 9 - - HYPH cord-022235-6ircruag 222 10 factor factor NN cord-022235-6ircruag 222 11 processing processing NN cord-022235-6ircruag 222 12 was be VBD cord-022235-6ircruag 222 13 blocked block VBN cord-022235-6ircruag 222 14 by by IN cord-022235-6ircruag 222 15 sec sec NNP cord-022235-6ircruag 222 16 mutations mutation NNS cord-022235-6ircruag 222 17 , , , cord-022235-6ircruag 222 18 which which WDT cord-022235-6ircruag 222 19 prevented prevent VBD cord-022235-6ircruag 222 20 protein protein NN cord-022235-6ircruag 222 21 movement movement NN cord-022235-6ircruag 222 22 through through IN cord-022235-6ircruag 222 23 the the DT cord-022235-6ircruag 222 24 Golgi Golgi NNP cord-022235-6ircruag 222 25 , , , cord-022235-6ircruag 222 26 and and CC cord-022235-6ircruag 222 27 that that IN cord-022235-6ircruag 222 28 mature mature NN cord-022235-6ircruag 222 29 α α NN cord-022235-6ircruag 222 30 factor factor NN cord-022235-6ircruag 222 31 was be VBD cord-022235-6ircruag 222 32 normally normally RB cord-022235-6ircruag 222 33 present present JJ cord-022235-6ircruag 222 34 in in IN cord-022235-6ircruag 222 35 secretory secretory JJ cord-022235-6ircruag 222 36 vesicles vesicle NNS cord-022235-6ircruag 222 37 en en IN cord-022235-6ircruag 222 38 route route NN cord-022235-6ircruag 222 39 to to IN cord-022235-6ircruag 222 40 the the DT cord-022235-6ircruag 222 41 cell cell NN cord-022235-6ircruag 222 42 surface surface NN cord-022235-6ircruag 222 43 . . . cord-022235-6ircruag 223 1 Thus thus RB cord-022235-6ircruag 223 2 , , , cord-022235-6ircruag 223 3 processing processing NN cord-022235-6ircruag 223 4 occurs occur VBZ cord-022235-6ircruag 223 5 in in IN cord-022235-6ircruag 223 6 the the DT cord-022235-6ircruag 223 7 Golgi Golgi NNP cord-022235-6ircruag 223 8 . . . cord-022235-6ircruag 224 1 Some some DT cord-022235-6ircruag 224 2 viral viral JJ cord-022235-6ircruag 224 3 coat coat NN cord-022235-6ircruag 224 4 proteins protein NNS cord-022235-6ircruag 224 5 such such JJ cord-022235-6ircruag 224 6 as as IN cord-022235-6ircruag 224 7 influenza influenza NN cord-022235-6ircruag 224 8 virus virus NN cord-022235-6ircruag 224 9 HA HA NNP cord-022235-6ircruag 224 10 are be VBP cord-022235-6ircruag 224 11 also also RB cord-022235-6ircruag 224 12 proteolytically proteolytically RB cord-022235-6ircruag 224 13 processed process VBN cord-022235-6ircruag 224 14 in in IN cord-022235-6ircruag 224 15 the the DT cord-022235-6ircruag 224 16 trans trans NNP cord-022235-6ircruag 224 17 - - NNP cord-022235-6ircruag 224 18 Golgi Golgi NNP cord-022235-6ircruag 224 19 network network NN cord-022235-6ircruag 224 20 ( ( -LRB- cord-022235-6ircruag 224 21 TGN TGN NNP cord-022235-6ircruag 224 22 ) ) -RRB- cord-022235-6ircruag 224 23 or or CC cord-022235-6ircruag 224 24 trans trans NNP cord-022235-6ircruag 224 25 Golgi Golgi NNP cord-022235-6ircruag 224 26 . . . cord-022235-6ircruag 225 1 Processing processing NN cord-022235-6ircruag 225 2 of of IN cord-022235-6ircruag 225 3 mammalian mammalian JJ cord-022235-6ircruag 225 4 prohormones prohormone NNS cord-022235-6ircruag 225 5 , , , cord-022235-6ircruag 225 6 which which WDT cord-022235-6ircruag 225 7 is be VBZ cord-022235-6ircruag 225 8 similar similar JJ cord-022235-6ircruag 225 9 to to IN cord-022235-6ircruag 225 10 that that DT cord-022235-6ircruag 225 11 of of IN cord-022235-6ircruag 225 12 pro pro JJ cord-022235-6ircruag 225 13 - - JJ cord-022235-6ircruag 225 14 α α JJ cord-022235-6ircruag 225 15 factor factor NN cord-022235-6ircruag 225 16 , , , cord-022235-6ircruag 225 17 occurs occur VBZ cord-022235-6ircruag 225 18 in in IN cord-022235-6ircruag 225 19 secretory secretory JJ cord-022235-6ircruag 225 20 granules granule NNS cord-022235-6ircruag 225 21 budding bud VBG cord-022235-6ircruag 225 22 from from IN cord-022235-6ircruag 225 23 the the DT cord-022235-6ircruag 225 24 trans trans NNP cord-022235-6ircruag 225 25 Golgi Golgi NNP cord-022235-6ircruag 225 26 [ [ -LRB- cord-022235-6ircruag 225 27 see see UH cord-022235-6ircruag 225 28 Section section NN cord-022235-6ircruag 225 29 V.G.4.C v.g.4.c NN cord-022235-6ircruag 225 30 and and CC cord-022235-6ircruag 225 31 ( ( -LRB- cord-022235-6ircruag 225 32 1003 1003 CD cord-022235-6ircruag 225 33 ) ) -RRB- cord-022235-6ircruag 225 34 ] ] -RRB- cord-022235-6ircruag 225 35 ) ) -RRB- cord-022235-6ircruag 225 36 . . . cord-022235-6ircruag 226 1 The the DT cord-022235-6ircruag 226 2 four four CD cord-022235-6ircruag 226 3 13-residue 13-residue CD cord-022235-6ircruag 226 4 - - HYPH cord-022235-6ircruag 226 5 long long NN cord-022235-6ircruag 226 6 α α NN cord-022235-6ircruag 226 7 - - HYPH cord-022235-6ircruag 226 8 factor factor NN cord-022235-6ircruag 226 9 peptides peptide NNS cord-022235-6ircruag 226 10 in in IN cord-022235-6ircruag 226 11 pro pro JJ cord-022235-6ircruag 226 12 - - JJ cord-022235-6ircruag 226 13 α α JJ cord-022235-6ircruag 226 14 factor factor NN cord-022235-6ircruag 226 15 were be VBD cord-022235-6ircruag 226 16 found find VBN cord-022235-6ircruag 226 17 to to TO cord-022235-6ircruag 226 18 be be VB cord-022235-6ircruag 226 19 separated separate VBN cord-022235-6ircruag 226 20 by by IN cord-022235-6ircruag 226 21 6 6 CD cord-022235-6ircruag 226 22 - - SYM cord-022235-6ircruag 226 23 8 8 CD cord-022235-6ircruag 226 24 residues residue NNS cord-022235-6ircruag 226 25 ( ( -LRB- cord-022235-6ircruag 226 26 Lys Lys NNP cord-022235-6ircruag 226 27 - - HYPH cord-022235-6ircruag 226 28 Arg Arg NNP cord-022235-6ircruag 226 29 - - HYPH cord-022235-6ircruag 226 30 Glu Glu NNP cord-022235-6ircruag 226 31 - - HYPH cord-022235-6ircruag 226 32 Ala Ala NNP cord-022235-6ircruag 226 33 - - HYPH cord-022235-6ircruag 226 34 Asp Asp NNP cord-022235-6ircruag 226 35 - - HYPH cord-022235-6ircruag 226 36 Ala Ala NNP cord-022235-6ircruag 226 37 - - HYPH cord-022235-6ircruag 226 38 Glu Glu NNP cord-022235-6ircruag 226 39 - - HYPH cord-022235-6ircruag 226 40 Asp Asp NNP cord-022235-6ircruag 226 41 ) ) -RRB- cord-022235-6ircruag 226 42 ( ( -LRB- cord-022235-6ircruag 226 43 602 602 CD cord-022235-6ircruag 226 44 ) ) -RRB- cord-022235-6ircruag 226 45 . . . cord-022235-6ircruag 227 1 This this DT cord-022235-6ircruag 227 2 suggests suggest VBZ cord-022235-6ircruag 227 3 that that IN cord-022235-6ircruag 227 4 at at RB cord-022235-6ircruag 227 5 least least RBS cord-022235-6ircruag 227 6 one one CD cord-022235-6ircruag 227 7 processing processing NN cord-022235-6ircruag 227 8 protease protease NN cord-022235-6ircruag 227 9 has have VBZ cord-022235-6ircruag 227 10 trypsin trypsin NN cord-022235-6ircruag 227 11 - - HYPH cord-022235-6ircruag 227 12 or or CC cord-022235-6ircruag 227 13 chymotrypsin chymotrypsin JJ cord-022235-6ircruag 227 14 - - HYPH cord-022235-6ircruag 227 15 like like JJ cord-022235-6ircruag 227 16 activity activity NN cord-022235-6ircruag 227 17 . . . cord-022235-6ircruag 228 1 Killer killer NN cord-022235-6ircruag 228 2 toxins toxin NNS cord-022235-6ircruag 228 3 are be VBP cord-022235-6ircruag 228 4 processed process VBN cord-022235-6ircruag 228 5 at at IN cord-022235-6ircruag 228 6 similar similar JJ cord-022235-6ircruag 228 7 sites site NNS cord-022235-6ircruag 228 8 ( ( -LRB- cord-022235-6ircruag 228 9 245,1074 245,1074 NNP cord-022235-6ircruag 228 10 ) ) -RRB- cord-022235-6ircruag 228 11 . . . cord-022235-6ircruag 229 1 In in IN cord-022235-6ircruag 229 2 fact fact NN cord-022235-6ircruag 229 3 , , , cord-022235-6ircruag 229 4 the the DT cord-022235-6ircruag 229 5 enzyme enzyme NN cord-022235-6ircruag 229 6 which which WDT cord-022235-6ircruag 229 7 performs perform VBZ cord-022235-6ircruag 229 8 this this DT cord-022235-6ircruag 229 9 initial initial JJ cord-022235-6ircruag 229 10 processing processing NN cord-022235-6ircruag 229 11 step step NN cord-022235-6ircruag 229 12 , , , cord-022235-6ircruag 229 13 the the DT cord-022235-6ircruag 229 14 product product NN cord-022235-6ircruag 229 15 of of IN cord-022235-6ircruag 229 16 the the DT cord-022235-6ircruag 229 17 KEX2 KEX2 NNP cord-022235-6ircruag 229 18 gene gene NN cord-022235-6ircruag 229 19 , , , cord-022235-6ircruag 229 20 is be VBZ cord-022235-6ircruag 229 21 a a DT cord-022235-6ircruag 229 22 Ca Ca NNP cord-022235-6ircruag 229 23 2 2 CD cord-022235-6ircruag 229 24 + + CD cord-022235-6ircruag 229 25 -thiol -thiol : cord-022235-6ircruag 229 26 protease protease NN cord-022235-6ircruag 229 27 which which WDT cord-022235-6ircruag 229 28 cleaves cleave VBZ cord-022235-6ircruag 229 29 between between IN cord-022235-6ircruag 229 30 basic basic JJ cord-022235-6ircruag 229 31 residues residue NNS cord-022235-6ircruag 229 32 . . . cord-022235-6ircruag 230 1 The the DT cord-022235-6ircruag 230 2 enzyme enzyme NN cord-022235-6ircruag 230 3 is be VBZ cord-022235-6ircruag 230 4 inhibited inhibit VBN cord-022235-6ircruag 230 5 by by IN cord-022235-6ircruag 230 6 anti anti JJ cord-022235-6ircruag 230 7 - - JJ cord-022235-6ircruag 230 8 αΐ-trypsin αΐ-trypsin JJ cord-022235-6ircruag 230 9 and and CC cord-022235-6ircruag 230 10 can can MD cord-022235-6ircruag 230 11 correctly correctly RB cord-022235-6ircruag 230 12 process process VB cord-022235-6ircruag 230 13 mammalian mammalian JJ cord-022235-6ircruag 230 14 proalbumin proalbumin NNP cord-022235-6ircruag 230 15 ( ( -LRB- cord-022235-6ircruag 230 16 53 53 CD cord-022235-6ircruag 230 17 ) ) -RRB- cord-022235-6ircruag 230 18 . . . cord-022235-6ircruag 231 1 Strains strain NNS cord-022235-6ircruag 231 2 mutated mutate VBN cord-022235-6ircruag 231 3 at at IN cord-022235-6ircruag 232 1 KEX2 KEX2 NNP cord-022235-6ircruag 232 2 secrete secrete VB cord-022235-6ircruag 232 3 unprocessed unprocessed JJ cord-022235-6ircruag 232 4 pro pro JJ cord-022235-6ircruag 232 5 - - JJ cord-022235-6ircruag 232 6 α α JJ cord-022235-6ircruag 232 7 factor factor NN cord-022235-6ircruag 232 8 ( ( -LRB- cord-022235-6ircruag 232 9 546 546 CD cord-022235-6ircruag 232 10 ) ) -RRB- cord-022235-6ircruag 232 11 . . . cord-022235-6ircruag 233 1 The the DT cord-022235-6ircruag 233 2 product product NN cord-022235-6ircruag 233 3 of of IN cord-022235-6ircruag 233 4 the the DT cord-022235-6ircruag 233 5 STE13 STE13 NNP cord-022235-6ircruag 233 6 gene gene NN cord-022235-6ircruag 233 7 , , , cord-022235-6ircruag 233 8 a a DT cord-022235-6ircruag 233 9 membrane membrane NN cord-022235-6ircruag 233 10 - - HYPH cord-022235-6ircruag 233 11 associated associate VBN cord-022235-6ircruag 233 12 aminopeptidase aminopeptidase NN cord-022235-6ircruag 233 13 ( ( -LRB- cord-022235-6ircruag 233 14 544 544 CD cord-022235-6ircruag 233 15 ) ) -RRB- cord-022235-6ircruag 233 16 , , , cord-022235-6ircruag 233 17 processes process VBZ cord-022235-6ircruag 233 18 the the DT cord-022235-6ircruag 233 19 Nterminal Nterminal NNP cord-022235-6ircruag 233 20 tetrapeptide tetrapeptide NN cord-022235-6ircruag 233 21 , , , cord-022235-6ircruag 233 22 and and CC cord-022235-6ircruag 233 23 further further JJ cord-022235-6ircruag 233 24 processing processing NN cord-022235-6ircruag 233 25 of of IN cord-022235-6ircruag 233 26 the the DT cord-022235-6ircruag 233 27 C C NNP cord-022235-6ircruag 233 28 - - HYPH cord-022235-6ircruag 233 29 terminus terminus NN cord-022235-6ircruag 233 30 is be VBZ cord-022235-6ircruag 233 31 per per IN cord-022235-6ircruag 233 32 formed form VBN cord-022235-6ircruag 233 33 by by IN cord-022235-6ircruag 233 34 KEX1 KEX1 NNP cord-022235-6ircruag 233 35 -encoded -encoded JJ cord-022235-6ircruag 233 36 carboxypeptidase carboxypeptidase NN cord-022235-6ircruag 233 37 ( ( -LRB- cord-022235-6ircruag 233 38 245 245 CD cord-022235-6ircruag 233 39 ) ) -RRB- cord-022235-6ircruag 233 40 . . . cord-022235-6ircruag 234 1 Other other JJ cord-022235-6ircruag 234 2 secretory secretory JJ cord-022235-6ircruag 234 3 pro pro FW cord-022235-6ircruag 234 4 teins tein NNS cord-022235-6ircruag 234 5 produced produce VBN cord-022235-6ircruag 234 6 by by IN cord-022235-6ircruag 234 7 different different JJ cord-022235-6ircruag 234 8 species specie NNS cord-022235-6ircruag 234 9 of of IN cord-022235-6ircruag 234 10 yeasts yeast NNS cord-022235-6ircruag 234 11 may may MD cord-022235-6ircruag 234 12 also also RB cord-022235-6ircruag 234 13 be be VB cord-022235-6ircruag 234 14 processed process VBN cord-022235-6ircruag 234 15 by by IN cord-022235-6ircruag 234 16 one one CD cord-022235-6ircruag 234 17 or or CC cord-022235-6ircruag 234 18 more more JJR cord-022235-6ircruag 234 19 of of IN cord-022235-6ircruag 234 20 these these DT cord-022235-6ircruag 234 21 enzymes enzyme NNS cord-022235-6ircruag 234 22 ( ( -LRB- cord-022235-6ircruag 234 23 695b 695b NNP cord-022235-6ircruag 234 24 ) ) -RRB- cord-022235-6ircruag 234 25 . . . cord-022235-6ircruag 235 1 Results result NNS cord-022235-6ircruag 235 2 from from IN cord-022235-6ircruag 235 3 a a DT cord-022235-6ircruag 235 4 number number NN cord-022235-6ircruag 235 5 of of IN cord-022235-6ircruag 235 6 experimental experimental JJ cord-022235-6ircruag 235 7 approaches approach NNS cord-022235-6ircruag 235 8 ( ( -LRB- cord-022235-6ircruag 235 9 summarized summarize VBN cord-022235-6ircruag 235 10 below below IN cord-022235-6ircruag 235 11 ) ) -RRB- cord-022235-6ircruag 235 12 show show VBP cord-022235-6ircruag 235 13 quite quite RB cord-022235-6ircruag 235 14 conclusively conclusively RB cord-022235-6ircruag 235 15 that that IN cord-022235-6ircruag 235 16 individual individual JJ cord-022235-6ircruag 235 17 Golgi Golgi NNP cord-022235-6ircruag 235 18 cisternae cisternae NNP cord-022235-6ircruag 235 19 are be VBP cord-022235-6ircruag 235 20 separate separate JJ cord-022235-6ircruag 235 21 , , , cord-022235-6ircruag 235 22 bio bio NNP cord-022235-6ircruag 235 23 chemically chemically RB cord-022235-6ircruag 235 24 and and CC cord-022235-6ircruag 235 25 functionally functionally RB cord-022235-6ircruag 235 26 distinct distinct JJ cord-022235-6ircruag 235 27 entities entity NNS cord-022235-6ircruag 235 28 organized organize VBN cord-022235-6ircruag 235 29 according accord VBG cord-022235-6ircruag 235 30 to to IN cord-022235-6ircruag 235 31 a a DT cord-022235-6ircruag 235 32 very very RB cord-022235-6ircruag 235 33 strict strict JJ cord-022235-6ircruag 235 34 pattern pattern NN cord-022235-6ircruag 235 35 and and CC cord-022235-6ircruag 235 36 that that IN cord-022235-6ircruag 235 37 secretory secretory JJ cord-022235-6ircruag 235 38 proteins protein NNS cord-022235-6ircruag 235 39 progress progress VBP cord-022235-6ircruag 235 40 in in IN cord-022235-6ircruag 235 41 a a DT cord-022235-6ircruag 235 42 synchronized synchronized JJ cord-022235-6ircruag 235 43 wave wave NN cord-022235-6ircruag 235 44 from from IN cord-022235-6ircruag 235 45 one one CD cord-022235-6ircruag 235 46 end end NN cord-022235-6ircruag 235 47 of of IN cord-022235-6ircruag 235 48 the the DT cord-022235-6ircruag 235 49 stack stack NN cord-022235-6ircruag 235 50 of of IN cord-022235-6ircruag 235 51 cisternae cisternae NN cord-022235-6ircruag 235 52 to to IN cord-022235-6ircruag 235 53 the the DT cord-022235-6ircruag 235 54 other other JJ cord-022235-6ircruag 235 55 en en IN cord-022235-6ircruag 235 56 route route NN cord-022235-6ircruag 235 57 to to IN cord-022235-6ircruag 235 58 their -PRON- PRP$ cord-022235-6ircruag 235 59 final final JJ cord-022235-6ircruag 235 60 destinations destination NNS cord-022235-6ircruag 235 61 . . . cord-022235-6ircruag 236 1 According accord VBG cord-022235-6ircruag 236 2 to to IN cord-022235-6ircruag 236 3 these these DT cord-022235-6ircruag 236 4 data datum NNS cord-022235-6ircruag 236 5 , , , cord-022235-6ircruag 236 6 Golgi Golgi NNP cord-022235-6ircruag 236 7 stacks stack NNS cord-022235-6ircruag 236 8 must must MD cord-022235-6ircruag 236 9 contain contain VB cord-022235-6ircruag 236 10 at at RB cord-022235-6ircruag 236 11 least least RBS cord-022235-6ircruag 236 12 three three CD cord-022235-6ircruag 236 13 distinct distinct JJ cord-022235-6ircruag 236 14 cisternae cisternae NN cord-022235-6ircruag 236 15 ( ( -LRB- cord-022235-6ircruag 236 16 generally generally RB cord-022235-6ircruag 236 17 referred refer VBN cord-022235-6ircruag 236 18 to to IN cord-022235-6ircruag 236 19 as as IN cord-022235-6ircruag 236 20 cis cis NNP cord-022235-6ircruag 236 21 , , , cord-022235-6ircruag 236 22 medial medial NNP cord-022235-6ircruag 236 23 , , , cord-022235-6ircruag 236 24 and and CC cord-022235-6ircruag 236 25 trans trans NNP cord-022235-6ircruag 236 26 according accord VBG cord-022235-6ircruag 236 27 to to IN cord-022235-6ircruag 236 28 their -PRON- PRP$ cord-022235-6ircruag 236 29 orientation orientation NN cord-022235-6ircruag 236 30 with with IN cord-022235-6ircruag 236 31 respect respect NN cord-022235-6ircruag 236 32 to to IN cord-022235-6ircruag 236 33 the the DT cord-022235-6ircruag 236 34 ER ER NNP cord-022235-6ircruag 236 35 ) ) -RRB- cord-022235-6ircruag 236 36 . . . cord-022235-6ircruag 237 1 Heterogeneity heterogeneity NN cord-022235-6ircruag 237 2 within within IN cord-022235-6ircruag 237 3 these these DT cord-022235-6ircruag 237 4 " " `` cord-022235-6ircruag 237 5 domains domain NNS cord-022235-6ircruag 237 6 " " '' cord-022235-6ircruag 237 7 indicates indicate VBZ cord-022235-6ircruag 237 8 that that IN cord-022235-6ircruag 237 9 the the DT cord-022235-6ircruag 237 10 actual actual JJ cord-022235-6ircruag 237 11 number number NN cord-022235-6ircruag 237 12 of of IN cord-022235-6ircruag 237 13 cisternae cisternae NNP cord-022235-6ircruag 237 14 is be VBZ cord-022235-6ircruag 237 15 probably probably RB cord-022235-6ircruag 237 16 higher high JJR cord-022235-6ircruag 237 17 than than IN cord-022235-6ircruag 237 18 three three CD cord-022235-6ircruag 237 19 ( ( -LRB- cord-022235-6ircruag 237 20 see see VB cord-022235-6ircruag 237 21 Table table NN cord-022235-6ircruag 237 22 V v NN cord-022235-6ircruag 237 23 .l .l NNP cord-022235-6ircruag 237 24 , , , cord-022235-6ircruag 237 25 Fig Fig NNP cord-022235-6ircruag 237 26 . . . cord-022235-6ircruag 238 1 V v NN cord-022235-6ircruag 238 2 .3 .3 CD cord-022235-6ircruag 238 3 ) ) -RRB- cord-022235-6ircruag 239 1 ( ( -LRB- cord-022235-6ircruag 239 2 299 299 CD cord-022235-6ircruag 239 3 ) ) -RRB- cord-022235-6ircruag 239 4 . . . cord-022235-6ircruag 240 1 Cells cell NNS cord-022235-6ircruag 240 2 normally normally RB cord-022235-6ircruag 240 3 have have VBP cord-022235-6ircruag 240 4 one one CD cord-022235-6ircruag 240 5 or or CC cord-022235-6ircruag 240 6 a a DT cord-022235-6ircruag 240 7 very very RB cord-022235-6ircruag 240 8 limited limited JJ cord-022235-6ircruag 240 9 number number NN cord-022235-6ircruag 240 10 of of IN cord-022235-6ircruag 240 11 Golgi Golgi NNP cord-022235-6ircruag 240 12 stacks stack NNS cord-022235-6ircruag 240 13 . . . cord-022235-6ircruag 241 1 The the DT cord-022235-6ircruag 241 2 stacks stack NNS cord-022235-6ircruag 241 3 break break VBP cord-022235-6ircruag 241 4 up up RP cord-022235-6ircruag 241 5 into into IN cord-022235-6ircruag 241 6 clusters cluster NNS cord-022235-6ircruag 241 7 of of IN cord-022235-6ircruag 241 8 many many JJ cord-022235-6ircruag 241 9 vesicles vesicle NNS cord-022235-6ircruag 241 10 during during IN cord-022235-6ircruag 241 11 mitosis mitosis NN cord-022235-6ircruag 241 12 , , , cord-022235-6ircruag 241 13 apparently apparently RB cord-022235-6ircruag 241 14 to to TO cord-022235-6ircruag 241 15 ensure ensure VB cord-022235-6ircruag 241 16 equal equal JJ cord-022235-6ircruag 241 17 partitioning partitioning NN cord-022235-6ircruag 241 18 of of IN cord-022235-6ircruag 241 19 Golgi Golgi NNP cord-022235-6ircruag 241 20 components component NNS cord-022235-6ircruag 241 21 to to IN cord-022235-6ircruag 241 22 daughter daughter NN cord-022235-6ircruag 241 23 cells cell NNS cord-022235-6ircruag 241 24 , , , cord-022235-6ircruag 241 25 although although IN cord-022235-6ircruag 241 26 the the DT cord-022235-6ircruag 241 27 number number NN cord-022235-6ircruag 241 28 of of IN cord-022235-6ircruag 241 29 Golgi Golgi NNP cord-022235-6ircruag 241 30 clusters cluster NNS cord-022235-6ircruag 241 31 produced produce VBN cord-022235-6ircruag 241 32 is be VBZ cord-022235-6ircruag 241 33 far far RB cord-022235-6ircruag 241 34 in in IN cord-022235-6ircruag 241 35 excess excess NN cord-022235-6ircruag 241 36 of of IN cord-022235-6ircruag 241 37 that that DT cord-022235-6ircruag 241 38 required require VBN cord-022235-6ircruag 241 39 for for IN cord-022235-6ircruag 241 40 this this DT cord-022235-6ircruag 241 41 pur pur NN cord-022235-6ircruag 241 42 pose pose NNP cord-022235-6ircruag 241 43 . . . cord-022235-6ircruag 242 1 Golgi golgi NN cord-022235-6ircruag 242 2 breakdown breakdown NN cord-022235-6ircruag 242 3 presumably presumably RB cord-022235-6ircruag 242 4 involves involve VBZ cord-022235-6ircruag 242 5 membrane membrane NN cord-022235-6ircruag 242 6 fission fission NN cord-022235-6ircruag 242 7 , , , cord-022235-6ircruag 242 8 so so IN cord-022235-6ircruag 242 9 each each DT cord-022235-6ircruag 242 10 cluster cluster NN cord-022235-6ircruag 242 11 of of IN cord-022235-6ircruag 242 12 vesicles vesicle NNS cord-022235-6ircruag 242 13 contains contain VBZ cord-022235-6ircruag 242 14 cis cis NNP cord-022235-6ircruag 242 15 , , , cord-022235-6ircruag 242 16 medial medial JJ cord-022235-6ircruag 242 17 , , , cord-022235-6ircruag 242 18 and and CC cord-022235-6ircruag 242 19 trans trans NNP cord-022235-6ircruag 242 20 components component NNS cord-022235-6ircruag 242 21 ( ( -LRB- cord-022235-6ircruag 242 22 662 662 CD cord-022235-6ircruag 242 23 ) ) -RRB- cord-022235-6ircruag 242 24 . . . cord-022235-6ircruag 243 1 Pro Pro NNP cord-022235-6ircruag 243 2 tein tein NNP cord-022235-6ircruag 243 3 secretion secretion NN cord-022235-6ircruag 243 4 is be VBZ cord-022235-6ircruag 243 5 usually usually RB cord-022235-6ircruag 243 6 shut shut VBN cord-022235-6ircruag 243 7 down down RP cord-022235-6ircruag 243 8 during during IN cord-022235-6ircruag 243 9 mitosis mitosis NN cord-022235-6ircruag 243 10 or or CC cord-022235-6ircruag 243 11 meiosis meiosis NN cord-022235-6ircruag 243 12 , , , cord-022235-6ircruag 243 13 but but CC cord-022235-6ircruag 243 14 the the DT cord-022235-6ircruag 243 15 yeast yeast NN cord-022235-6ircruag 243 16 S. S. NNP cord-022235-6ircruag 243 17 cerevisiae cerevisiae NN cord-022235-6ircruag 243 18 continues continue VBZ cord-022235-6ircruag 243 19 to to TO cord-022235-6ircruag 243 20 process process VB cord-022235-6ircruag 243 21 and and CC cord-022235-6ircruag 243 22 secrete secrete VB cord-022235-6ircruag 243 23 invertase invertase NN cord-022235-6ircruag 243 24 during during IN cord-022235-6ircruag 243 25 mitosis mitosis NN cord-022235-6ircruag 243 26 , , , cord-022235-6ircruag 243 27 implying imply VBG cord-022235-6ircruag 243 28 that that IN cord-022235-6ircruag 243 29 the the DT cord-022235-6ircruag 243 30 Golgi Golgi NNP cord-022235-6ircruag 243 31 fragments fragment NNS cord-022235-6ircruag 243 32 remain remain VBP cord-022235-6ircruag 243 33 active active JJ cord-022235-6ircruag 243 34 ( ( -LRB- cord-022235-6ircruag 243 35 685 685 CD cord-022235-6ircruag 243 36 ) ) -RRB- cord-022235-6ircruag 243 37 . . . cord-022235-6ircruag 244 1 In in IN cord-022235-6ircruag 244 2 general general JJ cord-022235-6ircruag 244 3 , , , cord-022235-6ircruag 244 4 results result NNS cord-022235-6ircruag 244 5 from from IN cord-022235-6ircruag 244 6 different different JJ cord-022235-6ircruag 244 7 analyses analysis NNS cord-022235-6ircruag 244 8 give give VBP cord-022235-6ircruag 244 9 a a DT cord-022235-6ircruag 244 10 coherent coherent JJ cord-022235-6ircruag 244 11 picture picture NN cord-022235-6ircruag 244 12 of of IN cord-022235-6ircruag 244 13 the the DT cord-022235-6ircruag 244 14 cis cis NNP cord-022235-6ircruag 244 15 to to IN cord-022235-6ircruag 244 16 trans trans NNP cord-022235-6ircruag 244 17 organization organization NN cord-022235-6ircruag 244 18 of of IN cord-022235-6ircruag 244 19 Golgi Golgi NNP cord-022235-6ircruag 244 20 cisternae cisternae NNP cord-022235-6ircruag 244 21 ( ( -LRB- cord-022235-6ircruag 244 22 see see VB cord-022235-6ircruag 244 23 Table Table NNP cord-022235-6ircruag 244 24 V v NN cord-022235-6ircruag 244 25 . . NNP cord-022235-6ircruag 244 26 1 1 CD cord-022235-6ircruag 244 27 ) ) -RRB- cord-022235-6ircruag 244 28 , , , cord-022235-6ircruag 244 29 on on IN cord-022235-6ircruag 244 30 the the DT cord-022235-6ircruag 244 31 basis basis NN cord-022235-6ircruag 244 32 of of IN cord-022235-6ircruag 244 33 which which WDT cord-022235-6ircruag 244 34 a a DT cord-022235-6ircruag 244 35 simple simple JJ cord-022235-6ircruag 244 36 map map NN cord-022235-6ircruag 244 37 can can MD cord-022235-6ircruag 244 38 be be VB cord-022235-6ircruag 244 39 drawn draw VBN cord-022235-6ircruag 244 40 ( ( -LRB- cord-022235-6ircruag 244 41 Fig fig NN cord-022235-6ircruag 244 42 . . . cord-022235-6ircruag 244 43 V.3 V.3 NNP cord-022235-6ircruag 244 44 ) ) -RRB- cord-022235-6ircruag 244 45 . . . cord-022235-6ircruag 245 1 The the DT cord-022235-6ircruag 245 2 segregation segregation NN cord-022235-6ircruag 245 3 of of IN cord-022235-6ircruag 245 4 modify modify VB cord-022235-6ircruag 245 5 ing e VBG cord-022235-6ircruag 245 6 enzymes enzyme NNS cord-022235-6ircruag 245 7 presumably presumably RB cord-022235-6ircruag 245 8 allows allow VBZ cord-022235-6ircruag 245 9 prosthetic prosthetic JJ cord-022235-6ircruag 245 10 groups group NNS cord-022235-6ircruag 245 11 to to TO cord-022235-6ircruag 245 12 be be VB cord-022235-6ircruag 245 13 added add VBN cord-022235-6ircruag 245 14 or or CC cord-022235-6ircruag 245 15 removed remove VBN cord-022235-6ircruag 245 16 according accord VBG cord-022235-6ircruag 245 17 to to IN cord-022235-6ircruag 245 18 a a DT cord-022235-6ircruag 245 19 strict strict JJ cord-022235-6ircruag 245 20 sequence sequence NN cord-022235-6ircruag 245 21 and and CC cord-022235-6ircruag 245 22 prevents prevent VBZ cord-022235-6ircruag 245 23 competition competition NN cord-022235-6ircruag 245 24 between between IN cord-022235-6ircruag 245 25 process process NN cord-022235-6ircruag 245 26 ing e VBG cord-022235-6ircruag 245 27 enzymes enzyme NNS cord-022235-6ircruag 245 28 which which WDT cord-022235-6ircruag 245 29 could could MD cord-022235-6ircruag 245 30 act act VB cord-022235-6ircruag 245 31 on on IN cord-022235-6ircruag 245 32 the the DT cord-022235-6ircruag 245 33 same same JJ cord-022235-6ircruag 245 34 substrate substrate NN cord-022235-6ircruag 245 35 ( ( -LRB- cord-022235-6ircruag 245 36 266 266 CD cord-022235-6ircruag 245 37 ) ) -RRB- cord-022235-6ircruag 245 38 . . . cord-022235-6ircruag 246 1 Table table NN cord-022235-6ircruag 246 2 V V NNP cord-022235-6ircruag 246 3 .l .l NN cord-022235-6ircruag 246 4 is be VBZ cord-022235-6ircruag 246 5 five five CD cord-022235-6ircruag 246 6 , , , cord-022235-6ircruag 246 7 but but CC cord-022235-6ircruag 246 8 the the DT cord-022235-6ircruag 246 9 actual actual JJ cord-022235-6ircruag 246 10 number number NN cord-022235-6ircruag 246 11 of of IN cord-022235-6ircruag 246 12 Golgi Golgi NNP cord-022235-6ircruag 246 13 cisternae cisternae NNP cord-022235-6ircruag 246 14 may may MD cord-022235-6ircruag 246 15 be be VB cord-022235-6ircruag 246 16 higher high JJR cord-022235-6ircruag 246 17 than than IN cord-022235-6ircruag 246 18 that that DT cord-022235-6ircruag 246 19 shown show VBN cord-022235-6ircruag 246 20 . . . cord-022235-6ircruag 247 1 NAGn NAGn NNP cord-022235-6ircruag 247 2 , , , cord-022235-6ircruag 247 3 iV iV NNP cord-022235-6ircruag 247 4 - - HYPH cord-022235-6ircruag 247 5 acetylglucosamine acetylglucosamine NNP cord-022235-6ircruag 247 6 ; ; : cord-022235-6ircruag 247 7 TGN TGN NNP cord-022235-6ircruag 247 8 , , , cord-022235-6ircruag 247 9 trans trans NNP cord-022235-6ircruag 247 10 - - NNP cord-022235-6ircruag 247 11 Golgi Golgi NNP cord-022235-6ircruag 247 12 network network NN cord-022235-6ircruag 247 13 . . . cord-022235-6ircruag 248 1 Early early JJ cord-022235-6ircruag 248 2 studies study NNS cord-022235-6ircruag 248 3 showed show VBD cord-022235-6ircruag 248 4 that that IN cord-022235-6ircruag 248 5 one one CD cord-022235-6ircruag 248 6 or or CC cord-022235-6ircruag 248 7 two two CD cord-022235-6ircruag 248 8 cis ci NNS cord-022235-6ircruag 248 9 ( ( -LRB- cord-022235-6ircruag 248 10 ER er NN cord-022235-6ircruag 248 11 - - HYPH cord-022235-6ircruag 248 12 proximal proximal JJ cord-022235-6ircruag 248 13 ) ) -RRB- cord-022235-6ircruag 248 14 Golgi Golgi NNP cord-022235-6ircruag 248 15 cisternae cisternae NNP cord-022235-6ircruag 248 16 were be VBD cord-022235-6ircruag 248 17 preferentially preferentially RB cord-022235-6ircruag 248 18 stained stain VBN cord-022235-6ircruag 248 19 during during IN cord-022235-6ircruag 248 20 prolonged prolong VBN cord-022235-6ircruag 248 21 exposure exposure NN cord-022235-6ircruag 248 22 to to IN cord-022235-6ircruag 248 23 osmium osmium NN cord-022235-6ircruag 248 24 [ [ -LRB- cord-022235-6ircruag 248 25 pre pre NNP cord-022235-6ircruag 248 26 sumably sumably RB cord-022235-6ircruag 248 27 due due JJ cord-022235-6ircruag 248 28 to to IN cord-022235-6ircruag 248 29 strong strong JJ cord-022235-6ircruag 248 30 reducing reduce VBG cord-022235-6ircruag 248 31 conditions condition NNS cord-022235-6ircruag 248 32 in in IN cord-022235-6ircruag 248 33 these these DT cord-022235-6ircruag 248 34 cisternae cisternae NN cord-022235-6ircruag 248 35 ( ( -LRB- cord-022235-6ircruag 248 36 333 333 CD cord-022235-6ircruag 248 37 ) ) -RRB- cord-022235-6ircruag 248 38 ] ] -RRB- cord-022235-6ircruag 248 39 . . . cord-022235-6ircruag 249 1 Sub sub JJ cord-022235-6ircruag 249 2 sequent sequent JJ cord-022235-6ircruag 249 3 histological histological NN cord-022235-6ircruag 249 4 and and CC cord-022235-6ircruag 249 5 cytochemical cytochemical JJ cord-022235-6ircruag 249 6 tests test NNS cord-022235-6ircruag 249 7 showed show VBD cord-022235-6ircruag 249 8 that that IN cord-022235-6ircruag 249 9 many many JJ cord-022235-6ircruag 249 10 enzymes enzyme NNS cord-022235-6ircruag 249 11 involved involve VBN cord-022235-6ircruag 249 12 in in IN cord-022235-6ircruag 249 13 protein protein NN cord-022235-6ircruag 249 14 glycosylation glycosylation NN cord-022235-6ircruag 249 15 and and CC cord-022235-6ircruag 249 16 other other JJ cord-022235-6ircruag 249 17 reactions reaction NNS cord-022235-6ircruag 249 18 , , , cord-022235-6ircruag 249 19 as as RB cord-022235-6ircruag 249 20 well well RB cord-022235-6ircruag 249 21 as as IN cord-022235-6ircruag 249 22 some some DT cord-022235-6ircruag 249 23 proteins protein NNS cord-022235-6ircruag 249 24 with with IN cord-022235-6ircruag 249 25 unknown unknown JJ cord-022235-6ircruag 249 26 function function NN cord-022235-6ircruag 249 27 , , , cord-022235-6ircruag 249 28 were be VBD cord-022235-6ircruag 249 29 not not RB cord-022235-6ircruag 249 30 evenly evenly RB cord-022235-6ircruag 249 31 distributed distribute VBN cord-022235-6ircruag 249 32 through through IN cord-022235-6ircruag 249 33 the the DT cord-022235-6ircruag 249 34 Golgi Golgi NNP cord-022235-6ircruag 249 35 stock stock NN cord-022235-6ircruag 249 36 but but CC cord-022235-6ircruag 249 37 were be VBD cord-022235-6ircruag 249 38 compartmentalized compartmentalize VBN cord-022235-6ircruag 249 39 in in IN cord-022235-6ircruag 249 40 one one CD cord-022235-6ircruag 249 41 or or CC cord-022235-6ircruag 249 42 two two CD cord-022235-6ircruag 249 43 cisternae cisternae JJ cord-022235-6ircruag 249 44 ( ( -LRB- cord-022235-6ircruag 249 45 Table table NN cord-022235-6ircruag 249 46 _SP cord-022235-6ircruag 249 47 V v NN cord-022235-6ircruag 249 48 .l .l NNP cord-022235-6ircruag 249 49 ) ) -RRB- cord-022235-6ircruag 249 50 . . . cord-022235-6ircruag 250 1 The the DT cord-022235-6ircruag 250 2 techniques technique NNS cord-022235-6ircruag 250 3 employed employ VBN cord-022235-6ircruag 250 4 include include VBP cord-022235-6ircruag 250 5 immunocytological immunocytological NNP cord-022235-6ircruag 250 6 detection detection NN cord-022235-6ircruag 250 7 of of IN cord-022235-6ircruag 250 8 proteins protein NNS cord-022235-6ircruag 250 9 using use VBG cord-022235-6ircruag 250 10 specific specific JJ cord-022235-6ircruag 250 11 antibodies antibody NNS cord-022235-6ircruag 250 12 , , , cord-022235-6ircruag 250 13 enzymatic enzymatic JJ cord-022235-6ircruag 250 14 cytochemical cytochemical JJ cord-022235-6ircruag 250 15 reagents reagent NNS cord-022235-6ircruag 250 16 for for IN cord-022235-6ircruag 250 17 specific specific JJ cord-022235-6ircruag 250 18 enzymes enzyme NNS cord-022235-6ircruag 250 19 , , , cord-022235-6ircruag 250 20 and and CC cord-022235-6ircruag 250 21 the the DT cord-022235-6ircruag 250 22 detection detection NN cord-022235-6ircruag 250 23 of of IN cord-022235-6ircruag 250 24 lectin lectin NN cord-022235-6ircruag 250 25 - - HYPH cord-022235-6ircruag 250 26 specific specific JJ cord-022235-6ircruag 250 27 sugar sugar NN cord-022235-6ircruag 250 28 residues residue NNS cord-022235-6ircruag 250 29 on on IN cord-022235-6ircruag 250 30 terminally terminally RB cord-022235-6ircruag 250 31 modified modify VBN cord-022235-6ircruag 250 32 glycoproteins glycoprotein NNS cord-022235-6ircruag 250 33 in in IN cord-022235-6ircruag 250 34 transit transit NN cord-022235-6ircruag 250 35 through through IN cord-022235-6ircruag 250 36 the the DT cord-022235-6ircruag 250 37 Golgi Golgi NNP cord-022235-6ircruag 250 38 ( ( -LRB- cord-022235-6ircruag 250 39 Table table NN cord-022235-6ircruag 250 40 V v NN cord-022235-6ircruag 250 41 .l .l NNP cord-022235-6ircruag 250 42 ) ) -RRB- cord-022235-6ircruag 250 43 . . . cord-022235-6ircruag 251 1 As as IN cord-022235-6ircruag 251 2 noted note VBN cord-022235-6ircruag 251 3 by by IN cord-022235-6ircruag 251 4 Farquhar Farquhar NNP cord-022235-6ircruag 251 5 ( ( -LRB- cord-022235-6ircruag 251 6 299 299 CD cord-022235-6ircruag 251 7 ) ) -RRB- cord-022235-6ircruag 251 8 , , , cord-022235-6ircruag 251 9 some some DT cord-022235-6ircruag 251 10 studies study NNS cord-022235-6ircruag 251 11 with with IN cord-022235-6ircruag 251 12 different different JJ cord-022235-6ircruag 251 13 cell cell NN cord-022235-6ircruag 251 14 lines line NNS cord-022235-6ircruag 251 15 give give VBP cord-022235-6ircruag 251 16 conflicting conflicting JJ cord-022235-6ircruag 251 17 results result NNS cord-022235-6ircruag 251 18 , , , cord-022235-6ircruag 251 19 suggesting suggest VBG cord-022235-6ircruag 251 20 that that IN cord-022235-6ircruag 251 21 the the DT cord-022235-6ircruag 251 22 organization organization NN cord-022235-6ircruag 251 23 of of IN cord-022235-6ircruag 251 24 the the DT cord-022235-6ircruag 251 25 Golgi Golgi NNP cord-022235-6ircruag 251 26 stack stack NN cord-022235-6ircruag 251 27 may may MD cord-022235-6ircruag 251 28 vary vary VB cord-022235-6ircruag 251 29 depending depend VBG cord-022235-6ircruag 251 30 on on IN cord-022235-6ircruag 251 31 cell cell NN cord-022235-6ircruag 251 32 type type NN cord-022235-6ircruag 251 33 and and CC cord-022235-6ircruag 251 34 function function NN cord-022235-6ircruag 251 35 . . . cord-022235-6ircruag 252 1 Intermediates intermediate NNS cord-022235-6ircruag 252 2 in in IN cord-022235-6ircruag 252 3 the the DT cord-022235-6ircruag 252 4 secretion secretion NN cord-022235-6ircruag 252 5 pathway pathway NN cord-022235-6ircruag 252 6 can can MD cord-022235-6ircruag 252 7 be be VB cord-022235-6ircruag 252 8 detected detect VBN cord-022235-6ircruag 252 9 by by IN cord-022235-6ircruag 252 10 pulse pulse NN cord-022235-6ircruag 252 11 - - HYPH cord-022235-6ircruag 252 12 chase chase NN cord-022235-6ircruag 252 13 experiments experiment NNS cord-022235-6ircruag 252 14 in in IN cord-022235-6ircruag 252 15 which which WDT cord-022235-6ircruag 252 16 oligosaccharides oligosaccharide NNS cord-022235-6ircruag 252 17 or or CC cord-022235-6ircruag 252 18 other other JJ cord-022235-6ircruag 252 19 prosthetic prosthetic JJ cord-022235-6ircruag 252 20 groups group NNS cord-022235-6ircruag 252 21 are be VBP cord-022235-6ircruag 252 22 la la RB cord-022235-6ircruag 252 23 beled bele VBN cord-022235-6ircruag 252 24 by by IN cord-022235-6ircruag 252 25 the the DT cord-022235-6ircruag 252 26 metabolic metabolic JJ cord-022235-6ircruag 252 27 incorporation incorporation NN cord-022235-6ircruag 252 28 of of IN cord-022235-6ircruag 252 29 radioactive radioactive JJ cord-022235-6ircruag 252 30 precursors precursor NNS cord-022235-6ircruag 252 31 . . . cord-022235-6ircruag 253 1 The the DT cord-022235-6ircruag 253 2 se se FW cord-022235-6ircruag 253 3 quence quence NN cord-022235-6ircruag 253 4 in in IN cord-022235-6ircruag 253 5 which which WDT cord-022235-6ircruag 253 6 processing processing NN cord-022235-6ircruag 253 7 occurs occur VBZ cord-022235-6ircruag 253 8 can can MD cord-022235-6ircruag 253 9 thus thus RB cord-022235-6ircruag 253 10 be be VB cord-022235-6ircruag 253 11 determined determine VBN cord-022235-6ircruag 253 12 and and CC cord-022235-6ircruag 253 13 correlated correlate VBN cord-022235-6ircruag 253 14 with with IN cord-022235-6ircruag 253 15 the the DT cord-022235-6ircruag 253 16 location location NN cord-022235-6ircruag 253 17 of of IN cord-022235-6ircruag 253 18 processing processing NN cord-022235-6ircruag 253 19 enzymes enzyme NNS cord-022235-6ircruag 253 20 in in IN cord-022235-6ircruag 253 21 the the DT cord-022235-6ircruag 253 22 Golgi Golgi NNP cord-022235-6ircruag 253 23 cisternae cisternae NNP cord-022235-6ircruag 253 24 . . . cord-022235-6ircruag 254 1 Golgi Golgi NNP cord-022235-6ircruag 254 2 enzymes enzyme NNS cord-022235-6ircruag 254 3 involved involve VBN cord-022235-6ircruag 254 4 in in IN cord-022235-6ircruag 254 5 N n CD cord-022235-6ircruag 254 6 - - : cord-022235-6ircruag 254 7 and and CC cord-022235-6ircruag 254 8 O o NN cord-022235-6ircruag 254 9 - - HYPH cord-022235-6ircruag 254 10 glycosylation glycosylation NN cord-022235-6ircruag 254 11 , , , cord-022235-6ircruag 254 12 sulfation sulfation NN cord-022235-6ircruag 254 13 , , , cord-022235-6ircruag 254 14 and and CC cord-022235-6ircruag 254 15 palmi palmi NNP cord-022235-6ircruag 254 16 toylation toylation NNP cord-022235-6ircruag 254 17 of of IN cord-022235-6ircruag 254 18 secretory secretory JJ cord-022235-6ircruag 254 19 proteins protein NNS cord-022235-6ircruag 254 20 can can MD cord-022235-6ircruag 254 21 be be VB cord-022235-6ircruag 254 22 separated separate VBN cord-022235-6ircruag 254 23 by by IN cord-022235-6ircruag 254 24 density density NN cord-022235-6ircruag 254 25 gradient gradient NN cord-022235-6ircruag 254 26 cen cen NNP cord-022235-6ircruag 254 27 trifugation trifugation NN cord-022235-6ircruag 254 28 of of IN cord-022235-6ircruag 254 29 Golgi Golgi NNP cord-022235-6ircruag 254 30 - - HYPH cord-022235-6ircruag 254 31 derived derive VBN cord-022235-6ircruag 254 32 vesicles vesicle NNS cord-022235-6ircruag 254 33 , , , cord-022235-6ircruag 254 34 which which WDT cord-022235-6ircruag 254 35 apparently apparently RB cord-022235-6ircruag 254 36 differ differ VBP cord-022235-6ircruag 254 37 in in IN cord-022235-6ircruag 254 38 density density NN cord-022235-6ircruag 254 39 due due IN cord-022235-6ircruag 254 40 to to IN cord-022235-6ircruag 254 41 differences difference NNS cord-022235-6ircruag 254 42 in in IN cord-022235-6ircruag 254 43 cardiolipin cardiolipin JJ cord-022235-6ircruag 254 44 content content NN cord-022235-6ircruag 254 45 ( ( -LRB- cord-022235-6ircruag 254 46 817 817 CD cord-022235-6ircruag 254 47 ) ) -RRB- cord-022235-6ircruag 254 48 . . . cord-022235-6ircruag 255 1 Enzymes enzyme NNS cord-022235-6ircruag 255 2 identified identify VBN cord-022235-6ircruag 255 3 in in IN cord-022235-6ircruag 255 4 cis cis NNP cord-022235-6ircruag 255 5 Golgi Golgi NNP cord-022235-6ircruag 255 6 by by IN cord-022235-6ircruag 255 7 kinetic kinetic JJ cord-022235-6ircruag 255 8 and and CC cord-022235-6ircruag 255 9 histological histological JJ cord-022235-6ircruag 255 10 or or CC cord-022235-6ircruag 255 11 cytochemical cytochemical JJ cord-022235-6ircruag 255 12 tests test NNS cord-022235-6ircruag 255 13 are be VBP cord-022235-6ircruag 255 14 generally generally RB cord-022235-6ircruag 255 15 found find VBN cord-022235-6ircruag 255 16 in in IN cord-022235-6ircruag 255 17 heavier heavy JJR cord-022235-6ircruag 255 18 Golgi Golgi NNP cord-022235-6ircruag 255 19 membrane membrane NN cord-022235-6ircruag 255 20 fractions fraction NNS cord-022235-6ircruag 255 21 , , , cord-022235-6ircruag 255 22 and and CC cord-022235-6ircruag 255 23 density density NN cord-022235-6ircruag 255 24 is be VBZ cord-022235-6ircruag 255 25 now now RB cord-022235-6ircruag 255 26 widely widely RB cord-022235-6ircruag 255 27 used use VBN cord-022235-6ircruag 255 28 to to TO cord-022235-6ircruag 255 29 define define VB cord-022235-6ircruag 255 30 the the DT cord-022235-6ircruag 255 31 approximate approximate JJ cord-022235-6ircruag 255 32 location location NN cord-022235-6ircruag 255 33 of of IN cord-022235-6ircruag 255 34 Golgi Golgi NNP cord-022235-6ircruag 255 35 proteins protein NNS cord-022235-6ircruag 255 36 in in IN cord-022235-6ircruag 255 37 the the DT cord-022235-6ircruag 255 38 stack stack NN cord-022235-6ircruag 255 39 ( ( -LRB- cord-022235-6ircruag 255 40 Table table NN cord-022235-6ircruag 255 41 V v NN cord-022235-6ircruag 255 42 .l .l NNP cord-022235-6ircruag 255 43 ) ) -RRB- cord-022235-6ircruag 255 44 . . . cord-022235-6ircruag 256 1 The the DT cord-022235-6ircruag 256 2 ionophore ionophore NNP cord-022235-6ircruag 256 3 monesin monesin NNP cord-022235-6ircruag 256 4 slows slow VBZ cord-022235-6ircruag 256 5 or or CC cord-022235-6ircruag 256 6 arrests arrest VBZ cord-022235-6ircruag 256 7 intra intra JJ cord-022235-6ircruag 256 8 - - JJ cord-022235-6ircruag 256 9 Golgi golgi JJ cord-022235-6ircruag 256 10 transport transport NN cord-022235-6ircruag 256 11 and and CC cord-022235-6ircruag 256 12 inhibits inhibit VBZ cord-022235-6ircruag 256 13 late late RB cord-022235-6ircruag 256 14 ( ( -LRB- cord-022235-6ircruag 256 15 trans trans NNP cord-022235-6ircruag 256 16 ) ) -RRB- cord-022235-6ircruag 256 17 Golgi Golgi NNP cord-022235-6ircruag 256 18 functions function NNS cord-022235-6ircruag 256 19 , , , cord-022235-6ircruag 256 20 and and CC cord-022235-6ircruag 256 21 can can MD cord-022235-6ircruag 256 22 thus thus RB cord-022235-6ircruag 256 23 be be VB cord-022235-6ircruag 256 24 used use VBN cord-022235-6ircruag 256 25 to to TO cord-022235-6ircruag 256 26 distinguish distinguish VB cord-022235-6ircruag 256 27 between between IN cord-022235-6ircruag 256 28 early early JJ cord-022235-6ircruag 256 29 and and CC cord-022235-6ircruag 256 30 late late JJ cord-022235-6ircruag 256 31 Golgi Golgi NNP cord-022235-6ircruag 256 32 processing processing NN cord-022235-6ircruag 256 33 events event NNS cord-022235-6ircruag 256 34 . . . cord-022235-6ircruag 257 1 Monesin Monesin NNP cord-022235-6ircruag 257 2 also also RB cord-022235-6ircruag 257 3 causes cause VBZ cord-022235-6ircruag 257 4 secretory secretory JJ cord-022235-6ircruag 257 5 proteins protein NNS cord-022235-6ircruag 257 6 to to TO cord-022235-6ircruag 257 7 accumulate accumulate VB cord-022235-6ircruag 257 8 in in IN cord-022235-6ircruag 257 9 medial medial JJ cord-022235-6ircruag 257 10 or or CC cord-022235-6ircruag 257 11 late late JJ cord-022235-6ircruag 257 12 Golgi Golgi NNP cord-022235-6ircruag 257 13 compartments compartment NNS cord-022235-6ircruag 257 14 , , , cord-022235-6ircruag 257 15 which which WDT cord-022235-6ircruag 257 16 be be VBP cord-022235-6ircruag 257 17 come come VBN cord-022235-6ircruag 257 18 distended distended JJ cord-022235-6ircruag 257 19 and and CC cord-022235-6ircruag 257 20 vacuolated vacuolated JJ cord-022235-6ircruag 257 21 , , , cord-022235-6ircruag 257 22 aiding aid VBG cord-022235-6ircruag 257 23 their -PRON- PRP$ cord-022235-6ircruag 257 24 identification identification NN cord-022235-6ircruag 257 25 by by IN cord-022235-6ircruag 257 26 cytochemi cytochemi NNP cord-022235-6ircruag 257 27 cal cal NNP cord-022235-6ircruag 257 28 methods methods NNP cord-022235-6ircruag 257 29 ( ( -LRB- cord-022235-6ircruag 257 30 400,905,1108 400,905,1108 CD cord-022235-6ircruag 257 31 ) ) -RRB- cord-022235-6ircruag 257 32 . . . cord-022235-6ircruag 258 1 However however RB cord-022235-6ircruag 258 2 , , , cord-022235-6ircruag 258 3 as as IN cord-022235-6ircruag 258 4 discussed discuss VBN cord-022235-6ircruag 258 5 by by IN cord-022235-6ircruag 258 6 Dunphy Dunphy NNP cord-022235-6ircruag 258 7 and and CC cord-022235-6ircruag 258 8 Rothman Rothman NNP cord-022235-6ircruag 258 9 ( ( -LRB- cord-022235-6ircruag 258 10 266 266 CD cord-022235-6ircruag 258 11 ) ) -RRB- cord-022235-6ircruag 258 12 , , , cord-022235-6ircruag 258 13 studies study NNS cord-022235-6ircruag 258 14 on on IN cord-022235-6ircruag 258 15 the the DT cord-022235-6ircruag 258 16 effects effect NNS cord-022235-6ircruag 258 17 of of IN cord-022235-6ircruag 258 18 monesin monesin NN cord-022235-6ircruag 258 19 in in IN cord-022235-6ircruag 258 20 different different JJ cord-022235-6ircruag 258 21 cell cell NN cord-022235-6ircruag 258 22 types type NNS cord-022235-6ircruag 258 23 often often RB cord-022235-6ircruag 258 24 give give VBP cord-022235-6ircruag 258 25 conflicting conflicting JJ cord-022235-6ircruag 258 26 results result NNS cord-022235-6ircruag 258 27 and and CC cord-022235-6ircruag 258 28 do do VBP cord-022235-6ircruag 258 29 not not RB cord-022235-6ircruag 258 30 necessarily necessarily RB cord-022235-6ircruag 258 31 indicate indicate VB cord-022235-6ircruag 258 32 the the DT cord-022235-6ircruag 258 33 precise precise JJ cord-022235-6ircruag 258 34 site site NN cord-022235-6ircruag 258 35 of of IN cord-022235-6ircruag 258 36 secretory secretory JJ cord-022235-6ircruag 258 37 protein protein NN cord-022235-6ircruag 258 38 accumulation accumulation NN cord-022235-6ircruag 258 39 or or CC cord-022235-6ircruag 258 40 processing processing NN cord-022235-6ircruag 258 41 . . . cord-022235-6ircruag 259 1 Oligosaccharides Oligosaccharides NNP cord-022235-6ircruag 259 2 on on IN cord-022235-6ircruag 259 3 secretory secretory JJ cord-022235-6ircruag 259 4 proteins protein NNS cord-022235-6ircruag 259 5 are be VBP cord-022235-6ircruag 259 6 only only RB cord-022235-6ircruag 259 7 cleaved cleave VBN cord-022235-6ircruag 259 8 by by IN cord-022235-6ircruag 259 9 endoglycosi endoglycosi NNP cord-022235-6ircruag 259 10 dase dase NNP cord-022235-6ircruag 259 11 Η Η NNP cord-022235-6ircruag 259 12 before before IN cord-022235-6ircruag 259 13 they -PRON- PRP cord-022235-6ircruag 259 14 are be VBP cord-022235-6ircruag 259 15 processed process VBN cord-022235-6ircruag 259 16 by by IN cord-022235-6ircruag 259 17 NAGn nagn NN cord-022235-6ircruag 259 18 transferase transferase NNP cord-022235-6ircruag 259 19 I I NNP cord-022235-6ircruag 259 20 and and CC cord-022235-6ircruag 259 21 mannosi mannosi NNP cord-022235-6ircruag 259 22 dase dase NNP cord-022235-6ircruag 259 23 II II NNP cord-022235-6ircruag 259 24 . . . cord-022235-6ircruag 260 1 These these DT cord-022235-6ircruag 260 2 enzymes enzyme NNS cord-022235-6ircruag 260 3 are be VBP cord-022235-6ircruag 260 4 probably probably RB cord-022235-6ircruag 260 5 located locate VBN cord-022235-6ircruag 260 6 in in IN cord-022235-6ircruag 260 7 medial medial JJ cord-022235-6ircruag 260 8 Golgi Golgi NNP cord-022235-6ircruag 260 9 cisternae cisternae NNP cord-022235-6ircruag 260 10 . . . cord-022235-6ircruag 261 1 Thus thus RB cord-022235-6ircruag 261 2 , , , cord-022235-6ircruag 261 3 endoglycosidase endoglycosidase NN cord-022235-6ircruag 261 4 Η η NN cord-022235-6ircruag 261 5 - - HYPH cord-022235-6ircruag 261 6 sensitivity sensitivity NN cord-022235-6ircruag 261 7 provides provide VBZ cord-022235-6ircruag 261 8 a a DT cord-022235-6ircruag 261 9 simple simple JJ cord-022235-6ircruag 261 10 test test NN cord-022235-6ircruag 261 11 for for IN cord-022235-6ircruag 261 12 determin determin VB cord-022235-6ircruag 261 13 ing ing NNP cord-022235-6ircruag 261 14 whether whether IN cord-022235-6ircruag 261 15 secretory secretory JJ cord-022235-6ircruag 261 16 proteins protein NNS cord-022235-6ircruag 261 17 have have VBP cord-022235-6ircruag 261 18 reached reach VBN cord-022235-6ircruag 261 19 these these DT cord-022235-6ircruag 261 20 cisternae cisternae NN cord-022235-6ircruag 261 21 ( ( -LRB- cord-022235-6ircruag 261 22 267 267 CD cord-022235-6ircruag 261 23 ) ) -RRB- cord-022235-6ircruag 261 24 . . . cord-022235-6ircruag 262 1 Rothman Rothman NNP cord-022235-6ircruag 262 2 et et NNP cord-022235-6ircruag 262 3 al al NNP cord-022235-6ircruag 262 4 . . . cord-022235-6ircruag 263 1 ( ( -LRB- cord-022235-6ircruag 263 2 969 969 CD cord-022235-6ircruag 263 3 , , , cord-022235-6ircruag 263 4 970 970 CD cord-022235-6ircruag 263 5 ) ) -RRB- cord-022235-6ircruag 263 6 demonstrated demonstrate VBD cord-022235-6ircruag 263 7 that that IN cord-022235-6ircruag 263 8 pulse pulse NN cord-022235-6ircruag 263 9 - - HYPH cord-022235-6ircruag 263 10 labeled label VBN cord-022235-6ircruag 263 11 VSV VSV NNP cord-022235-6ircruag 263 12 G G NNP cord-022235-6ircruag 263 13 protein protein NN cord-022235-6ircruag 263 14 present present JJ cord-022235-6ircruag 263 15 in in IN cord-022235-6ircruag 263 16 the the DT cord-022235-6ircruag 263 17 Golgi Golgi NNP cord-022235-6ircruag 263 18 of of IN cord-022235-6ircruag 263 19 a a DT cord-022235-6ircruag 263 20 cell cell NN cord-022235-6ircruag 263 21 lacking lack VBG cord-022235-6ircruag 263 22 a a DT cord-022235-6ircruag 263 23 particular particular JJ cord-022235-6ircruag 263 24 modification modification NN cord-022235-6ircruag 263 25 enzyme enzyme NN cord-022235-6ircruag 263 26 can can MD cord-022235-6ircruag 263 27 be be VB cord-022235-6ircruag 263 28 modified modify VBN cord-022235-6ircruag 263 29 upon upon IN cord-022235-6ircruag 263 30 fusion fusion NN cord-022235-6ircruag 263 31 with with IN cord-022235-6ircruag 263 32 a a DT cord-022235-6ircruag 263 33 cell cell NN cord-022235-6ircruag 263 34 producing produce VBG cord-022235-6ircruag 263 35 the the DT cord-022235-6ircruag 263 36 modifying modify VBG cord-022235-6ircruag 263 37 enzyme enzyme NN cord-022235-6ircruag 263 38 . . . cord-022235-6ircruag 264 1 The the DT cord-022235-6ircruag 264 2 Golgi Golgi NNP cord-022235-6ircruag 264 3 cisternae cisternae NN cord-022235-6ircruag 264 4 from from IN cord-022235-6ircruag 264 5 donor donor NN cord-022235-6ircruag 264 6 and and CC cord-022235-6ircruag 264 7 recipient recipient NN cord-022235-6ircruag 264 8 cells cell NNS cord-022235-6ircruag 264 9 did do VBD cord-022235-6ircruag 264 10 not not RB cord-022235-6ircruag 264 11 fuse fuse VB cord-022235-6ircruag 264 12 , , , cord-022235-6ircruag 264 13 and and CC cord-022235-6ircruag 264 14 the the DT cord-022235-6ircruag 264 15 G g NN cord-022235-6ircruag 264 16 protein protein NN cord-022235-6ircruag 264 17 , , , cord-022235-6ircruag 264 18 rather rather RB cord-022235-6ircruag 264 19 than than IN cord-022235-6ircruag 264 20 the the DT cord-022235-6ircruag 264 21 modifying modify VBG cord-022235-6ircruag 264 22 enzyme enzyme NN cord-022235-6ircruag 264 23 , , , cord-022235-6ircruag 264 24 was be VBD cord-022235-6ircruag 264 25 transferred transfer VBN cord-022235-6ircruag 264 26 from from IN cord-022235-6ircruag 264 27 one one CD cord-022235-6ircruag 264 28 cisterna cisterna NNP cord-022235-6ircruag 264 29 to to IN cord-022235-6ircruag 264 30 the the DT cord-022235-6ircruag 264 31 other other JJ cord-022235-6ircruag 264 32 . . . cord-022235-6ircruag 265 1 These these DT cord-022235-6ircruag 265 2 observations observation NNS cord-022235-6ircruag 265 3 have have VBP cord-022235-6ircruag 265 4 implications implication NNS cord-022235-6ircruag 265 5 for for IN cord-022235-6ircruag 265 6 the the DT cord-022235-6ircruag 265 7 mecha mecha NNP cord-022235-6ircruag 265 8 nisms nism NNS cord-022235-6ircruag 265 9 of of IN cord-022235-6ircruag 265 10 protein protein NN cord-022235-6ircruag 265 11 movement movement NN cord-022235-6ircruag 265 12 between between IN cord-022235-6ircruag 265 13 cisternae cisternae NNP cord-022235-6ircruag 265 14 ( ( -LRB- cord-022235-6ircruag 265 15 Section section NN cord-022235-6ircruag 265 16 V.F V.F NNP cord-022235-6ircruag 265 17 ) ) -RRB- cord-022235-6ircruag 265 18 but but CC cord-022235-6ircruag 265 19 , , , cord-022235-6ircruag 265 20 like like IN cord-022235-6ircruag 265 21 in in IN cord-022235-6ircruag 265 22 vivo vivo NN cord-022235-6ircruag 265 23 kinetic kinetic JJ cord-022235-6ircruag 265 24 experiments experiment NNS cord-022235-6ircruag 265 25 , , , cord-022235-6ircruag 265 26 may may MD cord-022235-6ircruag 265 27 also also RB cord-022235-6ircruag 265 28 indicate indicate VB cord-022235-6ircruag 265 29 the the DT cord-022235-6ircruag 265 30 sequence sequence NN cord-022235-6ircruag 265 31 in in IN cord-022235-6ircruag 265 32 which which WDT cord-022235-6ircruag 265 33 pros pro VBZ cord-022235-6ircruag 265 34 thetic thetic JJ cord-022235-6ircruag 265 35 groups group NNS cord-022235-6ircruag 265 36 are be VBP cord-022235-6ircruag 265 37 added add VBN cord-022235-6ircruag 265 38 or or CC cord-022235-6ircruag 265 39 removed remove VBN cord-022235-6ircruag 265 40 ( ( -LRB- cord-022235-6ircruag 265 41 _SP cord-022235-6ircruag 265 42 Having have VBG cord-022235-6ircruag 265 43 established establish VBN cord-022235-6ircruag 265 44 that that IN cord-022235-6ircruag 265 45 secretory secretory JJ cord-022235-6ircruag 265 46 proteins protein NNS cord-022235-6ircruag 265 47 are be VBP cord-022235-6ircruag 265 48 progressively progressively RB cord-022235-6ircruag 265 49 processed process VBN cord-022235-6ircruag 265 50 as as IN cord-022235-6ircruag 265 51 they -PRON- PRP cord-022235-6ircruag 265 52 migrate migrate VBP cord-022235-6ircruag 265 53 through through IN cord-022235-6ircruag 265 54 the the DT cord-022235-6ircruag 265 55 Golgi Golgi NNP cord-022235-6ircruag 265 56 stacks stack NNS cord-022235-6ircruag 265 57 , , , cord-022235-6ircruag 265 58 we -PRON- PRP cord-022235-6ircruag 265 59 now now RB cord-022235-6ircruag 265 60 turn turn VBP cord-022235-6ircruag 265 61 our -PRON- PRP$ cord-022235-6ircruag 265 62 attention attention NN cord-022235-6ircruag 265 63 to to IN cord-022235-6ircruag 265 64 how how WRB cord-022235-6ircruag 265 65 the the DT cord-022235-6ircruag 265 66 proteins protein NNS cord-022235-6ircruag 265 67 themselves -PRON- PRP cord-022235-6ircruag 265 68 migrate migrate VBP cord-022235-6ircruag 265 69 between between IN cord-022235-6ircruag 265 70 cisternae cisternae NNP cord-022235-6ircruag 265 71 , , , cord-022235-6ircruag 265 72 and and CC cord-022235-6ircruag 265 73 how how WRB cord-022235-6ircruag 265 74 Golgi Golgi NNP cord-022235-6ircruag 265 75 pro pro JJ cord-022235-6ircruag 265 76 teins tein NNS cord-022235-6ircruag 265 77 can can MD cord-022235-6ircruag 265 78 be be VB cord-022235-6ircruag 265 79 specifically specifically RB cord-022235-6ircruag 265 80 retained retain VBN cord-022235-6ircruag 265 81 within within IN cord-022235-6ircruag 265 82 specific specific JJ cord-022235-6ircruag 265 83 cisternae cisternae NN cord-022235-6ircruag 265 84 . . . cord-022235-6ircruag 266 1 Of of IN cord-022235-6ircruag 266 2 all all DT cord-022235-6ircruag 266 3 of of IN cord-022235-6ircruag 266 4 the the DT cord-022235-6ircruag 266 5 models model NNS cord-022235-6ircruag 266 6 explaining explain VBG cord-022235-6ircruag 266 7 intra intra JJ cord-022235-6ircruag 266 8 - - JJ cord-022235-6ircruag 266 9 Golgi golgi JJ cord-022235-6ircruag 266 10 movement movement NN cord-022235-6ircruag 266 11 of of IN cord-022235-6ircruag 266 12 secretory secretory JJ cord-022235-6ircruag 266 13 pro pro FW cord-022235-6ircruag 266 14 teins tein NNS cord-022235-6ircruag 266 15 ( ( -LRB- cord-022235-6ircruag 266 16 300 300 CD cord-022235-6ircruag 266 17 ) ) -RRB- cord-022235-6ircruag 266 18 , , , cord-022235-6ircruag 266 19 only only RB cord-022235-6ircruag 266 20 that that IN cord-022235-6ircruag 266 21 invoking invoke VBG cord-022235-6ircruag 266 22 shuttle shuttle NN cord-022235-6ircruag 266 23 vesicles vesicle NNS cord-022235-6ircruag 266 24 fits fit VBZ cord-022235-6ircruag 266 25 the the DT cord-022235-6ircruag 266 26 experimental experimental JJ cord-022235-6ircruag 266 27 data datum NNS cord-022235-6ircruag 266 28 showing show VBG cord-022235-6ircruag 266 29 that that IN cord-022235-6ircruag 266 30 Golgi Golgi NNP cord-022235-6ircruag 266 31 cisternae cisternae NNP cord-022235-6ircruag 266 32 are be VBP cord-022235-6ircruag 266 33 distinct distinct JJ cord-022235-6ircruag 266 34 entities entity NNS cord-022235-6ircruag 266 35 . . . cord-022235-6ircruag 267 1 Numerous numerous JJ cord-022235-6ircruag 267 2 proteincoated proteincoate VBN cord-022235-6ircruag 267 3 vesicles vesicle NNS cord-022235-6ircruag 267 4 are be VBP cord-022235-6ircruag 267 5 produced produce VBN cord-022235-6ircruag 267 6 by by IN cord-022235-6ircruag 267 7 Golgi Golgi NNP cord-022235-6ircruag 267 8 stacks stack NNS cord-022235-6ircruag 267 9 in in IN cord-022235-6ircruag 267 10 vitro vitro FW cord-022235-6ircruag 267 11 under under IN cord-022235-6ircruag 267 12 conditions condition NNS cord-022235-6ircruag 267 13 which which WDT cord-022235-6ircruag 267 14 favor favor VBP cord-022235-6ircruag 267 15 the the DT cord-022235-6ircruag 267 16 intra intra JJ cord-022235-6ircruag 267 17 - - JJ cord-022235-6ircruag 267 18 Golgi golgi JJ cord-022235-6ircruag 267 19 movement movement NN cord-022235-6ircruag 267 20 of of IN cord-022235-6ircruag 267 21 secretory secretory JJ cord-022235-6ircruag 267 22 proteins protein NNS cord-022235-6ircruag 267 23 ( ( -LRB- cord-022235-6ircruag 267 24 see see VB cord-022235-6ircruag 267 25 below below RB cord-022235-6ircruag 267 26 ) ) -RRB- cord-022235-6ircruag 268 1 ( ( -LRB- cord-022235-6ircruag 268 2 40,820 40,820 CD cord-022235-6ircruag 268 3 ) ) -RRB- cord-022235-6ircruag 268 4 . . . cord-022235-6ircruag 269 1 These these DT cord-022235-6ircruag 269 2 uniformly uniformly RB cord-022235-6ircruag 269 3 sized sized JJ cord-022235-6ircruag 269 4 vesicles vesicle NNS cord-022235-6ircruag 269 5 were be VBD cord-022235-6ircruag 269 6 shown show VBN cord-022235-6ircruag 269 7 to to TO cord-022235-6ircruag 269 8 contain contain VB cord-022235-6ircruag 269 9 at at IN cord-022235-6ircruag 269 10 least least RBS cord-022235-6ircruag 269 11 one one CD cord-022235-6ircruag 269 12 secretory secretory JJ cord-022235-6ircruag 269 13 protein protein NN cord-022235-6ircruag 269 14 , , , cord-022235-6ircruag 269 15 VSV VSV NNP cord-022235-6ircruag 269 16 G G NNP cord-022235-6ircruag 269 17 protein protein NN cord-022235-6ircruag 269 18 , , , cord-022235-6ircruag 269 19 indicating indicate VBG cord-022235-6ircruag 269 20 that that IN cord-022235-6ircruag 269 21 they -PRON- PRP cord-022235-6ircruag 269 22 could could MD cord-022235-6ircruag 269 23 be be VB cord-022235-6ircruag 269 24 bona bona FW cord-022235-6ircruag 269 25 fide fide FW cord-022235-6ircruag 269 26 intra intra JJ cord-022235-6ircruag 269 27 - - JJ cord-022235-6ircruag 269 28 Golgi golgi JJ cord-022235-6ircruag 269 29 shuttle shuttle NN cord-022235-6ircruag 269 30 vesicles vesicle NNS cord-022235-6ircruag 269 31 ( ( -LRB- cord-022235-6ircruag 269 32 820 820 CD cord-022235-6ircruag 269 33 ) ) -RRB- cord-022235-6ircruag 269 34 . . . cord-022235-6ircruag 270 1 Similar similar JJ cord-022235-6ircruag 270 2 vesicles vesicle NNS cord-022235-6ircruag 270 3 were be VBD cord-022235-6ircruag 270 4 also also RB cord-022235-6ircruag 270 5 found find VBN cord-022235-6ircruag 270 6 around around IN cord-022235-6ircruag 270 7 the the DT cord-022235-6ircruag 270 8 Golgi Golgi NNP cord-022235-6ircruag 270 9 apparatus apparatus NN cord-022235-6ircruag 270 10 in in IN cord-022235-6ircruag 270 11 situ situ NN cord-022235-6ircruag 270 12 ( ( -LRB- cord-022235-6ircruag 270 13 996 996 CD cord-022235-6ircruag 270 14 , , , cord-022235-6ircruag 270 15 1088 1088 CD cord-022235-6ircruag 270 16 ) ) -RRB- cord-022235-6ircruag 270 17 . . . cord-022235-6ircruag 271 1 Furthermore furthermore RB cord-022235-6ircruag 271 2 , , , cord-022235-6ircruag 271 3 different different JJ cord-022235-6ircruag 271 4 se se FW cord-022235-6ircruag 271 5 cretory cretory NNP cord-022235-6ircruag 271 6 proteins protein NNS cord-022235-6ircruag 271 7 destined destine VBN cord-022235-6ircruag 271 8 to to IN cord-022235-6ircruag 271 9 different different JJ cord-022235-6ircruag 271 10 locations location NNS cord-022235-6ircruag 271 11 and and CC cord-022235-6ircruag 271 12 exported export VBN cord-022235-6ircruag 271 13 or or CC cord-022235-6ircruag 271 14 secreted secrete VBN cord-022235-6ircruag 271 15 at at IN cord-022235-6ircruag 271 16 different different JJ cord-022235-6ircruag 271 17 rates rate NNS cord-022235-6ircruag 271 18 were be VBD cord-022235-6ircruag 271 19 present present JJ cord-022235-6ircruag 271 20 in in IN cord-022235-6ircruag 271 21 the the DT cord-022235-6ircruag 271 22 same same JJ cord-022235-6ircruag 271 23 vesicles vesicle NNS cord-022235-6ircruag 271 24 ( ( -LRB- cord-022235-6ircruag 271 25 1088 1088 CD cord-022235-6ircruag 271 26 ) ) -RRB- cord-022235-6ircruag 271 27 , , , cord-022235-6ircruag 271 28 which which WDT cord-022235-6ircruag 271 29 agrees agree VBZ cord-022235-6ircruag 271 30 with with IN cord-022235-6ircruag 271 31 the the DT cord-022235-6ircruag 271 32 idea idea NN cord-022235-6ircruag 271 33 that that IN cord-022235-6ircruag 271 34 secretory secretory JJ cord-022235-6ircruag 271 35 proteins protein NNS cord-022235-6ircruag 271 36 are be VBP cord-022235-6ircruag 271 37 not not RB cord-022235-6ircruag 271 38 segregated segregate VBN cord-022235-6ircruag 271 39 from from IN cord-022235-6ircruag 271 40 each each DT cord-022235-6ircruag 271 41 other other JJ cord-022235-6ircruag 271 42 during during IN cord-022235-6ircruag 271 43 intermediate intermediate JJ cord-022235-6ircruag 271 44 stages stage NNS cord-022235-6ircruag 271 45 in in IN cord-022235-6ircruag 271 46 the the DT cord-022235-6ircruag 271 47 secretory secretory JJ cord-022235-6ircruag 271 48 pathway pathway NN cord-022235-6ircruag 271 49 . . . cord-022235-6ircruag 272 1 The the DT cord-022235-6ircruag 272 2 protein protein NN cord-022235-6ircruag 272 3 coat coat NN cord-022235-6ircruag 272 4 on on IN cord-022235-6ircruag 272 5 these these DT cord-022235-6ircruag 272 6 vesicles vesicle NNS cord-022235-6ircruag 272 7 is be VBZ cord-022235-6ircruag 272 8 distinct distinct JJ cord-022235-6ircruag 272 9 from from IN cord-022235-6ircruag 272 10 the the DT cord-022235-6ircruag 272 11 clathrin clathrin NN cord-022235-6ircruag 272 12 - - HYPH cord-022235-6ircruag 272 13 containing contain VBG cord-022235-6ircruag 272 14 coats coat NNS cord-022235-6ircruag 272 15 present present JJ cord-022235-6ircruag 272 16 on on IN cord-022235-6ircruag 272 17 endocytic endocytic JJ cord-022235-6ircruag 272 18 and and CC cord-022235-6ircruag 272 19 lysosomal lysosomal JJ cord-022235-6ircruag 272 20 vesicles vesicle NNS cord-022235-6ircruag 272 21 and and CC cord-022235-6ircruag 272 22 on on IN cord-022235-6ircruag 272 23 budding bud VBG cord-022235-6ircruag 272 24 secretory secretory JJ cord-022235-6ircruag 272 25 granules granule NNS cord-022235-6ircruag 272 26 ( ( -LRB- cord-022235-6ircruag 272 27 see see VB cord-022235-6ircruag 272 28 Section section NN cord-022235-6ircruag 272 29 V.G.6 V.G.6 NNP cord-022235-6ircruag 272 30 ) ) -RRB- cord-022235-6ircruag 272 31 . . . cord-022235-6ircruag 273 1 The the DT cord-022235-6ircruag 273 2 only only JJ cord-022235-6ircruag 273 3 regions region NNS cord-022235-6ircruag 273 4 of of IN cord-022235-6ircruag 273 5 the the DT cord-022235-6ircruag 273 6 Golgi Golgi NNP cord-022235-6ircruag 273 7 apparatus apparatus NN cord-022235-6ircruag 273 8 which which WDT cord-022235-6ircruag 273 9 have have VBP cord-022235-6ircruag 273 10 pro pro JJ cord-022235-6ircruag 273 11 tein tein NNP cord-022235-6ircruag 273 12 coats coat NNS cord-022235-6ircruag 273 13 are be VBP cord-022235-6ircruag 273 14 those those DT cord-022235-6ircruag 273 15 from from IN cord-022235-6ircruag 273 16 which which WDT cord-022235-6ircruag 273 17 the the DT cord-022235-6ircruag 273 18 Golgi Golgi NNP cord-022235-6ircruag 273 19 shuttle shuttle NN cord-022235-6ircruag 273 20 vesicles vesicle NNS cord-022235-6ircruag 273 21 bud bud NN cord-022235-6ircruag 273 22 . . . cord-022235-6ircruag 274 1 A a DT cord-022235-6ircruag 274 2 number number NN cord-022235-6ircruag 274 3 of of IN cord-022235-6ircruag 274 4 different different JJ cord-022235-6ircruag 274 5 conditions condition NNS cord-022235-6ircruag 274 6 prevent prevent VBP cord-022235-6ircruag 274 7 intra intra JJ cord-022235-6ircruag 274 8 - - JJ cord-022235-6ircruag 274 9 Golgi golgi JJ cord-022235-6ircruag 274 10 movement movement NN cord-022235-6ircruag 274 11 of of IN cord-022235-6ircruag 274 12 secre secre NNP cord-022235-6ircruag 274 13 tory tory NNP cord-022235-6ircruag 274 14 proteins protein NNS cord-022235-6ircruag 274 15 in in IN cord-022235-6ircruag 274 16 vivo vivo NN cord-022235-6ircruag 274 17 and and CC cord-022235-6ircruag 274 18 in in IN cord-022235-6ircruag 274 19 vitro vitro FW cord-022235-6ircruag 274 20 . . . cord-022235-6ircruag 275 1 The the DT cord-022235-6ircruag 275 2 ionophore ionophore NNP cord-022235-6ircruag 275 3 monesin monesin NNP cord-022235-6ircruag 275 4 ( ( -LRB- cord-022235-6ircruag 275 5 Section section NN cord-022235-6ircruag 275 6 V.E.4 V.E.4 NNP cord-022235-6ircruag 275 7 ) ) -RRB- cord-022235-6ircruag 275 8 probably probably RB cord-022235-6ircruag 275 9 prevents prevent VBZ cord-022235-6ircruag 275 10 movement movement NN cord-022235-6ircruag 275 11 though though IN cord-022235-6ircruag 275 12 the the DT cord-022235-6ircruag 275 13 trans trans NNP cord-022235-6ircruag 275 14 cisternae cisternae NNP cord-022235-6ircruag 275 15 by by IN cord-022235-6ircruag 275 16 disrupting disrupt VBG cord-022235-6ircruag 275 17 a a DT cord-022235-6ircruag 275 18 proton proton NN cord-022235-6ircruag 275 19 gradient gradient NN cord-022235-6ircruag 275 20 maintained maintain VBN cord-022235-6ircruag 275 21 by by IN cord-022235-6ircruag 275 22 an an DT cord-022235-6ircruag 275 23 ATP ATP NNP cord-022235-6ircruag 275 24 - - HYPH cord-022235-6ircruag 275 25 dependent dependent JJ cord-022235-6ircruag 275 26 protein protein NN cord-022235-6ircruag 275 27 pump pump NN cord-022235-6ircruag 275 28 present present JJ cord-022235-6ircruag 275 29 in in IN cord-022235-6ircruag 275 30 Golgi Golgi NNP cord-022235-6ircruag 275 31 membranes membrane NNS cord-022235-6ircruag 275 32 ( ( -LRB- cord-022235-6ircruag 275 33 48 48 CD cord-022235-6ircruag 275 34 ) ) -RRB- cord-022235-6ircruag 275 35 , , , cord-022235-6ircruag 275 36 thus thus RB cord-022235-6ircruag 275 37 causing cause VBG cord-022235-6ircruag 275 38 the the DT cord-022235-6ircruag 275 39 pH ph NN cord-022235-6ircruag 275 40 of of IN cord-022235-6ircruag 275 41 the the DT cord-022235-6ircruag 275 42 normally normally RB cord-022235-6ircruag 275 43 acidic acidic JJ cord-022235-6ircruag 275 44 trans trans NNP cord-022235-6ircruag 275 45 Golgi Golgi NNP cord-022235-6ircruag 275 46 cisternae cisternae NNP cord-022235-6ircruag 275 47 to to TO cord-022235-6ircruag 275 48 rise rise VB cord-022235-6ircruag 275 49 . . . cord-022235-6ircruag 276 1 Saraste Saraste NNP cord-022235-6ircruag 276 2 and and CC cord-022235-6ircruag 276 3 Hedman Hedman NNP cord-022235-6ircruag 276 4 ( ( -LRB- cord-022235-6ircruag 276 5 994 994 CD cord-022235-6ircruag 276 6 ) ) -RRB- cord-022235-6ircruag 276 7 demonstrated demonstrate VBD cord-022235-6ircruag 276 8 that that IN cord-022235-6ircruag 276 9 mi mi CD cord-022235-6ircruag 276 10 gration gration NN cord-022235-6ircruag 276 11 between between IN cord-022235-6ircruag 276 12 different different JJ cord-022235-6ircruag 276 13 Golgi Golgi NNP cord-022235-6ircruag 276 14 cisternae cisternae NNP cord-022235-6ircruag 276 15 was be VBD cord-022235-6ircruag 276 16 blocked block VBN cord-022235-6ircruag 276 17 at at IN cord-022235-6ircruag 276 18 different different JJ cord-022235-6ircruag 276 19 critical critical JJ cord-022235-6ircruag 276 20 temperatures temperature NNS cord-022235-6ircruag 276 21 and and CC cord-022235-6ircruag 276 22 suggested suggest VBD cord-022235-6ircruag 276 23 that that IN cord-022235-6ircruag 276 24 this this DT cord-022235-6ircruag 276 25 might may MD cord-022235-6ircruag 276 26 be be VB cord-022235-6ircruag 276 27 caused cause VBN cord-022235-6ircruag 276 28 either either CC cord-022235-6ircruag 276 29 by by IN cord-022235-6ircruag 276 30 ATP ATP NNP cord-022235-6ircruag 276 31 depletion depletion NN cord-022235-6ircruag 276 32 or or CC cord-022235-6ircruag 276 33 by by IN cord-022235-6ircruag 276 34 changes change NNS cord-022235-6ircruag 276 35 in in IN cord-022235-6ircruag 276 36 membrane membrane NN cord-022235-6ircruag 276 37 fluidity fluidity NN cord-022235-6ircruag 276 38 . . . cord-022235-6ircruag 277 1 ATP ATP NNP cord-022235-6ircruag 277 2 may may MD cord-022235-6ircruag 277 3 be be VB cord-022235-6ircruag 277 4 required require VBN cord-022235-6ircruag 277 5 to to TO cord-022235-6ircruag 277 6 maintain maintain VB cord-022235-6ircruag 277 7 the the DT cord-022235-6ircruag 277 8 acidic acidic JJ cord-022235-6ircruag 277 9 pH ph NN cord-022235-6ircruag 277 10 of of IN cord-022235-6ircruag 277 11 trans trans NNP cord-022235-6ircruag 277 12 cisternae cisternae NNP cord-022235-6ircruag 277 13 , , , cord-022235-6ircruag 277 14 and and CC cord-022235-6ircruag 277 15 vesicle vesicle NN cord-022235-6ircruag 277 16 fission fission NN cord-022235-6ircruag 277 17 and and CC cord-022235-6ircruag 277 18 fusion fusion NN cord-022235-6ircruag 277 19 , , , cord-022235-6ircruag 277 20 which which WDT cord-022235-6ircruag 277 21 must must MD cord-022235-6ircruag 277 22 occur occur VB cord-022235-6ircruag 277 23 at at IN cord-022235-6ircruag 277 24 the the DT cord-022235-6ircruag 277 25 cisternal cisternal JJ cord-022235-6ircruag 277 26 membranes membrane NNS cord-022235-6ircruag 277 27 , , , cord-022235-6ircruag 277 28 can can MD cord-022235-6ircruag 277 29 probably probably RB cord-022235-6ircruag 277 30 only only RB cord-022235-6ircruag 277 31 occur occur VB cord-022235-6ircruag 277 32 when when WRB cord-022235-6ircruag 277 33 membrane membrane NN cord-022235-6ircruag 277 34 lipids lipid NNS cord-022235-6ircruag 277 35 are be VBP cord-022235-6ircruag 277 36 in in IN cord-022235-6ircruag 277 37 a a DT cord-022235-6ircruag 277 38 " " `` cord-022235-6ircruag 277 39 fluid fluid JJ cord-022235-6ircruag 277 40 " " '' cord-022235-6ircruag 277 41 state state NN cord-022235-6ircruag 277 42 above above IN cord-022235-6ircruag 277 43 the the DT cord-022235-6ircruag 277 44 phase phase NN cord-022235-6ircruag 277 45 transition transition NN cord-022235-6ircruag 277 46 temperature temperature NN cord-022235-6ircruag 277 47 . . . cord-022235-6ircruag 278 1 ATP ATP NNP cord-022235-6ircruag 278 2 and and CC cord-022235-6ircruag 278 3 soluble soluble JJ cord-022235-6ircruag 278 4 cytosolic cytosolic JJ cord-022235-6ircruag 278 5 factors factor NNS cord-022235-6ircruag 278 6 including include VBG cord-022235-6ircruag 278 7 a a DT cord-022235-6ircruag 278 8 74-kDa 74-kda CD cord-022235-6ircruag 278 9 , , , cord-022235-6ircruag 278 10 N n CD cord-022235-6ircruag 278 11 - - HYPH cord-022235-6ircruag 278 12 ethylmaleimide ethylmaleimide NN cord-022235-6ircruag 278 13 - - HYPH cord-022235-6ircruag 278 14 sensitive sensitive JJ cord-022235-6ircruag 278 15 protein protein NN cord-022235-6ircruag 278 16 ( ( -LRB- cord-022235-6ircruag 278 17 93a 93a NNS cord-022235-6ircruag 278 18 ) ) -RRB- cord-022235-6ircruag 278 19 are be VBP cord-022235-6ircruag 278 20 required require VBN cord-022235-6ircruag 278 21 in in IN cord-022235-6ircruag 278 22 both both DT cord-022235-6ircruag 278 23 early early JJ cord-022235-6ircruag 278 24 and and CC cord-022235-6ircruag 278 25 late late JJ cord-022235-6ircruag 278 26 stages stage NNS cord-022235-6ircruag 278 27 of of IN cord-022235-6ircruag 278 28 intra intra JJ cord-022235-6ircruag 278 29 - - JJ cord-022235-6ircruag 278 30 Golgi golgi JJ cord-022235-6ircruag 278 31 movement movement NN cord-022235-6ircruag 278 32 in in IN cord-022235-6ircruag 278 33 vitro vitro FW cord-022235-6ircruag 278 34 ( ( -LRB- cord-022235-6ircruag 278 35 39,40,685a,966 39,40,685a,966 CD cord-022235-6ircruag 278 36 ) ) -RRB- cord-022235-6ircruag 278 37 and and CC cord-022235-6ircruag 278 38 in in IN cord-022235-6ircruag 278 39 permeabilized permeabilized JJ cord-022235-6ircruag 278 40 cells cell NNS cord-022235-6ircruag 278 41 ( ( -LRB- cord-022235-6ircruag 278 42 62 62 CD cord-022235-6ircruag 278 43 ) ) -RRB- cord-022235-6ircruag 278 44 . . . cord-022235-6ircruag 279 1 Intriguingly intriguingly RB cord-022235-6ircruag 279 2 , , , cord-022235-6ircruag 279 3 soluble soluble JJ cord-022235-6ircruag 279 4 yeast yeast NN cord-022235-6ircruag 279 5 cell cell NN cord-022235-6ircruag 279 6 extracts extract NNS cord-022235-6ircruag 279 7 can can MD cord-022235-6ircruag 279 8 replace replace VB cord-022235-6ircruag 279 9 endogenous endogenous JJ cord-022235-6ircruag 279 10 cy cy NNP cord-022235-6ircruag 279 11 toplasmic toplasmic JJ cord-022235-6ircruag 279 12 components component NNS cord-022235-6ircruag 279 13 in in IN cord-022235-6ircruag 279 14 a a DT cord-022235-6ircruag 279 15 mammalian mammalian JJ cord-022235-6ircruag 279 16 cell cell NN cord-022235-6ircruag 279 17 - - HYPH cord-022235-6ircruag 279 18 derived derive VBN cord-022235-6ircruag 279 19 assay assay NN cord-022235-6ircruag 279 20 system system NN cord-022235-6ircruag 279 21 for for IN cord-022235-6ircruag 279 22 in in IN cord-022235-6ircruag 279 23 tra tra NNP cord-022235-6ircruag 279 24 - - HYPH cord-022235-6ircruag 279 25 Golgi Golgi NNP cord-022235-6ircruag 279 26 movement movement NN cord-022235-6ircruag 279 27 of of IN cord-022235-6ircruag 279 28 proteins protein NNS cord-022235-6ircruag 279 29 ( ( -LRB- cord-022235-6ircruag 279 30 268 268 CD cord-022235-6ircruag 279 31 ) ) -RRB- cord-022235-6ircruag 279 32 , , , cord-022235-6ircruag 279 33 raising raise VBG cord-022235-6ircruag 279 34 the the DT cord-022235-6ircruag 279 35 possibility possibility NN cord-022235-6ircruag 279 36 of of IN cord-022235-6ircruag 279 37 using use VBG cord-022235-6ircruag 279 38 ex ex NN cord-022235-6ircruag 279 39 tracts tract NNS cord-022235-6ircruag 279 40 from from IN cord-022235-6ircruag 279 41 yeast yeast NN cord-022235-6ircruag 279 42 sec sec NNP cord-022235-6ircruag 279 43 mutants mutant NNS cord-022235-6ircruag 279 44 blocked block VBN cord-022235-6ircruag 279 45 at at IN cord-022235-6ircruag 279 46 different different JJ cord-022235-6ircruag 279 47 stages stage NNS cord-022235-6ircruag 279 48 in in IN cord-022235-6ircruag 279 49 the the DT cord-022235-6ircruag 279 50 secretory secretory JJ cord-022235-6ircruag 279 51 pathway pathway NN cord-022235-6ircruag 279 52 to to TO cord-022235-6ircruag 279 53 define define VB cord-022235-6ircruag 279 54 the the DT cord-022235-6ircruag 279 55 role role NN cord-022235-6ircruag 279 56 of of IN cord-022235-6ircruag 279 57 the the DT cord-022235-6ircruag 279 58 corresponding corresponding JJ cord-022235-6ircruag 279 59 wild wild JJ cord-022235-6ircruag 279 60 - - HYPH cord-022235-6ircruag 279 61 type type NN cord-022235-6ircruag 279 62 gene gene NN cord-022235-6ircruag 279 63 products product NNS cord-022235-6ircruag 279 64 in in IN cord-022235-6ircruag 279 65 intra intra JJ cord-022235-6ircruag 279 66 - - JJ cord-022235-6ircruag 279 67 Golgi golgi JJ cord-022235-6ircruag 279 68 transport transport NN cord-022235-6ircruag 279 69 . . . cord-022235-6ircruag 280 1 Indirect indirect JJ cord-022235-6ircruag 280 2 evidence evidence NN cord-022235-6ircruag 280 3 based base VBN cord-022235-6ircruag 280 4 on on IN cord-022235-6ircruag 280 5 the the DT cord-022235-6ircruag 280 6 effects effect NNS cord-022235-6ircruag 280 7 of of IN cord-022235-6ircruag 280 8 GTP GTP NNP cord-022235-6ircruag 280 9 analogs analog NNS cord-022235-6ircruag 280 10 suggests suggest VBZ cord-022235-6ircruag 280 11 that that IN cord-022235-6ircruag 280 12 GTP GTP NNP cord-022235-6ircruag 280 13 is be VBZ cord-022235-6ircruag 280 14 also also RB cord-022235-6ircruag 280 15 needed need VBN cord-022235-6ircruag 280 16 for for IN cord-022235-6ircruag 280 17 intra intra JJ cord-022235-6ircruag 280 18 - - JJ cord-022235-6ircruag 280 19 Golgi golgi JJ cord-022235-6ircruag 280 20 movement movement NN cord-022235-6ircruag 280 21 of of IN cord-022235-6ircruag 280 22 secretory secretory JJ cord-022235-6ircruag 280 23 proteins protein NNS cord-022235-6ircruag 280 24 ( ( -LRB- cord-022235-6ircruag 280 25 710 710 CD cord-022235-6ircruag 280 26 ) ) -RRB- cord-022235-6ircruag 280 27 , , , cord-022235-6ircruag 280 28 and and CC cord-022235-6ircruag 280 29 studies study NNS cord-022235-6ircruag 280 30 with with IN cord-022235-6ircruag 280 31 antibodies antibody NNS cord-022235-6ircruag 280 32 against against IN cord-022235-6ircruag 280 33 a a DT cord-022235-6ircruag 280 34 yeast yeast NN cord-022235-6ircruag 280 35 GTP gtp NN cord-022235-6ircruag 280 36 binding bind VBG cord-022235-6ircruag 280 37 protein protein NN cord-022235-6ircruag 280 38 indicate indicate VBP cord-022235-6ircruag 280 39 that that IN cord-022235-6ircruag 280 40 a a DT cord-022235-6ircruag 280 41 similar similar JJ cord-022235-6ircruag 280 42 protein protein NN cord-022235-6ircruag 280 43 is be VBZ cord-022235-6ircruag 280 44 apparently apparently RB cord-022235-6ircruag 280 45 located locate VBN cord-022235-6ircruag 280 46 in in IN cord-022235-6ircruag 280 47 the the DT cord-022235-6ircruag 280 48 Golgi Golgi NNP cord-022235-6ircruag 280 49 apparatus apparatus NN cord-022235-6ircruag 280 50 in in IN cord-022235-6ircruag 280 51 multicellular multicellular JJ cord-022235-6ircruag 280 52 eukaryotes eukaryote NNS cord-022235-6ircruag 280 53 ( ( -LRB- cord-022235-6ircruag 280 54 1030 1030 CD cord-022235-6ircruag 280 55 ) ) -RRB- cord-022235-6ircruag 280 56 . . . cord-022235-6ircruag 281 1 The the DT cord-022235-6ircruag 281 2 significance significance NN cord-022235-6ircruag 281 3 of of IN cord-022235-6ircruag 281 4 this this DT cord-022235-6ircruag 281 5 observation observation NN cord-022235-6ircruag 281 6 is be VBZ cord-022235-6ircruag 281 7 not not RB cord-022235-6ircruag 281 8 understood understand VBN cord-022235-6ircruag 281 9 , , , cord-022235-6ircruag 281 10 but but CC cord-022235-6ircruag 281 11 GTP GTP NNP cord-022235-6ircruag 281 12 and and CC cord-022235-6ircruag 281 13 GTP GTP NNP cord-022235-6ircruag 281 14 binding bind VBG cord-022235-6ircruag 281 15 proteins protein NNS cord-022235-6ircruag 281 16 ( ( -LRB- cord-022235-6ircruag 281 17 G g NN cord-022235-6ircruag 281 18 proteins protein NNS cord-022235-6ircruag 281 19 ) ) -RRB- cord-022235-6ircruag 281 20 may may MD cord-022235-6ircruag 281 21 be be VB cord-022235-6ircruag 281 22 involved involve VBN cord-022235-6ircruag 281 23 in in IN cord-022235-6ircruag 281 24 maintaining maintain VBG cord-022235-6ircruag 281 25 vectorial vectorial JJ cord-022235-6ircruag 281 26 movement movement NN cord-022235-6ircruag 281 27 of of IN cord-022235-6ircruag 281 28 shuttle shuttle NN cord-022235-6ircruag 281 29 vesicles vesicle NNS cord-022235-6ircruag 281 30 or or CC cord-022235-6ircruag 281 31 in in IN cord-022235-6ircruag 281 32 vesicle vesicle NN cord-022235-6ircruag 281 33 recycling recycling NN cord-022235-6ircruag 281 34 ( ( -LRB- cord-022235-6ircruag 281 35 see see VB cord-022235-6ircruag 281 36 below below RB cord-022235-6ircruag 281 37 ) ) -RRB- cord-022235-6ircruag 281 38 . . . cord-022235-6ircruag 282 1 Surface surface NN cord-022235-6ircruag 282 2 components component NNS cord-022235-6ircruag 282 3 on on IN cord-022235-6ircruag 282 4 Golgi Golgi NNP cord-022235-6ircruag 282 5 membranes membrane NNS cord-022235-6ircruag 282 6 are be VBP cord-022235-6ircruag 282 7 also also RB cord-022235-6ircruag 282 8 required require VBN cord-022235-6ircruag 282 9 for for IN cord-022235-6ircruag 282 10 intra intra JJ cord-022235-6ircruag 282 11 - - JJ cord-022235-6ircruag 282 12 Golgi golgi JJ cord-022235-6ircruag 282 13 transport transport NN cord-022235-6ircruag 282 14 ( ( -LRB- cord-022235-6ircruag 282 15 3,39,40 3,39,40 CD cord-022235-6ircruag 282 16 ) ) -RRB- cord-022235-6ircruag 282 17 . . . cord-022235-6ircruag 283 1 A a DT cord-022235-6ircruag 283 2 recep recep NN cord-022235-6ircruag 283 3 tor tor NN cord-022235-6ircruag 283 4 may may MD cord-022235-6ircruag 283 5 recognize recognize VB cord-022235-6ircruag 283 6 the the DT cord-022235-6ircruag 283 7 protein protein NN cord-022235-6ircruag 283 8 coat coat NN cord-022235-6ircruag 283 9 on on IN cord-022235-6ircruag 283 10 the the DT cord-022235-6ircruag 283 11 Golgi Golgi NNP cord-022235-6ircruag 283 12 transport transport NN cord-022235-6ircruag 283 13 vesicles vesicle NNS cord-022235-6ircruag 283 14 . . . cord-022235-6ircruag 284 1 Balch Balch NNP cord-022235-6ircruag 284 2 et et NNP cord-022235-6ircruag 284 3 al al NNP cord-022235-6ircruag 284 4 . . . cord-022235-6ircruag 285 1 ( ( -LRB- cord-022235-6ircruag 285 2 40 40 CD cord-022235-6ircruag 285 3 ) ) -RRB- cord-022235-6ircruag 285 4 considered consider VBD cord-022235-6ircruag 285 5 that that IN cord-022235-6ircruag 285 6 the the DT cord-022235-6ircruag 285 7 migration migration NN cord-022235-6ircruag 285 8 of of IN cord-022235-6ircruag 285 9 secretory secretory JJ cord-022235-6ircruag 285 10 proteins protein NNS cord-022235-6ircruag 285 11 from from IN cord-022235-6ircruag 285 12 one one CD cord-022235-6ircruag 285 13 cisterna cisterna NN cord-022235-6ircruag 285 14 to to IN cord-022235-6ircruag 285 15 another another DT cord-022235-6ircruag 285 16 depends depend VBZ cord-022235-6ircruag 285 17 on on IN cord-022235-6ircruag 285 18 three three CD cord-022235-6ircruag 285 19 separate separate JJ cord-022235-6ircruag 285 20 events event NNS cord-022235-6ircruag 285 21 : : : cord-022235-6ircruag 285 22 ( ( -LRB- cord-022235-6ircruag 285 23 i i NN cord-022235-6ircruag 285 24 ) ) -RRB- cord-022235-6ircruag 285 25 Secretory secretory NN cord-022235-6ircruag 285 26 proteins protein NNS cord-022235-6ircruag 285 27 are be VBP cord-022235-6ircruag 285 28 primed prime VBN cord-022235-6ircruag 285 29 to to TO cord-022235-6ircruag 285 30 make make VB cord-022235-6ircruag 285 31 them -PRON- PRP cord-022235-6ircruag 285 32 competent competent JJ cord-022235-6ircruag 285 33 or or CC cord-022235-6ircruag 285 34 available available JJ cord-022235-6ircruag 285 35 for for IN cord-022235-6ircruag 285 36 transport transport NN cord-022235-6ircruag 285 37 . . . cord-022235-6ircruag 286 1 Priming prime VBG cord-022235-6ircruag 286 2 probably probably RB cord-022235-6ircruag 286 3 involves involve VBZ cord-022235-6ircruag 286 4 the the DT cord-022235-6ircruag 286 5 migration migration NN cord-022235-6ircruag 286 6 of of IN cord-022235-6ircruag 286 7 secretory secretory JJ cord-022235-6ircruag 286 8 proteins protein NNS cord-022235-6ircruag 286 9 to to IN cord-022235-6ircruag 286 10 regions region NNS cord-022235-6ircruag 286 11 of of IN cord-022235-6ircruag 286 12 the the DT cord-022235-6ircruag 286 13 cisternae cisternae NNP cord-022235-6ircruag 286 14 where where WRB cord-022235-6ircruag 286 15 budding budding NN cord-022235-6ircruag 286 16 occurs occur VBZ cord-022235-6ircruag 286 17 , , , cord-022235-6ircruag 286 18 princi princi NNP cord-022235-6ircruag 286 19 - - HYPH cord-022235-6ircruag 286 20 pally pally NNP cord-022235-6ircruag 286 21 at at IN cord-022235-6ircruag 286 22 the the DT cord-022235-6ircruag 286 23 outer outer JJ cord-022235-6ircruag 286 24 rims rim NNS cord-022235-6ircruag 286 25 . . . cord-022235-6ircruag 287 1 This this DT cord-022235-6ircruag 287 2 step step NN cord-022235-6ircruag 287 3 might may MD cord-022235-6ircruag 287 4 depend depend VB cord-022235-6ircruag 287 5 on on IN cord-022235-6ircruag 287 6 interactions interaction NNS cord-022235-6ircruag 287 7 be be VB cord-022235-6ircruag 287 8 tween tween VBN cord-022235-6ircruag 287 9 the the DT cord-022235-6ircruag 287 10 secretory secretory JJ cord-022235-6ircruag 287 11 protein protein NN cord-022235-6ircruag 287 12 and and CC cord-022235-6ircruag 287 13 receptors receptor NNS cord-022235-6ircruag 287 14 migrating migrate VBG cord-022235-6ircruag 287 15 to to IN cord-022235-6ircruag 287 16 the the DT cord-022235-6ircruag 287 17 budding bud VBG cord-022235-6ircruag 287 18 areas area NNS cord-022235-6ircruag 287 19 or or CC cord-022235-6ircruag 287 20 may may MD cord-022235-6ircruag 287 21 rely rely VB cord-022235-6ircruag 287 22 on on IN cord-022235-6ircruag 287 23 free free JJ cord-022235-6ircruag 287 24 diffusion diffusion NN cord-022235-6ircruag 287 25 within within IN cord-022235-6ircruag 287 26 the the DT cord-022235-6ircruag 287 27 cisternal cisternal JJ cord-022235-6ircruag 287 28 membrane membrane NN cord-022235-6ircruag 287 29 or or CC cord-022235-6ircruag 287 30 lumen lumen NN cord-022235-6ircruag 287 31 . . . cord-022235-6ircruag 288 1 Segregation segregation NN cord-022235-6ircruag 288 2 may may MD cord-022235-6ircruag 288 3 depend depend VB cord-022235-6ircruag 288 4 on on IN cord-022235-6ircruag 288 5 signals signal NNS cord-022235-6ircruag 288 6 generated generate VBN cord-022235-6ircruag 288 7 by by IN cord-022235-6ircruag 288 8 processing process VBG cord-022235-6ircruag 288 9 enzymes enzyme NNS cord-022235-6ircruag 288 10 in in IN cord-022235-6ircruag 288 11 individual individual JJ cord-022235-6ircruag 288 12 Golgi Golgi NNP cord-022235-6ircruag 288 13 cisternae cisternae NNP cord-022235-6ircruag 288 14 , , , cord-022235-6ircruag 288 15 but but CC cord-022235-6ircruag 288 16 most most JJS cord-022235-6ircruag 288 17 experiments experiment NNS cord-022235-6ircruag 288 18 in in IN cord-022235-6ircruag 288 19 which which WDT cord-022235-6ircruag 288 20 processing processing NN cord-022235-6ircruag 288 21 inhibitors inhibitor NNS cord-022235-6ircruag 288 22 have have VBP cord-022235-6ircruag 288 23 been be VBN cord-022235-6ircruag 288 24 used use VBN cord-022235-6ircruag 288 25 do do VBP cord-022235-6ircruag 288 26 not not RB cord-022235-6ircruag 288 27 support support VB cord-022235-6ircruag 288 28 this this DT cord-022235-6ircruag 288 29 idea idea NN cord-022235-6ircruag 288 30 and and CC cord-022235-6ircruag 288 31 show show VBP cord-022235-6ircruag 288 32 instead instead RB cord-022235-6ircruag 288 33 that that DT cord-022235-6ircruag 288 34 processing processing NN cord-022235-6ircruag 288 35 is be VBZ cord-022235-6ircruag 288 36 nonessential nonessential JJ cord-022235-6ircruag 288 37 for for IN cord-022235-6ircruag 288 38 secretory secretory JJ cord-022235-6ircruag 288 39 pro pro FW cord-022235-6ircruag 288 40 tein tein NNP cord-022235-6ircruag 288 41 targeting target VBG cord-022235-6ircruag 288 42 . . . cord-022235-6ircruag 289 1 Some some DT cord-022235-6ircruag 289 2 mutationally mutationally RB cord-022235-6ircruag 289 3 altered alter VBN cord-022235-6ircruag 289 4 secretory secretory JJ cord-022235-6ircruag 289 5 proteins protein NNS cord-022235-6ircruag 289 6 , , , cord-022235-6ircruag 289 7 how how WRB cord-022235-6ircruag 289 8 ever ever RB cord-022235-6ircruag 289 9 , , , cord-022235-6ircruag 289 10 transit transit NN cord-022235-6ircruag 289 11 normally normally RB cord-022235-6ircruag 289 12 through through IN cord-022235-6ircruag 289 13 the the DT cord-022235-6ircruag 289 14 early early JJ cord-022235-6ircruag 289 15 stages stage NNS cord-022235-6ircruag 289 16 of of IN cord-022235-6ircruag 289 17 the the DT cord-022235-6ircruag 289 18 secretory secretory JJ cord-022235-6ircruag 289 19 path path NN cord-022235-6ircruag 289 20 way way NN cord-022235-6ircruag 289 21 and and CC cord-022235-6ircruag 289 22 yet yet RB cord-022235-6ircruag 289 23 are be VBP cord-022235-6ircruag 289 24 not not RB cord-022235-6ircruag 289 25 transported transport VBN cord-022235-6ircruag 289 26 through through IN cord-022235-6ircruag 289 27 the the DT cord-022235-6ircruag 289 28 Golgi Golgi NNP cord-022235-6ircruag 289 29 ( ( -LRB- cord-022235-6ircruag 289 30 344,1040 344,1040 NNP cord-022235-6ircruag 289 31 ) ) -RRB- cord-022235-6ircruag 289 32 . . . cord-022235-6ircruag 290 1 Perhaps perhaps RB cord-022235-6ircruag 290 2 secretory secretory JJ cord-022235-6ircruag 290 3 proteins protein NNS cord-022235-6ircruag 290 4 must must MD cord-022235-6ircruag 290 5 fold fold VB cord-022235-6ircruag 290 6 into into IN cord-022235-6ircruag 290 7 a a DT cord-022235-6ircruag 290 8 particular particular JJ cord-022235-6ircruag 290 9 conformation conformation NN cord-022235-6ircruag 290 10 to to TO cord-022235-6ircruag 290 11 be be VB cord-022235-6ircruag 290 12 competent competent JJ cord-022235-6ircruag 290 13 for for IN cord-022235-6ircruag 290 14 movement movement NN cord-022235-6ircruag 290 15 between between IN cord-022235-6ircruag 290 16 Golgi Golgi NNP cord-022235-6ircruag 290 17 cisternae cisternae NNP cord-022235-6ircruag 290 18 . . . cord-022235-6ircruag 291 1 The the DT cord-022235-6ircruag 291 2 accu accu JJ cord-022235-6ircruag 291 3 mulation mulation NN cord-022235-6ircruag 291 4 of of IN cord-022235-6ircruag 291 5 such such JJ cord-022235-6ircruag 291 6 abnormal abnormal JJ cord-022235-6ircruag 291 7 proteins protein NNS cord-022235-6ircruag 291 8 in in IN cord-022235-6ircruag 291 9 the the DT cord-022235-6ircruag 291 10 Golgi Golgi NNP cord-022235-6ircruag 291 11 may may MD cord-022235-6ircruag 291 12 cause cause VB cord-022235-6ircruag 291 13 it -PRON- PRP cord-022235-6ircruag 291 14 to to TO cord-022235-6ircruag 291 15 become become VB cord-022235-6ircruag 291 16 distended distended JJ cord-022235-6ircruag 291 17 , , , cord-022235-6ircruag 291 18 but but CC cord-022235-6ircruag 291 19 this this DT cord-022235-6ircruag 291 20 does do VBZ cord-022235-6ircruag 291 21 not not RB cord-022235-6ircruag 291 22 drastically drastically RB cord-022235-6ircruag 291 23 affect affect VB cord-022235-6ircruag 291 24 intra intra JJ cord-022235-6ircruag 291 25 - - JJ cord-022235-6ircruag 291 26 Golgi golgi JJ cord-022235-6ircruag 291 27 movement movement NN cord-022235-6ircruag 291 28 and and CC cord-022235-6ircruag 291 29 secretion secretion NN cord-022235-6ircruag 291 30 of of IN cord-022235-6ircruag 291 31 other other JJ cord-022235-6ircruag 291 32 secretory secretory JJ cord-022235-6ircruag 291 33 proteins protein NNS cord-022235-6ircruag 291 34 ( ( -LRB- cord-022235-6ircruag 291 35 344 344 CD cord-022235-6ircruag 291 36 ) ) -RRB- cord-022235-6ircruag 291 37 . . . cord-022235-6ircruag 292 1 ( ( -LRB- cord-022235-6ircruag 292 2 ii ii NN cord-022235-6ircruag 292 3 ) ) -RRB- cord-022235-6ircruag 293 1 Vesicles vesicle NNS cord-022235-6ircruag 293 2 move move VBP cord-022235-6ircruag 293 3 from from IN cord-022235-6ircruag 293 4 one one CD cord-022235-6ircruag 293 5 cisterna cisterna NN cord-022235-6ircruag 293 6 to to IN cord-022235-6ircruag 293 7 another another DT cord-022235-6ircruag 293 8 . . . cord-022235-6ircruag 294 1 Vesicle Vesicle NNP cord-022235-6ircruag 294 2 migration migration NN cord-022235-6ircruag 294 3 be be VB cord-022235-6ircruag 294 4 tween tween NN cord-022235-6ircruag 294 5 Golgi Golgi NNP cord-022235-6ircruag 294 6 cisternae cisternae NNP cord-022235-6ircruag 294 7 may may MD cord-022235-6ircruag 294 8 be be VB cord-022235-6ircruag 294 9 either either CC cord-022235-6ircruag 294 10 vectorial vectorial JJ cord-022235-6ircruag 294 11 or or CC cord-022235-6ircruag 294 12 random random JJ cord-022235-6ircruag 294 13 . . . cord-022235-6ircruag 295 1 Vectorial Vectorial NNP cord-022235-6ircruag 295 2 movement movement NN cord-022235-6ircruag 295 3 is be VBZ cord-022235-6ircruag 295 4 more more RBR cord-022235-6ircruag 295 5 compatible compatible JJ cord-022235-6ircruag 295 6 with with IN cord-022235-6ircruag 295 7 traditional traditional JJ cord-022235-6ircruag 295 8 views view NNS cord-022235-6ircruag 295 9 on on IN cord-022235-6ircruag 295 10 the the DT cord-022235-6ircruag 295 11 strict strict JJ cord-022235-6ircruag 295 12 sequence sequence NN cord-022235-6ircruag 295 13 of of IN cord-022235-6ircruag 295 14 events event NNS cord-022235-6ircruag 295 15 in in IN cord-022235-6ircruag 295 16 secretory secretory JJ cord-022235-6ircruag 295 17 protein protein NN cord-022235-6ircruag 295 18 processing processing NN cord-022235-6ircruag 295 19 and and CC cord-022235-6ircruag 295 20 is be VBZ cord-022235-6ircruag 295 21 supported support VBN cord-022235-6ircruag 295 22 by by IN cord-022235-6ircruag 295 23 the the DT cord-022235-6ircruag 295 24 observation observation NN cord-022235-6ircruag 295 25 that that IN cord-022235-6ircruag 295 26 the the DT cord-022235-6ircruag 295 27 likelihood likelihood NN cord-022235-6ircruag 295 28 of of IN cord-022235-6ircruag 295 29 forward forward JJ cord-022235-6ircruag 295 30 transfer transfer NN cord-022235-6ircruag 295 31 is be VBZ cord-022235-6ircruag 295 32 at at RB cord-022235-6ircruag 295 33 least least RBS cord-022235-6ircruag 295 34 five five CD cord-022235-6ircruag 295 35 times time NNS cord-022235-6ircruag 295 36 greater great JJR cord-022235-6ircruag 295 37 than than IN cord-022235-6ircruag 295 38 that that DT cord-022235-6ircruag 295 39 of of IN cord-022235-6ircruag 295 40 lateral lateral JJ cord-022235-6ircruag 295 41 movement movement NN cord-022235-6ircruag 295 42 in in IN cord-022235-6ircruag 295 43 Golgi Golgi NNP cord-022235-6ircruag 295 44 of of IN cord-022235-6ircruag 295 45 fused fuse VBN cord-022235-6ircruag 295 46 cells cell NNS cord-022235-6ircruag 295 47 ( ( -LRB- cord-022235-6ircruag 295 48 969 969 CD cord-022235-6ircruag 295 49 , , , cord-022235-6ircruag 295 50 970 970 CD cord-022235-6ircruag 295 51 ) ) -RRB- cord-022235-6ircruag 295 52 . . . cord-022235-6ircruag 296 1 This this DT cord-022235-6ircruag 296 2 implies imply VBZ cord-022235-6ircruag 296 3 that that IN cord-022235-6ircruag 296 4 there there EX cord-022235-6ircruag 296 5 are be VBP cord-022235-6ircruag 296 6 receptors receptor NNS cord-022235-6ircruag 296 7 on on IN cord-022235-6ircruag 296 8 the the DT cord-022235-6ircruag 296 9 surfaces surface NNS cord-022235-6ircruag 296 10 of of IN cord-022235-6ircruag 296 11 Golgi Golgi NNP cord-022235-6ircruag 296 12 cisternae cisternae NNP cord-022235-6ircruag 296 13 which which WDT cord-022235-6ircruag 296 14 recognize recognize VBP cord-022235-6ircruag 296 15 vesicles vesicle NNS cord-022235-6ircruag 296 16 budding bud VBG cord-022235-6ircruag 296 17 from from IN cord-022235-6ircruag 296 18 the the DT cord-022235-6ircruag 296 19 pre pre JJ cord-022235-6ircruag 296 20 ceding cede VBG cord-022235-6ircruag 296 21 cisterna cisterna NNP cord-022235-6ircruag 296 22 in in IN cord-022235-6ircruag 296 23 the the DT cord-022235-6ircruag 296 24 chain chain NN cord-022235-6ircruag 296 25 . . . cord-022235-6ircruag 297 1 Nonetheless nonetheless RB cord-022235-6ircruag 297 2 , , , cord-022235-6ircruag 297 3 the the DT cord-022235-6ircruag 297 4 topology topology NN cord-022235-6ircruag 297 5 in in IN cord-022235-6ircruag 297 6 the the DT cord-022235-6ircruag 297 7 Golgi Golgi NNP cord-022235-6ircruag 297 8 complex complex NN cord-022235-6ircruag 297 9 is be VBZ cord-022235-6ircruag 297 10 well well RB cord-022235-6ircruag 297 11 maintained maintain VBN cord-022235-6ircruag 297 12 , , , cord-022235-6ircruag 297 13 possibly possibly RB cord-022235-6ircruag 297 14 because because IN cord-022235-6ircruag 297 15 the the DT cord-022235-6ircruag 297 16 cytoskeleton cytoskeleton NN cord-022235-6ircruag 297 17 pre pre VBD cord-022235-6ircruag 297 18 vents vent NNS cord-022235-6ircruag 297 19 cisternae cisternae JJ cord-022235-6ircruag 297 20 from from IN cord-022235-6ircruag 297 21 coming come VBG cord-022235-6ircruag 297 22 into into IN cord-022235-6ircruag 297 23 direct direct JJ cord-022235-6ircruag 297 24 contact contact NN cord-022235-6ircruag 297 25 . . . cord-022235-6ircruag 298 1 Another another DT cord-022235-6ircruag 298 2 puzzling puzzling JJ cord-022235-6ircruag 298 3 feature feature NN cord-022235-6ircruag 298 4 of of IN cord-022235-6ircruag 298 5 vesicular vesicular JJ cord-022235-6ircruag 298 6 intra intra JJ cord-022235-6ircruag 298 7 - - JJ cord-022235-6ircruag 298 8 Golgi golgi JJ cord-022235-6ircruag 298 9 transport transport NN cord-022235-6ircruag 298 10 is be VBZ cord-022235-6ircruag 298 11 that that DT cord-022235-6ircruag 298 12 small small JJ cord-022235-6ircruag 298 13 transport transport NN cord-022235-6ircruag 298 14 vesi vesi NNP cord-022235-6ircruag 298 15 cles cles NNP cord-022235-6ircruag 298 16 would would MD cord-022235-6ircruag 298 17 be be VB cord-022235-6ircruag 298 18 expected expect VBN cord-022235-6ircruag 298 19 to to TO cord-022235-6ircruag 298 20 diffuse diffuse VB cord-022235-6ircruag 298 21 away away RB cord-022235-6ircruag 298 22 from from IN cord-022235-6ircruag 298 23 the the DT cord-022235-6ircruag 298 24 Golgi Golgi NNP cord-022235-6ircruag 298 25 complex complex NN cord-022235-6ircruag 298 26 . . . cord-022235-6ircruag 299 1 The the DT cord-022235-6ircruag 299 2 cytoskeleton cytoskeleton NN cord-022235-6ircruag 299 3 may may MD cord-022235-6ircruag 299 4 restrict restrict VB cord-022235-6ircruag 299 5 the the DT cord-022235-6ircruag 299 6 movement movement NN cord-022235-6ircruag 299 7 of of IN cord-022235-6ircruag 299 8 Golgi Golgi NNP cord-022235-6ircruag 299 9 vesicles vesicle NNS cord-022235-6ircruag 299 10 and and CC cord-022235-6ircruag 299 11 may may MD cord-022235-6ircruag 299 12 even even RB cord-022235-6ircruag 299 13 play play VB cord-022235-6ircruag 299 14 a a DT cord-022235-6ircruag 299 15 more more RBR cord-022235-6ircruag 299 16 positive positive JJ cord-022235-6ircruag 299 17 role role NN cord-022235-6ircruag 299 18 in in IN cord-022235-6ircruag 299 19 directing direct VBG cord-022235-6ircruag 299 20 vesicles vesicle NNS cord-022235-6ircruag 299 21 between between IN cord-022235-6ircruag 299 22 cister cister NNP cord-022235-6ircruag 299 23 nae nae NNP cord-022235-6ircruag 299 24 . . . cord-022235-6ircruag 300 1 However however RB cord-022235-6ircruag 300 2 , , , cord-022235-6ircruag 300 3 although although IN cord-022235-6ircruag 300 4 movement movement NN cord-022235-6ircruag 300 5 along along IN cord-022235-6ircruag 300 6 microtubules microtubule NNS cord-022235-6ircruag 300 7 has have VBZ cord-022235-6ircruag 300 8 been be VBN cord-022235-6ircruag 300 9 well well RB cord-022235-6ircruag 300 10 documented document VBN cord-022235-6ircruag 300 11 for for IN cord-022235-6ircruag 300 12 large large JJ cord-022235-6ircruag 300 13 organelles organelle NNS cord-022235-6ircruag 300 14 ( ( -LRB- cord-022235-6ircruag 300 15 1136 1136 CD cord-022235-6ircruag 300 16 ) ) -RRB- cord-022235-6ircruag 300 17 and and CC cord-022235-6ircruag 300 18 may may MD cord-022235-6ircruag 300 19 be be VB cord-022235-6ircruag 300 20 important important JJ cord-022235-6ircruag 300 21 in in IN cord-022235-6ircruag 300 22 the the DT cord-022235-6ircruag 300 23 sorting sorting NN cord-022235-6ircruag 300 24 of of IN cord-022235-6ircruag 300 25 some some DT cord-022235-6ircruag 300 26 proteins protein NNS cord-022235-6ircruag 300 27 leaving leave VBG cord-022235-6ircruag 300 28 the the DT cord-022235-6ircruag 300 29 Golgi Golgi NNP cord-022235-6ircruag 300 30 complex complex NN cord-022235-6ircruag 300 31 ( ( -LRB- cord-022235-6ircruag 300 32 Section section NN cord-022235-6ircruag 300 33 V.G.3 v.g.3 NN cord-022235-6ircruag 300 34 ) ) -RRB- cord-022235-6ircruag 300 35 , , , cord-022235-6ircruag 300 36 there there EX cord-022235-6ircruag 300 37 is be VBZ cord-022235-6ircruag 300 38 no no DT cord-022235-6ircruag 300 39 evidence evidence NN cord-022235-6ircruag 300 40 that that IN cord-022235-6ircruag 300 41 it -PRON- PRP cord-022235-6ircruag 300 42 could could MD cord-022235-6ircruag 300 43 play play VB cord-022235-6ircruag 300 44 more more JJR cord-022235-6ircruag 300 45 than than IN cord-022235-6ircruag 300 46 a a DT cord-022235-6ircruag 300 47 minor minor JJ cord-022235-6ircruag 300 48 role role NN cord-022235-6ircruag 300 49 in in IN cord-022235-6ircruag 300 50 the the DT cord-022235-6ircruag 300 51 movement movement NN cord-022235-6ircruag 300 52 of of IN cord-022235-6ircruag 300 53 vesicles vesicle NNS cord-022235-6ircruag 300 54 over over IN cord-022235-6ircruag 300 55 the the DT cord-022235-6ircruag 300 56 very very RB cord-022235-6ircruag 300 57 short short JJ cord-022235-6ircruag 300 58 distances distance NNS cord-022235-6ircruag 300 59 which which WDT cord-022235-6ircruag 300 60 separate separate VBP cord-022235-6ircruag 300 61 Golgi Golgi NNP cord-022235-6ircruag 300 62 cisternae cisternae NNP cord-022235-6ircruag 300 63 ( ( -LRB- cord-022235-6ircruag 300 64 139 139 CD cord-022235-6ircruag 300 65 ) ) -RRB- cord-022235-6ircruag 300 66 . . . cord-022235-6ircruag 301 1 Furthermore furthermore RB cord-022235-6ircruag 301 2 , , , cord-022235-6ircruag 301 3 cell cell NN cord-022235-6ircruag 301 4 fusion fusion NN cord-022235-6ircruag 301 5 studies study NNS cord-022235-6ircruag 301 6 by by IN cord-022235-6ircruag 301 7 Rothman Rothman NNP cord-022235-6ircruag 301 8 et et NNP cord-022235-6ircruag 301 9 al al NNP cord-022235-6ircruag 301 10 . . . cord-022235-6ircruag 302 1 ( ( -LRB- cord-022235-6ircruag 302 2 969 969 CD cord-022235-6ircruag 302 3 ) ) -RRB- cord-022235-6ircruag 302 4 show show VBP cord-022235-6ircruag 302 5 that that IN cord-022235-6ircruag 302 6 inter inter JJ cord-022235-6ircruag 302 7 - - JJ cord-022235-6ircruag 302 8 Golgi golgi JJ cord-022235-6ircruag 302 9 movement movement NN cord-022235-6ircruag 302 10 of of IN cord-022235-6ircruag 302 11 VSV VSV NNP cord-022235-6ircruag 302 12 G G NNP cord-022235-6ircruag 302 13 protein protein NN cord-022235-6ircruag 302 14 can can MD cord-022235-6ircruag 302 15 occur occur VB cord-022235-6ircruag 302 16 , , , cord-022235-6ircruag 302 17 indicating indicate VBG cord-022235-6ircruag 302 18 either either CC cord-022235-6ircruag 302 19 fusion fusion NN cord-022235-6ircruag 302 20 of of IN cord-022235-6ircruag 302 21 the the DT cord-022235-6ircruag 302 22 two two CD cord-022235-6ircruag 302 23 Golgi Golgi NNP cord-022235-6ircruag 302 24 com com NN cord-022235-6ircruag 302 25 plexes plexe NNS cord-022235-6ircruag 302 26 or or CC cord-022235-6ircruag 302 27 , , , cord-022235-6ircruag 302 28 more more RBR cord-022235-6ircruag 302 29 likely likely JJ cord-022235-6ircruag 302 30 , , , cord-022235-6ircruag 303 1 that that DT cord-022235-6ircruag 303 2 movement movement NN cord-022235-6ircruag 303 3 between between IN cord-022235-6ircruag 303 4 Golgi Golgi NNP cord-022235-6ircruag 303 5 cisternae cisternae NNP cord-022235-6ircruag 303 6 is be VBZ cord-022235-6ircruag 303 7 dissociative dissociative JJ cord-022235-6ircruag 303 8 ; ; , cord-022235-6ircruag 303 9 i.e. i.e. FW cord-022235-6ircruag 303 10 , , , cord-022235-6ircruag 303 11 the the DT cord-022235-6ircruag 303 12 vesicles vesicle NNS cord-022235-6ircruag 303 13 do do VBP cord-022235-6ircruag 303 14 indeed indeed RB cord-022235-6ircruag 303 15 diffuse diffuse VB cord-022235-6ircruag 303 16 into into IN cord-022235-6ircruag 303 17 the the DT cord-022235-6ircruag 303 18 cytoplasm cytoplasm NN cord-022235-6ircruag 303 19 . . . cord-022235-6ircruag 304 1 ( ( -LRB- cord-022235-6ircruag 304 2 iii iii NN cord-022235-6ircruag 304 3 ) ) -RRB- cord-022235-6ircruag 305 1 Vesicle Vesicle NNP cord-022235-6ircruag 305 2 fusion fusion NN cord-022235-6ircruag 305 3 with with IN cord-022235-6ircruag 305 4 the the DT cord-022235-6ircruag 305 5 membrane membrane NN cord-022235-6ircruag 305 6 of of IN cord-022235-6ircruag 305 7 the the DT cord-022235-6ircruag 305 8 acceptor acceptor NN cord-022235-6ircruag 305 9 cisterna cisterna NNP cord-022235-6ircruag 305 10 and and CC cord-022235-6ircruag 306 1 re re IN cord-022235-6ircruag 306 2 lease lease NN cord-022235-6ircruag 306 3 of of IN cord-022235-6ircruag 306 4 vesicle vesicle NN cord-022235-6ircruag 306 5 contents content NNS cord-022235-6ircruag 306 6 into into IN cord-022235-6ircruag 306 7 the the DT cord-022235-6ircruag 306 8 lumen lumen NN cord-022235-6ircruag 306 9 of of IN cord-022235-6ircruag 306 10 the the DT cord-022235-6ircruag 306 11 cisterna cisterna NNP cord-022235-6ircruag 306 12 occurs occur VBZ cord-022235-6ircruag 306 13 . . . cord-022235-6ircruag 307 1 This this DT cord-022235-6ircruag 307 2 model model NN cord-022235-6ircruag 307 3 for for IN cord-022235-6ircruag 307 4 intra intra JJ cord-022235-6ircruag 307 5 - - JJ cord-022235-6ircruag 307 6 Golgi golgi JJ cord-022235-6ircruag 307 7 transport transport NN cord-022235-6ircruag 307 8 fits fit VBZ cord-022235-6ircruag 307 9 many many JJ cord-022235-6ircruag 307 10 experimental experimental JJ cord-022235-6ircruag 307 11 observa observa NNP cord-022235-6ircruag 307 12 tions tion NNS cord-022235-6ircruag 307 13 , , , cord-022235-6ircruag 307 14 but but CC cord-022235-6ircruag 307 15 further further JJ cord-022235-6ircruag 307 16 studies study NNS cord-022235-6ircruag 307 17 are be VBP cord-022235-6ircruag 307 18 required require VBN cord-022235-6ircruag 307 19 to to TO cord-022235-6ircruag 307 20 define define VB cord-022235-6ircruag 307 21 clearly clearly RB cord-022235-6ircruag 307 22 the the DT cord-022235-6ircruag 307 23 steps step NNS cord-022235-6ircruag 307 24 involved involve VBN cord-022235-6ircruag 307 25 . . . cord-022235-6ircruag 308 1 For for IN cord-022235-6ircruag 308 2 example example NN cord-022235-6ircruag 308 3 , , , cord-022235-6ircruag 308 4 ATP ATP NNP cord-022235-6ircruag 308 5 may may MD cord-022235-6ircruag 308 6 be be VB cord-022235-6ircruag 308 7 required require VBN cord-022235-6ircruag 308 8 for for IN cord-022235-6ircruag 308 9 vesicle vesicle NN cord-022235-6ircruag 308 10 fission fission NN cord-022235-6ircruag 308 11 and and CC cord-022235-6ircruag 308 12 fusion fusion NN cord-022235-6ircruag 308 13 , , , cord-022235-6ircruag 308 14 as as RB cord-022235-6ircruag 308 15 well well RB cord-022235-6ircruag 308 16 as as IN cord-022235-6ircruag 308 17 for for IN cord-022235-6ircruag 308 18 reducing reduce VBG cord-022235-6ircruag 308 19 the the DT cord-022235-6ircruag 308 20 pH pH NNP cord-022235-6ircruag 308 21 in in IN cord-022235-6ircruag 308 22 trans trans NNP cord-022235-6ircruag 308 23 Golgi Golgi NNP cord-022235-6ircruag 308 24 compartments compartment NNS cord-022235-6ircruag 308 25 , , , cord-022235-6ircruag 308 26 but but CC cord-022235-6ircruag 308 27 this this DT cord-022235-6ircruag 308 28 has have VBZ cord-022235-6ircruag 308 29 not not RB cord-022235-6ircruag 308 30 been be VBN cord-022235-6ircruag 308 31 proven prove VBN cord-022235-6ircruag 308 32 , , , cord-022235-6ircruag 308 33 and and CC cord-022235-6ircruag 308 34 the the DT cord-022235-6ircruag 308 35 nature nature NN cord-022235-6ircruag 308 36 and and CC cord-022235-6ircruag 308 37 role role NN cord-022235-6ircruag 308 38 of of IN cord-022235-6ircruag 308 39 some some DT cord-022235-6ircruag 308 40 of of IN cord-022235-6ircruag 308 41 the the DT cord-022235-6ircruag 308 42 cytosolic cytosolic JJ cord-022235-6ircruag 308 43 components component NNS cord-022235-6ircruag 308 44 required require VBN cord-022235-6ircruag 308 45 in in RP cord-022235-6ircruag 308 46 in in IN cord-022235-6ircruag 308 47 vitro vitro FW cord-022235-6ircruag 308 48 assays assay NNS cord-022235-6ircruag 308 49 have have VBP cord-022235-6ircruag 308 50 yet yet RB cord-022235-6ircruag 308 51 to to TO cord-022235-6ircruag 308 52 be be VB cord-022235-6ircruag 308 53 determined determine VBN cord-022235-6ircruag 308 54 . . . cord-022235-6ircruag 309 1 Further further JJ cord-022235-6ircruag 309 2 work work NN cord-022235-6ircruag 309 3 is be VBZ cord-022235-6ircruag 309 4 needed need VBN cord-022235-6ircruag 309 5 to to TO cord-022235-6ircruag 309 6 define define VB cord-022235-6ircruag 309 7 how how WRB cord-022235-6ircruag 309 8 proteins protein NNS cord-022235-6ircruag 309 9 find find VBP cord-022235-6ircruag 309 10 their -PRON- PRP$ cord-022235-6ircruag 309 11 way way NN cord-022235-6ircruag 309 12 to to IN cord-022235-6ircruag 309 13 budding bud VBG cord-022235-6ircruag 309 14 regions region NNS cord-022235-6ircruag 309 15 of of IN cord-022235-6ircruag 309 16 the the DT cord-022235-6ircruag 309 17 Golgi Golgi NNP cord-022235-6ircruag 309 18 membrane membrane NN cord-022235-6ircruag 309 19 and and CC cord-022235-6ircruag 309 20 what what WP cord-022235-6ircruag 309 21 triggers trigger VBZ cord-022235-6ircruag 309 22 vesicle vesicle NN cord-022235-6ircruag 309 23 formation formation NN cord-022235-6ircruag 309 24 and and CC cord-022235-6ircruag 309 25 vesicle vesicle NN cord-022235-6ircruag 309 26 fusion fusion NN cord-022235-6ircruag 309 27 with with IN cord-022235-6ircruag 309 28 acceptor acceptor NN cord-022235-6ircruag 309 29 membranes membrane NNS cord-022235-6ircruag 309 30 . . . cord-022235-6ircruag 310 1 How how WRB cord-022235-6ircruag 310 2 are be VBP cord-022235-6ircruag 310 3 proteins protein NNS cord-022235-6ircruag 310 4 specifically specifically RB cord-022235-6ircruag 310 5 retained retain VBN cord-022235-6ircruag 310 6 in in IN cord-022235-6ircruag 310 7 individual individual JJ cord-022235-6ircruag 310 8 Golgi Golgi NNP cord-022235-6ircruag 310 9 cisternae cisternae NNP cord-022235-6ircruag 310 10 ? ? . cord-022235-6ircruag 311 1 We -PRON- PRP cord-022235-6ircruag 311 2 saw see VBD cord-022235-6ircruag 311 3 earlier early RBR cord-022235-6ircruag 311 4 that that DT cord-022235-6ircruag 311 5 receptor receptor NN cord-022235-6ircruag 311 6 - - HYPH cord-022235-6ircruag 311 7 dependent dependent JJ cord-022235-6ircruag 311 8 recycling recycling NN cord-022235-6ircruag 311 9 of of IN cord-022235-6ircruag 311 10 ER ER NNP cord-022235-6ircruag 311 11 proteins protein NNS cord-022235-6ircruag 311 12 from from IN cord-022235-6ircruag 311 13 the the DT cord-022235-6ircruag 311 14 cis cis NNP cord-022235-6ircruag 311 15 Golgi Golgi NNP cord-022235-6ircruag 311 16 or or CC cord-022235-6ircruag 311 17 an an DT cord-022235-6ircruag 311 18 intermediate intermediate JJ cord-022235-6ircruag 311 19 compartment compartment NN cord-022235-6ircruag 311 20 could could MD cord-022235-6ircruag 311 21 explain explain VB cord-022235-6ircruag 311 22 how how WRB cord-022235-6ircruag 311 23 these these DT cord-022235-6ircruag 311 24 proteins protein NNS cord-022235-6ircruag 311 25 remain remain VBP cord-022235-6ircruag 311 26 almost almost RB cord-022235-6ircruag 311 27 entirely entirely RB cord-022235-6ircruag 311 28 in in IN cord-022235-6ircruag 311 29 the the DT cord-022235-6ircruag 311 30 ER ER NNP cord-022235-6ircruag 311 31 ( ( -LRB- cord-022235-6ircruag 311 32 Section section NN cord-022235-6ircruag 311 33 V.B.4 v.b.4 CD cord-022235-6ircruag 311 34 ) ) -RRB- cord-022235-6ircruag 311 35 . . . cord-022235-6ircruag 312 1 Golgi Golgi NNP cord-022235-6ircruag 312 2 residents resident NNS cord-022235-6ircruag 312 3 proba proba NNP cord-022235-6ircruag 312 4 bly bly NNP cord-022235-6ircruag 312 5 also also RB cord-022235-6ircruag 312 6 have have VBP cord-022235-6ircruag 312 7 signals signal NNS cord-022235-6ircruag 312 8 which which WDT cord-022235-6ircruag 312 9 are be VBP cord-022235-6ircruag 312 10 recognized recognize VBN cord-022235-6ircruag 312 11 by by IN cord-022235-6ircruag 312 12 some some DT cord-022235-6ircruag 312 13 kind kind NN cord-022235-6ircruag 312 14 of of IN cord-022235-6ircruag 312 15 receptor receptor NN cord-022235-6ircruag 312 16 . . . cord-022235-6ircruag 313 1 Indeed indeed RB cord-022235-6ircruag 313 2 , , , cord-022235-6ircruag 313 3 coronavirus coronavirus NN cord-022235-6ircruag 313 4 El El NNP cord-022235-6ircruag 313 5 membrane membrane NN cord-022235-6ircruag 313 6 glycoprotein glycoprotein NN cord-022235-6ircruag 313 7 appears appear VBZ cord-022235-6ircruag 313 8 to to TO cord-022235-6ircruag 313 9 have have VB cord-022235-6ircruag 313 10 a a DT cord-022235-6ircruag 313 11 Golgi Golgi NNP cord-022235-6ircruag 313 12 retention retention NN cord-022235-6ircruag 313 13 signal signal NN cord-022235-6ircruag 313 14 in in IN cord-022235-6ircruag 313 15 one one CD cord-022235-6ircruag 313 16 of of IN cord-022235-6ircruag 313 17 its -PRON- PRP$ cord-022235-6ircruag 313 18 transmembrane transmembrane NN cord-022235-6ircruag 313 19 domains domain NNS cord-022235-6ircruag 313 20 ( ( -LRB- cord-022235-6ircruag 313 21 670 670 CD cord-022235-6ircruag 313 22 ) ) -RRB- cord-022235-6ircruag 313 23 . . . cord-022235-6ircruag 314 1 However however RB cord-022235-6ircruag 314 2 , , , cord-022235-6ircruag 314 3 recycling recycling NN cord-022235-6ircruag 314 4 of of IN cord-022235-6ircruag 314 5 escaped escape VBN cord-022235-6ircruag 314 6 proteins protein NNS cord-022235-6ircruag 314 7 is be VBZ cord-022235-6ircruag 314 8 a a DT cord-022235-6ircruag 314 9 far far RB cord-022235-6ircruag 314 10 less less RBR cord-022235-6ircruag 314 11 attractive attractive JJ cord-022235-6ircruag 314 12 model model NN cord-022235-6ircruag 314 13 for for IN cord-022235-6ircruag 314 14 explaining explain VBG cord-022235-6ircruag 314 15 how how WRB cord-022235-6ircruag 314 16 Golgi Golgi NNP cord-022235-6ircruag 314 17 proteins protein NNS cord-022235-6ircruag 314 18 are be VBP cord-022235-6ircruag 314 19 retained retain VBN cord-022235-6ircruag 314 20 than than IN cord-022235-6ircruag 314 21 it -PRON- PRP cord-022235-6ircruag 314 22 is be VBZ cord-022235-6ircruag 314 23 for for IN cord-022235-6ircruag 314 24 the the DT cord-022235-6ircruag 314 25 case case NN cord-022235-6ircruag 314 26 of of IN cord-022235-6ircruag 314 27 ER ER NNP cord-022235-6ircruag 314 28 proteins protein NNS cord-022235-6ircruag 314 29 . . . cord-022235-6ircruag 315 1 One one CD cord-022235-6ircruag 315 2 possibility possibility NN cord-022235-6ircruag 315 3 , , , cord-022235-6ircruag 315 4 proposed propose VBN cord-022235-6ircruag 315 5 by by IN cord-022235-6ircruag 315 6 Pfeffer Pfeffer NNP cord-022235-6ircruag 315 7 and and CC cord-022235-6ircruag 315 8 Rothman Rothman NNP cord-022235-6ircruag 315 9 ( ( -LRB- cord-022235-6ircruag 315 10 872 872 CD cord-022235-6ircruag 315 11 ) ) -RRB- cord-022235-6ircruag 315 12 , , , cord-022235-6ircruag 315 13 is be VBZ cord-022235-6ircruag 315 14 that that IN cord-022235-6ircruag 315 15 the the DT cord-022235-6ircruag 315 16 membranes membrane NNS cord-022235-6ircruag 315 17 of of IN cord-022235-6ircruag 315 18 Golgi Golgi NNP cord-022235-6ircruag 315 19 cisternae cisternae NNP cord-022235-6ircruag 315 20 have have VBP cord-022235-6ircruag 315 21 two two CD cord-022235-6ircruag 315 22 domains domain NNS cord-022235-6ircruag 315 23 : : : cord-022235-6ircruag 315 24 a a DT cord-022235-6ircruag 315 25 fluid fluid JJ cord-022235-6ircruag 315 26 domain domain NN cord-022235-6ircruag 315 27 , , , cord-022235-6ircruag 315 28 close close JJ cord-022235-6ircruag 315 29 to to IN cord-022235-6ircruag 315 30 the the DT cord-022235-6ircruag 315 31 budding bud VBG cord-022235-6ircruag 315 32 rims rim NNS cord-022235-6ircruag 315 33 , , , cord-022235-6ircruag 315 34 and and CC cord-022235-6ircruag 315 35 an an DT cord-022235-6ircruag 315 36 immobile immobile JJ cord-022235-6ircruag 315 37 phase phase NN cord-022235-6ircruag 315 38 , , , cord-022235-6ircruag 315 39 in in IN cord-022235-6ircruag 315 40 which which WDT cord-022235-6ircruag 315 41 endogenous endogenous JJ cord-022235-6ircruag 315 42 membrane membrane NN cord-022235-6ircruag 315 43 proteins protein NNS cord-022235-6ircruag 315 44 are be VBP cord-022235-6ircruag 315 45 anchored anchor VBN cord-022235-6ircruag 315 46 . . . cord-022235-6ircruag 316 1 A a DT cord-022235-6ircruag 316 2 protein protein NN cord-022235-6ircruag 316 3 would would MD cord-022235-6ircruag 316 4 require require VB cord-022235-6ircruag 316 5 a a DT cord-022235-6ircruag 316 6 signal signal NN cord-022235-6ircruag 316 7 to to TO cord-022235-6ircruag 316 8 associate associate VB cord-022235-6ircruag 316 9 with with IN cord-022235-6ircruag 316 10 a a DT cord-022235-6ircruag 316 11 recep recep NNP cord-022235-6ircruag 316 12 tor tor NN cord-022235-6ircruag 316 13 in in IN cord-022235-6ircruag 316 14 the the DT cord-022235-6ircruag 316 15 immobile immobile JJ cord-022235-6ircruag 316 16 phase phase NN cord-022235-6ircruag 316 17 or or CC cord-022235-6ircruag 316 18 to to TO cord-022235-6ircruag 316 19 become become VB cord-022235-6ircruag 316 20 anchored anchor VBN cord-022235-6ircruag 316 21 to to IN cord-022235-6ircruag 316 22 it -PRON- PRP cord-022235-6ircruag 316 23 , , , cord-022235-6ircruag 316 24 whereas whereas IN cord-022235-6ircruag 316 25 all all DT cord-022235-6ircruag 316 26 other other JJ cord-022235-6ircruag 316 27 proteins protein NNS cord-022235-6ircruag 316 28 would would MD cord-022235-6ircruag 316 29 enter enter VB cord-022235-6ircruag 316 30 the the DT cord-022235-6ircruag 316 31 mobile mobile JJ cord-022235-6ircruag 316 32 membrane membrane NN cord-022235-6ircruag 316 33 phase phase NN cord-022235-6ircruag 316 34 or or CC cord-022235-6ircruag 316 35 the the DT cord-022235-6ircruag 316 36 bulk bulk JJ cord-022235-6ircruag 316 37 phase phase NN cord-022235-6ircruag 316 38 of of IN cord-022235-6ircruag 316 39 the the DT cord-022235-6ircruag 316 40 cisternal cisternal JJ cord-022235-6ircruag 316 41 lumen lumen NN cord-022235-6ircruag 316 42 . . . cord-022235-6ircruag 317 1 Pfeffer Pfeffer NNP cord-022235-6ircruag 317 2 and and CC cord-022235-6ircruag 317 3 Rothman Rothman NNP cord-022235-6ircruag 317 4 point point VBP cord-022235-6ircruag 317 5 out out RP cord-022235-6ircruag 317 6 that that IN cord-022235-6ircruag 317 7 this this DT cord-022235-6ircruag 317 8 model model NN cord-022235-6ircruag 317 9 reduces reduce VBZ cord-022235-6ircruag 317 10 the the DT cord-022235-6ircruag 317 11 number number NN cord-022235-6ircruag 317 12 of of IN cord-022235-6ircruag 317 13 proteins protein NNS cord-022235-6ircruag 317 14 which which WDT cord-022235-6ircruag 317 15 need need VBP cord-022235-6ircruag 317 16 to to TO cord-022235-6ircruag 317 17 have have VB cord-022235-6ircruag 317 18 signals signal NNS cord-022235-6ircruag 317 19 for for IN cord-022235-6ircruag 317 20 routing route VBG cord-022235-6ircruag 317 21 through through IN cord-022235-6ircruag 317 22 or or CC cord-022235-6ircruag 317 23 retention retention NN cord-022235-6ircruag 317 24 in in IN cord-022235-6ircruag 317 25 the the DT cord-022235-6ircruag 317 26 Golgi Golgi NNP cord-022235-6ircruag 317 27 , , , cord-022235-6ircruag 317 28 because because IN cord-022235-6ircruag 317 29 fewer few JJR cord-022235-6ircruag 317 30 proteins protein NNS cord-022235-6ircruag 317 31 are be VBP cord-022235-6ircruag 317 32 retained retain VBN cord-022235-6ircruag 317 33 in in IN cord-022235-6ircruag 317 34 the the DT cord-022235-6ircruag 317 35 Golgi Golgi NNP cord-022235-6ircruag 317 36 than than IN cord-022235-6ircruag 317 37 transit transit NN cord-022235-6ircruag 317 38 through through IN cord-022235-6ircruag 317 39 it -PRON- PRP cord-022235-6ircruag 317 40 . . . cord-022235-6ircruag 318 1 Cytoskeletal Cytoskeletal NNP cord-022235-6ircruag 318 2 structures structure NNS cord-022235-6ircruag 318 3 , , , cord-022235-6ircruag 318 4 including include VBG cord-022235-6ircruag 318 5 possibly possibly RB cord-022235-6ircruag 318 6 the the DT cord-022235-6ircruag 318 7 cytoplasmic cytoplasmic JJ cord-022235-6ircruag 318 8 matrix matrix NN cord-022235-6ircruag 318 9 , , , cord-022235-6ircruag 318 10 which which WDT cord-022235-6ircruag 318 11 " " `` cord-022235-6ircruag 318 12 glues glue VBZ cord-022235-6ircruag 318 13 " " '' cord-022235-6ircruag 318 14 the the DT cord-022235-6ircruag 318 15 cisternae cisternae NNP cord-022235-6ircruag 318 16 together together RB cord-022235-6ircruag 318 17 , , , cord-022235-6ircruag 318 18 were be VBD cord-022235-6ircruag 318 19 proposed propose VBN cord-022235-6ircruag 318 20 to to TO cord-022235-6ircruag 318 21 limit limit VB cord-022235-6ircruag 318 22 movement movement NN cord-022235-6ircruag 318 23 in in IN cord-022235-6ircruag 318 24 the the DT cord-022235-6ircruag 318 25 immobile immobile JJ cord-022235-6ircruag 318 26 phase phase NN cord-022235-6ircruag 318 27 . . . cord-022235-6ircruag 319 1 An an DT cord-022235-6ircruag 319 2 alternative alternative JJ cord-022235-6ircruag 319 3 idea idea NN cord-022235-6ircruag 319 4 , , , cord-022235-6ircruag 319 5 also also RB cord-022235-6ircruag 319 6 con con XX cord-022235-6ircruag 319 7 sidered sidere VBN cord-022235-6ircruag 319 8 by by IN cord-022235-6ircruag 319 9 Pfeffer Pfeffer NNP cord-022235-6ircruag 319 10 and and CC cord-022235-6ircruag 319 11 Rothman Rothman NNP cord-022235-6ircruag 319 12 , , , cord-022235-6ircruag 319 13 is be VBZ cord-022235-6ircruag 319 14 that that IN cord-022235-6ircruag 319 15 endogenous endogenous JJ cord-022235-6ircruag 319 16 Golgi Golgi NNP cord-022235-6ircruag 319 17 proteins protein NNS cord-022235-6ircruag 319 18 inter inter NNP cord-022235-6ircruag 319 19 act act NN cord-022235-6ircruag 319 20 to to TO cord-022235-6ircruag 319 21 form form VB cord-022235-6ircruag 319 22 patches patch NNS cord-022235-6ircruag 319 23 which which WDT cord-022235-6ircruag 319 24 , , , cord-022235-6ircruag 319 25 by by IN cord-022235-6ircruag 319 26 virtue virtue NN cord-022235-6ircruag 319 27 of of IN cord-022235-6ircruag 319 28 their -PRON- PRP$ cord-022235-6ircruag 319 29 size size NN cord-022235-6ircruag 319 30 , , , cord-022235-6ircruag 319 31 are be VBP cord-022235-6ircruag 319 32 too too RB cord-022235-6ircruag 319 33 small small JJ cord-022235-6ircruag 319 34 to to TO cord-022235-6ircruag 319 35 fit fit VB cord-022235-6ircruag 319 36 into into IN cord-022235-6ircruag 319 37 transport transport NN cord-022235-6ircruag 319 38 vesicles vesicle NNS cord-022235-6ircruag 319 39 . . . cord-022235-6ircruag 320 1 Secretory secretory JJ cord-022235-6ircruag 320 2 proteins protein NNS cord-022235-6ircruag 320 3 transit transit NN cord-022235-6ircruag 320 4 through through IN cord-022235-6ircruag 320 5 the the DT cord-022235-6ircruag 320 6 Golgi Golgi NNP cord-022235-6ircruag 320 7 apparatus apparatus NN cord-022235-6ircruag 320 8 and and CC cord-022235-6ircruag 320 9 arrive arrive VB cord-022235-6ircruag 320 10 in in IN cord-022235-6ircruag 320 11 the the DT cord-022235-6ircruag 320 12 trans trans NNP cord-022235-6ircruag 320 13 cisternae cisternae NNP cord-022235-6ircruag 320 14 together together RB cord-022235-6ircruag 320 15 . . . cord-022235-6ircruag 321 1 The the DT cord-022235-6ircruag 321 2 trans trans NNP cord-022235-6ircruag 321 3 Golgi Golgi NNP cord-022235-6ircruag 321 4 compartment compartment NN cord-022235-6ircruag 321 5 is be VBZ cord-022235-6ircruag 321 6 therefore therefore RB cord-022235-6ircruag 321 7 the the DT cord-022235-6ircruag 321 8 point point NN cord-022235-6ircruag 321 9 at at IN cord-022235-6ircruag 321 10 which which WDT cord-022235-6ircruag 321 11 the the DT cord-022235-6ircruag 321 12 different different JJ cord-022235-6ircruag 321 13 branches branch NNS cord-022235-6ircruag 321 14 of of IN cord-022235-6ircruag 321 15 the the DT cord-022235-6ircruag 321 16 secretory secretory JJ cord-022235-6ircruag 321 17 pathway pathway NN cord-022235-6ircruag 321 18 diverge diverge VBP cord-022235-6ircruag 321 19 . . . cord-022235-6ircruag 322 1 The the DT cord-022235-6ircruag 322 2 following following JJ cord-022235-6ircruag 322 3 sections section NNS cord-022235-6ircruag 322 4 deal deal VBP cord-022235-6ircruag 322 5 with with IN cord-022235-6ircruag 322 6 the the DT cord-022235-6ircruag 322 7 site site NN cord-022235-6ircruag 322 8 at at IN cord-022235-6ircruag 322 9 which which WDT cord-022235-6ircruag 322 10 sorting sorting NN cord-022235-6ircruag 322 11 occurs occur VBZ cord-022235-6ircruag 322 12 , , , cord-022235-6ircruag 322 13 the the DT cord-022235-6ircruag 322 14 ways way NNS cord-022235-6ircruag 322 15 in in IN cord-022235-6ircruag 322 16 which which WDT cord-022235-6ircruag 322 17 proteins protein NNS cord-022235-6ircruag 322 18 are be VBP cord-022235-6ircruag 322 19 sorted sort VBN cord-022235-6ircruag 322 20 , , , cord-022235-6ircruag 322 21 and and CC cord-022235-6ircruag 322 22 what what WP cord-022235-6ircruag 322 23 happens happen VBZ cord-022235-6ircruag 322 24 when when WRB cord-022235-6ircruag 322 25 vesicles vesicle NNS cord-022235-6ircruag 322 26 carrying carry VBG cord-022235-6ircruag 322 27 secretory secretory JJ cord-022235-6ircruag 322 28 proteins protein NNS cord-022235-6ircruag 322 29 arrive arrive VBP cord-022235-6ircruag 322 30 at at IN cord-022235-6ircruag 322 31 their -PRON- PRP$ cord-022235-6ircruag 322 32 destinations destination NNS cord-022235-6ircruag 322 33 . . . cord-022235-6ircruag 323 1 As as IN cord-022235-6ircruag 323 2 its -PRON- PRP$ cord-022235-6ircruag 323 3 name name NN cord-022235-6ircruag 323 4 suggests suggest VBZ cord-022235-6ircruag 323 5 , , , cord-022235-6ircruag 323 6 the the DT cord-022235-6ircruag 323 7 trans trans NNP cord-022235-6ircruag 323 8 - - NNP cord-022235-6ircruag 323 9 Golgi Golgi NNP cord-022235-6ircruag 323 10 network network NN cord-022235-6ircruag 323 11 ( ( -LRB- cord-022235-6ircruag 323 12 TGN TGN NNP cord-022235-6ircruag 323 13 , , , cord-022235-6ircruag 323 14 also also RB cord-022235-6ircruag 323 15 called call VBN cord-022235-6ircruag 323 16 Golgi Golgi NNP cord-022235-6ircruag 323 17 endoplasmic endoplasmic NN cord-022235-6ircruag 323 18 reticular reticular NN cord-022235-6ircruag 323 19 lysosomes lysosome NNS cord-022235-6ircruag 323 20 or or CC cord-022235-6ircruag 323 21 GERL gerl NN cord-022235-6ircruag 323 22 ) ) -RRB- cord-022235-6ircruag 324 1 is be VBZ cord-022235-6ircruag 324 2 the the DT cord-022235-6ircruag 324 3 most most RBS cord-022235-6ircruag 324 4 distal distal JJ cord-022235-6ircruag 324 5 compart compart NN cord-022235-6ircruag 324 6 ment ment NN cord-022235-6ircruag 324 7 of of IN cord-022235-6ircruag 324 8 the the DT cord-022235-6ircruag 324 9 Golgi Golgi NNP cord-022235-6ircruag 324 10 apparatus apparatus NN cord-022235-6ircruag 324 11 ( ( -LRB- cord-022235-6ircruag 324 12 relative relative JJ cord-022235-6ircruag 324 13 to to IN cord-022235-6ircruag 324 14 the the DT cord-022235-6ircruag 324 15 RER RER NNP cord-022235-6ircruag 324 16 ) ) -RRB- cord-022235-6ircruag 324 17 . . . cord-022235-6ircruag 325 1 It -PRON- PRP cord-022235-6ircruag 325 2 differs differ VBZ cord-022235-6ircruag 325 3 from from IN cord-022235-6ircruag 325 4 the the DT cord-022235-6ircruag 325 5 Golgi Golgi NNP cord-022235-6ircruag 325 6 cisternae cisternae NNP cord-022235-6ircruag 325 7 in in IN cord-022235-6ircruag 325 8 that that IN cord-022235-6ircruag 325 9 it -PRON- PRP cord-022235-6ircruag 325 10 has have VBZ cord-022235-6ircruag 325 11 a a DT cord-022235-6ircruag 325 12 distended distended JJ cord-022235-6ircruag 325 13 , , , cord-022235-6ircruag 325 14 reticular reticular JJ cord-022235-6ircruag 325 15 appearance appearance NN cord-022235-6ircruag 325 16 rather rather RB cord-022235-6ircruag 325 17 than than IN cord-022235-6ircruag 325 18 that that DT cord-022235-6ircruag 325 19 of of IN cord-022235-6ircruag 325 20 a a DT cord-022235-6ircruag 325 21 flattened flatten VBN cord-022235-6ircruag 325 22 dish dish NN cord-022235-6ircruag 325 23 . . . cord-022235-6ircruag 326 1 Early early JJ cord-022235-6ircruag 326 2 morphological morphological JJ cord-022235-6ircruag 326 3 studies study NNS cord-022235-6ircruag 326 4 suggested suggest VBD cord-022235-6ircruag 326 5 that that IN cord-022235-6ircruag 326 6 the the DT cord-022235-6ircruag 326 7 TGN TGN NNP cord-022235-6ircruag 326 8 was be VBD cord-022235-6ircruag 326 9 a a DT cord-022235-6ircruag 326 10 reticular reticular JJ cord-022235-6ircruag 326 11 adjunct adjunct NN cord-022235-6ircruag 326 12 of of IN cord-022235-6ircruag 326 13 the the DT cord-022235-6ircruag 326 14 Golgi Golgi NNP cord-022235-6ircruag 326 15 specifically specifically RB cord-022235-6ircruag 326 16 involved involve VBN cord-022235-6ircruag 326 17 in in IN cord-022235-6ircruag 326 18 lysosome lysosome NN cord-022235-6ircruag 326 19 biosynthesis biosynthesis NN cord-022235-6ircruag 326 20 [ [ -LRB- cord-022235-6ircruag 326 21 lysosomal lysosomal JJ cord-022235-6ircruag 326 22 enzymes enzyme NNS cord-022235-6ircruag 326 23 were be VBD cord-022235-6ircruag 326 24 originally originally RB cord-022235-6ircruag 326 25 thought think VBN cord-022235-6ircruag 326 26 to to TO cord-022235-6ircruag 326 27 bypass bypass VB cord-022235-6ircruag 326 28 the the DT cord-022235-6ircruag 326 29 Golgi Golgi NNP cord-022235-6ircruag 326 30 ( ( -LRB- cord-022235-6ircruag 326 31 398 398 CD cord-022235-6ircruag 326 32 ) ) -RRB- cord-022235-6ircruag 326 33 ] ] -RRB- cord-022235-6ircruag 326 34 . . . cord-022235-6ircruag 327 1 The the DT cord-022235-6ircruag 327 2 TGN TGN NNP cord-022235-6ircruag 327 3 is be VBZ cord-022235-6ircruag 327 4 now now RB cord-022235-6ircruag 327 5 known know VBN cord-022235-6ircruag 327 6 to to TO cord-022235-6ircruag 327 7 be be VB cord-022235-6ircruag 327 8 distinct distinct JJ cord-022235-6ircruag 327 9 from from IN cord-022235-6ircruag 327 10 endosomes endosome NNS cord-022235-6ircruag 327 11 or or CC cord-022235-6ircruag 327 12 lysosomes lysosome NNS cord-022235-6ircruag 327 13 . . . cord-022235-6ircruag 328 1 Endocytosed endocytosed JJ cord-022235-6ircruag 328 2 horseradish horseradish NN cord-022235-6ircruag 328 3 peroxidase peroxidase NN cord-022235-6ircruag 328 4 does do VBZ cord-022235-6ircruag 328 5 not not RB cord-022235-6ircruag 328 6 accumulate accumulate VB cord-022235-6ircruag 328 7 in in IN cord-022235-6ircruag 328 8 the the DT cord-022235-6ircruag 328 9 TGN TGN NNP cord-022235-6ircruag 328 10 ( ( -LRB- cord-022235-6ircruag 328 11 401 401 CD cord-022235-6ircruag 328 12 ) ) -RRB- cord-022235-6ircruag 328 13 , , , cord-022235-6ircruag 328 14 although although IN cord-022235-6ircruag 328 15 some some DT cord-022235-6ircruag 328 16 endocytosed endocytose VBN cord-022235-6ircruag 328 17 proteins protein NNS cord-022235-6ircruag 328 18 may may MD cord-022235-6ircruag 328 19 be be VB cord-022235-6ircruag 328 20 recycled recycle VBN cord-022235-6ircruag 328 21 to to IN cord-022235-6ircruag 328 22 the the DT cord-022235-6ircruag 328 23 cell cell NN cord-022235-6ircruag 328 24 surface surface NN cord-022235-6ircruag 328 25 via via IN cord-022235-6ircruag 328 26 the the DT cord-022235-6ircruag 328 27 trans trans NNP cord-022235-6ircruag 328 28 Golgi Golgi NNP cord-022235-6ircruag 328 29 and and CC cord-022235-6ircruag 328 30 especially especially RB cord-022235-6ircruag 328 31 via via IN cord-022235-6ircruag 328 32 the the DT cord-022235-6ircruag 328 33 TGN TGN NNP cord-022235-6ircruag 328 34 ( ( -LRB- cord-022235-6ircruag 328 35 319 319 CD cord-022235-6ircruag 328 36 ) ) -RRB- cord-022235-6ircruag 328 37 ( ( -LRB- cord-022235-6ircruag 328 38 see see VB cord-022235-6ircruag 328 39 Chapter chapter NN cord-022235-6ircruag 328 40 VIII VIII NNP cord-022235-6ircruag 328 41 ) ) -RRB- cord-022235-6ircruag 328 42 . . . cord-022235-6ircruag 329 1 The the DT cord-022235-6ircruag 329 2 TGN TGN NNP cord-022235-6ircruag 329 3 probably probably RB cord-022235-6ircruag 329 4 contains contain VBZ cord-022235-6ircruag 329 5 tyrosinyl tyrosinyl NNP cord-022235-6ircruag 329 6 sulfotransferase sulfotransferase NNP cord-022235-6ircruag 329 7 ( ( -LRB- cord-022235-6ircruag 329 8 33 33 CD cord-022235-6ircruag 329 9 ) ) -RRB- cord-022235-6ircruag 329 10 , , , cord-022235-6ircruag 329 11 acid acid JJ cord-022235-6ircruag 329 12 phosphatase phosphatase NN cord-022235-6ircruag 329 13 , , , cord-022235-6ircruag 329 14 sialotransferase sialotransferase NNP cord-022235-6ircruag 329 15 , , , cord-022235-6ircruag 329 16 and and CC cord-022235-6ircruag 329 17 galactosyltransferase galactosyltransferase NNP cord-022235-6ircruag 329 18 ( ( -LRB- cord-022235-6ircruag 329 19 365,957 365,957 CD cord-022235-6ircruag 329 20 ) ) -RRB- cord-022235-6ircruag 330 1 ( ( -LRB- cord-022235-6ircruag 330 2 Table table NN cord-022235-6ircruag 330 3 V V NNP cord-022235-6ircruag 330 4 .l .l NNP cord-022235-6ircruag 330 5 , , , cord-022235-6ircruag 331 1 Fig fig NN cord-022235-6ircruag 331 2 . . . cord-022235-6ircruag 331 3 V.3 V.3 NNP cord-022235-6ircruag 331 4 ) ) -RRB- cord-022235-6ircruag 331 5 , , , cord-022235-6ircruag 331 6 although although IN cord-022235-6ircruag 331 7 TGN TGN NNP cord-022235-6ircruag 331 8 - - HYPH cord-022235-6ircruag 331 9 derived derive VBN cord-022235-6ircruag 331 10 vesicles vesicle NNS cord-022235-6ircruag 331 11 are be VBP cord-022235-6ircruag 331 12 diffi diffi JJ cord-022235-6ircruag 331 13 cult cult NN cord-022235-6ircruag 331 14 to to TO cord-022235-6ircruag 331 15 distinguish distinguish VB cord-022235-6ircruag 331 16 from from IN cord-022235-6ircruag 331 17 those those DT cord-022235-6ircruag 331 18 derived derive VBN cord-022235-6ircruag 331 19 from from IN cord-022235-6ircruag 331 20 the the DT cord-022235-6ircruag 331 21 trans trans NNP cord-022235-6ircruag 331 22 cisternae cisternae NNP cord-022235-6ircruag 331 23 . . . cord-022235-6ircruag 332 1 The the DT cord-022235-6ircruag 332 2 TGN TGN NNP cord-022235-6ircruag 332 3 also also RB cord-022235-6ircruag 332 4 marks mark VBZ cord-022235-6ircruag 332 5 the the DT cord-022235-6ircruag 332 6 site site NN cord-022235-6ircruag 332 7 at at IN cord-022235-6ircruag 332 8 which which WDT cord-022235-6ircruag 332 9 assembled assemble VBD cord-022235-6ircruag 332 10 clathrin clathrin NN cord-022235-6ircruag 332 11 , , , cord-022235-6ircruag 332 12 one one CD cord-022235-6ircruag 332 13 of of IN cord-022235-6ircruag 332 14 the the DT cord-022235-6ircruag 332 15 proteins protein NNS cord-022235-6ircruag 332 16 which which WDT cord-022235-6ircruag 332 17 coat coat VBP cord-022235-6ircruag 332 18 some some DT cord-022235-6ircruag 332 19 secretory secretory JJ cord-022235-6ircruag 332 20 and and CC cord-022235-6ircruag 332 21 endocytic endocytic JJ cord-022235-6ircruag 332 22 vesicles vesicle NNS cord-022235-6ircruag 332 23 , , , cord-022235-6ircruag 332 24 appears appear VBZ cord-022235-6ircruag 332 25 along along IN cord-022235-6ircruag 332 26 the the DT cord-022235-6ircruag 332 27 secretory secretory JJ cord-022235-6ircruag 332 28 pathway pathway NN cord-022235-6ircruag 332 29 ( ( -LRB- cord-022235-6ircruag 332 30 see see VB cord-022235-6ircruag 332 31 Section section NN cord-022235-6ircruag 332 32 V.G.6 V.G.6 NNP cord-022235-6ircruag 332 33 ) ) -RRB- cord-022235-6ircruag 332 34 . . . cord-022235-6ircruag 333 1 The the DT cord-022235-6ircruag 333 2 TGN TGN NNP cord-022235-6ircruag 333 3 is be VBZ cord-022235-6ircruag 333 4 the the DT cord-022235-6ircruag 333 5 most most RBS cord-022235-6ircruag 333 6 acidic acidic JJ cord-022235-6ircruag 333 7 Golgi Golgi NNP cord-022235-6ircruag 333 8 compartment compartment NN cord-022235-6ircruag 333 9 , , , cord-022235-6ircruag 333 10 although although IN cord-022235-6ircruag 333 11 the the DT cord-022235-6ircruag 333 12 pH pH NNP cord-022235-6ircruag 333 13 is be VBZ cord-022235-6ircruag 333 14 almost almost RB cord-022235-6ircruag 333 15 certainly certainly RB cord-022235-6ircruag 333 16 not not RB cord-022235-6ircruag 333 17 as as RB cord-022235-6ircruag 333 18 low low JJ cord-022235-6ircruag 333 19 as as IN cord-022235-6ircruag 333 20 in in IN cord-022235-6ircruag 333 21 secretory secretory JJ cord-022235-6ircruag 333 22 granules granule NNS cord-022235-6ircruag 333 23 ( ( -LRB- cord-022235-6ircruag 333 24 823 823 CD cord-022235-6ircruag 333 25 ) ) -RRB- cord-022235-6ircruag 333 26 or or CC cord-022235-6ircruag 333 27 in in IN cord-022235-6ircruag 333 28 lysosomal lysosomal JJ cord-022235-6ircruag 333 29 sorting sorting NN cord-022235-6ircruag 333 30 vesicles vesicle NNS cord-022235-6ircruag 333 31 , , , cord-022235-6ircruag 333 32 in in IN cord-022235-6ircruag 333 33 which which WDT cord-022235-6ircruag 333 34 low low JJ cord-022235-6ircruag 333 35 pH pH NNP cord-022235-6ircruag 333 36 causes cause VBZ cord-022235-6ircruag 333 37 the the DT cord-022235-6ircruag 333 38 dissociation dissociation NN cord-022235-6ircruag 333 39 of of IN cord-022235-6ircruag 333 40 lysosomal lysosomal JJ cord-022235-6ircruag 333 41 proteins protein NNS cord-022235-6ircruag 333 42 from from IN cord-022235-6ircruag 333 43 the the DT cord-022235-6ircruag 333 44 mannose-6-phosphate mannose-6-phosphate NNP cord-022235-6ircruag 333 45 receptor receptor NN cord-022235-6ircruag 333 46 ( ( -LRB- cord-022235-6ircruag 333 47 577 577 CD cord-022235-6ircruag 333 48 ) ) -RRB- cord-022235-6ircruag 333 49 ( ( -LRB- cord-022235-6ircruag 333 50 see see VB cord-022235-6ircruag 333 51 Section section NN cord-022235-6ircruag 333 52 V.G.5 V.G.5 NNP cord-022235-6ircruag 333 53 ) ) -RRB- cord-022235-6ircruag 333 54 . . . cord-022235-6ircruag 334 1 Furthermore furthermore RB cord-022235-6ircruag 334 2 , , , cord-022235-6ircruag 334 3 influenza influenza NN cord-022235-6ircruag 334 4 virus virus NN cord-022235-6ircruag 334 5 hemagglutinin hemagglutinin NN cord-022235-6ircruag 334 6 , , , cord-022235-6ircruag 334 7 which which WDT cord-022235-6ircruag 334 8 is be VBZ cord-022235-6ircruag 334 9 activated activate VBN cord-022235-6ircruag 334 10 at at IN cord-022235-6ircruag 334 11 low low JJ cord-022235-6ircruag 334 12 pH ph NN cord-022235-6ircruag 334 13 , , , cord-022235-6ircruag 334 14 reaches reach VBZ cord-022235-6ircruag 334 15 the the DT cord-022235-6ircruag 334 16 cell cell NN cord-022235-6ircruag 334 17 surface surface NN cord-022235-6ircruag 334 18 as as IN cord-022235-6ircruag 334 19 a a DT cord-022235-6ircruag 334 20 nonactivated nonactivated JJ cord-022235-6ircruag 334 21 form form NN cord-022235-6ircruag 334 22 , , , cord-022235-6ircruag 334 23 indicating indicate VBG cord-022235-6ircruag 334 24 that that IN cord-022235-6ircruag 334 25 it -PRON- PRP cord-022235-6ircruag 334 26 does do VBZ cord-022235-6ircruag 334 27 not not RB cord-022235-6ircruag 334 28 spend spend VB cord-022235-6ircruag 334 29 an an DT cord-022235-6ircruag 334 30 appreciable appreciable JJ cord-022235-6ircruag 334 31 period period NN cord-022235-6ircruag 334 32 ( ( -LRB- cord-022235-6ircruag 334 33 more more JJR cord-022235-6ircruag 334 34 than than IN cord-022235-6ircruag 334 35 2 2 CD cord-022235-6ircruag 334 36 min min NN cord-022235-6ircruag 334 37 ) ) -RRB- cord-022235-6ircruag 334 38 in in IN cord-022235-6ircruag 334 39 a a DT cord-022235-6ircruag 334 40 compartment compartment NN cord-022235-6ircruag 334 41 with with IN cord-022235-6ircruag 334 42 a a DT cord-022235-6ircruag 334 43 pH ph NN cord-022235-6ircruag 334 44 of of IN cord-022235-6ircruag 334 45 less less JJR cord-022235-6ircruag 334 46 than than IN cord-022235-6ircruag 334 47 6 6 CD cord-022235-6ircruag 334 48 ( ( -LRB- cord-022235-6ircruag 334 49 107 107 CD cord-022235-6ircruag 334 50 ) ) -RRB- cord-022235-6ircruag 334 51 . . . cord-022235-6ircruag 335 1 Different different JJ cord-022235-6ircruag 335 2 secretory secretory JJ cord-022235-6ircruag 335 3 proteins protein NNS cord-022235-6ircruag 335 4 accumulate accumulate VBP cord-022235-6ircruag 335 5 together together RB cord-022235-6ircruag 335 6 in in IN cord-022235-6ircruag 335 7 the the DT cord-022235-6ircruag 335 8 TGN TGN NNP cord-022235-6ircruag 335 9 ( ( -LRB- cord-022235-6ircruag 335 10 1088,1123 1088,1123 CD cord-022235-6ircruag 335 11 ) ) -RRB- cord-022235-6ircruag 335 12 , , , cord-022235-6ircruag 335 13 which which WDT cord-022235-6ircruag 335 14 can can MD cord-022235-6ircruag 335 15 become become VB cord-022235-6ircruag 335 16 further far RBR cord-022235-6ircruag 335 17 distended distend VBN cord-022235-6ircruag 335 18 when when WRB cord-022235-6ircruag 335 19 the the DT cord-022235-6ircruag 335 20 load load NN cord-022235-6ircruag 335 21 of of IN cord-022235-6ircruag 335 22 secre secre NNP cord-022235-6ircruag 335 23 tory tory NNP cord-022235-6ircruag 335 24 or or CC cord-022235-6ircruag 335 25 lysosomal lysosomal JJ cord-022235-6ircruag 335 26 proteins protein NNS cord-022235-6ircruag 335 27 increases increase NNS cord-022235-6ircruag 335 28 ( ( -LRB- cord-022235-6ircruag 335 29 398 398 CD cord-022235-6ircruag 335 30 ) ) -RRB- cord-022235-6ircruag 335 31 . . . cord-022235-6ircruag 336 1 Transport transport NN cord-022235-6ircruag 336 2 of of IN cord-022235-6ircruag 336 3 secretory secretory JJ cord-022235-6ircruag 336 4 pro pro FW cord-022235-6ircruag 336 5 teins tein NNS cord-022235-6ircruag 336 6 from from IN cord-022235-6ircruag 336 7 the the DT cord-022235-6ircruag 336 8 TGN TGN NNP cord-022235-6ircruag 336 9 is be VBZ cord-022235-6ircruag 336 10 blocked block VBN cord-022235-6ircruag 336 11 at at IN cord-022235-6ircruag 336 12 20 20 CD cord-022235-6ircruag 336 13 ° ° NN cord-022235-6ircruag 336 14 C c NN cord-022235-6ircruag 336 15 , , , cord-022235-6ircruag 336 16 and and CC cord-022235-6ircruag 336 17 numerous numerous JJ cord-022235-6ircruag 336 18 protein protein NN cord-022235-6ircruag 336 19 - - HYPH cord-022235-6ircruag 336 20 coated coat VBN cord-022235-6ircruag 336 21 and and CC cord-022235-6ircruag 336 22 naked naked JJ cord-022235-6ircruag 336 23 vesicles vesicle NNS cord-022235-6ircruag 336 24 accumulate accumulate VBP cord-022235-6ircruag 336 25 as as IN cord-022235-6ircruag 336 26 buds bud NNS cord-022235-6ircruag 336 27 on on IN cord-022235-6ircruag 336 28 the the DT cord-022235-6ircruag 336 29 surface surface NN cord-022235-6ircruag 336 30 of of IN cord-022235-6ircruag 336 31 the the DT cord-022235-6ircruag 336 32 TGN TGN NNP cord-022235-6ircruag 336 33 ( ( -LRB- cord-022235-6ircruag 336 34 401 401 CD cord-022235-6ircruag 336 35 ) ) -RRB- cord-022235-6ircruag 336 36 . . . cord-022235-6ircruag 337 1 Different different JJ cord-022235-6ircruag 337 2 types type NNS cord-022235-6ircruag 337 3 of of IN cord-022235-6ircruag 337 4 vesicles vesicle NNS cord-022235-6ircruag 337 5 seem seem VBP cord-022235-6ircruag 337 6 to to TO cord-022235-6ircruag 337 7 be be VB cord-022235-6ircruag 337 8 involved involve VBN cord-022235-6ircruag 337 9 in in IN cord-022235-6ircruag 337 10 sorting sort VBG cord-022235-6ircruag 337 11 secretory secretory JJ cord-022235-6ircruag 337 12 pro pro FW cord-022235-6ircruag 337 13 teins tein NNS cord-022235-6ircruag 337 14 into into IN cord-022235-6ircruag 337 15 different different JJ cord-022235-6ircruag 337 16 branches branch NNS cord-022235-6ircruag 337 17 of of IN cord-022235-6ircruag 337 18 the the DT cord-022235-6ircruag 337 19 secretory secretory JJ cord-022235-6ircruag 337 20 pathway pathway NN cord-022235-6ircruag 337 21 . . . cord-022235-6ircruag 338 1 Thus thus RB cord-022235-6ircruag 338 2 , , , cord-022235-6ircruag 338 3 different different JJ cord-022235-6ircruag 338 4 classes class NNS cord-022235-6ircruag 338 5 of of IN cord-022235-6ircruag 338 6 soluble soluble JJ cord-022235-6ircruag 338 7 secretory secretory JJ cord-022235-6ircruag 338 8 proteins protein NNS cord-022235-6ircruag 338 9 may may MD cord-022235-6ircruag 338 10 be be VB cord-022235-6ircruag 338 11 segregated segregate VBN cord-022235-6ircruag 338 12 into into IN cord-022235-6ircruag 338 13 different different JJ cord-022235-6ircruag 338 14 domains domain NNS cord-022235-6ircruag 338 15 of of IN cord-022235-6ircruag 338 16 the the DT cord-022235-6ircruag 338 17 TGN TGN NNP cord-022235-6ircruag 338 18 according accord VBG cord-022235-6ircruag 338 19 to to IN cord-022235-6ircruag 338 20 their -PRON- PRP$ cord-022235-6ircruag 338 21 interaction interaction NN cord-022235-6ircruag 338 22 with with IN cord-022235-6ircruag 338 23 specific specific JJ cord-022235-6ircruag 338 24 receptors receptor NNS cord-022235-6ircruag 338 25 ( ( -LRB- cord-022235-6ircruag 338 26 Fig fig NN cord-022235-6ircruag 338 27 . . . cord-022235-6ircruag 338 28 V.4 V.4 NNP cord-022235-6ircruag 338 29 ) ) -RRB- cord-022235-6ircruag 338 30 . . . cord-022235-6ircruag 339 1 Although although IN cord-022235-6ircruag 339 2 this this DT cord-022235-6ircruag 339 3 model model NN cord-022235-6ircruag 339 4 does do VBZ cord-022235-6ircruag 339 5 not not RB cord-022235-6ircruag 339 6 explain explain VB cord-022235-6ircruag 339 7 how how WRB cord-022235-6ircruag 339 8 receptor receptor NN cord-022235-6ircruag 339 9 proteins protein NNS cord-022235-6ircruag 339 10 Note note VBP cord-022235-6ircruag 339 11 that that IN cord-022235-6ircruag 339 12 constitutive constitutive JJ cord-022235-6ircruag 339 13 default default NN cord-022235-6ircruag 339 14 sorting sorting NN cord-022235-6ircruag 339 15 is be VBZ cord-022235-6ircruag 339 16 not not RB cord-022235-6ircruag 339 17 receptor receptor NN cord-022235-6ircruag 339 18 - - HYPH cord-022235-6ircruag 339 19 mediated mediate VBN cord-022235-6ircruag 339 20 and and CC cord-022235-6ircruag 339 21 that that IN cord-022235-6ircruag 339 22 sorting sort VBG cord-022235-6ircruag 339 23 into into IN cord-022235-6ircruag 339 24 the the DT cord-022235-6ircruag 339 25 regulated regulate VBN cord-022235-6ircruag 339 26 secretory secretory JJ cord-022235-6ircruag 339 27 branch branch NN cord-022235-6ircruag 339 28 of of IN cord-022235-6ircruag 339 29 the the DT cord-022235-6ircruag 339 30 pathway pathway NN cord-022235-6ircruag 339 31 may may MD cord-022235-6ircruag 339 32 result result VB cord-022235-6ircruag 339 33 from from IN cord-022235-6ircruag 339 34 protein protein NN cord-022235-6ircruag 339 35 aggregation aggregation NN cord-022235-6ircruag 339 36 and and CC cord-022235-6ircruag 339 37 granule granule NN cord-022235-6ircruag 339 38 formation formation NN cord-022235-6ircruag 339 39 rather rather RB cord-022235-6ircruag 339 40 than than IN cord-022235-6ircruag 339 41 receptor receptor NN cord-022235-6ircruag 339 42 interactions interaction NNS cord-022235-6ircruag 339 43 . . . cord-022235-6ircruag 340 1 TGN TGN NNP cord-022235-6ircruag 340 2 , , , cord-022235-6ircruag 340 3 trans trans NNP cord-022235-6ircruag 340 4 - - NNP cord-022235-6ircruag 340 5 Golgi Golgi NNP cord-022235-6ircruag 340 6 network network NN cord-022235-6ircruag 340 7 ; ; : cord-022235-6ircruag 340 8 L l NN cord-022235-6ircruag 340 9 , , , cord-022235-6ircruag 340 10 lysosome lysosome NN cord-022235-6ircruag 340 11 ; ; : cord-022235-6ircruag 340 12 PL pl NN cord-022235-6ircruag 340 13 , , , cord-022235-6ircruag 340 14 prelysosome prelysosome NNP cord-022235-6ircruag 340 15 . . . cord-022235-6ircruag 341 1 segregate segregate VB cord-022235-6ircruag 341 2 into into IN cord-022235-6ircruag 341 3 these these DT cord-022235-6ircruag 341 4 domains domain NNS cord-022235-6ircruag 341 5 , , , cord-022235-6ircruag 341 6 it -PRON- PRP cord-022235-6ircruag 341 7 does do VBZ cord-022235-6ircruag 341 8 serve serve VB cord-022235-6ircruag 341 9 to to TO cord-022235-6ircruag 341 10 illustrate illustrate VB cord-022235-6ircruag 341 11 the the DT cord-022235-6ircruag 341 12 possible possible JJ cord-022235-6ircruag 341 13 mech mech NN cord-022235-6ircruag 341 14 anisms anism NNS cord-022235-6ircruag 341 15 involved involve VBN cord-022235-6ircruag 341 16 in in IN cord-022235-6ircruag 341 17 receptor receptor NN cord-022235-6ircruag 341 18 - - HYPH cord-022235-6ircruag 341 19 dependent dependent JJ cord-022235-6ircruag 341 20 segregation segregation NN cord-022235-6ircruag 341 21 and and CC cord-022235-6ircruag 341 22 packaging packaging NN cord-022235-6ircruag 341 23 secre secre NNP cord-022235-6ircruag 341 24 tory tory NNP cord-022235-6ircruag 341 25 proteins protein NNS cord-022235-6ircruag 341 26 discussed discuss VBN cord-022235-6ircruag 341 27 in in IN cord-022235-6ircruag 341 28 the the DT cord-022235-6ircruag 341 29 following follow VBG cord-022235-6ircruag 341 30 sections section NNS cord-022235-6ircruag 341 31 . . . cord-022235-6ircruag 342 1 Default default NN cord-022235-6ircruag 342 2 sorting sorting NN cord-022235-6ircruag 342 3 is be VBZ cord-022235-6ircruag 342 4 the the DT cord-022235-6ircruag 342 5 final final JJ cord-022235-6ircruag 342 6 stage stage NN cord-022235-6ircruag 342 7 in in IN cord-022235-6ircruag 342 8 the the DT cord-022235-6ircruag 342 9 secretion secretion NN cord-022235-6ircruag 342 10 of of IN cord-022235-6ircruag 342 11 proteins protein NNS cord-022235-6ircruag 342 12 which which WDT cord-022235-6ircruag 342 13 lack lack VBP cord-022235-6ircruag 342 14 specific specific JJ cord-022235-6ircruag 342 15 sorting sorting NN cord-022235-6ircruag 342 16 ( ( -LRB- cord-022235-6ircruag 342 17 lysosomal lysosomal NN cord-022235-6ircruag 342 18 , , , cord-022235-6ircruag 342 19 vacuolar vacuolar NNP cord-022235-6ircruag 342 20 , , , cord-022235-6ircruag 342 21 or or CC cord-022235-6ircruag 342 22 polarity polarity NN cord-022235-6ircruag 342 23 ) ) -RRB- cord-022235-6ircruag 342 24 signals signal NNS cord-022235-6ircruag 342 25 and and CC cord-022235-6ircruag 342 26 which which WDT cord-022235-6ircruag 342 27 are be VBP cord-022235-6ircruag 342 28 not not RB cord-022235-6ircruag 342 29 accumulated accumulate VBN cord-022235-6ircruag 342 30 within within IN cord-022235-6ircruag 342 31 specific specific JJ cord-022235-6ircruag 342 32 secretory secretory JJ cord-022235-6ircruag 342 33 storage storage NN cord-022235-6ircruag 342 34 granules granule NNS cord-022235-6ircruag 342 35 of of IN cord-022235-6ircruag 342 36 the the DT cord-022235-6ircruag 342 37 regu regu NN cord-022235-6ircruag 342 38 lated late VBN cord-022235-6ircruag 342 39 branch branch NN cord-022235-6ircruag 342 40 of of IN cord-022235-6ircruag 342 41 the the DT cord-022235-6ircruag 342 42 secretory secretory JJ cord-022235-6ircruag 342 43 pathway pathway NN cord-022235-6ircruag 342 44 , , , cord-022235-6ircruag 342 45 i.e. i.e. FW cord-022235-6ircruag 342 46 , , , cord-022235-6ircruag 342 47 those those DT cord-022235-6ircruag 342 48 which which WDT cord-022235-6ircruag 342 49 are be VBP cord-022235-6ircruag 342 50 constitutively constitutively RB cord-022235-6ircruag 342 51 secreted secrete VBN cord-022235-6ircruag 342 52 ( ( -LRB- cord-022235-6ircruag 342 53 Fig fig NN cord-022235-6ircruag 342 54 . . . cord-022235-6ircruag 342 55 V.4 V.4 NNP cord-022235-6ircruag 342 56 ) ) -RRB- cord-022235-6ircruag 342 57 . . . cord-022235-6ircruag 343 1 ( ( -LRB- cord-022235-6ircruag 343 2 Note note VB cord-022235-6ircruag 343 3 , , , cord-022235-6ircruag 343 4 however however RB cord-022235-6ircruag 343 5 , , , cord-022235-6ircruag 343 6 that that IN cord-022235-6ircruag 343 7 some some DT cord-022235-6ircruag 343 8 constitutively constitutively RB cord-022235-6ircruag 343 9 secreted secrete VBN cord-022235-6ircruag 343 10 proteins protein NNS cord-022235-6ircruag 343 11 carry carry VBP cord-022235-6ircruag 343 12 sorting sorting NN cord-022235-6ircruag 343 13 signals signal NNS cord-022235-6ircruag 343 14 . . . cord-022235-6ircruag 343 15 ) ) -RRB- cord-022235-6ircruag 344 1 Immunocytochemical Immunocytochemical NNP cord-022235-6ircruag 344 2 studies study NNS cord-022235-6ircruag 344 3 show show VBP cord-022235-6ircruag 344 4 that that IN cord-022235-6ircruag 344 5 proteins protein NNS cord-022235-6ircruag 344 6 secreted secrete VBN cord-022235-6ircruag 344 7 by by IN cord-022235-6ircruag 344 8 the the DT cord-022235-6ircruag 344 9 default default NN cord-022235-6ircruag 344 10 pathway pathway NN cord-022235-6ircruag 344 11 accumulate accumulate VBP cord-022235-6ircruag 344 12 in in IN cord-022235-6ircruag 344 13 secretory secretory JJ cord-022235-6ircruag 344 14 vesicles vesicle NNS cord-022235-6ircruag 344 15 which which WDT cord-022235-6ircruag 344 16 do do VBP cord-022235-6ircruag 344 17 not not RB cord-022235-6ircruag 344 18 have have VB cord-022235-6ircruag 344 19 protein protein NN cord-022235-6ircruag 344 20 coats coat NNS cord-022235-6ircruag 344 21 ( ( -LRB- cord-022235-6ircruag 344 22 401,821 401,821 CD cord-022235-6ircruag 344 23 ) ) -RRB- cord-022235-6ircruag 344 24 . . . cord-022235-6ircruag 345 1 Plasma plasma NN cord-022235-6ircruag 345 2 membrane membrane NN cord-022235-6ircruag 345 3 proteins protein NNS cord-022235-6ircruag 345 4 are be VBP cord-022235-6ircruag 345 5 transported transport VBN cord-022235-6ircruag 345 6 to to IN cord-022235-6ircruag 345 7 the the DT cord-022235-6ircruag 345 8 cell cell NN cord-022235-6ircruag 345 9 surface surface NN cord-022235-6ircruag 345 10 in in IN cord-022235-6ircruag 345 11 the the DT cord-022235-6ircruag 345 12 same same JJ cord-022235-6ircruag 345 13 vesicles vesicle NNS cord-022235-6ircruag 345 14 as as IN cord-022235-6ircruag 345 15 secreted secrete VBN cord-022235-6ircruag 345 16 proteins protein NNS cord-022235-6ircruag 345 17 ( ( -LRB- cord-022235-6ircruag 345 18 114 114 CD cord-022235-6ircruag 345 19 , , , cord-022235-6ircruag 345 20 462 462 CD cord-022235-6ircruag 345 21 , , , cord-022235-6ircruag 345 22 1088 1088 CD cord-022235-6ircruag 345 23 ) ) -RRB- cord-022235-6ircruag 345 24 . . . cord-022235-6ircruag 346 1 ( ( -LRB- cord-022235-6ircruag 346 2 1204 1204 CD cord-022235-6ircruag 346 3 ) ) -RRB- cord-022235-6ircruag 347 1 ( ( -LRB- cord-022235-6ircruag 347 2 see see VB cord-022235-6ircruag 347 3 Section Section NNP cord-022235-6ircruag 347 4 II II NNP cord-022235-6ircruag 347 5 . . . cord-022235-6ircruag 348 1 F. F. NNP cord-022235-6ircruag 348 2 3 3 CD cord-022235-6ircruag 348 3 ) ) -RRB- cord-022235-6ircruag 348 4 required require VBD cord-022235-6ircruag 348 5 ATP ATP NNP cord-022235-6ircruag 348 6 and and CC cord-022235-6ircruag 348 7 was be VBD cord-022235-6ircruag 348 8 sensitive sensitive JJ cord-022235-6ircruag 348 9 to to IN cord-022235-6ircruag 348 10 trypsin trypsin NN cord-022235-6ircruag 348 11 , , , cord-022235-6ircruag 348 12 implying imply VBG cord-022235-6ircruag 348 13 that that IN cord-022235-6ircruag 348 14 at at RB cord-022235-6ircruag 348 15 least least RBS cord-022235-6ircruag 348 16 one one CD cord-022235-6ircruag 348 17 cytoplasmic cytoplasmic JJ cord-022235-6ircruag 348 18 or or CC cord-022235-6ircruag 348 19 vesicle vesicle NN cord-022235-6ircruag 348 20 - - HYPH cord-022235-6ircruag 348 21 cytoplasmic cytoplasmic JJ cord-022235-6ircruag 348 22 membrane membrane NN cord-022235-6ircruag 348 23 surface surface NN cord-022235-6ircruag 348 24 protein protein NN cord-022235-6ircruag 348 25 is be VBZ cord-022235-6ircruag 348 26 involved involve VBN cord-022235-6ircruag 348 27 . . . cord-022235-6ircruag 349 1 ATP ATP NNP cord-022235-6ircruag 349 2 might may MD cord-022235-6ircruag 349 3 be be VB cord-022235-6ircruag 349 4 needed need VBN cord-022235-6ircruag 349 5 for for IN cord-022235-6ircruag 349 6 fusion fusion NN cord-022235-6ircruag 349 7 between between IN cord-022235-6ircruag 349 8 the the DT cord-022235-6ircruag 349 9 vesicle vesicle NN cord-022235-6ircruag 349 10 and and CC cord-022235-6ircruag 349 11 cytoplasmic cytoplasmic JJ cord-022235-6ircruag 349 12 mem mem NN cord-022235-6ircruag 349 13 brane brane NN cord-022235-6ircruag 349 14 . . . cord-022235-6ircruag 350 1 Saccharomyces saccharomyce NNS cord-022235-6ircruag 350 2 cerevisiae cerevisiae NN cord-022235-6ircruag 350 3 strains strain NNS cord-022235-6ircruag 350 4 carrying carry VBG cord-022235-6ircruag 350 5 a a DT cord-022235-6ircruag 350 6 temperature temperature NN cord-022235-6ircruag 350 7 - - HYPH cord-022235-6ircruag 350 8 sensitive sensitive JJ cord-022235-6ircruag 350 9 mu mu NN cord-022235-6ircruag 350 10 tation tation NN cord-022235-6ircruag 350 11 in in IN cord-022235-6ircruag 350 12 the the DT cord-022235-6ircruag 350 13 SEC4 SEC4 NNP cord-022235-6ircruag 350 14 gene gene NN cord-022235-6ircruag 350 15 accumulate accumulate VBP cord-022235-6ircruag 350 16 large large JJ cord-022235-6ircruag 350 17 numbers number NNS cord-022235-6ircruag 350 18 of of IN cord-022235-6ircruag 350 19 secretory secretory JJ cord-022235-6ircruag 350 20 vesicles vesicle NNS cord-022235-6ircruag 350 21 . . . cord-022235-6ircruag 351 1 These these DT cord-022235-6ircruag 351 2 were be VBD cord-022235-6ircruag 351 3 purified purify VBN cord-022235-6ircruag 351 4 by by IN cord-022235-6ircruag 351 5 Walworth Walworth NNP cord-022235-6ircruag 351 6 and and CC cord-022235-6ircruag 351 7 Novick Novick NNP cord-022235-6ircruag 351 8 ( ( -LRB- cord-022235-6ircruag 351 9 1165 1165 CD cord-022235-6ircruag 351 10 ) ) -RRB- cord-022235-6ircruag 351 11 , , , cord-022235-6ircruag 351 12 who who WP cord-022235-6ircruag 351 13 found find VBD cord-022235-6ircruag 351 14 them -PRON- PRP cord-022235-6ircruag 351 15 to to TO cord-022235-6ircruag 351 16 contain contain VB cord-022235-6ircruag 351 17 three three CD cord-022235-6ircruag 351 18 dominant dominant JJ cord-022235-6ircruag 351 19 proteins protein NNS cord-022235-6ircruag 351 20 ( ( -LRB- cord-022235-6ircruag 351 21 110 110 CD cord-022235-6ircruag 351 22 kDa kDa NNS cord-022235-6ircruag 351 23 , , , cord-022235-6ircruag 351 24 40 40 CD cord-022235-6ircruag 351 25 - - SYM cord-022235-6ircruag 351 26 45 45 CD cord-022235-6ircruag 351 27 kDa kDa NNS cord-022235-6ircruag 351 28 , , , cord-022235-6ircruag 351 29 and and CC cord-022235-6ircruag 351 30 18 18 CD cord-022235-6ircruag 351 31 kDa kDa NNS cord-022235-6ircruag 351 32 ) ) -RRB- cord-022235-6ircruag 351 33 to to TO cord-022235-6ircruag 351 34 gether gether VB cord-022235-6ircruag 351 35 with with IN cord-022235-6ircruag 351 36 invertase invertase NNP cord-022235-6ircruag 351 37 , , , cord-022235-6ircruag 351 38 the the DT cord-022235-6ircruag 351 39 secretory secretory JJ cord-022235-6ircruag 351 40 marker marker NN cord-022235-6ircruag 351 41 protein protein NN cord-022235-6ircruag 351 42 . . . cord-022235-6ircruag 352 1 These these DT cord-022235-6ircruag 352 2 three three CD cord-022235-6ircruag 352 3 proteins protein NNS cord-022235-6ircruag 352 4 were be VBD cord-022235-6ircruag 352 5 made make VBN cord-022235-6ircruag 352 6 during during IN cord-022235-6ircruag 352 7 the the DT cord-022235-6ircruag 352 8 period period NN cord-022235-6ircruag 352 9 in in IN cord-022235-6ircruag 352 10 which which WDT cord-022235-6ircruag 352 11 the the DT cord-022235-6ircruag 352 12 vesicles vesicle NNS cord-022235-6ircruag 352 13 accumulated accumulate VBN cord-022235-6ircruag 352 14 , , , cord-022235-6ircruag 352 15 i.e. i.e. FW cord-022235-6ircruag 352 16 , , , cord-022235-6ircruag 352 17 after after IN cord-022235-6ircruag 352 18 secretion secretion NN cord-022235-6ircruag 352 19 had have VBD cord-022235-6ircruag 352 20 been be VBN cord-022235-6ircruag 352 21 shut shut VBN cord-022235-6ircruag 352 22 down down RP cord-022235-6ircruag 352 23 at at IN cord-022235-6ircruag 352 24 the the DT cord-022235-6ircruag 352 25 nonpermissive nonpermissive JJ cord-022235-6ircruag 352 26 temperature temperature NN cord-022235-6ircruag 352 27 . . . cord-022235-6ircruag 353 1 The the DT cord-022235-6ircruag 353 2 110-kDa 110-kda CD cord-022235-6ircruag 353 3 protein protein NN cord-022235-6ircruag 353 4 was be VBD cord-022235-6ircruag 353 5 the the DT cord-022235-6ircruag 353 6 most most RBS cord-022235-6ircruag 353 7 abundant abundant JJ cord-022235-6ircruag 353 8 protein protein NN cord-022235-6ircruag 353 9 in in IN cord-022235-6ircruag 353 10 the the DT cord-022235-6ircruag 353 11 lumen lumen NN cord-022235-6ircruag 353 12 of of IN cord-022235-6ircruag 353 13 the the DT cord-022235-6ircruag 353 14 secretory secretory JJ cord-022235-6ircruag 353 15 vesicles vesicle NNS cord-022235-6ircruag 353 16 , , , cord-022235-6ircruag 353 17 whereas whereas IN cord-022235-6ircruag 353 18 the the DT cord-022235-6ircruag 353 19 other other JJ cord-022235-6ircruag 353 20 two two CD cord-022235-6ircruag 353 21 proteins protein NNS cord-022235-6ircruag 353 22 were be VBD cord-022235-6ircruag 353 23 membrane membrane NN cord-022235-6ircruag 353 24 - - HYPH cord-022235-6ircruag 353 25 associated associate VBN cord-022235-6ircruag 353 26 and and CC cord-022235-6ircruag 353 27 had have VBD cord-022235-6ircruag 353 28 cytoplasmic cytoplasmic JJ cord-022235-6ircruag 353 29 domains domain NNS cord-022235-6ircruag 353 30 which which WDT cord-022235-6ircruag 353 31 could could MD cord-022235-6ircruag 353 32 interact interact VB cord-022235-6ircruag 353 33 with with IN cord-022235-6ircruag 353 34 the the DT cord-022235-6ircruag 353 35 cytoskeleton cytoskeleton NN cord-022235-6ircruag 353 36 , , , cord-022235-6ircruag 353 37 the the DT cord-022235-6ircruag 353 38 cytoplasmic cytoplasmic JJ cord-022235-6ircruag 353 39 membrane membrane NN cord-022235-6ircruag 353 40 , , , cord-022235-6ircruag 353 41 or or CC cord-022235-6ircruag 353 42 cytoplasmic cytoplasmic JJ cord-022235-6ircruag 353 43 components component NNS cord-022235-6ircruag 353 44 ( ( -LRB- cord-022235-6ircruag 353 45 1165 1165 CD cord-022235-6ircruag 353 46 ) ) -RRB- cord-022235-6ircruag 353 47 . . . cord-022235-6ircruag 354 1 It -PRON- PRP cord-022235-6ircruag 354 2 should should MD cord-022235-6ircruag 354 3 be be VB cord-022235-6ircruag 354 4 noted note VBN cord-022235-6ircruag 354 5 , , , cord-022235-6ircruag 354 6 however however RB cord-022235-6ircruag 354 7 , , , cord-022235-6ircruag 354 8 that that IN cord-022235-6ircruag 354 9 exocytosis exocytosis NN cord-022235-6ircruag 354 10 in in IN cord-022235-6ircruag 354 11 S. S. NNP cord-022235-6ircruag 354 12 cerevisiae cerevisiae NNS cord-022235-6ircruag 354 13 might may MD cord-022235-6ircruag 354 14 be be VB cord-022235-6ircruag 354 15 considered consider VBN cord-022235-6ircruag 354 16 as as IN cord-022235-6ircruag 354 17 polarized polarize VBN cord-022235-6ircruag 354 18 rather rather RB cord-022235-6ircruag 354 19 than than IN cord-022235-6ircruag 354 20 default default NN cord-022235-6ircruag 354 21 sorting sorting NN cord-022235-6ircruag 354 22 because because IN cord-022235-6ircruag 354 23 secretory secretory JJ cord-022235-6ircruag 354 24 vesicles vesicle NNS cord-022235-6ircruag 354 25 are be VBP cord-022235-6ircruag 354 26 di di IN cord-022235-6ircruag 354 27 rected recte VBN cord-022235-6ircruag 354 28 towards towards IN cord-022235-6ircruag 354 29 the the DT cord-022235-6ircruag 354 30 growing grow VBG cord-022235-6ircruag 354 31 bud bud NN cord-022235-6ircruag 354 32 rather rather RB cord-022235-6ircruag 354 33 than than IN cord-022235-6ircruag 354 34 being be VBG cord-022235-6ircruag 354 35 randomly randomly RB cord-022235-6ircruag 354 36 distributed distribute VBN cord-022235-6ircruag 354 37 over over IN cord-022235-6ircruag 354 38 the the DT cord-022235-6ircruag 354 39 entire entire JJ cord-022235-6ircruag 354 40 cell cell NN cord-022235-6ircruag 354 41 surface surface NN cord-022235-6ircruag 354 42 ( ( -LRB- cord-022235-6ircruag 354 43 Section section NN cord-022235-6ircruag 354 44 V.G.2 v.g.2 CD cord-022235-6ircruag 354 45 and and CC cord-022235-6ircruag 354 46 Fig fig NN cord-022235-6ircruag 354 47 . . . cord-022235-6ircruag 354 48 II.5 II.5 NNP cord-022235-6ircruag 354 49 ) ) -RRB- cord-022235-6ircruag 354 50 . . . cord-022235-6ircruag 355 1 The the DT cord-022235-6ircruag 355 2 SEC4 SEC4 NNP cord-022235-6ircruag 355 3 gene gene NN cord-022235-6ircruag 355 4 was be VBD cord-022235-6ircruag 355 5 cloned clone VBN cord-022235-6ircruag 355 6 and and CC cord-022235-6ircruag 355 7 sequenced sequence VBN cord-022235-6ircruag 355 8 by by IN cord-022235-6ircruag 355 9 Salminen Salminen NNP cord-022235-6ircruag 355 10 and and CC cord-022235-6ircruag 355 11 Novick Novick NNP cord-022235-6ircruag 355 12 ( ( -LRB- cord-022235-6ircruag 355 13 987 987 CD cord-022235-6ircruag 355 14 ) ) -RRB- cord-022235-6ircruag 355 15 , , , cord-022235-6ircruag 355 16 who who WP cord-022235-6ircruag 355 17 found find VBD cord-022235-6ircruag 355 18 it -PRON- PRP cord-022235-6ircruag 355 19 to to TO cord-022235-6ircruag 355 20 be be VB cord-022235-6ircruag 355 21 homologous homologous JJ cord-022235-6ircruag 355 22 to to IN cord-022235-6ircruag 355 23 GTP GTP NNP cord-022235-6ircruag 355 24 - - HYPH cord-022235-6ircruag 355 25 binding bind VBG cord-022235-6ircruag 355 26 RAS RAS NNP cord-022235-6ircruag 355 27 regulatory regulatory JJ cord-022235-6ircruag 355 28 proteins protein NNS cord-022235-6ircruag 355 29 of of IN cord-022235-6ircruag 355 30 higher high JJR cord-022235-6ircruag 355 31 eukaryotes eukaryote NNS cord-022235-6ircruag 355 32 . . . cord-022235-6ircruag 356 1 Whether whether IN cord-022235-6ircruag 356 2 this this DT cord-022235-6ircruag 356 3 is be VBZ cord-022235-6ircruag 356 4 significant significant JJ cord-022235-6ircruag 356 5 for for IN cord-022235-6ircruag 356 6 the the DT cord-022235-6ircruag 356 7 role role NN cord-022235-6ircruag 356 8 of of IN cord-022235-6ircruag 356 9 SEC4 SEC4 NNP cord-022235-6ircruag 356 10 in in IN cord-022235-6ircruag 356 11 secretion secretion NN cord-022235-6ircruag 356 12 is be VBZ cord-022235-6ircruag 356 13 unclear unclear JJ cord-022235-6ircruag 356 14 , , , cord-022235-6ircruag 356 15 especially especially RB cord-022235-6ircruag 356 16 since since IN cord-022235-6ircruag 356 17 it -PRON- PRP cord-022235-6ircruag 356 18 is be VBZ cord-022235-6ircruag 356 19 not not RB cord-022235-6ircruag 356 20 known know VBN cord-022235-6ircruag 356 21 whether whether IN cord-022235-6ircruag 356 22 GTP GTP NNP cord-022235-6ircruag 356 23 is be VBZ cord-022235-6ircruag 356 24 required require VBN cord-022235-6ircruag 356 25 for for IN cord-022235-6ircruag 356 26 exocytosis exocytosis NN cord-022235-6ircruag 356 27 in in IN cord-022235-6ircruag 356 28 yeasts yeast NNS cord-022235-6ircruag 356 29 . . . cord-022235-6ircruag 357 1 Recent recent JJ cord-022235-6ircruag 357 2 studies study NNS cord-022235-6ircruag 357 3 show show VBP cord-022235-6ircruag 357 4 that that IN cord-022235-6ircruag 357 5 SEC4 SEC4 NNP cord-022235-6ircruag 357 6 protein protein NN cord-022235-6ircruag 357 7 binds bind VBZ cord-022235-6ircruag 357 8 GTP GTP NNP cord-022235-6ircruag 357 9 ( ( -LRB- cord-022235-6ircruag 357 10 391 391 CD cord-022235-6ircruag 357 11 ) ) -RRB- cord-022235-6ircruag 357 12 . . . cord-022235-6ircruag 358 1 Overexpression overexpression NN cord-022235-6ircruag 358 2 of of IN cord-022235-6ircruag 358 3 SEC4 SEC4 NNP cord-022235-6ircruag 358 4 suppresses suppress VBZ cord-022235-6ircruag 358 5 the the DT cord-022235-6ircruag 358 6 effects effect NNS cord-022235-6ircruag 358 7 of of IN cord-022235-6ircruag 358 8 mutations mutation NNS cord-022235-6ircruag 358 9 in in IN cord-022235-6ircruag 358 10 three three CD cord-022235-6ircruag 358 11 other other JJ cord-022235-6ircruag 358 12 SEC SEC NNP cord-022235-6ircruag 358 13 genes gene NNS cord-022235-6ircruag 358 14 , , , cord-022235-6ircruag 358 15 but but CC cord-022235-6ircruag 358 16 strains strain NNS cord-022235-6ircruag 358 17 carrying carry VBG cord-022235-6ircruag 358 18 sec4ts sec4ts NNP cord-022235-6ircruag 359 1 muta muta NNP cord-022235-6ircruag 359 2 tions tion NNS cord-022235-6ircruag 359 3 rapidly rapidly RB cord-022235-6ircruag 359 4 become become VBP cord-022235-6ircruag 359 5 secretion secretion NN cord-022235-6ircruag 359 6 - - HYPH cord-022235-6ircruag 359 7 defective defective JJ cord-022235-6ircruag 359 8 at at IN cord-022235-6ircruag 359 9 the the DT cord-022235-6ircruag 359 10 nonpermissive nonpermissive JJ cord-022235-6ircruag 359 11 tempera tempera NN cord-022235-6ircruag 359 12 ture ture NN cord-022235-6ircruag 359 13 , , , cord-022235-6ircruag 359 14 suggesting suggest VBG cord-022235-6ircruag 359 15 a a DT cord-022235-6ircruag 359 16 direct direct JJ cord-022235-6ircruag 359 17 role role NN cord-022235-6ircruag 359 18 in in IN cord-022235-6ircruag 359 19 secretion secretion NN cord-022235-6ircruag 359 20 . . . cord-022235-6ircruag 360 1 The the DT cord-022235-6ircruag 360 2 cytoplasmic cytoplasmic JJ cord-022235-6ircruag 360 3 and and CC cord-022235-6ircruag 360 4 secretory secretory JJ cord-022235-6ircruag 360 5 vesicle vesicle NN cord-022235-6ircruag 360 6 membrane membrane NN cord-022235-6ircruag 360 7 - - HYPH cord-022235-6ircruag 360 8 associated associate VBN cord-022235-6ircruag 360 9 SEC4 SEC4 NNP cord-022235-6ircruag 360 10 product product NN cord-022235-6ircruag 360 11 does do VBZ cord-022235-6ircruag 360 12 not not RB cord-022235-6ircruag 360 13 itself -PRON- PRP cord-022235-6ircruag 360 14 appear appear VB cord-022235-6ircruag 360 15 to to TO cord-022235-6ircruag 360 16 be be VB cord-022235-6ircruag 360 17 a a DT cord-022235-6ircruag 360 18 secretory secretory JJ cord-022235-6ircruag 360 19 protein protein NN cord-022235-6ircruag 360 20 because because IN cord-022235-6ircruag 360 21 its -PRON- PRP$ cord-022235-6ircruag 360 22 predicted predict VBN cord-022235-6ircruag 360 23 primary primary JJ cord-022235-6ircruag 360 24 sequence sequence NN cord-022235-6ircruag 360 25 does do VBZ cord-022235-6ircruag 360 26 not not RB cord-022235-6ircruag 360 27 in in IN cord-022235-6ircruag 360 28 clude clude NNP cord-022235-6ircruag 360 29 a a DT cord-022235-6ircruag 360 30 potential potential JJ cord-022235-6ircruag 360 31 secretory secretory NN cord-022235-6ircruag 360 32 routing routing NN cord-022235-6ircruag 360 33 signal signal NN cord-022235-6ircruag 360 34 . . . cord-022235-6ircruag 361 1 One one CD cord-022235-6ircruag 361 2 possibility possibility NN cord-022235-6ircruag 361 3 raised raise VBN cord-022235-6ircruag 361 4 by by IN cord-022235-6ircruag 361 5 Salminen Salminen NNP cord-022235-6ircruag 361 6 and and CC cord-022235-6ircruag 361 7 Novick Novick NNP cord-022235-6ircruag 361 8 is be VBZ cord-022235-6ircruag 361 9 that that IN cord-022235-6ircruag 361 10 the the DT cord-022235-6ircruag 361 11 C C NNP cord-022235-6ircruag 361 12 - - HYPH cord-022235-6ircruag 361 13 terminal terminal NN cord-022235-6ircruag 361 14 cysteine cysteine NN cord-022235-6ircruag 361 15 residue residue NN cord-022235-6ircruag 361 16 of of IN cord-022235-6ircruag 361 17 SEC4 SEC4 NNP cord-022235-6ircruag 361 18 is be VBZ cord-022235-6ircruag 361 19 acylated acylate VBN cord-022235-6ircruag 361 20 , , , cord-022235-6ircruag 361 21 as as IN cord-022235-6ircruag 361 22 are be VBP cord-022235-6ircruag 361 23 the the DT cord-022235-6ircruag 361 24 GTP GTP NNP cord-022235-6ircruag 361 25 - - HYPH cord-022235-6ircruag 361 26 binding bind VBG cord-022235-6ircruag 361 27 RAS RAS NNP cord-022235-6ircruag 361 28 proteins protein NNS cord-022235-6ircruag 361 29 and and CC cord-022235-6ircruag 361 30 that that IN cord-022235-6ircruag 361 31 the the DT cord-022235-6ircruag 361 32 acyl acyl NNP cord-022235-6ircruag 361 33 groups group NNS cord-022235-6ircruag 361 34 anchor anchor VBD cord-022235-6ircruag 361 35 the the DT cord-022235-6ircruag 361 36 polypeptide polypeptide NN cord-022235-6ircruag 361 37 in in IN cord-022235-6ircruag 361 38 the the DT cord-022235-6ircruag 361 39 membrane membrane NN cord-022235-6ircruag 361 40 , , , cord-022235-6ircruag 361 41 but but CC cord-022235-6ircruag 361 42 this this DT cord-022235-6ircruag 361 43 could could MD cord-022235-6ircruag 361 44 not not RB cord-022235-6ircruag 361 45 be be VB cord-022235-6ircruag 361 46 confirmed confirm VBN cord-022235-6ircruag 361 47 directly directly RB cord-022235-6ircruag 361 48 . . . cord-022235-6ircruag 362 1 Another another DT cord-022235-6ircruag 362 2 GTP GTP NNP cord-022235-6ircruag 362 3 binding bind VBG cord-022235-6ircruag 362 4 protein protein NN cord-022235-6ircruag 362 5 , , , cord-022235-6ircruag 362 6 YPT1 YPT1 NNP cord-022235-6ircruag 362 7 ( ( -LRB- cord-022235-6ircruag 362 8 which which WDT cord-022235-6ircruag 362 9 is be VBZ cord-022235-6ircruag 362 10 50 50 CD cord-022235-6ircruag 362 11 % % NN cord-022235-6ircruag 362 12 homologous homologous JJ cord-022235-6ircruag 362 13 to to IN cord-022235-6ircruag 362 14 SEC4 SEC4 NNP cord-022235-6ircruag 362 15 ) ) -RRB- cord-022235-6ircruag 362 16 was be VBD cord-022235-6ircruag 362 17 detected detect VBN cord-022235-6ircruag 362 18 close close RB cord-022235-6ircruag 362 19 to to IN cord-022235-6ircruag 362 20 the the DT cord-022235-6ircruag 362 21 bud bud NN cord-022235-6ircruag 362 22 as as RB cord-022235-6ircruag 362 23 well well RB cord-022235-6ircruag 362 24 as as IN cord-022235-6ircruag 362 25 in in IN cord-022235-6ircruag 362 26 ill ill RB cord-022235-6ircruag 362 27 - - HYPH cord-022235-6ircruag 362 28 defined define VBN cord-022235-6ircruag 362 29 structures structure NNS cord-022235-6ircruag 362 30 ( ( -LRB- cord-022235-6ircruag 362 31 possibly possibly RB cord-022235-6ircruag 362 32 the the DT cord-022235-6ircruag 362 33 ER ER NNP cord-022235-6ircruag 362 34 and and CC cord-022235-6ircruag 362 35 Golgi Golgi NNP cord-022235-6ircruag 362 36 ) ) -RRB- cord-022235-6ircruag 362 37 of of IN cord-022235-6ircruag 362 38 S. S. NNP cord-022235-6ircruag 362 39 cerevisiae cerevisiae NN cord-022235-6ircruag 362 40 cells cell NNS cord-022235-6ircruag 362 41 ( ( -LRB- cord-022235-6ircruag 362 42 1030 1030 CD cord-022235-6ircruag 362 43 ) ) -RRB- cord-022235-6ircruag 362 44 . . . cord-022235-6ircruag 363 1 Mutations mutation NNS cord-022235-6ircruag 363 2 in in IN cord-022235-6ircruag 363 3 the the DT cord-022235-6ircruag 363 4 YPT1 YPT1 NNP cord-022235-6ircruag 363 5 gene gene NN cord-022235-6ircruag 363 6 affect affect VBP cord-022235-6ircruag 363 7 several several JJ cord-022235-6ircruag 363 8 stages stage NNS cord-022235-6ircruag 363 9 in in IN cord-022235-6ircruag 363 10 the the DT cord-022235-6ircruag 363 11 secretory secretory JJ cord-022235-6ircruag 363 12 pathway pathway NN cord-022235-6ircruag 363 13 , , , cord-022235-6ircruag 363 14 but but CC cord-022235-6ircruag 363 15 YPT1 YPT1 NNP cord-022235-6ircruag 363 16 pro pro NNP cord-022235-6ircruag 363 17 tein tein NNP cord-022235-6ircruag 363 18 probably probably RB cord-022235-6ircruag 363 19 plays play VBZ cord-022235-6ircruag 363 20 an an DT cord-022235-6ircruag 363 21 indirect indirect JJ cord-022235-6ircruag 363 22 role role NN cord-022235-6ircruag 363 23 in in IN cord-022235-6ircruag 363 24 protein protein NN cord-022235-6ircruag 363 25 transport transport NN cord-022235-6ircruag 363 26 as as IN cord-022235-6ircruag 363 27 a a DT cord-022235-6ircruag 363 28 result result NN cord-022235-6ircruag 363 29 of of IN cord-022235-6ircruag 363 30 its -PRON- PRP$ cord-022235-6ircruag 363 31 role role NN cord-022235-6ircruag 363 32 in in IN cord-022235-6ircruag 363 33 Ca Ca NNP cord-022235-6ircruag 363 34 2 2 CD cord-022235-6ircruag 363 35 + + SYM cord-022235-6ircruag 363 36 regulation regulation NN cord-022235-6ircruag 363 37 ( ( -LRB- cord-022235-6ircruag 363 38 1014 1014 CD cord-022235-6ircruag 363 39 ) ) -RRB- cord-022235-6ircruag 363 40 . . . cord-022235-6ircruag 364 1 The the DT cord-022235-6ircruag 364 2 plasma plasma NN cord-022235-6ircruag 364 3 membrane membrane NN cord-022235-6ircruag 364 4 of of IN cord-022235-6ircruag 364 5 epithelial epithelial JJ cord-022235-6ircruag 364 6 cells cell NNS cord-022235-6ircruag 364 7 is be VBZ cord-022235-6ircruag 364 8 divided divide VBN cord-022235-6ircruag 364 9 into into IN cord-022235-6ircruag 364 10 two two CD cord-022235-6ircruag 364 11 distinct distinct JJ cord-022235-6ircruag 364 12 do do VBP cord-022235-6ircruag 364 13 mains main NNS cord-022235-6ircruag 364 14 . . . cord-022235-6ircruag 365 1 The the DT cord-022235-6ircruag 365 2 apical apical JJ cord-022235-6ircruag 365 3 surface surface NN cord-022235-6ircruag 365 4 , , , cord-022235-6ircruag 365 5 which which WDT cord-022235-6ircruag 365 6 may may MD cord-022235-6ircruag 365 7 have have VB cord-022235-6ircruag 365 8 microvilli microvilli NN cord-022235-6ircruag 365 9 , , , cord-022235-6ircruag 365 10 is be VBZ cord-022235-6ircruag 365 11 oriented orient VBN cord-022235-6ircruag 365 12 toward toward IN cord-022235-6ircruag 365 13 the the DT cord-022235-6ircruag 365 14 outside outside NN cord-022235-6ircruag 365 15 ( ( -LRB- cord-022235-6ircruag 365 16 e.g. e.g. RB cord-022235-6ircruag 365 17 , , , cord-022235-6ircruag 365 18 lumen lumen NN cord-022235-6ircruag 365 19 of of IN cord-022235-6ircruag 365 20 the the DT cord-022235-6ircruag 365 21 intestine intestine NN cord-022235-6ircruag 365 22 ) ) -RRB- cord-022235-6ircruag 365 23 , , , cord-022235-6ircruag 365 24 whereas whereas IN cord-022235-6ircruag 365 25 the the DT cord-022235-6ircruag 365 26 basolateral basolateral JJ cord-022235-6ircruag 365 27 surface surface NN cord-022235-6ircruag 365 28 is be VBZ cord-022235-6ircruag 365 29 on on IN cord-022235-6ircruag 365 30 the the DT cord-022235-6ircruag 365 31 inside inside NN cord-022235-6ircruag 365 32 , , , cord-022235-6ircruag 365 33 facing face VBG cord-022235-6ircruag 365 34 the the DT cord-022235-6ircruag 365 35 basolateral basolateral JJ cord-022235-6ircruag 365 36 surface surface NN cord-022235-6ircruag 365 37 of of IN cord-022235-6ircruag 365 38 other other JJ cord-022235-6ircruag 365 39 cells cell NNS cord-022235-6ircruag 365 40 or or CC cord-022235-6ircruag 365 41 resting rest VBG cord-022235-6ircruag 365 42 on on IN cord-022235-6ircruag 365 43 an an DT cord-022235-6ircruag 365 44 extracellular extracellular JJ cord-022235-6ircruag 365 45 matrix matrix NN cord-022235-6ircruag 365 46 of of IN cord-022235-6ircruag 365 47 basal basal NN cord-022235-6ircruag 365 48 lamina lamina NNP cord-022235-6ircruag 365 49 ( ( -LRB- cord-022235-6ircruag 365 50 Fig Fig NNP cord-022235-6ircruag 365 51 . . . cord-022235-6ircruag 365 52 V.5 v.5 NN cord-022235-6ircruag 365 53 ) ) -RRB- cord-022235-6ircruag 365 54 . . . cord-022235-6ircruag 366 1 The the DT cord-022235-6ircruag 366 2 two two CD cord-022235-6ircruag 366 3 membrane membrane NN cord-022235-6ircruag 366 4 domains domain NNS cord-022235-6ircruag 366 5 have have VBP cord-022235-6ircruag 366 6 distinctly distinctly RB cord-022235-6ircruag 366 7 different different JJ cord-022235-6ircruag 366 8 lipid lipid NN cord-022235-6ircruag 366 9 contents content NNS cord-022235-6ircruag 366 10 in in IN cord-022235-6ircruag 366 11 their -PRON- PRP$ cord-022235-6ircruag 366 12 outer outer JJ cord-022235-6ircruag 366 13 leaflets leaflet NNS cord-022235-6ircruag 366 14 [ [ -LRB- cord-022235-6ircruag 366 15 the the DT cord-022235-6ircruag 366 16 apical apical JJ cord-022235-6ircruag 366 17 membrane membrane NN cord-022235-6ircruag 366 18 has have VBZ cord-022235-6ircruag 366 19 a a DT cord-022235-6ircruag 366 20 higher high JJR cord-022235-6ircruag 366 21 glycolipid glycolipid NN cord-022235-6ircruag 366 22 and and CC cord-022235-6ircruag 366 23 cholesterol cholesterol NN cord-022235-6ircruag 366 24 content content NN cord-022235-6ircruag 366 25 , , , cord-022235-6ircruag 366 26 and and CC cord-022235-6ircruag 366 27 a a DT cord-022235-6ircruag 366 28 lower low JJR cord-022235-6ircruag 366 29 phosphatidylcholine phosphatidylcholine NN cord-022235-6ircruag 366 30 content content NN cord-022235-6ircruag 366 31 than than IN cord-022235-6ircruag 366 32 the the DT cord-022235-6ircruag 366 33 basolateral basolateral JJ cord-022235-6ircruag 366 34 membrane membrane NN cord-022235-6ircruag 366 35 ( ( -LRB- cord-022235-6ircruag 366 36 1050 1050 CD cord-022235-6ircruag 366 37 ) ) -RRB- cord-022235-6ircruag 366 38 ] ] -RRB- cord-022235-6ircruag 366 39 , , , cord-022235-6ircruag 366 40 although although IN cord-022235-6ircruag 366 41 the the DT cord-022235-6ircruag 366 42 lipid lipid NN cord-022235-6ircruag 366 43 contents content NNS cord-022235-6ircruag 366 44 of of IN cord-022235-6ircruag 366 45 their -PRON- PRP$ cord-022235-6ircruag 366 46 inner inner JJ cord-022235-6ircruag 366 47 leaflets leaflet NNS cord-022235-6ircruag 366 48 may may MD cord-022235-6ircruag 366 49 be be VB cord-022235-6ircruag 366 50 identical identical JJ cord-022235-6ircruag 366 51 ( ( -LRB- cord-022235-6ircruag 366 52 inset inset NN cord-022235-6ircruag 366 53 to to IN cord-022235-6ircruag 366 54 Fig Fig NNP cord-022235-6ircruag 366 55 . . . cord-022235-6ircruag 366 56 V.5 v.5 NN cord-022235-6ircruag 366 57 ) ) -RRB- cord-022235-6ircruag 366 58 . . . cord-022235-6ircruag 367 1 This this DT cord-022235-6ircruag 367 2 implies imply VBZ cord-022235-6ircruag 367 3 that that IN cord-022235-6ircruag 367 4 only only RB cord-022235-6ircruag 367 5 the the DT cord-022235-6ircruag 367 6 outer outer JJ cord-022235-6ircruag 367 7 leaflets leaflet NNS cord-022235-6ircruag 367 8 of of IN cord-022235-6ircruag 367 9 the the DT cord-022235-6ircruag 367 10 two two CD cord-022235-6ircruag 367 11 membranes membrane NNS cord-022235-6ircruag 367 12 are be VBP cord-022235-6ircruag 367 13 separated separate VBN cord-022235-6ircruag 367 14 by by IN cord-022235-6ircruag 367 15 tight tight JJ cord-022235-6ircruag 367 16 junctions junction NNS cord-022235-6ircruag 367 17 , , , cord-022235-6ircruag 367 18 morphologically morphologically RB cord-022235-6ircruag 367 19 distinct distinct JJ cord-022235-6ircruag 367 20 structures structure NNS cord-022235-6ircruag 367 21 rich rich JJ cord-022235-6ircruag 367 22 in in IN cord-022235-6ircruag 367 23 nonbilayer nonbilayer JJ cord-022235-6ircruag 367 24 lipids lipid NNS cord-022235-6ircruag 367 25 and and CC cord-022235-6ircruag 367 26 containing contain VBG cord-022235-6ircruag 367 27 unique unique JJ cord-022235-6ircruag 367 28 proteins protein NNS cord-022235-6ircruag 367 29 which which WDT cord-022235-6ircruag 367 30 form form VBP cord-022235-6ircruag 367 31 the the DT cord-022235-6ircruag 367 32 junction junction NN cord-022235-6ircruag 367 33 between between IN cord-022235-6ircruag 367 34 apical apical JJ cord-022235-6ircruag 367 35 and and CC cord-022235-6ircruag 367 36 basolateral basolateral JJ cord-022235-6ircruag 367 37 surfaces surface NNS cord-022235-6ircruag 367 38 and and CC cord-022235-6ircruag 367 39 probably probably RB cord-022235-6ircruag 367 40 prevent prevent VB cord-022235-6ircruag 367 41 the the DT cord-022235-6ircruag 367 42 movement movement NN cord-022235-6ircruag 367 43 of of IN cord-022235-6ircruag 367 44 outer outer JJ cord-022235-6ircruag 367 45 leaflet leaflet NN cord-022235-6ircruag 367 46 lipids lipid NNS cord-022235-6ircruag 367 47 and and CC cord-022235-6ircruag 367 48 proteins protein NNS cord-022235-6ircruag 367 49 between between IN cord-022235-6ircruag 367 50 them -PRON- PRP cord-022235-6ircruag 367 51 , , , cord-022235-6ircruag 367 52 as as RB cord-022235-6ircruag 367 53 well well RB cord-022235-6ircruag 367 54 as as IN cord-022235-6ircruag 367 55 acting act VBG cord-022235-6ircruag 367 56 as as IN cord-022235-6ircruag 367 57 ion ion NN cord-022235-6ircruag 367 58 gates gate NNS cord-022235-6ircruag 367 59 between between IN cord-022235-6ircruag 367 60 adjacent adjacent JJ cord-022235-6ircruag 367 61 cells cell NNS cord-022235-6ircruag 367 62 ( ( -LRB- cord-022235-6ircruag 367 63 Fig fig NN cord-022235-6ircruag 367 64 . . . cord-022235-6ircruag 368 1 V V NNP cord-022235-6ircruag 368 2 .5 .5 CD cord-022235-6ircruag 368 3 ) ) -RRB- cord-022235-6ircruag 368 4 ( ( -LRB- cord-022235-6ircruag 368 5 177 177 CD cord-022235-6ircruag 368 6 , , , cord-022235-6ircruag 368 7 260 260 CD cord-022235-6ircruag 368 8 , , , cord-022235-6ircruag 368 9 409 409 CD cord-022235-6ircruag 368 10 , , , cord-022235-6ircruag 368 11 ( ( -LRB- cord-022235-6ircruag 368 12 706 706 CD cord-022235-6ircruag 368 13 ) ) -RRB- cord-022235-6ircruag 368 14 ( ( -LRB- cord-022235-6ircruag 368 15 707 707 CD cord-022235-6ircruag 368 16 ) ) -RRB- cord-022235-6ircruag 368 17 ( ( -LRB- cord-022235-6ircruag 368 18 708 708 CD cord-022235-6ircruag 368 19 ) ) -RRB- cord-022235-6ircruag 368 20 1050 1050 CD cord-022235-6ircruag 368 21 ) ) -RRB- cord-022235-6ircruag 368 22 . . . cord-022235-6ircruag 369 1 There there EX cord-022235-6ircruag 369 2 may may MD cord-022235-6ircruag 369 3 also also RB cord-022235-6ircruag 369 4 be be VB cord-022235-6ircruag 369 5 differences difference NNS cord-022235-6ircruag 369 6 in in IN cord-022235-6ircruag 369 7 basal basal NN cord-022235-6ircruag 369 8 and and CC cord-022235-6ircruag 369 9 lateral lateral JJ cord-022235-6ircruag 369 10 membrane membrane NN cord-022235-6ircruag 369 11 composition composition NN cord-022235-6ircruag 369 12 . . . cord-022235-6ircruag 370 1 Cell cell NN cord-022235-6ircruag 370 2 - - HYPH cord-022235-6ircruag 370 3 cell cell NN cord-022235-6ircruag 370 4 interactions interaction NNS cord-022235-6ircruag 370 5 are be VBP cord-022235-6ircruag 370 6 required require VBN cord-022235-6ircruag 370 7 for for IN cord-022235-6ircruag 370 8 effi effi NNP cord-022235-6ircruag 370 9 cient cient NNP cord-022235-6ircruag 370 10 formation formation NN cord-022235-6ircruag 370 11 of of IN cord-022235-6ircruag 370 12 the the DT cord-022235-6ircruag 370 13 basolateral basolateral JJ cord-022235-6ircruag 370 14 surface surface NN cord-022235-6ircruag 370 15 of of IN cord-022235-6ircruag 370 16 polarized polarized JJ cord-022235-6ircruag 370 17 cells cell NNS cord-022235-6ircruag 370 18 , , , cord-022235-6ircruag 370 19 but but CC cord-022235-6ircruag 370 20 not not RB cord-022235-6ircruag 370 21 for for IN cord-022235-6ircruag 370 22 sorting sort VBG cord-022235-6ircruag 370 23 to to IN cord-022235-6ircruag 370 24 the the DT cord-022235-6ircruag 370 25 apical apical JJ cord-022235-6ircruag 370 26 zone zone NN cord-022235-6ircruag 370 27 ( ( -LRB- cord-022235-6ircruag 370 28 1143 1143 CD cord-022235-6ircruag 370 29 ) ) -RRB- cord-022235-6ircruag 370 30 . . . cord-022235-6ircruag 371 1 Adjacent adjacent JJ cord-022235-6ircruag 371 2 cells cell NNS cord-022235-6ircruag 371 3 may may MD cord-022235-6ircruag 371 4 be be VB cord-022235-6ircruag 371 5 held hold VBN cord-022235-6ircruag 371 6 together together RB cord-022235-6ircruag 371 7 by by IN cord-022235-6ircruag 371 8 desmosomes desmosome NNS cord-022235-6ircruag 371 9 . . . cord-022235-6ircruag 372 1 The the DT cord-022235-6ircruag 372 2 major major JJ cord-022235-6ircruag 372 3 breakthrough breakthrough NN cord-022235-6ircruag 372 4 in in IN cord-022235-6ircruag 372 5 studies study NNS cord-022235-6ircruag 372 6 on on IN cord-022235-6ircruag 372 7 protein protein NN cord-022235-6ircruag 372 8 sorting sorting NN cord-022235-6ircruag 372 9 in in IN cord-022235-6ircruag 372 10 polarized polarized JJ cord-022235-6ircruag 372 11 cells cell NNS cord-022235-6ircruag 372 12 ( ( -LRB- cord-022235-6ircruag 372 13 878 878 CD cord-022235-6ircruag 372 14 ) ) -RRB- cord-022235-6ircruag 372 15 . . . cord-022235-6ircruag 373 1 In in IN cord-022235-6ircruag 373 2 general general JJ cord-022235-6ircruag 373 3 , , , cord-022235-6ircruag 373 4 the the DT cord-022235-6ircruag 373 5 steady steady JJ cord-022235-6ircruag 373 6 - - HYPH cord-022235-6ircruag 373 7 state state NN cord-022235-6ircruag 373 8 distribution distribution NN cord-022235-6ircruag 373 9 of of IN cord-022235-6ircruag 373 10 polarized polarized JJ cord-022235-6ircruag 373 11 membrane membrane NN cord-022235-6ircruag 373 12 proteins protein NNS cord-022235-6ircruag 373 13 indicates indicate VBZ cord-022235-6ircruag 373 14 that that IN cord-022235-6ircruag 373 15 sorting sorting NN cord-022235-6ircruag 373 16 of of IN cord-022235-6ircruag 373 17 basolateral basolateral JJ cord-022235-6ircruag 373 18 pro pro FW cord-022235-6ircruag 373 19 tein tein NN cord-022235-6ircruag 373 20 is be VBZ cord-022235-6ircruag 373 21 highly highly RB cord-022235-6ircruag 373 22 efficient efficient JJ cord-022235-6ircruag 373 23 ( ( -LRB- cord-022235-6ircruag 373 24 > > NFP cord-022235-6ircruag 373 25 97 97 CD cord-022235-6ircruag 373 26 % % NN cord-022235-6ircruag 373 27 fidelity fidelity NN cord-022235-6ircruag 373 28 ) ) -RRB- cord-022235-6ircruag 373 29 ( ( -LRB- cord-022235-6ircruag 373 30 873 873 CD cord-022235-6ircruag 373 31 ) ) -RRB- cord-022235-6ircruag 373 32 , , , cord-022235-6ircruag 373 33 whereas whereas IN cord-022235-6ircruag 373 34 apical apical JJ cord-022235-6ircruag 373 35 proteins protein NNS cord-022235-6ircruag 373 36 may may MD cord-022235-6ircruag 373 37 be be VB cord-022235-6ircruag 373 38 found find VBN cord-022235-6ircruag 373 39 in in IN cord-022235-6ircruag 373 40 significant significant JJ cord-022235-6ircruag 373 41 amounts amount NNS cord-022235-6ircruag 373 42 in in IN cord-022235-6ircruag 373 43 the the DT cord-022235-6ircruag 373 44 basolateral basolateral JJ cord-022235-6ircruag 373 45 membrane membrane NN cord-022235-6ircruag 373 46 . . . cord-022235-6ircruag 374 1 However however RB cord-022235-6ircruag 374 2 , , , cord-022235-6ircruag 374 3 the the DT cord-022235-6ircruag 374 4 surface surface NN cord-022235-6ircruag 374 5 area area NN cord-022235-6ircruag 374 6 of of IN cord-022235-6ircruag 374 7 the the DT cord-022235-6ircruag 374 8 apical apical JJ cord-022235-6ircruag 374 9 membrane membrane NN cord-022235-6ircruag 374 10 is be VBZ cord-022235-6ircruag 374 11 usually usually RB cord-022235-6ircruag 374 12 much much RB cord-022235-6ircruag 374 13 smaller small JJR cord-022235-6ircruag 374 14 than than IN cord-022235-6ircruag 374 15 that that DT cord-022235-6ircruag 374 16 of of IN cord-022235-6ircruag 374 17 the the DT cord-022235-6ircruag 374 18 basolateral basolateral JJ cord-022235-6ircruag 374 19 membrane membrane NN cord-022235-6ircruag 374 20 , , , cord-022235-6ircruag 374 21 which which WDT cord-022235-6ircruag 374 22 means mean VBZ cord-022235-6ircruag 374 23 that that IN cord-022235-6ircruag 374 24 the the DT cord-022235-6ircruag 374 25 fidelity fidelity NN cord-022235-6ircruag 374 26 of of IN cord-022235-6ircruag 374 27 apical apical JJ cord-022235-6ircruag 374 28 tar tar NN cord-022235-6ircruag 374 29 geting geting NN cord-022235-6ircruag 374 30 is be VBZ cord-022235-6ircruag 374 31 actually actually RB cord-022235-6ircruag 374 32 quite quite RB cord-022235-6ircruag 374 33 high high JJ cord-022235-6ircruag 374 34 ( ( -LRB- cord-022235-6ircruag 374 35 about about RB cord-022235-6ircruag 374 36 88 88 CD cord-022235-6ircruag 374 37 % % NN cord-022235-6ircruag 374 38 ) ) -RRB- cord-022235-6ircruag 374 39 ( ( -LRB- cord-022235-6ircruag 374 40 873 873 CD cord-022235-6ircruag 374 41 ) ) -RRB- cord-022235-6ircruag 374 42 . . . cord-022235-6ircruag 375 1 Basolateral Basolateral NNP cord-022235-6ircruag 375 2 and and CC cord-022235-6ircruag 375 3 apical apical JJ cord-022235-6ircruag 375 4 sorting sorting NN cord-022235-6ircruag 375 5 seem seem VBP cord-022235-6ircruag 375 6 to to TO cord-022235-6ircruag 375 7 represent represent VB cord-022235-6ircruag 375 8 two two CD cord-022235-6ircruag 375 9 distinct distinct JJ cord-022235-6ircruag 375 10 pathways pathway NNS cord-022235-6ircruag 375 11 , , , cord-022235-6ircruag 375 12 but but CC cord-022235-6ircruag 375 13 most most JJS cord-022235-6ircruag 375 14 polarized polarized JJ cord-022235-6ircruag 375 15 cells cell NNS cord-022235-6ircruag 375 16 also also RB cord-022235-6ircruag 375 17 secrete secrete VBP cord-022235-6ircruag 375 18 some some DT cord-022235-6ircruag 375 19 proteins protein NNS cord-022235-6ircruag 375 20 from from IN cord-022235-6ircruag 375 21 both both DT cord-022235-6ircruag 375 22 surfaces surface NNS cord-022235-6ircruag 375 23 , , , cord-022235-6ircruag 375 24 implying imply VBG cord-022235-6ircruag 375 25 that that IN cord-022235-6ircruag 375 26 neither neither CC cord-022235-6ircruag 375 27 basolateral basolateral JJ cord-022235-6ircruag 375 28 nor nor CC cord-022235-6ircruag 375 29 apical apical JJ cord-022235-6ircruag 375 30 sorting sorting NN cord-022235-6ircruag 375 31 are be VBP cord-022235-6ircruag 375 32 default default NN cord-022235-6ircruag 375 33 pathways pathway NNS cord-022235-6ircruag 375 34 ( ( -LRB- cord-022235-6ircruag 375 35 390,574 390,574 CD cord-022235-6ircruag 375 36 ) ) -RRB- cord-022235-6ircruag 375 37 . . . cord-022235-6ircruag 376 1 Furthermore furthermore RB cord-022235-6ircruag 376 2 , , , cord-022235-6ircruag 376 3 lysosomal lysosomal JJ cord-022235-6ircruag 376 4 proteins protein NNS cord-022235-6ircruag 376 5 are be VBP cord-022235-6ircruag 376 6 secreted secrete VBN cord-022235-6ircruag 376 7 by by IN cord-022235-6ircruag 376 8 the the DT cord-022235-6ircruag 376 9 default default NN cord-022235-6ircruag 376 10 pathway pathway NN cord-022235-6ircruag 376 11 from from IN cord-022235-6ircruag 376 12 both both CC cord-022235-6ircruag 376 13 apical apical JJ cord-022235-6ircruag 376 14 and and CC cord-022235-6ircruag 376 15 basolateral basolateral JJ cord-022235-6ircruag 376 16 surfaces surface NNS cord-022235-6ircruag 376 17 when when WRB cord-022235-6ircruag 376 18 lysosomal lysosomal JJ cord-022235-6ircruag 376 19 sort sort NN cord-022235-6ircruag 376 20 ing ing NN cord-022235-6ircruag 376 21 is be VBZ cord-022235-6ircruag 376 22 blocked block VBN cord-022235-6ircruag 376 23 ( ( -LRB- cord-022235-6ircruag 376 24 140 140 CD cord-022235-6ircruag 376 25 ) ) -RRB- cord-022235-6ircruag 376 26 . . . cord-022235-6ircruag 377 1 This this DT cord-022235-6ircruag 377 2 may may MD cord-022235-6ircruag 377 3 not not RB cord-022235-6ircruag 377 4 be be VB cord-022235-6ircruag 377 5 the the DT cord-022235-6ircruag 377 6 case case NN cord-022235-6ircruag 377 7 in in IN cord-022235-6ircruag 377 8 Caco-2 Caco-2 NNP cord-022235-6ircruag 377 9 cells cell NNS cord-022235-6ircruag 377 10 in in IN cord-022235-6ircruag 377 11 which which WDT cord-022235-6ircruag 377 12 high high JJ cord-022235-6ircruag 377 13 - - HYPH cord-022235-6ircruag 377 14 level level NN cord-022235-6ircruag 377 15 basolateral basolateral JJ cord-022235-6ircruag 377 16 secretion secretion NN cord-022235-6ircruag 377 17 of of IN cord-022235-6ircruag 377 18 normally normally RB cord-022235-6ircruag 377 19 nonpolarized nonpolarize VBN cord-022235-6ircruag 377 20 lipoproteins lipoprotein NNS cord-022235-6ircruag 377 21 i -PRON- PRP cord-022235-6ircruag 377 22 m be VBP cord-022235-6ircruag 377 23 plies ply VBZ cord-022235-6ircruag 377 24 that that IN cord-022235-6ircruag 377 25 the the DT cord-022235-6ircruag 377 26 basolateral basolateral JJ cord-022235-6ircruag 377 27 sorting sorting NN cord-022235-6ircruag 377 28 predominates predominate VBZ cord-022235-6ircruag 377 29 over over IN cord-022235-6ircruag 377 30 all all DT cord-022235-6ircruag 377 31 other other JJ cord-022235-6ircruag 377 32 sorting sort VBG cord-022235-6ircruag 377 33 path path NN cord-022235-6ircruag 377 34 ways way NNS cord-022235-6ircruag 377 35 , , , cord-022235-6ircruag 377 36 including include VBG cord-022235-6ircruag 377 37 the the DT cord-022235-6ircruag 377 38 default default NN cord-022235-6ircruag 377 39 pathway pathway NN cord-022235-6ircruag 377 40 ( ( -LRB- cord-022235-6ircruag 377 41 136,930a 136,930a CD cord-022235-6ircruag 377 42 ) ) -RRB- cord-022235-6ircruag 377 43 . . . cord-022235-6ircruag 378 1 Do do VBP cord-022235-6ircruag 378 2 polarized polarized JJ cord-022235-6ircruag 378 3 cells cell NNS cord-022235-6ircruag 378 4 sort sort RB cord-022235-6ircruag 378 5 and and CC cord-022235-6ircruag 378 6 direct direct JJ cord-022235-6ircruag 378 7 proteins protein NNS cord-022235-6ircruag 378 8 directly directly RB cord-022235-6ircruag 378 9 to to IN cord-022235-6ircruag 378 10 their -PRON- PRP$ cord-022235-6ircruag 378 11 target target NN cord-022235-6ircruag 378 12 mem mem NNS cord-022235-6ircruag 378 13 branes brane NNS cord-022235-6ircruag 378 14 , , , cord-022235-6ircruag 378 15 or or CC cord-022235-6ircruag 378 16 are be VBP cord-022235-6ircruag 378 17 they -PRON- PRP cord-022235-6ircruag 378 18 all all DT cord-022235-6ircruag 378 19 first first RB cord-022235-6ircruag 378 20 randomly randomly RB cord-022235-6ircruag 378 21 targeted target VBN cord-022235-6ircruag 378 22 to to IN cord-022235-6ircruag 378 23 both both DT cord-022235-6ircruag 378 24 domains domain NNS cord-022235-6ircruag 378 25 , , , cord-022235-6ircruag 378 26 or or CC cord-022235-6ircruag 378 27 specifi specifi VBN cord-022235-6ircruag 378 28 cally cally RB cord-022235-6ircruag 378 29 to to IN cord-022235-6ircruag 378 30 one one CD cord-022235-6ircruag 378 31 or or CC cord-022235-6ircruag 378 32 other other JJ cord-022235-6ircruag 378 33 domain domain NN cord-022235-6ircruag 378 34 , , , cord-022235-6ircruag 378 35 and and CC cord-022235-6ircruag 378 36 then then RB cord-022235-6ircruag 378 37 transcytosed transcytose VBN cord-022235-6ircruag 378 38 ? ? . cord-022235-6ircruag 379 1 Although although IN cord-022235-6ircruag 379 2 some some DT cord-022235-6ircruag 379 3 studies study NNS cord-022235-6ircruag 379 4 designed design VBN cord-022235-6ircruag 379 5 to to TO cord-022235-6ircruag 379 6 answer answer VB cord-022235-6ircruag 379 7 this this DT cord-022235-6ircruag 379 8 question question NN cord-022235-6ircruag 379 9 have have VBP cord-022235-6ircruag 379 10 been be VBN cord-022235-6ircruag 379 11 conducted conduct VBN cord-022235-6ircruag 379 12 with with IN cord-022235-6ircruag 379 13 en en FW cord-022235-6ircruag 379 14 dogenous dogenous JJ cord-022235-6ircruag 379 15 proteins protein NNS cord-022235-6ircruag 379 16 , , , cord-022235-6ircruag 379 17 the the DT cord-022235-6ircruag 379 18 most most RBS cord-022235-6ircruag 379 19 detailed detailed JJ cord-022235-6ircruag 379 20 results result NNS cord-022235-6ircruag 379 21 come come VBP cord-022235-6ircruag 379 22 from from IN cord-022235-6ircruag 379 23 studies study NNS cord-022235-6ircruag 379 24 on on IN cord-022235-6ircruag 379 25 the the DT cord-022235-6ircruag 379 26 basolaterally basolaterally RB cord-022235-6ircruag 379 27 targeted target VBN cord-022235-6ircruag 379 28 VSV VSV NNP cord-022235-6ircruag 379 29 G G NNP cord-022235-6ircruag 379 30 protein protein NN cord-022235-6ircruag 379 31 and and CC cord-022235-6ircruag 379 32 the the DT cord-022235-6ircruag 379 33 apically apically RB cord-022235-6ircruag 379 34 targeted target VBN cord-022235-6ircruag 379 35 influenza influenza NN cord-022235-6ircruag 379 36 virus virus NN cord-022235-6ircruag 379 37 hemagglutinin hemagglutinin NN cord-022235-6ircruag 379 38 ( ( -LRB- cord-022235-6ircruag 379 39 HA HA NNP cord-022235-6ircruag 379 40 ) ) -RRB- cord-022235-6ircruag 379 41 in in IN cord-022235-6ircruag 379 42 MDCK MDCK NNP cord-022235-6ircruag 379 43 cells cell NNS cord-022235-6ircruag 379 44 . . . cord-022235-6ircruag 380 1 Furthermore furthermore RB cord-022235-6ircruag 380 2 , , , cord-022235-6ircruag 380 3 it -PRON- PRP cord-022235-6ircruag 380 4 is be VBZ cord-022235-6ircruag 380 5 through through IN cord-022235-6ircruag 380 6 derivatives derivative NNS cord-022235-6ircruag 380 7 of of IN cord-022235-6ircruag 380 8 these these DT cord-022235-6ircruag 380 9 proteins protein NNS cord-022235-6ircruag 380 10 that that WDT cord-022235-6ircruag 380 11 most most JJS cord-022235-6ircruag 380 12 recent recent JJ cord-022235-6ircruag 380 13 studies study NNS cord-022235-6ircruag 380 14 have have VBP cord-022235-6ircruag 380 15 attempted attempt VBN cord-022235-6ircruag 380 16 to to TO cord-022235-6ircruag 380 17 identify identify VB cord-022235-6ircruag 380 18 apical apical JJ cord-022235-6ircruag 380 19 and and CC cord-022235-6ircruag 380 20 basolateral basolateral JJ cord-022235-6ircruag 380 21 targeting targeting NN cord-022235-6ircruag 380 22 signals signal NNS cord-022235-6ircruag 380 23 ( ( -LRB- cord-022235-6ircruag 380 24 see see VB cord-022235-6ircruag 380 25 next next JJ cord-022235-6ircruag 380 26 section section NN cord-022235-6ircruag 380 27 ) ) -RRB- cord-022235-6ircruag 380 28 . . . cord-022235-6ircruag 381 1 The the DT cord-022235-6ircruag 381 2 results result NNS cord-022235-6ircruag 381 3 of of IN cord-022235-6ircruag 381 4 pulse pulse NN cord-022235-6ircruag 381 5 - - HYPH cord-022235-6ircruag 381 6 chase chase NN cord-022235-6ircruag 381 7 experiments experiment NNS cord-022235-6ircruag 381 8 combined combine VBN cord-022235-6ircruag 381 9 with with IN cord-022235-6ircruag 381 10 a a DT cord-022235-6ircruag 381 11 trypsin trypsin JJ cord-022235-6ircruag 381 12 sensi sensi NN cord-022235-6ircruag 381 13 tivity tivity NN cord-022235-6ircruag 381 14 assay assay NN cord-022235-6ircruag 381 15 and and CC cord-022235-6ircruag 381 16 tests test NNS cord-022235-6ircruag 381 17 with with IN cord-022235-6ircruag 381 18 anti anti JJ cord-022235-6ircruag 381 19 - - JJ cord-022235-6ircruag 381 20 HA HA NNP cord-022235-6ircruag 381 21 antibodies antibody NNS cord-022235-6ircruag 381 22 applied apply VBD cord-022235-6ircruag 381 23 to to IN cord-022235-6ircruag 381 24 the the DT cord-022235-6ircruag 381 25 basal basal NN cord-022235-6ircruag 381 26 surface surface NN cord-022235-6ircruag 381 27 of of IN cord-022235-6ircruag 381 28 MDCK MDCK NNP cord-022235-6ircruag 381 29 cells cell NNS cord-022235-6ircruag 381 30 led lead VBD cord-022235-6ircruag 381 31 Matlin Matlin NNP cord-022235-6ircruag 381 32 and and CC cord-022235-6ircruag 381 33 Simons Simons NNP cord-022235-6ircruag 381 34 ( ( -LRB- cord-022235-6ircruag 381 35 695 695 CD cord-022235-6ircruag 381 36 ) ) -RRB- cord-022235-6ircruag 381 37 to to TO cord-022235-6ircruag 381 38 conclude conclude VB cord-022235-6ircruag 381 39 that that IN cord-022235-6ircruag 381 40 HA HA NNP cord-022235-6ircruag 381 41 was be VBD cord-022235-6ircruag 381 42 targeted target VBN cord-022235-6ircruag 381 43 directly directly RB cord-022235-6ircruag 381 44 to to IN cord-022235-6ircruag 381 45 the the DT cord-022235-6ircruag 381 46 apical apical JJ cord-022235-6ircruag 381 47 surface surface NN cord-022235-6ircruag 381 48 . . . cord-022235-6ircruag 382 1 Conversely conversely RB cord-022235-6ircruag 382 2 , , , cord-022235-6ircruag 382 3 Pfeffer Pfeffer NNP cord-022235-6ircruag 382 4 et et NNP cord-022235-6ircruag 382 5 al al NNP cord-022235-6ircruag 382 6 . . . cord-022235-6ircruag 383 1 ( ( -LRB- cord-022235-6ircruag 383 2 873 873 CD cord-022235-6ircruag 383 3 ) ) -RRB- cord-022235-6ircruag 383 4 showed show VBD cord-022235-6ircruag 383 5 that that IN cord-022235-6ircruag 383 6 pulse pulse NN cord-022235-6ircruag 383 7 - - HYPH cord-022235-6ircruag 383 8 labeled label VBN cord-022235-6ircruag 383 9 G g NN cord-022235-6ircruag 383 10 protein protein NN cord-022235-6ircruag 383 11 went go VBD cord-022235-6ircruag 383 12 directly directly RB cord-022235-6ircruag 383 13 to to IN cord-022235-6ircruag 383 14 the the DT cord-022235-6ircruag 383 15 basolateral basolateral JJ cord-022235-6ircruag 383 16 mem mem NN cord-022235-6ircruag 383 17 brane brane NN cord-022235-6ircruag 383 18 , , , cord-022235-6ircruag 383 19 where where WRB cord-022235-6ircruag 383 20 it -PRON- PRP cord-022235-6ircruag 383 21 could could MD cord-022235-6ircruag 383 22 be be VB cord-022235-6ircruag 383 23 detected detect VBN cord-022235-6ircruag 383 24 with with IN cord-022235-6ircruag 383 25 specific specific JJ cord-022235-6ircruag 383 26 monoclonal monoclonal JJ cord-022235-6ircruag 383 27 antibodies antibody NNS cord-022235-6ircruag 383 28 . . . cord-022235-6ircruag 384 1 Another another DT cord-022235-6ircruag 384 2 approach approach NN cord-022235-6ircruag 384 3 to to IN cord-022235-6ircruag 384 4 studying study VBG cord-022235-6ircruag 384 5 the the DT cord-022235-6ircruag 384 6 sorting sorting NN cord-022235-6ircruag 384 7 of of IN cord-022235-6ircruag 384 8 HA HA NNP cord-022235-6ircruag 384 9 and and CC cord-022235-6ircruag 384 10 G g NN cord-022235-6ircruag 384 11 proteins protein NNS cord-022235-6ircruag 384 12 is be VBZ cord-022235-6ircruag 384 13 to to TO cord-022235-6ircruag 384 14 ' ' '' cord-022235-6ircruag 384 15 ' ' `` cord-022235-6ircruag 384 16 freeze freeze VB cord-022235-6ircruag 384 17 " " '' cord-022235-6ircruag 384 18 them -PRON- PRP cord-022235-6ircruag 384 19 in in IN cord-022235-6ircruag 384 20 the the DT cord-022235-6ircruag 384 21 Golgi Golgi NNP cord-022235-6ircruag 384 22 apparatus apparatus NN cord-022235-6ircruag 384 23 by by IN cord-022235-6ircruag 384 24 cooling cool VBG cord-022235-6ircruag 384 25 the the DT cord-022235-6ircruag 384 26 cells cell NNS cord-022235-6ircruag 384 27 to to IN cord-022235-6ircruag 384 28 20 20 CD cord-022235-6ircruag 384 29 ° ° NN cord-022235-6ircruag 384 30 C c NN cord-022235-6ircruag 384 31 ( ( -LRB- cord-022235-6ircruag 384 32 which which WDT cord-022235-6ircruag 384 33 specifically specifically RB cord-022235-6ircruag 384 34 blocks block VBZ cord-022235-6ircruag 384 35 protein protein NN cord-022235-6ircruag 384 36 exit exit NN cord-022235-6ircruag 384 37 from from IN cord-022235-6ircruag 384 38 the the DT cord-022235-6ircruag 384 39 TGN TGN NNP cord-022235-6ircruag 384 40 ) ) -RRB- cord-022235-6ircruag 384 41 or or CC cord-022235-6ircruag 384 42 to to TO cord-022235-6ircruag 384 43 use use VB cord-022235-6ircruag 384 44 cells cell NNS cord-022235-6ircruag 384 45 infected infect VBN cord-022235-6ircruag 384 46 with with IN cord-022235-6ircruag 384 47 viruses virus NNS cord-022235-6ircruag 384 48 carrying carry VBG cord-022235-6ircruag 384 49 temperature temperature NN cord-022235-6ircruag 384 50 - - HYPH cord-022235-6ircruag 384 51 sensitive sensitive JJ cord-022235-6ircruag 384 52 mutations mutation NNS cord-022235-6ircruag 384 53 in in IN cord-022235-6ircruag 384 54 the the DT cord-022235-6ircruag 384 55 HA HA NNP cord-022235-6ircruag 384 56 or or CC cord-022235-6ircruag 384 57 G g NN cord-022235-6ircruag 384 58 genes gene NNS cord-022235-6ircruag 384 59 at at IN cord-022235-6ircruag 384 60 the the DT cord-022235-6ircruag 384 61 restrictive restrictive JJ cord-022235-6ircruag 384 62 temperature temperature NN cord-022235-6ircruag 384 63 . . . cord-022235-6ircruag 385 1 The the DT cord-022235-6ircruag 385 2 bulk bulk JJ cord-022235-6ircruag 385 3 movement movement NN cord-022235-6ircruag 385 4 of of IN cord-022235-6ircruag 385 5 these these DT cord-022235-6ircruag 385 6 proteins protein NNS cord-022235-6ircruag 385 7 can can MD cord-022235-6ircruag 385 8 thus thus RB cord-022235-6ircruag 385 9 be be VB cord-022235-6ircruag 385 10 followed follow VBN cord-022235-6ircruag 385 11 by by IN cord-022235-6ircruag 385 12 immunocytochemistry immunocytochemistry NN cord-022235-6ircruag 385 13 when when WRB cord-022235-6ircruag 385 14 the the DT cord-022235-6ircruag 385 15 cells cell NNS cord-022235-6ircruag 385 16 are be VBP cord-022235-6ircruag 385 17 restored restore VBN cord-022235-6ircruag 385 18 to to IN cord-022235-6ircruag 385 19 the the DT cord-022235-6ircruag 385 20 permissive permissive JJ cord-022235-6ircruag 385 21 temperature temperature NN cord-022235-6ircruag 385 22 . . . cord-022235-6ircruag 386 1 Rindler Rindler NNP cord-022235-6ircruag 386 2 et et NNP cord-022235-6ircruag 386 3 al al NNP cord-022235-6ircruag 386 4 . . . cord-022235-6ircruag 387 1 ( ( -LRB- cord-022235-6ircruag 387 2 929 929 CD cord-022235-6ircruag 387 3 ) ) -RRB- cord-022235-6ircruag 387 4 found find VBD cord-022235-6ircruag 387 5 that that IN cord-022235-6ircruag 387 6 Gts Gts NNP cord-022235-6ircruag 387 7 protein protein NN cord-022235-6ircruag 387 8 was be VBD cord-022235-6ircruag 387 9 transported transport VBN cord-022235-6ircruag 387 10 directly directly RB cord-022235-6ircruag 387 11 from from IN cord-022235-6ircruag 387 12 the the DT cord-022235-6ircruag 387 13 Golgi Golgi NNP cord-022235-6ircruag 387 14 to to IN cord-022235-6ircruag 387 15 the the DT cord-022235-6ircruag 387 16 adjacent adjacent JJ cord-022235-6ircruag 387 17 lateral lateral JJ cord-022235-6ircruag 387 18 cell cell NN cord-022235-6ircruag 387 19 sur sur FW cord-022235-6ircruag 387 20 face face NN cord-022235-6ircruag 387 21 , , , cord-022235-6ircruag 387 22 whereas whereas IN cord-022235-6ircruag 387 23 wild wild JJ cord-022235-6ircruag 387 24 - - HYPH cord-022235-6ircruag 387 25 type type NN cord-022235-6ircruag 387 26 HA HA NNP cord-022235-6ircruag 387 27 accumulated accumulate VBN cord-022235-6ircruag 387 28 at at IN cord-022235-6ircruag 387 29 20 20 CD cord-022235-6ircruag 387 30 ° ° NN cord-022235-6ircruag 387 31 C C NNP cord-022235-6ircruag 387 32 was be VBD cord-022235-6ircruag 387 33 sorted sort VBN cord-022235-6ircruag 387 34 directly directly RB cord-022235-6ircruag 387 35 to to IN cord-022235-6ircruag 387 36 the the DT cord-022235-6ircruag 387 37 region region NN cord-022235-6ircruag 387 38 of of IN cord-022235-6ircruag 387 39 the the DT cord-022235-6ircruag 387 40 apical apical JJ cord-022235-6ircruag 387 41 surface surface NN cord-022235-6ircruag 387 42 closest close JJS cord-022235-6ircruag 387 43 to to IN cord-022235-6ircruag 387 44 the the DT cord-022235-6ircruag 387 45 TGN TGN NNP cord-022235-6ircruag 387 46 . . . cord-022235-6ircruag 388 1 Similarly similarly RB cord-022235-6ircruag 388 2 , , , cord-022235-6ircruag 388 3 Pfeiffer Pfeiffer NNP cord-022235-6ircruag 388 4 et et NNP cord-022235-6ircruag 388 5 al al NNP cord-022235-6ircruag 388 6 . . . cord-022235-6ircruag 389 1 ( ( -LRB- cord-022235-6ircruag 389 2 873 873 CD cord-022235-6ircruag 389 3 ) ) -RRB- cord-022235-6ircruag 389 4 found find VBD cord-022235-6ircruag 389 5 that that IN cord-022235-6ircruag 389 6 G g NN cord-022235-6ircruag 389 7 protein protein NN cord-022235-6ircruag 389 8 accumulated accumulate VBN cord-022235-6ircruag 389 9 in in IN cord-022235-6ircruag 389 10 the the DT cord-022235-6ircruag 389 11 TGN TGN NNP cord-022235-6ircruag 389 12 went go VBD cord-022235-6ircruag 389 13 to to IN cord-022235-6ircruag 389 14 the the DT cord-022235-6ircruag 389 15 baso baso NNP cord-022235-6ircruag 389 16 lateral lateral JJ cord-022235-6ircruag 389 17 membrane membrane NN cord-022235-6ircruag 389 18 67 67 CD cord-022235-6ircruag 389 19 times time NNS cord-022235-6ircruag 389 20 faster fast RBR cord-022235-6ircruag 389 21 than than IN cord-022235-6ircruag 389 22 to to IN cord-022235-6ircruag 389 23 the the DT cord-022235-6ircruag 389 24 apical apical JJ cord-022235-6ircruag 389 25 surface surface NN cord-022235-6ircruag 389 26 when when WRB cord-022235-6ircruag 389 27 the the DT cord-022235-6ircruag 389 28 cells cell NNS cord-022235-6ircruag 389 29 were be VBD cord-022235-6ircruag 389 30 warmed warm VBN cord-022235-6ircruag 389 31 to to IN cord-022235-6ircruag 389 32 37 37 CD cord-022235-6ircruag 389 33 ° ° NN cord-022235-6ircruag 389 34 C c NN cord-022235-6ircruag 389 35 . . . cord-022235-6ircruag 390 1 These these DT cord-022235-6ircruag 390 2 studies study NNS cord-022235-6ircruag 390 3 are be VBP cord-022235-6ircruag 390 4 compatible compatible JJ cord-022235-6ircruag 390 5 with with IN cord-022235-6ircruag 390 6 the the DT cord-022235-6ircruag 390 7 idea idea NN cord-022235-6ircruag 390 8 that that IN cord-022235-6ircruag 390 9 apical apical JJ cord-022235-6ircruag 390 10 and and CC cord-022235-6ircruag 390 11 basolateral basolateral JJ cord-022235-6ircruag 390 12 proteins protein NNS cord-022235-6ircruag 390 13 go go VBP cord-022235-6ircruag 390 14 directly directly RB cord-022235-6ircruag 390 15 to to IN cord-022235-6ircruag 390 16 their -PRON- PRP$ cord-022235-6ircruag 390 17 respective respective JJ cord-022235-6ircruag 390 18 target target NN cord-022235-6ircruag 390 19 mem mem NNS cord-022235-6ircruag 390 20 branes brane NNS cord-022235-6ircruag 390 21 . . . cord-022235-6ircruag 391 1 Different different JJ cord-022235-6ircruag 391 2 results result NNS cord-022235-6ircruag 391 3 were be VBD cord-022235-6ircruag 391 4 reported report VBN cord-022235-6ircruag 391 5 by by IN cord-022235-6ircruag 391 6 Bartles Bartles NNP cord-022235-6ircruag 391 7 et et NNP cord-022235-6ircruag 391 8 al al NNP cord-022235-6ircruag 391 9 . . . cord-022235-6ircruag 392 1 ( ( -LRB- cord-022235-6ircruag 392 2 50 50 CD cord-022235-6ircruag 392 3 ) ) -RRB- cord-022235-6ircruag 392 4 , , , cord-022235-6ircruag 392 5 who who WP cord-022235-6ircruag 392 6 found find VBD cord-022235-6ircruag 392 7 that that IN cord-022235-6ircruag 392 8 endogenous endogenous JJ cord-022235-6ircruag 392 9 apical apical JJ cord-022235-6ircruag 392 10 proteins protein NNS cord-022235-6ircruag 392 11 in in IN cord-022235-6ircruag 392 12 hepatocytes hepatocyte NNS cord-022235-6ircruag 392 13 appeared appear VBD cord-022235-6ircruag 392 14 first first RB cord-022235-6ircruag 392 15 in in IN cord-022235-6ircruag 392 16 the the DT cord-022235-6ircruag 392 17 baso baso NNP cord-022235-6ircruag 392 18 lateral lateral JJ cord-022235-6ircruag 392 19 membrane membrane NN cord-022235-6ircruag 392 20 and and CC cord-022235-6ircruag 392 21 were be VBD cord-022235-6ircruag 392 22 subsequently subsequently RB cord-022235-6ircruag 392 23 redistributed redistribute VBN cord-022235-6ircruag 392 24 to to IN cord-022235-6ircruag 392 25 the the DT cord-022235-6ircruag 392 26 apical apical JJ cord-022235-6ircruag 392 27 sur sur FW cord-022235-6ircruag 392 28 face face NN cord-022235-6ircruag 392 29 by by IN cord-022235-6ircruag 392 30 transcytosis transcytosis NNP cord-022235-6ircruag 392 31 ( ( -LRB- cord-022235-6ircruag 392 32 Section section NN cord-022235-6ircruag 392 33 VIII.A.5.b VIII.A.5.b NNP cord-022235-6ircruag 392 34 ) ) -RRB- cord-022235-6ircruag 392 35 . . . cord-022235-6ircruag 393 1 Whether whether IN cord-022235-6ircruag 393 2 this this DT cord-022235-6ircruag 393 3 result result NN cord-022235-6ircruag 393 4 indicates indicate VBZ cord-022235-6ircruag 393 5 the the DT cord-022235-6ircruag 393 6 existence existence NN cord-022235-6ircruag 393 7 of of IN cord-022235-6ircruag 393 8 totally totally RB cord-022235-6ircruag 393 9 different different JJ cord-022235-6ircruag 393 10 mechanisms mechanism NNS cord-022235-6ircruag 393 11 for for IN cord-022235-6ircruag 393 12 sorting sort VBG cord-022235-6ircruag 393 13 apical apical JJ cord-022235-6ircruag 393 14 proteins protein NNS cord-022235-6ircruag 393 15 in in IN cord-022235-6ircruag 393 16 kidney kidney NN cord-022235-6ircruag 393 17 and and CC cord-022235-6ircruag 393 18 liver liver NN cord-022235-6ircruag 393 19 cells cell NNS cord-022235-6ircruag 393 20 could could MD cord-022235-6ircruag 393 21 be be VB cord-022235-6ircruag 393 22 tested test VBN cord-022235-6ircruag 393 23 by by IN cord-022235-6ircruag 393 24 expressing express VBG cord-022235-6ircruag 393 25 hepatocyte hepatocyte NN cord-022235-6ircruag 393 26 genes gene NNS cord-022235-6ircruag 393 27 for for IN cord-022235-6ircruag 393 28 apical apical JJ cord-022235-6ircruag 393 29 proteins protein NNS cord-022235-6ircruag 393 30 in in IN cord-022235-6ircruag 393 31 MDCK MDCK NNP cord-022235-6ircruag 393 32 cells cell NNS cord-022235-6ircruag 393 33 . . . cord-022235-6ircruag 394 1 Sorting sort VBG cord-022235-6ircruag 394 2 of of IN cord-022235-6ircruag 394 3 polarized polarized JJ cord-022235-6ircruag 394 4 secreted secrete VBN cord-022235-6ircruag 394 5 and and CC cord-022235-6ircruag 394 6 membrane membrane NN cord-022235-6ircruag 394 7 proteins protein NNS cord-022235-6ircruag 394 8 is be VBZ cord-022235-6ircruag 394 9 assumed assume VBN cord-022235-6ircruag 394 10 to to IN cord-022235-6ircruag 394 11 de de NNP cord-022235-6ircruag 394 12 pend pend VB cord-022235-6ircruag 394 13 on on IN cord-022235-6ircruag 394 14 sorting sorting NN cord-022235-6ircruag 394 15 signals signal NNS cord-022235-6ircruag 394 16 ( ( -LRB- cord-022235-6ircruag 394 17 probably probably RB cord-022235-6ircruag 394 18 signal signal VBP cord-022235-6ircruag 394 19 patches patch NNS cord-022235-6ircruag 394 20 ) ) -RRB- cord-022235-6ircruag 395 1 [ [ -LRB- cord-022235-6ircruag 395 2 see see UH cord-022235-6ircruag 395 3 Section Section NNP cord-022235-6ircruag 395 4 I.D.2 I.D.2 NNP cord-022235-6ircruag 395 5 and and CC cord-022235-6ircruag 395 6 ( ( -LRB- cord-022235-6ircruag 395 7 88 88 CD cord-022235-6ircruag 395 8 ) ) -RRB- cord-022235-6ircruag 395 9 ] ] -RRB- cord-022235-6ircruag 396 1 present present JJ cord-022235-6ircruag 396 2 in in IN cord-022235-6ircruag 396 3 the the DT cord-022235-6ircruag 396 4 sorted sort VBN cord-022235-6ircruag 396 5 proteins protein NNS cord-022235-6ircruag 396 6 and and CC cord-022235-6ircruag 396 7 on on IN cord-022235-6ircruag 396 8 their -PRON- PRP$ cord-022235-6ircruag 396 9 interaction interaction NN cord-022235-6ircruag 396 10 with with IN cord-022235-6ircruag 396 11 receptors receptor NNS cord-022235-6ircruag 396 12 . . . cord-022235-6ircruag 397 1 Sorting sort VBG cord-022235-6ircruag 397 2 presumably presumably RB cord-022235-6ircruag 397 3 occurs occur VBZ cord-022235-6ircruag 397 4 in in IN cord-022235-6ircruag 397 5 the the DT cord-022235-6ircruag 397 6 TGN TGN NNP cord-022235-6ircruag 397 7 , , , cord-022235-6ircruag 397 8 where where WRB cord-022235-6ircruag 397 9 at at RB cord-022235-6ircruag 397 10 least least RBS cord-022235-6ircruag 397 11 one one CD cord-022235-6ircruag 397 12 set set NN cord-022235-6ircruag 397 13 of of IN cord-022235-6ircruag 397 14 cognate cognate JJ cord-022235-6ircruag 397 15 receptors receptor NNS cord-022235-6ircruag 397 16 are be VBP cord-022235-6ircruag 397 17 probably probably RB cord-022235-6ircruag 397 18 located locate VBN cord-022235-6ircruag 397 19 . . . cord-022235-6ircruag 398 1 To to IN cord-022235-6ircruag 398 2 date date NN cord-022235-6ircruag 398 3 , , , cord-022235-6ircruag 398 4 the the DT cord-022235-6ircruag 398 5 primary primary JJ cord-022235-6ircruag 398 6 approach approach NN cord-022235-6ircruag 398 7 used use VBD cord-022235-6ircruag 398 8 to to TO cord-022235-6ircruag 398 9 locate locate VB cord-022235-6ircruag 398 10 sorting sorting NN cord-022235-6ircruag 398 11 signals signal NNS cord-022235-6ircruag 398 12 has have VBZ cord-022235-6ircruag 398 13 been be VBN cord-022235-6ircruag 398 14 to to TO cord-022235-6ircruag 398 15 introduce introduce VB cord-022235-6ircruag 398 16 major major JJ cord-022235-6ircruag 398 17 sequence sequence NN cord-022235-6ircruag 398 18 alterations alteration NNS cord-022235-6ircruag 398 19 including include VBG cord-022235-6ircruag 398 20 the the DT cord-022235-6ircruag 398 21 deletion deletion NN cord-022235-6ircruag 398 22 of of IN cord-022235-6ircruag 398 23 cytoplasmic cytoplasmic JJ cord-022235-6ircruag 398 24 or or CC cord-022235-6ircruag 398 25 transmembrane transmembrane JJ cord-022235-6ircruag 398 26 domains domain NNS cord-022235-6ircruag 398 27 from from IN cord-022235-6ircruag 398 28 the the DT cord-022235-6ircruag 398 29 HA HA NNP cord-022235-6ircruag 398 30 , , , cord-022235-6ircruag 398 31 G g NN cord-022235-6ircruag 398 32 , , , cord-022235-6ircruag 398 33 or or CC cord-022235-6ircruag 398 34 other other JJ cord-022235-6ircruag 398 35 polarized polarized JJ cord-022235-6ircruag 398 36 viral viral JJ cord-022235-6ircruag 398 37 glycoproteins glycoprotein NNS cord-022235-6ircruag 398 38 , , , cord-022235-6ircruag 398 39 or or CC cord-022235-6ircruag 398 40 to to TO cord-022235-6ircruag 398 41 create create VB cord-022235-6ircruag 398 42 hybrids hybrid NNS cord-022235-6ircruag 398 43 be be VB cord-022235-6ircruag 398 44 tween tween VBG cord-022235-6ircruag 398 45 them -PRON- PRP cord-022235-6ircruag 398 46 . . . cord-022235-6ircruag 399 1 Even even RB cord-022235-6ircruag 399 2 these these DT cord-022235-6ircruag 399 3 relatively relatively RB cord-022235-6ircruag 399 4 unsophisticated unsophisticated JJ cord-022235-6ircruag 399 5 studies study NNS cord-022235-6ircruag 399 6 give give VBP cord-022235-6ircruag 399 7 conflicting conflicting JJ cord-022235-6ircruag 399 8 results result NNS cord-022235-6ircruag 399 9 . . . cord-022235-6ircruag 400 1 For for IN cord-022235-6ircruag 400 2 example example NN cord-022235-6ircruag 400 3 , , , cord-022235-6ircruag 400 4 McQueen McQueen NNP cord-022235-6ircruag 400 5 et et NNP cord-022235-6ircruag 400 6 al al NNP cord-022235-6ircruag 400 7 . . . cord-022235-6ircruag 400 8 ( ( -LRB- cord-022235-6ircruag 400 9 702 702 CD cord-022235-6ircruag 400 10 ) ) -RRB- cord-022235-6ircruag 400 11 and and CC cord-022235-6ircruag 400 12 Roth Roth NNP cord-022235-6ircruag 400 13 et et FW cord-022235-6ircruag 400 14 al al NNP cord-022235-6ircruag 400 15 . . . cord-022235-6ircruag 401 1 ( ( -LRB- cord-022235-6ircruag 401 2 959 959 CD cord-022235-6ircruag 401 3 ) ) -RRB- cord-022235-6ircruag 401 4 found find VBD cord-022235-6ircruag 401 5 that that IN cord-022235-6ircruag 401 6 either either CC cord-022235-6ircruag 401 7 deleting delete VBG cord-022235-6ircruag 401 8 the the DT cord-022235-6ircruag 401 9 transmembranous transmembranous JJ cord-022235-6ircruag 401 10 and and CC cord-022235-6ircruag 401 11 cytoplasmic cytoplasmic JJ cord-022235-6ircruag 401 12 tail tail NN cord-022235-6ircruag 401 13 of of IN cord-022235-6ircruag 401 14 HA HA NNP cord-022235-6ircruag 401 15 ( ( -LRB- cord-022235-6ircruag 401 16 to to TO cord-022235-6ircruag 401 17 make make VB cord-022235-6ircruag 401 18 a a DT cord-022235-6ircruag 401 19 soluble soluble JJ cord-022235-6ircruag 401 20 , , , cord-022235-6ircruag 401 21 secreted secrete VBN cord-022235-6ircruag 401 22 protein protein NN cord-022235-6ircruag 401 23 ) ) -RRB- cord-022235-6ircruag 401 24 or or CC cord-022235-6ircruag 401 25 replacing replace VBG cord-022235-6ircruag 401 26 them -PRON- PRP cord-022235-6ircruag 401 27 with with IN cord-022235-6ircruag 401 28 the the DT cord-022235-6ircruag 401 29 correspond correspond NN cord-022235-6ircruag 401 30 ing e VBG cord-022235-6ircruag 401 31 regions region NNS cord-022235-6ircruag 401 32 of of IN cord-022235-6ircruag 401 33 G G NNP cord-022235-6ircruag 401 34 did do VBD cord-022235-6ircruag 401 35 not not RB cord-022235-6ircruag 401 36 affect affect VB cord-022235-6ircruag 401 37 apical apical JJ cord-022235-6ircruag 401 38 targeting targeting NN cord-022235-6ircruag 401 39 and and CC cord-022235-6ircruag 401 40 concluded conclude VBD cord-022235-6ircruag 401 41 that that IN cord-022235-6ircruag 401 42 the the DT cord-022235-6ircruag 401 43 apical apical JJ cord-022235-6ircruag 401 44 sorting sorting NN cord-022235-6ircruag 401 45 signal signal NN cord-022235-6ircruag 401 46 was be VBD cord-022235-6ircruag 401 47 located locate VBN cord-022235-6ircruag 401 48 in in IN cord-022235-6ircruag 401 49 the the DT cord-022235-6ircruag 401 50 N n CD cord-022235-6ircruag 401 51 - - HYPH cord-022235-6ircruag 401 52 terminal terminal NN cord-022235-6ircruag 401 53 , , , cord-022235-6ircruag 401 54 extracytoplasmic extracytoplasmic NNP cord-022235-6ircruag 401 55 do do VBP cord-022235-6ircruag 401 56 main main NN cord-022235-6ircruag 401 57 of of IN cord-022235-6ircruag 401 58 HA HA NNP cord-022235-6ircruag 401 59 . . . cord-022235-6ircruag 402 1 Gonzalez Gonzalez NNP cord-022235-6ircruag 402 2 et et NNP cord-022235-6ircruag 402 3 al al NNP cord-022235-6ircruag 402 4 . . . cord-022235-6ircruag 403 1 ( ( -LRB- cord-022235-6ircruag 403 2 387 387 CD cord-022235-6ircruag 403 3 ) ) -RRB- cord-022235-6ircruag 403 4 reported report VBN cord-022235-6ircruag 403 5 , , , cord-022235-6ircruag 403 6 however however RB cord-022235-6ircruag 403 7 , , , cord-022235-6ircruag 403 8 that that DT cord-022235-6ircruag 403 9 truncated truncate VBD cord-022235-6ircruag 403 10 HA HA NNP cord-022235-6ircruag 403 11 was be VBD cord-022235-6ircruag 403 12 secreted secrete VBN cord-022235-6ircruag 403 13 by by IN cord-022235-6ircruag 403 14 the the DT cord-022235-6ircruag 403 15 default default NN cord-022235-6ircruag 403 16 pathway pathway NN cord-022235-6ircruag 403 17 and and CC cord-022235-6ircruag 403 18 concluded conclude VBD cord-022235-6ircruag 403 19 that that IN cord-022235-6ircruag 403 20 the the DT cord-022235-6ircruag 403 21 sorting sorting NN cord-022235-6ircruag 403 22 signal signal NN cord-022235-6ircruag 403 23 was be VBD cord-022235-6ircruag 403 24 in in IN cord-022235-6ircruag 403 25 the the DT cord-022235-6ircruag 403 26 C C NNP cord-022235-6ircruag 403 27 - - HYPH cord-022235-6ircruag 403 28 terminal terminal NN cord-022235-6ircruag 403 29 transmembranous transmembranous NNP cord-022235-6ircruag 403 30 or or CC cord-022235-6ircruag 403 31 10-amino 10-amino CD cord-022235-6ircruag 403 32 - - HYPH cord-022235-6ircruag 403 33 acid acid NN cord-022235-6ircruag 403 34 cytoplasmic cytoplasmic JJ cord-022235-6ircruag 403 35 domains domain NNS cord-022235-6ircruag 403 36 . . . cord-022235-6ircruag 404 1 There there EX cord-022235-6ircruag 404 2 is be VBZ cord-022235-6ircruag 404 3 also also RB cord-022235-6ircruag 404 4 disagreement disagreement NN cord-022235-6ircruag 404 5 concerning concern VBG cord-022235-6ircruag 404 6 the the DT cord-022235-6ircruag 404 7 location location NN cord-022235-6ircruag 404 8 of of IN cord-022235-6ircruag 404 9 the the DT cord-022235-6ircruag 404 10 G g NN cord-022235-6ircruag 404 11 protein protein NN cord-022235-6ircruag 404 12 basolateral basolateral JJ cord-022235-6ircruag 404 13 sorting sorting NN cord-022235-6ircruag 404 14 signal signal NN cord-022235-6ircruag 404 15 , , , cord-022235-6ircruag 404 16 which which WDT cord-022235-6ircruag 404 17 , , , cord-022235-6ircruag 404 18 according accord VBG cord-022235-6ircruag 404 19 to to IN cord-022235-6ircruag 404 20 the the DT cord-022235-6ircruag 404 21 deletion deletion NN cord-022235-6ircruag 404 22 and and CC cord-022235-6ircruag 404 23 HA HA NNP cord-022235-6ircruag 404 24 gene gene NN cord-022235-6ircruag 404 25 fusion fusion NN cord-022235-6ircruag 404 26 studies study NNS cord-022235-6ircruag 404 27 of of IN cord-022235-6ircruag 404 28 Paddington Paddington NNP cord-022235-6ircruag 404 29 et et NNP cord-022235-6ircruag 404 30 al al NNP cord-022235-6ircruag 404 31 . . . cord-022235-6ircruag 405 1 ( ( -LRB- cord-022235-6ircruag 405 2 835 835 CD cord-022235-6ircruag 405 3 ) ) -RRB- cord-022235-6ircruag 405 4 and and CC cord-022235-6ircruag 405 5 Gonzalez Gonzalez NNP cord-022235-6ircruag 405 6 et et NNP cord-022235-6ircruag 405 7 al al NNP cord-022235-6ircruag 405 8 . . . cord-022235-6ircruag 406 1 ( ( -LRB- cord-022235-6ircruag 406 2 387 387 CD cord-022235-6ircruag 406 3 ) ) -RRB- cord-022235-6ircruag 406 4 is be VBZ cord-022235-6ircruag 406 5 located locate VBN cord-022235-6ircruag 406 6 in in IN cord-022235-6ircruag 406 7 the the DT cord-022235-6ircruag 406 8 29-amino 29-amino CD cord-022235-6ircruag 406 9 - - HYPH cord-022235-6ircruag 406 10 acid acid NN cord-022235-6ircruag 406 11 , , , cord-022235-6ircruag 406 12 C c NN cord-022235-6ircruag 406 13 - - HYPH cord-022235-6ircruag 406 14 terminal terminal JJ cord-022235-6ircruag 406 15 cytoplasmic cytoplasmic JJ cord-022235-6ircruag 406 16 domain domain NN cord-022235-6ircruag 406 17 . . . cord-022235-6ircruag 407 1 Stephens Stephens NNP cord-022235-6ircruag 407 2 and and CC cord-022235-6ircruag 407 3 Compans Compans NNP cord-022235-6ircruag 407 4 ( ( -LRB- cord-022235-6ircruag 407 5 1080 1080 CD cord-022235-6ircruag 407 6 ) ) -RRB- cord-022235-6ircruag 407 7 McQueen McQueen NNP cord-022235-6ircruag 407 8 et et NNP cord-022235-6ircruag 407 9 al al NNP cord-022235-6ircruag 407 10 . . . cord-022235-6ircruag 408 1 ( ( -LRB- cord-022235-6ircruag 408 2 703 703 CD cord-022235-6ircruag 408 3 ) ) -RRB- cord-022235-6ircruag 408 4 have have VBP cord-022235-6ircruag 408 5 suggested suggest VBN cord-022235-6ircruag 408 6 that that IN cord-022235-6ircruag 408 7 the the DT cord-022235-6ircruag 408 8 origin origin NN cord-022235-6ircruag 408 9 of of IN cord-022235-6ircruag 408 10 these these DT cord-022235-6ircruag 408 11 conflicting conflict VBG cord-022235-6ircruag 408 12 results result NNS cord-022235-6ircruag 408 13 may may MD cord-022235-6ircruag 408 14 be be VB cord-022235-6ircruag 408 15 the the DT cord-022235-6ircruag 408 16 fact fact NN cord-022235-6ircruag 408 17 that that IN cord-022235-6ircruag 408 18 recombinant recombinant JJ cord-022235-6ircruag 408 19 genes gene NNS cord-022235-6ircruag 408 20 are be VBP cord-022235-6ircruag 408 21 not not RB cord-022235-6ircruag 408 22 stably stably RB cord-022235-6ircruag 408 23 expressed express VBN cord-022235-6ircruag 408 24 in in IN cord-022235-6ircruag 408 25 MDCK MDCK NNP cord-022235-6ircruag 408 26 cells cell NNS cord-022235-6ircruag 408 27 . . . cord-022235-6ircruag 409 1 Their -PRON- PRP$ cord-022235-6ircruag 409 2 studies study NNS cord-022235-6ircruag 409 3 ( ( -LRB- cord-022235-6ircruag 409 4 702,703 702,703 CD cord-022235-6ircruag 409 5 ) ) -RRB- cord-022235-6ircruag 409 6 were be VBD cord-022235-6ircruag 409 7 carried carry VBN cord-022235-6ircruag 409 8 out out RP cord-022235-6ircruag 409 9 shortly shortly RB cord-022235-6ircruag 409 10 after after IN cord-022235-6ircruag 409 11 infec infec NN cord-022235-6ircruag 409 12 tion tion NN cord-022235-6ircruag 409 13 by by IN cord-022235-6ircruag 409 14 recombinant recombinant JJ cord-022235-6ircruag 409 15 viruses virus NNS cord-022235-6ircruag 409 16 and and CC cord-022235-6ircruag 409 17 are be VBP cord-022235-6ircruag 409 18 , , , cord-022235-6ircruag 409 19 they -PRON- PRP cord-022235-6ircruag 409 20 claim claim VBP cord-022235-6ircruag 409 21 , , , cord-022235-6ircruag 409 22 more more RBR cord-022235-6ircruag 409 23 likely likely JJ cord-022235-6ircruag 409 24 to to TO cord-022235-6ircruag 409 25 reflect reflect VB cord-022235-6ircruag 409 26 the the DT cord-022235-6ircruag 409 27 true true JJ cord-022235-6ircruag 409 28 sorting sorting NN cord-022235-6ircruag 409 29 pathway pathway NN cord-022235-6ircruag 409 30 of of IN cord-022235-6ircruag 409 31 the the DT cord-022235-6ircruag 409 32 recombinant recombinant JJ cord-022235-6ircruag 409 33 gene gene NN cord-022235-6ircruag 409 34 product product NN cord-022235-6ircruag 409 35 . . . cord-022235-6ircruag 410 1 Other other JJ cord-022235-6ircruag 410 2 groups group NNS cord-022235-6ircruag 410 3 may may MD cord-022235-6ircruag 410 4 in in IN cord-022235-6ircruag 410 5 fact fact NN cord-022235-6ircruag 410 6 be be VB cord-022235-6ircruag 410 7 studying study VBG cord-022235-6ircruag 410 8 the the DT cord-022235-6ircruag 410 9 sorting sorting NN cord-022235-6ircruag 410 10 of of IN cord-022235-6ircruag 410 11 hybrid hybrid JJ cord-022235-6ircruag 410 12 or or CC cord-022235-6ircruag 410 13 truncated truncated JJ cord-022235-6ircruag 410 14 proteins protein NNS cord-022235-6ircruag 410 15 which which WDT cord-022235-6ircruag 410 16 have have VBP cord-022235-6ircruag 410 17 been be VBN cord-022235-6ircruag 410 18 further further RB cord-022235-6ircruag 410 19 modified modify VBN cord-022235-6ircruag 410 20 by by IN cord-022235-6ircruag 410 21 genetic genetic JJ cord-022235-6ircruag 410 22 selection selection NN cord-022235-6ircruag 410 23 for for IN cord-022235-6ircruag 410 24 more more RBR cord-022235-6ircruag 410 25 stable stable JJ cord-022235-6ircruag 410 26 cell cell NN cord-022235-6ircruag 410 27 lines line NNS cord-022235-6ircruag 410 28 . . . cord-022235-6ircruag 411 1 An an DT cord-022235-6ircruag 411 2 other other JJ cord-022235-6ircruag 411 3 argument argument NN cord-022235-6ircruag 411 4 in in IN cord-022235-6ircruag 411 5 favor favor NN cord-022235-6ircruag 411 6 of of IN cord-022235-6ircruag 411 7 the the DT cord-022235-6ircruag 411 8 idea idea NN cord-022235-6ircruag 411 9 that that IN cord-022235-6ircruag 411 10 sorting sorting NN cord-022235-6ircruag 411 11 signals signal NNS cord-022235-6ircruag 411 12 are be VBP cord-022235-6ircruag 411 13 in in IN cord-022235-6ircruag 411 14 the the DT cord-022235-6ircruag 411 15 extracy extracy NNP cord-022235-6ircruag 411 16 toplasmic toplasmic JJ cord-022235-6ircruag 411 17 domain domain NN cord-022235-6ircruag 411 18 is be VBZ cord-022235-6ircruag 411 19 that that IN cord-022235-6ircruag 411 20 this this DT cord-022235-6ircruag 411 21 part part NN cord-022235-6ircruag 411 22 of of IN cord-022235-6ircruag 411 23 the the DT cord-022235-6ircruag 411 24 signal signal NN cord-022235-6ircruag 411 25 is be VBZ cord-022235-6ircruag 411 26 initially initially RB cord-022235-6ircruag 411 27 localized localize VBN cord-022235-6ircruag 411 28 in in IN cord-022235-6ircruag 411 29 the the DT cord-022235-6ircruag 411 30 lumen lumen NN cord-022235-6ircruag 411 31 of of IN cord-022235-6ircruag 411 32 the the DT cord-022235-6ircruag 411 33 TGN TGN NNP cord-022235-6ircruag 411 34 , , , cord-022235-6ircruag 411 35 where where WRB cord-022235-6ircruag 411 36 sorting sorting NN cord-022235-6ircruag 411 37 is be VBZ cord-022235-6ircruag 411 38 presumably presumably RB cord-022235-6ircruag 411 39 initiated initiate VBN cord-022235-6ircruag 411 40 , , , cord-022235-6ircruag 411 41 and and CC cord-022235-6ircruag 411 42 could could MD cord-022235-6ircruag 411 43 there there EX cord-022235-6ircruag 411 44 fore fore RB cord-022235-6ircruag 411 45 bind bind VB cord-022235-6ircruag 411 46 to to IN cord-022235-6ircruag 411 47 hypothetical hypothetical JJ cord-022235-6ircruag 411 48 sorting sorting NN cord-022235-6ircruag 411 49 receptors receptor NNS cord-022235-6ircruag 411 50 which which WDT cord-022235-6ircruag 411 51 segregate segregate VBP cord-022235-6ircruag 411 52 sorted sorted JJ cord-022235-6ircruag 411 53 pro pro JJ cord-022235-6ircruag 411 54 teins tein NNS cord-022235-6ircruag 411 55 from from IN cord-022235-6ircruag 411 56 those those DT cord-022235-6ircruag 411 57 destined destine VBN cord-022235-6ircruag 411 58 for for IN cord-022235-6ircruag 411 59 default default NN cord-022235-6ircruag 411 60 export export NN cord-022235-6ircruag 411 61 or or CC cord-022235-6ircruag 411 62 secretion secretion NN cord-022235-6ircruag 411 63 . . . cord-022235-6ircruag 412 1 These these DT cord-022235-6ircruag 412 2 receptors receptor NNS cord-022235-6ircruag 412 3 may may MD cord-022235-6ircruag 412 4 themselves -PRON- PRP cord-022235-6ircruag 412 5 have have VB cord-022235-6ircruag 412 6 cytoplasmic cytoplasmic JJ cord-022235-6ircruag 412 7 domains domain NNS cord-022235-6ircruag 412 8 recognized recognize VBN cord-022235-6ircruag 412 9 by by IN cord-022235-6ircruag 412 10 receptors receptor NNS cord-022235-6ircruag 412 11 on on IN cord-022235-6ircruag 412 12 the the DT cord-022235-6ircruag 412 13 basolateral basolateral JJ cord-022235-6ircruag 412 14 or or CC cord-022235-6ircruag 412 15 apical apical JJ cord-022235-6ircruag 412 16 surfaces surface NNS cord-022235-6ircruag 412 17 . . . cord-022235-6ircruag 413 1 Furthermore furthermore RB cord-022235-6ircruag 413 2 , , , cord-022235-6ircruag 413 3 such such PDT cord-022235-6ircruag 413 4 a a DT cord-022235-6ircruag 413 5 system system NN cord-022235-6ircruag 413 6 could could MD cord-022235-6ircruag 413 7 han han NNP cord-022235-6ircruag 413 8 dle dle NNP cord-022235-6ircruag 413 9 both both CC cord-022235-6ircruag 413 10 secreted secrete VBD cord-022235-6ircruag 413 11 and and CC cord-022235-6ircruag 413 12 exported export VBN cord-022235-6ircruag 413 13 ( ( -LRB- cord-022235-6ircruag 413 14 membrane membrane NN cord-022235-6ircruag 413 15 ) ) -RRB- cord-022235-6ircruag 413 16 proteins protein NNS cord-022235-6ircruag 413 17 . . . cord-022235-6ircruag 414 1 However however RB cord-022235-6ircruag 414 2 , , , cord-022235-6ircruag 414 3 there there EX cord-022235-6ircruag 414 4 is be VBZ cord-022235-6ircruag 414 5 some some DT cord-022235-6ircruag 414 6 evidence evidence NN cord-022235-6ircruag 414 7 , , , cord-022235-6ircruag 414 8 based base VBN cord-022235-6ircruag 414 9 on on IN cord-022235-6ircruag 414 10 the the DT cord-022235-6ircruag 414 11 effect effect NN cord-022235-6ircruag 414 12 of of IN cord-022235-6ircruag 414 13 NH NH NNP cord-022235-6ircruag 414 14 4 4 CD cord-022235-6ircruag 414 15 C1 C1 NNP cord-022235-6ircruag 414 16 ( ( -LRB- cord-022235-6ircruag 414 17 or or CC cord-022235-6ircruag 414 18 pH pH NNP cord-022235-6ircruag 414 19 ) ) -RRB- cord-022235-6ircruag 414 20 on on IN cord-022235-6ircruag 414 21 basolateral basolateral JJ cord-022235-6ircruag 414 22 sorting sorting NN cord-022235-6ircruag 414 23 of of IN cord-022235-6ircruag 414 24 laminin laminin NN cord-022235-6ircruag 414 25 and and CC cord-022235-6ircruag 414 26 heparin heparin NN cord-022235-6ircruag 414 27 sulfate sulfate NN cord-022235-6ircruag 414 28 proteoglycan proteoglycan NN cord-022235-6ircruag 414 29 , , , cord-022235-6ircruag 414 30 that that DT cord-022235-6ircruag 414 31 soluble soluble JJ cord-022235-6ircruag 414 32 and and CC cord-022235-6ircruag 414 33 membrane membrane NN cord-022235-6ircruag 414 34 proteins protein NNS cord-022235-6ircruag 414 35 may may MD cord-022235-6ircruag 414 36 be be VB cord-022235-6ircruag 414 37 sorted sort VBN cord-022235-6ircruag 414 38 by by IN cord-022235-6ircruag 414 39 different different JJ cord-022235-6ircruag 414 40 mechanisms mechanism NNS cord-022235-6ircruag 414 41 ( ( -LRB- cord-022235-6ircruag 414 42 142 142 CD cord-022235-6ircruag 414 43 ) ) -RRB- cord-022235-6ircruag 414 44 . . . cord-022235-6ircruag 415 1 Clearly clearly RB cord-022235-6ircruag 415 2 , , , cord-022235-6ircruag 415 3 more more JJR cord-022235-6ircruag 415 4 work work NN cord-022235-6ircruag 415 5 remains remain VBZ cord-022235-6ircruag 415 6 to to TO cord-022235-6ircruag 415 7 be be VB cord-022235-6ircruag 415 8 done do VBN cord-022235-6ircruag 415 9 before before IN cord-022235-6ircruag 415 10 we -PRON- PRP cord-022235-6ircruag 415 11 can can MD cord-022235-6ircruag 415 12 identify identify VB cord-022235-6ircruag 415 13 polarity polarity NN cord-022235-6ircruag 415 14 sorting sorting NN cord-022235-6ircruag 415 15 signals signal NNS cord-022235-6ircruag 415 16 with with IN cord-022235-6ircruag 415 17 any any DT cord-022235-6ircruag 415 18 precision precision NN cord-022235-6ircruag 415 19 . . . cord-022235-6ircruag 416 1 One one CD cord-022235-6ircruag 416 2 of of IN cord-022235-6ircruag 416 3 the the DT cord-022235-6ircruag 416 4 difficulties difficulty NNS cord-022235-6ircruag 416 5 may may MD cord-022235-6ircruag 416 6 be be VB cord-022235-6ircruag 416 7 that that IN cord-022235-6ircruag 416 8 these these DT cord-022235-6ircruag 416 9 signals signal NNS cord-022235-6ircruag 416 10 are be VBP cord-022235-6ircruag 416 11 probably probably RB cord-022235-6ircruag 416 12 not not RB cord-022235-6ircruag 416 13 linear linear JJ cord-022235-6ircruag 416 14 sequences sequence NNS cord-022235-6ircruag 416 15 of of IN cord-022235-6ircruag 416 16 amino amino JJ cord-022235-6ircruag 416 17 acids acid NNS cord-022235-6ircruag 416 18 and and CC cord-022235-6ircruag 416 19 are be VBP cord-022235-6ircruag 416 20 therefore therefore RB cord-022235-6ircruag 416 21 less less RBR cord-022235-6ircruag 416 22 amenable amenable JJ cord-022235-6ircruag 416 23 to to IN cord-022235-6ircruag 416 24 gene gene NN cord-022235-6ircruag 416 25 fusion fusion NN cord-022235-6ircruag 416 26 studies study NNS cord-022235-6ircruag 416 27 , , , cord-022235-6ircruag 416 28 which which WDT cord-022235-6ircruag 416 29 have have VBP cord-022235-6ircruag 416 30 been be VBN cord-022235-6ircruag 416 31 so so RB cord-022235-6ircruag 416 32 useful useful JJ cord-022235-6ircruag 416 33 in in IN cord-022235-6ircruag 416 34 identi identi FW cord-022235-6ircruag 416 35 fying fye VBG cord-022235-6ircruag 416 36 routing routing NN cord-022235-6ircruag 416 37 signals signal NNS cord-022235-6ircruag 416 38 . . . cord-022235-6ircruag 417 1 Even even RB cord-022235-6ircruag 417 2 very very RB cord-022235-6ircruag 417 3 subtle subtle JJ cord-022235-6ircruag 417 4 conformational conformational JJ cord-022235-6ircruag 417 5 changes change NNS cord-022235-6ircruag 417 6 may may MD cord-022235-6ircruag 417 7 alter alter VB cord-022235-6ircruag 417 8 sorting sorting NN cord-022235-6ircruag 417 9 signals signal NNS cord-022235-6ircruag 417 10 and and CC cord-022235-6ircruag 417 11 render render VB cord-022235-6ircruag 417 12 them -PRON- PRP cord-022235-6ircruag 417 13 nonfunctional nonfunctional JJ cord-022235-6ircruag 417 14 ; ; : cord-022235-6ircruag 417 15 this this DT cord-022235-6ircruag 417 16 could could MD cord-022235-6ircruag 417 17 explain explain VB cord-022235-6ircruag 417 18 why why WRB cord-022235-6ircruag 417 19 prevention prevention NN cord-022235-6ircruag 417 20 of of IN cord-022235-6ircruag 417 21 glycosylation glycosylation NN cord-022235-6ircruag 417 22 with with IN cord-022235-6ircruag 417 23 tunicamycin tunicamycin NNS cord-022235-6ircruag 417 24 may may MD cord-022235-6ircruag 417 25 cause cause VB cord-022235-6ircruag 417 26 normally normally RB cord-022235-6ircruag 417 27 polar polar JJ cord-022235-6ircruag 417 28 ity ity NN cord-022235-6ircruag 417 29 - - HYPH cord-022235-6ircruag 417 30 sorted sort VBN cord-022235-6ircruag 417 31 proteins protein NNS cord-022235-6ircruag 417 32 to to TO cord-022235-6ircruag 417 33 enter enter VB cord-022235-6ircruag 417 34 the the DT cord-022235-6ircruag 417 35 default default NN cord-022235-6ircruag 417 36 pathway pathway NN cord-022235-6ircruag 417 37 ( ( -LRB- cord-022235-6ircruag 417 38 1135 1135 CD cord-022235-6ircruag 417 39 ) ) -RRB- cord-022235-6ircruag 417 40 . . . cord-022235-6ircruag 418 1 Another another DT cord-022235-6ircruag 418 2 aspect aspect NN cord-022235-6ircruag 418 3 of of IN cord-022235-6ircruag 418 4 the the DT cord-022235-6ircruag 418 5 work work NN cord-022235-6ircruag 418 6 on on IN cord-022235-6ircruag 418 7 polarity polarity NN cord-022235-6ircruag 418 8 sorting sorting NN cord-022235-6ircruag 418 9 signals signal NNS cord-022235-6ircruag 418 10 which which WDT cord-022235-6ircruag 418 11 needs need VBZ cord-022235-6ircruag 418 12 to to TO cord-022235-6ircruag 418 13 be be VB cord-022235-6ircruag 418 14 pursued pursue VBN cord-022235-6ircruag 418 15 is be VBZ cord-022235-6ircruag 418 16 the the DT cord-022235-6ircruag 418 17 identity identity NN cord-022235-6ircruag 418 18 of of IN cord-022235-6ircruag 418 19 receptors receptor NNS cord-022235-6ircruag 418 20 and and CC cord-022235-6ircruag 418 21 the the DT cord-022235-6ircruag 418 22 stage stage NN cord-022235-6ircruag 418 23 at at IN cord-022235-6ircruag 418 24 which which WDT cord-022235-6ircruag 418 25 sorted sort VBD cord-022235-6ircruag 418 26 proteins protein NNS cord-022235-6ircruag 418 27 dissociate dissociate VBP cord-022235-6ircruag 418 28 from from IN cord-022235-6ircruag 418 29 them -PRON- PRP cord-022235-6ircruag 418 30 . . . cord-022235-6ircruag 419 1 Sorting sort VBG cord-022235-6ircruag 419 2 receptors receptor NNS cord-022235-6ircruag 419 3 are be VBP cord-022235-6ircruag 419 4 presumably presumably RB cord-022235-6ircruag 419 5 re re VBG cord-022235-6ircruag 419 6 cycled cycle VBN cord-022235-6ircruag 419 7 . . . cord-022235-6ircruag 420 1 Little little JJ cord-022235-6ircruag 420 2 is be VBZ cord-022235-6ircruag 420 3 known know VBN cord-022235-6ircruag 420 4 about about IN cord-022235-6ircruag 420 5 how how WRB cord-022235-6ircruag 420 6 the the DT cord-022235-6ircruag 420 7 vesicles vesicle NNS cord-022235-6ircruag 420 8 are be VBP cord-022235-6ircruag 420 9 targeted target VBN cord-022235-6ircruag 420 10 to to IN cord-022235-6ircruag 420 11 different different JJ cord-022235-6ircruag 420 12 regions region NNS cord-022235-6ircruag 420 13 of of IN cord-022235-6ircruag 420 14 the the DT cord-022235-6ircruag 420 15 plasma plasma NN cord-022235-6ircruag 420 16 membrane membrane NN cord-022235-6ircruag 420 17 . . . cord-022235-6ircruag 421 1 One one CD cord-022235-6ircruag 421 2 possibility possibility NN cord-022235-6ircruag 421 3 is be VBZ cord-022235-6ircruag 421 4 that that IN cord-022235-6ircruag 421 5 sorting sorting NN cord-022235-6ircruag 421 6 of of IN cord-022235-6ircruag 421 7 lipids lipid NNS cord-022235-6ircruag 421 8 ( ( -LRB- cord-022235-6ircruag 421 9 Fig Fig NNP cord-022235-6ircruag 421 10 . . . cord-022235-6ircruag 421 11 V.5 V.5 NNP cord-022235-6ircruag 421 12 ) ) -RRB- cord-022235-6ircruag 421 13 is be VBZ cord-022235-6ircruag 421 14 involved involve VBN cord-022235-6ircruag 421 15 ( ( -LRB- cord-022235-6ircruag 421 16 709,1050a 709,1050a CD cord-022235-6ircruag 421 17 ) ) -RRB- cord-022235-6ircruag 421 18 . . . cord-022235-6ircruag 422 1 Alternatively alternatively RB cord-022235-6ircruag 422 2 , , , cord-022235-6ircruag 422 3 sorting sort VBG cord-022235-6ircruag 422 4 vesicles vesicle NNS cord-022235-6ircruag 422 5 could could MD cord-022235-6ircruag 422 6 interact interact VB cord-022235-6ircruag 422 7 with with IN cord-022235-6ircruag 422 8 components component NNS cord-022235-6ircruag 422 9 of of IN cord-022235-6ircruag 422 10 the the DT cord-022235-6ircruag 422 11 cytoskeleton cytoskeleton NN cord-022235-6ircruag 422 12 , , , cord-022235-6ircruag 422 13 along along IN cord-022235-6ircruag 422 14 which which WDT cord-022235-6ircruag 422 15 they -PRON- PRP cord-022235-6ircruag 422 16 are be VBP cord-022235-6ircruag 422 17 driven drive VBN cord-022235-6ircruag 422 18 by by IN cord-022235-6ircruag 422 19 ATP ATP NNP cord-022235-6ircruag 422 20 - - HYPH cord-022235-6ircruag 422 21 dependent dependent JJ cord-022235-6ircruag 422 22 motors motor NNS cord-022235-6ircruag 422 23 attached attach VBN cord-022235-6ircruag 422 24 to to IN cord-022235-6ircruag 422 25 microfilaments microfilament NNS cord-022235-6ircruag 422 26 ; ; : cord-022235-6ircruag 422 27 further further JJ cord-022235-6ircruag 422 28 receptor receptor NN cord-022235-6ircruag 422 29 - - HYPH cord-022235-6ircruag 422 30 ligand ligand NN cord-022235-6ircruag 422 31 interactions interaction NNS cord-022235-6ircruag 422 32 might may MD cord-022235-6ircruag 422 33 complete complete VB cord-022235-6ircruag 422 34 the the DT cord-022235-6ircruag 422 35 sorting sorting NN cord-022235-6ircruag 422 36 process process NN cord-022235-6ircruag 422 37 when when WRB cord-022235-6ircruag 422 38 vesi vesi NNP cord-022235-6ircruag 422 39 cles cles NNP cord-022235-6ircruag 422 40 arrive arrive VBP cord-022235-6ircruag 422 41 at at IN cord-022235-6ircruag 422 42 the the DT cord-022235-6ircruag 422 43 plasma plasma NN cord-022235-6ircruag 422 44 membrane membrane NN cord-022235-6ircruag 422 45 ( ( -LRB- cord-022235-6ircruag 422 46 1136 1136 CD cord-022235-6ircruag 422 47 ) ) -RRB- cord-022235-6ircruag 422 48 . . . cord-022235-6ircruag 423 1 Although although IN cord-022235-6ircruag 423 2 there there EX cord-022235-6ircruag 423 3 is be VBZ cord-022235-6ircruag 423 4 no no DT cord-022235-6ircruag 423 5 evidence evidence NN cord-022235-6ircruag 423 6 that that IN cord-022235-6ircruag 423 7 microfilaments microfilament NNS cord-022235-6ircruag 423 8 are be VBP cord-022235-6ircruag 423 9 required require VBN cord-022235-6ircruag 423 10 for for IN cord-022235-6ircruag 423 11 default default NN cord-022235-6ircruag 423 12 sorting sorting NN cord-022235-6ircruag 423 13 of of IN cord-022235-6ircruag 423 14 secretory secretory JJ cord-022235-6ircruag 423 15 proteins protein NNS cord-022235-6ircruag 423 16 ( ( -LRB- cord-022235-6ircruag 423 17 1136 1136 CD cord-022235-6ircruag 423 18 ) ) -RRB- cord-022235-6ircruag 423 19 , , , cord-022235-6ircruag 423 20 Rindler Rindler NNP cord-022235-6ircruag 423 21 et et NNP cord-022235-6ircruag 423 22 al al NNP cord-022235-6ircruag 423 23 . . . cord-022235-6ircruag 424 1 ( ( -LRB- cord-022235-6ircruag 424 2 930 930 CD cord-022235-6ircruag 424 3 ) ) -RRB- cord-022235-6ircruag 424 4 have have VBP cord-022235-6ircruag 424 5 reported report VBN cord-022235-6ircruag 424 6 that that IN cord-022235-6ircruag 424 7 colchicine colchicine NNP cord-022235-6ircruag 424 8 and and CC cord-022235-6ircruag 424 9 other other JJ cord-022235-6ircruag 424 10 microtubule microtubule NN cord-022235-6ircruag 424 11 - - HYPH cord-022235-6ircruag 424 12 disorganizing disorganize VBG cord-022235-6ircruag 424 13 agents agent NNS cord-022235-6ircruag 424 14 abolished abolish VBD cord-022235-6ircruag 424 15 specific specific JJ cord-022235-6ircruag 424 16 apical apical JJ cord-022235-6ircruag 424 17 sorting sorting NN cord-022235-6ircruag 424 18 of of IN cord-022235-6ircruag 424 19 HA HA NNP cord-022235-6ircruag 424 20 and and CC cord-022235-6ircruag 424 21 caused cause VBD cord-022235-6ircruag 424 22 influenza influenza NN cord-022235-6ircruag 424 23 virus virus NN cord-022235-6ircruag 424 24 to to TO cord-022235-6ircruag 424 25 bud bud NN cord-022235-6ircruag 424 26 from from IN cord-022235-6ircruag 424 27 both both DT cord-022235-6ircruag 424 28 cell cell NN cord-022235-6ircruag 424 29 surfaces surface NNS cord-022235-6ircruag 424 30 in in IN cord-022235-6ircruag 424 31 polarized polarized JJ cord-022235-6ircruag 424 32 cells cell NNS cord-022235-6ircruag 424 33 . . . cord-022235-6ircruag 425 1 Salas Salas NNP cord-022235-6ircruag 425 2 et et NNP cord-022235-6ircruag 425 3 al al NNP cord-022235-6ircruag 425 4 . . . cord-022235-6ircruag 426 1 ( ( -LRB- cord-022235-6ircruag 426 2 986 986 CD cord-022235-6ircruag 426 3 ) ) -RRB- cord-022235-6ircruag 426 4 obtained obtain VBD cord-022235-6ircruag 426 5 the the DT cord-022235-6ircruag 426 6 opposite opposite JJ cord-022235-6ircruag 426 7 result result NN cord-022235-6ircruag 426 8 , , , cord-022235-6ircruag 426 9 however however RB cord-022235-6ircruag 426 10 , , , cord-022235-6ircruag 426 11 and and CC cord-022235-6ircruag 426 12 neither neither DT cord-022235-6ircruag 426 13 group group NN cord-022235-6ircruag 426 14 observed observe VBD cord-022235-6ircruag 426 15 any any DT cord-022235-6ircruag 426 16 effect effect NN cord-022235-6ircruag 426 17 on on IN cord-022235-6ircruag 426 18 basolateral basolateral JJ cord-022235-6ircruag 426 19 targeting targeting NN cord-022235-6ircruag 426 20 of of IN cord-022235-6ircruag 426 21 VSV VSV NNP cord-022235-6ircruag 426 22 G G NNP cord-022235-6ircruag 426 23 protein protein NN cord-022235-6ircruag 426 24 . . . cord-022235-6ircruag 427 1 Rogalski Rogalski NNP cord-022235-6ircruag 427 2 ( ( -LRB- cord-022235-6ircruag 427 3 944 944 CD cord-022235-6ircruag 427 4 ) ) -RRB- cord-022235-6ircruag 427 5 , , , cord-022235-6ircruag 427 6 however however RB cord-022235-6ircruag 427 7 , , , cord-022235-6ircruag 427 8 found find VBD cord-022235-6ircruag 427 9 that that IN cord-022235-6ircruag 427 10 agents agent NNS cord-022235-6ircruag 427 11 which which WDT cord-022235-6ircruag 427 12 caused cause VBD cord-022235-6ircruag 427 13 microtubule microtubule NN cord-022235-6ircruag 427 14 disassembly disassembly NN cord-022235-6ircruag 427 15 caused cause VBD cord-022235-6ircruag 427 16 random random JJ cord-022235-6ircruag 427 17 sorting sorting NN cord-022235-6ircruag 427 18 of of IN cord-022235-6ircruag 427 19 G g NN cord-022235-6ircruag 427 20 protein protein NN cord-022235-6ircruag 427 21 . . . cord-022235-6ircruag 428 1 Thus thus RB cord-022235-6ircruag 428 2 , , , cord-022235-6ircruag 428 3 the the DT cord-022235-6ircruag 428 4 role role NN cord-022235-6ircruag 428 5 of of IN cord-022235-6ircruag 428 6 the the DT cord-022235-6ircruag 428 7 cytoskeleton cytoskeleton NN cord-022235-6ircruag 428 8 in in IN cord-022235-6ircruag 428 9 the the DT cord-022235-6ircruag 428 10 targeting targeting NN cord-022235-6ircruag 428 11 of of IN cord-022235-6ircruag 428 12 polarity polarity NN cord-022235-6ircruag 428 13 - - HYPH cord-022235-6ircruag 428 14 sorted sort VBN cord-022235-6ircruag 428 15 secretory secretory JJ cord-022235-6ircruag 428 16 proteins protein NNS cord-022235-6ircruag 428 17 in in IN cord-022235-6ircruag 428 18 complex complex JJ cord-022235-6ircruag 428 19 eukaryotes eukaryote NNS cord-022235-6ircruag 428 20 ' ' POS cord-022235-6ircruag 428 21 proteins protein NNS cord-022235-6ircruag 428 22 remains remain VBZ cord-022235-6ircruag 428 23 unclear unclear JJ cord-022235-6ircruag 428 24 . . . cord-022235-6ircruag 429 1 A a DT cord-022235-6ircruag 429 2 temperature temperature NN cord-022235-6ircruag 429 3 - - HYPH cord-022235-6ircruag 429 4 sensitive sensitive JJ cord-022235-6ircruag 429 5 mutation mutation NN cord-022235-6ircruag 429 6 in in IN cord-022235-6ircruag 429 7 the the DT cord-022235-6ircruag 429 8 S. S. NNP cord-022235-6ircruag 429 9 cerevisiae cerevisiae NN cord-022235-6ircruag 429 10 actin actin NN cord-022235-6ircruag 429 11 gene gene NN cord-022235-6ircruag 429 12 results result NNS cord-022235-6ircruag 429 13 in in IN cord-022235-6ircruag 429 14 abnormal abnormal JJ cord-022235-6ircruag 429 15 exocytosis exocytosis NN cord-022235-6ircruag 429 16 and and CC cord-022235-6ircruag 429 17 bud bud NN cord-022235-6ircruag 429 18 formation formation NN cord-022235-6ircruag 429 19 at at IN cord-022235-6ircruag 429 20 the the DT cord-022235-6ircruag 429 21 nonpermissive nonpermissive JJ cord-022235-6ircruag 429 22 temperature temperature NN cord-022235-6ircruag 429 23 , , , cord-022235-6ircruag 429 24 suggesting suggest VBG cord-022235-6ircruag 429 25 that that IN cord-022235-6ircruag 429 26 actin actin NN cord-022235-6ircruag 429 27 filaments filament NNS cord-022235-6ircruag 429 28 may may MD cord-022235-6ircruag 429 29 direct direct VB cord-022235-6ircruag 429 30 secretory secretory JJ cord-022235-6ircruag 429 31 vesicles vesicle NNS cord-022235-6ircruag 429 32 to to IN cord-022235-6ircruag 429 33 the the DT cord-022235-6ircruag 429 34 growing grow VBG cord-022235-6ircruag 429 35 bud bud NN cord-022235-6ircruag 429 36 in in IN cord-022235-6ircruag 429 37 yeast yeast NN cord-022235-6ircruag 429 38 cells cell NNS cord-022235-6ircruag 429 39 ( ( -LRB- cord-022235-6ircruag 429 40 793 793 CD cord-022235-6ircruag 429 41 ) ) -RRB- cord-022235-6ircruag 429 42 . . . cord-022235-6ircruag 430 1 There there EX cord-022235-6ircruag 430 2 is be VBZ cord-022235-6ircruag 430 3 evidence evidence NN cord-022235-6ircruag 430 4 to to TO cord-022235-6ircruag 430 5 show show VB cord-022235-6ircruag 430 6 that that IN cord-022235-6ircruag 430 7 the the DT cord-022235-6ircruag 430 8 cytoskeleton cytoskeleton NN cord-022235-6ircruag 430 9 may may MD cord-022235-6ircruag 430 10 be be VB cord-022235-6ircruag 430 11 important important JJ cord-022235-6ircruag 430 12 in in IN cord-022235-6ircruag 430 13 maintaining maintain VBG cord-022235-6ircruag 430 14 polarized polarized JJ cord-022235-6ircruag 430 15 distribution distribution NN cord-022235-6ircruag 430 16 in in IN cord-022235-6ircruag 430 17 complex complex JJ cord-022235-6ircruag 430 18 eukaryotic eukaryotic JJ cord-022235-6ircruag 430 19 cells cell NNS cord-022235-6ircruag 430 20 ( ( -LRB- cord-022235-6ircruag 430 21 410 410 CD cord-022235-6ircruag 430 22 ) ) -RRB- cord-022235-6ircruag 430 23 . . . cord-022235-6ircruag 431 1 A a DT cord-022235-6ircruag 431 2 polarized polarized JJ cord-022235-6ircruag 431 3 ATPase ATPase NNP cord-022235-6ircruag 431 4 has have VBZ cord-022235-6ircruag 431 5 recently recently RB cord-022235-6ircruag 431 6 been be VBN cord-022235-6ircruag 431 7 shown show VBN cord-022235-6ircruag 431 8 to to TO cord-022235-6ircruag 431 9 bind bind VB cord-022235-6ircruag 431 10 directly directly RB cord-022235-6ircruag 431 11 to to IN cord-022235-6ircruag 431 12 ankyrin ankyrin NNP cord-022235-6ircruag 431 13 , , , cord-022235-6ircruag 431 14 a a DT cord-022235-6ircruag 431 15 protein protein NN cord-022235-6ircruag 431 16 which which WDT cord-022235-6ircruag 431 17 is be VBZ cord-022235-6ircruag 431 18 known know VBN cord-022235-6ircruag 431 19 to to TO cord-022235-6ircruag 431 20 link link VB cord-022235-6ircruag 431 21 an an DT cord-022235-6ircruag 431 22 integral integral JJ cord-022235-6ircruag 431 23 erythrocyte erythrocyte NN cord-022235-6ircruag 431 24 membrane membrane NN cord-022235-6ircruag 431 25 protein protein NN cord-022235-6ircruag 431 26 to to IN cord-022235-6ircruag 431 27 spectrin spectrin NNS cord-022235-6ircruag 431 28 and and CC cord-022235-6ircruag 431 29 actin actin NN cord-022235-6ircruag 431 30 of of IN cord-022235-6ircruag 431 31 the the DT cord-022235-6ircruag 431 32 erythrocyte erythrocyte NN cord-022235-6ircruag 431 33 cytoskeleton cytoskeleton NN cord-022235-6ircruag 431 34 ( ( -LRB- cord-022235-6ircruag 431 35 770 770 CD cord-022235-6ircruag 431 36 ) ) -RRB- cord-022235-6ircruag 431 37 . . . cord-022235-6ircruag 432 1 Thus thus RB cord-022235-6ircruag 432 2 , , , cord-022235-6ircruag 432 3 ankyrin ankyrin NNP cord-022235-6ircruag 432 4 might may MD cord-022235-6ircruag 432 5 link link VB cord-022235-6ircruag 432 6 the the DT cord-022235-6ircruag 432 7 ATPase ATPase NNP cord-022235-6ircruag 432 8 to to IN cord-022235-6ircruag 432 9 microfilaments microfilament NNS cord-022235-6ircruag 432 10 and and CC cord-022235-6ircruag 432 11 thereby thereby RB cord-022235-6ircruag 432 12 maintain maintain VB cord-022235-6ircruag 432 13 its -PRON- PRP$ cord-022235-6ircruag 432 14 polar polar JJ cord-022235-6ircruag 432 15 ized ized NN cord-022235-6ircruag 432 16 distribution distribution NN cord-022235-6ircruag 432 17 . . . cord-022235-6ircruag 433 1 Two two CD cord-022235-6ircruag 433 2 features feature NNS cord-022235-6ircruag 433 3 distinguish distinguish VBP cord-022235-6ircruag 433 4 regulated regulate VBN cord-022235-6ircruag 433 5 exocytosis exocytosis NN cord-022235-6ircruag 433 6 from from IN cord-022235-6ircruag 433 7 other other JJ cord-022235-6ircruag 433 8 branches branch NNS cord-022235-6ircruag 433 9 of of IN cord-022235-6ircruag 433 10 the the DT cord-022235-6ircruag 433 11 secretory secretory JJ cord-022235-6ircruag 433 12 pathway pathway NN cord-022235-6ircruag 433 13 : : : cord-022235-6ircruag 433 14 ( ( -LRB- cord-022235-6ircruag 433 15 i i NN cord-022235-6ircruag 433 16 ) ) -RRB- cord-022235-6ircruag 434 1 Protein protein NN cord-022235-6ircruag 434 2 release release NN cord-022235-6ircruag 434 3 only only RB cord-022235-6ircruag 434 4 occurs occur VBZ cord-022235-6ircruag 434 5 when when WRB cord-022235-6ircruag 434 6 the the DT cord-022235-6ircruag 434 7 cells cell NNS cord-022235-6ircruag 434 8 are be VBP cord-022235-6ircruag 434 9 stimulated stimulate VBN cord-022235-6ircruag 434 10 by by IN cord-022235-6ircruag 434 11 secretagogues secretagogue NNS cord-022235-6ircruag 434 12 ( ( -LRB- cord-022235-6ircruag 434 13 e.g. e.g. RB cord-022235-6ircruag 434 14 , , , cord-022235-6ircruag 434 15 cyclic cyclic JJ cord-022235-6ircruag 434 16 AMP amp NN cord-022235-6ircruag 434 17 or or CC cord-022235-6ircruag 434 18 Ca Ca NNP cord-022235-6ircruag 434 19 2 2 CD cord-022235-6ircruag 434 20 + + CD cord-022235-6ircruag 434 21 ) ) -RRB- cord-022235-6ircruag 434 22 . . . cord-022235-6ircruag 435 1 ( ( -LRB- cord-022235-6ircruag 435 2 ii ii LS cord-022235-6ircruag 435 3 ) ) -RRB- cord-022235-6ircruag 435 4 Proteins protein NNS cord-022235-6ircruag 435 5 accumulate accumulate VBP cord-022235-6ircruag 435 6 within within IN cord-022235-6ircruag 435 7 the the DT cord-022235-6ircruag 435 8 cell cell NN cord-022235-6ircruag 435 9 before before IN cord-022235-6ircruag 435 10 their -PRON- PRP$ cord-022235-6ircruag 435 11 release release NN cord-022235-6ircruag 435 12 . . . cord-022235-6ircruag 436 1 Proteins protein NNS cord-022235-6ircruag 436 2 accumulate accumulate VBP cord-022235-6ircruag 436 3 in in IN cord-022235-6ircruag 436 4 a a DT cord-022235-6ircruag 436 5 special special JJ cord-022235-6ircruag 436 6 class class NN cord-022235-6ircruag 436 7 of of IN cord-022235-6ircruag 436 8 secretory secretory JJ cord-022235-6ircruag 436 9 vesicles vesicle NNS cord-022235-6ircruag 436 10 called call VBN cord-022235-6ircruag 436 11 secre secre NNP cord-022235-6ircruag 436 12 tory tory NNP cord-022235-6ircruag 436 13 granules granules NNP cord-022235-6ircruag 436 14 , , , cord-022235-6ircruag 436 15 wherein wherein WRB cord-022235-6ircruag 436 16 protein protein NN cord-022235-6ircruag 436 17 concentrations concentration NNS cord-022235-6ircruag 436 18 reach reach VBP cord-022235-6ircruag 436 19 such such JJ cord-022235-6ircruag 436 20 high high JJ cord-022235-6ircruag 436 21 levels level NNS cord-022235-6ircruag 436 22 that that WDT cord-022235-6ircruag 436 23 they -PRON- PRP cord-022235-6ircruag 436 24 become become VBP cord-022235-6ircruag 436 25 electron electron NN cord-022235-6ircruag 436 26 - - HYPH cord-022235-6ircruag 436 27 dense dense JJ cord-022235-6ircruag 436 28 and and CC cord-022235-6ircruag 436 29 can can MD cord-022235-6ircruag 436 30 be be VB cord-022235-6ircruag 436 31 clearly clearly RB cord-022235-6ircruag 436 32 identified identify VBN cord-022235-6ircruag 436 33 by by IN cord-022235-6ircruag 436 34 electron electron NNP cord-022235-6ircruag 436 35 mi mi NNP cord-022235-6ircruag 436 36 croscopy croscopy NNP cord-022235-6ircruag 436 37 . . . cord-022235-6ircruag 437 1 The the DT cord-022235-6ircruag 437 2 secretory secretory JJ cord-022235-6ircruag 437 3 pathway pathway NN cord-022235-6ircruag 437 4 is be VBZ cord-022235-6ircruag 437 5 restricted restrict VBN cord-022235-6ircruag 437 6 to to IN cord-022235-6ircruag 437 7 certain certain JJ cord-022235-6ircruag 437 8 cell cell NN cord-022235-6ircruag 437 9 types type NNS cord-022235-6ircruag 437 10 ( ( -LRB- cord-022235-6ircruag 437 11 e.g. e.g. RB cord-022235-6ircruag 437 12 , , , cord-022235-6ircruag 437 13 endocrine endocrine JJ cord-022235-6ircruag 437 14 and and CC cord-022235-6ircruag 437 15 exocrine exocrine JJ cord-022235-6ircruag 437 16 cells cell NNS cord-022235-6ircruag 437 17 , , , cord-022235-6ircruag 437 18 mast mast NN cord-022235-6ircruag 437 19 cells cell NNS cord-022235-6ircruag 437 20 ) ) -RRB- cord-022235-6ircruag 437 21 , , , cord-022235-6ircruag 437 22 and and CC cord-022235-6ircruag 437 23 only only RB cord-022235-6ircruag 437 24 certain certain JJ cord-022235-6ircruag 437 25 types type NNS cord-022235-6ircruag 437 26 of of IN cord-022235-6ircruag 437 27 pro pro JJ cord-022235-6ircruag 437 28 teins tein NNS cord-022235-6ircruag 437 29 ( ( -LRB- cord-022235-6ircruag 437 30 examples example NNS cord-022235-6ircruag 437 31 include include VBP cord-022235-6ircruag 437 32 hormones hormone NNS cord-022235-6ircruag 437 33 , , , cord-022235-6ircruag 437 34 albumin albumin NN cord-022235-6ircruag 437 35 , , , cord-022235-6ircruag 437 36 and and CC cord-022235-6ircruag 437 37 some some DT cord-022235-6ircruag 437 38 degradative degradative NNP cord-022235-6ircruag 437 39 en en NNP cord-022235-6ircruag 437 40 zymes zymes NNP cord-022235-6ircruag 437 41 including include VBG cord-022235-6ircruag 437 42 proteases protease NNS cord-022235-6ircruag 437 43 and and CC cord-022235-6ircruag 437 44 lipases lipase NNS cord-022235-6ircruag 437 45 ) ) -RRB- cord-022235-6ircruag 437 46 enter enter VB cord-022235-6ircruag 437 47 into into IN cord-022235-6ircruag 437 48 the the DT cord-022235-6ircruag 437 49 pathway pathway NN cord-022235-6ircruag 437 50 . . . cord-022235-6ircruag 438 1 Another another DT cord-022235-6ircruag 438 2 feature feature NN cord-022235-6ircruag 438 3 of of IN cord-022235-6ircruag 438 4 the the DT cord-022235-6ircruag 438 5 regulated regulate VBN cord-022235-6ircruag 438 6 secretory secretory JJ cord-022235-6ircruag 438 7 pathway pathway NN cord-022235-6ircruag 438 8 is be VBZ cord-022235-6ircruag 438 9 that that IN cord-022235-6ircruag 438 10 proteins protein NNS cord-022235-6ircruag 438 11 may may MD cord-022235-6ircruag 438 12 be be VB cord-022235-6ircruag 438 13 pro pro JJ cord-022235-6ircruag 438 14 - - JJ cord-022235-6ircruag 438 15 teolytically teolytically RB cord-022235-6ircruag 438 16 processed process VBN cord-022235-6ircruag 438 17 before before IN cord-022235-6ircruag 438 18 release release NN cord-022235-6ircruag 438 19 or or CC cord-022235-6ircruag 438 20 as as IN cord-022235-6ircruag 438 21 they -PRON- PRP cord-022235-6ircruag 438 22 are be VBP cord-022235-6ircruag 438 23 released release VBN cord-022235-6ircruag 438 24 from from IN cord-022235-6ircruag 438 25 the the DT cord-022235-6ircruag 438 26 cell cell NN cord-022235-6ircruag 438 27 , , , cord-022235-6ircruag 438 28 although although IN cord-022235-6ircruag 438 29 this this DT cord-022235-6ircruag 438 30 feature feature NN cord-022235-6ircruag 438 31 is be VBZ cord-022235-6ircruag 438 32 also also RB cord-022235-6ircruag 438 33 found find VBN cord-022235-6ircruag 438 34 in in IN cord-022235-6ircruag 438 35 some some DT cord-022235-6ircruag 438 36 constitutively constitutively RB cord-022235-6ircruag 438 37 secreted secrete VBN cord-022235-6ircruag 438 38 pro pro JJ cord-022235-6ircruag 438 39 teins tein NNS cord-022235-6ircruag 438 40 . . . cord-022235-6ircruag 439 1 Regulated regulated JJ cord-022235-6ircruag 439 2 and and CC cord-022235-6ircruag 439 3 constitutive constitutive JJ cord-022235-6ircruag 439 4 branches branch NNS cord-022235-6ircruag 439 5 of of IN cord-022235-6ircruag 439 6 the the DT cord-022235-6ircruag 439 7 secretory secretory JJ cord-022235-6ircruag 439 8 pathway pathway NN cord-022235-6ircruag 439 9 can can MD cord-022235-6ircruag 439 10 coexist coexist VB cord-022235-6ircruag 439 11 in in IN cord-022235-6ircruag 439 12 the the DT cord-022235-6ircruag 439 13 same same JJ cord-022235-6ircruag 439 14 cell cell NN cord-022235-6ircruag 439 15 ( ( -LRB- cord-022235-6ircruag 439 16 395 395 CD cord-022235-6ircruag 439 17 , , , cord-022235-6ircruag 439 18 567 567 CD cord-022235-6ircruag 439 19 , , , cord-022235-6ircruag 439 20 1109 1109 CD cord-022235-6ircruag 439 21 ) ) -RRB- cord-022235-6ircruag 439 22 , , , cord-022235-6ircruag 439 23 implying imply VBG cord-022235-6ircruag 439 24 that that IN cord-022235-6ircruag 439 25 proteins protein NNS cord-022235-6ircruag 439 26 destined destine VBN cord-022235-6ircruag 439 27 for for IN cord-022235-6ircruag 439 28 storage storage NN cord-022235-6ircruag 439 29 in in IN cord-022235-6ircruag 439 30 secretory secretory JJ cord-022235-6ircruag 439 31 granules granule NNS cord-022235-6ircruag 439 32 must must MD cord-022235-6ircruag 439 33 be be VB cord-022235-6ircruag 439 34 sorted sort VBN cord-022235-6ircruag 439 35 from from IN cord-022235-6ircruag 439 36 other other JJ cord-022235-6ircruag 439 37 secretory secretory JJ cord-022235-6ircruag 439 38 proteins protein NNS cord-022235-6ircruag 439 39 . . . cord-022235-6ircruag 440 1 Elec Elec NNP cord-022235-6ircruag 440 2 tron tron NNP cord-022235-6ircruag 440 3 microscopic microscopic NNP cord-022235-6ircruag 440 4 studies study NNS cord-022235-6ircruag 440 5 show show VBP cord-022235-6ircruag 440 6 that that IN cord-022235-6ircruag 440 7 both both DT cord-022235-6ircruag 440 8 classes class NNS cord-022235-6ircruag 440 9 of of IN cord-022235-6ircruag 440 10 secreted secrete VBN cord-022235-6ircruag 440 11 proteins protein NNS cord-022235-6ircruag 440 12 tran tran NNP cord-022235-6ircruag 440 13 sit sit VBP cord-022235-6ircruag 440 14 through through IN cord-022235-6ircruag 440 15 the the DT cord-022235-6ircruag 440 16 Golgi Golgi NNP cord-022235-6ircruag 440 17 cisternae cisternae NNP cord-022235-6ircruag 440 18 together together RB cord-022235-6ircruag 440 19 and and CC cord-022235-6ircruag 440 20 are be VBP cord-022235-6ircruag 440 21 segregated segregate VBN cord-022235-6ircruag 440 22 in in IN cord-022235-6ircruag 440 23 the the DT cord-022235-6ircruag 440 24 TGN TGN NNP cord-022235-6ircruag 440 25 , , , cord-022235-6ircruag 440 26 where where WRB cord-022235-6ircruag 440 27 proteins protein NNS cord-022235-6ircruag 440 28 destined destine VBN cord-022235-6ircruag 440 29 for for IN cord-022235-6ircruag 440 30 the the DT cord-022235-6ircruag 440 31 regulated regulated JJ cord-022235-6ircruag 440 32 branch branch NN cord-022235-6ircruag 440 33 of of IN cord-022235-6ircruag 440 34 the the DT cord-022235-6ircruag 440 35 pathway pathway NN cord-022235-6ircruag 440 36 condense condense VBP cord-022235-6ircruag 440 37 into into IN cord-022235-6ircruag 440 38 specific specific JJ cord-022235-6ircruag 440 39 areas area NNS cord-022235-6ircruag 440 40 coated coat VBN cord-022235-6ircruag 440 41 with with IN cord-022235-6ircruag 440 42 the the DT cord-022235-6ircruag 440 43 protein protein NN cord-022235-6ircruag 440 44 complex complex NN cord-022235-6ircruag 440 45 clathrin clathrin NN cord-022235-6ircruag 440 46 ( ( -LRB- cord-022235-6ircruag 440 47 see see VB cord-022235-6ircruag 440 48 Figs fig NNS cord-022235-6ircruag 440 49 . . . cord-022235-6ircruag 441 1 V.4 V.4 NNP cord-022235-6ircruag 441 2 and and CC cord-022235-6ircruag 441 3 6 6 CD cord-022235-6ircruag 441 4 ; ; : cord-022235-6ircruag 441 5 see see VB cord-022235-6ircruag 441 6 also also RB cord-022235-6ircruag 442 1 Section section NN cord-022235-6ircruag 442 2 V.G.6 v.g.6 CD cord-022235-6ircruag 442 3 ) ) -RRB- cord-022235-6ircruag 443 1 ( ( -LRB- cord-022235-6ircruag 443 2 824,1122 824,1122 CD cord-022235-6ircruag 443 3 ) ) -RRB- cord-022235-6ircruag 443 4 . . . cord-022235-6ircruag 444 1 The the DT cord-022235-6ircruag 444 2 clathrin clathrin NN cord-022235-6ircruag 444 3 coat coat NN cord-022235-6ircruag 444 4 is be VBZ cord-022235-6ircruag 444 5 subse subse NNP cord-022235-6ircruag 444 6 quently quently RB cord-022235-6ircruag 444 7 removed remove VBN cord-022235-6ircruag 444 8 as as IN cord-022235-6ircruag 444 9 the the DT cord-022235-6ircruag 444 10 condensing condense VBG cord-022235-6ircruag 444 11 granules granule NNS cord-022235-6ircruag 444 12 bud bud NN cord-022235-6ircruag 444 13 from from IN cord-022235-6ircruag 444 14 the the DT cord-022235-6ircruag 444 15 TGN TGN NNP cord-022235-6ircruag 444 16 and and CC cord-022235-6ircruag 444 17 ma ma NNP cord-022235-6ircruag 444 18 ture ture NNP cord-022235-6ircruag 444 19 ( ( -LRB- cord-022235-6ircruag 444 20 818 818 CD cord-022235-6ircruag 444 21 ) ) -RRB- cord-022235-6ircruag 444 22 ( ( -LRB- cord-022235-6ircruag 444 23 see see VB cord-022235-6ircruag 444 24 Fig Fig NNP cord-022235-6ircruag 444 25 . . . cord-022235-6ircruag 445 1 V.6 V.6 NNP cord-022235-6ircruag 445 2 ) ) -RRB- cord-022235-6ircruag 445 3 . . . cord-022235-6ircruag 446 1 These these DT cord-022235-6ircruag 446 2 areas area NNS cord-022235-6ircruag 446 3 probably probably RB cord-022235-6ircruag 446 4 represent represent VBP cord-022235-6ircruag 446 5 the the DT cord-022235-6ircruag 446 6 sites site NNS cord-022235-6ircruag 446 7 at at IN cord-022235-6ircruag 446 8 which which WDT cord-022235-6ircruag 446 9 secretory secretory NN cord-022235-6ircruag 446 10 granules granule NNS cord-022235-6ircruag 446 11 mature mature VBP cord-022235-6ircruag 446 12 and and CC cord-022235-6ircruag 446 13 are be VBP cord-022235-6ircruag 446 14 released release VBN cord-022235-6ircruag 446 15 from from IN cord-022235-6ircruag 446 16 the the DT cord-022235-6ircruag 446 17 TGN TGN NNP cord-022235-6ircruag 446 18 . . . cord-022235-6ircruag 447 1 It -PRON- PRP cord-022235-6ircruag 447 2 should should MD cord-022235-6ircruag 447 3 be be VB cord-022235-6ircruag 447 4 noted note VBN cord-022235-6ircruag 447 5 , , , cord-022235-6ircruag 447 6 however however RB cord-022235-6ircruag 447 7 , , , cord-022235-6ircruag 447 8 that that IN cord-022235-6ircruag 447 9 some some DT cord-022235-6ircruag 447 10 proteins protein NNS cord-022235-6ircruag 447 11 which which WDT cord-022235-6ircruag 447 12 are be VBP cord-022235-6ircruag 447 13 normally normally RB cord-022235-6ircruag 447 14 se se FW cord-022235-6ircruag 447 15 creted crete VBN cord-022235-6ircruag 447 16 by by IN cord-022235-6ircruag 447 17 the the DT cord-022235-6ircruag 447 18 regulated regulated JJ cord-022235-6ircruag 447 19 pathway pathway NN cord-022235-6ircruag 447 20 may may MD cord-022235-6ircruag 447 21 4 4 CD cord-022235-6ircruag 447 22 ' ' `` cord-022235-6ircruag 447 23 escape escape VB cord-022235-6ircruag 447 24 " " '' cord-022235-6ircruag 447 25 and and CC cord-022235-6ircruag 447 26 be be VB cord-022235-6ircruag 447 27 secreted secrete VBN cord-022235-6ircruag 448 1 4 4 LS cord-022235-6ircruag 448 2 ' ' `` cord-022235-6ircruag 448 3 consti consti NNP cord-022235-6ircruag 448 4 tutively tutively RB cord-022235-6ircruag 448 5 " " '' cord-022235-6ircruag 448 6 ( ( -LRB- cord-022235-6ircruag 448 7 31 31 CD cord-022235-6ircruag 448 8 ) ) -RRB- cord-022235-6ircruag 448 9 . . . cord-022235-6ircruag 449 1 It -PRON- PRP cord-022235-6ircruag 449 2 remains remain VBZ cord-022235-6ircruag 449 3 to to TO cord-022235-6ircruag 449 4 be be VB cord-022235-6ircruag 449 5 determined determine VBN cord-022235-6ircruag 449 6 whether whether IN cord-022235-6ircruag 449 7 this this DT cord-022235-6ircruag 449 8 is be VBZ cord-022235-6ircruag 449 9 due due JJ cord-022235-6ircruag 449 10 to to IN cord-022235-6ircruag 449 11 incorrect incorrect JJ cord-022235-6ircruag 449 12 sorting sorting NN cord-022235-6ircruag 449 13 or or CC cord-022235-6ircruag 449 14 to to IN cord-022235-6ircruag 449 15 low low JJ cord-022235-6ircruag 449 16 - - HYPH cord-022235-6ircruag 449 17 level level NN cord-022235-6ircruag 449 18 , , , cord-022235-6ircruag 449 19 secretagogue secretagogue NN cord-022235-6ircruag 449 20 - - HYPH cord-022235-6ircruag 449 21 independent independent JJ cord-022235-6ircruag 449 22 secretion secretion NN cord-022235-6ircruag 449 23 from from IN cord-022235-6ircruag 449 24 storage storage NN cord-022235-6ircruag 449 25 granules granule NNS cord-022235-6ircruag 449 26 , , , cord-022235-6ircruag 449 27 as as IN cord-022235-6ircruag 449 28 suggested suggest VBN cord-022235-6ircruag 449 29 by by IN cord-022235-6ircruag 449 30 studies study NNS cord-022235-6ircruag 449 31 by by IN cord-022235-6ircruag 449 32 von von NNP cord-022235-6ircruag 449 33 Zastrow Zastrow NNP cord-022235-6ircruag 449 34 and and CC cord-022235-6ircruag 449 35 Castle Castle NNP cord-022235-6ircruag 449 36 ( ( -LRB- cord-022235-6ircruag 449 37 1231 1231 CD cord-022235-6ircruag 449 38 ) ) -RRB- cord-022235-6ircruag 449 39 . . . cord-022235-6ircruag 450 1 Bur Bur NNP cord-022235-6ircruag 450 2 gess gess NNP cord-022235-6ircruag 450 3 and and CC cord-022235-6ircruag 450 4 Kelly Kelly NNP cord-022235-6ircruag 450 5 ( ( -LRB- cord-022235-6ircruag 450 6 136 136 CD cord-022235-6ircruag 450 7 ) ) -RRB- cord-022235-6ircruag 450 8 propose propose VBP cord-022235-6ircruag 450 9 that that IN cord-022235-6ircruag 450 10 this this DT cord-022235-6ircruag 450 11 " " `` cord-022235-6ircruag 450 12 spillover spillover NN cord-022235-6ircruag 450 13 " " '' cord-022235-6ircruag 450 14 secretion secretion NN cord-022235-6ircruag 450 15 may may MD cord-022235-6ircruag 450 16 be be VB cord-022235-6ircruag 450 17 due due JJ cord-022235-6ircruag 450 18 to to IN cord-022235-6ircruag 450 19 inefficient inefficient JJ cord-022235-6ircruag 450 20 sorting sorting NN cord-022235-6ircruag 450 21 in in IN cord-022235-6ircruag 450 22 the the DT cord-022235-6ircruag 450 23 specific specific JJ cord-022235-6ircruag 450 24 cell cell NN cord-022235-6ircruag 450 25 lines line NNS cord-022235-6ircruag 450 26 tested test VBN cord-022235-6ircruag 450 27 , , , cord-022235-6ircruag 450 28 since since IN cord-022235-6ircruag 450 29 Rhodes Rhodes NNP cord-022235-6ircruag 450 30 and and CC cord-022235-6ircruag 450 31 Halban Halban NNP cord-022235-6ircruag 450 32 ( ( -LRB- cord-022235-6ircruag 450 33 921 921 CD cord-022235-6ircruag 450 34 ) ) -RRB- cord-022235-6ircruag 450 35 observed observe VBD cord-022235-6ircruag 450 36 much much RB cord-022235-6ircruag 450 37 more more RBR cord-022235-6ircruag 450 38 efficient efficient JJ cord-022235-6ircruag 450 39 sorting sorting NN cord-022235-6ircruag 450 40 into into IN cord-022235-6ircruag 450 41 the the DT cord-022235-6ircruag 450 42 regulated regulated JJ cord-022235-6ircruag 450 43 pathway pathway NN cord-022235-6ircruag 450 44 . . . cord-022235-6ircruag 451 1 Efficient efficient JJ cord-022235-6ircruag 451 2 segregation segregation NN cord-022235-6ircruag 451 3 of of IN cord-022235-6ircruag 451 4 proteins protein NNS cord-022235-6ircruag 451 5 into into IN cord-022235-6ircruag 451 6 regulated regulated JJ cord-022235-6ircruag 451 7 and and CC cord-022235-6ircruag 451 8 constitutive constitutive JJ cord-022235-6ircruag 451 9 branches branch NNS cord-022235-6ircruag 451 10 of of IN cord-022235-6ircruag 451 11 the the DT cord-022235-6ircruag 451 12 secretory secretory JJ cord-022235-6ircruag 451 13 pathway pathway NN cord-022235-6ircruag 451 14 implies imply VBZ cord-022235-6ircruag 451 15 that that IN cord-022235-6ircruag 451 16 the the DT cord-022235-6ircruag 451 17 former former JJ cord-022235-6ircruag 451 18 have have VBP cord-022235-6ircruag 451 19 sorting sorting NN cord-022235-6ircruag 451 20 signals signal NNS cord-022235-6ircruag 451 21 ( ( -LRB- cord-022235-6ircruag 451 22 see see VB cord-022235-6ircruag 451 23 Section section NN cord-022235-6ircruag 451 24 V.G.4.b V.G.4.b NNP cord-022235-6ircruag 451 25 for for IN cord-022235-6ircruag 451 26 an an DT cord-022235-6ircruag 451 27 alternative alternative JJ cord-022235-6ircruag 451 28 explanation explanation NN cord-022235-6ircruag 451 29 ) ) -RRB- cord-022235-6ircruag 451 30 . . . cord-022235-6ircruag 452 1 The the DT cord-022235-6ircruag 452 2 exis exis FW cord-022235-6ircruag 452 3 tence tence NN cord-022235-6ircruag 452 4 of of IN cord-022235-6ircruag 452 5 these these DT cord-022235-6ircruag 452 6 signals signal NNS cord-022235-6ircruag 452 7 was be VBD cord-022235-6ircruag 452 8 suggested suggest VBN cord-022235-6ircruag 452 9 by by IN cord-022235-6ircruag 452 10 Moore Moore NNP cord-022235-6ircruag 452 11 and and CC cord-022235-6ircruag 452 12 Kelly Kelly NNP cord-022235-6ircruag 452 13 ( ( -LRB- cord-022235-6ircruag 452 14 731 731 CD cord-022235-6ircruag 452 15 ) ) -RRB- cord-022235-6ircruag 452 16 , , , cord-022235-6ircruag 452 17 who who WP cord-022235-6ircruag 452 18 transfected transfecte VBD cord-022235-6ircruag 452 19 a a DT cord-022235-6ircruag 452 20 pituitary pituitary JJ cord-022235-6ircruag 452 21 tumor tumor NN cord-022235-6ircruag 452 22 cell cell NN cord-022235-6ircruag 452 23 line line NN cord-022235-6ircruag 452 24 with with IN cord-022235-6ircruag 452 25 a a DT cord-022235-6ircruag 452 26 hybrid hybrid JJ cord-022235-6ircruag 452 27 gene gene NN cord-022235-6ircruag 452 28 comprising comprise VBG cord-022235-6ircruag 452 29 the the DT cord-022235-6ircruag 452 30 5 5 CD cord-022235-6ircruag 452 31 ' ' NN cord-022235-6ircruag 452 32 end end NN cord-022235-6ircruag 452 33 of of IN cord-022235-6ircruag 452 34 the the DT cord-022235-6ircruag 452 35 VSV VSV NNP cord-022235-6ircruag 452 36 G G NNP cord-022235-6ircruag 452 37 protein protein NN cord-022235-6ircruag 452 38 and and CC cord-022235-6ircruag 452 39 the the DT cord-022235-6ircruag 452 40 3 3 CD cord-022235-6ircruag 452 41 ' ' NN cord-022235-6ircruag 452 42 end end NN cord-022235-6ircruag 452 43 of of IN cord-022235-6ircruag 452 44 the the DT cord-022235-6ircruag 452 45 gene gene NN cord-022235-6ircruag 452 46 for for IN cord-022235-6ircruag 452 47 human human JJ cord-022235-6ircruag 452 48 growth growth NN cord-022235-6ircruag 452 49 hormone hormone NN cord-022235-6ircruag 452 50 . . . cord-022235-6ircruag 453 1 The the DT cord-022235-6ircruag 453 2 hybrid hybrid JJ cord-022235-6ircruag 453 3 protein protein NN cord-022235-6ircruag 453 4 was be VBD cord-022235-6ircruag 453 5 diverted divert VBN cord-022235-6ircruag 453 6 into into IN cord-022235-6ircruag 453 7 the the DT cord-022235-6ircruag 453 8 regulated regulate VBN cord-022235-6ircruag 453 9 secretory secretory JJ cord-022235-6ircruag 453 10 pathway pathway NN cord-022235-6ircruag 453 11 , , , cord-022235-6ircruag 453 12 indicating indicate VBG cord-022235-6ircruag 453 13 that that IN cord-022235-6ircruag 453 14 the the DT cord-022235-6ircruag 453 15 constitutive constitutive JJ cord-022235-6ircruag 453 16 pathway pathway NN cord-022235-6ircruag 453 17 had have VBD cord-022235-6ircruag 453 18 been be VBN cord-022235-6ircruag 453 19 bypassed bypass VBN cord-022235-6ircruag 453 20 due due IN cord-022235-6ircruag 453 21 to to IN cord-022235-6ircruag 453 22 sorting sort VBG cord-022235-6ircruag 453 23 signals signal NNS cord-022235-6ircruag 453 24 present present JJ cord-022235-6ircruag 453 25 in in IN cord-022235-6ircruag 453 26 the the DT cord-022235-6ircruag 453 27 growth growth NN cord-022235-6ircruag 453 28 hormone hormone NN cord-022235-6ircruag 453 29 part part NN cord-022235-6ircruag 453 30 of of IN cord-022235-6ircruag 453 31 the the DT cord-022235-6ircruag 453 32 hybrid hybrid NN cord-022235-6ircruag 453 33 . . . cord-022235-6ircruag 454 1 There there EX cord-022235-6ircruag 454 2 are be VBP cord-022235-6ircruag 454 3 no no DT cord-022235-6ircruag 454 4 obvious obvious JJ cord-022235-6ircruag 454 5 sequence sequence NN cord-022235-6ircruag 454 6 similarities similarity NNS cord-022235-6ircruag 454 7 between between IN cord-022235-6ircruag 454 8 proteins protein NNS cord-022235-6ircruag 454 9 secreted secrete VBN cord-022235-6ircruag 454 10 by by IN cord-022235-6ircruag 454 11 the the DT cord-022235-6ircruag 454 12 regulated regulated JJ cord-022235-6ircruag 454 13 pathway pathway NN cord-022235-6ircruag 454 14 , , , cord-022235-6ircruag 454 15 implying imply VBG cord-022235-6ircruag 454 16 that that IN cord-022235-6ircruag 454 17 the the DT cord-022235-6ircruag 454 18 sorting sorting NN cord-022235-6ircruag 454 19 signal signal NN cord-022235-6ircruag 454 20 is be VBZ cord-022235-6ircruag 454 21 probably probably RB cord-022235-6ircruag 454 22 a a DT cord-022235-6ircruag 454 23 patch patch NN cord-022235-6ircruag 454 24 signal signal NN cord-022235-6ircruag 454 25 rather rather RB cord-022235-6ircruag 454 26 than than IN cord-022235-6ircruag 454 27 any any DT cord-022235-6ircruag 454 28 identifiable identifiable JJ cord-022235-6ircruag 454 29 linear linear JJ cord-022235-6ircruag 454 30 stretch stretch NN cord-022235-6ircruag 454 31 of of IN cord-022235-6ircruag 454 32 amino amino JJ cord-022235-6ircruag 454 33 acids acid NNS cord-022235-6ircruag 454 34 . . . cord-022235-6ircruag 455 1 Several several JJ cord-022235-6ircruag 455 2 proteins protein NNS cord-022235-6ircruag 455 3 secreted secrete VBN cord-022235-6ircruag 455 4 via via IN cord-022235-6ircruag 455 5 the the DT cord-022235-6ircruag 455 6 regulated regulated JJ cord-022235-6ircruag 455 7 pathway pathway NN cord-022235-6ircruag 455 8 are be VBP cord-022235-6ircruag 455 9 proteolytically proteolytically RB cord-022235-6ircruag 455 10 processed process VBN cord-022235-6ircruag 455 11 prior prior RB cord-022235-6ircruag 455 12 to to IN cord-022235-6ircruag 455 13 their -PRON- PRP$ cord-022235-6ircruag 455 14 release release NN cord-022235-6ircruag 455 15 from from IN cord-022235-6ircruag 455 16 the the DT cord-022235-6ircruag 455 17 cell cell NN cord-022235-6ircruag 455 18 ( ( -LRB- cord-022235-6ircruag 455 19 see see VB cord-022235-6ircruag 455 20 below below RB cord-022235-6ircruag 455 21 ) ) -RRB- cord-022235-6ircruag 455 22 . . . cord-022235-6ircruag 456 1 This this DT cord-022235-6ircruag 456 2 raises raise VBZ cord-022235-6ircruag 456 3 the the DT cord-022235-6ircruag 456 4 possibility possibility NN cord-022235-6ircruag 456 5 that that IN cord-022235-6ircruag 456 6 the the DT cord-022235-6ircruag 456 7 sorting sorting NN cord-022235-6ircruag 456 8 signal signal NN cord-022235-6ircruag 456 9 could could MD cord-022235-6ircruag 456 10 reside reside VB cord-022235-6ircruag 456 11 in in IN cord-022235-6ircruag 456 12 that that DT cord-022235-6ircruag 456 13 part part NN cord-022235-6ircruag 456 14 of of IN cord-022235-6ircruag 456 15 the the DT cord-022235-6ircruag 456 16 secretory secretory JJ cord-022235-6ircruag 456 17 polypep polypep NNP cord-022235-6ircruag 456 18 tide tide NN cord-022235-6ircruag 456 19 which which WDT cord-022235-6ircruag 456 20 is be VBZ cord-022235-6ircruag 456 21 eventually eventually RB cord-022235-6ircruag 456 22 cleaved cleave VBN cord-022235-6ircruag 456 23 off off RP cord-022235-6ircruag 456 24 . . . cord-022235-6ircruag 457 1 This this DT cord-022235-6ircruag 457 2 possibility possibility NN cord-022235-6ircruag 457 3 was be VBD cord-022235-6ircruag 457 4 tested test VBN cord-022235-6ircruag 457 5 by by IN cord-022235-6ircruag 457 6 Bur Bur NNP cord-022235-6ircruag 457 7 gess gess NNP cord-022235-6ircruag 457 8 et et NNP cord-022235-6ircruag 457 9 al al NNP cord-022235-6ircruag 457 10 . . . cord-022235-6ircruag 458 1 ( ( -LRB- cord-022235-6ircruag 458 2 135 135 CD cord-022235-6ircruag 458 3 ) ) -RRB- cord-022235-6ircruag 458 4 , , , cord-022235-6ircruag 458 5 who who WP cord-022235-6ircruag 458 6 found find VBD cord-022235-6ircruag 458 7 that that IN cord-022235-6ircruag 458 8 deleting delete VBG cord-022235-6ircruag 458 9 DNA dna NN cord-022235-6ircruag 458 10 coding coding NN cord-022235-6ircruag 458 11 for for IN cord-022235-6ircruag 458 12 the the DT cord-022235-6ircruag 458 13 propeptide propeptide JJ cord-022235-6ircruag 458 14 part part NN cord-022235-6ircruag 458 15 of of IN cord-022235-6ircruag 458 16 trypsinogen trypsinogen NNP cord-022235-6ircruag 458 17 had have VBD cord-022235-6ircruag 458 18 only only RB cord-022235-6ircruag 458 19 a a DT cord-022235-6ircruag 458 20 minor minor JJ cord-022235-6ircruag 458 21 effect effect NN cord-022235-6ircruag 458 22 of of IN cord-022235-6ircruag 458 23 the the DT cord-022235-6ircruag 458 24 targeting targeting NN cord-022235-6ircruag 458 25 of of IN cord-022235-6ircruag 458 26 the the DT cord-022235-6ircruag 458 27 enzyme enzyme NN cord-022235-6ircruag 458 28 into into IN cord-022235-6ircruag 458 29 secretory secretory JJ cord-022235-6ircruag 458 30 granules granule NNS cord-022235-6ircruag 458 31 . . . cord-022235-6ircruag 459 1 Thus thus RB cord-022235-6ircruag 459 2 , , , cord-022235-6ircruag 459 3 they -PRON- PRP cord-022235-6ircruag 459 4 concluded conclude VBD cord-022235-6ircruag 459 5 , , , cord-022235-6ircruag 459 6 there there EX cord-022235-6ircruag 459 7 must must MD cord-022235-6ircruag 459 8 be be VB cord-022235-6ircruag 459 9 at at RB cord-022235-6ircruag 459 10 least least RBS cord-022235-6ircruag 459 11 one one CD cord-022235-6ircruag 459 12 sorting sorting NN cord-022235-6ircruag 459 13 signal signal NN cord-022235-6ircruag 459 14 in in IN cord-022235-6ircruag 459 15 the the DT cord-022235-6ircruag 459 16 mature mature JJ cord-022235-6ircruag 459 17 part part NN cord-022235-6ircruag 459 18 of of IN cord-022235-6ircruag 459 19 the the DT cord-022235-6ircruag 459 20 trypsinogen trypsinogen NN cord-022235-6ircruag 459 21 . . . cord-022235-6ircruag 460 1 One one CD cord-022235-6ircruag 460 2 surprising surprising JJ cord-022235-6ircruag 460 3 feature feature NN cord-022235-6ircruag 460 4 of of IN cord-022235-6ircruag 460 5 regulated regulated JJ cord-022235-6ircruag 460 6 pathway pathway NN cord-022235-6ircruag 460 7 sorting sorting NN cord-022235-6ircruag 460 8 is be VBZ cord-022235-6ircruag 460 9 that that IN cord-022235-6ircruag 460 10 the the DT cord-022235-6ircruag 460 11 putative putative JJ cord-022235-6ircruag 460 12 sorting sorting NN cord-022235-6ircruag 460 13 signal signal NN cord-022235-6ircruag 460 14 seems seem VBZ cord-022235-6ircruag 460 15 to to TO cord-022235-6ircruag 460 16 be be VB cord-022235-6ircruag 460 17 universal universal JJ cord-022235-6ircruag 460 18 . . . cord-022235-6ircruag 461 1 Moore Moore NNP cord-022235-6ircruag 461 2 et et NNP cord-022235-6ircruag 461 3 al al NNP cord-022235-6ircruag 461 4 . . . cord-022235-6ircruag 462 1 ( ( -LRB- cord-022235-6ircruag 462 2 732 732 CD cord-022235-6ircruag 462 3 ) ) -RRB- cord-022235-6ircruag 462 4 , , , cord-022235-6ircruag 462 5 for for IN cord-022235-6ircruag 462 6 example example NN cord-022235-6ircruag 462 7 , , , cord-022235-6ircruag 462 8 found find VBD cord-022235-6ircruag 462 9 that that IN cord-022235-6ircruag 462 10 human human JJ cord-022235-6ircruag 462 11 proinsulin proinsulin NN cord-022235-6ircruag 462 12 was be VBD cord-022235-6ircruag 462 13 packaged package VBN cord-022235-6ircruag 462 14 into into IN cord-022235-6ircruag 462 15 secretory secretory JJ cord-022235-6ircruag 462 16 granules granule NNS cord-022235-6ircruag 462 17 in in IN cord-022235-6ircruag 462 18 mouse mouse NN cord-022235-6ircruag 462 19 adrenocorticotropic adrenocorticotropic NNP cord-022235-6ircruag 462 20 hormone hormone NN cord-022235-6ircruag 462 21 ( ( -LRB- cord-022235-6ircruag 462 22 ACTH)-secreting ACTH)-secreting NNP cord-022235-6ircruag 462 23 cells cell NNS cord-022235-6ircruag 462 24 , , , cord-022235-6ircruag 462 25 that that IN cord-022235-6ircruag 462 26 it -PRON- PRP cord-022235-6ircruag 462 27 was be VBD cord-022235-6ircruag 462 28 correctly correctly RB cord-022235-6ircruag 462 29 processed process VBN cord-022235-6ircruag 462 30 ( ( -LRB- cord-022235-6ircruag 462 31 see see VB cord-022235-6ircruag 462 32 below below RB cord-022235-6ircruag 462 33 ) ) -RRB- cord-022235-6ircruag 462 34 , , , cord-022235-6ircruag 462 35 and and CC cord-022235-6ircruag 462 36 that that IN cord-022235-6ircruag 462 37 its -PRON- PRP$ cord-022235-6ircruag 462 38 release release NN cord-022235-6ircruag 462 39 from from IN cord-022235-6ircruag 462 40 these these DT cord-022235-6ircruag 462 41 cells cell NNS cord-022235-6ircruag 462 42 was be VBD cord-022235-6ircruag 462 43 stimulated stimulate VBN cord-022235-6ircruag 462 44 by by IN cord-022235-6ircruag 462 45 the the DT cord-022235-6ircruag 462 46 same same JJ cord-022235-6ircruag 462 47 secretagogues secretagogue NNS cord-022235-6ircruag 462 48 that that WDT cord-022235-6ircruag 462 49 stimulated stimulate VBD cord-022235-6ircruag 462 50 ACTH ACTH NNP cord-022235-6ircruag 462 51 release release NN cord-022235-6ircruag 462 52 . . . cord-022235-6ircruag 463 1 Similar similar JJ cord-022235-6ircruag 463 2 results result NNS cord-022235-6ircruag 463 3 were be VBD cord-022235-6ircruag 463 4 obtained obtain VBN cord-022235-6ircruag 463 5 in in IN cord-022235-6ircruag 463 6 studies study NNS cord-022235-6ircruag 463 7 on on IN cord-022235-6ircruag 463 8 the the DT cord-022235-6ircruag 463 9 expression expression NN cord-022235-6ircruag 463 10 of of IN cord-022235-6ircruag 463 11 human human JJ cord-022235-6ircruag 463 12 kid kid NN cord-022235-6ircruag 463 13 ney ney NNP cord-022235-6ircruag 463 14 renin renin NN cord-022235-6ircruag 463 15 DNA DNA NNP cord-022235-6ircruag 463 16 in in IN cord-022235-6ircruag 463 17 the the DT cord-022235-6ircruag 463 18 same same JJ cord-022235-6ircruag 463 19 cell cell NN cord-022235-6ircruag 463 20 line line NN cord-022235-6ircruag 463 21 ( ( -LRB- cord-022235-6ircruag 463 22 337 337 CD cord-022235-6ircruag 463 23 ) ) -RRB- cord-022235-6ircruag 463 24 . . . cord-022235-6ircruag 464 1 Fibroblast Fibroblast NNP cord-022235-6ircruag 464 2 L L NNP cord-022235-6ircruag 464 3 cells cell NNS cord-022235-6ircruag 464 4 , , , cord-022235-6ircruag 464 5 however however RB cord-022235-6ircruag 464 6 , , , cord-022235-6ircruag 464 7 which which WDT cord-022235-6ircruag 464 8 do do VBP cord-022235-6ircruag 464 9 not not RB cord-022235-6ircruag 464 10 have have VB cord-022235-6ircruag 464 11 the the DT cord-022235-6ircruag 464 12 regulated regulated JJ cord-022235-6ircruag 464 13 pathway pathway NN cord-022235-6ircruag 464 14 , , , cord-022235-6ircruag 464 15 secreted secrete VBN cord-022235-6ircruag 464 16 ( ( -LRB- cord-022235-6ircruag 464 17 unprocessed unprocessed JJ cord-022235-6ircruag 464 18 ) ) -RRB- cord-022235-6ircruag 464 19 proin proin NNP cord-022235-6ircruag 464 20 sulin sulin NNP cord-022235-6ircruag 464 21 via via IN cord-022235-6ircruag 464 22 the the DT cord-022235-6ircruag 464 23 constitutive constitutive JJ cord-022235-6ircruag 464 24 pathway pathway NN cord-022235-6ircruag 464 25 ( ( -LRB- cord-022235-6ircruag 464 26 732 732 CD cord-022235-6ircruag 464 27 ) ) -RRB- cord-022235-6ircruag 464 28 . . . cord-022235-6ircruag 465 1 Receptors receptor NNS cord-022235-6ircruag 465 2 localized localize VBN cord-022235-6ircruag 465 3 in in IN cord-022235-6ircruag 465 4 specific specific JJ cord-022235-6ircruag 465 5 domains domain NNS cord-022235-6ircruag 465 6 of of IN cord-022235-6ircruag 465 7 the the DT cord-022235-6ircruag 465 8 TGN TGN NNP cord-022235-6ircruag 465 9 membrane membrane NN cord-022235-6ircruag 465 10 may may MD cord-022235-6ircruag 465 11 segregate segregate VB cord-022235-6ircruag 465 12 proteins protein NNS cord-022235-6ircruag 465 13 into into IN cord-022235-6ircruag 465 14 the the DT cord-022235-6ircruag 465 15 regulated regulated JJ cord-022235-6ircruag 465 16 branch branch NN cord-022235-6ircruag 465 17 of of IN cord-022235-6ircruag 465 18 the the DT cord-022235-6ircruag 465 19 secretory secretory JJ cord-022235-6ircruag 465 20 pathway pathway NN cord-022235-6ircruag 465 21 , , , cord-022235-6ircruag 465 22 but but CC cord-022235-6ircruag 465 23 this this DT cord-022235-6ircruag 465 24 has have VBZ cord-022235-6ircruag 465 25 not not RB cord-022235-6ircruag 465 26 been be VBN cord-022235-6ircruag 465 27 proven prove VBN cord-022235-6ircruag 465 28 , , , cord-022235-6ircruag 465 29 and and CC cord-022235-6ircruag 465 30 other other JJ cord-022235-6ircruag 465 31 factors factor NNS cord-022235-6ircruag 465 32 may may MD cord-022235-6ircruag 465 33 be be VB cord-022235-6ircruag 465 34 important important JJ cord-022235-6ircruag 465 35 . . . cord-022235-6ircruag 466 1 For for IN cord-022235-6ircruag 466 2 exam exam NN cord-022235-6ircruag 466 3 ple ple NN cord-022235-6ircruag 466 4 , , , cord-022235-6ircruag 466 5 treatment treatment NN cord-022235-6ircruag 466 6 with with IN cord-022235-6ircruag 466 7 the the DT cord-022235-6ircruag 466 8 weak weak JJ cord-022235-6ircruag 466 9 base base NN cord-022235-6ircruag 466 10 chloroquinone chloroquinone NN cord-022235-6ircruag 466 11 causes cause VBZ cord-022235-6ircruag 466 12 ACTH ACTH NNP cord-022235-6ircruag 466 13 to to TO cord-022235-6ircruag 466 14 be be VB cord-022235-6ircruag 466 15 secreted secrete VBN cord-022235-6ircruag 466 16 by by IN cord-022235-6ircruag 466 17 the the DT cord-022235-6ircruag 466 18 constitutive constitutive JJ cord-022235-6ircruag 466 19 pathway pathway NN cord-022235-6ircruag 466 20 , , , cord-022235-6ircruag 466 21 indicating indicate VBG cord-022235-6ircruag 466 22 that that IN cord-022235-6ircruag 466 23 low low JJ cord-022235-6ircruag 466 24 pH ph NN cord-022235-6ircruag 466 25 is be VBZ cord-022235-6ircruag 466 26 required require VBN cord-022235-6ircruag 466 27 for for IN cord-022235-6ircruag 466 28 sorting sort VBG cord-022235-6ircruag 466 29 into into IN cord-022235-6ircruag 466 30 the the DT cord-022235-6ircruag 466 31 regulated regulated JJ cord-022235-6ircruag 466 32 pathway pathway NN cord-022235-6ircruag 466 33 ( ( -LRB- cord-022235-6ircruag 466 34 733 733 CD cord-022235-6ircruag 466 35 ) ) -RRB- cord-022235-6ircruag 466 36 . . . cord-022235-6ircruag 467 1 Low low JJ cord-022235-6ircruag 467 2 pH ph NN cord-022235-6ircruag 467 3 in in IN cord-022235-6ircruag 467 4 condensing condense VBG cord-022235-6ircruag 467 5 secre secre NNP cord-022235-6ircruag 467 6 tory tory NNP cord-022235-6ircruag 467 7 granules granule NNS cord-022235-6ircruag 467 8 may may MD cord-022235-6ircruag 467 9 dissociate dissociate VB cord-022235-6ircruag 467 10 proteins protein NNS cord-022235-6ircruag 467 11 from from IN cord-022235-6ircruag 467 12 their -PRON- PRP$ cord-022235-6ircruag 467 13 receptors receptor NNS cord-022235-6ircruag 467 14 , , , cord-022235-6ircruag 467 15 which which WDT cord-022235-6ircruag 467 16 can can MD cord-022235-6ircruag 467 17 then then RB cord-022235-6ircruag 467 18 be be VB cord-022235-6ircruag 467 19 reused reuse VBN cord-022235-6ircruag 467 20 ( ( -LRB- cord-022235-6ircruag 467 21 but but CC cord-022235-6ircruag 467 22 see see VB cord-022235-6ircruag 467 23 Section section NN cord-022235-6ircruag 467 24 V.G.4.b V.G.4.b NNP cord-022235-6ircruag 467 25 ) ) -RRB- cord-022235-6ircruag 467 26 . . . cord-022235-6ircruag 468 1 Another another DT cord-022235-6ircruag 468 2 feature feature NN cord-022235-6ircruag 468 3 of of IN cord-022235-6ircruag 468 4 secretory secretory JJ cord-022235-6ircruag 468 5 granules granule NNS cord-022235-6ircruag 468 6 which which WDT cord-022235-6ircruag 468 7 appears appear VBZ cord-022235-6ircruag 468 8 to to TO cord-022235-6ircruag 468 9 have have VB cord-022235-6ircruag 468 10 been be VBN cord-022235-6ircruag 468 11 largely largely RB cord-022235-6ircruag 468 12 overlooked overlooked JJ cord-022235-6ircruag 468 13 is be VBZ cord-022235-6ircruag 468 14 that that IN cord-022235-6ircruag 468 15 proteases protease NNS cord-022235-6ircruag 468 16 involved involve VBN cord-022235-6ircruag 468 17 in in IN cord-022235-6ircruag 468 18 post post JJ cord-022235-6ircruag 468 19 - - JJ cord-022235-6ircruag 468 20 TGN tgn JJ cord-022235-6ircruag 468 21 processing processing NN cord-022235-6ircruag 468 22 of of IN cord-022235-6ircruag 468 23 secretory secretory JJ cord-022235-6ircruag 468 24 proteins protein NNS cord-022235-6ircruag 468 25 must must MD cord-022235-6ircruag 468 26 also also RB cord-022235-6ircruag 468 27 carry carry VB cord-022235-6ircruag 468 28 sorting sorting NN cord-022235-6ircruag 468 29 signals signal NNS cord-022235-6ircruag 468 30 . . . cord-022235-6ircruag 469 1 It -PRON- PRP cord-022235-6ircruag 469 2 remains remain VBZ cord-022235-6ircruag 469 3 to to TO cord-022235-6ircruag 469 4 be be VB cord-022235-6ircruag 469 5 seen see VBN cord-022235-6ircruag 469 6 whether whether IN cord-022235-6ircruag 469 7 the the DT cord-022235-6ircruag 469 8 membrane membrane NN cord-022235-6ircruag 469 9 content content NN cord-022235-6ircruag 469 10 of of IN cord-022235-6ircruag 469 11 secretory secretory JJ cord-022235-6ircruag 469 12 granules granule NNS cord-022235-6ircruag 469 13 differs differ VBZ cord-022235-6ircruag 469 14 significantly significantly RB cord-022235-6ircruag 469 15 from from IN cord-022235-6ircruag 469 16 that that DT cord-022235-6ircruag 469 17 of of IN cord-022235-6ircruag 469 18 vesicles vesicle NNS cord-022235-6ircruag 469 19 of of IN cord-022235-6ircruag 469 20 the the DT cord-022235-6ircruag 469 21 consti consti NNP cord-022235-6ircruag 469 22 tutive tutive NNP cord-022235-6ircruag 469 23 branch branch NN cord-022235-6ircruag 469 24 of of IN cord-022235-6ircruag 469 25 the the DT cord-022235-6ircruag 469 26 secretory secretory JJ cord-022235-6ircruag 469 27 pathway pathway NN cord-022235-6ircruag 469 28 . . . cord-022235-6ircruag 470 1 In in IN cord-022235-6ircruag 470 2 certain certain JJ cord-022235-6ircruag 470 3 exocrine exocrine JJ cord-022235-6ircruag 470 4 cells cell NNS cord-022235-6ircruag 470 5 , , , cord-022235-6ircruag 470 6 the the DT cord-022235-6ircruag 470 7 concentration concentration NN cord-022235-6ircruag 470 8 gradient gradient NN cord-022235-6ircruag 470 9 of of IN cord-022235-6ircruag 470 10 a a DT cord-022235-6ircruag 470 11 secretory secretory JJ cord-022235-6ircruag 470 12 protein protein NN cord-022235-6ircruag 470 13 between between IN cord-022235-6ircruag 470 14 the the DT cord-022235-6ircruag 470 15 RER rer NN cord-022235-6ircruag 470 16 and and CC cord-022235-6ircruag 470 17 secretory secretory NN cord-022235-6ircruag 470 18 granules granule NNS cord-022235-6ircruag 470 19 can can MD cord-022235-6ircruag 470 20 be be VB cord-022235-6ircruag 470 21 as as RB cord-022235-6ircruag 470 22 high high JJ cord-022235-6ircruag 470 23 as as IN cord-022235-6ircruag 470 24 200 200 CD cord-022235-6ircruag 470 25 ( ( -LRB- cord-022235-6ircruag 470 26 988 988 CD cord-022235-6ircruag 470 27 ) ) -RRB- cord-022235-6ircruag 470 28 . . . cord-022235-6ircruag 471 1 The the DT cord-022235-6ircruag 471 2 dense dense JJ cord-022235-6ircruag 471 3 core core NN cord-022235-6ircruag 471 4 of of IN cord-022235-6ircruag 471 5 aggregated aggregated JJ cord-022235-6ircruag 471 6 protein protein NN cord-022235-6ircruag 471 7 is be VBZ cord-022235-6ircruag 471 8 sometimes sometimes RB cord-022235-6ircruag 471 9 seen see VBN cord-022235-6ircruag 471 10 to to TO cord-022235-6ircruag 471 11 be be VB cord-022235-6ircruag 471 12 separated separate VBN cord-022235-6ircruag 471 13 from from IN cord-022235-6ircruag 471 14 the the DT cord-022235-6ircruag 471 15 membrane membrane NN cord-022235-6ircruag 471 16 of of IN cord-022235-6ircruag 471 17 the the DT cord-022235-6ircruag 471 18 secretory secretory JJ cord-022235-6ircruag 471 19 granule granule NN cord-022235-6ircruag 471 20 ( ( -LRB- cord-022235-6ircruag 471 21 136 136 CD cord-022235-6ircruag 471 22 ) ) -RRB- cord-022235-6ircruag 471 23 and and CC cord-022235-6ircruag 471 24 may may MD cord-022235-6ircruag 471 25 remain remain VB cord-022235-6ircruag 471 26 intact intact JJ cord-022235-6ircruag 471 27 when when WRB cord-022235-6ircruag 471 28 the the DT cord-022235-6ircruag 471 29 membrane membrane NN cord-022235-6ircruag 471 30 is be VBZ cord-022235-6ircruag 471 31 removed remove VBN cord-022235-6ircruag 471 32 ( ( -LRB- cord-022235-6ircruag 471 33 1230 1230 CD cord-022235-6ircruag 471 34 ) ) -RRB- cord-022235-6ircruag 471 35 or or CC cord-022235-6ircruag 471 36 upon upon IN cord-022235-6ircruag 471 37 exocytosis exocytosis NN cord-022235-6ircruag 471 38 ( ( -LRB- cord-022235-6ircruag 471 39 19 19 CD cord-022235-6ircruag 471 40 , , , cord-022235-6ircruag 471 41 1122 1122 CD cord-022235-6ircruag 471 42 ) ) -RRB- cord-022235-6ircruag 471 43 . . . cord-022235-6ircruag 472 1 Although although IN cord-022235-6ircruag 472 2 receptor receptor NN cord-022235-6ircruag 472 3 - - HYPH cord-022235-6ircruag 472 4 mediated mediate VBN cord-022235-6ircruag 472 5 sorting sorting NN cord-022235-6ircruag 472 6 into into IN cord-022235-6ircruag 472 7 specific specific JJ cord-022235-6ircruag 472 8 regions region NNS cord-022235-6ircruag 472 9 of of IN cord-022235-6ircruag 472 10 the the DT cord-022235-6ircruag 472 11 TGN TGN NNP cord-022235-6ircruag 472 12 may may MD cord-022235-6ircruag 472 13 assist assist VB cord-022235-6ircruag 472 14 the the DT cord-022235-6ircruag 472 15 condensation condensation NN cord-022235-6ircruag 472 16 process process NN cord-022235-6ircruag 472 17 , , , cord-022235-6ircruag 472 18 proteins protein NNS cord-022235-6ircruag 472 19 secreted secrete VBN cord-022235-6ircruag 472 20 by by IN cord-022235-6ircruag 472 21 the the DT cord-022235-6ircruag 472 22 regulated regulated JJ cord-022235-6ircruag 472 23 pathway pathway NN cord-022235-6ircruag 472 24 may may MD cord-022235-6ircruag 472 25 aggregate aggregate VB cord-022235-6ircruag 472 26 spontaneously spontaneously RB cord-022235-6ircruag 472 27 and and CC cord-022235-6ircruag 472 28 be be VB cord-022235-6ircruag 472 29 packaged package VBN cord-022235-6ircruag 472 30 into into IN cord-022235-6ircruag 472 31 secretory secretory JJ cord-022235-6ircruag 472 32 granules granule NNS cord-022235-6ircruag 472 33 when when WRB cord-022235-6ircruag 472 34 they -PRON- PRP cord-022235-6ircruag 472 35 reach reach VBP cord-022235-6ircruag 472 36 a a DT cord-022235-6ircruag 472 37 critical critical JJ cord-022235-6ircruag 472 38 size size NN cord-022235-6ircruag 472 39 . . . cord-022235-6ircruag 473 1 Thus thus RB cord-022235-6ircruag 473 2 , , , cord-022235-6ircruag 473 3 the the DT cord-022235-6ircruag 473 4 packaging packaging NN cord-022235-6ircruag 473 5 of of IN cord-022235-6ircruag 473 6 G g NN cord-022235-6ircruag 473 7 protein protein NN cord-022235-6ircruag 473 8 - - HYPH cord-022235-6ircruag 473 9 growth growth NN cord-022235-6ircruag 473 10 hor hor NNP cord-022235-6ircruag 473 11 mone mone NNP cord-022235-6ircruag 473 12 hybrids hybrid NNS cord-022235-6ircruag 473 13 into into IN cord-022235-6ircruag 473 14 secretory secretory JJ cord-022235-6ircruag 473 15 granules granule NNS cord-022235-6ircruag 473 16 discussed discuss VBN cord-022235-6ircruag 473 17 above above RB cord-022235-6ircruag 473 18 ( ( -LRB- cord-022235-6ircruag 473 19 731 731 CD cord-022235-6ircruag 473 20 ) ) -RRB- cord-022235-6ircruag 473 21 may may MD cord-022235-6ircruag 473 22 be be VB cord-022235-6ircruag 473 23 due due JJ cord-022235-6ircruag 473 24 to to IN cord-022235-6ircruag 473 25 the the DT cord-022235-6ircruag 473 26 presence presence NN cord-022235-6ircruag 473 27 of of IN cord-022235-6ircruag 473 28 " " `` cord-022235-6ircruag 473 29 aggregation aggregation NN cord-022235-6ircruag 473 30 " " '' cord-022235-6ircruag 473 31 sequences sequence NNS cord-022235-6ircruag 473 32 in in IN cord-022235-6ircruag 473 33 the the DT cord-022235-6ircruag 473 34 growth growth NN cord-022235-6ircruag 473 35 hormone hormone NN cord-022235-6ircruag 473 36 segment segment NN cord-022235-6ircruag 473 37 of of IN cord-022235-6ircruag 473 38 the the DT cord-022235-6ircruag 473 39 polypeptide polypeptide NN cord-022235-6ircruag 473 40 , , , cord-022235-6ircruag 473 41 rather rather RB cord-022235-6ircruag 473 42 than than IN cord-022235-6ircruag 473 43 to to IN cord-022235-6ircruag 473 44 the the DT cord-022235-6ircruag 473 45 presence presence NN cord-022235-6ircruag 473 46 of of IN cord-022235-6ircruag 473 47 a a DT cord-022235-6ircruag 473 48 specific specific JJ cord-022235-6ircruag 473 49 sorting sorting NN cord-022235-6ircruag 473 50 signal signal NN cord-022235-6ircruag 473 51 . . . cord-022235-6ircruag 474 1 The the DT cord-022235-6ircruag 474 2 low low JJ cord-022235-6ircruag 474 3 pH ph NN cord-022235-6ircruag 474 4 of of IN cord-022235-6ircruag 474 5 the the DT cord-022235-6ircruag 474 6 condensing condensing JJ cord-022235-6ircruag 474 7 granule granule NN cord-022235-6ircruag 474 8 ( ( -LRB- cord-022235-6ircruag 474 9 19,824 19,824 CD cord-022235-6ircruag 474 10 ) ) -RRB- cord-022235-6ircruag 474 11 may may MD cord-022235-6ircruag 474 12 be be VB cord-022235-6ircruag 474 13 important important JJ cord-022235-6ircruag 474 14 for for IN cord-022235-6ircruag 474 15 protein protein NN cord-022235-6ircruag 474 16 aggregation aggregation NN cord-022235-6ircruag 474 17 , , , cord-022235-6ircruag 474 18 since since IN cord-022235-6ircruag 474 19 aggregates aggregate NNS cord-022235-6ircruag 474 20 dissociate dissociate VBP cord-022235-6ircruag 474 21 at at IN cord-022235-6ircruag 474 22 high high JJ cord-022235-6ircruag 474 23 pH ph NN cord-022235-6ircruag 474 24 ( ( -LRB- cord-022235-6ircruag 474 25 534 534 CD cord-022235-6ircruag 474 26 ) ) -RRB- cord-022235-6ircruag 474 27 . . . cord-022235-6ircruag 475 1 Pfeffer Pfeffer NNP cord-022235-6ircruag 475 2 and and CC cord-022235-6ircruag 475 3 Rothman Rothman NNP cord-022235-6ircruag 475 4 ( ( -LRB- cord-022235-6ircruag 475 5 872 872 CD cord-022235-6ircruag 475 6 ) ) -RRB- cord-022235-6ircruag 475 7 suggest suggest VBP cord-022235-6ircruag 475 8 that that IN cord-022235-6ircruag 475 9 this this DT cord-022235-6ircruag 475 10 could could MD cord-022235-6ircruag 475 11 explain explain VB cord-022235-6ircruag 475 12 the the DT cord-022235-6ircruag 475 13 failure failure NN cord-022235-6ircruag 475 14 of of IN cord-022235-6ircruag 475 15 chloroquinone chloroquinone NN cord-022235-6ircruag 475 16 - - HYPH cord-022235-6ircruag 475 17 treated treat VBN cord-022235-6ircruag 475 18 cells cell NNS cord-022235-6ircruag 475 19 to to TO cord-022235-6ircruag 475 20 package package VB cord-022235-6ircruag 475 21 secretory secretory JJ cord-022235-6ircruag 475 22 proteins protein NNS cord-022235-6ircruag 475 23 into into IN cord-022235-6ircruag 475 24 secretory secretory JJ cord-022235-6ircruag 475 25 gran gran FW cord-022235-6ircruag 475 26 ules ule NNS cord-022235-6ircruag 475 27 ( ( -LRB- cord-022235-6ircruag 475 28 see see VB cord-022235-6ircruag 475 29 above above RB cord-022235-6ircruag 475 30 ) ) -RRB- cord-022235-6ircruag 475 31 . . . cord-022235-6ircruag 476 1 Secretory secretory JJ cord-022235-6ircruag 476 2 granules granule NNS cord-022235-6ircruag 476 3 of of IN cord-022235-6ircruag 476 4 exocrine exocrine JJ cord-022235-6ircruag 476 5 ( ( -LRB- cord-022235-6ircruag 476 6 hormone hormone NN cord-022235-6ircruag 476 7 - - HYPH cord-022235-6ircruag 476 8 secreting secrete VBG cord-022235-6ircruag 476 9 ) ) -RRB- cord-022235-6ircruag 476 10 cells cell NNS cord-022235-6ircruag 476 11 remain remain VBP cord-022235-6ircruag 476 12 acidic acidic JJ cord-022235-6ircruag 476 13 during during IN cord-022235-6ircruag 476 14 maturation maturation NN cord-022235-6ircruag 476 15 , , , cord-022235-6ircruag 476 16 but but CC cord-022235-6ircruag 476 17 those those DT cord-022235-6ircruag 476 18 of of IN cord-022235-6ircruag 476 19 endocrine endocrine JJ cord-022235-6ircruag 476 20 ( ( -LRB- cord-022235-6ircruag 476 21 enzyme enzyme NN cord-022235-6ircruag 476 22 - - HYPH cord-022235-6ircruag 476 23 secret secret JJ cord-022235-6ircruag 476 24 ing ing NN cord-022235-6ircruag 476 25 ) ) -RRB- cord-022235-6ircruag 476 26 cells cell NNS cord-022235-6ircruag 476 27 return return VBP cord-022235-6ircruag 476 28 to to IN cord-022235-6ircruag 476 29 neutral neutral JJ cord-022235-6ircruag 476 30 pH ph NN cord-022235-6ircruag 476 31 as as IN cord-022235-6ircruag 476 32 they -PRON- PRP cord-022235-6ircruag 476 33 mature mature VBP cord-022235-6ircruag 476 34 ( ( -LRB- cord-022235-6ircruag 476 35 534 534 CD cord-022235-6ircruag 476 36 ) ) -RRB- cord-022235-6ircruag 476 37 . . . cord-022235-6ircruag 477 1 ATP ATP NNP cord-022235-6ircruag 477 2 is be VBZ cord-022235-6ircruag 477 3 also also RB cord-022235-6ircruag 477 4 needed need VBN cord-022235-6ircruag 477 5 for for IN cord-022235-6ircruag 477 6 secretory secretory JJ cord-022235-6ircruag 477 7 granule granule NN cord-022235-6ircruag 477 8 formation formation NN cord-022235-6ircruag 477 9 . . . cord-022235-6ircruag 478 1 There there EX cord-022235-6ircruag 478 2 are be VBP cord-022235-6ircruag 478 3 conflicting conflict VBG cord-022235-6ircruag 478 4 views view NNS cord-022235-6ircruag 478 5 as as IN cord-022235-6ircruag 478 6 to to IN cord-022235-6ircruag 478 7 whether whether IN cord-022235-6ircruag 478 8 different different JJ cord-022235-6ircruag 478 9 secretory secretory JJ cord-022235-6ircruag 478 10 proteins protein NNS cord-022235-6ircruag 478 11 cosegregate cosegregate VBP cord-022235-6ircruag 478 12 and and CC cord-022235-6ircruag 478 13 coaggregate coaggregate VBP cord-022235-6ircruag 478 14 into into IN cord-022235-6ircruag 478 15 the the DT cord-022235-6ircruag 478 16 same same JJ cord-022235-6ircruag 478 17 secretory secretory JJ cord-022235-6ircruag 478 18 granule granule NN cord-022235-6ircruag 478 19 . . . cord-022235-6ircruag 479 1 Detailed detailed JJ cord-022235-6ircruag 479 2 studies study NNS cord-022235-6ircruag 479 3 by by IN cord-022235-6ircruag 479 4 Fumagalli Fumagalli NNP cord-022235-6ircruag 479 5 and and CC cord-022235-6ircruag 479 6 Zanini Zanini NNP cord-022235-6ircruag 479 7 ( ( -LRB- cord-022235-6ircruag 479 8 341 341 CD cord-022235-6ircruag 479 9 ) ) -RRB- cord-022235-6ircruag 479 10 revealed reveal VBD cord-022235-6ircruag 479 11 that that IN cord-022235-6ircruag 479 12 bovine bovine JJ cord-022235-6ircruag 479 13 growth growth NN cord-022235-6ircruag 479 14 hor hor NNP cord-022235-6ircruag 479 15 mone mone NNP cord-022235-6ircruag 479 16 and and CC cord-022235-6ircruag 479 17 prolactin prolactin NNP cord-022235-6ircruag 479 18 could could MD cord-022235-6ircruag 479 19 be be VB cord-022235-6ircruag 479 20 present present JJ cord-022235-6ircruag 479 21 either either CC cord-022235-6ircruag 479 22 in in IN cord-022235-6ircruag 479 23 different different JJ cord-022235-6ircruag 479 24 aggregates aggregate NNS cord-022235-6ircruag 479 25 in in IN cord-022235-6ircruag 479 26 the the DT cord-022235-6ircruag 479 27 same same JJ cord-022235-6ircruag 479 28 secretory secretory JJ cord-022235-6ircruag 479 29 granule granule NN cord-022235-6ircruag 479 30 or or CC cord-022235-6ircruag 479 31 in in IN cord-022235-6ircruag 479 32 mixed mixed JJ cord-022235-6ircruag 479 33 aggregates aggregate NNS cord-022235-6ircruag 479 34 in in IN cord-022235-6ircruag 479 35 the the DT cord-022235-6ircruag 479 36 same same JJ cord-022235-6ircruag 479 37 granule granule NN cord-022235-6ircruag 479 38 or or CC cord-022235-6ircruag 479 39 in in IN cord-022235-6ircruag 479 40 pure pure JJ cord-022235-6ircruag 479 41 aggregates aggregate NNS cord-022235-6ircruag 479 42 in in IN cord-022235-6ircruag 479 43 different different JJ cord-022235-6ircruag 479 44 granules granule NNS cord-022235-6ircruag 479 45 . . . cord-022235-6ircruag 480 1 The the DT cord-022235-6ircruag 480 2 ratios ratio NNS cord-022235-6ircruag 480 3 of of IN cord-022235-6ircruag 480 4 the the DT cord-022235-6ircruag 480 5 three three CD cord-022235-6ircruag 480 6 type type NN cord-022235-6ircruag 480 7 of of IN cord-022235-6ircruag 480 8 granules granule NNS cord-022235-6ircruag 480 9 varied varied JJ cord-022235-6ircruag 480 10 from from IN cord-022235-6ircruag 480 11 animal animal NN cord-022235-6ircruag 480 12 to to IN cord-022235-6ircruag 480 13 animal animal NN cord-022235-6ircruag 480 14 . . . cord-022235-6ircruag 481 1 Similar similar JJ cord-022235-6ircruag 481 2 results result NNS cord-022235-6ircruag 481 3 were be VBD cord-022235-6ircruag 481 4 reported report VBN cord-022235-6ircruag 481 5 by by IN cord-022235-6ircruag 481 6 Mroz Mroz NNP cord-022235-6ircruag 481 7 and and CC cord-022235-6ircruag 481 8 Lechene Lechene NNP cord-022235-6ircruag 481 9 ( ( -LRB- cord-022235-6ircruag 481 10 744 744 CD cord-022235-6ircruag 481 11 ) ) -RRB- cord-022235-6ircruag 481 12 , , , cord-022235-6ircruag 481 13 who who WP cord-022235-6ircruag 481 14 showed show VBD cord-022235-6ircruag 481 15 that that IN cord-022235-6ircruag 481 16 the the DT cord-022235-6ircruag 481 17 enzyme enzyme NN cord-022235-6ircruag 481 18 content content NN cord-022235-6ircruag 481 19 of of IN cord-022235-6ircruag 481 20 individ individ JJ cord-022235-6ircruag 481 21 ual ual JJ cord-022235-6ircruag 481 22 secretory secretory JJ cord-022235-6ircruag 481 23 granules granule NNS cord-022235-6ircruag 481 24 derived derive VBN cord-022235-6ircruag 481 25 from from IN cord-022235-6ircruag 481 26 single single JJ cord-022235-6ircruag 481 27 cells cell NNS cord-022235-6ircruag 481 28 from from IN cord-022235-6ircruag 481 29 the the DT cord-022235-6ircruag 481 30 same same JJ cord-022235-6ircruag 481 31 gland gland NN cord-022235-6ircruag 481 32 can can MD cord-022235-6ircruag 481 33 vary vary VB cord-022235-6ircruag 481 34 enormously enormously RB cord-022235-6ircruag 481 35 . . . cord-022235-6ircruag 482 1 The the DT cord-022235-6ircruag 482 2 simplest simple JJS cord-022235-6ircruag 482 3 interpretation interpretation NN cord-022235-6ircruag 482 4 of of IN cord-022235-6ircruag 482 5 these these DT cord-022235-6ircruag 482 6 data datum NNS cord-022235-6ircruag 482 7 seems seem VBZ cord-022235-6ircruag 482 8 to to TO cord-022235-6ircruag 482 9 be be VB cord-022235-6ircruag 482 10 that that DT cord-022235-6ircruag 482 11 segregation segregation NN cord-022235-6ircruag 482 12 is be VBZ cord-022235-6ircruag 482 13 a a DT cord-022235-6ircruag 482 14 random random JJ cord-022235-6ircruag 482 15 process process NN cord-022235-6ircruag 482 16 and and CC cord-022235-6ircruag 482 17 that that IN cord-022235-6ircruag 482 18 the the DT cord-022235-6ircruag 482 19 formation formation NN cord-022235-6ircruag 482 20 of of IN cord-022235-6ircruag 482 21 mixed mixed JJ cord-022235-6ircruag 482 22 or or CC cord-022235-6ircruag 482 23 pure pure JJ cord-022235-6ircruag 482 24 aggregates aggregate NNS cord-022235-6ircruag 482 25 depends depend VBZ cord-022235-6ircruag 482 26 on on IN cord-022235-6ircruag 482 27 the the DT cord-022235-6ircruag 482 28 local local JJ cord-022235-6ircruag 482 29 concentration concentration NN cord-022235-6ircruag 482 30 of of IN cord-022235-6ircruag 482 31 the the DT cord-022235-6ircruag 482 32 respective respective JJ cord-022235-6ircruag 482 33 pro pro JJ cord-022235-6ircruag 482 34 teins tein NNS cord-022235-6ircruag 482 35 and and CC cord-022235-6ircruag 482 36 on on IN cord-022235-6ircruag 482 37 their -PRON- PRP$ cord-022235-6ircruag 482 38 preference preference NN cord-022235-6ircruag 482 39 for for IN cord-022235-6ircruag 482 40 forming form VBG cord-022235-6ircruag 482 41 homo homo NN cord-022235-6ircruag 482 42 - - : cord-022235-6ircruag 482 43 rather rather RB cord-022235-6ircruag 482 44 than than IN cord-022235-6ircruag 482 45 heteroaggregates heteroaggregate NNS cord-022235-6ircruag 482 46 . . . cord-022235-6ircruag 483 1 Packaging packaging NN cord-022235-6ircruag 483 2 of of IN cord-022235-6ircruag 483 3 proteins protein NNS cord-022235-6ircruag 483 4 into into IN cord-022235-6ircruag 483 5 different different JJ cord-022235-6ircruag 483 6 secretory secretory NN cord-022235-6ircruag 483 7 granules granule NNS cord-022235-6ircruag 483 8 , , , cord-022235-6ircruag 483 9 however however RB cord-022235-6ircruag 483 10 , , , cord-022235-6ircruag 483 11 might may MD cord-022235-6ircruag 483 12 permit permit VB cord-022235-6ircruag 483 13 their -PRON- PRP$ cord-022235-6ircruag 483 14 release release NN cord-022235-6ircruag 483 15 to to TO cord-022235-6ircruag 483 16 be be VB cord-022235-6ircruag 483 17 stimulated stimulate VBN cord-022235-6ircruag 483 18 by by IN cord-022235-6ircruag 483 19 different different JJ cord-022235-6ircruag 483 20 secretagogues secretagogue NNS cord-022235-6ircruag 483 21 , , , cord-022235-6ircruag 483 22 but but CC cord-022235-6ircruag 483 23 there there EX cord-022235-6ircruag 483 24 is be VBZ cord-022235-6ircruag 483 25 only only RB cord-022235-6ircruag 483 26 limited limited JJ cord-022235-6ircruag 483 27 evidence evidence NN cord-022235-6ircruag 483 28 for for IN cord-022235-6ircruag 483 29 such such PDT cord-022235-6ircruag 483 30 a a DT cord-022235-6ircruag 483 31 phenomenon phenomenon NN cord-022235-6ircruag 483 32 at at IN cord-022235-6ircruag 483 33 the the DT cord-022235-6ircruag 483 34 level level NN cord-022235-6ircruag 483 35 of of IN cord-022235-6ircruag 483 36 individual individual JJ cord-022235-6ircruag 483 37 cells cell NNS cord-022235-6ircruag 483 38 ( ( -LRB- cord-022235-6ircruag 483 39 11,136,317a 11,136,317a RB cord-022235-6ircruag 483 40 ) ) -RRB- cord-022235-6ircruag 483 41 . . . cord-022235-6ircruag 484 1 Many many JJ cord-022235-6ircruag 484 2 of of IN cord-022235-6ircruag 484 3 the the DT cord-022235-6ircruag 484 4 proteins protein NNS cord-022235-6ircruag 484 5 secreted secrete VBN cord-022235-6ircruag 484 6 by by IN cord-022235-6ircruag 484 7 the the DT cord-022235-6ircruag 484 8 regulated regulated JJ cord-022235-6ircruag 484 9 branch branch NN cord-022235-6ircruag 484 10 of of IN cord-022235-6ircruag 484 11 the the DT cord-022235-6ircruag 484 12 secretory secretory JJ cord-022235-6ircruag 484 13 pathway pathway NN cord-022235-6ircruag 484 14 are be VBP cord-022235-6ircruag 484 15 proteolytically proteolytically RB cord-022235-6ircruag 484 16 processed process VBN cord-022235-6ircruag 484 17 and and CC cord-022235-6ircruag 484 18 activated activate VBN cord-022235-6ircruag 484 19 , , , cord-022235-6ircruag 484 20 usually usually RB cord-022235-6ircruag 484 21 in in IN cord-022235-6ircruag 484 22 secretory secretory JJ cord-022235-6ircruag 484 23 granules granule NNS cord-022235-6ircruag 484 24 . . . cord-022235-6ircruag 485 1 Processing processing NN cord-022235-6ircruag 485 2 often often RB cord-022235-6ircruag 485 3 involves involve VBZ cord-022235-6ircruag 485 4 the the DT cord-022235-6ircruag 485 5 proteolytic proteolytic JJ cord-022235-6ircruag 485 6 removal removal NN cord-022235-6ircruag 485 7 of of IN cord-022235-6ircruag 485 8 an an DT cord-022235-6ircruag 485 9 N n CD cord-022235-6ircruag 485 10 - - HYPH cord-022235-6ircruag 485 11 terminal terminal NN cord-022235-6ircruag 485 12 propeptide propeptide NN cord-022235-6ircruag 485 13 and and CC cord-022235-6ircruag 485 14 can can MD cord-022235-6ircruag 485 15 be be VB cord-022235-6ircruag 485 16 mimicked mimic VBN cord-022235-6ircruag 485 17 by by IN cord-022235-6ircruag 485 18 exogenous exogenous JJ cord-022235-6ircruag 485 19 proteases protease NNS cord-022235-6ircruag 485 20 such such JJ cord-022235-6ircruag 485 21 as as IN cord-022235-6ircruag 485 22 trypsin trypsin NN cord-022235-6ircruag 485 23 ( ( -LRB- cord-022235-6ircruag 485 24 336 336 CD cord-022235-6ircruag 485 25 ) ) -RRB- cord-022235-6ircruag 485 26 . . . cord-022235-6ircruag 486 1 Alternatively alternatively RB cord-022235-6ircruag 486 2 , , , cord-022235-6ircruag 486 3 short short JJ cord-022235-6ircruag 486 4 spacer spacer NN cord-022235-6ircruag 486 5 peptides peptide NNS cord-022235-6ircruag 486 6 may may MD cord-022235-6ircruag 486 7 be be VB cord-022235-6ircruag 486 8 removed remove VBN cord-022235-6ircruag 486 9 from from IN cord-022235-6ircruag 486 10 polyprotein polyprotein JJ cord-022235-6ircruag 486 11 precursors precursor NNS cord-022235-6ircruag 486 12 ( ( -LRB- cord-022235-6ircruag 486 13 187 187 CD cord-022235-6ircruag 486 14 ) ) -RRB- cord-022235-6ircruag 486 15 . . . cord-022235-6ircruag 487 1 In in IN cord-022235-6ircruag 487 2 the the DT cord-022235-6ircruag 487 3 latter latter JJ cord-022235-6ircruag 487 4 cases case NNS cord-022235-6ircruag 487 5 , , , cord-022235-6ircruag 487 6 cells cell NNS cord-022235-6ircruag 487 7 in in IN cord-022235-6ircruag 487 8 different different JJ cord-022235-6ircruag 487 9 tissues tissue NNS cord-022235-6ircruag 487 10 can can MD cord-022235-6ircruag 487 11 process process VB cord-022235-6ircruag 487 12 the the DT cord-022235-6ircruag 487 13 precursors precursor NNS cord-022235-6ircruag 487 14 to to TO cord-022235-6ircruag 487 15 give give VB cord-022235-6ircruag 487 16 different different JJ cord-022235-6ircruag 487 17 " " `` cord-022235-6ircruag 487 18 mature mature JJ cord-022235-6ircruag 487 19 " " '' cord-022235-6ircruag 487 20 forms form NNS cord-022235-6ircruag 487 21 . . . cord-022235-6ircruag 488 1 This this DT cord-022235-6ircruag 488 2 is be VBZ cord-022235-6ircruag 488 3 the the DT cord-022235-6ircruag 488 4 case case NN cord-022235-6ircruag 488 5 for for IN cord-022235-6ircruag 488 6 prosomatostatin prosomatostatin NNP cord-022235-6ircruag 488 7 , , , cord-022235-6ircruag 488 8 which which WDT cord-022235-6ircruag 488 9 is be VBZ cord-022235-6ircruag 488 10 processed process VBN cord-022235-6ircruag 488 11 to to IN cord-022235-6ircruag 488 12 a a DT cord-022235-6ircruag 488 13 28-amino 28-amino CD cord-022235-6ircruag 488 14 - - HYPH cord-022235-6ircruag 488 15 acid acid NN cord-022235-6ircruag 488 16 form form NN cord-022235-6ircruag 488 17 by by IN cord-022235-6ircruag 488 18 cells cell NNS cord-022235-6ircruag 488 19 in in IN cord-022235-6ircruag 488 20 the the DT cord-022235-6ircruag 488 21 gut gut NN cord-022235-6ircruag 488 22 and and CC cord-022235-6ircruag 488 23 to to IN cord-022235-6ircruag 488 24 a a DT cord-022235-6ircruag 488 25 14-amino 14-amino CD cord-022235-6ircruag 488 26 - - HYPH cord-022235-6ircruag 488 27 acid acid NN cord-022235-6ircruag 488 28 form form NN cord-022235-6ircruag 488 29 by by IN cord-022235-6ircruag 488 30 brain brain NN cord-022235-6ircruag 488 31 and and CC cord-022235-6ircruag 488 32 pancreatic pancreatic NN cord-022235-6ircruag 488 33 cells cell NNS cord-022235-6ircruag 488 34 ( ( -LRB- cord-022235-6ircruag 488 35 790 790 CD cord-022235-6ircruag 488 36 ) ) -RRB- cord-022235-6ircruag 488 37 . . . cord-022235-6ircruag 489 1 Islet islet NN cord-022235-6ircruag 489 2 tissue tissue NN cord-022235-6ircruag 489 3 from from IN cord-022235-6ircruag 489 4 angler angler NN cord-022235-6ircruag 489 5 fish fish NN cord-022235-6ircruag 489 6 pancreas pancreas NN cord-022235-6ircruag 489 7 contains contain VBZ cord-022235-6ircruag 489 8 at at IN cord-022235-6ircruag 489 9 least least RBS cord-022235-6ircruag 489 10 two two CD cord-022235-6ircruag 489 11 proteases protease NNS cord-022235-6ircruag 489 12 which which WDT cord-022235-6ircruag 489 13 process process VBP cord-022235-6ircruag 489 14 prosomatostatin prosomatostatin NNP cord-022235-6ircruag 489 15 to to TO cord-022235-6ircruag 489 16 give give VB cord-022235-6ircruag 489 17 products product NNS cord-022235-6ircruag 489 18 of of IN cord-022235-6ircruag 489 19 different different JJ cord-022235-6ircruag 489 20 lengths length NNS cord-022235-6ircruag 489 21 . . . cord-022235-6ircruag 490 1 One one CD cord-022235-6ircruag 490 2 of of IN cord-022235-6ircruag 490 3 these these DT cord-022235-6ircruag 490 4 proteases protease NNS cord-022235-6ircruag 490 5 can can MD cord-022235-6ircruag 490 6 also also RB cord-022235-6ircruag 490 7 process process VB cord-022235-6ircruag 490 8 proinsulin proinsulin NNP cord-022235-6ircruag 490 9 ( ( -LRB- cord-022235-6ircruag 490 10 674 674 CD cord-022235-6ircruag 490 11 ) ) -RRB- cord-022235-6ircruag 490 12 . . . cord-022235-6ircruag 491 1 Other other JJ cord-022235-6ircruag 491 2 examples example NNS cord-022235-6ircruag 491 3 of of IN cord-022235-6ircruag 491 4 processing processing NN cord-022235-6ircruag 491 5 of of IN cord-022235-6ircruag 491 6 heterologous heterologous JJ cord-022235-6ircruag 491 7 secretory secretory JJ cord-022235-6ircruag 491 8 proteins protein NNS cord-022235-6ircruag 491 9 ( ( -LRB- cord-022235-6ircruag 491 10 188 188 CD cord-022235-6ircruag 491 11 , , , cord-022235-6ircruag 491 12 444 444 CD cord-022235-6ircruag 491 13 , , , cord-022235-6ircruag 491 14 732 732 CD cord-022235-6ircruag 491 15 ) ) -RRB- cord-022235-6ircruag 491 16 indicate indicate VBP cord-022235-6ircruag 491 17 the the DT cord-022235-6ircruag 491 18 existence existence NN cord-022235-6ircruag 491 19 of of IN cord-022235-6ircruag 491 20 only only RB cord-022235-6ircruag 491 21 a a DT cord-022235-6ircruag 491 22 limited limited JJ cord-022235-6ircruag 491 23 number number NN cord-022235-6ircruag 491 24 of of IN cord-022235-6ircruag 491 25 processing processing NN cord-022235-6ircruag 491 26 proteases protease NNS cord-022235-6ircruag 491 27 , , , cord-022235-6ircruag 491 28 which which WDT cord-022235-6ircruag 491 29 may may MD cord-022235-6ircruag 491 30 also also RB cord-022235-6ircruag 491 31 be be VB cord-022235-6ircruag 491 32 found find VBN cord-022235-6ircruag 491 33 in in IN cord-022235-6ircruag 491 34 some some DT cord-022235-6ircruag 491 35 cell cell NN cord-022235-6ircruag 491 36 types type NNS cord-022235-6ircruag 491 37 which which WDT cord-022235-6ircruag 491 38 do do VBP cord-022235-6ircruag 491 39 not not RB cord-022235-6ircruag 491 40 have have VB cord-022235-6ircruag 491 41 a a DT cord-022235-6ircruag 491 42 regulated regulate VBN cord-022235-6ircruag 491 43 secretory secretory JJ cord-022235-6ircruag 491 44 pathway pathway NN cord-022235-6ircruag 491 45 ( ( -LRB- cord-022235-6ircruag 491 46 444 444 CD cord-022235-6ircruag 491 47 , , , cord-022235-6ircruag 491 48 1168 1168 CD cord-022235-6ircruag 491 49 ) ) -RRB- cord-022235-6ircruag 491 50 . . . cord-022235-6ircruag 492 1 When when WRB cord-022235-6ircruag 492 2 secretory secretory NN cord-022235-6ircruag 492 3 proteins protein NNS cord-022235-6ircruag 492 4 which which WDT cord-022235-6ircruag 492 5 would would MD cord-022235-6ircruag 492 6 normally normally RB cord-022235-6ircruag 492 7 use use VB cord-022235-6ircruag 492 8 the the DT cord-022235-6ircruag 492 9 regulated regulated JJ cord-022235-6ircruag 492 10 pathway pathway NN cord-022235-6ircruag 492 11 are be VBP cord-022235-6ircruag 492 12 produced produce VBN cord-022235-6ircruag 492 13 in in IN cord-022235-6ircruag 492 14 cells cell NNS cord-022235-6ircruag 492 15 which which WDT cord-022235-6ircruag 492 16 do do VBP cord-022235-6ircruag 492 17 not not RB cord-022235-6ircruag 492 18 have have VB cord-022235-6ircruag 492 19 the the DT cord-022235-6ircruag 492 20 regu regu NN cord-022235-6ircruag 492 21 - - HYPH cord-022235-6ircruag 492 22 lated late VBN cord-022235-6ircruag 492 23 pathway pathway NN cord-022235-6ircruag 492 24 , , , cord-022235-6ircruag 492 25 however however RB cord-022235-6ircruag 492 26 , , , cord-022235-6ircruag 492 27 they -PRON- PRP cord-022235-6ircruag 492 28 are be VBP cord-022235-6ircruag 492 29 constitutively constitutively RB cord-022235-6ircruag 492 30 secreted secrete VBN cord-022235-6ircruag 492 31 as as IN cord-022235-6ircruag 492 32 unprocessed unprocessed JJ cord-022235-6ircruag 492 33 ( ( -LRB- cord-022235-6ircruag 492 34 pro- pro- JJ cord-022235-6ircruag 492 35 ) ) -RRB- cord-022235-6ircruag 492 36 forms form NNS cord-022235-6ircruag 492 37 ( ( -LRB- cord-022235-6ircruag 492 38 889 889 CD cord-022235-6ircruag 492 39 ) ) -RRB- cord-022235-6ircruag 492 40 . . . cord-022235-6ircruag 493 1 The the DT cord-022235-6ircruag 493 2 site site NN cord-022235-6ircruag 493 3 of of IN cord-022235-6ircruag 493 4 proinsulin proinsulin NNP cord-022235-6ircruag 493 5 processing processing NN cord-022235-6ircruag 493 6 was be VBD cord-022235-6ircruag 493 7 determined determine VBN cord-022235-6ircruag 493 8 cytochemically cytochemically RB cord-022235-6ircruag 493 9 by by IN cord-022235-6ircruag 493 10 Orci Orci NNP cord-022235-6ircruag 493 11 et et NNP cord-022235-6ircruag 493 12 al al NNP cord-022235-6ircruag 493 13 . . . cord-022235-6ircruag 494 1 ( ( -LRB- cord-022235-6ircruag 494 2 816 816 CD cord-022235-6ircruag 494 3 , , , cord-022235-6ircruag 494 4 825 825 CD cord-022235-6ircruag 494 5 ) ) -RRB- cord-022235-6ircruag 494 6 , , , cord-022235-6ircruag 494 7 using use VBG cord-022235-6ircruag 494 8 monoclonal monoclonal JJ cord-022235-6ircruag 494 9 antibodies antibody NNS cord-022235-6ircruag 494 10 specific specific JJ cord-022235-6ircruag 494 11 for for IN cord-022235-6ircruag 494 12 mature mature JJ cord-022235-6ircruag 494 13 insulin insulin NN cord-022235-6ircruag 494 14 . . . cord-022235-6ircruag 495 1 Fully fully RB cord-022235-6ircruag 495 2 processed process VBN cord-022235-6ircruag 495 3 insulin insulin NN cord-022235-6ircruag 495 4 was be VBD cord-022235-6ircruag 495 5 first first RB cord-022235-6ircruag 495 6 detected detect VBN cord-022235-6ircruag 495 7 in in IN cord-022235-6ircruag 495 8 clathrin clathrin NN cord-022235-6ircruag 495 9 - - HYPH cord-022235-6ircruag 495 10 coated coat VBN cord-022235-6ircruag 495 11 vesi vesi NNP cord-022235-6ircruag 495 12 cles cles NNP cord-022235-6ircruag 495 13 budding bud VBG cord-022235-6ircruag 495 14 from from IN cord-022235-6ircruag 495 15 the the DT cord-022235-6ircruag 495 16 TGN TGN NNP cord-022235-6ircruag 495 17 , , , cord-022235-6ircruag 495 18 and and CC cord-022235-6ircruag 495 19 subsequently subsequently RB cord-022235-6ircruag 495 20 in in IN cord-022235-6ircruag 495 21 naked naked JJ cord-022235-6ircruag 495 22 granules granule NNS cord-022235-6ircruag 495 23 . . . cord-022235-6ircruag 496 1 Process process NN cord-022235-6ircruag 496 2 ing e VBG cord-022235-6ircruag 496 3 was be VBD cord-022235-6ircruag 496 4 coincident coincident JJ cord-022235-6ircruag 496 5 with with IN cord-022235-6ircruag 496 6 condensation condensation NN cord-022235-6ircruag 496 7 and and CC cord-022235-6ircruag 496 8 acidification acidification NN cord-022235-6ircruag 496 9 and and CC cord-022235-6ircruag 496 10 was be VBD cord-022235-6ircruag 496 11 inhibited inhibit VBN cord-022235-6ircruag 496 12 at at IN cord-022235-6ircruag 496 13 higher high JJR cord-022235-6ircruag 496 14 pH pH NNP cord-022235-6ircruag 496 15 , , , cord-022235-6ircruag 496 16 indicating indicate VBG cord-022235-6ircruag 496 17 that that IN cord-022235-6ircruag 496 18 the the DT cord-022235-6ircruag 496 19 two two CD cord-022235-6ircruag 496 20 proinsulin proinsulin NN cord-022235-6ircruag 496 21 - - HYPH cord-022235-6ircruag 496 22 processing processing NN cord-022235-6ircruag 496 23 proteases protease NNS cord-022235-6ircruag 496 24 have have VBP cord-022235-6ircruag 496 25 low low JJ cord-022235-6ircruag 496 26 pH pH NNP cord-022235-6ircruag 496 27 optima optima NNP cord-022235-6ircruag 496 28 ( ( -LRB- cord-022235-6ircruag 496 29 821 821 CD cord-022235-6ircruag 496 30 ) ) -RRB- cord-022235-6ircruag 496 31 . . . cord-022235-6ircruag 497 1 Therefore therefore RB cord-022235-6ircruag 497 2 , , , cord-022235-6ircruag 497 3 this this DT cord-022235-6ircruag 497 4 and and CC cord-022235-6ircruag 497 5 other other JJ cord-022235-6ircruag 497 6 proteolytic proteolytic JJ cord-022235-6ircruag 497 7 processing processing NN cord-022235-6ircruag 497 8 steps step NNS cord-022235-6ircruag 497 9 probably probably RB cord-022235-6ircruag 497 10 occur occur VBP cord-022235-6ircruag 497 11 in in IN cord-022235-6ircruag 497 12 a a DT cord-022235-6ircruag 497 13 late late JJ cord-022235-6ircruag 497 14 " " `` cord-022235-6ircruag 497 15 compartment compartment NN cord-022235-6ircruag 497 16 " " '' cord-022235-6ircruag 497 17 of of IN cord-022235-6ircruag 497 18 the the DT cord-022235-6ircruag 497 19 TGN TGN NNP cord-022235-6ircruag 497 20 . . . cord-022235-6ircruag 498 1 Although although IN cord-022235-6ircruag 498 2 propeptides propeptide NNS cord-022235-6ircruag 498 3 may may MD cord-022235-6ircruag 498 4 not not RB cord-022235-6ircruag 498 5 play play VB cord-022235-6ircruag 498 6 a a DT cord-022235-6ircruag 498 7 role role NN cord-022235-6ircruag 498 8 in in IN cord-022235-6ircruag 498 9 protein protein NN cord-022235-6ircruag 498 10 sorting sorting NN cord-022235-6ircruag 498 11 , , , cord-022235-6ircruag 498 12 they -PRON- PRP cord-022235-6ircruag 498 13 may may MD cord-022235-6ircruag 498 14 prevent prevent VB cord-022235-6ircruag 498 15 enzymes enzyme NNS cord-022235-6ircruag 498 16 such such JJ cord-022235-6ircruag 498 17 as as IN cord-022235-6ircruag 498 18 proteases protease NNS cord-022235-6ircruag 498 19 from from IN cord-022235-6ircruag 498 20 folding fold VBG cord-022235-6ircruag 498 21 into into IN cord-022235-6ircruag 498 22 active active JJ cord-022235-6ircruag 498 23 conforma conforma NNS cord-022235-6ircruag 498 24 tions tion NNS cord-022235-6ircruag 498 25 prior prior RB cord-022235-6ircruag 498 26 to to IN cord-022235-6ircruag 498 27 their -PRON- PRP$ cord-022235-6ircruag 498 28 release release NN cord-022235-6ircruag 498 29 , , , cord-022235-6ircruag 498 30 thereby thereby RB cord-022235-6ircruag 498 31 protecting protect VBG cord-022235-6ircruag 498 32 secretory secretory JJ cord-022235-6ircruag 498 33 granules granule NNS cord-022235-6ircruag 498 34 from from IN cord-022235-6ircruag 498 35 endoproteolytic endoproteolytic JJ cord-022235-6ircruag 498 36 attack attack NN cord-022235-6ircruag 498 37 . . . cord-022235-6ircruag 499 1 Proinsulin Proinsulin NNP cord-022235-6ircruag 499 2 and and CC cord-022235-6ircruag 499 3 other other JJ cord-022235-6ircruag 499 4 prohormones prohormone NNS cord-022235-6ircruag 499 5 may may MD cord-022235-6ircruag 499 6 be be VB cord-022235-6ircruag 499 7 loosely loosely RB cord-022235-6ircruag 499 8 membrane membrane NN cord-022235-6ircruag 499 9 - - HYPH cord-022235-6ircruag 499 10 associated associate VBN cord-022235-6ircruag 499 11 ( ( -LRB- cord-022235-6ircruag 499 12 789,819 789,819 CD cord-022235-6ircruag 499 13 ) ) -RRB- cord-022235-6ircruag 499 14 . . . cord-022235-6ircruag 500 1 In in IN cord-022235-6ircruag 500 2 these these DT cord-022235-6ircruag 500 3 cases case NNS cord-022235-6ircruag 500 4 , , , cord-022235-6ircruag 500 5 bridge bridge NN cord-022235-6ircruag 500 6 sequences sequence NNS cord-022235-6ircruag 500 7 may may MD cord-022235-6ircruag 500 8 contribute contribute VB cord-022235-6ircruag 500 9 to to IN cord-022235-6ircruag 500 10 the the DT cord-022235-6ircruag 500 11 patch patch NN cord-022235-6ircruag 500 12 signal signal NN cord-022235-6ircruag 500 13 which which WDT cord-022235-6ircruag 500 14 shunts shunt VBZ cord-022235-6ircruag 500 15 proteins protein NNS cord-022235-6ircruag 500 16 into into IN cord-022235-6ircruag 500 17 budding bud VBG cord-022235-6ircruag 500 18 secre secre NNP cord-022235-6ircruag 500 19 tory tory NNP cord-022235-6ircruag 500 20 granules granule NNS cord-022235-6ircruag 500 21 . . . cord-022235-6ircruag 501 1 Relatively relatively RB cord-022235-6ircruag 501 2 little little JJ cord-022235-6ircruag 501 3 appears appear VBZ cord-022235-6ircruag 501 4 to to TO cord-022235-6ircruag 501 5 be be VB cord-022235-6ircruag 501 6 known know VBN cord-022235-6ircruag 501 7 about about IN cord-022235-6ircruag 501 8 the the DT cord-022235-6ircruag 501 9 events event NNS cord-022235-6ircruag 501 10 which which WDT cord-022235-6ircruag 501 11 accompany accompany VBP cord-022235-6ircruag 501 12 protein protein NN cord-022235-6ircruag 501 13 release release NN cord-022235-6ircruag 501 14 from from IN cord-022235-6ircruag 501 15 secretory secretory JJ cord-022235-6ircruag 501 16 granules granule NNS cord-022235-6ircruag 501 17 . . . cord-022235-6ircruag 502 1 The the DT cord-022235-6ircruag 502 2 general general JJ cord-022235-6ircruag 502 3 view view NN cord-022235-6ircruag 502 4 seems seem VBZ cord-022235-6ircruag 502 5 to to TO cord-022235-6ircruag 502 6 be be VB cord-022235-6ircruag 502 7 that that IN cord-022235-6ircruag 502 8 secretagogues secretagogue NNS cord-022235-6ircruag 502 9 directly directly RB cord-022235-6ircruag 502 10 , , , cord-022235-6ircruag 502 11 or or CC cord-022235-6ircruag 502 12 more more RBR cord-022235-6ircruag 502 13 likely likely JJ cord-022235-6ircruag 502 14 indirectly indirectly RB cord-022235-6ircruag 502 15 , , , cord-022235-6ircruag 502 16 stimulate stimulate VB cord-022235-6ircruag 502 17 fusion fusion NN cord-022235-6ircruag 502 18 between between IN cord-022235-6ircruag 502 19 the the DT cord-022235-6ircruag 502 20 granule granule NN cord-022235-6ircruag 502 21 membrane membrane NN cord-022235-6ircruag 502 22 and and CC cord-022235-6ircruag 502 23 the the DT cord-022235-6ircruag 502 24 cytoplasmic cytoplasmic JJ cord-022235-6ircruag 502 25 membrane membrane NN cord-022235-6ircruag 502 26 , , , cord-022235-6ircruag 502 27 resulting result VBG cord-022235-6ircruag 502 28 in in IN cord-022235-6ircruag 502 29 the the DT cord-022235-6ircruag 502 30 release release NN cord-022235-6ircruag 502 31 of of IN cord-022235-6ircruag 502 32 granule granule NN cord-022235-6ircruag 502 33 contents content NNS cord-022235-6ircruag 502 34 to to IN cord-022235-6ircruag 502 35 the the DT cord-022235-6ircruag 502 36 outside outside NN cord-022235-6ircruag 502 37 of of IN cord-022235-6ircruag 502 38 the the DT cord-022235-6ircruag 502 39 cell cell NN cord-022235-6ircruag 502 40 . . . cord-022235-6ircruag 503 1 Secretagogues secretagogue NNS cord-022235-6ircruag 503 2 bind bind VBP cord-022235-6ircruag 503 3 to to IN cord-022235-6ircruag 503 4 specific specific JJ cord-022235-6ircruag 503 5 cell cell NN cord-022235-6ircruag 503 6 - - HYPH cord-022235-6ircruag 503 7 surface surface NN cord-022235-6ircruag 503 8 receptors receptor NNS cord-022235-6ircruag 503 9 and and CC cord-022235-6ircruag 503 10 promote promote VB cord-022235-6ircruag 503 11 Ca Ca NNP cord-022235-6ircruag 503 12 2 2 CD cord-022235-6ircruag 503 13 + + CC cord-022235-6ircruag 503 14 influx influx NN cord-022235-6ircruag 503 15 , , , cord-022235-6ircruag 503 16 which which WDT cord-022235-6ircruag 503 17 seems seem VBZ cord-022235-6ircruag 503 18 to to TO cord-022235-6ircruag 503 19 be be VB cord-022235-6ircruag 503 20 intimately intimately RB cord-022235-6ircruag 503 21 related relate VBN cord-022235-6ircruag 503 22 to to IN cord-022235-6ircruag 503 23 the the DT cord-022235-6ircruag 503 24 fusion fusion NN cord-022235-6ircruag 503 25 event event NN cord-022235-6ircruag 503 26 ( ( -LRB- cord-022235-6ircruag 503 27 756 756 CD cord-022235-6ircruag 503 28 ) ) -RRB- cord-022235-6ircruag 503 29 . . . cord-022235-6ircruag 504 1 GTP gtp NN cord-022235-6ircruag 504 2 binding bind VBG cord-022235-6ircruag 504 3 proteins protein NNS cord-022235-6ircruag 504 4 also also RB cord-022235-6ircruag 504 5 seem seem VBP cord-022235-6ircruag 504 6 to to TO cord-022235-6ircruag 504 7 be be VB cord-022235-6ircruag 504 8 involved involve VBN cord-022235-6ircruag 504 9 in in IN cord-022235-6ircruag 504 10 generating generate VBG cord-022235-6ircruag 504 11 the the DT cord-022235-6ircruag 504 12 signal signal NN cord-022235-6ircruag 504 13 which which WDT cord-022235-6ircruag 504 14 stimu stimu NNP cord-022235-6ircruag 504 15 lates lates NNP cord-022235-6ircruag 504 16 secretion secretion NN cord-022235-6ircruag 504 17 ( ( -LRB- cord-022235-6ircruag 504 18 138 138 CD cord-022235-6ircruag 504 19 ) ) -RRB- cord-022235-6ircruag 504 20 , , , cord-022235-6ircruag 504 21 and and CC cord-022235-6ircruag 504 22 microtubules microtubule NNS cord-022235-6ircruag 504 23 may may MD cord-022235-6ircruag 504 24 play play VB cord-022235-6ircruag 504 25 a a DT cord-022235-6ircruag 504 26 minor minor JJ cord-022235-6ircruag 504 27 role role NN cord-022235-6ircruag 504 28 in in IN cord-022235-6ircruag 504 29 directing direct VBG cord-022235-6ircruag 504 30 storage storage NN cord-022235-6ircruag 504 31 granules granule NNS cord-022235-6ircruag 504 32 to to IN cord-022235-6ircruag 504 33 the the DT cord-022235-6ircruag 504 34 cell cell NN cord-022235-6ircruag 504 35 surface surface NN cord-022235-6ircruag 504 36 ( ( -LRB- cord-022235-6ircruag 504 37 112 112 CD cord-022235-6ircruag 504 38 ) ) -RRB- cord-022235-6ircruag 504 39 . . . cord-022235-6ircruag 505 1 Inhibitors Inhibitors NNP cord-022235-6ircruag 505 2 of of IN cord-022235-6ircruag 505 3 metalloprotease metalloprotease NN cord-022235-6ircruag 505 4 and and CC cord-022235-6ircruag 505 5 dipeptide dipeptide NN cord-022235-6ircruag 505 6 protease protease NN cord-022235-6ircruag 505 7 substrates substrate NNS cord-022235-6ircruag 505 8 inhibit inhibit VBP cord-022235-6ircruag 505 9 exocytosis exocytosis NN cord-022235-6ircruag 505 10 , , , cord-022235-6ircruag 505 11 suggesting suggest VBG cord-022235-6ircruag 505 12 that that IN cord-022235-6ircruag 505 13 pro pro JJ cord-022235-6ircruag 505 14 teolytic teolytic JJ cord-022235-6ircruag 505 15 cleavage cleavage NN cord-022235-6ircruag 505 16 of of IN cord-022235-6ircruag 505 17 a a DT cord-022235-6ircruag 505 18 membrane membrane NN cord-022235-6ircruag 505 19 protein protein NN cord-022235-6ircruag 505 20 may may MD cord-022235-6ircruag 505 21 be be VB cord-022235-6ircruag 505 22 essential essential JJ cord-022235-6ircruag 505 23 for for IN cord-022235-6ircruag 505 24 exocytosis exocytosis NN cord-022235-6ircruag 505 25 . . . cord-022235-6ircruag 506 1 Mundy Mundy NNP cord-022235-6ircruag 506 2 and and CC cord-022235-6ircruag 506 3 Strittmatter Strittmatter NNP cord-022235-6ircruag 506 4 ( ( -LRB- cord-022235-6ircruag 506 5 756 756 CD cord-022235-6ircruag 506 6 ) ) -RRB- cord-022235-6ircruag 506 7 , , , cord-022235-6ircruag 506 8 who who WP cord-022235-6ircruag 506 9 found find VBD cord-022235-6ircruag 506 10 that that IN cord-022235-6ircruag 506 11 metalloprotease metalloprotease NNP cord-022235-6ircruag 506 12 activity activity NN cord-022235-6ircruag 506 13 was be VBD cord-022235-6ircruag 506 14 highest high JJS cord-022235-6ircruag 506 15 in in IN cord-022235-6ircruag 506 16 the the DT cord-022235-6ircruag 506 17 plasma plasma NN cord-022235-6ircruag 506 18 membrane membrane NN cord-022235-6ircruag 506 19 , , , cord-022235-6ircruag 506 20 propose propose VBP cord-022235-6ircruag 506 21 that that IN cord-022235-6ircruag 506 22 proteolysis proteolysis NN cord-022235-6ircruag 506 23 may may MD cord-022235-6ircruag 506 24 un un NNP cord-022235-6ircruag 506 25 mask mask VB cord-022235-6ircruag 506 26 the the DT cord-022235-6ircruag 506 27 active active JJ cord-022235-6ircruag 506 28 site site NN cord-022235-6ircruag 506 29 on on IN cord-022235-6ircruag 506 30 a a DT cord-022235-6ircruag 506 31 fusogenic fusogenic JJ cord-022235-6ircruag 506 32 membrane membrane NN cord-022235-6ircruag 506 33 protein protein NN cord-022235-6ircruag 506 34 . . . cord-022235-6ircruag 507 1 Breckenbridge Breckenbridge NNP cord-022235-6ircruag 507 2 and and CC cord-022235-6ircruag 507 3 Aimers Aimers NNP cord-022235-6ircruag 507 4 ( ( -LRB- cord-022235-6ircruag 507 5 121 121 CD cord-022235-6ircruag 507 6 ) ) -RRB- cord-022235-6ircruag 507 7 have have VBP cord-022235-6ircruag 507 8 recently recently RB cord-022235-6ircruag 507 9 studied study VBN cord-022235-6ircruag 507 10 exocytosis exocytosis NN cord-022235-6ircruag 507 11 - - HYPH cord-022235-6ircruag 507 12 associated associate VBN cord-022235-6ircruag 507 13 changes change NNS cord-022235-6ircruag 507 14 in in IN cord-022235-6ircruag 507 15 membrane membrane NN cord-022235-6ircruag 507 16 capacitance capacitance NN cord-022235-6ircruag 507 17 in in IN cord-022235-6ircruag 507 18 a a DT cord-022235-6ircruag 507 19 mouse mouse NN cord-022235-6ircruag 507 20 mast mast NN cord-022235-6ircruag 507 21 cell cell NN cord-022235-6ircruag 507 22 mutant mutant JJ cord-022235-6ircruag 507 23 with with IN cord-022235-6ircruag 507 24 enlarged enlarged JJ cord-022235-6ircruag 507 25 secretory secretory NN cord-022235-6ircruag 507 26 granules granule NNS cord-022235-6ircruag 507 27 . . . cord-022235-6ircruag 508 1 Small small JJ cord-022235-6ircruag 508 2 fluctuations fluctuation NNS cord-022235-6ircruag 508 3 in in IN cord-022235-6ircruag 508 4 capacitance capacitance NN cord-022235-6ircruag 508 5 preceded precede VBD cord-022235-6ircruag 508 6 larger large JJR cord-022235-6ircruag 508 7 increases increase NNS cord-022235-6ircruag 508 8 , , , cord-022235-6ircruag 508 9 which which WDT cord-022235-6ircruag 508 10 themselves -PRON- PRP cord-022235-6ircruag 508 11 preceded precede VBD cord-022235-6ircruag 508 12 granule granule NN cord-022235-6ircruag 508 13 swelling swell VBG cord-022235-6ircruag 508 14 and and CC cord-022235-6ircruag 508 15 the the DT cord-022235-6ircruag 508 16 release release NN cord-022235-6ircruag 508 17 of of IN cord-022235-6ircruag 508 18 a a DT cord-022235-6ircruag 508 19 fluorescent fluorescent JJ cord-022235-6ircruag 508 20 tracer tracer NN cord-022235-6ircruag 508 21 dye dye NN cord-022235-6ircruag 508 22 from from IN cord-022235-6ircruag 508 23 the the DT cord-022235-6ircruag 508 24 lumen lumen NN cord-022235-6ircruag 508 25 of of IN cord-022235-6ircruag 508 26 the the DT cord-022235-6ircruag 508 27 granule granule NN cord-022235-6ircruag 508 28 . . . cord-022235-6ircruag 509 1 The the DT cord-022235-6ircruag 509 2 large large JJ cord-022235-6ircruag 509 3 increase increase NN cord-022235-6ircruag 509 4 in in IN cord-022235-6ircruag 509 5 capacitance capacitance NN cord-022235-6ircruag 509 6 probably probably RB cord-022235-6ircruag 509 7 results result VBZ cord-022235-6ircruag 509 8 from from IN cord-022235-6ircruag 509 9 productive productive JJ cord-022235-6ircruag 509 10 fusion fusion NN cord-022235-6ircruag 509 11 be be VB cord-022235-6ircruag 509 12 tween tween VBN cord-022235-6ircruag 509 13 the the DT cord-022235-6ircruag 509 14 plasma plasma NN cord-022235-6ircruag 509 15 and and CC cord-022235-6ircruag 509 16 granule granule NN cord-022235-6ircruag 509 17 membranes membrane NNS cord-022235-6ircruag 509 18 ( ( -LRB- cord-022235-6ircruag 509 19 312 312 CD cord-022235-6ircruag 509 20 ) ) -RRB- cord-022235-6ircruag 509 21 , , , cord-022235-6ircruag 509 22 whereas whereas IN cord-022235-6ircruag 509 23 capacitance capacitance NN cord-022235-6ircruag 509 24 " " `` cord-022235-6ircruag 509 25 flutters flutter NNS cord-022235-6ircruag 509 26 " " '' cord-022235-6ircruag 509 27 may may MD cord-022235-6ircruag 509 28 represent represent VB cord-022235-6ircruag 509 29 nonproductive nonproductive JJ cord-022235-6ircruag 509 30 membrane membrane NNP cord-022235-6ircruag 509 31 association association NN cord-022235-6ircruag 509 32 . . . cord-022235-6ircruag 510 1 The the DT cord-022235-6ircruag 510 2 most most RBS cord-022235-6ircruag 510 3 plausible plausible JJ cord-022235-6ircruag 510 4 interpretation interpretation NN cord-022235-6ircruag 510 5 of of IN cord-022235-6ircruag 510 6 these these DT cord-022235-6ircruag 510 7 data datum NNS cord-022235-6ircruag 510 8 is be VBZ cord-022235-6ircruag 510 9 that that DT cord-022235-6ircruag 510 10 release release NN cord-022235-6ircruag 510 11 of of IN cord-022235-6ircruag 510 12 secretory secretory JJ cord-022235-6ircruag 510 13 granule granule NN cord-022235-6ircruag 510 14 contents content NNS cord-022235-6ircruag 510 15 is be VBZ cord-022235-6ircruag 510 16 preceded precede VBN cord-022235-6ircruag 510 17 by by IN cord-022235-6ircruag 510 18 the the DT cord-022235-6ircruag 510 19 formation formation NN cord-022235-6ircruag 510 20 of of IN cord-022235-6ircruag 510 21 a a DT cord-022235-6ircruag 510 22 narrow narrow JJ cord-022235-6ircruag 510 23 channel channel NN cord-022235-6ircruag 510 24 , , , cord-022235-6ircruag 510 25 the the DT cord-022235-6ircruag 510 26 fusion fusion NN cord-022235-6ircruag 510 27 pore pore NN cord-022235-6ircruag 510 28 , , , cord-022235-6ircruag 510 29 ( ( -LRB- cord-022235-6ircruag 510 30 121 121 CD cord-022235-6ircruag 510 31 ) ) -RRB- cord-022235-6ircruag 510 32 between between IN cord-022235-6ircruag 510 33 the the DT cord-022235-6ircruag 510 34 two two CD cord-022235-6ircruag 510 35 membranes membrane NNS cord-022235-6ircruag 510 36 , , , cord-022235-6ircruag 510 37 leading lead VBG cord-022235-6ircruag 510 38 eventually eventually RB cord-022235-6ircruag 510 39 to to IN cord-022235-6ircruag 510 40 the the DT cord-022235-6ircruag 510 41 open open JJ cord-022235-6ircruag 510 42 ing ing NN cord-022235-6ircruag 510 43 of of IN cord-022235-6ircruag 510 44 the the DT cord-022235-6ircruag 510 45 granule granule NN cord-022235-6ircruag 510 46 membrane membrane NN cord-022235-6ircruag 510 47 to to IN cord-022235-6ircruag 510 48 the the DT cord-022235-6ircruag 510 49 outside outside NN cord-022235-6ircruag 510 50 of of IN cord-022235-6ircruag 510 51 the the DT cord-022235-6ircruag 510 52 cell cell NN cord-022235-6ircruag 510 53 and and CC cord-022235-6ircruag 510 54 the the DT cord-022235-6ircruag 510 55 subsequent subsequent JJ cord-022235-6ircruag 510 56 swelling swelling NN cord-022235-6ircruag 510 57 and and CC cord-022235-6ircruag 510 58 dissociation dissociation NN cord-022235-6ircruag 510 59 of of IN cord-022235-6ircruag 510 60 the the DT cord-022235-6ircruag 510 61 granule granule NN cord-022235-6ircruag 510 62 contents content NNS cord-022235-6ircruag 510 63 and and CC cord-022235-6ircruag 510 64 their -PRON- PRP$ cord-022235-6ircruag 510 65 release release NN cord-022235-6ircruag 510 66 as as IN cord-022235-6ircruag 510 67 soluble soluble JJ cord-022235-6ircruag 510 68 proteins protein NNS cord-022235-6ircruag 510 69 . . . cord-022235-6ircruag 511 1 Lysosomes Lysosomes NNP cord-022235-6ircruag 511 2 ( ( -LRB- cord-022235-6ircruag 511 3 in in IN cord-022235-6ircruag 511 4 animal animal NN cord-022235-6ircruag 511 5 cells cell NNS cord-022235-6ircruag 511 6 ) ) -RRB- cord-022235-6ircruag 511 7 and and CC cord-022235-6ircruag 511 8 vacuoles vacuole NNS cord-022235-6ircruag 511 9 ( ( -LRB- cord-022235-6ircruag 511 10 in in IN cord-022235-6ircruag 511 11 plant plant NN cord-022235-6ircruag 511 12 and and CC cord-022235-6ircruag 511 13 fungal fungal NN cord-022235-6ircruag 511 14 cells cell NNS cord-022235-6ircruag 511 15 ) ) -RRB- cord-022235-6ircruag 511 16 con con NN cord-022235-6ircruag 511 17 tain tain XX cord-022235-6ircruag 511 18 most most JJS cord-022235-6ircruag 511 19 of of IN cord-022235-6ircruag 511 20 the the DT cord-022235-6ircruag 511 21 cells cell NNS cord-022235-6ircruag 511 22 ' ' POS cord-022235-6ircruag 511 23 degradative degradative JJ cord-022235-6ircruag 511 24 enzymes enzyme NNS cord-022235-6ircruag 511 25 , , , cord-022235-6ircruag 511 26 which which WDT cord-022235-6ircruag 511 27 function function VBP cord-022235-6ircruag 511 28 not not RB cord-022235-6ircruag 511 29 only only RB cord-022235-6ircruag 511 30 in in IN cord-022235-6ircruag 511 31 general general JJ cord-022235-6ircruag 511 32 " " `` cord-022235-6ircruag 511 33 housekeeping housekeeping NN cord-022235-6ircruag 511 34 " " '' cord-022235-6ircruag 511 35 but but CC cord-022235-6ircruag 511 36 also also RB cord-022235-6ircruag 511 37 in in IN cord-022235-6ircruag 511 38 the the DT cord-022235-6ircruag 511 39 degradation degradation NN cord-022235-6ircruag 511 40 of of IN cord-022235-6ircruag 511 41 endocytosed endocytosed JJ cord-022235-6ircruag 511 42 mate mate NN cord-022235-6ircruag 511 43 rial rial NNP cord-022235-6ircruag 511 44 ( ( -LRB- cord-022235-6ircruag 511 45 Chapter chapter NN cord-022235-6ircruag 511 46 VIII VIII NNP cord-022235-6ircruag 511 47 ) ) -RRB- cord-022235-6ircruag 511 48 . . . cord-022235-6ircruag 512 1 The the DT cord-022235-6ircruag 512 2 following following JJ cord-022235-6ircruag 512 3 sections section NNS cord-022235-6ircruag 512 4 review review VBP cord-022235-6ircruag 512 5 the the DT cord-022235-6ircruag 512 6 evidence evidence NN cord-022235-6ircruag 512 7 for for IN cord-022235-6ircruag 512 8 lysoso lysoso NNP cord-022235-6ircruag 512 9 mal mal NNP cord-022235-6ircruag 512 10 and and CC cord-022235-6ircruag 512 11 vacuolar vacuolar NNP cord-022235-6ircruag 512 12 sorting sorting NN cord-022235-6ircruag 512 13 signals signal NNS cord-022235-6ircruag 512 14 , , , cord-022235-6ircruag 512 15 special special JJ cord-022235-6ircruag 512 16 features feature NNS cord-022235-6ircruag 512 17 of of IN cord-022235-6ircruag 512 18 the the DT cord-022235-6ircruag 512 19 sorting sort VBG cord-022235-6ircruag 512 20 pathways pathway NNS cord-022235-6ircruag 512 21 , , , cord-022235-6ircruag 512 22 and and CC cord-022235-6ircruag 512 23 differences difference NNS cord-022235-6ircruag 512 24 between between IN cord-022235-6ircruag 512 25 the the DT cord-022235-6ircruag 512 26 lysosomal lysosomal JJ cord-022235-6ircruag 512 27 and and CC cord-022235-6ircruag 512 28 vacuolar vacuolar JJ cord-022235-6ircruag 512 29 routes route NNS cord-022235-6ircruag 512 30 . . . cord-022235-6ircruag 513 1 Specific specific JJ cord-022235-6ircruag 513 2 sorting sorting NN cord-022235-6ircruag 513 3 of of IN cord-022235-6ircruag 513 4 soluble soluble JJ cord-022235-6ircruag 513 5 lysosomal lysosomal JJ cord-022235-6ircruag 513 6 enzymes enzyme NNS cord-022235-6ircruag 513 7 is be VBZ cord-022235-6ircruag 513 8 determined determine VBN cord-022235-6ircruag 513 9 by by IN cord-022235-6ircruag 513 10 mannose-6-phosphate mannose-6-phosphate NNP cord-022235-6ircruag 513 11 ( ( -LRB- cord-022235-6ircruag 513 12 M6P m6p NN cord-022235-6ircruag 513 13 ) ) -RRB- cord-022235-6ircruag 513 14 residues residue NNS cord-022235-6ircruag 513 15 on on IN cord-022235-6ircruag 513 16 N n CD cord-022235-6ircruag 513 17 - - HYPH cord-022235-6ircruag 513 18 linked link VBN cord-022235-6ircruag 513 19 core core NN cord-022235-6ircruag 513 20 oligosaccharides oligosaccharide NNS cord-022235-6ircruag 513 21 ( ( -LRB- cord-022235-6ircruag 513 22 Section section NN cord-022235-6ircruag 513 23 V.D.I.a V.D.I.a NNP cord-022235-6ircruag 513 24 ) ) -RRB- cord-022235-6ircruag 513 25 . . . cord-022235-6ircruag 514 1 Two two CD cord-022235-6ircruag 514 2 receptors receptor NNS cord-022235-6ircruag 514 3 have have VBP cord-022235-6ircruag 514 4 been be VBN cord-022235-6ircruag 514 5 identified identify VBN cord-022235-6ircruag 514 6 . . . cord-022235-6ircruag 515 1 The the DT cord-022235-6ircruag 515 2 major major JJ cord-022235-6ircruag 515 3 M6P m6p NN cord-022235-6ircruag 515 4 receptor receptor NN cord-022235-6ircruag 515 5 ( ( -LRB- cord-022235-6ircruag 515 6 275 275 CD cord-022235-6ircruag 515 7 kDa kDa NNS cord-022235-6ircruag 515 8 , , , cord-022235-6ircruag 515 9 formerly formerly RB cord-022235-6ircruag 515 10 called call VBD cord-022235-6ircruag 515 11 the the DT cord-022235-6ircruag 515 12 215-kDa 215-kda CD cord-022235-6ircruag 515 13 receptor receptor NN cord-022235-6ircruag 515 14 ) ) -RRB- cord-022235-6ircruag 515 15 was be VBD cord-022235-6ircruag 515 16 detected detect VBN cord-022235-6ircruag 515 17 predomi predomi NNP cord-022235-6ircruag 515 18 nantly nantly RB cord-022235-6ircruag 515 19 in in IN cord-022235-6ircruag 515 20 the the DT cord-022235-6ircruag 515 21 cis cis NNP cord-022235-6ircruag 515 22 Golgi Golgi NNP cord-022235-6ircruag 515 23 compartment compartment NN cord-022235-6ircruag 515 24 ( ( -LRB- cord-022235-6ircruag 515 25 131 131 CD cord-022235-6ircruag 515 26 ) ) -RRB- cord-022235-6ircruag 515 27 , , , cord-022235-6ircruag 515 28 leading lead VBG cord-022235-6ircruag 515 29 to to IN cord-022235-6ircruag 515 30 the the DT cord-022235-6ircruag 515 31 proposal proposal NN cord-022235-6ircruag 515 32 that that IN cord-022235-6ircruag 515 33 the the DT cord-022235-6ircruag 515 34 lysosomal lysosomal JJ cord-022235-6ircruag 515 35 pathway pathway NN cord-022235-6ircruag 515 36 diverged diverge VBD cord-022235-6ircruag 515 37 from from IN cord-022235-6ircruag 515 38 the the DT cord-022235-6ircruag 515 39 main main JJ cord-022235-6ircruag 515 40 secretory secretory JJ cord-022235-6ircruag 515 41 pathway pathway NN cord-022235-6ircruag 515 42 at at IN cord-022235-6ircruag 515 43 the the DT cord-022235-6ircruag 515 44 cis cis NNP cord-022235-6ircruag 515 45 end end NN cord-022235-6ircruag 515 46 of of IN cord-022235-6ircruag 515 47 the the DT cord-022235-6ircruag 515 48 Golgi Golgi NNP cord-022235-6ircruag 515 49 stack stack NN cord-022235-6ircruag 515 50 rather rather RB cord-022235-6ircruag 515 51 than than IN cord-022235-6ircruag 515 52 in in IN cord-022235-6ircruag 515 53 the the DT cord-022235-6ircruag 515 54 TGN TGN NNP cord-022235-6ircruag 515 55 . . . cord-022235-6ircruag 516 1 This this DT cord-022235-6ircruag 516 2 idea idea NN cord-022235-6ircruag 516 3 is be VBZ cord-022235-6ircruag 516 4 incompatible incompatible JJ cord-022235-6ircruag 516 5 with with IN cord-022235-6ircruag 516 6 the the DT cord-022235-6ircruag 516 7 observation observation NN cord-022235-6ircruag 516 8 that that IN cord-022235-6ircruag 516 9 some some DT cord-022235-6ircruag 516 10 lysosomal lysosomal JJ cord-022235-6ircruag 516 11 proteins protein NNS cord-022235-6ircruag 516 12 are be VBP cord-022235-6ircruag 516 13 terminally terminally RB cord-022235-6ircruag 516 14 pro pro JJ cord-022235-6ircruag 516 15 cessed cesse VBN cord-022235-6ircruag 516 16 by by IN cord-022235-6ircruag 516 17 enzymes enzyme NNS cord-022235-6ircruag 516 18 located locate VBN cord-022235-6ircruag 516 19 in in IN cord-022235-6ircruag 516 20 medial medial JJ cord-022235-6ircruag 516 21 and and CC cord-022235-6ircruag 516 22 trans trans NNP cord-022235-6ircruag 516 23 Golgi Golgi NNP cord-022235-6ircruag 516 24 compartments compartment NNS cord-022235-6ircruag 516 25 . . . cord-022235-6ircruag 517 1 Other other JJ cord-022235-6ircruag 517 2 cytological cytological JJ cord-022235-6ircruag 517 3 studies study NNS cord-022235-6ircruag 517 4 indicate indicate VBP cord-022235-6ircruag 517 5 that that IN cord-022235-6ircruag 517 6 M6P m6p NN cord-022235-6ircruag 517 7 receptors receptor NNS cord-022235-6ircruag 517 8 are be VBP cord-022235-6ircruag 517 9 located locate VBN cord-022235-6ircruag 517 10 in in IN cord-022235-6ircruag 517 11 the the DT cord-022235-6ircruag 517 12 TGN TGN NNP cord-022235-6ircruag 517 13 , , , cord-022235-6ircruag 517 14 as as RB cord-022235-6ircruag 517 15 well well RB cord-022235-6ircruag 517 16 as as IN cord-022235-6ircruag 517 17 throughout throughout IN cord-022235-6ircruag 517 18 the the DT cord-022235-6ircruag 517 19 Golgi Golgi NNP cord-022235-6ircruag 517 20 stack stack NN cord-022235-6ircruag 517 21 ( ( -LRB- cord-022235-6ircruag 517 22 208 208 CD cord-022235-6ircruag 517 23 ) ) -RRB- cord-022235-6ircruag 517 24 , , , cord-022235-6ircruag 517 25 in in IN cord-022235-6ircruag 517 26 coated coated JJ cord-022235-6ircruag 517 27 vesicles vesicle NNS cord-022235-6ircruag 517 28 and and CC cord-022235-6ircruag 517 29 the the DT cord-022235-6ircruag 517 30 plasma plasma NN cord-022235-6ircruag 517 31 membrane membrane NN cord-022235-6ircruag 517 32 ( ( -LRB- cord-022235-6ircruag 517 33 365,366 365,366 CD cord-022235-6ircruag 517 34 ) ) -RRB- cord-022235-6ircruag 517 35 ( ( -LRB- cord-022235-6ircruag 517 36 see see VB cord-022235-6ircruag 517 37 below below RB cord-022235-6ircruag 517 38 for for IN cord-022235-6ircruag 517 39 explanation explanation NN cord-022235-6ircruag 517 40 ) ) -RRB- cord-022235-6ircruag 517 41 , , , cord-022235-6ircruag 517 42 and and CC cord-022235-6ircruag 517 43 in in IN cord-022235-6ircruag 517 44 a a DT cord-022235-6ircruag 517 45 Golgi Golgi NNP cord-022235-6ircruag 517 46 - - HYPH cord-022235-6ircruag 517 47 proximal proximal JJ cord-022235-6ircruag 517 48 vesicular vesicular JJ cord-022235-6ircruag 517 49 structure structure NN cord-022235-6ircruag 517 50 ( ( -LRB- cord-022235-6ircruag 517 51 402 402 CD cord-022235-6ircruag 517 52 ) ) -RRB- cord-022235-6ircruag 517 53 , , , cord-022235-6ircruag 517 54 but but CC cord-022235-6ircruag 517 55 not not RB cord-022235-6ircruag 517 56 in in IN cord-022235-6ircruag 517 57 lysosomes lysosome NNS cord-022235-6ircruag 517 58 . . . cord-022235-6ircruag 518 1 Thus thus RB cord-022235-6ircruag 518 2 , , , cord-022235-6ircruag 518 3 ly ly NNP cord-022235-6ircruag 518 4 sosomal sosomal NNP cord-022235-6ircruag 518 5 enzymes enzyme NNS cord-022235-6ircruag 518 6 probably probably RB cord-022235-6ircruag 518 7 do do VBP cord-022235-6ircruag 518 8 transit transit NN cord-022235-6ircruag 518 9 through through IN cord-022235-6ircruag 518 10 the the DT cord-022235-6ircruag 518 11 TGN TGN NNP cord-022235-6ircruag 518 12 , , , cord-022235-6ircruag 518 13 possibly possibly RB cord-022235-6ircruag 518 14 already already RB cord-022235-6ircruag 518 15 complexed complexed JJ cord-022235-6ircruag 518 16 with with IN cord-022235-6ircruag 518 17 their -PRON- PRP$ cord-022235-6ircruag 518 18 receptor receptor NN cord-022235-6ircruag 518 19 , , , cord-022235-6ircruag 518 20 although although IN cord-022235-6ircruag 518 21 Farquhar Farquhar NNP cord-022235-6ircruag 518 22 ( ( -LRB- cord-022235-6ircruag 518 23 299 299 CD cord-022235-6ircruag 518 24 ) ) -RRB- cord-022235-6ircruag 518 25 argues argue VBZ cord-022235-6ircruag 518 26 strongly strongly RB cord-022235-6ircruag 518 27 in in IN cord-022235-6ircruag 518 28 favor favor NN cord-022235-6ircruag 518 29 of of IN cord-022235-6ircruag 518 30 multiple multiple JJ cord-022235-6ircruag 518 31 lysosomal lysosomal JJ cord-022235-6ircruag 518 32 sorting sort VBG cord-022235-6ircruag 518 33 pathways pathway NNS cord-022235-6ircruag 518 34 and and CC cord-022235-6ircruag 518 35 in in IN cord-022235-6ircruag 518 36 particular particular JJ cord-022235-6ircruag 518 37 for for IN cord-022235-6ircruag 518 38 sorting sort VBG cord-022235-6ircruag 518 39 from from IN cord-022235-6ircruag 518 40 the the DT cord-022235-6ircruag 518 41 cis cis NNP cord-022235-6ircruag 518 42 Golgi Golgi NNP cord-022235-6ircruag 518 43 in in IN cord-022235-6ircruag 518 44 certain certain JJ cord-022235-6ircruag 518 45 cell cell NN cord-022235-6ircruag 518 46 lines line NNS cord-022235-6ircruag 518 47 . . . cord-022235-6ircruag 519 1 Thus thus RB cord-022235-6ircruag 519 2 , , , cord-022235-6ircruag 519 3 the the DT cord-022235-6ircruag 519 4 site site NN cord-022235-6ircruag 519 5 of of IN cord-022235-6ircruag 519 6 accumulation accumulation NN cord-022235-6ircruag 519 7 of of IN cord-022235-6ircruag 519 8 M6P m6p NN cord-022235-6ircruag 519 9 receptors receptor NNS cord-022235-6ircruag 519 10 along along IN cord-022235-6ircruag 519 11 the the DT cord-022235-6ircruag 519 12 secretory secretory JJ cord-022235-6ircruag 519 13 pathway pathway NN cord-022235-6ircruag 519 14 may may MD cord-022235-6ircruag 519 15 have have VB cord-022235-6ircruag 519 16 little little JJ cord-022235-6ircruag 519 17 relevance relevance NN cord-022235-6ircruag 519 18 for for IN cord-022235-6ircruag 519 19 lysosomal lysosomal JJ cord-022235-6ircruag 519 20 enzyme enzyme NN cord-022235-6ircruag 519 21 sorting sorting NN cord-022235-6ircruag 519 22 . . . cord-022235-6ircruag 520 1 Two two CD cord-022235-6ircruag 520 2 M6P m6p NN cord-022235-6ircruag 520 3 receptors receptor NNS cord-022235-6ircruag 520 4 ( ( -LRB- cord-022235-6ircruag 520 5 275 275 CD cord-022235-6ircruag 520 6 kDa kDa NNS cord-022235-6ircruag 520 7 and and CC cord-022235-6ircruag 520 8 46 46 CD cord-022235-6ircruag 520 9 kDa kDa NNS cord-022235-6ircruag 520 10 ) ) -RRB- cord-022235-6ircruag 520 11 have have VBP cord-022235-6ircruag 520 12 been be VBN cord-022235-6ircruag 520 13 identified identify VBN cord-022235-6ircruag 520 14 and and CC cord-022235-6ircruag 520 15 characterized characterize VBN cord-022235-6ircruag 520 16 . . . cord-022235-6ircruag 521 1 The the DT cord-022235-6ircruag 521 2 receptor receptor NN cord-022235-6ircruag 521 3 activity activity NN cord-022235-6ircruag 521 4 of of IN cord-022235-6ircruag 521 5 the the DT cord-022235-6ircruag 521 6 275-kDa 275-kda CD cord-022235-6ircruag 521 7 protein protein NN cord-022235-6ircruag 521 8 , , , cord-022235-6ircruag 521 9 but but CC cord-022235-6ircruag 521 10 not not RB cord-022235-6ircruag 521 11 that that DT cord-022235-6ircruag 521 12 of of IN cord-022235-6ircruag 521 13 the the DT cord-022235-6ircruag 521 14 46-kDa 46-kda CD cord-022235-6ircruag 521 15 protein protein NN cord-022235-6ircruag 521 16 is be VBZ cord-022235-6ircruag 521 17 cation cation NN cord-022235-6ircruag 521 18 - - HYPH cord-022235-6ircruag 521 19 independent independent JJ cord-022235-6ircruag 521 20 , , , cord-022235-6ircruag 521 21 and and CC cord-022235-6ircruag 521 22 the the DT cord-022235-6ircruag 521 23 46-kDa 46-kda CD cord-022235-6ircruag 521 24 receptor receptor NN cord-022235-6ircruag 521 25 recognizes recognize VBZ cord-022235-6ircruag 521 26 only only JJ cord-022235-6ircruag 521 27 phosphomonoesters phosphomonoester NNS cord-022235-6ircruag 521 28 whereas whereas IN cord-022235-6ircruag 521 29 the the DT cord-022235-6ircruag 521 30 275-kDa 275-kda CD cord-022235-6ircruag 521 31 protein protein NN cord-022235-6ircruag 521 32 also also RB cord-022235-6ircruag 521 33 binds bind VBZ cord-022235-6ircruag 521 34 methylphosphomannosyl methylphosphomannosyl NN cord-022235-6ircruag 521 35 residues residue NNS cord-022235-6ircruag 521 36 ( ( -LRB- cord-022235-6ircruag 521 37 461 461 CD cord-022235-6ircruag 521 38 ) ) -RRB- cord-022235-6ircruag 521 39 . . . cord-022235-6ircruag 522 1 The the DT cord-022235-6ircruag 522 2 275-kDa 275-kda CD cord-022235-6ircruag 522 3 protein protein NN cord-022235-6ircruag 522 4 ap ap NNP cord-022235-6ircruag 522 5 pears pear NNS cord-022235-6ircruag 522 6 to to TO cord-022235-6ircruag 522 7 be be VB cord-022235-6ircruag 522 8 present present JJ cord-022235-6ircruag 522 9 in in IN cord-022235-6ircruag 522 10 most most JJS cord-022235-6ircruag 522 11 mammalian mammalian JJ cord-022235-6ircruag 522 12 cell cell NN cord-022235-6ircruag 522 13 lines line NNS cord-022235-6ircruag 522 14 tested test VBN cord-022235-6ircruag 522 15 so so RB cord-022235-6ircruag 522 16 far far RB cord-022235-6ircruag 522 17 ( ( -LRB- cord-022235-6ircruag 522 18 982 982 CD cord-022235-6ircruag 522 19 ) ) -RRB- cord-022235-6ircruag 522 20 , , , cord-022235-6ircruag 522 21 but but CC cord-022235-6ircruag 522 22 the the DT cord-022235-6ircruag 522 23 distribution distribution NN cord-022235-6ircruag 522 24 of of IN cord-022235-6ircruag 522 25 the the DT cord-022235-6ircruag 522 26 46-kDa 46-kda CD cord-022235-6ircruag 522 27 receptor receptor NN cord-022235-6ircruag 522 28 has have VBZ cord-022235-6ircruag 522 29 not not RB cord-022235-6ircruag 522 30 been be VBN cord-022235-6ircruag 522 31 determined determine VBN cord-022235-6ircruag 522 32 . . . cord-022235-6ircruag 523 1 Some some DT cord-022235-6ircruag 523 2 mutant mutant JJ cord-022235-6ircruag 523 3 cell cell NN cord-022235-6ircruag 523 4 lines line NNS cord-022235-6ircruag 523 5 lack lack VBP cord-022235-6ircruag 523 6 the the DT cord-022235-6ircruag 523 7 275-kDa 275-kda CD cord-022235-6ircruag 523 8 M6P m6p NN cord-022235-6ircruag 523 9 receptor receptor NN cord-022235-6ircruag 523 10 yet yet RB cord-022235-6ircruag 523 11 still still RB cord-022235-6ircruag 523 12 target target VB cord-022235-6ircruag 523 13 lysosomal lysosomal JJ cord-022235-6ircruag 523 14 enzymes enzyme NNS cord-022235-6ircruag 523 15 normally normally RB cord-022235-6ircruag 523 16 , , , cord-022235-6ircruag 523 17 which which WDT cord-022235-6ircruag 523 18 suggests suggest VBZ cord-022235-6ircruag 523 19 that that IN cord-022235-6ircruag 523 20 both both DT cord-022235-6ircruag 523 21 receptors receptor NNS cord-022235-6ircruag 523 22 are be VBP cord-022235-6ircruag 523 23 involved involve VBN cord-022235-6ircruag 523 24 in in IN cord-022235-6ircruag 523 25 lysosomal lysosomal JJ cord-022235-6ircruag 523 26 sorting sorting NN cord-022235-6ircruag 523 27 ( ( -LRB- cord-022235-6ircruag 523 28 461 461 CD cord-022235-6ircruag 523 29 ) ) -RRB- cord-022235-6ircruag 523 30 . . . cord-022235-6ircruag 524 1 Studies study NNS cord-022235-6ircruag 524 2 with with IN cord-022235-6ircruag 524 3 such such JJ cord-022235-6ircruag 524 4 cell cell NN cord-022235-6ircruag 524 5 lines line NNS cord-022235-6ircruag 524 6 revealed reveal VBD cord-022235-6ircruag 524 7 another another DT cord-022235-6ircruag 524 8 difference difference NN cord-022235-6ircruag 524 9 between between IN cord-022235-6ircruag 524 10 the the DT cord-022235-6ircruag 524 11 two two CD cord-022235-6ircruag 524 12 receptors receptor NNS cord-022235-6ircruag 524 13 , , , cord-022235-6ircruag 524 14 however however RB cord-022235-6ircruag 524 15 . . . cord-022235-6ircruag 525 1 Although although IN cord-022235-6ircruag 525 2 part part NN cord-022235-6ircruag 525 3 of of IN cord-022235-6ircruag 525 4 the the DT cord-022235-6ircruag 525 5 cellular cellular JJ cord-022235-6ircruag 525 6 pool pool NN cord-022235-6ircruag 525 7 of of IN cord-022235-6ircruag 525 8 both both DT cord-022235-6ircruag 525 9 receptors receptor NNS cord-022235-6ircruag 525 10 is be VBZ cord-022235-6ircruag 525 11 located locate VBN cord-022235-6ircruag 525 12 on on IN cord-022235-6ircruag 525 13 the the DT cord-022235-6ircruag 525 14 cell cell NN cord-022235-6ircruag 525 15 surface surface NN cord-022235-6ircruag 525 16 , , , cord-022235-6ircruag 525 17 only only RB cord-022235-6ircruag 525 18 cells cell NNS cord-022235-6ircruag 525 19 with with IN cord-022235-6ircruag 525 20 the the DT cord-022235-6ircruag 525 21 275-kDa 275-kda CD cord-022235-6ircruag 525 22 protein protein NN cord-022235-6ircruag 525 23 can can MD cord-022235-6ircruag 525 24 endocytose endocytose VB cord-022235-6ircruag 525 25 secreted secrete VBN cord-022235-6ircruag 525 26 lysosomal lysosomal JJ cord-022235-6ircruag 525 27 proteins protein NNS cord-022235-6ircruag 525 28 ( ( -LRB- cord-022235-6ircruag 525 29 1077 1077 CD cord-022235-6ircruag 525 30 ) ) -RRB- cord-022235-6ircruag 525 31 . . . cord-022235-6ircruag 526 1 This this DT cord-022235-6ircruag 526 2 " " `` cord-022235-6ircruag 526 3 defect defect NN cord-022235-6ircruag 526 4 " " '' cord-022235-6ircruag 526 5 in in IN cord-022235-6ircruag 526 6 the the DT cord-022235-6ircruag 526 7 46-kDa 46-kda CD cord-022235-6ircruag 527 1 protein protein NN cord-022235-6ircruag 527 2 appears appear VBZ cord-022235-6ircruag 527 3 to to TO cord-022235-6ircruag 527 4 be be VB cord-022235-6ircruag 527 5 due due JJ cord-022235-6ircruag 527 6 to to IN cord-022235-6ircruag 527 7 a a DT cord-022235-6ircruag 527 8 failure failure NN cord-022235-6ircruag 527 9 to to TO cord-022235-6ircruag 527 10 bind bind VB cord-022235-6ircruag 527 11 the the DT cord-022235-6ircruag 527 12 ligands ligand NNS cord-022235-6ircruag 527 13 , , , cord-022235-6ircruag 527 14 since since IN cord-022235-6ircruag 527 15 antibodies antibody NNS cord-022235-6ircruag 527 16 against against IN cord-022235-6ircruag 527 17 the the DT cord-022235-6ircruag 527 18 46-kDa 46-kda CD cord-022235-6ircruag 528 1 protein protein NN cord-022235-6ircruag 528 2 are be VBP cord-022235-6ircruag 528 3 endocytosed endocytose VBN cord-022235-6ircruag 528 4 normally normally RB cord-022235-6ircruag 528 5 ( ( -LRB- cord-022235-6ircruag 528 6 see see VB cord-022235-6ircruag 528 7 Chapter chapter NN cord-022235-6ircruag 528 8 VIII VIII NNP cord-022235-6ircruag 528 9 for for IN cord-022235-6ircruag 528 10 more more JJR cord-022235-6ircruag 528 11 details detail NNS cord-022235-6ircruag 528 12 on on IN cord-022235-6ircruag 528 13 endocytosis endocytosis NN cord-022235-6ircruag 528 14 ) ) -RRB- cord-022235-6ircruag 528 15 . . . cord-022235-6ircruag 529 1 Thus thus RB cord-022235-6ircruag 529 2 , , , cord-022235-6ircruag 529 3 the the DT cord-022235-6ircruag 529 4 46-kDa 46-kda CD cord-022235-6ircruag 529 5 protein protein NN cord-022235-6ircruag 529 6 seems seem VBZ cord-022235-6ircruag 529 7 to to TO cord-022235-6ircruag 529 8 be be VB cord-022235-6ircruag 529 9 specifically specifically RB cord-022235-6ircruag 529 10 involved involve VBN cord-022235-6ircruag 529 11 in in IN cord-022235-6ircruag 529 12 the the DT cord-022235-6ircruag 529 13 sorting sorting NN cord-022235-6ircruag 529 14 of of IN cord-022235-6ircruag 529 15 endogenous endogenous JJ cord-022235-6ircruag 529 16 lysosomal lysosomal JJ cord-022235-6ircruag 529 17 enzymes enzyme NNS cord-022235-6ircruag 529 18 . . . cord-022235-6ircruag 530 1 The the DT cord-022235-6ircruag 530 2 genes gene NNS cord-022235-6ircruag 530 3 for for IN cord-022235-6ircruag 530 4 both both DT cord-022235-6ircruag 530 5 M6P m6p NN cord-022235-6ircruag 530 6 receptors receptor NNS cord-022235-6ircruag 530 7 have have VBP cord-022235-6ircruag 530 8 been be VBN cord-022235-6ircruag 530 9 cloned clone VBN cord-022235-6ircruag 530 10 and and CC cord-022235-6ircruag 530 11 sequenced sequence VBN cord-022235-6ircruag 530 12 . . . cord-022235-6ircruag 531 1 Although although IN cord-022235-6ircruag 531 2 the the DT cord-022235-6ircruag 531 3 predicted predict VBN cord-022235-6ircruag 531 4 sequence sequence NN cord-022235-6ircruag 531 5 of of IN cord-022235-6ircruag 531 6 the the DT cord-022235-6ircruag 531 7 two two CD cord-022235-6ircruag 531 8 gene gene NN cord-022235-6ircruag 531 9 products product NNS cord-022235-6ircruag 531 10 are be VBP cord-022235-6ircruag 531 11 generally generally RB cord-022235-6ircruag 531 12 different different JJ cord-022235-6ircruag 531 13 , , , cord-022235-6ircruag 531 14 the the DT cord-022235-6ircruag 531 15 two two CD cord-022235-6ircruag 531 16 proteins protein NNS cord-022235-6ircruag 531 17 have have VBP cord-022235-6ircruag 531 18 a a DT cord-022235-6ircruag 531 19 region region NN cord-022235-6ircruag 531 20 of of IN cord-022235-6ircruag 531 21 moderate moderate JJ cord-022235-6ircruag 531 22 sequence sequence NN cord-022235-6ircruag 531 23 similarity similarity NN cord-022235-6ircruag 531 24 in in IN cord-022235-6ircruag 531 25 their -PRON- PRP$ cord-022235-6ircruag 531 26 lumenal lumenal JJ cord-022235-6ircruag 531 27 domains domain NNS cord-022235-6ircruag 531 28 ( ( -LRB- cord-022235-6ircruag 531 29 645,826 645,826 CD cord-022235-6ircruag 531 30 ) ) -RRB- cord-022235-6ircruag 531 31 which which WDT cord-022235-6ircruag 531 32 Dahms Dahms NNP cord-022235-6ircruag 531 33 et et NNP cord-022235-6ircruag 531 34 al al NNP cord-022235-6ircruag 531 35 . . . cord-022235-6ircruag 532 1 ( ( -LRB- cord-022235-6ircruag 532 2 208 208 CD cord-022235-6ircruag 532 3 ) ) -RRB- cord-022235-6ircruag 532 4 propose propose VB cord-022235-6ircruag 532 5 could could MD cord-022235-6ircruag 532 6 be be VB cord-022235-6ircruag 532 7 the the DT cord-022235-6ircruag 532 8 M6P m6p NN cord-022235-6ircruag 532 9 binding bind VBG cord-022235-6ircruag 532 10 domain domain NN cord-022235-6ircruag 532 11 . . . cord-022235-6ircruag 533 1 The the DT cord-022235-6ircruag 533 2 sorting sorting NN cord-022235-6ircruag 533 3 of of IN cord-022235-6ircruag 533 4 the the DT cord-022235-6ircruag 533 5 lysosomal lysosomal JJ cord-022235-6ircruag 533 6 enzymes enzyme NNS cord-022235-6ircruag 534 1 cathepsin cathepsin NNP cord-022235-6ircruag 534 2 C C NNP cord-022235-6ircruag 534 3 and and CC cord-022235-6ircruag 534 4 cathepsin cathepsin NN cord-022235-6ircruag 534 5 D D NNP cord-022235-6ircruag 534 6 was be VBD cord-022235-6ircruag 534 7 studied study VBN cord-022235-6ircruag 534 8 directly directly RB cord-022235-6ircruag 534 9 by by IN cord-022235-6ircruag 534 10 Lemansky Lemansky NNP cord-022235-6ircruag 534 11 et et NNP cord-022235-6ircruag 534 12 al al NNP cord-022235-6ircruag 534 13 . . . cord-022235-6ircruag 535 1 ( ( -LRB- cord-022235-6ircruag 535 2 626 626 CD cord-022235-6ircruag 535 3 ) ) -RRB- cord-022235-6ircruag 535 4 , , , cord-022235-6ircruag 535 5 who who WP cord-022235-6ircruag 535 6 found find VBD cord-022235-6ircruag 535 7 that that IN cord-022235-6ircruag 535 8 lysosomal lysosomal JJ cord-022235-6ircruag 535 9 enzyme enzyme NN cord-022235-6ircruag 535 10 precursors precursor NNS cord-022235-6ircruag 535 11 occurred occur VBD cord-022235-6ircruag 535 12 only only RB cord-022235-6ircruag 535 13 in in IN cord-022235-6ircruag 535 14 coated coated JJ cord-022235-6ircruag 535 15 vesicles vesicle NNS cord-022235-6ircruag 535 16 . . . cord-022235-6ircruag 536 1 Proteolytically Proteolytically NNP cord-022235-6ircruag 536 2 ma ma NNP cord-022235-6ircruag 536 3 tured ture VBN cord-022235-6ircruag 536 4 forms form NNS cord-022235-6ircruag 536 5 were be VBD cord-022235-6ircruag 536 6 found find VBN cord-022235-6ircruag 536 7 in in IN cord-022235-6ircruag 536 8 lysosomes lysosome NNS cord-022235-6ircruag 536 9 . . . cord-022235-6ircruag 537 1 Schulze Schulze NNP cord-022235-6ircruag 537 2 - - HYPH cord-022235-6ircruag 537 3 Lohoff Lohoff NNP cord-022235-6ircruag 537 4 et et FW cord-022235-6ircruag 537 5 al al NNP cord-022235-6ircruag 537 6 . . . cord-022235-6ircruag 538 1 ( ( -LRB- cord-022235-6ircruag 538 2 1022 1022 CD cord-022235-6ircruag 538 3 ) ) -RRB- cord-022235-6ircruag 538 4 also also RB cord-022235-6ircruag 538 5 observed observe VBD cord-022235-6ircruag 538 6 the the DT cord-022235-6ircruag 538 7 transient transient JJ cord-022235-6ircruag 538 8 accumulation accumulation NN cord-022235-6ircruag 538 9 of of IN cord-022235-6ircruag 538 10 one one CD cord-022235-6ircruag 538 11 of of IN cord-022235-6ircruag 538 12 these these DT cord-022235-6ircruag 538 13 enzymes enzyme NNS cord-022235-6ircruag 538 14 in in IN cord-022235-6ircruag 538 15 coated coated JJ cord-022235-6ircruag 538 16 vesicles vesicle NNS cord-022235-6ircruag 538 17 , , , cord-022235-6ircruag 538 18 which which WDT cord-022235-6ircruag 538 19 , , , cord-022235-6ircruag 538 20 they -PRON- PRP cord-022235-6ircruag 538 21 proposed propose VBD cord-022235-6ircruag 538 22 , , , cord-022235-6ircruag 538 23 are be VBP cord-022235-6ircruag 538 24 specifically specifically RB cord-022235-6ircruag 538 25 involved involve VBN cord-022235-6ircruag 538 26 in in IN cord-022235-6ircruag 538 27 the the DT cord-022235-6ircruag 538 28 sorting sorting NN cord-022235-6ircruag 538 29 of of IN cord-022235-6ircruag 538 30 lysosomal lysosomal JJ cord-022235-6ircruag 538 31 proteins protein NNS cord-022235-6ircruag 538 32 from from IN cord-022235-6ircruag 538 33 the the DT cord-022235-6ircruag 538 34 secretory secretory JJ cord-022235-6ircruag 538 35 pathway pathway NN cord-022235-6ircruag 538 36 . . . cord-022235-6ircruag 539 1 Lemansky Lemansky NNP cord-022235-6ircruag 539 2 et et NNP cord-022235-6ircruag 539 3 al al NNP cord-022235-6ircruag 539 4 . . . cord-022235-6ircruag 540 1 ( ( -LRB- cord-022235-6ircruag 540 2 626 626 CD cord-022235-6ircruag 540 3 ) ) -RRB- cord-022235-6ircruag 540 4 devised devise VBN cord-022235-6ircruag 540 5 procedures procedure NNS cord-022235-6ircruag 540 6 which which WDT cord-022235-6ircruag 540 7 allowed allow VBD cord-022235-6ircruag 540 8 vesicles vesicle NNS cord-022235-6ircruag 540 9 derived derive VBN cord-022235-6ircruag 540 10 from from IN cord-022235-6ircruag 540 11 the the DT cord-022235-6ircruag 540 12 secretory secretory JJ cord-022235-6ircruag 540 13 pathway pathway NN cord-022235-6ircruag 540 14 to to TO cord-022235-6ircruag 540 15 be be VB cord-022235-6ircruag 540 16 separated separate VBN cord-022235-6ircruag 540 17 from from IN cord-022235-6ircruag 540 18 those those DT cord-022235-6ircruag 540 19 derived derive VBN cord-022235-6ircruag 540 20 from from IN cord-022235-6ircruag 540 21 the the DT cord-022235-6ircruag 540 22 endocytotic endocytotic JJ cord-022235-6ircruag 540 23 path path NN cord-022235-6ircruag 540 24 way way NN cord-022235-6ircruag 540 25 . . . cord-022235-6ircruag 541 1 They -PRON- PRP cord-022235-6ircruag 541 2 found find VBD cord-022235-6ircruag 541 3 that that IN cord-022235-6ircruag 541 4 both both DT cord-022235-6ircruag 541 5 classes class NNS cord-022235-6ircruag 541 6 of of IN cord-022235-6ircruag 541 7 vesicles vesicle NNS cord-022235-6ircruag 541 8 contained contain VBD cord-022235-6ircruag 541 9 precursor precursor NN cord-022235-6ircruag 541 10 forms form NNS cord-022235-6ircruag 541 11 of of IN cord-022235-6ircruag 541 12 cathepsin cathepsin NN cord-022235-6ircruag 541 13 . . . cord-022235-6ircruag 542 1 These these DT cord-022235-6ircruag 542 2 studies study NNS cord-022235-6ircruag 542 3 have have VBP cord-022235-6ircruag 542 4 two two CD cord-022235-6ircruag 542 5 profound profound JJ cord-022235-6ircruag 542 6 implications implication NNS cord-022235-6ircruag 542 7 : : : cord-022235-6ircruag 542 8 ( ( -LRB- cord-022235-6ircruag 542 9 i i NN cord-022235-6ircruag 542 10 ) ) -RRB- cord-022235-6ircruag 543 1 The the DT cord-022235-6ircruag 543 2 fact fact NN cord-022235-6ircruag 543 3 that that IN cord-022235-6ircruag 543 4 vesicles vesicle NNS cord-022235-6ircruag 543 5 involved involve VBN cord-022235-6ircruag 543 6 in in IN cord-022235-6ircruag 543 7 direct direct JJ cord-022235-6ircruag 543 8 sorting sorting NN cord-022235-6ircruag 543 9 of of IN cord-022235-6ircruag 543 10 cathepsins cathepsin NNS cord-022235-6ircruag 543 11 to to IN cord-022235-6ircruag 543 12 the the DT cord-022235-6ircruag 543 13 lysosome lysosome NN cord-022235-6ircruag 543 14 contain contain VBP cord-022235-6ircruag 543 15 clathrin clathrin NN cord-022235-6ircruag 543 16 implies imply VBZ cord-022235-6ircruag 543 17 that that IN cord-022235-6ircruag 543 18 they -PRON- PRP cord-022235-6ircruag 543 19 had have VBD cord-022235-6ircruag 543 20 passed pass VBN cord-022235-6ircruag 543 21 through through IN cord-022235-6ircruag 543 22 the the DT cord-022235-6ircruag 543 23 TGN TGN NNP cord-022235-6ircruag 543 24 , , , cord-022235-6ircruag 543 25 the the DT cord-022235-6ircruag 543 26 first first JJ cord-022235-6ircruag 543 27 site site NN cord-022235-6ircruag 543 28 along along IN cord-022235-6ircruag 543 29 the the DT cord-022235-6ircruag 543 30 secretory secretory JJ cord-022235-6ircruag 543 31 pathway pathway NN cord-022235-6ircruag 543 32 at at IN cord-022235-6ircruag 543 33 which which WDT cord-022235-6ircruag 543 34 clathrin clathrin NNP cord-022235-6ircruag 543 35 is be VBZ cord-022235-6ircruag 543 36 detected detect VBN cord-022235-6ircruag 543 37 ( ( -LRB- cord-022235-6ircruag 543 38 Section Section NNP cord-022235-6ircruag 543 39 V.G.I V.G.I NNP cord-022235-6ircruag 543 40 ) ) -RRB- cord-022235-6ircruag 543 41 . . . cord-022235-6ircruag 544 1 ( ( -LRB- cord-022235-6ircruag 544 2 ii ii NN cord-022235-6ircruag 544 3 ) ) -RRB- cord-022235-6ircruag 545 1 The the DT cord-022235-6ircruag 545 2 fact fact NN cord-022235-6ircruag 545 3 that that IN cord-022235-6ircruag 545 4 some some DT cord-022235-6ircruag 545 5 lysosomal lysosomal JJ cord-022235-6ircruag 545 6 enzymes enzyme NNS cord-022235-6ircruag 545 7 are be VBP cord-022235-6ircruag 545 8 " " `` cord-022235-6ircruag 545 9 fished fish VBN cord-022235-6ircruag 545 10 " " '' cord-022235-6ircruag 545 11 out out IN cord-022235-6ircruag 545 12 of of IN cord-022235-6ircruag 545 13 the the DT cord-022235-6ircruag 545 14 sur sur FW cord-022235-6ircruag 545 15 rounding round VBG cord-022235-6ircruag 545 16 medium medium NN cord-022235-6ircruag 545 17 and and CC cord-022235-6ircruag 545 18 retargeted retargete VBN cord-022235-6ircruag 545 19 to to IN cord-022235-6ircruag 545 20 the the DT cord-022235-6ircruag 545 21 lysosome lysosome NN cord-022235-6ircruag 545 22 implies imply VBZ cord-022235-6ircruag 545 23 that that IN cord-022235-6ircruag 545 24 some some DT cord-022235-6ircruag 545 25 lysosomal lysosomal JJ cord-022235-6ircruag 545 26 enzymes enzyme NNS cord-022235-6ircruag 545 27 are be VBP cord-022235-6ircruag 545 28 incorrectly incorrectly RB cord-022235-6ircruag 545 29 sorted sort VBN cord-022235-6ircruag 545 30 , , , cord-022235-6ircruag 545 31 probably probably RB cord-022235-6ircruag 545 32 into into IN cord-022235-6ircruag 545 33 the the DT cord-022235-6ircruag 545 34 consti consti FW cord-022235-6ircruag 545 35 tutive tutive NNP cord-022235-6ircruag 545 36 secretory secretory NNP cord-022235-6ircruag 545 37 pathway pathway NNP cord-022235-6ircruag 545 38 ( ( -LRB- cord-022235-6ircruag 545 39 see see VB cord-022235-6ircruag 545 40 also also RB cord-022235-6ircruag 545 41 Section Section NNP cord-022235-6ircruag 545 42 V.D. V.D. NNP cord-022235-6ircruag 545 43 1 1 CD cord-022235-6ircruag 545 44 .a .a CD cord-022235-6ircruag 545 45 ) ) -RRB- cord-022235-6ircruag 545 46 . . . cord-022235-6ircruag 546 1 Higher high JJR cord-022235-6ircruag 546 2 levels level NNS cord-022235-6ircruag 546 3 of of IN cord-022235-6ircruag 546 4 incorrect incorrect JJ cord-022235-6ircruag 546 5 sorting sorting NN cord-022235-6ircruag 546 6 occurs occur VBZ cord-022235-6ircruag 546 7 in in IN cord-022235-6ircruag 546 8 NH NH NNP cord-022235-6ircruag 546 9 4 4 CD cord-022235-6ircruag 546 10 -Cl -Cl VBD cord-022235-6ircruag 546 11 - - HYPH cord-022235-6ircruag 546 12 treated treat VBN cord-022235-6ircruag 546 13 cells cell NNS cord-022235-6ircruag 546 14 , , , cord-022235-6ircruag 546 15 probably probably RB cord-022235-6ircruag 546 16 because because IN cord-022235-6ircruag 546 17 low low JJ cord-022235-6ircruag 546 18 pH pH NNP cord-022235-6ircruag 546 19 is be VBZ cord-022235-6ircruag 546 20 required require VBN cord-022235-6ircruag 546 21 to to TO cord-022235-6ircruag 546 22 dissociate dissociate VB cord-022235-6ircruag 546 23 M6P m6p NN cord-022235-6ircruag 546 24 from from IN cord-022235-6ircruag 546 25 its -PRON- PRP$ cord-022235-6ircruag 546 26 receptor receptor NN cord-022235-6ircruag 546 27 in in IN cord-022235-6ircruag 546 28 the the DT cord-022235-6ircruag 546 29 prelysosome prelysosome NN cord-022235-6ircruag 547 1 ( ( -LRB- cord-022235-6ircruag 547 2 Another another DT cord-022235-6ircruag 547 3 interesting interesting JJ cord-022235-6ircruag 547 4 observation observation NN cord-022235-6ircruag 547 5 concerning concern VBG cord-022235-6ircruag 547 6 the the DT cord-022235-6ircruag 547 7 M6P M6P NNP cord-022235-6ircruag 547 8 receptor receptor NN cord-022235-6ircruag 547 9 is be VBZ cord-022235-6ircruag 547 10 that that IN cord-022235-6ircruag 547 11 it -PRON- PRP cord-022235-6ircruag 547 12 specifically specifically RB cord-022235-6ircruag 547 13 binds bind VBZ cord-022235-6ircruag 547 14 to to IN cord-022235-6ircruag 547 15 one one CD cord-022235-6ircruag 547 16 of of IN cord-022235-6ircruag 547 17 the the DT cord-022235-6ircruag 547 18 components component NNS cord-022235-6ircruag 547 19 ( ( -LRB- cord-022235-6ircruag 547 20 the the DT cord-022235-6ircruag 547 21 100-kDa 100-kda CD cord-022235-6ircruag 547 22 accessory accessory NN cord-022235-6ircruag 547 23 pro pro NNP cord-022235-6ircruag 547 24 tein tein NNP cord-022235-6ircruag 547 25 ) ) -RRB- cord-022235-6ircruag 547 26 of of IN cord-022235-6ircruag 547 27 the the DT cord-022235-6ircruag 547 28 clathrin clathrin NN cord-022235-6ircruag 547 29 cage cage NN cord-022235-6ircruag 547 30 which which WDT cord-022235-6ircruag 547 31 coats coat VBZ cord-022235-6ircruag 547 32 the the DT cord-022235-6ircruag 547 33 sorting sorting NN cord-022235-6ircruag 547 34 vesicles vesicle NNS cord-022235-6ircruag 547 35 ( ( -LRB- cord-022235-6ircruag 547 36 854 854 CD cord-022235-6ircruag 547 37 ) ) -RRB- cord-022235-6ircruag 547 38 ( ( -LRB- cord-022235-6ircruag 547 39 see see UH cord-022235-6ircruag 547 40 be be VB cord-022235-6ircruag 547 41 low low JJ cord-022235-6ircruag 547 42 ) ) -RRB- cord-022235-6ircruag 547 43 . . . cord-022235-6ircruag 548 1 This this DT cord-022235-6ircruag 548 2 may may MD cord-022235-6ircruag 548 3 have have VB cord-022235-6ircruag 548 4 particular particular JJ cord-022235-6ircruag 548 5 relevance relevance NN cord-022235-6ircruag 548 6 for for IN cord-022235-6ircruag 548 7 the the DT cord-022235-6ircruag 548 8 sorting sorting NN cord-022235-6ircruag 548 9 of of IN cord-022235-6ircruag 548 10 lysosomal lysosomal JJ cord-022235-6ircruag 548 11 enzymes enzyme NNS cord-022235-6ircruag 548 12 because because IN cord-022235-6ircruag 548 13 different different JJ cord-022235-6ircruag 548 14 classes class NNS cord-022235-6ircruag 548 15 of of IN cord-022235-6ircruag 548 16 clathrin clathrin NN cord-022235-6ircruag 548 17 - - HYPH cord-022235-6ircruag 548 18 coated coat VBN cord-022235-6ircruag 548 19 vesicles vesicle NNS cord-022235-6ircruag 548 20 appear appear VBP cord-022235-6ircruag 548 21 to to TO cord-022235-6ircruag 548 22 have have VB cord-022235-6ircruag 548 23 different different JJ cord-022235-6ircruag 548 24 types type NNS cord-022235-6ircruag 548 25 of of IN cord-022235-6ircruag 548 26 accessory accessory NN cord-022235-6ircruag 548 27 proteins protein NNS cord-022235-6ircruag 548 28 ( ( -LRB- cord-022235-6ircruag 548 29 855 855 CD cord-022235-6ircruag 548 30 ) ) -RRB- cord-022235-6ircruag 548 31 . . . cord-022235-6ircruag 549 1 Coated coated JJ cord-022235-6ircruag 549 2 vesicles vesicle NNS cord-022235-6ircruag 549 3 almost almost RB cord-022235-6ircruag 549 4 certainly certainly RB cord-022235-6ircruag 549 5 do do VBP cord-022235-6ircruag 549 6 not not RB cord-022235-6ircruag 549 7 transport transport VB cord-022235-6ircruag 549 8 proteins protein NNS cord-022235-6ircruag 549 9 directly directly RB cord-022235-6ircruag 549 10 into into IN cord-022235-6ircruag 549 11 lysosomes lysosome NNS cord-022235-6ircruag 549 12 . . . cord-022235-6ircruag 550 1 Instead instead RB cord-022235-6ircruag 550 2 , , , cord-022235-6ircruag 550 3 the the DT cord-022235-6ircruag 550 4 vesicles vesicle NNS cord-022235-6ircruag 550 5 are be VBP cord-022235-6ircruag 550 6 targeted target VBN cord-022235-6ircruag 550 7 to to IN cord-022235-6ircruag 550 8 endosome endosome NN cord-022235-6ircruag 550 9 - - HYPH cord-022235-6ircruag 550 10 like like JJ cord-022235-6ircruag 550 11 reticular reticular JJ cord-022235-6ircruag 550 12 organelles organelle NNS cord-022235-6ircruag 550 13 ( ( -LRB- cord-022235-6ircruag 550 14 prelysosomes prelysosomes NNP cord-022235-6ircruag 550 15 or or CC cord-022235-6ircruag 550 16 secondary secondary JJ cord-022235-6ircruag 550 17 endosomes endosome NNS cord-022235-6ircruag 550 18 ) ) -RRB- cord-022235-6ircruag 550 19 , , , cord-022235-6ircruag 550 20 where where WRB cord-022235-6ircruag 550 21 the the DT cord-022235-6ircruag 550 22 receptor receptor NN cord-022235-6ircruag 550 23 probably probably RB cord-022235-6ircruag 550 24 dissociates dissociate VBZ cord-022235-6ircruag 550 25 and and CC cord-022235-6ircruag 550 26 recycles recycle VBZ cord-022235-6ircruag 550 27 back back RB cord-022235-6ircruag 550 28 to to IN cord-022235-6ircruag 550 29 the the DT cord-022235-6ircruag 550 30 Golgi Golgi NNP cord-022235-6ircruag 550 31 cisternae cisternae NNP cord-022235-6ircruag 550 32 . . . cord-022235-6ircruag 551 1 This this DT cord-022235-6ircruag 551 2 organ organ NN cord-022235-6ircruag 551 3 elle elle NNP cord-022235-6ircruag 551 4 is be VBZ cord-022235-6ircruag 551 5 also also RB cord-022235-6ircruag 551 6 the the DT cord-022235-6ircruag 551 7 site site NN cord-022235-6ircruag 551 8 to to TO cord-022235-6ircruag 551 9 which which WDT cord-022235-6ircruag 551 10 endocytosed endocytosed JJ cord-022235-6ircruag 551 11 lysosomal lysosomal JJ cord-022235-6ircruag 551 12 proteins protein NNS cord-022235-6ircruag 551 13 are be VBP cord-022235-6ircruag 551 14 targeted target VBN cord-022235-6ircruag 551 15 ( ( -LRB- cord-022235-6ircruag 551 16 134,402 134,402 CD cord-022235-6ircruag 551 17 ) ) -RRB- cord-022235-6ircruag 551 18 ( ( -LRB- cord-022235-6ircruag 552 1 Fig Fig NNP cord-022235-6ircruag 552 2 . . . cord-022235-6ircruag 553 1 V.6 V.6 NNP cord-022235-6ircruag 553 2 and and CC cord-022235-6ircruag 553 3 Section section NN cord-022235-6ircruag 553 4 VIII VIII NNP cord-022235-6ircruag 553 5 . . . cord-022235-6ircruag 554 1 A.4.b A.4.b NNP cord-022235-6ircruag 554 2 ) ) -RRB- cord-022235-6ircruag 554 3 . . . cord-022235-6ircruag 555 1 A a DT cord-022235-6ircruag 555 2 different different JJ cord-022235-6ircruag 555 3 class class NN cord-022235-6ircruag 555 4 of of IN cord-022235-6ircruag 555 5 vesicles vesicle NNS cord-022235-6ircruag 555 6 may may MD cord-022235-6ircruag 555 7 complete complete VB cord-022235-6ircruag 555 8 the the DT cord-022235-6ircruag 555 9 transport transport NN cord-022235-6ircruag 555 10 of of IN cord-022235-6ircruag 555 11 lysosomal lysosomal JJ cord-022235-6ircruag 555 12 enzymes enzyme NNS cord-022235-6ircruag 555 13 once once IN cord-022235-6ircruag 555 14 they -PRON- PRP cord-022235-6ircruag 555 15 have have VBP cord-022235-6ircruag 555 16 dissoci dissoci NN cord-022235-6ircruag 555 17 ated ate VBN cord-022235-6ircruag 555 18 from from IN cord-022235-6ircruag 555 19 their -PRON- PRP$ cord-022235-6ircruag 555 20 receptor receptor NN cord-022235-6ircruag 555 21 , , , cord-022235-6ircruag 555 22 but but CC cord-022235-6ircruag 555 23 von von NNP cord-022235-6ircruag 555 24 Figura Figura NNP cord-022235-6ircruag 555 25 and and CC cord-022235-6ircruag 555 26 Hasilik Hasilik NNP cord-022235-6ircruag 555 27 ( ( -LRB- cord-022235-6ircruag 555 28 313 313 CD cord-022235-6ircruag 555 29 ) ) -RRB- cord-022235-6ircruag 555 30 consider consider VB cord-022235-6ircruag 555 31 it -PRON- PRP cord-022235-6ircruag 555 32 more more RBR cord-022235-6ircruag 555 33 likely likely JJ cord-022235-6ircruag 555 34 that that IN cord-022235-6ircruag 555 35 there there EX cord-022235-6ircruag 555 36 is be VBZ cord-022235-6ircruag 555 37 a a DT cord-022235-6ircruag 555 38 gradual gradual JJ cord-022235-6ircruag 555 39 transition transition NN cord-022235-6ircruag 555 40 from from IN cord-022235-6ircruag 555 41 tubular tubular JJ cord-022235-6ircruag 555 42 prelysosomes prelysosome NNS cord-022235-6ircruag 555 43 to to TO cord-022235-6ircruag 555 44 lyso lyso NNP cord-022235-6ircruag 555 45 somes somes NNP cord-022235-6ircruag 555 46 proper proper JJ cord-022235-6ircruag 555 47 . . . cord-022235-6ircruag 556 1 Although although IN cord-022235-6ircruag 556 2 M6P M6P NNP cord-022235-6ircruag 556 3 is be VBZ cord-022235-6ircruag 556 4 undoubtedly undoubtedly RB cord-022235-6ircruag 556 5 the the DT cord-022235-6ircruag 556 6 major major JJ cord-022235-6ircruag 556 7 , , , cord-022235-6ircruag 556 8 and and CC cord-022235-6ircruag 556 9 in in IN cord-022235-6ircruag 556 10 some some DT cord-022235-6ircruag 556 11 cells cell NNS cord-022235-6ircruag 556 12 the the DT cord-022235-6ircruag 556 13 only only RB cord-022235-6ircruag 556 14 , , , cord-022235-6ircruag 556 15 sorting sort VBG cord-022235-6ircruag 556 16 signal signal NN cord-022235-6ircruag 556 17 on on IN cord-022235-6ircruag 556 18 lysosomal lysosomal JJ cord-022235-6ircruag 556 19 enzymes enzyme NNS cord-022235-6ircruag 556 20 , , , cord-022235-6ircruag 556 21 some some DT cord-022235-6ircruag 556 22 lysosomal lysosomal JJ cord-022235-6ircruag 556 23 proteins protein NNS cord-022235-6ircruag 556 24 do do VBP cord-022235-6ircruag 556 25 not not RB cord-022235-6ircruag 556 26 have have VB cord-022235-6ircruag 556 27 M6P m6p NN cord-022235-6ircruag 556 28 residues residue NNS cord-022235-6ircruag 556 29 . . . cord-022235-6ircruag 557 1 Owada Owada NNP cord-022235-6ircruag 557 2 and and CC cord-022235-6ircruag 557 3 Neufeld Neufeld NNP cord-022235-6ircruag 557 4 ( ( -LRB- cord-022235-6ircruag 557 5 829 829 CD cord-022235-6ircruag 557 6 ) ) -RRB- cord-022235-6ircruag 557 7 found find VBD cord-022235-6ircruag 557 8 that that IN cord-022235-6ircruag 557 9 a a DT cord-022235-6ircruag 557 10 human human JJ cord-022235-6ircruag 557 11 liver liver NN cord-022235-6ircruag 557 12 cell cell NN cord-022235-6ircruag 557 13 line line NN cord-022235-6ircruag 557 14 devoid devoid JJ cord-022235-6ircruag 557 15 of of IN cord-022235-6ircruag 557 16 iV iv CD cord-022235-6ircruag 557 17 - - HYPH cord-022235-6ircruag 557 18 acetylglucosamine acetylglucosamine NN cord-022235-6ircruag 557 19 - - HYPH cord-022235-6ircruag 557 20 l l NN cord-022235-6ircruag 557 21 - - HYPH cord-022235-6ircruag 557 22 phosphotransferase phosphotransferase JJ cord-022235-6ircruag 557 23 [ [ -LRB- cord-022235-6ircruag 557 24 and and CC cord-022235-6ircruag 557 25 there there RB cord-022235-6ircruag 557 26 fore fore RB cord-022235-6ircruag 557 27 unable unable JJ cord-022235-6ircruag 557 28 to to TO cord-022235-6ircruag 557 29 phosphorylate phosphorylate VB cord-022235-6ircruag 557 30 mannose mannose NN cord-022235-6ircruag 557 31 residues residue NNS cord-022235-6ircruag 557 32 ( ( -LRB- cord-022235-6ircruag 557 33 see see VB cord-022235-6ircruag 557 34 Section section NN cord-022235-6ircruag 557 35 V.D.I.a V.D.I.a NNP cord-022235-6ircruag 557 36 ) ) -RRB- cord-022235-6ircruag 557 37 ] ] -RRB- cord-022235-6ircruag 558 1 , , , cord-022235-6ircruag 558 2 still still RB cord-022235-6ircruag 558 3 targeted target VBD cord-022235-6ircruag 558 4 some some DT cord-022235-6ircruag 558 5 lysosomal lysosomal JJ cord-022235-6ircruag 558 6 enzymes enzyme NNS cord-022235-6ircruag 558 7 correctly correctly RB cord-022235-6ircruag 558 8 with with IN cord-022235-6ircruag 558 9 , , , cord-022235-6ircruag 558 10 at at IN cord-022235-6ircruag 558 11 most most JJS cord-022235-6ircruag 558 12 , , , cord-022235-6ircruag 558 13 only only RB cord-022235-6ircruag 558 14 slightly slightly RB cord-022235-6ircruag 558 15 reduced reduce VBN cord-022235-6ircruag 558 16 efficiency efficiency NN cord-022235-6ircruag 558 17 . . . cord-022235-6ircruag 559 1 These these DT cord-022235-6ircruag 559 2 cells cell NNS cord-022235-6ircruag 559 3 may may MD cord-022235-6ircruag 559 4 have have VB cord-022235-6ircruag 559 5 a a DT cord-022235-6ircruag 559 6 completely completely RB cord-022235-6ircruag 559 7 M6P m6p JJ cord-022235-6ircruag 559 8 - - HYPH cord-022235-6ircruag 559 9 independent independent JJ cord-022235-6ircruag 559 10 system system NN cord-022235-6ircruag 559 11 for for IN cord-022235-6ircruag 559 12 sorting sort VBG cord-022235-6ircruag 559 13 lysosomal lysosomal JJ cord-022235-6ircruag 559 14 enzymes enzyme NNS cord-022235-6ircruag 559 15 . . . cord-022235-6ircruag 560 1 All all DT cord-022235-6ircruag 560 2 lysosomal lysosomal JJ cord-022235-6ircruag 560 3 membrane membrane NN cord-022235-6ircruag 560 4 proteins protein NNS cord-022235-6ircruag 560 5 are be VBP cord-022235-6ircruag 560 6 also also RB cord-022235-6ircruag 560 7 devoid devoid JJ cord-022235-6ircruag 560 8 of of IN cord-022235-6ircruag 560 9 M6P m6p NN cord-022235-6ircruag 560 10 residues residue NNS cord-022235-6ircruag 560 11 . . . cord-022235-6ircruag 561 1 Therefore therefore RB cord-022235-6ircruag 561 2 , , , cord-022235-6ircruag 561 3 some some DT cord-022235-6ircruag 561 4 lysosomal lysosomal JJ cord-022235-6ircruag 561 5 enzymes enzyme NNS cord-022235-6ircruag 561 6 may may MD cord-022235-6ircruag 561 7 have have VB cord-022235-6ircruag 561 8 membrane membrane NN cord-022235-6ircruag 561 9 - - HYPH cord-022235-6ircruag 561 10 associated associate VBN cord-022235-6ircruag 561 11 intermediates intermediate NNS cord-022235-6ircruag 561 12 which which WDT cord-022235-6ircruag 561 13 are be VBP cord-022235-6ircruag 561 14 sorted sort VBN cord-022235-6ircruag 561 15 to to IN cord-022235-6ircruag 561 16 the the DT cord-022235-6ircruag 561 17 lysosome lysosome NN cord-022235-6ircruag 561 18 together together RB cord-022235-6ircruag 561 19 with with IN cord-022235-6ircruag 561 20 authentic authentic JJ cord-022235-6ircruag 561 21 lysosomal lysosomal JJ cord-022235-6ircruag 561 22 membrane membrane NN cord-022235-6ircruag 561 23 proteins protein NNS cord-022235-6ircruag 561 24 . . . cord-022235-6ircruag 562 1 Barriocanal Barriocanal NNP cord-022235-6ircruag 562 2 et et NNP cord-022235-6ircruag 562 3 al al NNP cord-022235-6ircruag 562 4 . . . cord-022235-6ircruag 563 1 ( ( -LRB- cord-022235-6ircruag 563 2 49 49 CD cord-022235-6ircruag 563 3 ) ) -RRB- cord-022235-6ircruag 563 4 used use VBD cord-022235-6ircruag 563 5 immunocytochemistry immunocytochemistry NNP cord-022235-6ircruag 563 6 to to TO cord-022235-6ircruag 563 7 follow follow VB cord-022235-6ircruag 563 8 the the DT cord-022235-6ircruag 563 9 fate fate NN cord-022235-6ircruag 563 10 of of IN cord-022235-6ircruag 563 11 three three CD cord-022235-6ircruag 563 12 lysosomal lysosomal JJ cord-022235-6ircruag 563 13 membrane membrane NN cord-022235-6ircruag 563 14 proteins protein NNS cord-022235-6ircruag 563 15 which which WDT cord-022235-6ircruag 563 16 they -PRON- PRP cord-022235-6ircruag 563 17 detected detect VBD cord-022235-6ircruag 563 18 in in IN cord-022235-6ircruag 563 19 lysosomes lysosome NNS cord-022235-6ircruag 563 20 , , , cord-022235-6ircruag 563 21 the the DT cord-022235-6ircruag 563 22 Golgi Golgi NNP cord-022235-6ircruag 563 23 apparatus apparatus NN cord-022235-6ircruag 563 24 , , , cord-022235-6ircruag 563 25 and and CC cord-022235-6ircruag 563 26 coated coated JJ cord-022235-6ircruag 563 27 and and CC cord-022235-6ircruag 563 28 uncoated uncoated JJ cord-022235-6ircruag 563 29 vesicles vesicle NNS cord-022235-6ircruag 563 30 in in IN cord-022235-6ircruag 563 31 the the DT cord-022235-6ircruag 563 32 region region NN cord-022235-6ircruag 563 33 of of IN cord-022235-6ircruag 563 34 the the DT cord-022235-6ircruag 563 35 TGN TGN NNP cord-022235-6ircruag 563 36 . . . cord-022235-6ircruag 564 1 They -PRON- PRP cord-022235-6ircruag 564 2 found find VBD cord-022235-6ircruag 564 3 that that IN cord-022235-6ircruag 564 4 oligosaccharide oligosaccharide NN cord-022235-6ircruag 564 5 modifications modification NNS cord-022235-6ircruag 564 6 were be VBD cord-022235-6ircruag 564 7 not not RB cord-022235-6ircruag 564 8 required require VBN cord-022235-6ircruag 564 9 for for IN cord-022235-6ircruag 564 10 lysosomal lysosomal JJ cord-022235-6ircruag 564 11 targeting targeting NN cord-022235-6ircruag 564 12 ( ( -LRB- cord-022235-6ircruag 564 13 although although IN cord-022235-6ircruag 564 14 they -PRON- PRP cord-022235-6ircruag 564 15 may may MD cord-022235-6ircruag 564 16 be be VB cord-022235-6ircruag 564 17 required require VBN cord-022235-6ircruag 564 18 to to TO cord-022235-6ircruag 564 19 protect protect VB cord-022235-6ircruag 564 20 against against IN cord-022235-6ircruag 564 21 proteolysis proteolysis NN cord-022235-6ircruag 564 22 ) ) -RRB- cord-022235-6ircruag 564 23 . . . cord-022235-6ircruag 565 1 Thus thus RB cord-022235-6ircruag 565 2 the the DT cord-022235-6ircruag 565 3 sorting sorting NN cord-022235-6ircruag 565 4 pathway pathway NN cord-022235-6ircruag 565 5 for for IN cord-022235-6ircruag 565 6 these these DT cord-022235-6ircruag 565 7 proteins protein NNS cord-022235-6ircruag 565 8 remains remain VBZ cord-022235-6ircruag 565 9 to to TO cord-022235-6ircruag 565 10 be be VB cord-022235-6ircruag 565 11 determined determine VBN cord-022235-6ircruag 565 12 ; ; : cord-022235-6ircruag 565 13 they -PRON- PRP cord-022235-6ircruag 565 14 could could MD cord-022235-6ircruag 565 15 be be VB cord-022235-6ircruag 565 16 sorted sort VBN cord-022235-6ircruag 565 17 completely completely RB cord-022235-6ircruag 565 18 independently independently RB cord-022235-6ircruag 565 19 of of IN cord-022235-6ircruag 565 20 lysosomal lysosomal JJ cord-022235-6ircruag 565 21 enzymes enzyme NNS cord-022235-6ircruag 565 22 or or CC cord-022235-6ircruag 565 23 could could MD cord-022235-6ircruag 565 24 be be VB cord-022235-6ircruag 565 25 colocalized colocalize VBN cord-022235-6ircruag 565 26 to to IN cord-022235-6ircruag 565 27 the the DT cord-022235-6ircruag 565 28 same same JJ cord-022235-6ircruag 565 29 coated coated JJ cord-022235-6ircruag 565 30 vesicles vesicle NNS cord-022235-6ircruag 565 31 by by IN cord-022235-6ircruag 565 32 an an DT cord-022235-6ircruag 565 33 M6Pindependent m6pindependent JJ cord-022235-6ircruag 565 34 receptor receptor NN cord-022235-6ircruag 565 35 and and CC cord-022235-6ircruag 565 36 then then RB cord-022235-6ircruag 565 37 segregated segregate VBD cord-022235-6ircruag 565 38 from from IN cord-022235-6ircruag 565 39 recycling recycle VBG cord-022235-6ircruag 565 40 vesicle vesicle NN cord-022235-6ircruag 565 41 mem mem NNP cord-022235-6ircruag 565 42 brane brane NN cord-022235-6ircruag 565 43 components component NNS cord-022235-6ircruag 565 44 in in IN cord-022235-6ircruag 565 45 the the DT cord-022235-6ircruag 565 46 prelysosome prelysosome NN cord-022235-6ircruag 565 47 . . . cord-022235-6ircruag 566 1 Green Green NNP cord-022235-6ircruag 566 2 et et FW cord-022235-6ircruag 566 3 al al NNP cord-022235-6ircruag 566 4 . . . cord-022235-6ircruag 567 1 ( ( -LRB- cord-022235-6ircruag 567 2 397 397 LS cord-022235-6ircruag 567 3 ) ) -RRB- cord-022235-6ircruag 567 4 have have VBP cord-022235-6ircruag 567 5 recently recently RB cord-022235-6ircruag 567 6 found find VBN cord-022235-6ircruag 567 7 that that IN cord-022235-6ircruag 567 8 newly newly RB cord-022235-6ircruag 567 9 synthesized synthesize VBN cord-022235-6ircruag 567 10 lysosomal lysosomal JJ cord-022235-6ircruag 567 11 membrane membrane NN cord-022235-6ircruag 567 12 proteins protein NNS cord-022235-6ircruag 567 13 appear appear VBP cord-022235-6ircruag 567 14 in in IN cord-022235-6ircruag 567 15 lysosomes lysosome NNS cord-022235-6ircruag 567 16 with with IN cord-022235-6ircruag 567 17 the the DT cord-022235-6ircruag 567 18 same same JJ cord-022235-6ircruag 567 19 kinetics kinetic NNS cord-022235-6ircruag 567 20 as as IN cord-022235-6ircruag 567 21 newly newly RB cord-022235-6ircruag 567 22 synthesized synthesize VBN cord-022235-6ircruag 567 23 plasma plasma NN cord-022235-6ircruag 567 24 membrane membrane NN cord-022235-6ircruag 567 25 proteins protein NNS cord-022235-6ircruag 567 26 appear appear VBP cord-022235-6ircruag 567 27 at at IN cord-022235-6ircruag 567 28 the the DT cord-022235-6ircruag 567 29 cell cell NN cord-022235-6ircruag 567 30 surface surface NN cord-022235-6ircruag 567 31 , , , cord-022235-6ircruag 567 32 making make VBG cord-022235-6ircruag 567 33 it -PRON- PRP cord-022235-6ircruag 567 34 unlikely unlikely JJ cord-022235-6ircruag 567 35 that that IN cord-022235-6ircruag 567 36 the the DT cord-022235-6ircruag 567 37 former former JJ cord-022235-6ircruag 567 38 pass pass NN cord-022235-6ircruag 567 39 via via IN cord-022235-6ircruag 567 40 the the DT cord-022235-6ircruag 567 41 plasma plasma NN cord-022235-6ircruag 567 42 membrane membrane NN cord-022235-6ircruag 567 43 en en IN cord-022235-6ircruag 567 44 route route NN cord-022235-6ircruag 567 45 to to IN cord-022235-6ircruag 567 46 the the DT cord-022235-6ircruag 567 47 lysosome lysosome NN cord-022235-6ircruag 567 48 . . . cord-022235-6ircruag 568 1 Although although IN cord-022235-6ircruag 568 2 vacuoles vacuole NNS cord-022235-6ircruag 568 3 are be VBP cord-022235-6ircruag 568 4 the the DT cord-022235-6ircruag 568 5 functional functional JJ cord-022235-6ircruag 568 6 equivalents equivalent NNS cord-022235-6ircruag 568 7 of of IN cord-022235-6ircruag 568 8 lysosomes lysosome NNS cord-022235-6ircruag 568 9 in in IN cord-022235-6ircruag 568 10 animal animal NN cord-022235-6ircruag 568 11 cells cell NNS cord-022235-6ircruag 568 12 , , , cord-022235-6ircruag 568 13 the the DT cord-022235-6ircruag 568 14 sorting sorting NN cord-022235-6ircruag 568 15 of of IN cord-022235-6ircruag 568 16 vacuolar vacuolar JJ cord-022235-6ircruag 568 17 enzymes enzyme NNS cord-022235-6ircruag 568 18 is be VBZ cord-022235-6ircruag 568 19 completely completely RB cord-022235-6ircruag 568 20 independent independent JJ cord-022235-6ircruag 568 21 of of IN cord-022235-6ircruag 568 22 M6P M6P NNP cord-022235-6ircruag 568 23 receptors receptor NNS cord-022235-6ircruag 568 24 . . . cord-022235-6ircruag 569 1 This this DT cord-022235-6ircruag 569 2 was be VBD cord-022235-6ircruag 569 3 demonstrated demonstrate VBN cord-022235-6ircruag 569 4 most most RBS cord-022235-6ircruag 569 5 simply simply RB cord-022235-6ircruag 569 6 by by IN cord-022235-6ircruag 569 7 the the DT cord-022235-6ircruag 569 8 fact fact NN cord-022235-6ircruag 569 9 that that IN cord-022235-6ircruag 569 10 tunicamy tunicamy NNP cord-022235-6ircruag 569 11 cin cin NNP cord-022235-6ircruag 569 12 treatment treatment NN cord-022235-6ircruag 569 13 did do VBD cord-022235-6ircruag 569 14 not not RB cord-022235-6ircruag 569 15 affect affect VB cord-022235-6ircruag 569 16 vacuolar vacuolar NNP cord-022235-6ircruag 569 17 protein protein NN cord-022235-6ircruag 569 18 targeting target VBG cord-022235-6ircruag 569 19 ( ( -LRB- cord-022235-6ircruag 569 20 572,1025 572,1025 NNP cord-022235-6ircruag 569 21 ) ) -RRB- cord-022235-6ircruag 569 22 . . . cord-022235-6ircruag 570 1 How how WRB cord-022235-6ircruag 570 2 ever ever RB cord-022235-6ircruag 570 3 , , , cord-022235-6ircruag 570 4 at at RB cord-022235-6ircruag 570 5 least least RBS cord-022235-6ircruag 570 6 one one CD cord-022235-6ircruag 570 7 vacuolar vacuolar NNP cord-022235-6ircruag 570 8 protein protein NN cord-022235-6ircruag 570 9 does do VBZ cord-022235-6ircruag 570 10 have have VB cord-022235-6ircruag 570 11 phosphorylated phosphorylate VBN cord-022235-6ircruag 570 12 mannose mannose JJ cord-022235-6ircruag 570 13 residues residue NNS cord-022235-6ircruag 570 14 . . . cord-022235-6ircruag 571 1 Most Most JJS cord-022235-6ircruag 571 2 of of IN cord-022235-6ircruag 571 3 the the DT cord-022235-6ircruag 571 4 work work NN cord-022235-6ircruag 571 5 on on IN cord-022235-6ircruag 571 6 the the DT cord-022235-6ircruag 571 7 sorting sorting NN cord-022235-6ircruag 571 8 of of IN cord-022235-6ircruag 571 9 vacuolar vacuolar NNP cord-022235-6ircruag 571 10 proteins protein NNS cord-022235-6ircruag 571 11 has have VBZ cord-022235-6ircruag 571 12 concentrated concentrate VBN cord-022235-6ircruag 571 13 on on IN cord-022235-6ircruag 571 14 the the DT cord-022235-6ircruag 571 15 identification identification NN cord-022235-6ircruag 571 16 of of IN cord-022235-6ircruag 571 17 sorting sort VBG cord-022235-6ircruag 571 18 signals signal NNS cord-022235-6ircruag 571 19 in in IN cord-022235-6ircruag 571 20 yeast yeast NN cord-022235-6ircruag 571 21 vacuolar vacuolar NNP cord-022235-6ircruag 571 22 proteins protein NNS cord-022235-6ircruag 571 23 . . . cord-022235-6ircruag 572 1 Early early JJ cord-022235-6ircruag 572 2 studies study NNS cord-022235-6ircruag 572 3 showed show VBD cord-022235-6ircruag 572 4 that that IN cord-022235-6ircruag 572 5 vacuolar vacuolar NNP cord-022235-6ircruag 572 6 proteases protease NNS cord-022235-6ircruag 572 7 were be VBD cord-022235-6ircruag 572 8 proteolytically proteolytically RB cord-022235-6ircruag 572 9 processed process VBN cord-022235-6ircruag 572 10 in in IN cord-022235-6ircruag 572 11 two two CD cord-022235-6ircruag 572 12 distinct distinct JJ cord-022235-6ircruag 572 13 stages stage NNS cord-022235-6ircruag 572 14 , , , cord-022235-6ircruag 572 15 the the DT cord-022235-6ircruag 572 16 first first JJ cord-022235-6ircruag 572 17 of of IN cord-022235-6ircruag 572 18 which which WDT cord-022235-6ircruag 572 19 corresponded correspond VBD cord-022235-6ircruag 572 20 to to IN cord-022235-6ircruag 572 21 the the DT cord-022235-6ircruag 572 22 removal removal NN cord-022235-6ircruag 572 23 of of IN cord-022235-6ircruag 572 24 a a DT cord-022235-6ircruag 572 25 signal signal JJ cord-022235-6ircruag 572 26 peptide peptide NN cord-022235-6ircruag 572 27 ( ( -LRB- cord-022235-6ircruag 572 28 711 711 CD cord-022235-6ircruag 572 29 ) ) -RRB- cord-022235-6ircruag 572 30 . . . cord-022235-6ircruag 573 1 The the DT cord-022235-6ircruag 573 2 second second JJ cord-022235-6ircruag 573 3 processing processing NN cord-022235-6ircruag 573 4 step step NN cord-022235-6ircruag 573 5 is be VBZ cord-022235-6ircruag 573 6 catalyzed catalyze VBN cord-022235-6ircruag 573 7 by by IN cord-022235-6ircruag 573 8 a a DT cord-022235-6ircruag 573 9 vacuo vacuo NN cord-022235-6ircruag 573 10 lar lar NN cord-022235-6ircruag 573 11 protease protease NN cord-022235-6ircruag 573 12 , , , cord-022235-6ircruag 573 13 proteinase proteinase NN cord-022235-6ircruag 573 14 A a NN cord-022235-6ircruag 573 15 , , , cord-022235-6ircruag 573 16 which which WDT cord-022235-6ircruag 573 17 removes remove VBZ cord-022235-6ircruag 573 18 an an DT cord-022235-6ircruag 573 19 additional additional JJ cord-022235-6ircruag 573 20 N n CD cord-022235-6ircruag 573 21 - - HYPH cord-022235-6ircruag 573 22 terminal terminal JJ cord-022235-6ircruag 573 23 poly poly JJ cord-022235-6ircruag 573 24 peptide peptide NN cord-022235-6ircruag 573 25 segment segment NN cord-022235-6ircruag 573 26 , , , cord-022235-6ircruag 573 27 the the DT cord-022235-6ircruag 573 28 propeptide propeptide NN cord-022235-6ircruag 573 29 , , , cord-022235-6ircruag 573 30 from from IN cord-022235-6ircruag 573 31 other other JJ cord-022235-6ircruag 573 32 vacuolar vacuolar JJ cord-022235-6ircruag 573 33 enzymes enzyme NNS cord-022235-6ircruag 573 34 . . . cord-022235-6ircruag 574 1 Protein protein NN cord-022235-6ircruag 574 2 ase ase NNP cord-022235-6ircruag 574 3 A A NNP cord-022235-6ircruag 574 4 is be VBZ cord-022235-6ircruag 574 5 also also RB cord-022235-6ircruag 574 6 autoactivated autoactivate VBN cord-022235-6ircruag 574 7 by by IN cord-022235-6ircruag 574 8 the the DT cord-022235-6ircruag 574 9 same same JJ cord-022235-6ircruag 574 10 mechanism mechanism NN cord-022235-6ircruag 574 11 ( ( -LRB- cord-022235-6ircruag 574 12 15,1206 15,1206 CD cord-022235-6ircruag 574 13 ) ) -RRB- cord-022235-6ircruag 574 14 . . . cord-022235-6ircruag 575 1 The the DT cord-022235-6ircruag 575 2 second second JJ cord-022235-6ircruag 575 3 processing processing NN cord-022235-6ircruag 575 4 step step NN cord-022235-6ircruag 575 5 is be VBZ cord-022235-6ircruag 575 6 blocked block VBN cord-022235-6ircruag 575 7 by by IN cord-022235-6ircruag 575 8 certain certain JJ cord-022235-6ircruag 575 9 sec sec NNP cord-022235-6ircruag 575 10 mutations mutation NNS cord-022235-6ircruag 575 11 , , , cord-022235-6ircruag 575 12 which which WDT cord-022235-6ircruag 575 13 cause cause VBP cord-022235-6ircruag 575 14 secre secre NNP cord-022235-6ircruag 575 15 tory tory NNP cord-022235-6ircruag 575 16 proteins protein NNS cord-022235-6ircruag 575 17 to to TO cord-022235-6ircruag 575 18 accumulate accumulate VB cord-022235-6ircruag 575 19 in in IN cord-022235-6ircruag 575 20 the the DT cord-022235-6ircruag 575 21 RER RER NNP cord-022235-6ircruag 575 22 or or CC cord-022235-6ircruag 575 23 Golgi Golgi NNP cord-022235-6ircruag 575 24 apparatus apparatus NN cord-022235-6ircruag 575 25 , , , cord-022235-6ircruag 575 26 whereas whereas IN cord-022235-6ircruag 575 27 mutations mutation NNS cord-022235-6ircruag 575 28 which which WDT cord-022235-6ircruag 575 29 affect affect VBP cord-022235-6ircruag 575 30 the the DT cord-022235-6ircruag 575 31 final final JJ cord-022235-6ircruag 575 32 stage stage NN cord-022235-6ircruag 575 33 of of IN cord-022235-6ircruag 575 34 the the DT cord-022235-6ircruag 575 35 secretory secretory JJ cord-022235-6ircruag 575 36 pathway pathway NN cord-022235-6ircruag 575 37 from from IN cord-022235-6ircruag 575 38 the the DT cord-022235-6ircruag 575 39 Golgi Golgi NNP cord-022235-6ircruag 575 40 to to IN cord-022235-6ircruag 575 41 the the DT cord-022235-6ircruag 575 42 cell cell NN cord-022235-6ircruag 575 43 surface surface NN cord-022235-6ircruag 575 44 do do VBP cord-022235-6ircruag 575 45 not not RB cord-022235-6ircruag 575 46 affect affect VB cord-022235-6ircruag 575 47 vacuolar vacuolar NNP cord-022235-6ircruag 575 48 protein protein NN cord-022235-6ircruag 575 49 sorting sorting NN cord-022235-6ircruag 575 50 or or CC cord-022235-6ircruag 575 51 process process NN cord-022235-6ircruag 575 52 ing ing NN cord-022235-6ircruag 575 53 ( ( -LRB- cord-022235-6ircruag 575 54 1082 1082 CD cord-022235-6ircruag 575 55 ) ) -RRB- cord-022235-6ircruag 575 56 . . . cord-022235-6ircruag 576 1 This this DT cord-022235-6ircruag 576 2 implies imply VBZ cord-022235-6ircruag 576 3 that that IN cord-022235-6ircruag 576 4 the the DT cord-022235-6ircruag 576 5 Golgi Golgi NNP cord-022235-6ircruag 576 6 is be VBZ cord-022235-6ircruag 576 7 the the DT cord-022235-6ircruag 576 8 site site NN cord-022235-6ircruag 576 9 of of IN cord-022235-6ircruag 576 10 sorting sorting NN cord-022235-6ircruag 576 11 of of IN cord-022235-6ircruag 576 12 vacuolar vacuolar NN cord-022235-6ircruag 576 13 and and CC cord-022235-6ircruag 576 14 secreted secrete VBN cord-022235-6ircruag 576 15 or or CC cord-022235-6ircruag 576 16 plasma plasma NN cord-022235-6ircruag 576 17 membrane membrane NN cord-022235-6ircruag 576 18 proteins protein NNS cord-022235-6ircruag 576 19 in in IN cord-022235-6ircruag 576 20 yeast yeast NN cord-022235-6ircruag 576 21 cells cell NNS cord-022235-6ircruag 576 22 . . . cord-022235-6ircruag 577 1 Gene gene NN cord-022235-6ircruag 577 2 fusion fusion NN cord-022235-6ircruag 577 3 studies study NNS cord-022235-6ircruag 577 4 conclusively conclusively RB cord-022235-6ircruag 577 5 demonstrated demonstrate VBD cord-022235-6ircruag 577 6 that that IN cord-022235-6ircruag 577 7 propeptides propeptides NNP cord-022235-6ircruag 577 8 are be VBP cord-022235-6ircruag 577 9 vacuolar vacuolar NN cord-022235-6ircruag 577 10 sorting sorting NN cord-022235-6ircruag 577 11 signals signal NNS cord-022235-6ircruag 577 12 . . . cord-022235-6ircruag 578 1 Bankaitis Bankaitis NNP cord-022235-6ircruag 578 2 et et NNP cord-022235-6ircruag 578 3 al al NNP cord-022235-6ircruag 578 4 . . . cord-022235-6ircruag 579 1 ( ( -LRB- cord-022235-6ircruag 579 2 44 44 CD cord-022235-6ircruag 579 3 ) ) -RRB- cord-022235-6ircruag 579 4 and and CC cord-022235-6ircruag 579 5 Johnson Johnson NNP cord-022235-6ircruag 579 6 et et NNP cord-022235-6ircruag 579 7 al al NNP cord-022235-6ircruag 579 8 . . . cord-022235-6ircruag 580 1 ( ( -LRB- cord-022235-6ircruag 580 2 541 541 CD cord-022235-6ircruag 580 3 ) ) -RRB- cord-022235-6ircruag 580 4 found find VBD cord-022235-6ircruag 580 5 that that IN cord-022235-6ircruag 580 6 at at RB cord-022235-6ircruag 580 7 most most JJS cord-022235-6ircruag 580 8 50 50 CD cord-022235-6ircruag 580 9 N n CD cord-022235-6ircruag 580 10 - - HYPH cord-022235-6ircruag 580 11 terminal terminal NN cord-022235-6ircruag 580 12 residues residue NNS cord-022235-6ircruag 580 13 of of IN cord-022235-6ircruag 580 14 preprocarboxypeptidase preprocarboxypeptidase NNP cord-022235-6ircruag 580 15 Y Y NNP cord-022235-6ircruag 580 16 ( ( -LRB- cord-022235-6ircruag 580 17 CPY CPY NNP cord-022235-6ircruag 580 18 ) ) -RRB- cord-022235-6ircruag 580 19 , , , cord-022235-6ircruag 580 20 including include VBG cord-022235-6ircruag 580 21 the the DT cord-022235-6ircruag 580 22 20-residue 20-residue CD cord-022235-6ircruag 580 23 propeptide propeptide NN cord-022235-6ircruag 580 24 , , , cord-022235-6ircruag 580 25 could could MD cord-022235-6ircruag 580 26 target target VB cord-022235-6ircruag 580 27 the the DT cord-022235-6ircruag 580 28 normally normally RB cord-022235-6ircruag 580 29 secreted secrete VBN cord-022235-6ircruag 580 30 enzyme enzyme NN cord-022235-6ircruag 580 31 invertase invertase NN cord-022235-6ircruag 580 32 into into IN cord-022235-6ircruag 580 33 the the DT cord-022235-6ircruag 580 34 vacuole vacuole NN cord-022235-6ircruag 580 35 . . . cord-022235-6ircruag 581 1 Similar similar JJ cord-022235-6ircruag 581 2 results result NNS cord-022235-6ircruag 581 3 were be VBD cord-022235-6ircruag 581 4 obtained obtain VBN cord-022235-6ircruag 581 5 with with IN cord-022235-6ircruag 581 6 proteinase proteinase NN cord-022235-6ircruag 581 7 A A NNP cord-022235-6ircruag 581 8 - - HYPH cord-022235-6ircruag 581 9 invertase invertase NN cord-022235-6ircruag 581 10 hybrids hybrid NNS cord-022235-6ircruag 581 11 ( ( -LRB- cord-022235-6ircruag 581 12 572 572 CD cord-022235-6ircruag 581 13 ) ) -RRB- cord-022235-6ircruag 581 14 . . . cord-022235-6ircruag 582 1 Vails Vails NNP cord-022235-6ircruag 582 2 et et NNP cord-022235-6ircruag 582 3 al al NNP cord-022235-6ircruag 582 4 . . . cord-022235-6ircruag 583 1 ( ( -LRB- cord-022235-6ircruag 583 2 1137 1137 CD cord-022235-6ircruag 583 3 ) ) -RRB- cord-022235-6ircruag 583 4 subse subse NNP cord-022235-6ircruag 583 5 quently quently RB cord-022235-6ircruag 583 6 found find VBD cord-022235-6ircruag 583 7 that that IN cord-022235-6ircruag 583 8 mutations mutation NNS cord-022235-6ircruag 583 9 affecting affect VBG cord-022235-6ircruag 583 10 the the DT cord-022235-6ircruag 583 11 sequence sequence NN cord-022235-6ircruag 583 12 of of IN cord-022235-6ircruag 583 13 the the DT cord-022235-6ircruag 583 14 proCPY procpy JJ cord-022235-6ircruag 583 15 pro pro JJ cord-022235-6ircruag 583 16 peptide peptide NN cord-022235-6ircruag 583 17 caused cause VBD cord-022235-6ircruag 583 18 the the DT cord-022235-6ircruag 583 19 enzyme enzyme NN cord-022235-6ircruag 583 20 to to TO cord-022235-6ircruag 583 21 be be VB cord-022235-6ircruag 583 22 secreted secrete VBN cord-022235-6ircruag 583 23 in in IN cord-022235-6ircruag 583 24 an an DT cord-022235-6ircruag 583 25 inactive inactive JJ cord-022235-6ircruag 583 26 form form NN cord-022235-6ircruag 583 27 . . . cord-022235-6ircruag 584 1 There there EX cord-022235-6ircruag 584 2 appears appear VBZ cord-022235-6ircruag 584 3 to to TO cord-022235-6ircruag 584 4 be be VB cord-022235-6ircruag 584 5 no no DT cord-022235-6ircruag 584 6 sequence sequence NN cord-022235-6ircruag 584 7 similarity similarity NN cord-022235-6ircruag 584 8 between between IN cord-022235-6ircruag 584 9 the the DT cord-022235-6ircruag 584 10 propeptides propeptide NNS cord-022235-6ircruag 584 11 of of IN cord-022235-6ircruag 584 12 different different JJ cord-022235-6ircruag 584 13 yeast yeast NN cord-022235-6ircruag 584 14 vacuolar vacuolar NN cord-022235-6ircruag 584 15 enzymes enzyme NNS cord-022235-6ircruag 584 16 , , , cord-022235-6ircruag 584 17 even even RB cord-022235-6ircruag 584 18 though though IN cord-022235-6ircruag 584 19 genetic genetic JJ cord-022235-6ircruag 584 20 studies study NNS cord-022235-6ircruag 584 21 described describe VBN cord-022235-6ircruag 584 22 below below RB cord-022235-6ircruag 584 23 suggest suggest VBP cord-022235-6ircruag 584 24 that that IN cord-022235-6ircruag 584 25 they -PRON- PRP cord-022235-6ircruag 584 26 are be VBP cord-022235-6ircruag 584 27 sorted sort VBN cord-022235-6ircruag 584 28 into into IN cord-022235-6ircruag 584 29 the the DT cord-022235-6ircruag 584 30 vacuole vacuole NN cord-022235-6ircruag 584 31 via via IN cord-022235-6ircruag 584 32 a a DT cord-022235-6ircruag 584 33 common common JJ cord-022235-6ircruag 584 34 pathway pathway NN cord-022235-6ircruag 584 35 . . . cord-022235-6ircruag 585 1 Part part NN cord-022235-6ircruag 585 2 of of IN cord-022235-6ircruag 585 3 the the DT cord-022235-6ircruag 585 4 propeptide propeptide NN cord-022235-6ircruag 585 5 may may MD cord-022235-6ircruag 585 6 be be VB cord-022235-6ircruag 585 7 required require VBN cord-022235-6ircruag 585 8 to to TO cord-022235-6ircruag 585 9 maintain maintain VB cord-022235-6ircruag 585 10 vacuolar vacuolar JJ cord-022235-6ircruag 585 11 enzymes enzyme NNS cord-022235-6ircruag 585 12 in in IN cord-022235-6ircruag 585 13 an an DT cord-022235-6ircruag 585 14 inactive inactive JJ cord-022235-6ircruag 585 15 form form NN cord-022235-6ircruag 585 16 until until IN cord-022235-6ircruag 585 17 they -PRON- PRP cord-022235-6ircruag 585 18 reach reach VBP cord-022235-6ircruag 585 19 the the DT cord-022235-6ircruag 585 20 vacuole vacuole NN cord-022235-6ircruag 585 21 and and CC cord-022235-6ircruag 585 22 may may MD cord-022235-6ircruag 585 23 also also RB cord-022235-6ircruag 585 24 maintain maintain VB cord-022235-6ircruag 585 25 the the DT cord-022235-6ircruag 585 26 precursors precursor NNS cord-022235-6ircruag 585 27 in in IN cord-022235-6ircruag 585 28 a a DT cord-022235-6ircruag 585 29 competent competent JJ cord-022235-6ircruag 585 30 conformation conformation NN cord-022235-6ircruag 585 31 for for IN cord-022235-6ircruag 585 32 transport transport NN cord-022235-6ircruag 585 33 through through IN cord-022235-6ircruag 585 34 the the DT cord-022235-6ircruag 585 35 secre secre NNP cord-022235-6ircruag 585 36 tory tory NNP cord-022235-6ircruag 585 37 pathway pathway NNP cord-022235-6ircruag 585 38 . . . cord-022235-6ircruag 586 1 The the DT cord-022235-6ircruag 586 2 overproduction overproduction NN cord-022235-6ircruag 586 3 of of IN cord-022235-6ircruag 586 4 vacuolar vacuolar NNP cord-022235-6ircruag 586 5 proteinase proteinase NNP cord-022235-6ircruag 586 6 A A NNP cord-022235-6ircruag 586 7 ( ( -LRB- cord-022235-6ircruag 586 8 972 972 CD cord-022235-6ircruag 586 9 ) ) -RRB- cord-022235-6ircruag 586 10 and and CC cord-022235-6ircruag 586 11 of of IN cord-022235-6ircruag 586 12 CPY CPY NNP cord-022235-6ircruag 586 13 - - HYPH cord-022235-6ircruag 586 14 inver inver NNP cord-022235-6ircruag 586 15 - - HYPH cord-022235-6ircruag 586 16 tase tase NNP cord-022235-6ircruag 586 17 hybrids hybrid NNS cord-022235-6ircruag 586 18 ( ( -LRB- cord-022235-6ircruag 586 19 44 44 CD cord-022235-6ircruag 586 20 ) ) -RRB- cord-022235-6ircruag 586 21 causes cause VBZ cord-022235-6ircruag 586 22 them -PRON- PRP cord-022235-6ircruag 586 23 to to TO cord-022235-6ircruag 586 24 be be VB cord-022235-6ircruag 586 25 secreted secrete VBN cord-022235-6ircruag 586 26 into into IN cord-022235-6ircruag 586 27 the the DT cord-022235-6ircruag 586 28 medium medium NN cord-022235-6ircruag 586 29 , , , cord-022235-6ircruag 586 30 suggesting suggest VBG cord-022235-6ircruag 586 31 that that IN cord-022235-6ircruag 586 32 some some DT cord-022235-6ircruag 586 33 component component NN cord-022235-6ircruag 586 34 of of IN cord-022235-6ircruag 586 35 the the DT cord-022235-6ircruag 586 36 vacuolar vacuolar NNP cord-022235-6ircruag 586 37 sorting sort VBG cord-022235-6ircruag 586 38 pathway pathway NN cord-022235-6ircruag 586 39 ( ( -LRB- cord-022235-6ircruag 586 40 e.g. e.g. RB cord-022235-6ircruag 586 41 , , , cord-022235-6ircruag 586 42 a a DT cord-022235-6ircruag 586 43 receptor receptor NN cord-022235-6ircruag 586 44 ) ) -RRB- cord-022235-6ircruag 586 45 had have VBD cord-022235-6ircruag 586 46 been be VBN cord-022235-6ircruag 586 47 saturated saturate VBN cord-022235-6ircruag 586 48 . . . cord-022235-6ircruag 587 1 These these DT cord-022235-6ircruag 587 2 observations observation NNS cord-022235-6ircruag 587 3 led lead VBD cord-022235-6ircruag 587 4 to to IN cord-022235-6ircruag 587 5 the the DT cord-022235-6ircruag 587 6 development development NN cord-022235-6ircruag 587 7 of of IN cord-022235-6ircruag 587 8 tech tech NN cord-022235-6ircruag 587 9 niques nique NNS cord-022235-6ircruag 587 10 for for IN cord-022235-6ircruag 587 11 selecting select VBG cord-022235-6ircruag 587 12 mutants mutant NNS cord-022235-6ircruag 587 13 which which WDT cord-022235-6ircruag 587 14 secreted secrete VBD cord-022235-6ircruag 587 15 CPY cpy NN cord-022235-6ircruag 587 16 or or CC cord-022235-6ircruag 587 17 CPY cpy NN cord-022235-6ircruag 587 18 - - HYPH cord-022235-6ircruag 587 19 invertase invertase NN cord-022235-6ircruag 587 20 with with IN cord-022235-6ircruag 587 21 out out RP cord-022235-6ircruag 587 22 overproduction overproduction NN cord-022235-6ircruag 587 23 ( ( -LRB- cord-022235-6ircruag 587 24 Section section NN cord-022235-6ircruag 587 25 II.F.l.b II.F.l.b NNS cord-022235-6ircruag 587 26 ) ) -RRB- cord-022235-6ircruag 587 27 . . . cord-022235-6ircruag 588 1 Mutations mutation NNS cord-022235-6ircruag 588 2 in in IN cord-022235-6ircruag 588 3 over over IN cord-022235-6ircruag 588 4 50 50 CD cord-022235-6ircruag 588 5 different different JJ cord-022235-6ircruag 588 6 genes gene NNS cord-022235-6ircruag 588 7 ( ( -LRB- cord-022235-6ircruag 588 8 called call VBN cord-022235-6ircruag 588 9 VPL VPL NNP cord-022235-6ircruag 588 10 or or CC cord-022235-6ircruag 588 11 VPT VPT NNP cord-022235-6ircruag 588 12 ) ) -RRB- cord-022235-6ircruag 588 13 have have VBP cord-022235-6ircruag 588 14 been be VBN cord-022235-6ircruag 588 15 identified identify VBN cord-022235-6ircruag 588 16 ( ( -LRB- cord-022235-6ircruag 588 17 44,939a,971 44,939a,971 CD cord-022235-6ircruag 588 18 ) ) -RRB- cord-022235-6ircruag 588 19 . . . cord-022235-6ircruag 589 1 The the DT cord-022235-6ircruag 589 2 extent extent NN cord-022235-6ircruag 589 3 of of IN cord-022235-6ircruag 589 4 the the DT cord-022235-6ircruag 589 5 sorting sorting NN cord-022235-6ircruag 589 6 defect defect NN cord-022235-6ircruag 589 7 varied vary VBN cord-022235-6ircruag 589 8 in in IN cord-022235-6ircruag 589 9 different different JJ cord-022235-6ircruag 589 10 mutants mutant NNS cord-022235-6ircruag 589 11 : : : cord-022235-6ircruag 589 12 Some some DT cord-022235-6ircruag 589 13 of of IN cord-022235-6ircruag 589 14 them -PRON- PRP cord-022235-6ircruag 589 15 , , , cord-022235-6ircruag 589 16 for for IN cord-022235-6ircruag 589 17 exam exam NN cord-022235-6ircruag 589 18 ple ple NNS cord-022235-6ircruag 589 19 , , , cord-022235-6ircruag 589 20 did do VBD cord-022235-6ircruag 589 21 not not RB cord-022235-6ircruag 589 22 affect affect VB cord-022235-6ircruag 589 23 the the DT cord-022235-6ircruag 589 24 targeting targeting NN cord-022235-6ircruag 589 25 of of IN cord-022235-6ircruag 589 26 proteinase proteinase NN cord-022235-6ircruag 589 27 A a NN cord-022235-6ircruag 589 28 , , , cord-022235-6ircruag 589 29 and and CC cord-022235-6ircruag 589 30 none none NN cord-022235-6ircruag 589 31 of of IN cord-022235-6ircruag 589 32 them -PRON- PRP cord-022235-6ircruag 589 33 affected affect VBD cord-022235-6ircruag 589 34 the the DT cord-022235-6ircruag 589 35 sorting sorting NN cord-022235-6ircruag 589 36 of of IN cord-022235-6ircruag 589 37 the the DT cord-022235-6ircruag 589 38 vacuolar vacuolar NNP cord-022235-6ircruag 589 39 membrane membrane NN cord-022235-6ircruag 589 40 protein protein NN cord-022235-6ircruag 589 41 α α NNP cord-022235-6ircruag 589 42 - - HYPH cord-022235-6ircruag 589 43 mannosidase mannosidase NNP cord-022235-6ircruag 589 44 , , , cord-022235-6ircruag 589 45 which which WDT cord-022235-6ircruag 589 46 is be VBZ cord-022235-6ircruag 589 47 presumably presumably RB cord-022235-6ircruag 589 48 sorted sort VBN cord-022235-6ircruag 589 49 to to IN cord-022235-6ircruag 589 50 the the DT cord-022235-6ircruag 589 51 vacuole vacuole NN cord-022235-6ircruag 589 52 by by IN cord-022235-6ircruag 589 53 an an DT cord-022235-6ircruag 589 54 alternate alternate JJ cord-022235-6ircruag 589 55 pathway pathway NN cord-022235-6ircruag 589 56 ( ( -LRB- cord-022235-6ircruag 589 57 971 971 CD cord-022235-6ircruag 589 58 ) ) -RRB- cord-022235-6ircruag 589 59 . . . cord-022235-6ircruag 590 1 It -PRON- PRP cord-022235-6ircruag 590 2 is be VBZ cord-022235-6ircruag 590 3 unlikely unlikely JJ cord-022235-6ircruag 590 4 that that IN cord-022235-6ircruag 590 5 any any DT cord-022235-6ircruag 590 6 of of IN cord-022235-6ircruag 590 7 the the DT cord-022235-6ircruag 590 8 mutations mutation NNS cord-022235-6ircruag 590 9 affected affect VBD cord-022235-6ircruag 590 10 protein protein NN cord-022235-6ircruag 590 11 retention retention NN cord-022235-6ircruag 590 12 in in IN cord-022235-6ircruag 590 13 the the DT cord-022235-6ircruag 590 14 vacuole vacuole NN cord-022235-6ircruag 590 15 because because IN cord-022235-6ircruag 590 16 vacuolar vacuolar NNP cord-022235-6ircruag 590 17 enzymes enzyme NNS cord-022235-6ircruag 590 18 were be VBD cord-022235-6ircruag 590 19 not not RB cord-022235-6ircruag 590 20 terminally terminally RB cord-022235-6ircruag 590 21 processed process VBN cord-022235-6ircruag 590 22 , , , cord-022235-6ircruag 590 23 and and CC cord-022235-6ircruag 590 24 the the DT cord-022235-6ircruag 590 25 kinet kinet NNP cord-022235-6ircruag 590 26 ics ics NNP cord-022235-6ircruag 590 27 of of IN cord-022235-6ircruag 590 28 CPY CPY NNP cord-022235-6ircruag 590 29 secretion secretion NN cord-022235-6ircruag 590 30 were be VBD cord-022235-6ircruag 590 31 comparable comparable JJ cord-022235-6ircruag 590 32 to to IN cord-022235-6ircruag 590 33 those those DT cord-022235-6ircruag 590 34 of of IN cord-022235-6ircruag 590 35 a a DT cord-022235-6ircruag 590 36 normally normally RB cord-022235-6ircruag 590 37 secreted secrete VBN cord-022235-6ircruag 590 38 protein protein NN cord-022235-6ircruag 590 39 . . . cord-022235-6ircruag 591 1 The the DT cord-022235-6ircruag 591 2 characterization characterization NN cord-022235-6ircruag 591 3 of of IN cord-022235-6ircruag 591 4 the the DT cord-022235-6ircruag 591 5 VPT VPT NNP cord-022235-6ircruag 591 6 or or CC cord-022235-6ircruag 591 7 VPL VPL NNP cord-022235-6ircruag 591 8 gene gene NN cord-022235-6ircruag 591 9 products product NNS cord-022235-6ircruag 591 10 and and CC cord-022235-6ircruag 591 11 their -PRON- PRP$ cord-022235-6ircruag 591 12 localization localization NN cord-022235-6ircruag 591 13 in in IN cord-022235-6ircruag 591 14 the the DT cord-022235-6ircruag 591 15 cell cell NN cord-022235-6ircruag 591 16 could could MD cord-022235-6ircruag 591 17 provide provide VB cord-022235-6ircruag 591 18 revealing reveal VBG cord-022235-6ircruag 591 19 insights insight NNS cord-022235-6ircruag 591 20 into into IN cord-022235-6ircruag 591 21 the the DT cord-022235-6ircruag 591 22 mecha mecha JJ cord-022235-6ircruag 591 23 nisms nism NNS cord-022235-6ircruag 591 24 of of IN cord-022235-6ircruag 591 25 vacuolar vacuolar NNP cord-022235-6ircruag 591 26 protein protein NNP cord-022235-6ircruag 591 27 targeting target VBG cord-022235-6ircruag 591 28 , , , cord-022235-6ircruag 591 29 but but CC cord-022235-6ircruag 591 30 at at IN cord-022235-6ircruag 591 31 present present NN cord-022235-6ircruag 591 32 we -PRON- PRP cord-022235-6ircruag 591 33 can can MD cord-022235-6ircruag 591 34 only only RB cord-022235-6ircruag 591 35 speculate speculate VB cord-022235-6ircruag 591 36 on on IN cord-022235-6ircruag 591 37 their -PRON- PRP$ cord-022235-6ircruag 591 38 roles role NNS cord-022235-6ircruag 591 39 . . . cord-022235-6ircruag 592 1 Obvious obvious JJ cord-022235-6ircruag 592 2 candidates candidate NNS cord-022235-6ircruag 592 3 are be VBP cord-022235-6ircruag 592 4 the the DT cord-022235-6ircruag 592 5 propeptide propeptide JJ cord-022235-6ircruag 592 6 receptor receptor NN cord-022235-6ircruag 592 7 , , , cord-022235-6ircruag 592 8 vacuolar vacuolar NN cord-022235-6ircruag 592 9 or or CC cord-022235-6ircruag 592 10 Golgi Golgi NNP cord-022235-6ircruag 592 11 ATPases atpase NNS cord-022235-6ircruag 592 12 which which WDT cord-022235-6ircruag 592 13 might may MD cord-022235-6ircruag 592 14 produce produce VB cord-022235-6ircruag 592 15 a a DT cord-022235-6ircruag 592 16 low low JJ cord-022235-6ircruag 592 17 pH ph NN cord-022235-6ircruag 592 18 environment environment NN cord-022235-6ircruag 592 19 necessary necessary JJ cord-022235-6ircruag 592 20 for for IN cord-022235-6ircruag 592 21 sorting sort VBG cord-022235-6ircruag 592 22 or or CC cord-022235-6ircruag 592 23 receptor receptor NN cord-022235-6ircruag 592 24 dissociation dissociation NN cord-022235-6ircruag 592 25 , , , cord-022235-6ircruag 592 26 or or CC cord-022235-6ircruag 592 27 proteins protein NNS cord-022235-6ircruag 592 28 involved involve VBN cord-022235-6ircruag 592 29 in in IN cord-022235-6ircruag 592 30 vesicle vesicle NN cord-022235-6ircruag 592 31 fission fission NN cord-022235-6ircruag 592 32 and and CC cord-022235-6ircruag 592 33 fusion fusion NN cord-022235-6ircruag 592 34 . . . cord-022235-6ircruag 593 1 Much much RB cord-022235-6ircruag 593 2 less less JJR cord-022235-6ircruag 593 3 work work NN cord-022235-6ircruag 593 4 has have VBZ cord-022235-6ircruag 593 5 been be VBN cord-022235-6ircruag 593 6 done do VBN cord-022235-6ircruag 593 7 on on IN cord-022235-6ircruag 593 8 plant plant NN cord-022235-6ircruag 593 9 vacuolar vacuolar NNP cord-022235-6ircruag 593 10 proteins protein NNS cord-022235-6ircruag 593 11 . . . cord-022235-6ircruag 594 1 Tague Tague NNP cord-022235-6ircruag 594 2 and and CC cord-022235-6ircruag 594 3 Chrispeels Chrispeels NNP cord-022235-6ircruag 594 4 ( ( -LRB- cord-022235-6ircruag 594 5 1100 1100 CD cord-022235-6ircruag 594 6 ) ) -RRB- cord-022235-6ircruag 594 7 found find VBD cord-022235-6ircruag 594 8 that that IN cord-022235-6ircruag 594 9 the the DT cord-022235-6ircruag 594 10 plant plant NN cord-022235-6ircruag 594 11 vacuolar vacuolar NNP cord-022235-6ircruag 594 12 storage storage NN cord-022235-6ircruag 594 13 protein protein NN cord-022235-6ircruag 594 14 phytohemagglutinin phytohemagglutinin NNP cord-022235-6ircruag 594 15 was be VBD cord-022235-6ircruag 594 16 targeted target VBN cord-022235-6ircruag 594 17 mainly mainly RB cord-022235-6ircruag 594 18 to to IN cord-022235-6ircruag 594 19 the the DT cord-022235-6ircruag 594 20 vacuole vacuole NN cord-022235-6ircruag 594 21 when when WRB cord-022235-6ircruag 594 22 its -PRON- PRP$ cord-022235-6ircruag 594 23 structural structural JJ cord-022235-6ircruag 594 24 gene gene NN cord-022235-6ircruag 594 25 was be VBD cord-022235-6ircruag 594 26 expressed express VBN cord-022235-6ircruag 594 27 in in IN cord-022235-6ircruag 594 28 yeast yeast NN cord-022235-6ircruag 594 29 cells cell NNS cord-022235-6ircruag 594 30 . . . cord-022235-6ircruag 595 1 This this DT cord-022235-6ircruag 595 2 protein protein NN cord-022235-6ircruag 595 3 does do VBZ cord-022235-6ircruag 595 4 not not RB cord-022235-6ircruag 595 5 have have VB cord-022235-6ircruag 595 6 a a DT cord-022235-6ircruag 595 7 cleavable cleavable NN cord-022235-6ircruag 595 8 propeptide propeptide NN cord-022235-6ircruag 595 9 ( ( -LRB- cord-022235-6ircruag 595 10 it -PRON- PRP cord-022235-6ircruag 595 11 does do VBZ cord-022235-6ircruag 595 12 have have VB cord-022235-6ircruag 595 13 a a DT cord-022235-6ircruag 595 14 signal signal JJ cord-022235-6ircruag 595 15 peptide peptide NN cord-022235-6ircruag 595 16 , , , cord-022235-6ircruag 595 17 which which WDT cord-022235-6ircruag 595 18 was be VBD cord-022235-6ircruag 595 19 at at IN cord-022235-6ircruag 595 20 least least JJS cord-022235-6ircruag 595 21 partially partially RB cord-022235-6ircruag 595 22 processed process VBN cord-022235-6ircruag 595 23 in in IN cord-022235-6ircruag 595 24 yeast yeast NN cord-022235-6ircruag 595 25 cells cell NNS cord-022235-6ircruag 595 26 ) ) -RRB- cord-022235-6ircruag 595 27 , , , cord-022235-6ircruag 595 28 which which WDT cord-022235-6ircruag 595 29 means mean VBZ cord-022235-6ircruag 595 30 that that IN cord-022235-6ircruag 595 31 the the DT cord-022235-6ircruag 595 32 vacuolar vacuolar NNP cord-022235-6ircruag 595 33 plant plant NN cord-022235-6ircruag 595 34 sorting sorting NN cord-022235-6ircruag 595 35 signal signal NN cord-022235-6ircruag 595 36 which which WDT cord-022235-6ircruag 595 37 is be VBZ cord-022235-6ircruag 595 38 recognized recognize VBN cord-022235-6ircruag 595 39 by by IN cord-022235-6ircruag 595 40 the the DT cord-022235-6ircruag 595 41 yeast yeast NN cord-022235-6ircruag 595 42 vacuolar vacuolar NNP cord-022235-6ircruag 595 43 sorting sort VBG cord-022235-6ircruag 595 44 pathway pathway NN cord-022235-6ircruag 595 45 is be VBZ cord-022235-6ircruag 595 46 lo lo NNP cord-022235-6ircruag 595 47 cated cat VBN cord-022235-6ircruag 595 48 in in IN cord-022235-6ircruag 595 49 the the DT cord-022235-6ircruag 595 50 mature mature JJ cord-022235-6ircruag 595 51 part part NN cord-022235-6ircruag 595 52 of of IN cord-022235-6ircruag 595 53 the the DT cord-022235-6ircruag 595 54 phytohemagglutinin phytohemagglutinin NNP cord-022235-6ircruag 595 55 polypeptide polypeptide NN cord-022235-6ircruag 595 56 . . . cord-022235-6ircruag 596 1 mRNA mrna NN cord-022235-6ircruag 596 2 coding code VBG cord-022235-6ircruag 596 3 for for IN cord-022235-6ircruag 596 4 a a DT cord-022235-6ircruag 596 5 second second JJ cord-022235-6ircruag 596 6 plant plant NN cord-022235-6ircruag 596 7 storage storage NN cord-022235-6ircruag 596 8 protein protein NN cord-022235-6ircruag 596 9 , , , cord-022235-6ircruag 596 10 globulin globulin NN cord-022235-6ircruag 596 11 P p NN cord-022235-6ircruag 596 12 , , , cord-022235-6ircruag 596 13 has have VBZ cord-022235-6ircruag 596 14 been be VBN cord-022235-6ircruag 596 15 microinjected microinjecte VBN cord-022235-6ircruag 596 16 into into IN cord-022235-6ircruag 596 17 frog frog NN cord-022235-6ircruag 596 18 oocytes oocyte NNS cord-022235-6ircruag 596 19 , , , cord-022235-6ircruag 596 20 which which WDT cord-022235-6ircruag 596 21 secreted secrete VBD cord-022235-6ircruag 596 22 the the DT cord-022235-6ircruag 596 23 protein protein NN cord-022235-6ircruag 596 24 into into IN cord-022235-6ircruag 596 25 the the DT cord-022235-6ircruag 596 26 medium medium NN cord-022235-6ircruag 596 27 ( ( -LRB- cord-022235-6ircruag 596 28 51 51 CD cord-022235-6ircruag 596 29 ) ) -RRB- cord-022235-6ircruag 596 30 . . . cord-022235-6ircruag 597 1 This this DT cord-022235-6ircruag 597 2 confirms confirm VBZ cord-022235-6ircruag 597 3 that that IN cord-022235-6ircruag 597 4 plant plant NN cord-022235-6ircruag 597 5 vacuolar vacuolar NNP cord-022235-6ircruag 597 6 proteins protein NNS cord-022235-6ircruag 597 7 are be VBP cord-022235-6ircruag 597 8 bona bona JJ cord-022235-6ircruag 597 9 fide fide JJ cord-022235-6ircruag 597 10 secretory secretory JJ cord-022235-6ircruag 597 11 proteins protein NNS cord-022235-6ircruag 597 12 and and CC cord-022235-6ircruag 597 13 that that IN cord-022235-6ircruag 597 14 plant plant NN cord-022235-6ircruag 597 15 cells cell NNS cord-022235-6ircruag 597 16 have have VBP cord-022235-6ircruag 597 17 a a DT cord-022235-6ircruag 597 18 special special JJ cord-022235-6ircruag 597 19 branch branch NN cord-022235-6ircruag 597 20 of of IN cord-022235-6ircruag 597 21 the the DT cord-022235-6ircruag 597 22 secretory secretory JJ cord-022235-6ircruag 597 23 pathway pathway NN cord-022235-6ircruag 597 24 which which WDT cord-022235-6ircruag 597 25 shunts shunt VBZ cord-022235-6ircruag 597 26 storage storage NN cord-022235-6ircruag 597 27 proteins protein NNS cord-022235-6ircruag 597 28 into into IN cord-022235-6ircruag 597 29 vacuoles vacuole NNS cord-022235-6ircruag 597 30 . . . cord-022235-6ircruag 598 1 As as IN cord-022235-6ircruag 598 2 we -PRON- PRP cord-022235-6ircruag 598 3 have have VBP cord-022235-6ircruag 598 4 seen see VBN cord-022235-6ircruag 598 5 , , , cord-022235-6ircruag 598 6 protein protein NN cord-022235-6ircruag 598 7 - - HYPH cord-022235-6ircruag 598 8 coated coat VBN cord-022235-6ircruag 598 9 vesicles vesicle NNS cord-022235-6ircruag 598 10 are be VBP cord-022235-6ircruag 598 11 involved involve VBN cord-022235-6ircruag 598 12 in in IN cord-022235-6ircruag 598 13 transporting transport VBG cord-022235-6ircruag 598 14 secretory secretory JJ cord-022235-6ircruag 598 15 proteins protein NNS cord-022235-6ircruag 598 16 at at IN cord-022235-6ircruag 598 17 various various JJ cord-022235-6ircruag 598 18 stages stage NNS cord-022235-6ircruag 598 19 of of IN cord-022235-6ircruag 598 20 the the DT cord-022235-6ircruag 598 21 secretory secretory JJ cord-022235-6ircruag 598 22 pathway pathway NN cord-022235-6ircruag 598 23 . . . cord-022235-6ircruag 599 1 Some some DT cord-022235-6ircruag 599 2 vesi vesi NNP cord-022235-6ircruag 599 3 cles cles NNP cord-022235-6ircruag 599 4 ( ( -LRB- cord-022235-6ircruag 599 5 e.g. e.g. RB cord-022235-6ircruag 599 6 , , , cord-022235-6ircruag 599 7 lysosomal lysosomal JJ cord-022235-6ircruag 599 8 sorting sorting NN cord-022235-6ircruag 599 9 vesicles vesicle NNS cord-022235-6ircruag 599 10 and and CC cord-022235-6ircruag 599 11 immature immature JJ cord-022235-6ircruag 599 12 secretory secretory JJ cord-022235-6ircruag 599 13 granules granule NNS cord-022235-6ircruag 599 14 ) ) -RRB- cord-022235-6ircruag 599 15 , , , cord-022235-6ircruag 599 16 are be VBP cord-022235-6ircruag 599 17 coated coat VBN cord-022235-6ircruag 599 18 with with IN cord-022235-6ircruag 599 19 a a DT cord-022235-6ircruag 599 20 protein protein NN cord-022235-6ircruag 599 21 complex complex NN cord-022235-6ircruag 599 22 called call VBN cord-022235-6ircruag 599 23 clathrin clathrin NN cord-022235-6ircruag 599 24 , , , cord-022235-6ircruag 599 25 which which WDT cord-022235-6ircruag 599 26 also also RB cord-022235-6ircruag 599 27 coats coat VBZ cord-022235-6ircruag 599 28 endocytotic endocytotic JJ cord-022235-6ircruag 599 29 vesicles vesicle NNS cord-022235-6ircruag 599 30 ( ( -LRB- cord-022235-6ircruag 599 31 Chapter chapter NN cord-022235-6ircruag 599 32 VIII VIII NNP cord-022235-6ircruag 599 33 ) ) -RRB- cord-022235-6ircruag 599 34 . . . cord-022235-6ircruag 600 1 Other other JJ cord-022235-6ircruag 600 2 secretory secretory JJ cord-022235-6ircruag 600 3 vesicles vesicle NNS cord-022235-6ircruag 600 4 ( ( -LRB- cord-022235-6ircruag 601 1 e.g. e.g. RB cord-022235-6ircruag 601 2 , , , cord-022235-6ircruag 601 3 those those DT cord-022235-6ircruag 601 4 me -PRON- PRP cord-022235-6ircruag 601 5 diating diate VBG cord-022235-6ircruag 601 6 protein protein NN cord-022235-6ircruag 601 7 transport transport NN cord-022235-6ircruag 601 8 through through IN cord-022235-6ircruag 601 9 the the DT cord-022235-6ircruag 601 10 Golgi Golgi NNP cord-022235-6ircruag 601 11 ) ) -RRB- cord-022235-6ircruag 601 12 have have VB cord-022235-6ircruag 601 13 a a DT cord-022235-6ircruag 601 14 different different JJ cord-022235-6ircruag 601 15 type type NN cord-022235-6ircruag 601 16 of of IN cord-022235-6ircruag 601 17 pro pro JJ cord-022235-6ircruag 601 18 tein tein NNP cord-022235-6ircruag 601 19 coat coat NN cord-022235-6ircruag 601 20 ( ( -LRB- cord-022235-6ircruag 601 21 797 797 CD cord-022235-6ircruag 601 22 ) ) -RRB- cord-022235-6ircruag 601 23 . . . cord-022235-6ircruag 602 1 Clathrin Clathrin NNP cord-022235-6ircruag 602 2 , , , cord-022235-6ircruag 602 3 which which WDT cord-022235-6ircruag 602 4 is be VBZ cord-022235-6ircruag 602 5 composed compose VBN cord-022235-6ircruag 602 6 of of IN cord-022235-6ircruag 602 7 equimolar equimolar JJ cord-022235-6ircruag 602 8 amounts amount NNS cord-022235-6ircruag 602 9 of of IN cord-022235-6ircruag 602 10 heavy heavy JJ cord-022235-6ircruag 602 11 and and CC cord-022235-6ircruag 602 12 light light JJ cord-022235-6ircruag 602 13 chains chain NNS cord-022235-6ircruag 602 14 , , , cord-022235-6ircruag 602 15 forms form VBZ cord-022235-6ircruag 602 16 a a DT cord-022235-6ircruag 602 17 three three CD cord-022235-6ircruag 602 18 - - HYPH cord-022235-6ircruag 602 19 layered layered JJ cord-022235-6ircruag 602 20 cage cage NN cord-022235-6ircruag 602 21 which which WDT cord-022235-6ircruag 602 22 envelops envelop VBZ cord-022235-6ircruag 602 23 vesicles vesicle NNS cord-022235-6ircruag 602 24 in in IN cord-022235-6ircruag 602 25 a a DT cord-022235-6ircruag 602 26 shell shell NN cord-022235-6ircruag 602 27 with with IN cord-022235-6ircruag 602 28 fibrous fibrous JJ cord-022235-6ircruag 602 29 interconnections interconnection NNS cord-022235-6ircruag 602 30 , , , cord-022235-6ircruag 602 31 which which WDT cord-022235-6ircruag 602 32 give give VBP cord-022235-6ircruag 602 33 it -PRON- PRP cord-022235-6ircruag 602 34 mechanical mechanical JJ cord-022235-6ircruag 602 35 strength strength NN cord-022235-6ircruag 602 36 and and CC cord-022235-6ircruag 602 37 stability stability NN cord-022235-6ircruag 602 38 . . . cord-022235-6ircruag 603 1 The the DT cord-022235-6ircruag 603 2 vesicle vesicle NN cord-022235-6ircruag 603 3 membrane membrane NN cord-022235-6ircruag 603 4 is be VBZ cord-022235-6ircruag 603 5 thought think VBN cord-022235-6ircruag 603 6 to to TO cord-022235-6ircruag 603 7 have have VB cord-022235-6ircruag 603 8 receptors receptor NNS cord-022235-6ircruag 603 9 which which WDT cord-022235-6ircruag 603 10 anchor anchor VBD cord-022235-6ircruag 603 11 the the DT cord-022235-6ircruag 603 12 clathrin clathrin NN cord-022235-6ircruag 603 13 cage cage NN cord-022235-6ircruag 603 14 to to IN cord-022235-6ircruag 603 15 the the DT cord-022235-6ircruag 603 16 surface surface NN cord-022235-6ircruag 603 17 via via IN cord-022235-6ircruag 603 18 a a DT cord-022235-6ircruag 603 19 number number NN cord-022235-6ircruag 603 20 of of IN cord-022235-6ircruag 603 21 ancillary ancillary JJ cord-022235-6ircruag 603 22 assembly assembly NN cord-022235-6ircruag 603 23 proteins protein NNS cord-022235-6ircruag 603 24 which which WDT cord-022235-6ircruag 603 25 probably probably RB cord-022235-6ircruag 603 26 act act VBP cord-022235-6ircruag 603 27 as as IN cord-022235-6ircruag 603 28 bridges bridge NNS cord-022235-6ircruag 603 29 ( ( -LRB- cord-022235-6ircruag 603 30 1148 1148 CD cord-022235-6ircruag 603 31 , , , cord-022235-6ircruag 603 32 1149 1149 CD cord-022235-6ircruag 603 33 ) ) -RRB- cord-022235-6ircruag 603 34 . . . cord-022235-6ircruag 604 1 Different different JJ cord-022235-6ircruag 604 2 assembly assembly NN cord-022235-6ircruag 604 3 proteins protein NNS cord-022235-6ircruag 604 4 are be VBP cord-022235-6ircruag 604 5 found find VBN cord-022235-6ircruag 604 6 in in IN cord-022235-6ircruag 604 7 different different JJ cord-022235-6ircruag 604 8 classes class NNS cord-022235-6ircruag 604 9 of of IN cord-022235-6ircruag 604 10 clathrin clathrin NN cord-022235-6ircruag 604 11 - - HYPH cord-022235-6ircruag 604 12 coated coated JJ cord-022235-6ircruag 604 13 , , , cord-022235-6ircruag 604 14 TGN TGN NNP cord-022235-6ircruag 604 15 - - HYPH cord-022235-6ircruag 604 16 derived derive VBN cord-022235-6ircruag 604 17 , , , cord-022235-6ircruag 604 18 and and CC cord-022235-6ircruag 604 19 endocytic endocytic JJ cord-022235-6ircruag 604 20 vesicles vesicle NNS cord-022235-6ircruag 604 21 , , , cord-022235-6ircruag 604 22 suggesting suggest VBG cord-022235-6ircruag 604 23 that that IN cord-022235-6ircruag 604 24 they -PRON- PRP cord-022235-6ircruag 604 25 might may MD cord-022235-6ircruag 604 26 contribute contribute VB cord-022235-6ircruag 604 27 to to IN cord-022235-6ircruag 604 28 their -PRON- PRP$ cord-022235-6ircruag 604 29 respective respective JJ cord-022235-6ircruag 604 30 specificity specificity NN cord-022235-6ircruag 604 31 for for IN cord-022235-6ircruag 604 32 particular particular JJ cord-022235-6ircruag 604 33 membrane membrane NN cord-022235-6ircruag 604 34 targets target NNS cord-022235-6ircruag 604 35 ( ( -LRB- cord-022235-6ircruag 604 36 7 7 CD cord-022235-6ircruag 604 37 ) ) -RRB- cord-022235-6ircruag 604 38 . . . cord-022235-6ircruag 605 1 The the DT cord-022235-6ircruag 605 2 clathrin clathrin NN cord-022235-6ircruag 605 3 coat coat NN cord-022235-6ircruag 605 4 probably probably RB cord-022235-6ircruag 605 5 prevents prevent VBZ cord-022235-6ircruag 605 6 intimate intimate JJ cord-022235-6ircruag 605 7 contact contact NN cord-022235-6ircruag 605 8 between between IN cord-022235-6ircruag 605 9 fusing fuse VBG cord-022235-6ircruag 605 10 membranes membrane NNS cord-022235-6ircruag 605 11 and and CC cord-022235-6ircruag 605 12 must must MD cord-022235-6ircruag 605 13 thus thus RB cord-022235-6ircruag 605 14 be be VB cord-022235-6ircruag 605 15 removed remove VBN cord-022235-6ircruag 605 16 to to TO cord-022235-6ircruag 605 17 allow allow VB cord-022235-6ircruag 605 18 fusion fusion NN cord-022235-6ircruag 605 19 to to TO cord-022235-6ircruag 605 20 occur occur VB cord-022235-6ircruag 605 21 . . . cord-022235-6ircruag 606 1 This this DT cord-022235-6ircruag 606 2 may may MD cord-022235-6ircruag 606 3 explain explain VB cord-022235-6ircruag 606 4 , , , cord-022235-6ircruag 606 5 for for IN cord-022235-6ircruag 606 6 example example NN cord-022235-6ircruag 606 7 , , , cord-022235-6ircruag 606 8 the the DT cord-022235-6ircruag 606 9 disappearance disappearance NN cord-022235-6ircruag 606 10 of of IN cord-022235-6ircruag 606 11 the the DT cord-022235-6ircruag 606 12 clathrin clathrin NN cord-022235-6ircruag 606 13 coat coat NN cord-022235-6ircruag 606 14 from from IN cord-022235-6ircruag 606 15 matur matur JJ cord-022235-6ircruag 606 16 ing ing DT cord-022235-6ircruag 606 17 secretory secretory JJ cord-022235-6ircruag 606 18 granules granule NNS cord-022235-6ircruag 606 19 ( ( -LRB- cord-022235-6ircruag 606 20 Section section NN cord-022235-6ircruag 606 21 V.G.4.b V.G.4.b NNP cord-022235-6ircruag 606 22 ) ) -RRB- cord-022235-6ircruag 606 23 and and CC cord-022235-6ircruag 606 24 the the DT cord-022235-6ircruag 606 25 presence presence NN cord-022235-6ircruag 606 26 of of IN cord-022235-6ircruag 606 27 lysosomal lysosomal JJ cord-022235-6ircruag 606 28 protein protein NN cord-022235-6ircruag 606 29 precursors precursor NNS cord-022235-6ircruag 606 30 in in IN cord-022235-6ircruag 606 31 naked naked JJ cord-022235-6ircruag 606 32 as as RB cord-022235-6ircruag 606 33 well well RB cord-022235-6ircruag 606 34 as as IN cord-022235-6ircruag 606 35 coated coat VBN cord-022235-6ircruag 606 36 vesicles vesicle NNS cord-022235-6ircruag 606 37 . . . cord-022235-6ircruag 607 1 Vesicles Vesicles NNP cord-022235-6ircruag 607 2 coated coat VBN cord-022235-6ircruag 607 3 with with IN cord-022235-6ircruag 607 4 other other JJ cord-022235-6ircruag 607 5 proteins protein NNS cord-022235-6ircruag 607 6 are be VBP cord-022235-6ircruag 607 7 also also RB cord-022235-6ircruag 607 8 presumably presumably RB cord-022235-6ircruag 607 9 uncoated uncoated JJ cord-022235-6ircruag 607 10 to to TO cord-022235-6ircruag 607 11 allow allow VB cord-022235-6ircruag 607 12 fusion fusion NN cord-022235-6ircruag 607 13 to to IN cord-022235-6ircruag 607 14 oc oc NN cord-022235-6ircruag 607 15 cur cur NN cord-022235-6ircruag 607 16 . . . cord-022235-6ircruag 608 1 The the DT cord-022235-6ircruag 608 2 uncoating uncoating NN cord-022235-6ircruag 608 3 of of IN cord-022235-6ircruag 608 4 clathrin clathrin NN cord-022235-6ircruag 608 5 - - HYPH cord-022235-6ircruag 608 6 coated coat VBN cord-022235-6ircruag 608 7 vesicles vesicle NNS cord-022235-6ircruag 608 8 is be VBZ cord-022235-6ircruag 608 9 mediated mediate VBN cord-022235-6ircruag 608 10 by by IN cord-022235-6ircruag 608 11 the the DT cord-022235-6ircruag 608 12 ATPdependent atpdependent JJ cord-022235-6ircruag 608 13 cytosolic cytosolic JJ cord-022235-6ircruag 608 14 " " `` cord-022235-6ircruag 608 15 uncoating uncoating NN cord-022235-6ircruag 608 16 " " '' cord-022235-6ircruag 608 17 protein protein NN cord-022235-6ircruag 608 18 , , , cord-022235-6ircruag 608 19 which which WDT cord-022235-6ircruag 608 20 remains remain VBZ cord-022235-6ircruag 608 21 attached attach VBN cord-022235-6ircruag 608 22 to to IN cord-022235-6ircruag 608 23 the the DT cord-022235-6ircruag 608 24 released release VBN cord-022235-6ircruag 608 25 clathrin clathrin NN cord-022235-6ircruag 608 26 ( ( -LRB- cord-022235-6ircruag 608 27 1010 1010 CD cord-022235-6ircruag 608 28 ) ) -RRB- cord-022235-6ircruag 608 29 . . . cord-022235-6ircruag 609 1 Soluble soluble JJ cord-022235-6ircruag 609 2 clathrin clathrin NN cord-022235-6ircruag 609 3 retains retain VBZ cord-022235-6ircruag 609 4 its -PRON- PRP$ cord-022235-6ircruag 609 5 typical typical JJ cord-022235-6ircruag 609 6 triskelion triskelion NN cord-022235-6ircruag 609 7 con con NN cord-022235-6ircruag 609 8 formation formation NN cord-022235-6ircruag 609 9 . . . cord-022235-6ircruag 610 1 Uncoating uncoate VBG cord-022235-6ircruag 610 2 protein protein NN cord-022235-6ircruag 610 3 is be VBZ cord-022235-6ircruag 610 4 a a DT cord-022235-6ircruag 610 5 member member NN cord-022235-6ircruag 610 6 of of IN cord-022235-6ircruag 610 7 a a DT cord-022235-6ircruag 610 8 highly highly RB cord-022235-6ircruag 610 9 conserved conserved JJ cord-022235-6ircruag 610 10 group group NN cord-022235-6ircruag 610 11 of of IN cord-022235-6ircruag 610 12 stress stress NN cord-022235-6ircruag 610 13 proteins protein NNS cord-022235-6ircruag 610 14 and and CC cord-022235-6ircruag 610 15 may may MD cord-022235-6ircruag 610 16 be be VB cord-022235-6ircruag 610 17 related relate VBN cord-022235-6ircruag 610 18 to to IN cord-022235-6ircruag 610 19 HSP70 HSP70 NNP cord-022235-6ircruag 610 20 heat heat VB cord-022235-6ircruag 610 21 shock shock NN cord-022235-6ircruag 610 22 proteins protein NNS cord-022235-6ircruag 610 23 involved involve VBN cord-022235-6ircruag 610 24 in in IN cord-022235-6ircruag 610 25 other other JJ cord-022235-6ircruag 610 26 stages stage NNS cord-022235-6ircruag 610 27 in in IN cord-022235-6ircruag 610 28 secretory secretory JJ cord-022235-6ircruag 610 29 protein protein NN cord-022235-6ircruag 610 30 transport transport NN cord-022235-6ircruag 610 31 and and CC cord-022235-6ircruag 610 32 in in IN cord-022235-6ircruag 610 33 mitochondrial mitochondrial JJ cord-022235-6ircruag 610 34 protein protein NN cord-022235-6ircruag 610 35 import import NN cord-022235-6ircruag 610 36 ( ( -LRB- cord-022235-6ircruag 610 37 Sections section NNS cord-022235-6ircruag 610 38 III.C.3 iii.c.3 NN cord-022235-6ircruag 610 39 and and CC cord-022235-6ircruag 610 40 VLB.5 VLB.5 VBZ cord-022235-6ircruag 610 41 ) ) -RRB- cord-022235-6ircruag 610 42 . . . cord-022235-6ircruag 611 1 The the DT cord-022235-6ircruag 611 2 significance significance NN cord-022235-6ircruag 611 3 of of IN cord-022235-6ircruag 611 4 this this DT cord-022235-6ircruag 611 5 observation observation NN cord-022235-6ircruag 611 6 remains remain VBZ cord-022235-6ircruag 611 7 to to TO cord-022235-6ircruag 611 8 be be VB cord-022235-6ircruag 611 9 determined determine VBN cord-022235-6ircruag 611 10 ( ( -LRB- cord-022235-6ircruag 611 11 855,967 855,967 CD cord-022235-6ircruag 611 12 ) ) -RRB- cord-022235-6ircruag 611 13 , , , cord-022235-6ircruag 611 14 but but CC cord-022235-6ircruag 611 15 one one CD cord-022235-6ircruag 611 16 possibility possibility NN cord-022235-6ircruag 611 17 is be VBZ cord-022235-6ircruag 611 18 that that IN cord-022235-6ircruag 611 19 the the DT cord-022235-6ircruag 611 20 ATPase ATPase NNP cord-022235-6ircruag 611 21 ( ( -LRB- cord-022235-6ircruag 611 22 HSP70 HSP70 NNP cord-022235-6ircruag 611 23 ) ) -RRB- cord-022235-6ircruag 611 24 is be VBZ cord-022235-6ircruag 611 25 required require VBN cord-022235-6ircruag 611 26 to to TO cord-022235-6ircruag 611 27 activate activate VB cord-022235-6ircruag 611 28 an an DT cord-022235-6ircruag 611 29 uncoating uncoating NN cord-022235-6ircruag 611 30 enzyme enzyme NN cord-022235-6ircruag 611 31 which which WDT cord-022235-6ircruag 611 32 is be VBZ cord-022235-6ircruag 611 33 already already RB cord-022235-6ircruag 611 34 present present JJ cord-022235-6ircruag 611 35 in in IN cord-022235-6ircruag 611 36 the the DT cord-022235-6ircruag 611 37 clathrin clathrin NN cord-022235-6ircruag 611 38 complex complex NN cord-022235-6ircruag 611 39 . . . cord-022235-6ircruag 612 1 The the DT cord-022235-6ircruag 612 2 action action NN cord-022235-6ircruag 612 3 of of IN cord-022235-6ircruag 612 4 uncoating uncoate VBG cord-022235-6ircruag 612 5 protein protein NN cord-022235-6ircruag 612 6 must must MD cord-022235-6ircruag 612 7 be be VB cord-022235-6ircruag 612 8 triggered trigger VBN cord-022235-6ircruag 612 9 in in IN cord-022235-6ircruag 612 10 some some DT cord-022235-6ircruag 612 11 way way NN cord-022235-6ircruag 612 12 to to TO cord-022235-6ircruag 612 13 pre pre VB cord-022235-6ircruag 612 14 vent vent VB cord-022235-6ircruag 612 15 it -PRON- PRP cord-022235-6ircruag 612 16 from from IN cord-022235-6ircruag 612 17 destroying destroy VBG cord-022235-6ircruag 612 18 protein protein NN cord-022235-6ircruag 612 19 coats coat NNS cord-022235-6ircruag 612 20 on on IN cord-022235-6ircruag 612 21 immature immature JJ cord-022235-6ircruag 612 22 vesicles vesicle NNS cord-022235-6ircruag 612 23 or or CC cord-022235-6ircruag 612 24 on on IN cord-022235-6ircruag 612 25 coated coated JJ cord-022235-6ircruag 612 26 buds bud NNS cord-022235-6ircruag 612 27 , , , cord-022235-6ircruag 612 28 but but CC cord-022235-6ircruag 612 29 the the DT cord-022235-6ircruag 612 30 nature nature NN cord-022235-6ircruag 612 31 of of IN cord-022235-6ircruag 612 32 the the DT cord-022235-6ircruag 612 33 signal signal NN cord-022235-6ircruag 612 34 remains remain VBZ cord-022235-6ircruag 612 35 to to TO cord-022235-6ircruag 612 36 be be VB cord-022235-6ircruag 612 37 determined determine VBN cord-022235-6ircruag 612 38 . . . cord-022235-6ircruag 613 1 The the DT cord-022235-6ircruag 613 2 require require JJ cord-022235-6ircruag 613 3 ment ment NN cord-022235-6ircruag 613 4 for for IN cord-022235-6ircruag 613 5 ATP ATP NNP cord-022235-6ircruag 613 6 for for IN cord-022235-6ircruag 613 7 uncoating uncoating NN cord-022235-6ircruag 613 8 activity activity NN cord-022235-6ircruag 613 9 could could MD cord-022235-6ircruag 613 10 in in IN cord-022235-6ircruag 613 11 part part NN cord-022235-6ircruag 613 12 explain explain VB cord-022235-6ircruag 613 13 the the DT cord-022235-6ircruag 613 14 observed observed JJ cord-022235-6ircruag 613 15 requirement requirement NN cord-022235-6ircruag 613 16 for for IN cord-022235-6ircruag 613 17 the the DT cord-022235-6ircruag 613 18 same same JJ cord-022235-6ircruag 613 19 nucleotide nucleotide NN cord-022235-6ircruag 613 20 during during IN cord-022235-6ircruag 613 21 the the DT cord-022235-6ircruag 613 22 transport transport NN cord-022235-6ircruag 613 23 of of IN cord-022235-6ircruag 613 24 proteins protein NNS cord-022235-6ircruag 613 25 be be VBP cord-022235-6ircruag 613 26 tween tween VBG cord-022235-6ircruag 613 27 different different JJ cord-022235-6ircruag 613 28 stages stage NNS cord-022235-6ircruag 613 29 of of IN cord-022235-6ircruag 613 30 the the DT cord-022235-6ircruag 613 31 secretory secretory JJ cord-022235-6ircruag 613 32 pathway pathway NN cord-022235-6ircruag 613 33 if if IN cord-022235-6ircruag 613 34 a a DT cord-022235-6ircruag 613 35 similar similar JJ cord-022235-6ircruag 613 36 activity activity NN cord-022235-6ircruag 613 37 is be VBZ cord-022235-6ircruag 613 38 required require VBN cord-022235-6ircruag 613 39 to to TO cord-022235-6ircruag 613 40 remove remove VB cord-022235-6ircruag 613 41 other other JJ cord-022235-6ircruag 613 42 , , , cord-022235-6ircruag 613 43 nonclathrin nonclathrin JJ cord-022235-6ircruag 613 44 coats coat NNS cord-022235-6ircruag 613 45 . . . cord-022235-6ircruag 614 1 Attempts attempt NNS cord-022235-6ircruag 614 2 to to TO cord-022235-6ircruag 614 3 determine determine VB cord-022235-6ircruag 614 4 the the DT cord-022235-6ircruag 614 5 role role NN cord-022235-6ircruag 614 6 of of IN cord-022235-6ircruag 614 7 clathrin clathrin NN cord-022235-6ircruag 614 8 in in IN cord-022235-6ircruag 614 9 protein protein NN cord-022235-6ircruag 614 10 targeting target VBG cord-022235-6ircruag 614 11 in in IN cord-022235-6ircruag 614 12 yeast yeast NN cord-022235-6ircruag 614 13 cells cell NNS cord-022235-6ircruag 614 14 have have VBP cord-022235-6ircruag 614 15 given give VBN cord-022235-6ircruag 614 16 ambiguous ambiguous JJ cord-022235-6ircruag 614 17 results result NNS cord-022235-6ircruag 614 18 . . . cord-022235-6ircruag 615 1 Yeasts yeast NNS cord-022235-6ircruag 615 2 are be VBP cord-022235-6ircruag 615 3 known know VBN cord-022235-6ircruag 615 4 to to TO cord-022235-6ircruag 615 5 have have VB cord-022235-6ircruag 615 6 clathrin clathrin NN cord-022235-6ircruag 615 7 , , , cord-022235-6ircruag 615 8 and and CC cord-022235-6ircruag 615 9 coated coated JJ cord-022235-6ircruag 615 10 vesicles vesicle NNS cord-022235-6ircruag 615 11 have have VBP cord-022235-6ircruag 615 12 been be VBN cord-022235-6ircruag 615 13 observed observe VBN cord-022235-6ircruag 615 14 , , , cord-022235-6ircruag 615 15 but but CC cord-022235-6ircruag 615 16 it -PRON- PRP cord-022235-6ircruag 615 17 is be VBZ cord-022235-6ircruag 615 18 not not RB cord-022235-6ircruag 615 19 known know VBN cord-022235-6ircruag 615 20 whether whether IN cord-022235-6ircruag 615 21 clathrin clathrin NN cord-022235-6ircruag 615 22 coats coat NNS cord-022235-6ircruag 615 23 vesicles vesicle NNS cord-022235-6ircruag 615 24 involved involve VBN cord-022235-6ircruag 615 25 in in IN cord-022235-6ircruag 615 26 secretory secretory JJ cord-022235-6ircruag 615 27 protein protein NN cord-022235-6ircruag 615 28 targeting target VBG cord-022235-6ircruag 615 29 ( ( -LRB- cord-022235-6ircruag 615 30 1165 1165 CD cord-022235-6ircruag 615 31 ) ) -RRB- cord-022235-6ircruag 615 32 . . . cord-022235-6ircruag 616 1 The the DT cord-022235-6ircruag 616 2 gene gene NN cord-022235-6ircruag 616 3 for for IN cord-022235-6ircruag 616 4 the the DT cord-022235-6ircruag 616 5 clathrin clathrin NN cord-022235-6ircruag 616 6 heavy heavy JJ cord-022235-6ircruag 616 7 chain chain NN cord-022235-6ircruag 616 8 was be VBD cord-022235-6ircruag 616 9 independently independently RB cord-022235-6ircruag 616 10 cloned clone VBN cord-022235-6ircruag 616 11 by by IN cord-022235-6ircruag 616 12 two two CD cord-022235-6ircruag 616 13 groups group NNS cord-022235-6ircruag 616 14 who who WP cord-022235-6ircruag 616 15 used use VBD cord-022235-6ircruag 616 16 it -PRON- PRP cord-022235-6ircruag 616 17 to to TO cord-022235-6ircruag 616 18 inactivate inactivate VB cord-022235-6ircruag 616 19 the the DT cord-022235-6ircruag 616 20 chromosomal chromosomal JJ cord-022235-6ircruag 616 21 gene gene NN cord-022235-6ircruag 616 22 to to TO cord-022235-6ircruag 616 23 study study VB cord-022235-6ircruag 616 24 the the DT cord-022235-6ircruag 616 25 effect effect NN cord-022235-6ircruag 616 26 of of IN cord-022235-6ircruag 616 27 the the DT cord-022235-6ircruag 616 28 absence absence NN cord-022235-6ircruag 616 29 of of IN cord-022235-6ircruag 616 30 clathrin clathrin NN cord-022235-6ircruag 616 31 on on IN cord-022235-6ircruag 616 32 cell cell NN cord-022235-6ircruag 616 33 growth growth NN cord-022235-6ircruag 616 34 and and CC cord-022235-6ircruag 616 35 protein protein NN cord-022235-6ircruag 616 36 secretion secretion NN cord-022235-6ircruag 616 37 . . . cord-022235-6ircruag 617 1 Payne Payne NNP cord-022235-6ircruag 617 2 and and CC cord-022235-6ircruag 617 3 Schekman Schekman NNP cord-022235-6ircruag 617 4 ( ( -LRB- cord-022235-6ircruag 617 5 852 852 CD cord-022235-6ircruag 617 6 ) ) -RRB- cord-022235-6ircruag 617 7 and and CC cord-022235-6ircruag 617 8 Payne Payne NNP cord-022235-6ircruag 617 9 et et NNP cord-022235-6ircruag 617 10 al al NNP cord-022235-6ircruag 617 11 . . . cord-022235-6ircruag 618 1 ( ( -LRB- cord-022235-6ircruag 618 2 853,853a 853,853a LS cord-022235-6ircruag 618 3 ) ) -RRB- cord-022235-6ircruag 618 4 reported report VBD cord-022235-6ircruag 618 5 that that IN cord-022235-6ircruag 618 6 their -PRON- PRP$ cord-022235-6ircruag 618 7 mutants mutant NNS cord-022235-6ircruag 618 8 grew grow VBD cord-022235-6ircruag 618 9 somewhat somewhat RB cord-022235-6ircruag 618 10 more more RBR cord-022235-6ircruag 618 11 slowly slowly RB cord-022235-6ircruag 618 12 than than IN cord-022235-6ircruag 618 13 wild wild JJ cord-022235-6ircruag 618 14 - - HYPH cord-022235-6ircruag 618 15 type type NN cord-022235-6ircruag 618 16 cells cell NNS cord-022235-6ircruag 618 17 , , , cord-022235-6ircruag 618 18 secreted secrete VBD cord-022235-6ircruag 618 19 invertase invertase NN cord-022235-6ircruag 618 20 at at IN cord-022235-6ircruag 618 21 a a DT cord-022235-6ircruag 618 22 slightly slightly RB cord-022235-6ircruag 618 23 reduced reduce VBN cord-022235-6ircruag 618 24 rate rate NN cord-022235-6ircruag 618 25 , , , cord-022235-6ircruag 618 26 and and CC cord-022235-6ircruag 618 27 were be VBD cord-022235-6ircruag 618 28 partially partially RB cord-022235-6ircruag 618 29 defective defective JJ cord-022235-6ircruag 618 30 in in IN cord-022235-6ircruag 618 31 prepro prepro NN cord-022235-6ircruag 618 32 - - HYPH cord-022235-6ircruag 618 33 a a DT cord-022235-6ircruag 618 34 - - HYPH cord-022235-6ircruag 618 35 factor factor NN cord-022235-6ircruag 618 36 pro pro JJ cord-022235-6ircruag 618 37 - - JJ cord-022235-6ircruag 618 38 cessing cessing JJ cord-022235-6ircruag 618 39 . . . cord-022235-6ircruag 619 1 The the DT cord-022235-6ircruag 619 2 mutants mutant NNS cord-022235-6ircruag 619 3 accumulated accumulate VBD cord-022235-6ircruag 619 4 unusual unusual JJ cord-022235-6ircruag 619 5 vacuoles vacuole NNS cord-022235-6ircruag 619 6 , , , cord-022235-6ircruag 619 7 vesicles vesicle NNS cord-022235-6ircruag 619 8 , , , cord-022235-6ircruag 619 9 and and CC cord-022235-6ircruag 619 10 Golgiderived Golgiderived NNP cord-022235-6ircruag 619 11 structures structure NNS cord-022235-6ircruag 619 12 . . . cord-022235-6ircruag 620 1 These these DT cord-022235-6ircruag 620 2 results result NNS cord-022235-6ircruag 620 3 suggested suggest VBD cord-022235-6ircruag 620 4 that that IN cord-022235-6ircruag 620 5 the the DT cord-022235-6ircruag 620 6 absence absence NN cord-022235-6ircruag 620 7 of of IN cord-022235-6ircruag 620 8 clathrin clathrin NN cord-022235-6ircruag 620 9 did do VBD cord-022235-6ircruag 620 10 not not RB cord-022235-6ircruag 620 11 completely completely RB cord-022235-6ircruag 620 12 impair impair VB cord-022235-6ircruag 620 13 plasma plasma NN cord-022235-6ircruag 620 14 membrane membrane NN cord-022235-6ircruag 620 15 growth growth NN cord-022235-6ircruag 620 16 and and CC cord-022235-6ircruag 620 17 protein protein NN cord-022235-6ircruag 620 18 secretion secretion NN cord-022235-6ircruag 620 19 but but CC cord-022235-6ircruag 620 20 that that IN cord-022235-6ircruag 620 21 there there EX cord-022235-6ircruag 620 22 was be VBD cord-022235-6ircruag 620 23 nevertheless nevertheless RB cord-022235-6ircruag 620 24 a a DT cord-022235-6ircruag 620 25 reduced reduce VBN cord-022235-6ircruag 620 26 rate rate NN cord-022235-6ircruag 620 27 of of IN cord-022235-6ircruag 620 28 transit transit NN cord-022235-6ircruag 620 29 of of IN cord-022235-6ircruag 620 30 secretory secretory JJ cord-022235-6ircruag 620 31 proteins protein NNS cord-022235-6ircruag 620 32 through through IN cord-022235-6ircruag 620 33 the the DT cord-022235-6ircruag 620 34 later later JJ cord-022235-6ircruag 620 35 stages stage NNS cord-022235-6ircruag 620 36 of of IN cord-022235-6ircruag 620 37 the the DT cord-022235-6ircruag 620 38 secretory secretory JJ cord-022235-6ircruag 620 39 pathway pathway NN cord-022235-6ircruag 620 40 . . . cord-022235-6ircruag 621 1 Different different JJ cord-022235-6ircruag 621 2 results result NNS cord-022235-6ircruag 621 3 were be VBD cord-022235-6ircruag 621 4 obtained obtain VBN cord-022235-6ircruag 621 5 using use VBG cord-022235-6ircruag 621 6 exactly exactly RB cord-022235-6ircruag 621 7 the the DT cord-022235-6ircruag 621 8 same same JJ cord-022235-6ircruag 621 9 approach approach NN cord-022235-6ircruag 621 10 by by IN cord-022235-6ircruag 621 11 Lemmon Lemmon NNP cord-022235-6ircruag 621 12 and and CC cord-022235-6ircruag 621 13 Jones Jones NNP cord-022235-6ircruag 621 14 ( ( -LRB- cord-022235-6ircruag 621 15 627 627 CD cord-022235-6ircruag 621 16 ) ) -RRB- cord-022235-6ircruag 621 17 . . . cord-022235-6ircruag 622 1 They -PRON- PRP cord-022235-6ircruag 622 2 found find VBD cord-022235-6ircruag 622 3 that that IN cord-022235-6ircruag 622 4 cells cell NNS cord-022235-6ircruag 622 5 lacking lack VBG cord-022235-6ircruag 622 6 the the DT cord-022235-6ircruag 622 7 clathrin clathrin NN cord-022235-6ircruag 622 8 heavy heavy JJ cord-022235-6ircruag 622 9 chain chain NN cord-022235-6ircruag 622 10 were be VBD cord-022235-6ircruag 622 11 not not RB cord-022235-6ircruag 622 12 viable viable JJ cord-022235-6ircruag 622 13 unless unless IN cord-022235-6ircruag 622 14 they -PRON- PRP cord-022235-6ircruag 622 15 also also RB cord-022235-6ircruag 622 16 carried carry VBD cord-022235-6ircruag 622 17 a a DT cord-022235-6ircruag 622 18 suppressor suppressor NN cord-022235-6ircruag 622 19 mutation mutation NN cord-022235-6ircruag 622 20 . . . cord-022235-6ircruag 623 1 Even even RB cord-022235-6ircruag 623 2 with with IN cord-022235-6ircruag 623 3 this this DT cord-022235-6ircruag 623 4 mutation mutation NN cord-022235-6ircruag 623 5 , , , cord-022235-6ircruag 623 6 the the DT cord-022235-6ircruag 623 7 cells cell NNS cord-022235-6ircruag 623 8 grew grow VBD cord-022235-6ircruag 623 9 slowly slowly RB cord-022235-6ircruag 623 10 , , , cord-022235-6ircruag 623 11 were be VBD cord-022235-6ircruag 623 12 larger large JJR cord-022235-6ircruag 623 13 and and CC cord-022235-6ircruag 623 14 rounder round JJR cord-022235-6ircruag 623 15 , , , cord-022235-6ircruag 623 16 had have VBD cord-022235-6ircruag 623 17 an an DT cord-022235-6ircruag 623 18 unusual unusual JJ cord-022235-6ircruag 623 19 granular granular JJ cord-022235-6ircruag 623 20 appearance appearance NN cord-022235-6ircruag 623 21 , , , cord-022235-6ircruag 623 22 tended tend VBD cord-022235-6ircruag 623 23 to to TO cord-022235-6ircruag 623 24 aggregate aggregate VB cord-022235-6ircruag 623 25 in in IN cord-022235-6ircruag 623 26 liquid liquid JJ cord-022235-6ircruag 623 27 culture culture NN cord-022235-6ircruag 623 28 , , , cord-022235-6ircruag 623 29 and and CC cord-022235-6ircruag 623 30 were be VBD cord-022235-6ircruag 623 31 poly poly NNP cord-022235-6ircruag 623 32 ploid ploid NNP cord-022235-6ircruag 623 33 . . . cord-022235-6ircruag 624 1 These these DT cord-022235-6ircruag 624 2 results result NNS cord-022235-6ircruag 624 3 suggest suggest VBP cord-022235-6ircruag 624 4 that that IN cord-022235-6ircruag 624 5 the the DT cord-022235-6ircruag 624 6 absence absence NN cord-022235-6ircruag 624 7 of of IN cord-022235-6ircruag 624 8 clathrin clathrin NN cord-022235-6ircruag 624 9 is be VBZ cord-022235-6ircruag 624 10 highly highly RB cord-022235-6ircruag 624 11 detri detri JJ cord-022235-6ircruag 624 12 mental mental NN cord-022235-6ircruag 624 13 to to IN cord-022235-6ircruag 624 14 yeast yeast NN cord-022235-6ircruag 624 15 cells cell NNS cord-022235-6ircruag 624 16 , , , cord-022235-6ircruag 624 17 making make VBG cord-022235-6ircruag 624 18 it -PRON- PRP cord-022235-6ircruag 624 19 difficult difficult JJ cord-022235-6ircruag 624 20 to to TO cord-022235-6ircruag 624 21 determine determine VB cord-022235-6ircruag 624 22 whether whether IN cord-022235-6ircruag 624 23 clathrin clathrin NN cord-022235-6ircruag 624 24 plays play VBZ cord-022235-6ircruag 624 25 a a DT cord-022235-6ircruag 624 26 specific specific JJ cord-022235-6ircruag 624 27 role role NN cord-022235-6ircruag 624 28 in in IN cord-022235-6ircruag 624 29 protein protein NN cord-022235-6ircruag 624 30 secretion secretion NN cord-022235-6ircruag 624 31 in in IN cord-022235-6ircruag 624 32 this this DT cord-022235-6ircruag 624 33 organism organism NN cord-022235-6ircruag 624 34 . . . cord-022235-6ircruag 625 1 The the DT cord-022235-6ircruag 625 2 secretory secretory JJ cord-022235-6ircruag 625 3 pathway pathway NN cord-022235-6ircruag 625 4 is be VBZ cord-022235-6ircruag 625 5 almost almost RB cord-022235-6ircruag 625 6 certainly certainly RB cord-022235-6ircruag 625 7 the the DT cord-022235-6ircruag 625 8 route route NN cord-022235-6ircruag 625 9 by by IN cord-022235-6ircruag 625 10 which which WDT cord-022235-6ircruag 625 11 the the DT cord-022235-6ircruag 625 12 vast vast JJ cord-022235-6ircruag 625 13 majority majority NN cord-022235-6ircruag 625 14 of of IN cord-022235-6ircruag 625 15 secreted secrete VBN cord-022235-6ircruag 625 16 and and CC cord-022235-6ircruag 625 17 plasma plasma NN cord-022235-6ircruag 625 18 membrane membrane NN cord-022235-6ircruag 625 19 proteins protein NNS cord-022235-6ircruag 625 20 are be VBP cord-022235-6ircruag 625 21 exported export VBN cord-022235-6ircruag 625 22 by by IN cord-022235-6ircruag 625 23 eukaryotic eukaryotic JJ cord-022235-6ircruag 625 24 cells cell NNS cord-022235-6ircruag 625 25 . . . cord-022235-6ircruag 626 1 However however RB cord-022235-6ircruag 626 2 , , , cord-022235-6ircruag 626 3 there there EX cord-022235-6ircruag 626 4 is be VBZ cord-022235-6ircruag 626 5 increasing increase VBG cord-022235-6ircruag 626 6 evidence evidence NN cord-022235-6ircruag 626 7 that that IN cord-022235-6ircruag 626 8 some some DT cord-022235-6ircruag 626 9 plasma plasma NN cord-022235-6ircruag 626 10 membrane membrane NN cord-022235-6ircruag 626 11 and and CC cord-022235-6ircruag 626 12 secretory secretory JJ cord-022235-6ircruag 626 13 organelle organelle NN cord-022235-6ircruag 626 14 proteins protein NNS cord-022235-6ircruag 626 15 may may MD cord-022235-6ircruag 626 16 reach reach VB cord-022235-6ircruag 626 17 their -PRON- PRP$ cord-022235-6ircruag 626 18 final final JJ cord-022235-6ircruag 626 19 destina destina NNP cord-022235-6ircruag 626 20 tions tion NNS cord-022235-6ircruag 626 21 directly directly RB cord-022235-6ircruag 626 22 rather rather RB cord-022235-6ircruag 626 23 than than IN cord-022235-6ircruag 626 24 via via IN cord-022235-6ircruag 626 25 the the DT cord-022235-6ircruag 626 26 secretory secretory JJ cord-022235-6ircruag 626 27 pathway pathway NN cord-022235-6ircruag 626 28 . . . cord-022235-6ircruag 627 1 Examples example NNS cord-022235-6ircruag 627 2 of of IN cord-022235-6ircruag 627 3 this this DT cord-022235-6ircruag 627 4 class class NN cord-022235-6ircruag 627 5 of of IN cord-022235-6ircruag 627 6 proteins protein NNS cord-022235-6ircruag 627 7 include include VBP cord-022235-6ircruag 627 8 RAS RAS NNP cord-022235-6ircruag 627 9 - - HYPH cord-022235-6ircruag 627 10 like like JJ cord-022235-6ircruag 627 11 GTP GTP NNP cord-022235-6ircruag 627 12 binding bind VBG cord-022235-6ircruag 627 13 proteins protein NNS cord-022235-6ircruag 627 14 such such JJ cord-022235-6ircruag 627 15 as as IN cord-022235-6ircruag 627 16 SEC4 SEC4 NNP cord-022235-6ircruag 627 17 ( ( -LRB- cord-022235-6ircruag 627 18 987 987 CD cord-022235-6ircruag 627 19 ) ) -RRB- cord-022235-6ircruag 627 20 and and CC cord-022235-6ircruag 627 21 RAS2 RAS2 NNP cord-022235-6ircruag 627 22 ( ( -LRB- cord-022235-6ircruag 627 23 232 232 CD cord-022235-6ircruag 627 24 ) ) -RRB- cord-022235-6ircruag 627 25 of of IN cord-022235-6ircruag 627 26 the the DT cord-022235-6ircruag 627 27 yeast yeast NN cord-022235-6ircruag 627 28 S. S. NNP cord-022235-6ircruag 627 29 cerevisiae cerevisiae NNS cord-022235-6ircruag 627 30 and and CC cord-022235-6ircruag 627 31 similar similar JJ cord-022235-6ircruag 627 32 proteins protein NNS cord-022235-6ircruag 627 33 from from IN cord-022235-6ircruag 627 34 complex complex JJ cord-022235-6ircruag 627 35 eukaryotes eukaryote NNS cord-022235-6ircruag 627 36 ( ( -LRB- cord-022235-6ircruag 627 37 1194 1194 CD cord-022235-6ircruag 627 38 ) ) -RRB- cord-022235-6ircruag 627 39 , , , cord-022235-6ircruag 627 40 mating mate VBG cord-022235-6ircruag 627 41 pheromones pheromone NNS cord-022235-6ircruag 627 42 in in IN cord-022235-6ircruag 627 43 yeast yeast NN cord-022235-6ircruag 627 44 and and CC cord-022235-6ircruag 627 45 some some DT cord-022235-6ircruag 627 46 fungi fungus NNS cord-022235-6ircruag 627 47 ( ( -LRB- cord-022235-6ircruag 627 48 726 726 CD cord-022235-6ircruag 627 49 , , , cord-022235-6ircruag 627 50 886 886 CD cord-022235-6ircruag 627 51 , , , cord-022235-6ircruag 627 52 983 983 CD cord-022235-6ircruag 627 53 ) ) -RRB- cord-022235-6ircruag 627 54 , , , cord-022235-6ircruag 627 55 capsid capsid NN cord-022235-6ircruag 627 56 proteins protein NNS cord-022235-6ircruag 627 57 of of IN cord-022235-6ircruag 627 58 picornaviruses picornavirus NNS cord-022235-6ircruag 627 59 ( ( -LRB- cord-022235-6ircruag 627 60 851 851 CD cord-022235-6ircruag 627 61 ) ) -RRB- cord-022235-6ircruag 627 62 , , , cord-022235-6ircruag 627 63 and and CC cord-022235-6ircruag 627 64 src src NNP cord-022235-6ircruag 627 65 proteins protein NNS cord-022235-6ircruag 627 66 of of IN cord-022235-6ircruag 627 67 Rous rous JJ cord-022235-6ircruag 627 68 sarcoma sarcoma NN cord-022235-6ircruag 627 69 and and CC cord-022235-6ircruag 627 70 other other JJ cord-022235-6ircruag 627 71 transducing transducing NN cord-022235-6ircruag 627 72 viruses virus NNS cord-022235-6ircruag 627 73 ( ( -LRB- cord-022235-6ircruag 627 74 1029 1029 CD cord-022235-6ircruag 627 75 ) ) -RRB- cord-022235-6ircruag 627 76 . . . cord-022235-6ircruag 628 1 Most Most JJS cord-022235-6ircruag 628 2 if if IN cord-022235-6ircruag 628 3 not not RB cord-022235-6ircruag 628 4 all all DT cord-022235-6ircruag 628 5 of of IN cord-022235-6ircruag 628 6 these these DT cord-022235-6ircruag 628 7 proteins protein NNS cord-022235-6ircruag 628 8 are be VBP cord-022235-6ircruag 628 9 fatty fatty JJ cord-022235-6ircruag 628 10 acylated acylate VBN cord-022235-6ircruag 628 11 . . . cord-022235-6ircruag 629 1 Two two CD cord-022235-6ircruag 629 2 different different JJ cord-022235-6ircruag 629 3 amino amino NN cord-022235-6ircruag 629 4 acids acid NNS cord-022235-6ircruag 629 5 seem seem VBP cord-022235-6ircruag 629 6 to to TO cord-022235-6ircruag 629 7 be be VB cord-022235-6ircruag 629 8 modified modify VBN cord-022235-6ircruag 629 9 : : : cord-022235-6ircruag 629 10 N n CD cord-022235-6ircruag 629 11 - - HYPH cord-022235-6ircruag 629 12 terminal terminal NN cord-022235-6ircruag 629 13 glycines glycine NNS cord-022235-6ircruag 629 14 , , , cord-022235-6ircruag 629 15 which which WDT cord-022235-6ircruag 629 16 are be VBP cord-022235-6ircruag 629 17 substrates substrate NNS cord-022235-6ircruag 629 18 for for IN cord-022235-6ircruag 629 19 myristoyl myristoyl NNP cord-022235-6ircruag 629 20 CoA CoA NNP cord-022235-6ircruag 629 21 protein protein NNP cord-022235-6ircruag 629 22 7V 7V NNP cord-022235-6ircruag 629 23 - - HYPH cord-022235-6ircruag 629 24 myristoyltransferase myristoyltransferase NNP cord-022235-6ircruag 629 25 ( ( -LRB- cord-022235-6ircruag 629 26 1124 1124 CD cord-022235-6ircruag 629 27 ) ) -RRB- cord-022235-6ircruag 629 28 , , , cord-022235-6ircruag 629 29 and and CC cord-022235-6ircruag 629 30 C c NN cord-022235-6ircruag 629 31 - - HYPH cord-022235-6ircruag 629 32 terminal terminal NN cord-022235-6ircruag 629 33 cysteines cysteine NNS cord-022235-6ircruag 629 34 in in IN cord-022235-6ircruag 629 35 RASlike raslike JJ cord-022235-6ircruag 629 36 proteins protein NNS cord-022235-6ircruag 629 37 ( ( -LRB- cord-022235-6ircruag 629 38 232 232 CD cord-022235-6ircruag 629 39 ) ) -RRB- cord-022235-6ircruag 629 40 . . . cord-022235-6ircruag 630 1 These these DT cord-022235-6ircruag 630 2 Cys Cys NNP cord-022235-6ircruag 630 3 residues residue NNS cord-022235-6ircruag 630 4 are be VBP cord-022235-6ircruag 630 5 reported report VBN cord-022235-6ircruag 630 6 to to TO cord-022235-6ircruag 630 7 be be VB cord-022235-6ircruag 630 8 palmitoylated palmitoylate VBN cord-022235-6ircruag 630 9 , , , cord-022235-6ircruag 630 10 but but CC cord-022235-6ircruag 630 11 a a DT cord-022235-6ircruag 630 12 farnecyl farnecyl NN cord-022235-6ircruag 630 13 residue residue NN cord-022235-6ircruag 630 14 has have VBZ cord-022235-6ircruag 630 15 been be VBN cord-022235-6ircruag 630 16 found find VBN cord-022235-6ircruag 630 17 in in IN cord-022235-6ircruag 630 18 basidiomycete basidiomycete JJ cord-022235-6ircruag 630 19 pheromones pheromone NNS cord-022235-6ircruag 630 20 ( ( -LRB- cord-022235-6ircruag 630 21 983 983 CD cord-022235-6ircruag 630 22 ) ) -RRB- cord-022235-6ircruag 630 23 . . . cord-022235-6ircruag 631 1 The the DT cord-022235-6ircruag 631 2 absence absence NN cord-022235-6ircruag 631 3 of of IN cord-022235-6ircruag 631 4 the the DT cord-022235-6ircruag 631 5 fatty fatty JJ cord-022235-6ircruag 631 6 - - HYPH cord-022235-6ircruag 631 7 acylated acylate VBN cord-022235-6ircruag 631 8 amino amino NN cord-022235-6ircruag 631 9 acid acid NN cord-022235-6ircruag 631 10 disrupts disrupt VBZ cord-022235-6ircruag 631 11 membrane membrane NN cord-022235-6ircruag 631 12 associa associa NNP cord-022235-6ircruag 631 13 tion tion NN cord-022235-6ircruag 631 14 of of IN cord-022235-6ircruag 631 15 RAS RAS NNP cord-022235-6ircruag 631 16 and and CC cord-022235-6ircruag 631 17 src src NNP cord-022235-6ircruag 631 18 proteins protein NNS cord-022235-6ircruag 631 19 ( ( -LRB- cord-022235-6ircruag 631 20 232 232 CD cord-022235-6ircruag 631 21 , , , cord-022235-6ircruag 631 22 555 555 CD cord-022235-6ircruag 631 23 , , , cord-022235-6ircruag 631 24 1029 1029 CD cord-022235-6ircruag 631 25 , , , cord-022235-6ircruag 631 26 1194 1194 CD cord-022235-6ircruag 631 27 ) ) -RRB- cord-022235-6ircruag 631 28 , , , cord-022235-6ircruag 631 29 indicating indicate VBG cord-022235-6ircruag 631 30 that that IN cord-022235-6ircruag 631 31 fatty fatty JJ cord-022235-6ircruag 631 32 acids acid NNS cord-022235-6ircruag 631 33 probably probably RB cord-022235-6ircruag 631 34 anchor anchor VBD cord-022235-6ircruag 631 35 these these DT cord-022235-6ircruag 631 36 proteins protein NNS cord-022235-6ircruag 631 37 in in IN cord-022235-6ircruag 631 38 their -PRON- PRP$ cord-022235-6ircruag 631 39 respective respective JJ cord-022235-6ircruag 631 40 membranes membrane NNS cord-022235-6ircruag 631 41 . . . cord-022235-6ircruag 632 1 It -PRON- PRP cord-022235-6ircruag 632 2 remains remain VBZ cord-022235-6ircruag 632 3 to to TO cord-022235-6ircruag 632 4 be be VB cord-022235-6ircruag 632 5 determined determine VBN cord-022235-6ircruag 632 6 how how WRB cord-022235-6ircruag 632 7 fatty fatty JJ cord-022235-6ircruag 632 8 - - HYPH cord-022235-6ircruag 632 9 acylated acylate VBN cord-022235-6ircruag 632 10 proteins protein NNS cord-022235-6ircruag 632 11 actually actually RB cord-022235-6ircruag 632 12 cross cross VBP cord-022235-6ircruag 632 13 the the DT cord-022235-6ircruag 632 14 plasma plasma NN cord-022235-6ircruag 632 15 membrane membrane NN cord-022235-6ircruag 632 16 ( ( -LRB- cord-022235-6ircruag 632 17 as as IN cord-022235-6ircruag 632 18 in in IN cord-022235-6ircruag 632 19 the the DT cord-022235-6ircruag 632 20 case case NN cord-022235-6ircruag 632 21 of of IN cord-022235-6ircruag 632 22 the the DT cord-022235-6ircruag 632 23 fungal fungal JJ cord-022235-6ircruag 632 24 pheromones pheromone NNS cord-022235-6ircruag 632 25 ) ) -RRB- cord-022235-6ircruag 632 26 or or CC cord-022235-6ircruag 632 27 what what WP cord-022235-6ircruag 632 28 determines determine VBZ cord-022235-6ircruag 632 29 their -PRON- PRP$ cord-022235-6ircruag 632 30 specificity specificity NN cord-022235-6ircruag 632 31 for for IN cord-022235-6ircruag 632 32 certain certain JJ cord-022235-6ircruag 632 33 membranes membrane NNS cord-022235-6ircruag 632 34 . . . cord-022235-6ircruag 633 1 A a DT cord-022235-6ircruag 633 2 further further JJ cord-022235-6ircruag 633 3 example example NN cord-022235-6ircruag 633 4 of of IN cord-022235-6ircruag 633 5 a a DT cord-022235-6ircruag 633 6 secreted secrete VBN cord-022235-6ircruag 633 7 protein protein NN cord-022235-6ircruag 633 8 which which WDT cord-022235-6ircruag 633 9 does do VBZ cord-022235-6ircruag 633 10 not not RB cord-022235-6ircruag 633 11 have have VB cord-022235-6ircruag 633 12 a a DT cord-022235-6ircruag 633 13 secretory secretory JJ cord-022235-6ircruag 633 14 routing routing NN cord-022235-6ircruag 633 15 signal signal NN cord-022235-6ircruag 633 16 is be VBZ cord-022235-6ircruag 633 17 interleukin interleukin NN cord-022235-6ircruag 633 18 1 1 CD cord-022235-6ircruag 633 19 , , , cord-022235-6ircruag 633 20 but but CC cord-022235-6ircruag 633 21 very very RB cord-022235-6ircruag 633 22 little little JJ cord-022235-6ircruag 633 23 appears appear VBZ cord-022235-6ircruag 633 24 to to TO cord-022235-6ircruag 633 25 be be VB cord-022235-6ircruag 633 26 known know VBN cord-022235-6ircruag 633 27 about about IN cord-022235-6ircruag 633 28 how how WRB cord-022235-6ircruag 633 29 this this DT cord-022235-6ircruag 633 30 protein protein NN cord-022235-6ircruag 633 31 crosses cross VBZ cord-022235-6ircruag 633 32 the the DT cord-022235-6ircruag 633 33 plasma plasma NN cord-022235-6ircruag 633 34 membrane membrane NN cord-022235-6ircruag 633 35 . . . cord-022235-6ircruag 634 1 There there EX cord-022235-6ircruag 634 2 is be VBZ cord-022235-6ircruag 634 3 general general JJ cord-022235-6ircruag 634 4 agreement agreement NN cord-022235-6ircruag 634 5 concerning concern VBG cord-022235-6ircruag 634 6 the the DT cord-022235-6ircruag 634 7 events event NNS cord-022235-6ircruag 634 8 which which WDT cord-022235-6ircruag 634 9 lead lead VBP cord-022235-6ircruag 634 10 to to IN cord-022235-6ircruag 634 11 the the DT cord-022235-6ircruag 634 12 sort sort NN cord-022235-6ircruag 634 13 ing ing NN cord-022235-6ircruag 634 14 of of IN cord-022235-6ircruag 634 15 secretory secretory JJ cord-022235-6ircruag 634 16 proteins protein NNS cord-022235-6ircruag 634 17 into into IN cord-022235-6ircruag 634 18 different different JJ cord-022235-6ircruag 634 19 terminal terminal JJ cord-022235-6ircruag 634 20 branches branch NNS cord-022235-6ircruag 634 21 of of IN cord-022235-6ircruag 634 22 the the DT cord-022235-6ircruag 634 23 secretory secretory JJ cord-022235-6ircruag 634 24 pathway pathway NN cord-022235-6ircruag 634 25 , , , cord-022235-6ircruag 634 26 as as IN cord-022235-6ircruag 634 27 illustrated illustrate VBN cord-022235-6ircruag 634 28 in in IN cord-022235-6ircruag 634 29 Fig Fig NNP cord-022235-6ircruag 634 30 . . . cord-022235-6ircruag 635 1 V. V. NNP cord-022235-6ircruag 635 2 6 6 CD cord-022235-6ircruag 635 3 , , , cord-022235-6ircruag 635 4 but but CC cord-022235-6ircruag 635 5 we -PRON- PRP cord-022235-6ircruag 635 6 are be VBP cord-022235-6ircruag 635 7 clearly clearly RB cord-022235-6ircruag 635 8 a a DT cord-022235-6ircruag 635 9 long long JJ cord-022235-6ircruag 635 10 way way NN cord-022235-6ircruag 635 11 from from IN cord-022235-6ircruag 635 12 understanding understand VBG cord-022235-6ircruag 635 13 exactly exactly RB cord-022235-6ircruag 635 14 what what WP cord-022235-6ircruag 635 15 directs direct VBZ cord-022235-6ircruag 635 16 proteins protein NNS cord-022235-6ircruag 635 17 to to IN cord-022235-6ircruag 635 18 their -PRON- PRP$ cord-022235-6ircruag 635 19 specific specific JJ cord-022235-6ircruag 635 20 targets target NNS cord-022235-6ircruag 635 21 . . . cord-022235-6ircruag 636 1 Patch patch NN cord-022235-6ircruag 636 2 signals signal NNS cord-022235-6ircruag 636 3 are be VBP cord-022235-6ircruag 636 4 undoubtedly undoubtedly RB cord-022235-6ircruag 636 5 necessary necessary JJ cord-022235-6ircruag 636 6 for for IN cord-022235-6ircruag 636 7 sorting sort VBG cord-022235-6ircruag 636 8 soluble soluble JJ cord-022235-6ircruag 636 9 proteins protein NNS cord-022235-6ircruag 636 10 ( ( -LRB- cord-022235-6ircruag 636 11 other other JJ cord-022235-6ircruag 636 12 than than IN cord-022235-6ircruag 636 13 lysosomal lysosomal JJ cord-022235-6ircruag 636 14 enzymes enzyme NNS cord-022235-6ircruag 636 15 ) ) -RRB- cord-022235-6ircruag 636 16 into into IN cord-022235-6ircruag 636 17 the the DT cord-022235-6ircruag 636 18 various various JJ cord-022235-6ircruag 636 19 branches branch NNS cord-022235-6ircruag 636 20 of of IN cord-022235-6ircruag 636 21 the the DT cord-022235-6ircruag 636 22 secretory secretory JJ cord-022235-6ircruag 636 23 pathway pathway NN cord-022235-6ircruag 636 24 , , , cord-022235-6ircruag 636 25 but but CC cord-022235-6ircruag 636 26 these these DT cord-022235-6ircruag 636 27 will will MD cord-022235-6ircruag 636 28 be be VB cord-022235-6ircruag 636 29 difficult difficult JJ cord-022235-6ircruag 636 30 to to TO cord-022235-6ircruag 636 31 identify identify VB cord-022235-6ircruag 636 32 by by IN cord-022235-6ircruag 636 33 gene gene NN cord-022235-6ircruag 636 34 fusion fusion NN cord-022235-6ircruag 636 35 techniques technique NNS cord-022235-6ircruag 636 36 . . . cord-022235-6ircruag 637 1 At at IN cord-022235-6ircruag 637 2 present present NN cord-022235-6ircruag 637 3 , , , cord-022235-6ircruag 637 4 we -PRON- PRP cord-022235-6ircruag 637 5 have have VBP cord-022235-6ircruag 637 6 no no DT cord-022235-6ircruag 637 7 clear clear JJ cord-022235-6ircruag 637 8 idea idea NN cord-022235-6ircruag 637 9 of of IN cord-022235-6ircruag 637 10 the the DT cord-022235-6ircruag 637 11 extent extent NN cord-022235-6ircruag 637 12 to to TO cord-022235-6ircruag 637 13 which which WDT cord-022235-6ircruag 637 14 the the DT cord-022235-6ircruag 637 15 sorting sorting NN cord-022235-6ircruag 637 16 vesicles vesicle NNS cord-022235-6ircruag 637 17 have have VBP cord-022235-6ircruag 637 18 different different JJ cord-022235-6ircruag 637 19 membrane membrane NN cord-022235-6ircruag 637 20 contents content NNS cord-022235-6ircruag 637 21 , , , cord-022235-6ircruag 637 22 but but CC cord-022235-6ircruag 637 23 it -PRON- PRP cord-022235-6ircruag 637 24 seems seem VBZ cord-022235-6ircruag 637 25 probable probable JJ cord-022235-6ircruag 637 26 that that IN cord-022235-6ircruag 637 27 specific specific JJ cord-022235-6ircruag 637 28 groups group NNS cord-022235-6ircruag 637 29 of of IN cord-022235-6ircruag 637 30 membrane membrane NN cord-022235-6ircruag 637 31 proteins protein NNS cord-022235-6ircruag 637 32 ( ( -LRB- cord-022235-6ircruag 637 33 lysosomal lysosomal JJ cord-022235-6ircruag 637 34 , , , cord-022235-6ircruag 637 35 secretory secretory JJ cord-022235-6ircruag 637 36 granule granule NN cord-022235-6ircruag 637 37 , , , cord-022235-6ircruag 637 38 apical apical JJ cord-022235-6ircruag 637 39 , , , cord-022235-6ircruag 637 40 and and CC cord-022235-6ircruag 637 41 basolateral basolateral JJ cord-022235-6ircruag 637 42 ) ) -RRB- cord-022235-6ircruag 637 43 accumulate accumulate VBP cord-022235-6ircruag 637 44 at at IN cord-022235-6ircruag 637 45 different different JJ cord-022235-6ircruag 637 46 sites site NNS cord-022235-6ircruag 637 47 in in IN cord-022235-6ircruag 637 48 the the DT cord-022235-6ircruag 637 49 membrane membrane NN cord-022235-6ircruag 637 50 of of IN cord-022235-6ircruag 637 51 the the DT cord-022235-6ircruag 637 52 TGN TGN NNP cord-022235-6ircruag 637 53 from from IN cord-022235-6ircruag 637 54 which which WDT cord-022235-6ircruag 637 55 sorting sort VBG cord-022235-6ircruag 637 56 vesicles vesicle NNS cord-022235-6ircruag 637 57 bud bud NN cord-022235-6ircruag 637 58 . . . cord-022235-6ircruag 638 1 This this DT cord-022235-6ircruag 638 2 specialization specialization NN cord-022235-6ircruag 638 3 is be VBZ cord-022235-6ircruag 638 4 presumably presumably RB cord-022235-6ircruag 638 5 also also RB cord-022235-6ircruag 638 6 determined determine VBN cord-022235-6ircruag 638 7 by by IN cord-022235-6ircruag 638 8 protein protein NN cord-022235-6ircruag 638 9 - - HYPH cord-022235-6ircruag 638 10 protein protein NN cord-022235-6ircruag 638 11 interactions interaction NNS cord-022235-6ircruag 638 12 , , , cord-022235-6ircruag 638 13 but but CC cord-022235-6ircruag 638 14 the the DT cord-022235-6ircruag 638 15 possibility possibility NN cord-022235-6ircruag 638 16 that that IN cord-022235-6ircruag 638 17 other other JJ cord-022235-6ircruag 638 18 interactions interaction NNS cord-022235-6ircruag 638 19 ( ( -LRB- cord-022235-6ircruag 638 20 e.g. e.g. RB cord-022235-6ircruag 638 21 , , , cord-022235-6ircruag 638 22 protein protein NN cord-022235-6ircruag 638 23 - - HYPH cord-022235-6ircruag 638 24 lipid lipid NN cord-022235-6ircruag 638 25 ) ) -RRB- cord-022235-6ircruag 638 26 might may MD cord-022235-6ircruag 638 27 be be VB cord-022235-6ircruag 638 28 involved involve VBN cord-022235-6ircruag 638 29 should should MD cord-022235-6ircruag 638 30 not not RB cord-022235-6ircruag 638 31 be be VB cord-022235-6ircruag 638 32 over over IN cord-022235-6ircruag 638 33 looked look VBN cord-022235-6ircruag 638 34 . . . cord-022235-6ircruag 639 1 Another another DT cord-022235-6ircruag 639 2 interesting interesting JJ cord-022235-6ircruag 639 3 observation observation NN cord-022235-6ircruag 639 4 is be VBZ cord-022235-6ircruag 639 5 that that IN cord-022235-6ircruag 639 6 ATP ATP NNP cord-022235-6ircruag 639 7 seems seem VBZ cord-022235-6ircruag 639 8 to to TO cord-022235-6ircruag 639 9 be be VB cord-022235-6ircruag 639 10 required require VBN cord-022235-6ircruag 639 11 at at IN cord-022235-6ircruag 639 12 almost almost RB cord-022235-6ircruag 639 13 every every DT cord-022235-6ircruag 639 14 stage stage NN cord-022235-6ircruag 639 15 in in IN cord-022235-6ircruag 639 16 the the DT cord-022235-6ircruag 639 17 secretory secretory JJ cord-022235-6ircruag 639 18 pathway pathway NN cord-022235-6ircruag 639 19 . . . cord-022235-6ircruag 640 1 ATP ATP NNP cord-022235-6ircruag 640 2 has have VBZ cord-022235-6ircruag 640 3 been be VBN cord-022235-6ircruag 640 4 proposed propose VBN cord-022235-6ircruag 640 5 to to TO cord-022235-6ircruag 640 6 act act VB cord-022235-6ircruag 640 7 in in IN cord-022235-6ircruag 640 8 a a DT cord-022235-6ircruag 640 9 variety variety NN cord-022235-6ircruag 640 10 of of IN cord-022235-6ircruag 640 11 ways way NNS cord-022235-6ircruag 640 12 , , , cord-022235-6ircruag 640 13 including include VBG cord-022235-6ircruag 640 14 acidification acidification NN cord-022235-6ircruag 640 15 of of IN cord-022235-6ircruag 640 16 secretory secretory JJ cord-022235-6ircruag 640 17 organelles organelle NNS cord-022235-6ircruag 640 18 and and CC cord-022235-6ircruag 640 19 vesicles vesicle NNS cord-022235-6ircruag 640 20 , , , cord-022235-6ircruag 640 21 phosphorylation phosphorylation NN cord-022235-6ircruag 640 22 of of IN cord-022235-6ircruag 640 23 receptors receptor NNS cord-022235-6ircruag 640 24 or or CC cord-022235-6ircruag 640 25 ligands ligand NNS cord-022235-6ircruag 640 26 , , , cord-022235-6ircruag 640 27 protein protein NN cord-022235-6ircruag 640 28 folding folding NN cord-022235-6ircruag 640 29 and and CC cord-022235-6ircruag 640 30 " " `` cord-022235-6ircruag 640 31 proofreading proofreading NN cord-022235-6ircruag 640 32 , , , cord-022235-6ircruag 640 33 " " '' cord-022235-6ircruag 640 34 activation activation NN cord-022235-6ircruag 640 35 of of IN cord-022235-6ircruag 640 36 cytoskeletal cytoskeletal JJ cord-022235-6ircruag 640 37 motors motor NNS cord-022235-6ircruag 640 38 , , , cord-022235-6ircruag 640 39 and and CC cord-022235-6ircruag 640 40 vesicle vesicle NN cord-022235-6ircruag 640 41 uncoating uncoating NN cord-022235-6ircruag 640 42 activity activity NN cord-022235-6ircruag 640 43 . . . cord-022235-6ircruag 641 1 There there EX cord-022235-6ircruag 641 2 is be VBZ cord-022235-6ircruag 641 3 increasing increase VBG cord-022235-6ircruag 641 4 evidence evidence NN cord-022235-6ircruag 641 5 that that IN cord-022235-6ircruag 641 6 GTP GTP NNP cord-022235-6ircruag 641 7 and and CC cord-022235-6ircruag 641 8 GTP GTP NNP cord-022235-6ircruag 641 9 binding bind VBG cord-022235-6ircruag 641 10 proteins protein NNS cord-022235-6ircruag 641 11 ( ( -LRB- cord-022235-6ircruag 641 12 G g NN cord-022235-6ircruag 641 13 proteins protein NNS cord-022235-6ircruag 641 14 ) ) -RRB- cord-022235-6ircruag 641 15 are be VBP cord-022235-6ircruag 641 16 also also RB cord-022235-6ircruag 641 17 involved involve VBN cord-022235-6ircruag 641 18 at at IN cord-022235-6ircruag 641 19 several several JJ cord-022235-6ircruag 641 20 stages stage NNS cord-022235-6ircruag 641 21 in in IN cord-022235-6ircruag 641 22 the the DT cord-022235-6ircruag 641 23 secretory secretory JJ cord-022235-6ircruag 641 24 pathway pathway NN cord-022235-6ircruag 641 25 . . . cord-022235-6ircruag 642 1 GTP gtp NN cord-022235-6ircruag 642 2 binding bind VBG cord-022235-6ircruag 642 3 proteins protein NNS cord-022235-6ircruag 642 4 are be VBP cord-022235-6ircruag 642 5 also also RB cord-022235-6ircruag 642 6 known know VBN cord-022235-6ircruag 642 7 to to TO cord-022235-6ircruag 642 8 be be VB cord-022235-6ircruag 642 9 involved involve VBN cord-022235-6ircruag 642 10 in in IN cord-022235-6ircruag 642 11 the the DT cord-022235-6ircruag 642 12 generation generation NN cord-022235-6ircruag 642 13 of of IN cord-022235-6ircruag 642 14 other other JJ cord-022235-6ircruag 642 15 intracellular intracellular JJ cord-022235-6ircruag 642 16 signals signal NNS cord-022235-6ircruag 642 17 , , , cord-022235-6ircruag 642 18 such such JJ cord-022235-6ircruag 642 19 as as IN cord-022235-6ircruag 642 20 the the DT cord-022235-6ircruag 642 21 activation activation NN cord-022235-6ircruag 642 22 or or CC cord-022235-6ircruag 642 23 inactivation inactivation NN cord-022235-6ircruag 642 24 of of IN cord-022235-6ircruag 642 25 adeny adeny NNP cord-022235-6ircruag 642 26 late late JJ cord-022235-6ircruag 642 27 cyclase cyclase NNP cord-022235-6ircruag 642 28 , , , cord-022235-6ircruag 642 29 the the DT cord-022235-6ircruag 642 30 activation activation NN cord-022235-6ircruag 642 31 of of IN cord-022235-6ircruag 642 32 cyclic cyclic JJ cord-022235-6ircruag 642 33 GMP GMP NNP cord-022235-6ircruag 642 34 phosphodiesterase phosphodiesterase NN cord-022235-6ircruag 642 35 , , , cord-022235-6ircruag 642 36 and and CC cord-022235-6ircruag 642 37 the the DT cord-022235-6ircruag 642 38 control control NN cord-022235-6ircruag 642 39 of of IN cord-022235-6ircruag 642 40 phospholipase phospholipase NNP cord-022235-6ircruag 642 41 C C NNP cord-022235-6ircruag 642 42 action action NN cord-022235-6ircruag 642 43 ( ( -LRB- cord-022235-6ircruag 642 44 769 769 CD cord-022235-6ircruag 642 45 ) ) -RRB- cord-022235-6ircruag 642 46 . . . cord-022235-6ircruag 643 1 By by IN cord-022235-6ircruag 643 2 analogy analogy NN cord-022235-6ircruag 643 3 , , , cord-022235-6ircruag 643 4 GTP GTP NNP cord-022235-6ircruag 643 5 binding bind VBG cord-022235-6ircruag 643 6 pro pro FW cord-022235-6ircruag 643 7 teins tein NNS cord-022235-6ircruag 643 8 may may MD cord-022235-6ircruag 643 9 act act VB cord-022235-6ircruag 643 10 as as IN cord-022235-6ircruag 643 11 signals signal NNS cord-022235-6ircruag 643 12 or or CC cord-022235-6ircruag 643 13 to to TO cord-022235-6ircruag 643 14 activate activate VB cord-022235-6ircruag 643 15 receptors receptor NNS cord-022235-6ircruag 643 16 or or CC cord-022235-6ircruag 643 17 ligands ligand NNS cord-022235-6ircruag 643 18 to to TO cord-022235-6ircruag 643 19 ensure ensure VB cord-022235-6ircruag 643 20 vectorial vectorial JJ cord-022235-6ircruag 643 21 transport transport NN cord-022235-6ircruag 643 22 through through IN cord-022235-6ircruag 643 23 the the DT cord-022235-6ircruag 643 24 secretory secretory JJ cord-022235-6ircruag 643 25 pathway pathway NN cord-022235-6ircruag 643 26 ( ( -LRB- cord-022235-6ircruag 643 27 109 109 CD cord-022235-6ircruag 643 28 ) ) -RRB- cord-022235-6ircruag 643 29 . . . cord-022235-6ircruag 644 1 Constitutive constitutive JJ cord-022235-6ircruag 644 2 and and CC cord-022235-6ircruag 644 3 regulated regulated JJ cord-022235-6ircruag 644 4 secretion secretion NN cord-022235-6ircruag 644 5 of of IN cord-022235-6ircruag 644 6 proteins protein NNS cord-022235-6ircruag 644 7 Progress progress VBP cord-022235-6ircruag 644 8 in in IN cord-022235-6ircruag 644 9 unravelling unravel VBG cord-022235-6ircruag 644 10 pathways pathway NNS cord-022235-6ircruag 644 11 , , , cord-022235-6ircruag 644 12 of of IN cord-022235-6ircruag 644 13 Golgi Golgi NNP cord-022235-6ircruag 644 14 traffic traffic NN cord-022235-6ircruag 644 15 Assembly Assembly NNP cord-022235-6ircruag 644 16 of of IN cord-022235-6ircruag 644 17 asparagine asparagine NN cord-022235-6ircruag 644 18 - - HYPH cord-022235-6ircruag 644 19 linked link VBN cord-022235-6ircruag 644 20 oligosaccharides oligosaccharide NNS cord-022235-6ircruag 645 1 The the DT cord-022235-6ircruag 645 2 sorting sorting NN cord-022235-6ircruag 645 3 of of IN cord-022235-6ircruag 645 4 proteins protein NNS cord-022235-6ircruag 645 5 to to IN cord-022235-6ircruag 645 6 the the DT cord-022235-6ircruag 645 7 plasma plasma NN cord-022235-6ircruag 645 8 membrane membrane NN cord-022235-6ircruag 645 9 in in IN cord-022235-6ircruag 645 10 epithelial epithelial JJ cord-022235-6ircruag 645 11 cells cell NNS cord-022235-6ircruag 645 12 Biosynthetic biosynthetic JJ cord-022235-6ircruag 645 13 protein protein NN cord-022235-6ircruag 645 14 transport transport NN cord-022235-6ircruag 645 15 and and CC cord-022235-6ircruag 645 16 sorting sorting NN cord-022235-6ircruag 645 17 by by IN cord-022235-6ircruag 645 18 the the DT cord-022235-6ircruag 645 19 endoplasmic endoplasmic NN cord-022235-6ircruag 645 20 reticulum reticulum NN cord-022235-6ircruag 645 21 and and CC cord-022235-6ircruag 645 22 Golgi Golgi NNP cord-022235-6ircruag 645 23 Protein Protein NNP cord-022235-6ircruag 645 24 localization localization NN cord-022235-6ircruag 645 25 and and CC cord-022235-6ircruag 645 26 membrane membrane NN cord-022235-6ircruag 645 27 traffic traffic NN cord-022235-6ircruag 645 28 in in IN cord-022235-6ircruag 645 29 yeast yeast NN cord-022235-6ircruag 645 30 Lysosomal Lysosomal NNP cord-022235-6ircruag 645 31 enzymes enzyme NNS cord-022235-6ircruag 645 32 and and CC cord-022235-6ircruag 645 33 their -PRON- PRP$ cord-022235-6ircruag 645 34 receptors receptor NNS