id sid tid token lemma pos cord-014901-d9szap94 1 1 key key NN cord-014901-d9szap94 1 2 : : : cord-014901-d9szap94 1 3 cord-014901-d9szap94 cord-014901-d9szap94 ADD cord-014901-d9szap94 2 1 authors author NNS cord-014901-d9szap94 2 2 : : : cord-014901-d9szap94 2 3 Permyakova Permyakova NNP cord-014901-d9szap94 2 4 , , , cord-014901-d9szap94 2 5 N. N. NNP cord-014901-d9szap94 2 6 V. V. NNP cord-014901-d9szap94 2 7 ; ; : cord-014901-d9szap94 2 8 Uvarova Uvarova NNP cord-014901-d9szap94 2 9 , , , cord-014901-d9szap94 2 10 E. E. NNP cord-014901-d9szap94 2 11 A. a. NN cord-014901-d9szap94 2 12 ; ; : cord-014901-d9szap94 2 13 Deineko Deineko NNP cord-014901-d9szap94 2 14 , , , cord-014901-d9szap94 2 15 E. E. NNP cord-014901-d9szap94 2 16 V. V. NNP cord-014901-d9szap94 2 17 title title NN cord-014901-d9szap94 2 18 : : : cord-014901-d9szap94 2 19 State state NN cord-014901-d9szap94 2 20 of of IN cord-014901-d9szap94 2 21 research research NN cord-014901-d9szap94 2 22 in in IN cord-014901-d9szap94 2 23 the the DT cord-014901-d9szap94 2 24 field field NN cord-014901-d9szap94 2 25 of of IN cord-014901-d9szap94 2 26 the the DT cord-014901-d9szap94 2 27 creation creation NN cord-014901-d9szap94 2 28 of of IN cord-014901-d9szap94 2 29 plant plant NN cord-014901-d9szap94 2 30 vaccines vaccine NNS cord-014901-d9szap94 2 31 for for IN cord-014901-d9szap94 2 32 veterinary veterinary JJ cord-014901-d9szap94 2 33 use use NN cord-014901-d9szap94 2 34 date date NN cord-014901-d9szap94 2 35 : : : cord-014901-d9szap94 2 36 2015 2015 CD cord-014901-d9szap94 2 37 - - HYPH cord-014901-d9szap94 2 38 01 01 CD cord-014901-d9szap94 2 39 - - HYPH cord-014901-d9szap94 2 40 04 04 CD cord-014901-d9szap94 2 41 journal journal NN cord-014901-d9szap94 2 42 : : : cord-014901-d9szap94 3 1 Russ Russ NNP cord-014901-d9szap94 3 2 J J NNP cord-014901-d9szap94 3 3 Plant Plant NNP cord-014901-d9szap94 3 4 Physiol Physiol NNP cord-014901-d9szap94 3 5 DOI DOI NNP cord-014901-d9szap94 3 6 : : : cord-014901-d9szap94 4 1 10.1134 10.1134 CD cord-014901-d9szap94 4 2 / / SYM cord-014901-d9szap94 4 3 s1021443715010100 s1021443715010100 NN cord-014901-d9szap94 4 4 sha sha NNP cord-014901-d9szap94 4 5 : : : cord-014901-d9szap94 5 1 97a4513a333a020295682ac5d16dc9ced94f093d 97a4513a333a020295682ac5d16dc9ced94f093d CD cord-014901-d9szap94 5 2 doc_id doc_id CD cord-014901-d9szap94 5 3 : : : cord-014901-d9szap94 5 4 14901 14901 CD cord-014901-d9szap94 5 5 cord_uid cord_uid NNS cord-014901-d9szap94 5 6 : : : cord-014901-d9szap94 6 1 d9szap94 d9szap94 NNP cord-014901-d9szap94 6 2 Transgenic transgenic JJ cord-014901-d9szap94 6 3 plants plant NNS cord-014901-d9szap94 6 4 as as IN cord-014901-d9szap94 6 5 an an DT cord-014901-d9szap94 6 6 alternative alternative NN cord-014901-d9szap94 6 7 of of IN cord-014901-d9szap94 6 8 costly costly JJ cord-014901-d9szap94 6 9 systems system NNS cord-014901-d9szap94 6 10 of of IN cord-014901-d9szap94 6 11 recombinant recombinant JJ cord-014901-d9szap94 6 12 immunogenic immunogenic JJ cord-014901-d9szap94 6 13 protein protein NN cord-014901-d9szap94 6 14 expression expression NN cord-014901-d9szap94 6 15 are be VBP cord-014901-d9szap94 6 16 the the DT cord-014901-d9szap94 6 17 source source NN cord-014901-d9szap94 6 18 for for IN cord-014901-d9szap94 6 19 the the DT cord-014901-d9szap94 6 20 production production NN cord-014901-d9szap94 6 21 of of IN cord-014901-d9szap94 6 22 cheap cheap JJ cord-014901-d9szap94 6 23 and and CC cord-014901-d9szap94 6 24 highly highly RB cord-014901-d9szap94 6 25 efficient efficient JJ cord-014901-d9szap94 6 26 biotherapeuticals biotherapeutical NNS cord-014901-d9szap94 6 27 of of IN cord-014901-d9szap94 6 28 new new JJ cord-014901-d9szap94 6 29 generation generation NN cord-014901-d9szap94 6 30 , , , cord-014901-d9szap94 6 31 including include VBG cord-014901-d9szap94 6 32 plant plant NN cord-014901-d9szap94 6 33 vaccines vaccine NNS cord-014901-d9szap94 6 34 . . . cord-014901-d9szap94 7 1 In in IN cord-014901-d9szap94 7 2 the the DT cord-014901-d9szap94 7 3 present present JJ cord-014901-d9szap94 7 4 review review NN cord-014901-d9szap94 7 5 , , , cord-014901-d9szap94 7 6 possibilities possibility NNS cord-014901-d9szap94 7 7 of of IN cord-014901-d9szap94 7 8 plant plant NN cord-014901-d9szap94 7 9 system system NN cord-014901-d9szap94 7 10 application application NN cord-014901-d9szap94 7 11 for for IN cord-014901-d9szap94 7 12 the the DT cord-014901-d9szap94 7 13 production production NN cord-014901-d9szap94 7 14 of of IN cord-014901-d9szap94 7 15 recombinant recombinant JJ cord-014901-d9szap94 7 16 proteins protein NNS cord-014901-d9szap94 7 17 for for IN cord-014901-d9szap94 7 18 veterinary veterinary JJ cord-014901-d9szap94 7 19 use use NN cord-014901-d9szap94 7 20 are be VBP cord-014901-d9szap94 7 21 considered consider VBN cord-014901-d9szap94 7 22 , , , cord-014901-d9szap94 7 23 the the DT cord-014901-d9szap94 7 24 history history NN cord-014901-d9szap94 7 25 of of IN cord-014901-d9szap94 7 26 the the DT cord-014901-d9szap94 7 27 “ " `` cord-014901-d9szap94 7 28 edible edible JJ cord-014901-d9szap94 7 29 vaccine vaccine NN cord-014901-d9szap94 7 30 ” " '' cord-014901-d9szap94 7 31 concept concept NN cord-014901-d9szap94 7 32 is be VBZ cord-014901-d9szap94 7 33 briefly briefly RB cord-014901-d9szap94 7 34 summarized summarize VBN cord-014901-d9szap94 7 35 , , , cord-014901-d9szap94 7 36 advantages advantage NNS cord-014901-d9szap94 7 37 and and CC cord-014901-d9szap94 7 38 disadvantages disadvantage NNS cord-014901-d9szap94 7 39 of of IN cord-014901-d9szap94 7 40 various various JJ cord-014901-d9szap94 7 41 plant plant NN cord-014901-d9szap94 7 42 systems system NNS cord-014901-d9szap94 7 43 for for IN cord-014901-d9szap94 7 44 the the DT cord-014901-d9szap94 7 45 expression expression NN cord-014901-d9szap94 7 46 of of IN cord-014901-d9szap94 7 47 recombinant recombinant JJ cord-014901-d9szap94 7 48 immunogenic immunogenic JJ cord-014901-d9szap94 7 49 proteins protein NNS cord-014901-d9szap94 7 50 are be VBP cord-014901-d9szap94 7 51 discussed discuss VBN cord-014901-d9szap94 7 52 . . . cord-014901-d9szap94 8 1 The the DT cord-014901-d9szap94 8 2 list list NN cord-014901-d9szap94 8 3 of of IN cord-014901-d9szap94 8 4 recombinant recombinant JJ cord-014901-d9szap94 8 5 plant plant NN cord-014901-d9szap94 8 6 vaccines vaccine NNS cord-014901-d9szap94 8 7 for for IN cord-014901-d9szap94 8 8 veterinary veterinary JJ cord-014901-d9szap94 8 9 use use NN cord-014901-d9szap94 8 10 , , , cord-014901-d9szap94 8 11 which which WDT cord-014901-d9szap94 8 12 are be VBP cord-014901-d9szap94 8 13 at at IN cord-014901-d9szap94 8 14 different different JJ cord-014901-d9szap94 8 15 stages stage NNS cord-014901-d9szap94 8 16 of of IN cord-014901-d9szap94 8 17 clinical clinical JJ cord-014901-d9szap94 8 18 trials trial NNS cord-014901-d9szap94 8 19 , , , cord-014901-d9szap94 8 20 is be VBZ cord-014901-d9szap94 8 21 presented present VBN cord-014901-d9szap94 8 22 . . . cord-014901-d9szap94 9 1 For for IN cord-014901-d9szap94 9 2 many many JJ cord-014901-d9szap94 9 3 thousands thousand NNS cord-014901-d9szap94 9 4 of of IN cord-014901-d9szap94 9 5 years year NNS cord-014901-d9szap94 9 6 plants plant NNS cord-014901-d9szap94 9 7 have have VBP cord-014901-d9szap94 9 8 served serve VBN cord-014901-d9szap94 9 9 humanity humanity NN cord-014901-d9szap94 9 10 as as IN cord-014901-d9szap94 9 11 a a DT cord-014901-d9szap94 9 12 source source NN cord-014901-d9szap94 9 13 of of IN cord-014901-d9szap94 9 14 medicinal medicinal JJ cord-014901-d9szap94 9 15 substances substance NNS cord-014901-d9szap94 9 16 . . . cord-014901-d9szap94 10 1 How how WRB cord-014901-d9szap94 10 2 ever ever RB cord-014901-d9szap94 10 3 , , , cord-014901-d9szap94 10 4 only only RB cord-014901-d9szap94 10 5 at at IN cord-014901-d9szap94 10 6 the the DT cord-014901-d9szap94 10 7 turn turn NN cord-014901-d9szap94 10 8 of of IN cord-014901-d9szap94 10 9 the the DT cord-014901-d9szap94 10 10 century century NN cord-014901-d9szap94 10 11 XXI xxi NN cord-014901-d9szap94 10 12 with with IN cord-014901-d9szap94 10 13 the the DT cord-014901-d9szap94 10 14 appearance appearance NN cord-014901-d9szap94 10 15 of of IN cord-014901-d9szap94 10 16 DNA DNA NNP cord-014901-d9szap94 10 17 technologies technology NNS cord-014901-d9szap94 10 18 , , , cord-014901-d9szap94 10 19 it -PRON- PRP cord-014901-d9szap94 10 20 became become VBD cord-014901-d9szap94 10 21 possible possible JJ cord-014901-d9szap94 10 22 to to TO cord-014901-d9szap94 10 23 modify modify VB cord-014901-d9szap94 10 24 plant plant NN cord-014901-d9szap94 10 25 genomes genome NNS cord-014901-d9szap94 10 26 and and CC cord-014901-d9szap94 10 27 to to TO cord-014901-d9szap94 10 28 create create VB cord-014901-d9szap94 10 29 new new JJ cord-014901-d9szap94 10 30 types type NNS cord-014901-d9szap94 10 31 of of IN cord-014901-d9szap94 10 32 plants plant NNS cord-014901-d9szap94 10 33 ( ( -LRB- cord-014901-d9szap94 10 34 transgenic transgenic JJ cord-014901-d9szap94 10 35 plants plant NNS cord-014901-d9szap94 10 36 ) ) -RRB- cord-014901-d9szap94 10 37 , , , cord-014901-d9szap94 10 38 which which WDT cord-014901-d9szap94 10 39 are be VBP cord-014901-d9szap94 10 40 capable capable JJ cord-014901-d9szap94 10 41 of of IN cord-014901-d9szap94 10 42 syn syn NN cord-014901-d9szap94 10 43 thesizing thesizing NN cord-014901-d9szap94 10 44 and and CC cord-014901-d9szap94 10 45 accumulating accumulate VBG cord-014901-d9szap94 10 46 in in IN cord-014901-d9szap94 10 47 their -PRON- PRP$ cord-014901-d9szap94 10 48 tissues tissue NNS cord-014901-d9szap94 10 49 recombi recombi IN cord-014901-d9szap94 10 50 nant nant NNP cord-014901-d9szap94 10 51 proteins protein NNS cord-014901-d9szap94 10 52 from from IN cord-014901-d9szap94 10 53 various various JJ cord-014901-d9szap94 10 54 heterologous heterologous JJ cord-014901-d9szap94 10 55 systems system NNS cord-014901-d9szap94 10 56 . . . cord-014901-d9szap94 11 1 To to IN cord-014901-d9szap94 11 2 date date NN cord-014901-d9szap94 11 3 transgenic transgenic JJ cord-014901-d9szap94 11 4 plants plant NNS cord-014901-d9szap94 11 5 in in IN cord-014901-d9szap94 11 6 which which WDT cord-014901-d9szap94 11 7 nuclear nuclear JJ cord-014901-d9szap94 11 8 and and CC cord-014901-d9szap94 11 9 chloro chloro NN cord-014901-d9szap94 11 10 plast plast NN cord-014901-d9szap94 11 11 genomes genome NNS cord-014901-d9szap94 11 12 have have VBP cord-014901-d9szap94 11 13 been be VBN cord-014901-d9szap94 11 14 transformed transform VBN cord-014901-d9szap94 11 15 with with IN cord-014901-d9szap94 11 16 genes gene NNS cord-014901-d9szap94 11 17 encoding encode VBG cord-014901-d9szap94 11 18 heterologous heterologous NNP cord-014901-d9szap94 11 19 proteins protein NNS cord-014901-d9szap94 11 20 that that WDT cord-014901-d9szap94 11 21 are be VBP cord-014901-d9szap94 11 22 important important JJ cord-014901-d9szap94 11 23 in in IN cord-014901-d9szap94 11 24 the the DT cord-014901-d9szap94 11 25 treatment treatment NN cord-014901-d9szap94 11 26 of of IN cord-014901-d9szap94 11 27 various various JJ cord-014901-d9szap94 11 28 diseases disease NNS cord-014901-d9szap94 11 29 -antigens -antigens : cord-014901-d9szap94 11 30 of of IN cord-014901-d9szap94 11 31 infec infec IN cord-014901-d9szap94 11 32 tious tious JJ cord-014901-d9szap94 11 33 agents agent NNS cord-014901-d9szap94 11 34 , , , cord-014901-d9szap94 11 35 antibodies antibody NNS cord-014901-d9szap94 11 36 , , , cord-014901-d9szap94 11 37 immunomodulators immunomodulator NNS cord-014901-d9szap94 11 38 , , , cord-014901-d9szap94 11 39 etc etc FW cord-014901-d9szap94 11 40 . . . cord-014901-d9szap94 12 1 have have VBP cord-014901-d9szap94 12 2 been be VBN cord-014901-d9szap94 12 3 created create VBN cord-014901-d9szap94 12 4 [ [ -LRB- cord-014901-d9szap94 12 5 1 1 CD cord-014901-d9szap94 12 6 , , , cord-014901-d9szap94 12 7 2 2 CD cord-014901-d9szap94 12 8 ] ] -RRB- cord-014901-d9szap94 12 9 . . . cord-014901-d9szap94 13 1 Of of IN cord-014901-d9szap94 13 2 principal principal JJ cord-014901-d9szap94 13 3 importance importance NN cord-014901-d9szap94 13 4 of of IN cord-014901-d9szap94 13 5 this this DT cord-014901-d9szap94 13 6 work work NN cord-014901-d9szap94 13 7 development development NN cord-014901-d9szap94 13 8 was be VBD cord-014901-d9szap94 13 9 the the DT cord-014901-d9szap94 13 10 creation creation NN cord-014901-d9szap94 13 11 of of IN cord-014901-d9szap94 13 12 the the DT cord-014901-d9szap94 13 13 " " `` cord-014901-d9szap94 13 14 edible edible JJ cord-014901-d9szap94 13 15 vaccine vaccine NN cord-014901-d9szap94 13 16 " " '' cord-014901-d9szap94 13 17 concept concept NN cord-014901-d9szap94 13 18 , , , cord-014901-d9szap94 13 19 the the DT cord-014901-d9szap94 13 20 essence essence NN cord-014901-d9szap94 13 21 of of IN cord-014901-d9szap94 13 22 which which WDT cord-014901-d9szap94 13 23 is be VBZ cord-014901-d9szap94 13 24 the the DT cord-014901-d9szap94 13 25 use use NN cord-014901-d9szap94 13 26 of of IN cord-014901-d9szap94 13 27 genetically genetically RB cord-014901-d9szap94 13 28 modified modify VBN cord-014901-d9szap94 13 29 plants plant NNS cord-014901-d9szap94 13 30 containing contain VBG cord-014901-d9szap94 13 31 protein protein NN cord-014901-d9szap94 13 32 anti anti JJ cord-014901-d9szap94 13 33 gens gen NNS cord-014901-d9szap94 13 34 of of IN cord-014901-d9szap94 13 35 infectious infectious JJ cord-014901-d9szap94 13 36 agents agent NNS cord-014901-d9szap94 13 37 for for IN cord-014901-d9szap94 13 38 oral oral JJ cord-014901-d9szap94 13 39 delivery delivery NN cord-014901-d9szap94 13 40 of of IN cord-014901-d9szap94 13 41 relevant relevant JJ cord-014901-d9szap94 13 42 antigens antigen NNS cord-014901-d9szap94 13 43 to to IN cord-014901-d9szap94 13 44 the the DT cord-014901-d9szap94 13 45 mucosa mucosa NN cord-014901-d9szap94 13 46 of of IN cord-014901-d9szap94 13 47 the the DT cord-014901-d9szap94 13 48 gastrointestinal gastrointestinal JJ cord-014901-d9szap94 13 49 tract tract NN cord-014901-d9szap94 13 50 of of IN cord-014901-d9szap94 13 51 warm warm JJ cord-014901-d9szap94 13 52 blooded blooded JJ cord-014901-d9szap94 13 53 animals animal NNS cord-014901-d9szap94 13 54 . . . cord-014901-d9szap94 14 1 Vaccination vaccination NN cord-014901-d9szap94 14 2 based base VBN cord-014901-d9szap94 14 3 on on IN cord-014901-d9szap94 14 4 the the DT cord-014901-d9szap94 14 5 programming programming NN cord-014901-d9szap94 14 6 of of IN cord-014901-d9szap94 14 7 the the DT cord-014901-d9szap94 14 8 spe spe NNP cord-014901-d9szap94 14 9 cific cific NNP cord-014901-d9szap94 14 10 mechanisms mechanism NNS cord-014901-d9szap94 14 11 of of IN cord-014901-d9szap94 14 12 warm warm JJ cord-014901-d9szap94 14 13 blooded blooded JJ cord-014901-d9szap94 14 14 animal animal NN cord-014901-d9szap94 14 15 protection protection NN cord-014901-d9szap94 14 16 against against IN cord-014901-d9szap94 14 17 pathogens pathogen NNS cord-014901-d9szap94 14 18 is be VBZ cord-014901-d9szap94 14 19 the the DT cord-014901-d9szap94 14 20 most most RBS cord-014901-d9szap94 14 21 efficient efficient JJ cord-014901-d9szap94 14 22 method method NN cord-014901-d9szap94 14 23 for for IN cord-014901-d9szap94 14 24 the the DT cord-014901-d9szap94 14 25 struggle struggle NN cord-014901-d9szap94 14 26 against against IN cord-014901-d9szap94 14 27 infectious infectious JJ cord-014901-d9szap94 14 28 diseases disease NNS cord-014901-d9szap94 14 29 , , , cord-014901-d9szap94 14 30 which which WDT cord-014901-d9szap94 14 31 often often RB cord-014901-d9szap94 14 32 result result VBP cord-014901-d9szap94 14 33 in in IN cord-014901-d9szap94 14 34 a a DT cord-014901-d9szap94 14 35 mass mass NN cord-014901-d9szap94 14 36 mortality mortality NN cord-014901-d9szap94 14 37 . . . cord-014901-d9szap94 15 1 In in IN cord-014901-d9szap94 15 2 agriculture agriculture NN cord-014901-d9szap94 15 3 , , , cord-014901-d9szap94 15 4 there there EX cord-014901-d9szap94 15 5 is be VBZ cord-014901-d9szap94 15 6 no no DT cord-014901-d9szap94 15 7 alterna alterna FW cord-014901-d9szap94 15 8 tive tive JJ cord-014901-d9szap94 15 9 to to IN cord-014901-d9szap94 15 10 livestock livestock NN cord-014901-d9szap94 15 11 vaccination vaccination NN cord-014901-d9szap94 15 12 , , , cord-014901-d9szap94 15 13 because because IN cord-014901-d9szap94 15 14 there there EX cord-014901-d9szap94 15 15 are be VBP cord-014901-d9szap94 15 16 no no DT cord-014901-d9szap94 15 17 anti anti JJ cord-014901-d9szap94 15 18 viral viral JJ cord-014901-d9szap94 15 19 drugs drug NNS cord-014901-d9szap94 15 20 that that WDT cord-014901-d9szap94 15 21 are be VBP cord-014901-d9szap94 15 22 suitable suitable JJ cord-014901-d9szap94 15 23 for for IN cord-014901-d9szap94 15 24 a a DT cord-014901-d9szap94 15 25 wide wide JJ cord-014901-d9szap94 15 26 use use NN cord-014901-d9szap94 15 27 in in IN cord-014901-d9szap94 15 28 animal animal NN cord-014901-d9szap94 15 29 husbandry husbandry NN cord-014901-d9szap94 15 30 . . . cord-014901-d9szap94 16 1 The the DT cord-014901-d9szap94 16 2 importance importance NN cord-014901-d9szap94 16 3 of of IN cord-014901-d9szap94 16 4 animal animal NN cord-014901-d9szap94 16 5 vaccination vaccination NN cord-014901-d9szap94 16 6 indirectly indirectly RB cord-014901-d9szap94 16 7 affects affect VBZ cord-014901-d9szap94 16 8 human human JJ cord-014901-d9szap94 16 9 health health NN cord-014901-d9szap94 16 10 , , , cord-014901-d9szap94 16 11 because because IN cord-014901-d9szap94 16 12 the the DT cord-014901-d9szap94 16 13 use use NN cord-014901-d9szap94 16 14 of of IN cord-014901-d9szap94 16 15 vaccines vaccine NNS cord-014901-d9szap94 16 16 significantly significantly RB cord-014901-d9szap94 16 17 reduces reduce VBZ cord-014901-d9szap94 16 18 the the DT cord-014901-d9szap94 16 19 amount amount NN cord-014901-d9szap94 16 20 of of IN cord-014901-d9szap94 16 21 pharma pharma NNS cord-014901-d9szap94 16 22 ceuticals ceutical NNS cord-014901-d9szap94 16 23 in in IN cord-014901-d9szap94 16 24 the the DT cord-014901-d9szap94 16 25 food food NN cord-014901-d9szap94 16 26 chain chain NN cord-014901-d9szap94 16 27 . . . cord-014901-d9szap94 17 1 As as IN cord-014901-d9szap94 17 2 a a DT cord-014901-d9szap94 17 3 rule rule NN cord-014901-d9szap94 17 4 , , , cord-014901-d9szap94 17 5 animal animal NN cord-014901-d9szap94 17 6 immune immune JJ cord-014901-d9szap94 17 7 mechanisms mechanism NNS cord-014901-d9szap94 17 8 are be VBP cord-014901-d9szap94 17 9 acti acti NNP cord-014901-d9szap94 17 10 vated vate VBN cord-014901-d9szap94 17 11 by by IN cord-014901-d9szap94 17 12 the the DT cord-014901-d9szap94 17 13 direct direct JJ cord-014901-d9szap94 17 14 introduction introduction NN cord-014901-d9szap94 17 15 of of IN cord-014901-d9szap94 17 16 infectious infectious JJ cord-014901-d9szap94 17 17 agents agent NNS cord-014901-d9szap94 17 18 or or CC cord-014901-d9szap94 17 19 their -PRON- PRP$ cord-014901-d9szap94 17 20 components component NNS cord-014901-d9szap94 17 21 . . . cord-014901-d9szap94 18 1 At at IN cord-014901-d9szap94 18 2 present present JJ cord-014901-d9szap94 18 3 , , , cord-014901-d9szap94 18 4 most most JJS cord-014901-d9szap94 18 5 of of IN cord-014901-d9szap94 18 6 used use VBN cord-014901-d9szap94 18 7 vaccines vaccine NNS cord-014901-d9szap94 18 8 are be VBP cord-014901-d9szap94 18 9 preparations preparation NNS cord-014901-d9szap94 18 10 on on IN cord-014901-d9szap94 18 11 the the DT cord-014901-d9szap94 18 12 basis basis NN cord-014901-d9szap94 18 13 of of IN cord-014901-d9szap94 18 14 inactivated inactivate VBN cord-014901-d9szap94 18 15 agents agent NNS cord-014901-d9szap94 18 16 . . . cord-014901-d9szap94 19 1 Although although IN cord-014901-d9szap94 19 2 these these DT cord-014901-d9szap94 19 3 vaccines vaccine NNS cord-014901-d9szap94 19 4 manifest manifest VBP cord-014901-d9szap94 19 5 the the DT cord-014901-d9szap94 19 6 high high JJ cord-014901-d9szap94 19 7 immunoge immunoge NNP cord-014901-d9szap94 19 8 nicity nicity NNP cord-014901-d9szap94 19 9 , , , cord-014901-d9szap94 19 10 they -PRON- PRP cord-014901-d9szap94 19 11 are be VBP cord-014901-d9szap94 19 12 not not RB cord-014901-d9szap94 19 13 without without IN cord-014901-d9szap94 19 14 serious serious JJ cord-014901-d9szap94 19 15 shortcomings shortcoming NNS cord-014901-d9szap94 19 16 . . . cord-014901-d9szap94 20 1 Among among IN cord-014901-d9szap94 20 2 such such JJ cord-014901-d9szap94 20 3 disadvantages disadvantage NNS cord-014901-d9szap94 20 4 are be VBP cord-014901-d9szap94 20 5 the the DT cord-014901-d9szap94 20 6 increased increase VBN cord-014901-d9szap94 20 7 sensitiv sensitiv NNP cord-014901-d9szap94 20 8 ity ity NNP cord-014901-d9szap94 20 9 of of IN cord-014901-d9szap94 20 10 the the DT cord-014901-d9szap94 20 11 organism organism NN cord-014901-d9szap94 20 12 to to IN cord-014901-d9szap94 20 13 them -PRON- PRP cord-014901-d9szap94 20 14 , , , cord-014901-d9szap94 20 15 the the DT cord-014901-d9szap94 20 16 large large JJ cord-014901-d9szap94 20 17 load load NN cord-014901-d9szap94 20 18 on on IN cord-014901-d9szap94 20 19 the the DT cord-014901-d9szap94 20 20 immune immune JJ cord-014901-d9szap94 20 21 system system NN cord-014901-d9szap94 20 22 , , , cord-014901-d9szap94 20 23 the the DT cord-014901-d9szap94 20 24 reactogenicity reactogenicity NN cord-014901-d9szap94 20 25 of of IN cord-014901-d9szap94 20 26 vaccines vaccine NNS cord-014901-d9szap94 20 27 ( ( -LRB- cord-014901-d9szap94 20 28 side side NN cord-014901-d9szap94 20 29 effects effect NNS cord-014901-d9szap94 20 30 ) ) -RRB- cord-014901-d9szap94 20 31 , , , cord-014901-d9szap94 20 32 their -PRON- PRP$ cord-014901-d9szap94 20 33 toxicity toxicity NN cord-014901-d9szap94 20 34 . . . cord-014901-d9szap94 21 1 etc etc FW cord-014901-d9szap94 21 2 . . . cord-014901-d9szap94 22 1 The the DT cord-014901-d9szap94 22 2 application application NN cord-014901-d9szap94 22 3 of of IN cord-014901-d9szap94 22 4 molecular molecular JJ cord-014901-d9szap94 22 5 biology biology NN cord-014901-d9szap94 22 6 and and CC cord-014901-d9szap94 22 7 genetic genetic JJ cord-014901-d9szap94 22 8 engineering engineering NN cord-014901-d9szap94 22 9 methods method NNS cord-014901-d9szap94 22 10 opened open VBD cord-014901-d9szap94 22 11 wide wide JJ cord-014901-d9szap94 22 12 prospects prospect NNS cord-014901-d9szap94 22 13 for for IN cord-014901-d9szap94 22 14 the the DT cord-014901-d9szap94 22 15 manufacturing manufacturing NN cord-014901-d9szap94 22 16 of of IN cord-014901-d9szap94 22 17 vaccines vaccine NNS cord-014901-d9szap94 22 18 of of IN cord-014901-d9szap94 22 19 new new JJ cord-014901-d9szap94 22 20 generation generation NN cord-014901-d9szap94 22 21 , , , cord-014901-d9szap94 22 22 which which WDT cord-014901-d9szap94 22 23 immunogenic immunogenic JJ cord-014901-d9szap94 22 24 components component NNS cord-014901-d9szap94 22 25 can can MD cord-014901-d9szap94 22 26 be be VB cord-014901-d9szap94 22 27 biological biological JJ cord-014901-d9szap94 22 28 mole mole NN cord-014901-d9szap94 22 29 cules cule NNS cord-014901-d9szap94 22 30 or or CC cord-014901-d9szap94 22 31 their -PRON- PRP$ cord-014901-d9szap94 22 32 fragments fragment NNS cord-014901-d9szap94 22 33 . . . cord-014901-d9szap94 23 1 DNA dna NN cord-014901-d9szap94 23 2 fragments fragment NNS cord-014901-d9szap94 23 3 or or CC cord-014901-d9szap94 23 4 proteins protein NNS cord-014901-d9szap94 23 5 of of IN cord-014901-d9szap94 23 6 the the DT cord-014901-d9szap94 23 7 infectious infectious JJ cord-014901-d9szap94 23 8 agent agent NN cord-014901-d9szap94 23 9 cell cell NN cord-014901-d9szap94 23 10 envelopes envelope NNS cord-014901-d9szap94 23 11 can can MD cord-014901-d9szap94 23 12 serve serve VB cord-014901-d9szap94 23 13 as as IN cord-014901-d9szap94 23 14 immu immu NNP cord-014901-d9szap94 23 15 nogenic nogenic NNP cord-014901-d9szap94 23 16 components component NNS cord-014901-d9szap94 23 17 . . . cord-014901-d9szap94 24 1 When when WRB cord-014901-d9szap94 24 2 the the DT cord-014901-d9szap94 24 3 gene gene NN cord-014901-d9szap94 24 4 encoding encode VBG cord-014901-d9szap94 24 5 the the DT cord-014901-d9szap94 24 6 envelope envelope NN cord-014901-d9szap94 24 7 protein protein NN cord-014901-d9szap94 24 8 of of IN cord-014901-d9szap94 24 9 the the DT cord-014901-d9szap94 24 10 infectious infectious JJ cord-014901-d9szap94 24 11 agent agent NN cord-014901-d9szap94 24 12 is be VBZ cord-014901-d9szap94 24 13 transferred transfer VBN cord-014901-d9szap94 24 14 into into IN cord-014901-d9szap94 24 15 the the DT cord-014901-d9szap94 24 16 genome genome NN cord-014901-d9szap94 24 17 of of IN cord-014901-d9szap94 24 18 another another DT cord-014901-d9szap94 24 19 organism organism NN cord-014901-d9szap94 24 20 , , , cord-014901-d9szap94 24 21 for for IN cord-014901-d9szap94 24 22 example example NN cord-014901-d9szap94 24 23 , , , cord-014901-d9szap94 24 24 plant plant NN cord-014901-d9szap94 24 25 , , , cord-014901-d9szap94 24 26 then then RB cord-014901-d9szap94 24 27 the the DT cord-014901-d9szap94 24 28 cells cell NNS cord-014901-d9szap94 24 29 of of IN cord-014901-d9szap94 24 30 such such JJ cord-014901-d9szap94 24 31 plant plant NN cord-014901-d9szap94 24 32 will will MD cord-014901-d9szap94 24 33 synthesize synthesize VB cord-014901-d9szap94 24 34 the the DT cord-014901-d9szap94 24 35 protein protein NN cord-014901-d9szap94 24 36 antigen antigen NN cord-014901-d9szap94 24 37 capable capable JJ cord-014901-d9szap94 24 38 of of IN cord-014901-d9szap94 24 39 formation formation NN cord-014901-d9szap94 24 40 of of IN cord-014901-d9szap94 24 41 resistance resistance NN cord-014901-d9szap94 24 42 to to IN cord-014901-d9szap94 24 43 this this DT cord-014901-d9szap94 24 44 agent agent NN cord-014901-d9szap94 24 45 . . . cord-014901-d9szap94 25 1 Thus thus RB cord-014901-d9szap94 25 2 , , , cord-014901-d9szap94 25 3 the the DT cord-014901-d9szap94 25 4 introduction introduction NN cord-014901-d9szap94 25 5 into into IN cord-014901-d9szap94 25 6 the the DT cord-014901-d9szap94 25 7 organism organism NN cord-014901-d9szap94 25 8 not not RB cord-014901-d9szap94 25 9 the the DT cord-014901-d9szap94 25 10 whole whole JJ cord-014901-d9szap94 25 11 pathogen pathogen NN cord-014901-d9szap94 25 12 but but CC cord-014901-d9szap94 25 13 only only RB cord-014901-d9szap94 25 14 its -PRON- PRP$ cord-014901-d9szap94 25 15 part part NN cord-014901-d9szap94 25 16 , , , cord-014901-d9szap94 25 17 which which WDT cord-014901-d9szap94 25 18 is be VBZ cord-014901-d9szap94 25 19 not not RB cord-014901-d9szap94 25 20 capable capable JJ cord-014901-d9szap94 25 21 of of IN cord-014901-d9szap94 25 22 inducing induce VBG cord-014901-d9szap94 25 23 infection infection NN cord-014901-d9szap94 25 24 development development NN cord-014901-d9szap94 25 25 , , , cord-014901-d9szap94 25 26 will will MD cord-014901-d9szap94 25 27 pro pro VB cord-014901-d9szap94 25 28 vide vide NN cord-014901-d9szap94 25 29 for for IN cord-014901-d9szap94 25 30 the the DT cord-014901-d9szap94 25 31 effect effect NN cord-014901-d9szap94 25 32 of of IN cord-014901-d9szap94 25 33 vaccination vaccination NN cord-014901-d9szap94 25 34 . . . cord-014901-d9szap94 26 1 one one CD cord-014901-d9szap94 26 2 fourth fourth NN cord-014901-d9szap94 26 3 of of IN cord-014901-d9szap94 26 4 the the DT cord-014901-d9szap94 26 5 total total JJ cord-014901-d9szap94 26 6 pharmaceutical pharmaceutical JJ cord-014901-d9szap94 26 7 market market NN cord-014901-d9szap94 26 8 of of IN cord-014901-d9szap94 26 9 drugs drug NNS cord-014901-d9szap94 26 10 for for IN cord-014901-d9szap94 26 11 veterinary veterinary JJ cord-014901-d9szap94 26 12 use use NN cord-014901-d9szap94 26 13 , , , cord-014901-d9szap94 26 14 and and CC cord-014901-d9szap94 26 15 it -PRON- PRP cord-014901-d9szap94 26 16 is be VBZ cord-014901-d9szap94 26 17 constantly constantly RB cord-014901-d9szap94 26 18 expanding expand VBG cord-014901-d9szap94 26 19 . . . cord-014901-d9szap94 27 1 [ [ -LRB- cord-014901-d9szap94 27 2 3 3 CD cord-014901-d9szap94 27 3 ] ] -RRB- cord-014901-d9szap94 27 4 . . . cord-014901-d9szap94 28 1 Preparation preparation NN cord-014901-d9szap94 28 2 of of IN cord-014901-d9szap94 28 3 medicinal medicinal JJ cord-014901-d9szap94 28 4 substances substance NNS cord-014901-d9szap94 28 5 for for IN cord-014901-d9szap94 28 6 the the DT cord-014901-d9szap94 28 7 pro pro JJ cord-014901-d9szap94 28 8 duction duction NN cord-014901-d9szap94 28 9 of of IN cord-014901-d9szap94 28 10 veterinary veterinary JJ cord-014901-d9szap94 28 11 products product NNS cord-014901-d9szap94 28 12 is be VBZ cord-014901-d9szap94 28 13 based base VBN cord-014901-d9szap94 28 14 on on IN cord-014901-d9szap94 28 15 various various JJ cord-014901-d9szap94 28 16 approaches approach NNS cord-014901-d9szap94 28 17 , , , cord-014901-d9szap94 28 18 including include VBG cord-014901-d9szap94 28 19 biotechnology biotechnology NN cord-014901-d9szap94 28 20 using use VBG cord-014901-d9szap94 28 21 genetically genetically RB cord-014901-d9szap94 28 22 modified modify VBN cord-014901-d9szap94 28 23 ( ( -LRB- cord-014901-d9szap94 28 24 transgenic transgenic JJ cord-014901-d9szap94 28 25 ) ) -RRB- cord-014901-d9szap94 28 26 organisms organism NNS cord-014901-d9szap94 28 27 for for IN cord-014901-d9szap94 28 28 these these DT cord-014901-d9szap94 28 29 purposes purpose NNS cord-014901-d9szap94 28 30 ; ; , cord-014901-d9szap94 28 31 such such JJ cord-014901-d9szap94 28 32 expression expression NN cord-014901-d9szap94 28 33 systems system NNS cord-014901-d9szap94 28 34 as as IN cord-014901-d9szap94 28 35 bacteria bacteria NNS cord-014901-d9szap94 28 36 , , , cord-014901-d9szap94 28 37 yeast yeast NN cord-014901-d9szap94 28 38 , , , cord-014901-d9szap94 28 39 cells cell NNS cord-014901-d9szap94 28 40 of of IN cord-014901-d9szap94 28 41 insects insect NNS cord-014901-d9szap94 28 42 and and CC cord-014901-d9szap94 28 43 mammals mammal NNS cord-014901-d9szap94 28 44 are be VBP cord-014901-d9szap94 28 45 used use VBN cord-014901-d9szap94 28 46 . . . cord-014901-d9szap94 29 1 The the DT cord-014901-d9szap94 29 2 application application NN cord-014901-d9szap94 29 3 of of IN cord-014901-d9szap94 29 4 genetically genetically RB cord-014901-d9szap94 29 5 modified modify VBN cord-014901-d9szap94 29 6 plants plant NNS cord-014901-d9szap94 29 7 with with IN cord-014901-d9szap94 29 8 genes gene NNS cord-014901-d9szap94 29 9 encoding encode VBG cord-014901-d9szap94 29 10 phar phar NNP cord-014901-d9szap94 29 11 maceutic maceutic NNP cord-014901-d9szap94 29 12 proteins protein NNS cord-014901-d9szap94 29 13 inserted insert VBN cord-014901-d9szap94 29 14 in in IN cord-014901-d9szap94 29 15 the the DT cord-014901-d9szap94 29 16 genome genome NN cord-014901-d9szap94 29 17 opens open VBZ cord-014901-d9szap94 29 18 new new JJ cord-014901-d9szap94 29 19 prospects prospect NNS cord-014901-d9szap94 29 20 for for IN cord-014901-d9szap94 29 21 obtaining obtain VBG cord-014901-d9szap94 29 22 recombinant recombinant JJ cord-014901-d9szap94 29 23 proteins protein NNS cord-014901-d9szap94 29 24 , , , cord-014901-d9szap94 29 25 includ includ NNS cord-014901-d9szap94 29 26 ing e VBG cord-014901-d9szap94 29 27 plant plant NN cord-014901-d9szap94 29 28 vaccines vaccine NNS cord-014901-d9szap94 29 29 [ [ -LRB- cord-014901-d9szap94 29 30 4 4 CD cord-014901-d9szap94 29 31 ] ] -RRB- cord-014901-d9szap94 29 32 . . . cord-014901-d9szap94 30 1 This this DT cord-014901-d9szap94 30 2 review review NN cord-014901-d9szap94 30 3 is be VBZ cord-014901-d9szap94 30 4 devoted devote VBN cord-014901-d9szap94 30 5 to to IN cord-014901-d9szap94 30 6 the the DT cord-014901-d9szap94 30 7 analysis analysis NN cord-014901-d9szap94 30 8 of of IN cord-014901-d9szap94 30 9 possibilities possibility NNS cord-014901-d9szap94 30 10 of of IN cord-014901-d9szap94 30 11 producing produce VBG cord-014901-d9szap94 30 12 recombinant recombinant JJ cord-014901-d9szap94 30 13 immunogenic immunogenic JJ cord-014901-d9szap94 30 14 proteins protein NNS cord-014901-d9szap94 30 15 for for IN cord-014901-d9szap94 30 16 veterinary veterinary JJ cord-014901-d9szap94 30 17 use use NN cord-014901-d9szap94 30 18 on on IN cord-014901-d9szap94 30 19 the the DT cord-014901-d9szap94 30 20 basis basis NN cord-014901-d9szap94 30 21 of of IN cord-014901-d9szap94 30 22 plant plant NN cord-014901-d9szap94 30 23 expression expression NN cord-014901-d9szap94 30 24 systems system NNS cord-014901-d9szap94 30 25 , , , cord-014901-d9szap94 30 26 and and CC cord-014901-d9szap94 30 27 the the DT cord-014901-d9szap94 30 28 history history NN cord-014901-d9szap94 30 29 of of IN cord-014901-d9szap94 30 30 the the DT cord-014901-d9szap94 30 31 concept concept NN cord-014901-d9szap94 30 32 of of IN cord-014901-d9szap94 30 33 " " `` cord-014901-d9szap94 30 34 edible edible JJ cord-014901-d9szap94 30 35 vaccines vaccine NNS cord-014901-d9szap94 30 36 " " '' cord-014901-d9szap94 30 37 for for IN cord-014901-d9szap94 30 38 animal animal NN cord-014901-d9szap94 30 39 immunization immunization NN cord-014901-d9szap94 30 40 . . . cord-014901-d9szap94 31 1 OF of IN cord-014901-d9szap94 31 2 PLANT plant NN cord-014901-d9szap94 31 3 VACCINES vaccine NNS cord-014901-d9szap94 32 1 The the DT cord-014901-d9szap94 32 2 idea idea NN cord-014901-d9szap94 32 3 of of IN cord-014901-d9szap94 32 4 the the DT cord-014901-d9szap94 32 5 usage usage NN cord-014901-d9szap94 32 6 of of IN cord-014901-d9szap94 32 7 plant plant NN cord-014901-d9szap94 32 8 cells cell NNS cord-014901-d9szap94 32 9 for for IN cord-014901-d9szap94 32 10 the the DT cord-014901-d9szap94 32 11 synthesis synthesis NN cord-014901-d9szap94 32 12 and and CC cord-014901-d9szap94 32 13 accumulation accumulation NN cord-014901-d9szap94 32 14 of of IN cord-014901-d9szap94 32 15 recombinant recombinant JJ cord-014901-d9szap94 32 16 protein protein NN cord-014901-d9szap94 32 17 antigens antigen NNS cord-014901-d9szap94 32 18 was be VBD cord-014901-d9szap94 32 19 for for IN cord-014901-d9szap94 32 20 the the DT cord-014901-d9szap94 32 21 first first JJ cord-014901-d9szap94 32 22 time time NN cord-014901-d9szap94 32 23 successfully successfully RB cord-014901-d9szap94 32 24 realized realize VBD cord-014901-d9szap94 32 25 in in IN cord-014901-d9szap94 32 26 1992 1992 CD cord-014901-d9szap94 32 27 by by IN cord-014901-d9szap94 32 28 C. C. NNP cord-014901-d9szap94 32 29 Arntzen Arntzen NNP cord-014901-d9szap94 32 30 and and CC cord-014901-d9szap94 32 31 his -PRON- PRP$ cord-014901-d9szap94 32 32 colleagues colleague NNS cord-014901-d9szap94 32 33 [ [ -LRB- cord-014901-d9szap94 32 34 5 5 CD cord-014901-d9szap94 32 35 ] ] -RRB- cord-014901-d9szap94 32 36 . . . cord-014901-d9szap94 33 1 Just just RB cord-014901-d9szap94 33 2 this this DT cord-014901-d9szap94 33 3 team team NN cord-014901-d9szap94 33 4 of of IN cord-014901-d9szap94 33 5 researchers researcher NNS cord-014901-d9szap94 33 6 not not RB cord-014901-d9szap94 33 7 only only RB cord-014901-d9szap94 33 8 demonstrated demonstrate VBD cord-014901-d9szap94 33 9 a a DT cord-014901-d9szap94 33 10 possibility possibility NN cord-014901-d9szap94 33 11 of of IN cord-014901-d9szap94 33 12 the the DT cord-014901-d9szap94 33 13 accumulation accumulation NN cord-014901-d9szap94 33 14 of of IN cord-014901-d9szap94 33 15 the the DT cord-014901-d9szap94 33 16 surface surface NN cord-014901-d9szap94 33 17 HBsAg hbsag ADD cord-014901-d9szap94 33 18 antigen antigen NN cord-014901-d9szap94 33 19 of of IN cord-014901-d9szap94 33 20 hepati hepati NNP cord-014901-d9szap94 33 21 tis tis NNP cord-014901-d9szap94 33 22 B B NNP cord-014901-d9szap94 33 23 virus virus NN cord-014901-d9szap94 33 24 but but CC cord-014901-d9szap94 33 25 also also RB cord-014901-d9szap94 33 26 its -PRON- PRP$ cord-014901-d9szap94 33 27 capability capability NN cord-014901-d9szap94 33 28 for for IN cord-014901-d9szap94 33 29 self self NN cord-014901-d9szap94 33 30 assembling assemble VBG cord-014901-d9szap94 33 31 in in IN cord-014901-d9szap94 33 32 the the DT cord-014901-d9szap94 33 33 virus virus NN cord-014901-d9szap94 33 34 like like IN cord-014901-d9szap94 33 35 particles particle NNS cord-014901-d9szap94 33 36 in in IN cord-014901-d9szap94 33 37 transgenic transgenic JJ cord-014901-d9szap94 33 38 tobacco tobacco NN cord-014901-d9szap94 33 39 plants plant NNS cord-014901-d9szap94 33 40 . . . cord-014901-d9szap94 34 1 The the DT cord-014901-d9szap94 34 2 virus virus NN cord-014901-d9szap94 34 3 like like IN cord-014901-d9szap94 34 4 particles particle NNS cord-014901-d9szap94 34 5 isolated isolate VBN cord-014901-d9szap94 34 6 from from IN cord-014901-d9szap94 34 7 plant plant NN cord-014901-d9szap94 34 8 tissues tissue NNS cord-014901-d9szap94 34 9 were be VBD cord-014901-d9szap94 34 10 identical identical JJ cord-014901-d9szap94 34 11 to to IN cord-014901-d9szap94 34 12 the the DT cord-014901-d9szap94 34 13 particles particle NNS cord-014901-d9szap94 34 14 of of IN cord-014901-d9szap94 34 15 HBsAg hbsag ADD cord-014901-d9szap94 34 16 antigen antigen NNP cord-014901-d9szap94 34 17 of of IN cord-014901-d9szap94 34 18 indus indus NNP cord-014901-d9szap94 34 19 trial trial NN cord-014901-d9szap94 34 20 recombinant recombinant JJ cord-014901-d9szap94 34 21 vaccine vaccine NN cord-014901-d9szap94 34 22 obtained obtain VBN cord-014901-d9szap94 34 23 in in IN cord-014901-d9szap94 34 24 the the DT cord-014901-d9szap94 34 25 yeast yeast NN cord-014901-d9szap94 34 26 expres expres NNP cord-014901-d9szap94 34 27 sion sion NNP cord-014901-d9szap94 34 28 system system NN cord-014901-d9szap94 34 29 and and CC cord-014901-d9szap94 34 30 also also RB cord-014901-d9szap94 34 31 to to IN cord-014901-d9szap94 34 32 virus virus NN cord-014901-d9szap94 34 33 like like IN cord-014901-d9szap94 34 34 particles particle NNS cord-014901-d9szap94 34 35 from from IN cord-014901-d9szap94 34 36 the the DT cord-014901-d9szap94 34 37 blood blood NN cord-014901-d9szap94 34 38 plasma plasma NN cord-014901-d9szap94 34 39 of of IN cord-014901-d9szap94 34 40 patients patient NNS cord-014901-d9szap94 34 41 infected infect VBN cord-014901-d9szap94 34 42 with with IN cord-014901-d9szap94 34 43 hepatitis hepatitis NN cord-014901-d9szap94 34 44 B B NNP cord-014901-d9szap94 34 45 virus virus NN cord-014901-d9szap94 34 46 . . . cord-014901-d9szap94 35 1 Thus thus RB cord-014901-d9szap94 35 2 , , , cord-014901-d9szap94 35 3 it -PRON- PRP cord-014901-d9szap94 35 4 became become VBD cord-014901-d9szap94 35 5 obvious obvious JJ cord-014901-d9szap94 35 6 that that IN cord-014901-d9szap94 35 7 genetically genetically RB cord-014901-d9szap94 35 8 modi modi NNP cord-014901-d9szap94 35 9 fied fie VBN cord-014901-d9szap94 35 10 plants plant NNS cord-014901-d9szap94 35 11 producing produce VBG cord-014901-d9szap94 35 12 and and CC cord-014901-d9szap94 35 13 accumulating accumulate VBG cord-014901-d9szap94 35 14 protein protein NN cord-014901-d9szap94 35 15 anti anti JJ cord-014901-d9szap94 35 16 gens gen NNS cord-014901-d9szap94 35 17 of of IN cord-014901-d9szap94 35 18 various various JJ cord-014901-d9szap94 35 19 infectious infectious JJ cord-014901-d9szap94 35 20 agents agent NNS cord-014901-d9szap94 35 21 can can MD cord-014901-d9szap94 35 22 be be VB cord-014901-d9szap94 35 23 used use VBN cord-014901-d9szap94 35 24 for for IN cord-014901-d9szap94 35 25 oral oral JJ cord-014901-d9szap94 35 26 delivery delivery NN cord-014901-d9szap94 35 27 of of IN cord-014901-d9szap94 35 28 corresponding correspond VBG cord-014901-d9szap94 35 29 agents agent NNS cord-014901-d9szap94 35 30 to to IN cord-014901-d9szap94 35 31 the the DT cord-014901-d9szap94 35 32 mucosa mucosa NN cord-014901-d9szap94 35 33 of of IN cord-014901-d9szap94 35 34 the the DT cord-014901-d9szap94 35 35 gastrointestinal gastrointestinal JJ cord-014901-d9szap94 35 36 tract tract NN cord-014901-d9szap94 35 37 of of IN cord-014901-d9szap94 35 38 warm warm JJ cord-014901-d9szap94 35 39 blooded blooded JJ cord-014901-d9szap94 35 40 animals animal NNS cord-014901-d9szap94 35 41 , , , cord-014901-d9szap94 35 42 i.e. i.e. FW cord-014901-d9szap94 35 43 , , , cord-014901-d9szap94 35 44 as as IN cord-014901-d9szap94 35 45 " " `` cord-014901-d9szap94 35 46 edible edible JJ cord-014901-d9szap94 35 47 vaccines vaccine NNS cord-014901-d9szap94 35 48 . . . cord-014901-d9szap94 36 1 " " `` cord-014901-d9szap94 36 2 The the DT cord-014901-d9szap94 36 3 next next JJ cord-014901-d9szap94 36 4 important important JJ cord-014901-d9szap94 36 5 step step NN cord-014901-d9szap94 36 6 in in IN cord-014901-d9szap94 36 7 the the DT cord-014901-d9szap94 36 8 development development NN cord-014901-d9szap94 36 9 of of IN cord-014901-d9szap94 36 10 the the DT cord-014901-d9szap94 36 11 " " `` cord-014901-d9szap94 36 12 edible edible JJ cord-014901-d9szap94 36 13 vaccine vaccine NN cord-014901-d9szap94 36 14 " " '' cord-014901-d9szap94 36 15 concept concept NN cord-014901-d9szap94 36 16 on on IN cord-014901-d9szap94 36 17 the the DT cord-014901-d9szap94 36 18 basis basis NN cord-014901-d9szap94 36 19 of of IN cord-014901-d9szap94 36 20 genetically genetically RB cord-014901-d9szap94 36 21 modified modify VBN cord-014901-d9szap94 36 22 plants plant NNS cord-014901-d9szap94 36 23 was be VBD cord-014901-d9szap94 36 24 the the DT cord-014901-d9szap94 36 25 creation creation NN cord-014901-d9szap94 36 26 of of IN cord-014901-d9szap94 36 27 transgenic transgenic JJ cord-014901-d9szap94 36 28 plants plant NNS cord-014901-d9szap94 36 29 producing produce VBG cord-014901-d9szap94 36 30 the the DT cord-014901-d9szap94 36 31 heat heat NN cord-014901-d9szap94 36 32 labile labile JJ cord-014901-d9szap94 36 33 enterotoxin enterotoxin NN cord-014901-d9szap94 36 34 of of IN cord-014901-d9szap94 36 35 Escherichia Escherichia NNP cord-014901-d9szap94 36 36 coli coli NNS cord-014901-d9szap94 36 37 [ [ -LRB- cord-014901-d9szap94 36 38 6 6 CD cord-014901-d9szap94 36 39 , , , cord-014901-d9szap94 36 40 7 7 CD cord-014901-d9szap94 36 41 ] ] -RRB- cord-014901-d9szap94 36 42 and and CC cord-014901-d9szap94 36 43 B B NNP cord-014901-d9szap94 36 44 subunit subunit NN cord-014901-d9szap94 36 45 of of IN cord-014901-d9szap94 36 46 the the DT cord-014901-d9szap94 36 47 cholera cholera NN cord-014901-d9szap94 36 48 toxin toxin NN cord-014901-d9szap94 36 49 [ [ -LRB- cord-014901-d9szap94 36 50 8 8 CD cord-014901-d9szap94 36 51 ] ] -RRB- cord-014901-d9szap94 36 52 . . . cord-014901-d9szap94 37 1 Heat heat NN cord-014901-d9szap94 37 2 labile labile JJ cord-014901-d9szap94 37 3 toxin toxin NN cord-014901-d9szap94 37 4 of of IN cord-014901-d9szap94 37 5 E. E. NNP cord-014901-d9szap94 37 6 coli coli NNS cord-014901-d9szap94 37 7 consists consist VBZ cord-014901-d9szap94 37 8 of of IN cord-014901-d9szap94 37 9 two two CD cord-014901-d9szap94 37 10 parts part NNS cord-014901-d9szap94 37 11 : : : cord-014901-d9szap94 37 12 LT LT NNP cord-014901-d9szap94 37 13 A A NNP cord-014901-d9szap94 37 14 ( ( -LRB- cord-014901-d9szap94 37 15 enzyme enzyme NN cord-014901-d9szap94 37 16 ) ) -RRB- cord-014901-d9szap94 37 17 and and CC cord-014901-d9szap94 37 18 LT LT NNP cord-014901-d9szap94 37 19 B B NNP cord-014901-d9szap94 37 20 ( ( -LRB- cord-014901-d9szap94 37 21 pentamer pentamer NNP cord-014901-d9szap94 37 22 of of IN cord-014901-d9szap94 37 23 receptor receptor NN cord-014901-d9szap94 37 24 binding bind VBG cord-014901-d9szap94 37 25 polypeptides polypeptide NNS cord-014901-d9szap94 37 26 ) ) -RRB- cord-014901-d9szap94 37 27 . . . cord-014901-d9szap94 38 1 LT LT NNP cord-014901-d9szap94 38 2 B B NNP cord-014901-d9szap94 38 3 binds bind VBZ cord-014901-d9szap94 38 4 to to IN cord-014901-d9szap94 38 5 the the DT cord-014901-d9szap94 38 6 receptors receptor NNS cord-014901-d9szap94 38 7 on on IN cord-014901-d9szap94 38 8 the the DT cord-014901-d9szap94 38 9 sur sur FW cord-014901-d9szap94 38 10 face face NN cord-014901-d9szap94 38 11 of of IN cord-014901-d9szap94 38 12 membranes membrane NNS cord-014901-d9szap94 38 13 of of IN cord-014901-d9szap94 38 14 epithelial epithelial JJ cord-014901-d9szap94 38 15 cells cell NNS cord-014901-d9szap94 38 16 of of IN cord-014901-d9szap94 38 17 the the DT cord-014901-d9szap94 38 18 mammal mammal JJ cord-014901-d9szap94 38 19 small small JJ cord-014901-d9szap94 38 20 intestine intestine NN cord-014901-d9szap94 38 21 and and CC cord-014901-d9szap94 38 22 transports transport VBZ cord-014901-d9szap94 38 23 LT LT NNP cord-014901-d9szap94 38 24 A A NNP cord-014901-d9szap94 38 25 in in IN cord-014901-d9szap94 38 26 the the DT cord-014901-d9szap94 38 27 intestinal intestinal JJ cord-014901-d9szap94 38 28 cells cell NNS cord-014901-d9szap94 38 29 , , , cord-014901-d9szap94 38 30 where where WRB cord-014901-d9szap94 38 31 it -PRON- PRP cord-014901-d9szap94 38 32 induces induce VBZ cord-014901-d9szap94 38 33 changes change NNS cord-014901-d9szap94 38 34 in in IN cord-014901-d9szap94 38 35 the the DT cord-014901-d9szap94 38 36 cell cell NN cord-014901-d9szap94 38 37 metabolism metabolism NN cord-014901-d9szap94 38 38 and and CC cord-014901-d9szap94 38 39 cell cell NN cord-014901-d9szap94 38 40 dehydration dehydration NN cord-014901-d9szap94 38 41 . . . cord-014901-d9szap94 39 1 When when WRB cord-014901-d9szap94 39 2 the the DT cord-014901-d9szap94 39 3 two two CD cord-014901-d9szap94 39 4 parts part NNS cord-014901-d9szap94 39 5 of of IN cord-014901-d9szap94 39 6 the the DT cord-014901-d9szap94 39 7 heat heat NN cord-014901-d9szap94 39 8 labile labile JJ cord-014901-d9szap94 39 9 enterotoxin enterotoxin NNP cord-014901-d9szap94 39 10 are be VBP cord-014901-d9szap94 39 11 separated separate VBN cord-014901-d9szap94 39 12 , , , cord-014901-d9szap94 39 13 the the DT cord-014901-d9szap94 39 14 appearance appearance NN cord-014901-d9szap94 39 15 of of IN cord-014901-d9szap94 39 16 the the DT cord-014901-d9szap94 39 17 LT LT NNP cord-014901-d9szap94 39 18 B B NNP cord-014901-d9szap94 39 19 protein protein NN cord-014901-d9szap94 39 20 complex complex NN cord-014901-d9szap94 39 21 on on IN cord-014901-d9szap94 39 22 the the DT cord-014901-d9szap94 39 23 surface surface NN cord-014901-d9szap94 39 24 of of IN cord-014901-d9szap94 39 25 epitheliocytes epitheliocyte NNS cord-014901-d9szap94 39 26 will will MD cord-014901-d9szap94 39 27 stimulate stimulate VB cord-014901-d9szap94 39 28 a a DT cord-014901-d9szap94 39 29 strong strong JJ cord-014901-d9szap94 39 30 immune immune JJ cord-014901-d9szap94 39 31 response response NN cord-014901-d9szap94 39 32 of of IN cord-014901-d9szap94 39 33 intestine intestine NN cord-014901-d9szap94 39 34 mucosa mucosa NN cord-014901-d9szap94 39 35 without without IN cord-014901-d9szap94 39 36 the the DT cord-014901-d9szap94 39 37 appearance appearance NN cord-014901-d9szap94 39 38 of of IN cord-014901-d9szap94 39 39 any any DT cord-014901-d9szap94 39 40 disease disease NN cord-014901-d9szap94 39 41 signs sign NNS cord-014901-d9szap94 39 42 . . . cord-014901-d9szap94 40 1 Just just RB cord-014901-d9szap94 40 2 this this DT cord-014901-d9szap94 40 3 feature feature NN cord-014901-d9szap94 40 4 was be VBD cord-014901-d9szap94 40 5 the the DT cord-014901-d9szap94 40 6 basis basis NN cord-014901-d9szap94 40 7 for for IN cord-014901-d9szap94 40 8 the the DT cord-014901-d9szap94 40 9 research research NN cord-014901-d9szap94 40 10 of of IN cord-014901-d9szap94 40 11 C. C. NNP cord-014901-d9szap94 40 12 Arntzen Arntzen NNP cord-014901-d9szap94 40 13 [ [ -LRB- cord-014901-d9szap94 40 14 6 6 CD cord-014901-d9szap94 40 15 ] ] -RRB- cord-014901-d9szap94 40 16 team team NN cord-014901-d9szap94 40 17 on on IN cord-014901-d9szap94 40 18 the the DT cord-014901-d9szap94 40 19 creation creation NN cord-014901-d9szap94 40 20 of of IN cord-014901-d9szap94 40 21 plant plant NN cord-014901-d9szap94 40 22 vaccine vaccine NN cord-014901-d9szap94 40 23 providing provide VBG cord-014901-d9szap94 40 24 for for IN cord-014901-d9szap94 40 25 the the DT cord-014901-d9szap94 40 26 resistance resistance NN cord-014901-d9szap94 40 27 to to IN cord-014901-d9szap94 40 28 enterotoxigenic enterotoxigenic NNP cord-014901-d9szap94 40 29 E. E. NNP cord-014901-d9szap94 40 30 coli coli VBZ cord-014901-d9szap94 40 31 toxins toxin NNS cord-014901-d9szap94 40 32 . . . cord-014901-d9szap94 41 1 The the DT cord-014901-d9szap94 41 2 authors author NNS cord-014901-d9szap94 41 3 established establish VBD cord-014901-d9szap94 41 4 that that IN cord-014901-d9szap94 41 5 LT LT NNP cord-014901-d9szap94 41 6 B B NNP cord-014901-d9szap94 41 7 synthesized synthesize VBN cord-014901-d9szap94 41 8 in in IN cord-014901-d9szap94 41 9 transgenic transgenic JJ cord-014901-d9szap94 41 10 tobacco tobacco NN cord-014901-d9szap94 41 11 and and CC cord-014901-d9szap94 41 12 potato potato NN cord-014901-d9szap94 41 13 plants plant NNS cord-014901-d9szap94 41 14 and and CC cord-014901-d9szap94 41 15 also also RB cord-014901-d9szap94 41 16 LT LT NNP cord-014901-d9szap94 41 17 B B NNP cord-014901-d9szap94 41 18 isolated isolate VBN cord-014901-d9szap94 41 19 from from IN cord-014901-d9szap94 41 20 E. E. NNP cord-014901-d9szap94 41 21 coli coli NNS cord-014901-d9szap94 41 22 delivered deliver VBN cord-014901-d9szap94 41 23 orally orally RB cord-014901-d9szap94 41 24 to to IN cord-014901-d9szap94 41 25 mice mouse NNS cord-014901-d9szap94 41 26 induced induce VBN cord-014901-d9szap94 41 27 similar similar JJ cord-014901-d9szap94 41 28 immune immune JJ cord-014901-d9szap94 41 29 responses response NNS cord-014901-d9szap94 41 30 . . . cord-014901-d9szap94 42 1 Later later RB cord-014901-d9szap94 42 2 , , , cord-014901-d9szap94 42 3 LT LT NNP cord-014901-d9szap94 42 4 B B NNP cord-014901-d9szap94 42 5 sequence sequence NN cord-014901-d9szap94 42 6 was be VBD cord-014901-d9szap94 42 7 optimized optimize VBN cord-014901-d9szap94 42 8 for for IN cord-014901-d9szap94 42 9 expression expression NN cord-014901-d9szap94 42 10 in in IN cord-014901-d9szap94 42 11 plant plant NN cord-014901-d9szap94 42 12 cells cell NNS cord-014901-d9szap94 42 13 and and CC cord-014901-d9szap94 42 14 transferred transfer VBN cord-014901-d9szap94 42 15 into into IN cord-014901-d9szap94 42 16 the the DT cord-014901-d9szap94 42 17 potato potato NN cord-014901-d9szap94 42 18 genome genome NN cord-014901-d9szap94 42 19 [ [ -LRB- cord-014901-d9szap94 42 20 7 7 CD cord-014901-d9szap94 42 21 ] ] -RRB- cord-014901-d9szap94 42 22 . . . cord-014901-d9szap94 43 1 In in IN cord-014901-d9szap94 43 2 potato potato NN cord-014901-d9szap94 43 3 tubers tuber NNS cord-014901-d9szap94 43 4 , , , cord-014901-d9szap94 43 5 the the DT cord-014901-d9szap94 43 6 protein protein NN cord-014901-d9szap94 43 7 assembled assemble VBD cord-014901-d9szap94 43 8 correctly correctly RB cord-014901-d9szap94 43 9 in in IN cord-014901-d9szap94 43 10 oligomers oligomer NNS cord-014901-d9szap94 43 11 and and CC cord-014901-d9szap94 43 12 was be VBD cord-014901-d9szap94 43 13 accumulated accumulate VBN cord-014901-d9szap94 43 14 in in IN cord-014901-d9szap94 43 15 amounts amount NNS cord-014901-d9szap94 43 16 suffi suffi NNP cord-014901-d9szap94 43 17 cient cient NN cord-014901-d9szap94 43 18 for for IN cord-014901-d9szap94 43 19 the the DT cord-014901-d9szap94 43 20 induction induction NN cord-014901-d9szap94 43 21 of of IN cord-014901-d9szap94 43 22 the the DT cord-014901-d9szap94 43 23 immune immune JJ cord-014901-d9szap94 43 24 response response NN cord-014901-d9szap94 43 25 at at IN cord-014901-d9szap94 43 26 oral oral JJ cord-014901-d9szap94 43 27 delivery delivery NN cord-014901-d9szap94 43 28 to to IN cord-014901-d9szap94 43 29 the the DT cord-014901-d9szap94 43 30 organism organism NN cord-014901-d9szap94 43 31 . . . cord-014901-d9szap94 44 1 On on IN cord-014901-d9szap94 44 2 the the DT cord-014901-d9szap94 44 3 basis basis NN cord-014901-d9szap94 44 4 of of IN cord-014901-d9szap94 44 5 clinical clinical JJ cord-014901-d9szap94 44 6 trials trial NNS cord-014901-d9szap94 44 7 of of IN cord-014901-d9szap94 44 8 the the DT cord-014901-d9szap94 44 9 " " `` cord-014901-d9szap94 44 10 candidate candidate NN cord-014901-d9szap94 44 11 " " '' cord-014901-d9szap94 44 12 plant plant NN cord-014901-d9szap94 44 13 LT LT NNP cord-014901-d9szap94 44 14 B B NNP cord-014901-d9szap94 44 15 vaccine vaccine NN cord-014901-d9szap94 44 16 , , , cord-014901-d9szap94 44 17 it -PRON- PRP cord-014901-d9szap94 44 18 was be VBD cord-014901-d9szap94 44 19 estab estab NNP cord-014901-d9szap94 44 20 lished lishe VBN cord-014901-d9szap94 44 21 that that IN cord-014901-d9szap94 44 22 the the DT cord-014901-d9szap94 44 23 consumption consumption NN cord-014901-d9szap94 44 24 of of IN cord-014901-d9szap94 44 25 raw raw JJ cord-014901-d9szap94 44 26 potato potato NN cord-014901-d9szap94 44 27 tubers tuber NNS cord-014901-d9szap94 44 28 con con XX cord-014901-d9szap94 44 29 taining taining NN cord-014901-d9szap94 45 1 0.3 0.3 CD cord-014901-d9szap94 45 2 - - SYM cord-014901-d9szap94 45 3 10 10 CD cord-014901-d9szap94 45 4 mg mg NNP cord-014901-d9szap94 45 5 LT LT NNP cord-014901-d9szap94 45 6 B B NNP cord-014901-d9szap94 45 7 by by IN cord-014901-d9szap94 45 8 volunteers volunteer NNS cord-014901-d9szap94 45 9 resulted result VBD cord-014901-d9szap94 45 10 in in IN cord-014901-d9szap94 45 11 the the DT cord-014901-d9szap94 45 12 formation formation NN cord-014901-d9szap94 45 13 of of IN cord-014901-d9szap94 45 14 serum serum NN cord-014901-d9szap94 45 15 and and CC cord-014901-d9szap94 45 16 mucosal mucosal JJ cord-014901-d9szap94 45 17 immune immune JJ cord-014901-d9szap94 45 18 responses response NNS cord-014901-d9szap94 45 19 with with IN cord-014901-d9szap94 45 20 high high JJ cord-014901-d9szap94 45 21 titers titer NNS cord-014901-d9szap94 45 22 of of IN cord-014901-d9szap94 45 23 antibodies antibody NNS cord-014901-d9szap94 45 24 [ [ -LRB- cord-014901-d9szap94 45 25 9 9 CD cord-014901-d9szap94 45 26 ] ] -RRB- cord-014901-d9szap94 45 27 . . . cord-014901-d9szap94 46 1 The the DT cord-014901-d9szap94 46 2 initial initial JJ cord-014901-d9szap94 46 3 concept concept NN cord-014901-d9szap94 46 4 of of IN cord-014901-d9szap94 46 5 " " `` cord-014901-d9szap94 46 6 edible edible JJ cord-014901-d9szap94 46 7 vaccine vaccine NN cord-014901-d9szap94 46 8 " " '' cord-014901-d9szap94 46 9 was be VBD cord-014901-d9szap94 46 10 heavily heavily RB cord-014901-d9szap94 46 11 criticized criticize VBN cord-014901-d9szap94 46 12 by by IN cord-014901-d9szap94 46 13 researchers researcher NNS cord-014901-d9szap94 46 14 who who WP cord-014901-d9szap94 46 15 believed believe VBD cord-014901-d9szap94 46 16 that that IN cord-014901-d9szap94 46 17 in in IN cord-014901-d9szap94 46 18 the the DT cord-014901-d9szap94 46 19 aggressive aggressive JJ cord-014901-d9szap94 46 20 medium medium NN cord-014901-d9szap94 46 21 of of IN cord-014901-d9szap94 46 22 the the DT cord-014901-d9szap94 46 23 gastrointestinal gastrointestinal JJ cord-014901-d9szap94 46 24 tract tract NN cord-014901-d9szap94 46 25 the the DT cord-014901-d9szap94 46 26 recombinant recombinant JJ cord-014901-d9szap94 46 27 protein protein NN cord-014901-d9szap94 46 28 should should MD cord-014901-d9szap94 46 29 be be VB cord-014901-d9szap94 46 30 destroyed destroy VBN cord-014901-d9szap94 46 31 . . . cord-014901-d9szap94 47 1 However however RB cord-014901-d9szap94 47 2 , , , cord-014901-d9szap94 47 3 later later RB cord-014901-d9szap94 47 4 it -PRON- PRP cord-014901-d9szap94 47 5 was be VBD cord-014901-d9szap94 47 6 experimentally experimentally RB cord-014901-d9szap94 47 7 established establish VBN cord-014901-d9szap94 47 8 that that IN cord-014901-d9szap94 47 9 the the DT cord-014901-d9szap94 47 10 recom recom NNP cord-014901-d9szap94 47 11 binant binant NNP cord-014901-d9szap94 47 12 B B NNP cord-014901-d9szap94 47 13 subunit subunit NN cord-014901-d9szap94 47 14 of of IN cord-014901-d9szap94 47 15 the the DT cord-014901-d9szap94 47 16 cholera cholera NN cord-014901-d9szap94 47 17 toxin toxin NN cord-014901-d9szap94 47 18 fused fuse VBN cord-014901-d9szap94 47 19 with with IN cord-014901-d9szap94 47 20 green green JJ cord-014901-d9szap94 47 21 fluorescent fluorescent NN cord-014901-d9szap94 47 22 protein protein NN cord-014901-d9szap94 47 23 ( ( -LRB- cord-014901-d9szap94 47 24 GFP GFP NNP cord-014901-d9szap94 47 25 ) ) -RRB- cord-014901-d9szap94 47 26 protected protect VBN cord-014901-d9szap94 47 27 by by IN cord-014901-d9szap94 47 28 the the DT cord-014901-d9szap94 47 29 plant plant NN cord-014901-d9szap94 47 30 cellu cellu NNP cord-014901-d9szap94 47 31 lose lose VB cord-014901-d9szap94 47 32 cell cell NN cord-014901-d9szap94 47 33 wall wall NN cord-014901-d9szap94 47 34 at at IN cord-014901-d9szap94 47 35 oral oral JJ cord-014901-d9szap94 47 36 delivery delivery NN cord-014901-d9szap94 47 37 is be VBZ cord-014901-d9szap94 47 38 capable capable JJ cord-014901-d9szap94 47 39 of of IN cord-014901-d9szap94 47 40 passing pass VBG cord-014901-d9szap94 47 41 through through IN cord-014901-d9szap94 47 42 the the DT cord-014901-d9szap94 47 43 gastrointestinal gastrointestinal JJ cord-014901-d9szap94 47 44 tract tract NN cord-014901-d9szap94 47 45 and and CC cord-014901-d9szap94 47 46 reaching reach VBG cord-014901-d9szap94 47 47 the the DT cord-014901-d9szap94 47 48 antigen antigen NN cord-014901-d9szap94 47 49 containing contain VBG cord-014901-d9szap94 47 50 cells cell NNS cord-014901-d9szap94 47 51 of of IN cord-014901-d9szap94 47 52 the the DT cord-014901-d9szap94 47 53 mouse mouse NN cord-014901-d9szap94 47 54 intestine intestine NN cord-014901-d9szap94 47 55 [ [ -LRB- cord-014901-d9szap94 47 56 10 10 CD cord-014901-d9szap94 47 57 ] ] -RRB- cord-014901-d9szap94 47 58 . . . cord-014901-d9szap94 48 1 The the DT cord-014901-d9szap94 48 2 results result NNS cord-014901-d9szap94 48 3 obtained obtain VBD cord-014901-d9szap94 48 4 confirmed confirm VBD cord-014901-d9szap94 48 5 the the DT cord-014901-d9szap94 48 6 possibility possibility NN cord-014901-d9szap94 48 7 of of IN cord-014901-d9szap94 48 8 using use VBG cord-014901-d9szap94 48 9 plants plant NNS cord-014901-d9szap94 48 10 synthesizing synthesize VBG cord-014901-d9szap94 48 11 protein protein NN cord-014901-d9szap94 48 12 antigens antigen NNS cord-014901-d9szap94 48 13 of of IN cord-014901-d9szap94 48 14 various various JJ cord-014901-d9szap94 48 15 infec infec IN cord-014901-d9szap94 48 16 tious tious JJ cord-014901-d9szap94 48 17 agents agent NNS cord-014901-d9szap94 48 18 for for IN cord-014901-d9szap94 48 19 oral oral JJ cord-014901-d9szap94 48 20 delivery delivery NN cord-014901-d9szap94 48 21 of of IN cord-014901-d9szap94 48 22 antigens antigen NNS cord-014901-d9szap94 48 23 to to IN cord-014901-d9szap94 48 24 the the DT cord-014901-d9szap94 48 25 mucosa mucosa NN cord-014901-d9szap94 48 26 of of IN cord-014901-d9szap94 48 27 the the DT cord-014901-d9szap94 48 28 gastrointestinal gastrointestinal JJ cord-014901-d9szap94 48 29 tract tract NN cord-014901-d9szap94 48 30 and and CC cord-014901-d9szap94 48 31 experimentally experimentally RB cord-014901-d9szap94 48 32 con con NNP cord-014901-d9szap94 48 33 firmed firm VBD cord-014901-d9szap94 48 34 a a DT cord-014901-d9szap94 48 35 consistency consistency NN cord-014901-d9szap94 48 36 of of IN cord-014901-d9szap94 48 37 the the DT cord-014901-d9szap94 48 38 " " `` cord-014901-d9szap94 48 39 edible edible JJ cord-014901-d9szap94 48 40 vaccine vaccine NN cord-014901-d9szap94 48 41 " " '' cord-014901-d9szap94 48 42 concept concept NN cord-014901-d9szap94 48 43 . . . cord-014901-d9szap94 49 1 It -PRON- PRP cord-014901-d9szap94 49 2 became become VBD cord-014901-d9szap94 49 3 evident evident JJ cord-014901-d9szap94 49 4 that that IN cord-014901-d9szap94 49 5 genetically genetically RB cord-014901-d9szap94 49 6 modified modify VBN cord-014901-d9szap94 49 7 plants plant NNS cord-014901-d9szap94 49 8 can can MD cord-014901-d9szap94 49 9 be be VB cord-014901-d9szap94 49 10 used use VBN cord-014901-d9szap94 49 11 for for IN cord-014901-d9szap94 49 12 the the DT cord-014901-d9szap94 49 13 creation creation NN cord-014901-d9szap94 49 14 of of IN cord-014901-d9szap94 49 15 plant plant NN cord-014901-d9szap94 49 16 vaccines vaccine NNS cord-014901-d9szap94 49 17 as as IN cord-014901-d9szap94 49 18 a a DT cord-014901-d9szap94 49 19 raw raw JJ cord-014901-d9szap94 49 20 mate mate NN cord-014901-d9szap94 49 21 rial rial NN cord-014901-d9szap94 49 22 , , , cord-014901-d9szap94 49 23 and and CC cord-014901-d9szap94 49 24 separate separate JJ cord-014901-d9szap94 49 25 plant plant NN cord-014901-d9szap94 49 26 parts part NNS cord-014901-d9szap94 49 27 ( ( -LRB- cord-014901-d9szap94 49 28 fruits fruit NNS cord-014901-d9szap94 49 29 , , , cord-014901-d9szap94 49 30 roots root NNS cord-014901-d9szap94 49 31 , , , cord-014901-d9szap94 49 32 berries berry NNS cord-014901-d9szap94 49 33 , , , cord-014901-d9szap94 49 34 leaves leave NNS cord-014901-d9szap94 49 35 , , , cord-014901-d9szap94 49 36 etc etc FW cord-014901-d9szap94 49 37 . . . cord-014901-d9szap94 49 38 ) ) -RRB- cord-014901-d9szap94 49 39 can can MD cord-014901-d9szap94 49 40 be be VB cord-014901-d9szap94 49 41 used use VBN cord-014901-d9szap94 49 42 directly directly RB cord-014901-d9szap94 49 43 in in IN cord-014901-d9szap94 49 44 food food NN cord-014901-d9szap94 49 45 without without IN cord-014901-d9szap94 49 46 pre pre JJ cord-014901-d9szap94 49 47 liminary liminary JJ cord-014901-d9szap94 49 48 heat heat NN cord-014901-d9szap94 49 49 treatment treatment NN cord-014901-d9szap94 49 50 . . . cord-014901-d9szap94 50 1 RESPONSE response NN cord-014901-d9szap94 50 2 FORMATION FORMATION NNP cord-014901-d9szap94 51 1 In in IN cord-014901-d9szap94 51 2 the the DT cord-014901-d9szap94 51 3 process process NN cord-014901-d9szap94 51 4 of of IN cord-014901-d9szap94 51 5 evolution evolution NN cord-014901-d9szap94 51 6 , , , cord-014901-d9szap94 51 7 mammals mammal NNS cord-014901-d9szap94 51 8 developed develop VBD cord-014901-d9szap94 51 9 secondary secondary JJ cord-014901-d9szap94 51 10 lymphoid lymphoid JJ cord-014901-d9szap94 51 11 mucosal mucosal JJ cord-014901-d9szap94 51 12 tissue tissue NN cord-014901-d9szap94 51 13 capable capable JJ cord-014901-d9szap94 51 14 of of IN cord-014901-d9szap94 51 15 antigen antigen NN cord-014901-d9szap94 51 16 absorption absorption NN cord-014901-d9szap94 51 17 , , , cord-014901-d9szap94 51 18 processing process VBG cord-014901-d9szap94 51 19 them -PRON- PRP cord-014901-d9szap94 51 20 , , , cord-014901-d9szap94 51 21 and and CC cord-014901-d9szap94 51 22 using use VBG cord-014901-d9szap94 51 23 for for IN cord-014901-d9szap94 51 24 the the DT cord-014901-d9szap94 51 25 induc induc NNP cord-014901-d9szap94 51 26 tion tion NN cord-014901-d9szap94 51 27 of of IN cord-014901-d9szap94 51 28 the the DT cord-014901-d9szap94 51 29 mucosal mucosal JJ cord-014901-d9szap94 51 30 response response NN cord-014901-d9szap94 51 31 . . . cord-014901-d9szap94 52 1 It -PRON- PRP cord-014901-d9szap94 52 2 was be VBD cord-014901-d9szap94 52 3 established establish VBN cord-014901-d9szap94 52 4 that that IN cord-014901-d9szap94 52 5 in in IN cord-014901-d9szap94 52 6 this this DT cord-014901-d9szap94 52 7 case case NN cord-014901-d9szap94 52 8 both both CC cord-014901-d9szap94 52 9 cellular cellular JJ cord-014901-d9szap94 52 10 and and CC cord-014901-d9szap94 52 11 humoral humoral JJ cord-014901-d9szap94 52 12 immunity immunity NN cord-014901-d9szap94 52 13 were be VBD cord-014901-d9szap94 52 14 formed form VBN cord-014901-d9szap94 52 15 . . . cord-014901-d9szap94 53 1 It -PRON- PRP cord-014901-d9szap94 53 2 is be VBZ cord-014901-d9szap94 53 3 of of IN cord-014901-d9szap94 53 4 importance importance NN cord-014901-d9szap94 53 5 that that IN cord-014901-d9szap94 53 6 adaptive adaptive JJ cord-014901-d9szap94 53 7 mucosal mucosal JJ cord-014901-d9szap94 53 8 immunity immunity NN cord-014901-d9szap94 53 9 can can MD cord-014901-d9szap94 53 10 distinguish distinguish VB cord-014901-d9szap94 53 11 between between IN cord-014901-d9szap94 53 12 usual usual JJ cord-014901-d9szap94 53 13 food food NN cord-014901-d9szap94 53 14 and and CC cord-014901-d9szap94 53 15 symbiotic symbiotic JJ cord-014901-d9szap94 53 16 antigens antigen NNS cord-014901-d9szap94 53 17 and and CC cord-014901-d9szap94 53 18 infectious infectious JJ cord-014901-d9szap94 53 19 agents agent NNS cord-014901-d9szap94 53 20 [ [ -LRB- cord-014901-d9szap94 53 21 11 11 CD cord-014901-d9szap94 53 22 ] ] -RRB- cord-014901-d9szap94 53 23 . . . cord-014901-d9szap94 54 1 The the DT cord-014901-d9szap94 54 2 scheme scheme NN cord-014901-d9szap94 54 3 of of IN cord-014901-d9szap94 54 4 the the DT cord-014901-d9szap94 54 5 mechanism mechanism NN cord-014901-d9szap94 54 6 of of IN cord-014901-d9szap94 54 7 mucosal mucosal JJ cord-014901-d9szap94 54 8 immune immune JJ cord-014901-d9szap94 54 9 response response NN cord-014901-d9szap94 54 10 formation formation NN cord-014901-d9szap94 54 11 is be VBZ cord-014901-d9szap94 54 12 presented present VBN cord-014901-d9szap94 54 13 in in IN cord-014901-d9szap94 54 14 Fig Fig NNP cord-014901-d9szap94 54 15 . . . cord-014901-d9szap94 55 1 1 1 LS cord-014901-d9szap94 55 2 . . . cord-014901-d9szap94 56 1 In in IN cord-014901-d9szap94 56 2 the the DT cord-014901-d9szap94 56 3 digestive digestive JJ cord-014901-d9szap94 56 4 tract tract NN cord-014901-d9szap94 56 5 , , , cord-014901-d9szap94 56 6 which which WDT cord-014901-d9szap94 56 7 is be VBZ cord-014901-d9szap94 56 8 one one CD cord-014901-d9szap94 56 9 of of IN cord-014901-d9szap94 56 10 the the DT cord-014901-d9szap94 56 11 pathways pathway NNS cord-014901-d9szap94 56 12 for for IN cord-014901-d9szap94 56 13 the the DT cord-014901-d9szap94 56 14 penetration penetration NN cord-014901-d9szap94 56 15 of of IN cord-014901-d9szap94 56 16 a a DT cord-014901-d9szap94 56 17 variety variety NN cord-014901-d9szap94 56 18 of of IN cord-014901-d9szap94 56 19 pathogens pathogen NNS cord-014901-d9szap94 56 20 into into IN cord-014901-d9szap94 56 21 organism organism NN cord-014901-d9szap94 56 22 , , , cord-014901-d9szap94 56 23 the the DT cord-014901-d9szap94 56 24 main main JJ cord-014901-d9szap94 56 25 associated associated JJ cord-014901-d9szap94 56 26 lymphoid lymphoid JJ cord-014901-d9szap94 56 27 tissue tissue NN cord-014901-d9szap94 56 28 is be VBZ cord-014901-d9szap94 56 29 the the DT cord-014901-d9szap94 56 30 Peyer Peyer NNP cord-014901-d9szap94 56 31 's 's POS cord-014901-d9szap94 56 32 patches patch NNS cord-014901-d9szap94 56 33 . . . cord-014901-d9szap94 57 1 Peyer Peyer NNP cord-014901-d9szap94 57 2 's 's POS cord-014901-d9szap94 57 3 patches patch NNS cord-014901-d9szap94 57 4 , , , cord-014901-d9szap94 57 5 inductive inductive JJ cord-014901-d9szap94 57 6 sites site NNS cord-014901-d9szap94 57 7 of of IN cord-014901-d9szap94 57 8 the the DT cord-014901-d9szap94 57 9 intestine intestine NN cord-014901-d9szap94 57 10 , , , cord-014901-d9szap94 57 11 which which WDT cord-014901-d9szap94 57 12 contain contain VBP cord-014901-d9szap94 57 13 the the DT cord-014901-d9szap94 57 14 dome dome NN cord-014901-d9szap94 57 15 , , , cord-014901-d9szap94 57 16 underlying underlie VBG cord-014901-d9szap94 57 17 the the DT cord-014901-d9szap94 57 18 fol fol NNP cord-014901-d9szap94 57 19 licle licle NNP cord-014901-d9szap94 57 20 ( ( -LRB- cord-014901-d9szap94 57 21 B B NNP cord-014901-d9szap94 57 22 zones zone NNS cord-014901-d9szap94 57 23 with with IN cord-014901-d9szap94 57 24 germinal germinal JJ cord-014901-d9szap94 57 25 center center NN cord-014901-d9szap94 57 26 ) ) -RRB- cord-014901-d9szap94 57 27 and and CC cord-014901-d9szap94 57 28 interfollicu interfollicu NNP cord-014901-d9szap94 57 29 lar lar NNP cord-014901-d9szap94 57 30 region region NN cord-014901-d9szap94 57 31 containing contain VBG cord-014901-d9szap94 57 32 T t NN cord-014901-d9szap94 57 33 cells cell NNS cord-014901-d9szap94 57 34 . . . cord-014901-d9szap94 58 1 The the DT cord-014901-d9szap94 58 2 surface surface NN cord-014901-d9szap94 58 3 of of IN cord-014901-d9szap94 58 4 the the DT cord-014901-d9szap94 58 5 dome dome NN cord-014901-d9szap94 58 6 is be VBZ cord-014901-d9szap94 58 7 covered cover VBN cord-014901-d9szap94 58 8 by by IN cord-014901-d9szap94 58 9 a a DT cord-014901-d9szap94 58 10 specialized specialized JJ cord-014901-d9szap94 58 11 follicle follicle NN cord-014901-d9szap94 58 12 associated associate VBD cord-014901-d9szap94 58 13 epithe epithe NNP cord-014901-d9szap94 58 14 lium lium NNP cord-014901-d9szap94 58 15 containing contain VBG cord-014901-d9szap94 58 16 the the DT cord-014901-d9szap94 58 17 folded fold VBN cord-014901-d9szap94 58 18 cells cell NNS cord-014901-d9szap94 58 19 ( ( -LRB- cord-014901-d9szap94 58 20 M M NNP cord-014901-d9szap94 58 21 cells cell NNS cord-014901-d9szap94 58 22 ) ) -RRB- cord-014901-d9szap94 58 23 , , , cord-014901-d9szap94 58 24 which which WDT cord-014901-d9szap94 58 25 are be VBP cord-014901-d9szap94 58 26 able able JJ cord-014901-d9szap94 58 27 to to TO cord-014901-d9szap94 58 28 absorb absorb VB cord-014901-d9szap94 58 29 and and CC cord-014901-d9szap94 58 30 transport transport NN cord-014901-d9szap94 58 31 antigens antigen NNS cord-014901-d9szap94 58 32 from from IN cord-014901-d9szap94 58 33 the the DT cord-014901-d9szap94 58 34 intesti intesti NNP cord-014901-d9szap94 58 35 nal nal NNP cord-014901-d9szap94 58 36 lumen lumen NNP cord-014901-d9szap94 58 37 . . . cord-014901-d9szap94 59 1 After after IN cord-014901-d9szap94 59 2 successful successful JJ cord-014901-d9szap94 59 3 capture capture NN cord-014901-d9szap94 59 4 , , , cord-014901-d9szap94 59 5 the the DT cord-014901-d9szap94 59 6 antigen antigen NN cord-014901-d9szap94 59 7 is be VBZ cord-014901-d9szap94 59 8 par par NN cord-014901-d9szap94 59 9 tially tially RB cord-014901-d9szap94 59 10 cleaved cleave VBN cord-014901-d9szap94 59 11 and and CC cord-014901-d9szap94 59 12 enters enter VBZ cord-014901-d9szap94 59 13 into into IN cord-014901-d9szap94 59 14 dendritic dendritic JJ cord-014901-d9szap94 59 15 cells cell NNS cord-014901-d9szap94 59 16 ( ( -LRB- cord-014901-d9szap94 59 17 see see VB cord-014901-d9szap94 59 18 Fig Fig NNP cord-014901-d9szap94 59 19 . . . cord-014901-d9szap94 60 1 1 1 LS cord-014901-d9szap94 60 2 , , , cord-014901-d9szap94 60 3 step step NN cord-014901-d9szap94 60 4 1 1 CD cord-014901-d9szap94 60 5 ) ) -RRB- cord-014901-d9szap94 60 6 . . . cord-014901-d9szap94 61 1 Dendritic dendritic JJ cord-014901-d9szap94 61 2 cells cell NNS cord-014901-d9szap94 61 3 are be VBP cord-014901-d9szap94 61 4 especially especially RB cord-014901-d9szap94 61 5 important important JJ cord-014901-d9szap94 61 6 in in IN cord-014901-d9szap94 61 7 the the DT cord-014901-d9szap94 61 8 initiation initiation NN cord-014901-d9szap94 61 9 of of IN cord-014901-d9szap94 61 10 adaptive adaptive JJ cord-014901-d9szap94 61 11 immune immune JJ cord-014901-d9szap94 61 12 responses response NNS cord-014901-d9szap94 61 13 , , , cord-014901-d9szap94 61 14 since since IN cord-014901-d9szap94 61 15 they -PRON- PRP cord-014901-d9szap94 61 16 migrate migrate VBP cord-014901-d9szap94 61 17 to to IN cord-014901-d9szap94 61 18 the the DT cord-014901-d9szap94 61 19 lymph lymph NN cord-014901-d9szap94 61 20 nodes node NNS cord-014901-d9szap94 61 21 ( ( -LRB- cord-014901-d9szap94 61 22 see see VB cord-014901-d9szap94 61 23 Fig Fig NNP cord-014901-d9szap94 61 24 . . . cord-014901-d9szap94 62 1 1 1 LS cord-014901-d9szap94 62 2 , , , cord-014901-d9szap94 62 3 step step NN cord-014901-d9szap94 62 4 2 2 CD cord-014901-d9szap94 62 5 ) ) -RRB- cord-014901-d9szap94 62 6 , , , cord-014901-d9szap94 62 7 and and CC cord-014901-d9szap94 62 8 the the DT cord-014901-d9szap94 62 9 mediators mediator NNS cord-014901-d9szap94 62 10 act act VBP cord-014901-d9szap94 62 11 in in IN cord-014901-d9szap94 62 12 the the DT cord-014901-d9szap94 62 13 development development NN cord-014901-d9szap94 62 14 of of IN cord-014901-d9szap94 62 15 various various JJ cord-014901-d9szap94 62 16 subpopu subpopu NN cord-014901-d9szap94 62 17 lations lation NNS cord-014901-d9szap94 62 18 of of IN cord-014901-d9szap94 62 19 T T NNP cord-014901-d9szap94 62 20 helper helper NN cord-014901-d9szap94 62 21 cells cell NNS cord-014901-d9szap94 62 22 from from IN cord-014901-d9szap94 62 23 naive naive JJ cord-014901-d9szap94 62 24 T t NN cord-014901-d9szap94 62 25 lymphocytes lymphocyte NNS cord-014901-d9szap94 62 26 and and CC cord-014901-d9szap94 62 27 also also RB cord-014901-d9szap94 62 28 can can MD cord-014901-d9szap94 62 29 interact interact VB cord-014901-d9szap94 62 30 with with IN cord-014901-d9szap94 62 31 B b NN cord-014901-d9szap94 62 32 lymphocytes lymphocyte NNS cord-014901-d9szap94 62 33 ( ( -LRB- cord-014901-d9szap94 62 34 see see VB cord-014901-d9szap94 62 35 Fig Fig NNP cord-014901-d9szap94 62 36 . . . cord-014901-d9szap94 63 1 1 1 LS cord-014901-d9szap94 63 2 , , , cord-014901-d9szap94 63 3 step step NN cord-014901-d9szap94 63 4 5 5 CD cord-014901-d9szap94 63 5 ) ) -RRB- cord-014901-d9szap94 63 6 . . . cord-014901-d9szap94 64 1 The the DT cord-014901-d9szap94 64 2 activated activate VBN cord-014901-d9szap94 64 3 B B NNP cord-014901-d9szap94 64 4 and and CC cord-014901-d9szap94 64 5 T t NN cord-014901-d9szap94 64 6 cells cell NNS cord-014901-d9szap94 64 7 leave leave VBP cord-014901-d9szap94 64 8 the the DT cord-014901-d9szap94 64 9 Peyer Peyer NNP cord-014901-d9szap94 64 10 's 's POS cord-014901-d9szap94 64 11 patches patch NNS cord-014901-d9szap94 64 12 and and CC cord-014901-d9szap94 64 13 penetrate penetrate VB cord-014901-d9szap94 64 14 into into IN cord-014901-d9szap94 64 15 the the DT cord-014901-d9szap94 64 16 circulatory circulatory JJ cord-014901-d9szap94 64 17 system system NN cord-014901-d9szap94 64 18 . . . cord-014901-d9szap94 65 1 Mature mature JJ cord-014901-d9szap94 65 2 T T NNP cord-014901-d9szap94 65 3 helper helper NN cord-014901-d9szap94 65 4 cells cell NNS cord-014901-d9szap94 65 5 then then RB cord-014901-d9szap94 65 6 return return VBP cord-014901-d9szap94 65 7 to to IN cord-014901-d9szap94 65 8 the the DT cord-014901-d9szap94 65 9 mucosa mucosa JJ cord-014901-d9szap94 65 10 sur sur FW cord-014901-d9szap94 65 11 Pathogens Pathogens NNP cord-014901-d9szap94 65 12 ( ( -LRB- cord-014901-d9szap94 65 13 antigen antigen NN cord-014901-d9szap94 65 14 ) ) -RRB- cord-014901-d9szap94 65 15 Epithelium epithelium NN cord-014901-d9szap94 65 16 T T NNP cord-014901-d9szap94 65 17 helper helper NN cord-014901-d9szap94 65 18 cells cell NNS cord-014901-d9szap94 65 19 face face VBP cord-014901-d9szap94 65 20 to to TO cord-014901-d9szap94 65 21 function function VB cord-014901-d9szap94 65 22 as as IN cord-014901-d9szap94 65 23 effectors effector NNS cord-014901-d9szap94 65 24 ( ( -LRB- cord-014901-d9szap94 65 25 see see VB cord-014901-d9szap94 65 26 Fig Fig NNP cord-014901-d9szap94 65 27 . . . cord-014901-d9szap94 66 1 1 1 LS cord-014901-d9szap94 66 2 , , , cord-014901-d9szap94 66 3 step step NN cord-014901-d9szap94 66 4 3 3 CD cord-014901-d9szap94 66 5 ) ) -RRB- cord-014901-d9szap94 66 6 . . . cord-014901-d9szap94 67 1 T T NNP cord-014901-d9szap94 67 2 helper helper NN cord-014901-d9szap94 67 3 17 17 CD cord-014901-d9szap94 67 4 expressing express VBG cord-014901-d9szap94 67 5 interleukin interleukin NN cord-014901-d9szap94 67 6 17 17 CD cord-014901-d9szap94 68 1 ( ( -LRB- cord-014901-d9szap94 68 2 IL IL NNP cord-014901-d9szap94 68 3 17 17 CD cord-014901-d9szap94 68 4 ) ) -RRB- cord-014901-d9szap94 68 5 increases increase VBZ cord-014901-d9szap94 68 6 the the DT cord-014901-d9szap94 68 7 expression expression NN cord-014901-d9szap94 68 8 of of IN cord-014901-d9szap94 68 9 the the DT cord-014901-d9szap94 68 10 receptors receptor NNS cord-014901-d9szap94 68 11 of of IN cord-014901-d9szap94 68 12 polymeric polymeric JJ cord-014901-d9szap94 68 13 immunoglobulin immunoglobulin NN cord-014901-d9szap94 68 14 ( ( -LRB- cord-014901-d9szap94 68 15 pIg pIg NNP cord-014901-d9szap94 68 16 ) ) -RRB- cord-014901-d9szap94 69 1 and and CC cord-014901-d9szap94 69 2 secretion secretion NN cord-014901-d9szap94 69 3 of of IN cord-014901-d9szap94 69 4 antigen antigen NNP cord-014901-d9szap94 69 5 spe spe NNP cord-014901-d9szap94 69 6 cific cific NNP cord-014901-d9szap94 69 7 IgA IgA NNP cord-014901-d9szap94 69 8 ( ( -LRB- cord-014901-d9szap94 69 9 Fig Fig NNP cord-014901-d9szap94 69 10 . . NNP cord-014901-d9szap94 69 11 1 1 CD cord-014901-d9szap94 69 12 , , , cord-014901-d9szap94 69 13 step step NN cord-014901-d9szap94 69 14 4 4 CD cord-014901-d9szap94 69 15 ) ) -RRB- cord-014901-d9szap94 69 16 . . . cord-014901-d9szap94 70 1 Subsequent subsequent JJ cord-014901-d9szap94 70 2 generation generation NN cord-014901-d9szap94 70 3 of of IN cord-014901-d9szap94 70 4 mature mature JJ cord-014901-d9szap94 70 5 plasma plasma NN cord-014901-d9szap94 70 6 cells cell NNS cord-014901-d9szap94 70 7 producing produce VBG cord-014901-d9szap94 70 8 IgA IgA NNP cord-014901-d9szap94 70 9 leads lead VBZ cord-014901-d9szap94 70 10 to to IN cord-014901-d9szap94 70 11 the the DT cord-014901-d9szap94 70 12 induction induction NN cord-014901-d9szap94 70 13 of of IN cord-014901-d9szap94 70 14 antigen antigen NN cord-014901-d9szap94 70 15 specific specific JJ cord-014901-d9szap94 70 16 protection protection NN cord-014901-d9szap94 70 17 of of IN cord-014901-d9szap94 70 18 local local JJ cord-014901-d9szap94 70 19 and and CC cord-014901-d9szap94 70 20 distal distal JJ cord-014901-d9szap94 70 21 mucosal mucosal JJ cord-014901-d9szap94 70 22 surfaces surface NNS cord-014901-d9szap94 70 23 . . . cord-014901-d9szap94 71 1 Since since IN cord-014901-d9szap94 71 2 the the DT cord-014901-d9szap94 71 3 mucosal mucosal JJ cord-014901-d9szap94 71 4 immune immune JJ cord-014901-d9szap94 71 5 response response NN cord-014901-d9szap94 71 6 has have VBZ cord-014901-d9szap94 71 7 a a DT cord-014901-d9szap94 71 8 general general JJ cord-014901-d9szap94 71 9 ized ized JJ cord-014901-d9szap94 71 10 nature nature NN cord-014901-d9szap94 71 11 , , . cord-014901-d9szap94 72 1 oral oral JJ cord-014901-d9szap94 72 2 ( ( -LRB- cord-014901-d9szap94 72 3 i.e. i.e. FW cord-014901-d9szap94 72 4 , , , cord-014901-d9szap94 72 5 mucosal mucosal JJ cord-014901-d9szap94 72 6 ) ) -RRB- cord-014901-d9szap94 72 7 vaccination vaccination NN cord-014901-d9szap94 72 8 is be VBZ cord-014901-d9szap94 72 9 not not RB cord-014901-d9szap94 72 10 only only RB cord-014901-d9szap94 72 11 the the DT cord-014901-d9szap94 72 12 immune immune JJ cord-014901-d9szap94 72 13 response response NN cord-014901-d9szap94 72 14 of of IN cord-014901-d9szap94 72 15 the the DT cord-014901-d9szap94 72 16 mucous mucous JJ cord-014901-d9szap94 72 17 membranes membrane NNS cord-014901-d9szap94 72 18 , , , cord-014901-d9szap94 72 19 but but CC cord-014901-d9szap94 72 20 also also RB cord-014901-d9szap94 72 21 the the DT cord-014901-d9szap94 72 22 overall overall JJ cord-014901-d9szap94 72 23 immune immune JJ cord-014901-d9szap94 72 24 response response NN cord-014901-d9szap94 72 25 of of IN cord-014901-d9szap94 72 26 the the DT cord-014901-d9szap94 72 27 organism organism NN cord-014901-d9szap94 72 28 [ [ -LRB- cord-014901-d9szap94 72 29 11 11 CD cord-014901-d9szap94 72 30 ] ] -RRB- cord-014901-d9szap94 72 31 [ [ -LRB- cord-014901-d9szap94 72 32 12 12 CD cord-014901-d9szap94 72 33 ] ] -RRB- cord-014901-d9szap94 72 34 [ [ -LRB- cord-014901-d9szap94 72 35 13 13 CD cord-014901-d9szap94 72 36 ] ] -RRB- cord-014901-d9szap94 72 37 [ [ -LRB- cord-014901-d9szap94 72 38 14 14 CD cord-014901-d9szap94 72 39 ] ] -RRB- cord-014901-d9szap94 72 40 . . . cord-014901-d9szap94 73 1 It -PRON- PRP cord-014901-d9szap94 73 2 is be VBZ cord-014901-d9szap94 73 3 proved prove VBN cord-014901-d9szap94 73 4 that that IN cord-014901-d9szap94 73 5 vaccination vaccination NN cord-014901-d9szap94 73 6 via via IN cord-014901-d9szap94 73 7 the the DT cord-014901-d9szap94 73 8 surface surface NN cord-014901-d9szap94 73 9 of of IN cord-014901-d9szap94 73 10 mucosa mucosa JJ cord-014901-d9szap94 73 11 membrane membrane NN cord-014901-d9szap94 73 12 can can MD cord-014901-d9szap94 73 13 specifically specifically RB cord-014901-d9szap94 73 14 activate activate VB cord-014901-d9szap94 73 15 the the DT cord-014901-d9szap94 73 16 immune immune JJ cord-014901-d9szap94 73 17 response response NN cord-014901-d9szap94 73 18 to to IN cord-014901-d9szap94 73 19 infection infection NN cord-014901-d9szap94 73 20 without without IN cord-014901-d9szap94 73 21 the the DT cord-014901-d9szap94 73 22 develop develop NN cord-014901-d9szap94 73 23 ment ment NN cord-014901-d9szap94 73 24 of of IN cord-014901-d9szap94 73 25 such such JJ cord-014901-d9szap94 73 26 processes process NNS cord-014901-d9szap94 73 27 related relate VBN cord-014901-d9szap94 73 28 to to IN cord-014901-d9szap94 73 29 the the DT cord-014901-d9szap94 73 30 disease disease NN cord-014901-d9szap94 73 31 as as IN cord-014901-d9szap94 73 32 inflammation inflammation NN cord-014901-d9szap94 73 33 or or CC cord-014901-d9szap94 73 34 toxicity toxicity NN cord-014901-d9szap94 73 35 [ [ -LRB- cord-014901-d9szap94 73 36 14 14 CD cord-014901-d9szap94 73 37 , , , cord-014901-d9szap94 73 38 15 15 CD cord-014901-d9szap94 73 39 ] ] -RRB- cord-014901-d9szap94 73 40 . . . cord-014901-d9szap94 74 1 OF of IN cord-014901-d9szap94 74 2 PLANT plant NN cord-014901-d9szap94 74 3 VACCINES vaccine NNS cord-014901-d9szap94 75 1 In in IN cord-014901-d9szap94 75 2 comparison comparison NN cord-014901-d9szap94 75 3 with with IN cord-014901-d9szap94 75 4 traditional traditional JJ cord-014901-d9szap94 75 5 expression expression NN cord-014901-d9szap94 75 6 systems system NNS cord-014901-d9szap94 75 7 , , , cord-014901-d9szap94 75 8 plant plant NN cord-014901-d9szap94 75 9 systems system NNS cord-014901-d9szap94 75 10 are be VBP cord-014901-d9szap94 75 11 attractive attractive JJ cord-014901-d9szap94 75 12 to to IN cord-014901-d9szap94 75 13 researchers researcher NNS cord-014901-d9szap94 75 14 in in IN cord-014901-d9szap94 75 15 many many JJ cord-014901-d9szap94 75 16 ways way NNS cord-014901-d9szap94 75 17 , , , cord-014901-d9szap94 75 18 primarily primarily RB cord-014901-d9szap94 75 19 due due IN cord-014901-d9szap94 75 20 to to IN cord-014901-d9szap94 75 21 the the DT cord-014901-d9szap94 75 22 absence absence NN cord-014901-d9szap94 75 23 of of IN cord-014901-d9szap94 75 24 the the DT cord-014901-d9szap94 75 25 risk risk NN cord-014901-d9szap94 75 26 of of IN cord-014901-d9szap94 75 27 plant plant NN cord-014901-d9szap94 75 28 cell cell NN cord-014901-d9szap94 75 29 infection infection NN cord-014901-d9szap94 75 30 with with IN cord-014901-d9szap94 75 31 animal animal NN cord-014901-d9szap94 75 32 pathogens pathogen NNS cord-014901-d9szap94 75 33 , , , cord-014901-d9szap94 75 34 viruses virus NNS cord-014901-d9szap94 75 35 , , , cord-014901-d9szap94 75 36 prions prion NNS cord-014901-d9szap94 75 37 , , , cord-014901-d9szap94 75 38 etc etc FW cord-014901-d9szap94 75 39 . . . cord-014901-d9szap94 76 1 Plants plant NNS cord-014901-d9szap94 76 2 are be VBP cord-014901-d9szap94 76 3 capable capable JJ cord-014901-d9szap94 76 4 of of IN cord-014901-d9szap94 76 5 the the DT cord-014901-d9szap94 76 6 synthesis synthesis NN cord-014901-d9szap94 76 7 of of IN cord-014901-d9szap94 76 8 most most JJS cord-014901-d9szap94 76 9 recom recom NNP cord-014901-d9szap94 76 10 binant binant JJ cord-014901-d9szap94 76 11 antigens antigen NNS cord-014901-d9szap94 76 12 with with IN cord-014901-d9szap94 76 13 the the DT cord-014901-d9szap94 76 14 same same JJ cord-014901-d9szap94 76 15 posttranslational posttranslational JJ cord-014901-d9szap94 76 16 modi modi NNP cord-014901-d9szap94 76 17 fications fication NNS cord-014901-d9szap94 76 18 as as IN cord-014901-d9szap94 76 19 in in IN cord-014901-d9szap94 76 20 animal animal NN cord-014901-d9szap94 76 21 cells cell NNS cord-014901-d9szap94 76 22 [ [ -LRB- cord-014901-d9szap94 76 23 16 16 CD cord-014901-d9szap94 76 24 ] ] -RRB- cord-014901-d9szap94 76 25 . . . cord-014901-d9szap94 77 1 Plant plant NN cord-014901-d9szap94 77 2 vaccines vaccine NNS cord-014901-d9szap94 77 3 can can MD cord-014901-d9szap94 77 4 play play VB cord-014901-d9szap94 77 5 especially especially RB cord-014901-d9szap94 77 6 important important JJ cord-014901-d9szap94 77 7 role role NN cord-014901-d9szap94 77 8 in in IN cord-014901-d9szap94 77 9 the the DT cord-014901-d9szap94 77 10 protection protection NN cord-014901-d9szap94 77 11 of of IN cord-014901-d9szap94 77 12 ani ani NNP cord-014901-d9szap94 77 13 mals mals NNP cord-014901-d9szap94 77 14 against against IN cord-014901-d9szap94 77 15 diarrheal diarrheal JJ cord-014901-d9szap94 77 16 diseases disease NNS cord-014901-d9szap94 77 17 and and CC cord-014901-d9szap94 77 18 diseases disease NNS cord-014901-d9szap94 77 19 , , , cord-014901-d9szap94 77 20 which which WDT cord-014901-d9szap94 77 21 infectious infectious JJ cord-014901-d9szap94 77 22 agents agent NNS cord-014901-d9szap94 77 23 penetrate penetrate VBP cord-014901-d9szap94 77 24 into into IN cord-014901-d9szap94 77 25 the the DT cord-014901-d9szap94 77 26 organism organism NN cord-014901-d9szap94 77 27 through through IN cord-014901-d9szap94 77 28 the the DT cord-014901-d9szap94 77 29 mucosal mucosal JJ cord-014901-d9szap94 77 30 tissues tissue NNS cord-014901-d9szap94 77 31 . . . cord-014901-d9szap94 78 1 Modern modern JJ cord-014901-d9szap94 78 2 techniques technique NNS cord-014901-d9szap94 78 3 of of IN cord-014901-d9szap94 78 4 genetic genetic JJ cord-014901-d9szap94 78 5 engineering engineering NN cord-014901-d9szap94 78 6 allow allow VBP cord-014901-d9szap94 78 7 to to TO cord-014901-d9szap94 78 8 selectively selectively RB cord-014901-d9szap94 78 9 direct direct VB cord-014901-d9szap94 78 10 the the DT cord-014901-d9szap94 78 11 recombinant recombinant JJ cord-014901-d9szap94 78 12 proteins protein NNS cord-014901-d9szap94 78 13 expressed express VBN cord-014901-d9szap94 78 14 in in IN cord-014901-d9szap94 78 15 plant plant NN cord-014901-d9szap94 78 16 cells cell NNS cord-014901-d9szap94 78 17 to to IN cord-014901-d9szap94 78 18 various various JJ cord-014901-d9szap94 78 19 plant plant NN cord-014901-d9szap94 78 20 organs organ NNS cord-014901-d9szap94 78 21 ( ( -LRB- cord-014901-d9szap94 78 22 seeds seed NNS cord-014901-d9szap94 78 23 , , , cord-014901-d9szap94 78 24 tubers tuber NNS cord-014901-d9szap94 78 25 , , , cord-014901-d9szap94 78 26 fruits fruit NNS cord-014901-d9szap94 78 27 , , , cord-014901-d9szap94 78 28 etc etc FW cord-014901-d9szap94 78 29 . . . cord-014901-d9szap94 78 30 ) ) -RRB- cord-014901-d9szap94 79 1 [ [ -LRB- cord-014901-d9szap94 79 2 17 17 CD cord-014901-d9szap94 79 3 ] ] -RRB- cord-014901-d9szap94 79 4 . . . cord-014901-d9szap94 80 1 This this DT cord-014901-d9szap94 80 2 possibility possibility NN cord-014901-d9szap94 80 3 greatly greatly RB cord-014901-d9szap94 80 4 simplifies simplify VBZ cord-014901-d9szap94 80 5 the the DT cord-014901-d9szap94 80 6 large large JJ cord-014901-d9szap94 80 7 scale scale NN cord-014901-d9szap94 80 8 production production NN cord-014901-d9szap94 80 9 of of IN cord-014901-d9szap94 80 10 plant plant NN cord-014901-d9szap94 80 11 vaccines vaccine NNS cord-014901-d9szap94 80 12 and and CC cord-014901-d9szap94 80 13 reduces reduce VBZ cord-014901-d9szap94 80 14 their -PRON- PRP$ cord-014901-d9szap94 80 15 cost cost NN cord-014901-d9szap94 80 16 . . . cord-014901-d9szap94 81 1 According accord VBG cord-014901-d9szap94 81 2 to to IN cord-014901-d9szap94 81 3 the the DT cord-014901-d9szap94 81 4 experts expert NNS cord-014901-d9szap94 81 5 , , , cord-014901-d9szap94 81 6 the the DT cord-014901-d9szap94 81 7 final final JJ cord-014901-d9szap94 81 8 price price NN cord-014901-d9szap94 81 9 of of IN cord-014901-d9szap94 81 10 the the DT cord-014901-d9szap94 81 11 product product NN cord-014901-d9szap94 81 12 ( ( -LRB- cord-014901-d9szap94 81 13 recombinant recombinant JJ cord-014901-d9szap94 81 14 protein protein NN cord-014901-d9szap94 81 15 ) ) -RRB- cord-014901-d9szap94 81 16 produced produce VBD cord-014901-d9szap94 81 17 in in IN cord-014901-d9szap94 81 18 a a DT cord-014901-d9szap94 81 19 plant plant NN cord-014901-d9szap94 81 20 expression expression NN cord-014901-d9szap94 81 21 system system NN cord-014901-d9szap94 81 22 will will MD cord-014901-d9szap94 81 23 be be VB cord-014901-d9szap94 81 24 much much RB cord-014901-d9szap94 81 25 less less JJR cord-014901-d9szap94 81 26 than than IN cord-014901-d9szap94 81 27 the the DT cord-014901-d9szap94 81 28 price price NN cord-014901-d9szap94 81 29 of of IN cord-014901-d9szap94 81 30 a a DT cord-014901-d9szap94 81 31 similar similar JJ cord-014901-d9szap94 81 32 protein protein NN cord-014901-d9szap94 81 33 produced produce VBN cord-014901-d9szap94 81 34 , , , cord-014901-d9szap94 81 35 for for IN cord-014901-d9szap94 81 36 example example NN cord-014901-d9szap94 81 37 , , , cord-014901-d9szap94 81 38 in in IN cord-014901-d9szap94 81 39 mammalian mammalian JJ cord-014901-d9szap94 81 40 cell cell NN cord-014901-d9szap94 81 41 culture culture NN cord-014901-d9szap94 81 42 [ [ -LRB- cord-014901-d9szap94 81 43 18 18 CD cord-014901-d9szap94 81 44 ] ] -RRB- cord-014901-d9szap94 81 45 . . . cord-014901-d9szap94 82 1 Since since IN cord-014901-d9szap94 82 2 recombinant recombinant JJ cord-014901-d9szap94 82 3 proteins protein NNS cord-014901-d9szap94 82 4 can can MD cord-014901-d9szap94 82 5 be be VB cord-014901-d9szap94 82 6 accumulated accumulate VBN cord-014901-d9szap94 82 7 in in IN cord-014901-d9szap94 82 8 the the DT cord-014901-d9szap94 82 9 storage storage NN cord-014901-d9szap94 82 10 organs organ NNS cord-014901-d9szap94 82 11 or or CC cord-014901-d9szap94 82 12 seeds seed NNS cord-014901-d9szap94 82 13 , , , cord-014901-d9szap94 82 14 they -PRON- PRP cord-014901-d9szap94 82 15 are be VBP cord-014901-d9szap94 82 16 able able JJ cord-014901-d9szap94 82 17 to to TO cord-014901-d9szap94 82 18 be be VB cord-014901-d9szap94 82 19 main main JJ cord-014901-d9szap94 82 20 tained taine VBN cord-014901-d9szap94 82 21 without without IN cord-014901-d9szap94 82 22 any any DT cord-014901-d9szap94 82 23 changes change NNS cord-014901-d9szap94 82 24 and and CC cord-014901-d9szap94 82 25 the the DT cord-014901-d9szap94 82 26 loss loss NN cord-014901-d9szap94 82 27 of of IN cord-014901-d9szap94 82 28 biological biological JJ cord-014901-d9szap94 82 29 activity activity NN cord-014901-d9szap94 82 30 for for IN cord-014901-d9szap94 82 31 a a DT cord-014901-d9szap94 82 32 long long JJ cord-014901-d9szap94 82 33 time time NN cord-014901-d9szap94 82 34 ( ( -LRB- cord-014901-d9szap94 82 35 months month NNS cord-014901-d9szap94 82 36 and and CC cord-014901-d9szap94 82 37 years year NNS cord-014901-d9szap94 82 38 ) ) -RRB- cord-014901-d9szap94 82 39 . . . cord-014901-d9szap94 83 1 It -PRON- PRP cord-014901-d9szap94 83 2 was be VBD cord-014901-d9szap94 83 3 established establish VBN cord-014901-d9szap94 83 4 that that IN cord-014901-d9szap94 83 5 the the DT cord-014901-d9szap94 83 6 recombinant recombinant JJ cord-014901-d9szap94 83 7 protein protein NN cord-014901-d9szap94 83 8 of of IN cord-014901-d9szap94 83 9 cholera cholera NN cord-014901-d9szap94 83 10 toxin toxin NN cord-014901-d9szap94 83 11 B B NNP cord-014901-d9szap94 83 12 subunit subunit NN cord-014901-d9szap94 83 13 remained remain VBD cord-014901-d9szap94 83 14 stable stable JJ cord-014901-d9szap94 83 15 in in IN cord-014901-d9szap94 83 16 transgenic transgenic JJ cord-014901-d9szap94 83 17 rice rice NN cord-014901-d9szap94 83 18 grains grain NNS cord-014901-d9szap94 83 19 for for IN cord-014901-d9szap94 83 20 at at RB cord-014901-d9szap94 83 21 least least RBS cord-014901-d9szap94 83 22 18 18 CD cord-014901-d9szap94 83 23 months month NNS cord-014901-d9szap94 83 24 , , , cord-014901-d9szap94 83 25 when when WRB cord-014901-d9szap94 83 26 grains grain NNS cord-014901-d9szap94 83 27 were be VBD cord-014901-d9szap94 83 28 stored store VBN cord-014901-d9szap94 83 29 under under IN cord-014901-d9szap94 83 30 room room NN cord-014901-d9szap94 83 31 temperature temperature NN cord-014901-d9szap94 83 32 conditions condition NNS cord-014901-d9szap94 83 33 [ [ -LRB- cord-014901-d9szap94 83 34 19 19 CD cord-014901-d9szap94 83 35 ] ] -RRB- cord-014901-d9szap94 83 36 . . . cord-014901-d9szap94 84 1 Recombi Recombi NNP cord-014901-d9szap94 84 2 nant nant NNP cord-014901-d9szap94 84 3 protein protein NN cord-014901-d9szap94 84 4 antigens antigen NNS cord-014901-d9szap94 84 5 remained remain VBD cord-014901-d9szap94 84 6 stable stable JJ cord-014901-d9szap94 84 7 in in IN cord-014901-d9szap94 84 8 rice rice NN cord-014901-d9szap94 84 9 grains grain NNS cord-014901-d9szap94 84 10 during during IN cord-014901-d9szap94 84 11 three three CD cord-014901-d9szap94 84 12 years year NNS cord-014901-d9szap94 84 13 and and CC cord-014901-d9szap94 84 14 provided provide VBN cord-014901-d9szap94 84 15 for for IN cord-014901-d9szap94 84 16 the the DT cord-014901-d9szap94 84 17 formation formation NN cord-014901-d9szap94 84 18 of of IN cord-014901-d9szap94 84 19 the the DT cord-014901-d9szap94 84 20 protective protective JJ cord-014901-d9szap94 84 21 immunity immunity NN cord-014901-d9szap94 84 22 in in IN cord-014901-d9szap94 84 23 mice mouse NNS cord-014901-d9szap94 84 24 against against IN cord-014901-d9szap94 84 25 cholera cholera NN cord-014901-d9szap94 84 26 agent agent NN cord-014901-d9szap94 84 27 or or CC cord-014901-d9szap94 84 28 against against IN cord-014901-d9szap94 84 29 enterotoxigenic enterotoxigenic NNP cord-014901-d9szap94 84 30 E. E. NNP cord-014901-d9szap94 84 31 coli coli NNS cord-014901-d9szap94 84 32 [ [ -LRB- cord-014901-d9szap94 84 33 20 20 CD cord-014901-d9szap94 84 34 ] ] -RRB- cord-014901-d9szap94 84 35 ; ; : cord-014901-d9szap94 84 36 in in IN cord-014901-d9szap94 84 37 soybean soybean NN cord-014901-d9szap94 84 38 seeds seed NNS cord-014901-d9szap94 84 39 and and CC cord-014901-d9szap94 84 40 soy soy NN cord-014901-d9szap94 84 41 milk milk NN cord-014901-d9szap94 84 42 , , , cord-014901-d9szap94 84 43 the the DT cord-014901-d9szap94 84 44 preservation preservation NN cord-014901-d9szap94 84 45 of of IN cord-014901-d9szap94 84 46 antigen antigen NN cord-014901-d9szap94 84 47 stabil stabil FW cord-014901-d9szap94 84 48 ity ity NNP cord-014901-d9szap94 84 49 was be VBD cord-014901-d9szap94 84 50 observed observe VBN cord-014901-d9szap94 84 51 for for IN cord-014901-d9szap94 84 52 four four CD cord-014901-d9szap94 84 53 years year NNS cord-014901-d9szap94 84 54 [ [ -LRB- cord-014901-d9szap94 84 55 21 21 CD cord-014901-d9szap94 84 56 ] ] -RRB- cord-014901-d9szap94 84 57 .Thus .Thus . cord-014901-d9szap94 85 1 , , , cord-014901-d9szap94 85 2 grains grain NNS cord-014901-d9szap94 85 3 of of IN cord-014901-d9szap94 85 4 transgenic transgenic JJ cord-014901-d9szap94 85 5 plants plant NNS cord-014901-d9szap94 85 6 can can MD cord-014901-d9szap94 85 7 be be VB cord-014901-d9szap94 85 8 transported transport VBN cord-014901-d9szap94 85 9 to to IN cord-014901-d9szap94 85 10 the the DT cord-014901-d9szap94 85 11 site site NN cord-014901-d9szap94 85 12 of of IN cord-014901-d9szap94 85 13 final final JJ cord-014901-d9szap94 85 14 destination destination NN cord-014901-d9szap94 85 15 without without IN cord-014901-d9szap94 85 16 additional additional JJ cord-014901-d9szap94 85 17 freezing freezing NN cord-014901-d9szap94 85 18 and and CC cord-014901-d9szap94 85 19 treatment treatment NN cord-014901-d9szap94 85 20 , , , cord-014901-d9szap94 85 21 and and CC cord-014901-d9szap94 85 22 this this DT cord-014901-d9szap94 85 23 ensures ensure VBZ cord-014901-d9szap94 85 24 the the DT cord-014901-d9szap94 85 25 retention retention NN cord-014901-d9szap94 85 26 of of IN cord-014901-d9szap94 85 27 activity activity NN cord-014901-d9szap94 85 28 of of IN cord-014901-d9szap94 85 29 the the DT cord-014901-d9szap94 85 30 recom recom NNP cord-014901-d9szap94 85 31 binant binant NNP cord-014901-d9szap94 85 32 protein protein NN cord-014901-d9szap94 85 33 activity activity NN cord-014901-d9szap94 85 34 , , , cord-014901-d9szap94 85 35 its -PRON- PRP$ cord-014901-d9szap94 85 36 stability stability NN cord-014901-d9szap94 85 37 , , , cord-014901-d9szap94 85 38 and and CC cord-014901-d9szap94 85 39 the the DT cord-014901-d9szap94 85 40 constancy constancy NN cord-014901-d9szap94 85 41 of of IN cord-014901-d9szap94 85 42 dosage dosage NN cord-014901-d9szap94 85 43 . . . cord-014901-d9szap94 86 1 A a DT cord-014901-d9szap94 86 2 somewhat somewhat RB cord-014901-d9szap94 86 3 different different JJ cord-014901-d9szap94 86 4 picture picture NN cord-014901-d9szap94 86 5 is be VBZ cord-014901-d9szap94 86 6 observed observe VBN cord-014901-d9szap94 86 7 when when WRB cord-014901-d9szap94 86 8 lyo lyo NNP cord-014901-d9szap94 86 9 philization philization NNP cord-014901-d9szap94 86 10 is be VBZ cord-014901-d9szap94 86 11 used use VBN cord-014901-d9szap94 86 12 as as IN cord-014901-d9szap94 86 13 a a DT cord-014901-d9szap94 86 14 method method NN cord-014901-d9szap94 86 15 of of IN cord-014901-d9szap94 86 16 the the DT cord-014901-d9szap94 86 17 conservation conservation NN cord-014901-d9szap94 86 18 of of IN cord-014901-d9szap94 86 19 protein protein NN cord-014901-d9szap94 86 20 antigens antigen NNS cord-014901-d9szap94 86 21 synthesized synthesize VBN cord-014901-d9szap94 86 22 by by IN cord-014901-d9szap94 86 23 plant plant NN cord-014901-d9szap94 86 24 cells cell NNS cord-014901-d9szap94 86 25 . . . cord-014901-d9szap94 87 1 It -PRON- PRP cord-014901-d9szap94 87 2 was be VBD cord-014901-d9szap94 87 3 established establish VBN cord-014901-d9szap94 87 4 that that IN cord-014901-d9szap94 87 5 the the DT cord-014901-d9szap94 87 6 recombinant recombinant JJ cord-014901-d9szap94 87 7 protein protein NN cord-014901-d9szap94 87 8 of of IN cord-014901-d9szap94 87 9 the the DT cord-014901-d9szap94 87 10 norovirus norovirus NN cord-014901-d9szap94 87 11 envelope envelope NN cord-014901-d9szap94 87 12 retained retain VBD cord-014901-d9szap94 87 13 its -PRON- PRP$ cord-014901-d9szap94 87 14 immunogenicity immunogenicity NN cord-014901-d9szap94 87 15 in in IN cord-014901-d9szap94 87 16 tis tis NNP cord-014901-d9szap94 87 17 sues sue NNS cord-014901-d9szap94 87 18 of of IN cord-014901-d9szap94 87 19 both both DT cord-014901-d9szap94 87 20 lyophilized lyophilize VBN cord-014901-d9szap94 87 21 and and CC cord-014901-d9szap94 87 22 air air NN cord-014901-d9szap94 87 23 dried dry VBN cord-014901-d9szap94 87 24 tomato tomato NN cord-014901-d9szap94 87 25 fruits fruit NNS cord-014901-d9szap94 87 26 [ [ -LRB- cord-014901-d9szap94 87 27 22 22 CD cord-014901-d9szap94 87 28 ] ] -RRB- cord-014901-d9szap94 87 29 , , , cord-014901-d9szap94 87 30 but but CC cord-014901-d9szap94 87 31 the the DT cord-014901-d9szap94 87 32 tested test VBN cord-014901-d9szap94 87 33 samples sample NNS cord-014901-d9szap94 87 34 differed differ VBD cord-014901-d9szap94 87 35 in in IN cord-014901-d9szap94 87 36 immunogenic immunogenic JJ cord-014901-d9szap94 87 37 ity ity NN cord-014901-d9szap94 87 38 . . . cord-014901-d9szap94 88 1 The the DT cord-014901-d9szap94 88 2 immunogenicity immunogenicity NN cord-014901-d9szap94 88 3 of of IN cord-014901-d9szap94 88 4 this this DT cord-014901-d9szap94 88 5 recombinant recombinant JJ cord-014901-d9szap94 88 6 protein protein NN cord-014901-d9szap94 88 7 in in IN cord-014901-d9szap94 88 8 air air NN cord-014901-d9szap94 88 9 dried dry VBN cord-014901-d9szap94 88 10 tomato tomato NN cord-014901-d9szap94 88 11 fruits fruit NNS cord-014901-d9szap94 88 12 was be VBD cord-014901-d9szap94 88 13 somewhat somewhat RB cord-014901-d9szap94 88 14 higher high JJR cord-014901-d9szap94 88 15 in in IN cord-014901-d9szap94 88 16 comparison comparison NN cord-014901-d9szap94 88 17 with with IN cord-014901-d9szap94 88 18 lyophilized lyophilized JJ cord-014901-d9szap94 88 19 fruits fruit NNS cord-014901-d9szap94 88 20 . . . cord-014901-d9szap94 89 1 Similar similar JJ cord-014901-d9szap94 89 2 results result NNS cord-014901-d9szap94 89 3 were be VBD cord-014901-d9szap94 89 4 obtained obtain VBN cord-014901-d9szap94 89 5 in in IN cord-014901-d9szap94 89 6 experiments experiment NNS cord-014901-d9szap94 89 7 on on IN cord-014901-d9szap94 89 8 the the DT cord-014901-d9szap94 89 9 lyophilization lyophilization NN cord-014901-d9szap94 89 10 of of IN cord-014901-d9szap94 89 11 potato potato NN cord-014901-d9szap94 89 12 tubers tuber NNS cord-014901-d9szap94 89 13 synthesizing synthesize VBG cord-014901-d9szap94 89 14 the the DT cord-014901-d9szap94 89 15 protein protein NN cord-014901-d9szap94 89 16 of of IN cord-014901-d9szap94 89 17 norovirus norovirus NN cord-014901-d9szap94 89 18 envelope envelope NN cord-014901-d9szap94 89 19 , , , cord-014901-d9szap94 89 20 e.g. e.g. RB cord-014901-d9szap94 89 21 , , , cord-014901-d9szap94 89 22 the the DT cord-014901-d9szap94 89 23 immunogenicity immunogenicity NN cord-014901-d9szap94 89 24 of of IN cord-014901-d9szap94 89 25 lyophilized lyophilized JJ cord-014901-d9szap94 89 26 tubers tuber NNS cord-014901-d9szap94 89 27 was be VBD cord-014901-d9szap94 89 28 lower low JJR cord-014901-d9szap94 89 29 than than IN cord-014901-d9szap94 89 30 that that DT cord-014901-d9szap94 89 31 of of IN cord-014901-d9szap94 89 32 fresh fresh JJ cord-014901-d9szap94 89 33 tubers tuber NNS cord-014901-d9szap94 89 34 [ [ -LRB- cord-014901-d9szap94 89 35 22 22 CD cord-014901-d9szap94 89 36 ] ] -RRB- cord-014901-d9szap94 89 37 . . . cord-014901-d9szap94 90 1 How how WRB cord-014901-d9szap94 90 2 ever ever RB cord-014901-d9szap94 90 3 , , , cord-014901-d9szap94 90 4 additional additional JJ cord-014901-d9szap94 90 5 studies study NNS cord-014901-d9szap94 90 6 are be VBP cord-014901-d9szap94 90 7 required require VBN cord-014901-d9szap94 90 8 for for IN cord-014901-d9szap94 90 9 the the DT cord-014901-d9szap94 90 10 final final JJ cord-014901-d9szap94 90 11 solu solu NNP cord-014901-d9szap94 90 12 tion tion NN cord-014901-d9szap94 90 13 of of IN cord-014901-d9szap94 90 14 this this DT cord-014901-d9szap94 90 15 question question NN cord-014901-d9szap94 90 16 . . . cord-014901-d9szap94 91 1 Despite despite IN cord-014901-d9szap94 91 2 these these DT cord-014901-d9szap94 91 3 advantages advantage NNS cord-014901-d9szap94 91 4 , , , cord-014901-d9szap94 91 5 plant plant NN cord-014901-d9szap94 91 6 vaccines vaccine NNS cord-014901-d9szap94 91 7 are be VBP cord-014901-d9szap94 91 8 not not RB cord-014901-d9szap94 91 9 without without IN cord-014901-d9szap94 91 10 disadvantages disadvantage NNS cord-014901-d9szap94 91 11 . . . cord-014901-d9szap94 92 1 One one CD cord-014901-d9szap94 92 2 of of IN cord-014901-d9szap94 92 3 them -PRON- PRP cord-014901-d9szap94 92 4 is be VBZ cord-014901-d9szap94 92 5 differences difference NNS cord-014901-d9szap94 92 6 in in IN cord-014901-d9szap94 92 7 protein protein NN cord-014901-d9szap94 92 8 posttranslational posttranslational JJ cord-014901-d9szap94 92 9 modifications modification NNS cord-014901-d9szap94 92 10 in in IN cord-014901-d9szap94 92 11 plants plant NNS cord-014901-d9szap94 92 12 and and CC cord-014901-d9szap94 92 13 animals animal NNS cord-014901-d9szap94 92 14 , , , cord-014901-d9szap94 92 15 e.g. e.g. RB cord-014901-d9szap94 92 16 , , , cord-014901-d9szap94 92 17 in in IN cord-014901-d9szap94 92 18 glycosylation glycosylation NN cord-014901-d9szap94 92 19 of of IN cord-014901-d9szap94 92 20 the the DT cord-014901-d9szap94 92 21 recombinant recombinant JJ cord-014901-d9szap94 92 22 pro pro FW cord-014901-d9szap94 92 23 tein tein NNP cord-014901-d9szap94 92 24 [ [ -LRB- cord-014901-d9szap94 92 25 23 23 CD cord-014901-d9szap94 92 26 ] ] -RRB- cord-014901-d9szap94 92 27 . . . cord-014901-d9szap94 93 1 It -PRON- PRP cord-014901-d9szap94 93 2 is be VBZ cord-014901-d9szap94 93 3 known know VBN cord-014901-d9szap94 93 4 that that IN cord-014901-d9szap94 93 5 more more JJR cord-014901-d9szap94 93 6 than than IN cord-014901-d9szap94 93 7 a a DT cord-014901-d9szap94 93 8 half half NN cord-014901-d9szap94 93 9 of of IN cord-014901-d9szap94 93 10 proteins protein NNS cord-014901-d9szap94 93 11 synthesized synthesize VBN cord-014901-d9szap94 93 12 by by IN cord-014901-d9szap94 93 13 eukaryotic eukaryotic JJ cord-014901-d9szap94 93 14 cells cell NNS cord-014901-d9szap94 93 15 are be VBP cord-014901-d9szap94 93 16 glycosylated glycosylate VBN cord-014901-d9szap94 93 17 and and CC cord-014901-d9szap94 93 18 more more JJR cord-014901-d9szap94 93 19 than than IN cord-014901-d9szap94 93 20 a a DT cord-014901-d9szap94 93 21 third third NN cord-014901-d9szap94 93 22 of of IN cord-014901-d9szap94 93 23 currently currently RB cord-014901-d9szap94 93 24 applied apply VBN cord-014901-d9szap94 93 25 biopharmaceu biopharmaceu NNP cord-014901-d9szap94 93 26 tics tic NNS cord-014901-d9szap94 93 27 are be VBP cord-014901-d9szap94 93 28 glycoproteins glycoprotein NNS cord-014901-d9szap94 93 29 [ [ -LRB- cord-014901-d9szap94 93 30 24 24 CD cord-014901-d9szap94 93 31 ] ] -RRB- cord-014901-d9szap94 93 32 . . . cord-014901-d9szap94 94 1 Although although IN cord-014901-d9szap94 94 2 the the DT cord-014901-d9szap94 94 3 activity activity NN cord-014901-d9szap94 94 4 of of IN cord-014901-d9szap94 94 5 most most JJS cord-014901-d9szap94 94 6 proteins protein NNS cord-014901-d9szap94 94 7 does do VBZ cord-014901-d9szap94 94 8 not not RB cord-014901-d9szap94 94 9 depend depend VB cord-014901-d9szap94 94 10 on on IN cord-014901-d9szap94 94 11 glycosylation glycosylation NN cord-014901-d9szap94 94 12 , , , cord-014901-d9szap94 94 13 in in IN cord-014901-d9szap94 94 14 some some DT cord-014901-d9szap94 94 15 cases case NNS cord-014901-d9szap94 94 16 it -PRON- PRP cord-014901-d9szap94 94 17 may may MD cord-014901-d9szap94 94 18 be be VB cord-014901-d9szap94 94 19 critical critical JJ cord-014901-d9szap94 94 20 . . . cord-014901-d9szap94 95 1 Specific specific JJ cord-014901-d9szap94 95 2 features feature NNS cord-014901-d9szap94 95 3 of of IN cord-014901-d9szap94 95 4 pro pro NNP cord-014901-d9szap94 95 5 tein tein NNP cord-014901-d9szap94 95 6 glycoforms glycoform NNS cord-014901-d9szap94 95 7 can can MD cord-014901-d9szap94 95 8 affect affect VB cord-014901-d9szap94 95 9 their -PRON- PRP$ cord-014901-d9szap94 95 10 folding folding NN cord-014901-d9szap94 95 11 , , , cord-014901-d9szap94 95 12 stability stability NN cord-014901-d9szap94 95 13 , , , cord-014901-d9szap94 95 14 trans trans NNP cord-014901-d9szap94 95 15 portation portation NNP cord-014901-d9szap94 95 16 , , , cord-014901-d9szap94 95 17 and and CC cord-014901-d9szap94 95 18 changes change NNS cord-014901-d9szap94 95 19 in in IN cord-014901-d9szap94 95 20 their -PRON- PRP$ cord-014901-d9szap94 95 21 functional functional JJ cord-014901-d9szap94 95 22 activity activity NN cord-014901-d9szap94 95 23 and and CC cord-014901-d9szap94 95 24 immunogenicity immunogenicity NN cord-014901-d9szap94 95 25 . . . cord-014901-d9szap94 96 1 Examples example NNS cord-014901-d9szap94 96 2 of of IN cord-014901-d9szap94 96 3 biopharmaceuticals biopharmaceutical NNS cord-014901-d9szap94 96 4 , , , cord-014901-d9szap94 96 5 which which WDT cord-014901-d9szap94 96 6 functional functional JJ cord-014901-d9szap94 96 7 activity activity NN cord-014901-d9szap94 96 8 depends depend VBZ cord-014901-d9szap94 96 9 on on IN cord-014901-d9szap94 96 10 the the DT cord-014901-d9szap94 96 11 specific specific JJ cord-014901-d9szap94 96 12 gly gly NNP cord-014901-d9szap94 96 13 coform coform NNP cord-014901-d9szap94 96 14 , , , cord-014901-d9szap94 96 15 are be VBP cord-014901-d9szap94 96 16 erythropoietin erythropoietin NN cord-014901-d9szap94 96 17 , , , cord-014901-d9szap94 96 18 antibodies antibody NNS cord-014901-d9szap94 96 19 , , , cord-014901-d9szap94 96 20 blood blood NN cord-014901-d9szap94 96 21 anti anti JJ cord-014901-d9szap94 96 22 gens gen NNS cord-014901-d9szap94 96 23 , , , cord-014901-d9szap94 96 24 some some DT cord-014901-d9szap94 96 25 interferons interferon NNS cord-014901-d9szap94 96 26 and and CC cord-014901-d9szap94 96 27 hormones hormone NNS cord-014901-d9szap94 96 28 [ [ -LRB- cord-014901-d9szap94 96 29 24 24 CD cord-014901-d9szap94 96 30 ] ] -RRB- cord-014901-d9szap94 96 31 . . . cord-014901-d9szap94 97 1 Scheme scheme NN cord-014901-d9szap94 97 2 of of IN cord-014901-d9szap94 97 3 the the DT cord-014901-d9szap94 97 4 N n CD cord-014901-d9szap94 97 5 glycan glycan NN cord-014901-d9szap94 97 6 complex complex JJ cord-014901-d9szap94 97 7 formation formation NN cord-014901-d9szap94 97 8 in in IN cord-014901-d9szap94 97 9 plants plant NNS cord-014901-d9szap94 97 10 and and CC cord-014901-d9szap94 97 11 animals animal NNS cord-014901-d9szap94 97 12 ( ( -LRB- cord-014901-d9szap94 97 13 humans human NNS cord-014901-d9szap94 97 14 , , , cord-014901-d9szap94 97 15 for for IN cord-014901-d9szap94 97 16 example example NN cord-014901-d9szap94 97 17 ) ) -RRB- cord-014901-d9szap94 97 18 is be VBZ cord-014901-d9szap94 97 19 shown show VBN cord-014901-d9szap94 97 20 in in IN cord-014901-d9szap94 97 21 Fig Fig NNP cord-014901-d9szap94 97 22 . . . cord-014901-d9szap94 98 1 2 2 LS cord-014901-d9szap94 98 2 . . . cord-014901-d9szap94 99 1 The the DT cord-014901-d9szap94 99 2 most most RBS cord-014901-d9szap94 99 3 significant significant JJ cord-014901-d9szap94 99 4 difference difference NN cord-014901-d9szap94 99 5 in in IN cord-014901-d9szap94 99 6 glycosylation glycosylation NN cord-014901-d9szap94 99 7 is be VBZ cord-014901-d9szap94 99 8 that that DT cord-014901-d9szap94 100 1 the the DT cord-014901-d9szap94 100 2 plant plant NN cord-014901-d9szap94 100 3 β β NN cord-014901-d9szap94 101 1 1,2 1,2 CD cord-014901-d9szap94 101 2 xylose xylose NNP cord-014901-d9szap94 101 3 is be VBZ cord-014901-d9szap94 101 4 attached attach VBN cord-014901-d9szap94 101 5 to to IN cord-014901-d9szap94 101 6 the the DT cord-014901-d9szap94 101 7 core core NN cord-014901-d9szap94 101 8 mannose mannose NN cord-014901-d9szap94 101 9 residue residue NN cord-014901-d9szap94 101 10 and and CC cord-014901-d9szap94 101 11 α α NN cord-014901-d9szap94 101 12 1,3 1,3 CD cord-014901-d9szap94 101 13 fucose fucose NNP cord-014901-d9szap94 101 14 -to -to ADD cord-014901-d9szap94 102 1 N N NNP cord-014901-d9szap94 102 2 acetylglu acetylglu NNP cord-014901-d9szap94 102 3 cosamine cosamine NN cord-014901-d9szap94 102 4 residue residue NN cord-014901-d9szap94 102 5 of of IN cord-014901-d9szap94 102 6 the the DT cord-014901-d9szap94 102 7 core core NN cord-014901-d9szap94 102 8 glycan glycan NN cord-014901-d9szap94 102 9 . . . cord-014901-d9szap94 103 1 In in IN cord-014901-d9szap94 103 2 human human JJ cord-014901-d9szap94 103 3 cells cell NNS cord-014901-d9szap94 103 4 xylose xylose NN cord-014901-d9szap94 103 5 is be VBZ cord-014901-d9szap94 103 6 not not RB cord-014901-d9szap94 103 7 used use VBN cord-014901-d9szap94 103 8 at at RB cord-014901-d9szap94 103 9 all all RB cord-014901-d9szap94 103 10 in in IN cord-014901-d9szap94 103 11 glycosylation glycosylation NN cord-014901-d9szap94 103 12 and and CC cord-014901-d9szap94 103 13 a a DT cord-014901-d9szap94 103 14 proximal proximal JJ cord-014901-d9szap94 103 15 fucose fucose JJ cord-014901-d9szap94 103 16 residue residue NN cord-014901-d9szap94 103 17 is be VBZ cord-014901-d9szap94 103 18 attached attach VBN cord-014901-d9szap94 103 19 to to IN cord-014901-d9szap94 103 20 glycans glycan NNS cord-014901-d9szap94 103 21 through through IN cord-014901-d9szap94 103 22 the the DT cord-014901-d9szap94 103 23 α α NN cord-014901-d9szap94 103 24 1,6 1,6 CD cord-014901-d9szap94 103 25 bond bond NN cord-014901-d9szap94 103 26 . . . cord-014901-d9szap94 104 1 It -PRON- PRP cord-014901-d9szap94 104 2 was be VBD cord-014901-d9szap94 104 3 established establish VBN cord-014901-d9szap94 104 4 that that IN cord-014901-d9szap94 104 5 sugar sugar NN cord-014901-d9szap94 104 6 residues residue NNS cord-014901-d9szap94 104 7 attached attach VBN cord-014901-d9szap94 104 8 at at IN cord-014901-d9szap94 104 9 posttranslational posttranslational JJ cord-014901-d9szap94 104 10 modifications modification NNS cord-014901-d9szap94 104 11 of of IN cord-014901-d9szap94 104 12 recom recom NNP cord-014901-d9szap94 104 13 binant binant JJ cord-014901-d9szap94 104 14 proteins protein NNS cord-014901-d9szap94 104 15 in in IN cord-014901-d9szap94 104 16 plant plant NN cord-014901-d9szap94 104 17 cells cell NNS cord-014901-d9szap94 104 18 themselves -PRON- PRP cord-014901-d9szap94 104 19 were be VBD cord-014901-d9szap94 104 20 capable capable JJ cord-014901-d9szap94 104 21 of of IN cord-014901-d9szap94 104 22 exhibiting exhibit VBG cord-014901-d9szap94 104 23 the the DT cord-014901-d9szap94 104 24 immunogenicity immunogenicity NN cord-014901-d9szap94 104 25 . . . cord-014901-d9szap94 105 1 Approximately approximately RB cord-014901-d9szap94 105 2 in in IN cord-014901-d9szap94 105 3 one one CD cord-014901-d9szap94 105 4 quarter quarter NN cord-014901-d9szap94 105 5 of of IN cord-014901-d9szap94 105 6 patients patient NNS cord-014901-d9szap94 105 7 with with IN cord-014901-d9szap94 105 8 allergy allergy NN cord-014901-d9szap94 105 9 symptoms symptom NNS cord-014901-d9szap94 105 10 , , , cord-014901-d9szap94 105 11 IgE IgE NNP cord-014901-d9szap94 105 12 antibodies antibody NNS cord-014901-d9szap94 105 13 specific specific JJ cord-014901-d9szap94 105 14 for for IN cord-014901-d9szap94 105 15 complex complex JJ cord-014901-d9szap94 105 16 glycans glycan NNS cord-014901-d9szap94 105 17 , , , cord-014901-d9szap94 105 18 which which WDT cord-014901-d9szap94 105 19 include include VBP cord-014901-d9szap94 105 20 xylose xylose NN cord-014901-d9szap94 105 21 or or CC cord-014901-d9szap94 105 22 fucose fucose NNP cord-014901-d9szap94 105 23 , , , cord-014901-d9szap94 105 24 were be VBD cord-014901-d9szap94 105 25 revealed reveal VBN cord-014901-d9szap94 105 26 [ [ -LRB- cord-014901-d9szap94 105 27 25 25 CD cord-014901-d9szap94 105 28 ] ] -RRB- cord-014901-d9szap94 105 29 . . . cord-014901-d9szap94 106 1 Differ differ VBP cord-014901-d9szap94 106 2 ences ence NNS cord-014901-d9szap94 106 3 in in IN cord-014901-d9szap94 106 4 glycosylation glycosylation NN cord-014901-d9szap94 106 5 during during IN cord-014901-d9szap94 106 6 posttranslational posttranslational JJ cord-014901-d9szap94 106 7 modi modi NNP cord-014901-d9szap94 106 8 fications fication NNS cord-014901-d9szap94 106 9 of of IN cord-014901-d9szap94 106 10 recombinant recombinant JJ cord-014901-d9szap94 106 11 proteins protein NNS cord-014901-d9szap94 106 12 in in IN cord-014901-d9szap94 106 13 plant plant NN cord-014901-d9szap94 106 14 and and CC cord-014901-d9szap94 106 15 mam mam NN cord-014901-d9szap94 106 16 mal mal FW cord-014901-d9szap94 106 17 cells cell NNS cord-014901-d9szap94 106 18 can can MD cord-014901-d9szap94 106 19 be be VB cord-014901-d9szap94 106 20 eliminated eliminate VBN cord-014901-d9szap94 106 21 by by IN cord-014901-d9szap94 106 22 genetic genetic JJ cord-014901-d9szap94 106 23 engineering engineering NN cord-014901-d9szap94 106 24 techniques technique NNS cord-014901-d9szap94 106 25 , , , cord-014901-d9szap94 106 26 which which WDT cord-014901-d9szap94 106 27 was be VBD cord-014901-d9szap94 106 28 successfully successfully RB cord-014901-d9szap94 106 29 demonstrated demonstrate VBN cord-014901-d9szap94 106 30 on on IN cord-014901-d9szap94 106 31 the the DT cord-014901-d9szap94 106 32 moss moss NNP cord-014901-d9szap94 106 33 Physcomitrella Physcomitrella NNP cord-014901-d9szap94 106 34 patens paten NNS cord-014901-d9szap94 106 35 , , , cord-014901-d9szap94 106 36 in in IN cord-014901-d9szap94 106 37 which which WDT cord-014901-d9szap94 106 38 the the DT cord-014901-d9szap94 106 39 genes gene NNS cord-014901-d9szap94 106 40 encoding encode VBG cord-014901-d9szap94 106 41 the the DT cord-014901-d9szap94 106 42 enzymes enzyme NNS cord-014901-d9szap94 106 43 β β NN cord-014901-d9szap94 106 44 1,2 1,2 CD cord-014901-d9szap94 106 45 xylosyltransferase xylosyltransferase NN cord-014901-d9szap94 106 46 and and CC cord-014901-d9szap94 106 47 α α NN cord-014901-d9szap94 106 48 1,3 1,3 CD cord-014901-d9szap94 106 49 fucosyltransferase fucosyltransferase NN cord-014901-d9szap94 106 50 responsible responsible JJ cord-014901-d9szap94 106 51 , , , cord-014901-d9szap94 106 52 respectively respectively RB cord-014901-d9szap94 106 53 , , , cord-014901-d9szap94 106 54 for for IN cord-014901-d9szap94 106 55 xylosylation xylosylation NN cord-014901-d9szap94 106 56 and and CC cord-014901-d9szap94 106 57 fucosylation fucosylation NN cord-014901-d9szap94 106 58 of of IN cord-014901-d9szap94 106 59 proteins protein NNS cord-014901-d9szap94 106 60 were be VBD cord-014901-d9szap94 106 61 switched switch VBN cord-014901-d9szap94 106 62 off off RP cord-014901-d9szap94 106 63 by by IN cord-014901-d9szap94 106 64 the the DT cord-014901-d9szap94 106 65 " " `` cord-014901-d9szap94 106 66 knock knock VB cord-014901-d9szap94 106 67 down down RP cord-014901-d9szap94 106 68 " " '' cord-014901-d9szap94 106 69 method method NN cord-014901-d9szap94 106 70 [ [ -LRB- cord-014901-d9szap94 106 71 25 25 CD cord-014901-d9szap94 106 72 ] ] -RRB- cord-014901-d9szap94 106 73 . . . cord-014901-d9szap94 107 1 It -PRON- PRP cord-014901-d9szap94 107 2 is be VBZ cord-014901-d9szap94 107 3 known know VBN cord-014901-d9szap94 107 4 that that IN cord-014901-d9szap94 107 5 when when WRB cord-014901-d9szap94 107 6 the the DT cord-014901-d9szap94 107 7 foreign foreign JJ cord-014901-d9szap94 107 8 gene gene NN cord-014901-d9szap94 107 9 is be VBZ cord-014901-d9szap94 107 10 inserted insert VBN cord-014901-d9szap94 107 11 in in IN cord-014901-d9szap94 107 12 the the DT cord-014901-d9szap94 107 13 genome genome NN cord-014901-d9szap94 107 14 of of IN cord-014901-d9szap94 107 15 the the DT cord-014901-d9szap94 107 16 transgenic transgenic JJ cord-014901-d9szap94 107 17 plant plant NN cord-014901-d9szap94 107 18 , , , cord-014901-d9szap94 107 19 the the DT cord-014901-d9szap94 107 20 level level NN cord-014901-d9szap94 107 21 of of IN cord-014901-d9szap94 107 22 its -PRON- PRP$ cord-014901-d9szap94 107 23 expression expression NN cord-014901-d9szap94 107 24 depends depend VBZ cord-014901-d9szap94 107 25 on on IN cord-014901-d9szap94 107 26 the the DT cord-014901-d9szap94 107 27 site site NN cord-014901-d9szap94 107 28 of of IN cord-014901-d9szap94 107 29 its -PRON- PRP$ cord-014901-d9szap94 107 30 insertion insertion NN cord-014901-d9szap94 107 31 , , , cord-014901-d9szap94 107 32 which which WDT cord-014901-d9szap94 107 33 determines determine VBZ cord-014901-d9szap94 107 34 the the DT cord-014901-d9szap94 107 35 level level NN cord-014901-d9szap94 107 36 of of IN cord-014901-d9szap94 107 37 protein protein NN cord-014901-d9szap94 107 38 ( ( -LRB- cord-014901-d9szap94 107 39 antigen antigen NN cord-014901-d9szap94 107 40 in in IN cord-014901-d9szap94 107 41 particular particular JJ cord-014901-d9szap94 107 42 ) ) -RRB- cord-014901-d9szap94 107 43 accumulation accumulation NN cord-014901-d9szap94 107 44 in in IN cord-014901-d9szap94 107 45 plant plant NN cord-014901-d9szap94 107 46 tissues tissue NNS cord-014901-d9szap94 107 47 . . . cord-014901-d9szap94 108 1 In in IN cord-014901-d9szap94 108 2 this this DT cord-014901-d9szap94 108 3 connection connection NN cord-014901-d9szap94 108 4 , , , cord-014901-d9szap94 108 5 one one CD cord-014901-d9szap94 108 6 of of IN cord-014901-d9szap94 108 7 disadvantages disadvantage NNS cord-014901-d9szap94 108 8 of of IN cord-014901-d9szap94 108 9 plant plant NN cord-014901-d9szap94 108 10 vaccines vaccine NNS cord-014901-d9szap94 108 11 is be VBZ cord-014901-d9szap94 108 12 the the DT cord-014901-d9szap94 108 13 difficulty difficulty NN cord-014901-d9szap94 108 14 in in IN cord-014901-d9szap94 108 15 the the DT cord-014901-d9szap94 108 16 standardization standardization NN cord-014901-d9szap94 108 17 of of IN cord-014901-d9szap94 108 18 their -PRON- PRP$ cord-014901-d9szap94 108 19 dosage dosage NN cord-014901-d9szap94 108 20 . . . cord-014901-d9szap94 109 1 It -PRON- PRP cord-014901-d9szap94 109 2 is be VBZ cord-014901-d9szap94 109 3 at at IN cord-014901-d9szap94 109 4 this this DT cord-014901-d9szap94 109 5 stage stage NN cord-014901-d9szap94 109 6 the the DT cord-014901-d9szap94 109 7 development development NN cord-014901-d9szap94 109 8 of of IN cord-014901-d9szap94 109 9 the the DT cord-014901-d9szap94 109 10 " " `` cord-014901-d9szap94 109 11 edible edible JJ cord-014901-d9szap94 109 12 vaccine vaccine NN cord-014901-d9szap94 109 13 " " '' cord-014901-d9szap94 109 14 concept concept NN cord-014901-d9szap94 109 15 has have VBZ cord-014901-d9szap94 109 16 undergone undergo VBN cord-014901-d9szap94 109 17 substantial substantial JJ cord-014901-d9szap94 109 18 revision revision NN cord-014901-d9szap94 109 19 , , , cord-014901-d9szap94 109 20 since since IN cord-014901-d9szap94 109 21 the the DT cord-014901-d9szap94 109 22 possibility possibility NN cord-014901-d9szap94 109 23 of of IN cord-014901-d9szap94 109 24 oral oral JJ cord-014901-d9szap94 109 25 delivery delivery NN cord-014901-d9szap94 109 26 of of IN cord-014901-d9szap94 109 27 the the DT cord-014901-d9szap94 109 28 recombinant recombinant JJ cord-014901-d9szap94 109 29 antigen antigen NN cord-014901-d9szap94 109 30 with with IN cord-014901-d9szap94 109 31 the the DT cord-014901-d9szap94 109 32 raw raw JJ cord-014901-d9szap94 109 33 plant plant NN cord-014901-d9szap94 109 34 material material NN cord-014901-d9szap94 109 35 was be VBD cord-014901-d9szap94 109 36 untenable untenable JJ cord-014901-d9szap94 109 37 because because IN cord-014901-d9szap94 109 38 of of IN cord-014901-d9szap94 109 39 the the DT cord-014901-d9szap94 109 40 variability variability NN cord-014901-d9szap94 109 41 of of IN cord-014901-d9szap94 109 42 the the DT cord-014901-d9szap94 109 43 recombinant recombinant JJ cord-014901-d9szap94 109 44 protein protein NN cord-014901-d9szap94 109 45 content content NN cord-014901-d9szap94 109 46 . . . cord-014901-d9szap94 110 1 According accord VBG cord-014901-d9szap94 110 2 to to IN cord-014901-d9szap94 110 3 the the DT cord-014901-d9szap94 110 4 researchers researcher NNS cord-014901-d9szap94 110 5 involved involve VBN cord-014901-d9szap94 110 6 in in IN cord-014901-d9szap94 110 7 the the DT cord-014901-d9szap94 110 8 development development NN cord-014901-d9szap94 110 9 of of IN cord-014901-d9szap94 110 10 " " `` cord-014901-d9szap94 110 11 candi candi NNP cord-014901-d9szap94 110 12 date date NN cord-014901-d9szap94 110 13 " " '' cord-014901-d9szap94 110 14 plant plant NN cord-014901-d9szap94 110 15 vaccines vaccine NNS cord-014901-d9szap94 110 16 , , , cord-014901-d9szap94 110 17 the the DT cord-014901-d9szap94 110 18 antigen antigen NN cord-014901-d9szap94 110 19 dosage dosage NN cord-014901-d9szap94 110 20 problem problem NN cord-014901-d9szap94 110 21 in in IN cord-014901-d9szap94 110 22 plant plant NN cord-014901-d9szap94 110 23 tissues tissue NNS cord-014901-d9szap94 110 24 can can MD cord-014901-d9szap94 110 25 be be VB cord-014901-d9szap94 110 26 successfully successfully RB cord-014901-d9szap94 110 27 solved solve VBN cord-014901-d9szap94 110 28 by by IN cord-014901-d9szap94 110 29 the the DT cord-014901-d9szap94 110 30 intro intro NNP cord-014901-d9szap94 110 31 duction duction NN cord-014901-d9szap94 110 32 of of IN cord-014901-d9szap94 110 33 additional additional JJ cord-014901-d9szap94 110 34 treatment treatment NN cord-014901-d9szap94 110 35 of of IN cord-014901-d9szap94 110 36 the the DT cord-014901-d9szap94 110 37 plant plant NN cord-014901-d9szap94 110 38 material material NN cord-014901-d9szap94 110 39 : : : cord-014901-d9szap94 110 40 its -PRON- PRP$ cord-014901-d9szap94 110 41 refinement refinement NN cord-014901-d9szap94 110 42 ( ( -LRB- cord-014901-d9szap94 110 43 to to TO cord-014901-d9szap94 110 44 equalize equalize VB cord-014901-d9szap94 110 45 the the DT cord-014901-d9szap94 110 46 concentration concentration NN cord-014901-d9szap94 110 47 of of IN cord-014901-d9szap94 110 48 the the DT cord-014901-d9szap94 110 49 antigen antigen NN cord-014901-d9szap94 110 50 ) ) -RRB- cord-014901-d9szap94 110 51 , , , cord-014901-d9szap94 110 52 drying drying NN cord-014901-d9szap94 110 53 or or CC cord-014901-d9szap94 110 54 lyophilization lyophilization NN cord-014901-d9szap94 110 55 . . . cord-014901-d9szap94 111 1 It -PRON- PRP cord-014901-d9szap94 111 2 is be VBZ cord-014901-d9szap94 111 3 also also RB cord-014901-d9szap94 111 4 necessary necessary JJ cord-014901-d9szap94 111 5 to to TO cord-014901-d9szap94 111 6 introduce introduce VB cord-014901-d9szap94 111 7 an an DT cord-014901-d9szap94 111 8 additional additional JJ cord-014901-d9szap94 111 9 stage stage NN cord-014901-d9szap94 111 10 associated associate VBN cord-014901-d9szap94 111 11 with with IN cord-014901-d9szap94 111 12 the the DT cord-014901-d9szap94 111 13 development development NN cord-014901-d9szap94 111 14 of of IN cord-014901-d9szap94 111 15 rapid rapid JJ cord-014901-d9szap94 111 16 methods method NNS cord-014901-d9szap94 111 17 for for IN cord-014901-d9szap94 111 18 determining determine VBG cord-014901-d9szap94 111 19 and and CC cord-014901-d9szap94 111 20 monitoring monitor VBG cord-014901-d9szap94 111 21 the the DT cord-014901-d9szap94 111 22 dosage dosage NN cord-014901-d9szap94 111 23 of of IN cord-014901-d9szap94 111 24 the the DT cord-014901-d9szap94 111 25 recombinant recombinant JJ cord-014901-d9szap94 111 26 antigen antigen NN cord-014901-d9szap94 111 27 . . . cord-014901-d9szap94 112 1 After after IN cord-014901-d9szap94 112 2 appropriate appropriate JJ cord-014901-d9szap94 112 3 preparation preparation NN cord-014901-d9szap94 112 4 , , , cord-014901-d9szap94 112 5 plant plant NN cord-014901-d9szap94 112 6 vaccines vaccine NNS cord-014901-d9szap94 112 7 can can MD cord-014901-d9szap94 112 8 be be VB cord-014901-d9szap94 112 9 encapsulated encapsulate VBN cord-014901-d9szap94 112 10 , , , cord-014901-d9szap94 112 11 tableted tableted JJ cord-014901-d9szap94 112 12 , , , cord-014901-d9szap94 112 13 and and CC cord-014901-d9szap94 112 14 used use VBN cord-014901-d9szap94 112 15 in in IN cord-014901-d9szap94 112 16 practice practice NN cord-014901-d9szap94 112 17 under under IN cord-014901-d9szap94 112 18 corresponding correspond VBG cord-014901-d9szap94 112 19 medical medical JJ cord-014901-d9szap94 112 20 supervision supervision NN cord-014901-d9szap94 112 21 [ [ -LRB- cord-014901-d9szap94 112 22 14 14 CD cord-014901-d9szap94 112 23 , , , cord-014901-d9szap94 112 24 26 26 CD cord-014901-d9szap94 112 25 ] ] -RRB- cord-014901-d9szap94 112 26 . . . cord-014901-d9szap94 113 1 Thus thus RB cord-014901-d9szap94 113 2 , , , cord-014901-d9szap94 113 3 performed perform VBN cord-014901-d9szap94 113 4 studies study NNS cord-014901-d9szap94 113 5 allow allow VBP cord-014901-d9szap94 113 6 a a DT cord-014901-d9szap94 113 7 suggestion suggestion NN cord-014901-d9szap94 113 8 that that IN cord-014901-d9szap94 113 9 the the DT cord-014901-d9szap94 113 10 plant plant NN cord-014901-d9szap94 113 11 vaccine vaccine NN cord-014901-d9szap94 113 12 based base VBN cord-014901-d9szap94 113 13 on on IN cord-014901-d9szap94 113 14 the the DT cord-014901-d9szap94 113 15 genetically genetically RB cord-014901-d9szap94 113 16 modified modify VBN cord-014901-d9szap94 113 17 plants plant NNS cord-014901-d9szap94 113 18 is be VBZ cord-014901-d9szap94 113 19 capable capable JJ cord-014901-d9szap94 113 20 of of IN cord-014901-d9szap94 113 21 inducing induce VBG cord-014901-d9szap94 113 22 protective protective JJ cord-014901-d9szap94 113 23 immunity immunity NN cord-014901-d9szap94 113 24 and and CC cord-014901-d9szap94 113 25 open open JJ cord-014901-d9szap94 113 26 new new JJ cord-014901-d9szap94 113 27 opportunities opportunity NNS cord-014901-d9szap94 113 28 for for IN cord-014901-d9szap94 113 29 the the DT cord-014901-d9szap94 113 30 creation creation NN cord-014901-d9szap94 113 31 of of IN cord-014901-d9szap94 113 32 low low JJ cord-014901-d9szap94 113 33 cost cost NN cord-014901-d9szap94 113 34 and and CC cord-014901-d9szap94 113 35 easy easy JJ cord-014901-d9szap94 113 36 handling handle VBG cord-014901-d9szap94 113 37 vaccines vaccine NNS cord-014901-d9szap94 113 38 against against IN cord-014901-d9szap94 113 39 infectious infectious JJ cord-014901-d9szap94 113 40 diseases disease NNS cord-014901-d9szap94 113 41 of of IN cord-014901-d9szap94 113 42 animals animal NNS cord-014901-d9szap94 113 43 . . . cord-014901-d9szap94 114 1 It -PRON- PRP cord-014901-d9szap94 114 2 is be VBZ cord-014901-d9szap94 114 3 of of IN cord-014901-d9szap94 114 4 importance importance NN cord-014901-d9szap94 114 5 that that WDT cord-014901-d9szap94 114 6 developed develop VBD cord-014901-d9szap94 114 7 to to IN cord-014901-d9szap94 114 8 date date NN cord-014901-d9szap94 114 9 , , , cord-014901-d9szap94 114 10 highly highly RB cord-014901-d9szap94 114 11 effective effective JJ cord-014901-d9szap94 114 12 methods method NNS cord-014901-d9szap94 114 13 of of IN cord-014901-d9szap94 114 14 cultivation cultivation NN cord-014901-d9szap94 114 15 of of IN cord-014901-d9szap94 114 16 agro agro JJ cord-014901-d9szap94 114 17 eco eco JJ cord-014901-d9szap94 114 18 nomically nomically RB cord-014901-d9szap94 114 19 important important JJ cord-014901-d9szap94 114 20 plant plant NN cord-014901-d9szap94 114 21 species specie NNS cord-014901-d9szap94 114 22 , , , cord-014901-d9szap94 114 23 as as RB cord-014901-d9szap94 114 24 well well RB cord-014901-d9szap94 114 25 as as IN cord-014901-d9szap94 114 26 the the DT cord-014901-d9szap94 114 27 seed seed NN cord-014901-d9szap94 114 28 production production NN cord-014901-d9szap94 114 29 system system NN cord-014901-d9szap94 114 30 for for IN cord-014901-d9szap94 114 31 a a DT cord-014901-d9szap94 114 32 particular particular JJ cord-014901-d9szap94 114 33 culture culture NN cord-014901-d9szap94 114 34 make make VBP cord-014901-d9szap94 114 35 the the DT cord-014901-d9szap94 114 36 plants plant NNS cord-014901-d9szap94 114 37 attractive attractive JJ cord-014901-d9szap94 114 38 to to TO cord-014901-d9szap94 114 39 be be VB cord-014901-d9szap94 114 40 used use VBN cord-014901-d9szap94 114 41 as as IN cord-014901-d9szap94 114 42 " " `` cord-014901-d9szap94 114 43 biofactories biofactorie NNS cord-014901-d9szap94 114 44 " " '' cord-014901-d9szap94 114 45 for for IN cord-014901-d9szap94 114 46 man man NN cord-014901-d9szap94 114 47 ufacturing ufacture VBG cord-014901-d9szap94 114 48 cheap cheap JJ cord-014901-d9szap94 114 49 recombinant recombinant JJ cord-014901-d9szap94 114 50 proteins protein NNS cord-014901-d9szap94 114 51 for for IN cord-014901-d9szap94 114 52 medical medical JJ cord-014901-d9szap94 114 53 purposes purpose NNS cord-014901-d9szap94 114 54 . . . cord-014901-d9szap94 115 1 roplasts roplasts NNP cord-014901-d9szap94 115 2 ) ) -RRB- cord-014901-d9szap94 115 3 . . . cord-014901-d9szap94 116 1 Among among IN cord-014901-d9szap94 116 2 plant plant NN cord-014901-d9szap94 116 3 expression expression NN cord-014901-d9szap94 116 4 systems system NNS cord-014901-d9szap94 116 5 with with IN cord-014901-d9szap94 116 6 stable stable JJ cord-014901-d9szap94 116 7 integration integration NN cord-014901-d9szap94 116 8 of of IN cord-014901-d9szap94 116 9 the the DT cord-014901-d9szap94 116 10 transgene transgene NN cord-014901-d9szap94 116 11 in in IN cord-014901-d9szap94 116 12 the the DT cord-014901-d9szap94 116 13 nuclear nuclear JJ cord-014901-d9szap94 116 14 genome genome NN cord-014901-d9szap94 116 15 , , , cord-014901-d9szap94 116 16 a a DT cord-014901-d9szap94 116 17 separate separate JJ cord-014901-d9szap94 116 18 group group NN cord-014901-d9szap94 116 19 includes include VBZ cord-014901-d9szap94 116 20 the the DT cord-014901-d9szap94 116 21 cultivated cultivate VBN cord-014901-d9szap94 116 22 duckweed duckweed NN cord-014901-d9szap94 116 23 , , , cord-014901-d9szap94 116 24 microalgae microalgae NN cord-014901-d9szap94 116 25 , , , cord-014901-d9szap94 116 26 and and CC cord-014901-d9szap94 116 27 cell cell NN cord-014901-d9szap94 116 28 culture culture NN cord-014901-d9szap94 116 29 systems system NNS cord-014901-d9szap94 116 30 in in IN cord-014901-d9szap94 116 31 vitro vitro FW cord-014901-d9szap94 116 32 : : : cord-014901-d9szap94 116 33 cell cell NN cord-014901-d9szap94 116 34 sus sus NNP cord-014901-d9szap94 116 35 pensions pension NNS cord-014901-d9szap94 116 36 , , , cord-014901-d9szap94 116 37 cultures culture NNS cord-014901-d9szap94 116 38 of of IN cord-014901-d9szap94 116 39 " " `` cord-014901-d9szap94 116 40 hairy hairy JJ cord-014901-d9szap94 116 41 roots root NNS cord-014901-d9szap94 116 42 " " '' cord-014901-d9szap94 116 43 , , , cord-014901-d9szap94 116 44 moss moss NN cord-014901-d9szap94 116 45 protonemas protonemas NNP cord-014901-d9szap94 116 46 , , , cord-014901-d9szap94 116 47 which which WDT cord-014901-d9szap94 116 48 cultivation cultivation NN cord-014901-d9szap94 116 49 conditions condition NNS cord-014901-d9szap94 116 50 are be VBP cord-014901-d9szap94 116 51 a a DT cord-014901-d9szap94 116 52 completely completely RB cord-014901-d9szap94 116 53 closed close VBN cord-014901-d9szap94 116 54 environment environment NN cord-014901-d9szap94 116 55 ( ( -LRB- cord-014901-d9szap94 116 56 bioreactors bioreactor NNS cord-014901-d9szap94 116 57 ) ) -RRB- cord-014901-d9szap94 116 58 . . . cord-014901-d9szap94 117 1 Promising promise VBG cord-014901-d9szap94 117 2 is be VBZ cord-014901-d9szap94 117 3 the the DT cord-014901-d9szap94 117 4 transient transient JJ cord-014901-d9szap94 117 5 expression expression NN cord-014901-d9szap94 117 6 system system NN cord-014901-d9szap94 117 7 , , , cord-014901-d9szap94 117 8 in in IN cord-014901-d9szap94 117 9 which which WDT cord-014901-d9szap94 117 10 the the DT cord-014901-d9szap94 117 11 target target NN cord-014901-d9szap94 117 12 gene gene NN cord-014901-d9szap94 117 13 is be VBZ cord-014901-d9szap94 117 14 intro intro NNP cord-014901-d9szap94 117 15 duced duce VBN cord-014901-d9szap94 117 16 into into IN cord-014901-d9szap94 117 17 plant plant NN cord-014901-d9szap94 117 18 cells cell NNS cord-014901-d9szap94 117 19 and and CC cord-014901-d9szap94 117 20 is be VBZ cord-014901-d9szap94 117 21 expressed express VBN cord-014901-d9szap94 117 22 for for IN cord-014901-d9szap94 117 23 a a DT cord-014901-d9szap94 117 24 short short JJ cord-014901-d9szap94 117 25 period period NN cord-014901-d9szap94 117 26 of of IN cord-014901-d9szap94 117 27 time time NN cord-014901-d9szap94 117 28 ( ( -LRB- cord-014901-d9szap94 117 29 several several JJ cord-014901-d9szap94 117 30 days day NNS cord-014901-d9szap94 117 31 ) ) -RRB- cord-014901-d9szap94 117 32 , , , cord-014901-d9szap94 117 33 but but CC cord-014901-d9szap94 117 34 is be VBZ cord-014901-d9szap94 117 35 not not RB cord-014901-d9szap94 117 36 integrated integrate VBN cord-014901-d9szap94 117 37 into into IN cord-014901-d9szap94 117 38 the the DT cord-014901-d9szap94 117 39 genome genome NN cord-014901-d9szap94 117 40 . . . cord-014901-d9szap94 118 1 Each each DT cord-014901-d9szap94 118 2 of of IN cord-014901-d9szap94 118 3 these these DT cord-014901-d9szap94 118 4 expression expression NN cord-014901-d9szap94 118 5 systems system NNS cord-014901-d9szap94 118 6 has have VBZ cord-014901-d9szap94 118 7 its -PRON- PRP$ cord-014901-d9szap94 118 8 advantages advantage NNS cord-014901-d9szap94 118 9 and and CC cord-014901-d9szap94 118 10 disadvantages disadvantage NNS cord-014901-d9szap94 118 11 ; ; : cord-014901-d9szap94 118 12 the the DT cord-014901-d9szap94 118 13 main main JJ cord-014901-d9szap94 118 14 details detail NNS cord-014901-d9szap94 118 15 of of IN cord-014901-d9szap94 118 16 these these DT cord-014901-d9szap94 118 17 systems system NNS cord-014901-d9szap94 118 18 are be VBP cord-014901-d9szap94 118 19 considered consider VBN cord-014901-d9szap94 118 20 below below RB cord-014901-d9szap94 118 21 . . . cord-014901-d9szap94 119 1 The the DT cord-014901-d9szap94 119 2 most most RBS cord-014901-d9szap94 119 3 widely widely RB cord-014901-d9szap94 119 4 used use VBN cord-014901-d9szap94 119 5 system system NN cord-014901-d9szap94 119 6 of of IN cord-014901-d9szap94 119 7 heterologous heterologous JJ cord-014901-d9szap94 119 8 gene gene NN cord-014901-d9szap94 119 9 expression expression NN cord-014901-d9szap94 119 10 , , , cord-014901-d9szap94 119 11 in in IN cord-014901-d9szap94 119 12 particular particular JJ cord-014901-d9szap94 119 13 for for IN cord-014901-d9szap94 119 14 plant plant NN cord-014901-d9szap94 119 15 vaccine vaccine NN cord-014901-d9szap94 119 16 production production NN cord-014901-d9szap94 119 17 , , , cord-014901-d9szap94 119 18 are be VBP cord-014901-d9szap94 119 19 transgenic transgenic JJ cord-014901-d9szap94 119 20 plants plant NNS cord-014901-d9szap94 119 21 with with IN cord-014901-d9szap94 119 22 the the DT cord-014901-d9szap94 119 23 stable stable JJ cord-014901-d9szap94 119 24 integration integration NN cord-014901-d9szap94 119 25 of of IN cord-014901-d9szap94 119 26 the the DT cord-014901-d9szap94 119 27 transgene transgene NN cord-014901-d9szap94 119 28 into into IN cord-014901-d9szap94 119 29 the the DT cord-014901-d9szap94 119 30 nuclear nuclear JJ cord-014901-d9szap94 119 31 genome genome NN cord-014901-d9szap94 119 32 . . . cord-014901-d9szap94 120 1 The the DT cord-014901-d9szap94 120 2 creation creation NN cord-014901-d9szap94 120 3 of of IN cord-014901-d9szap94 120 4 these these DT cord-014901-d9szap94 120 5 plants plant NNS cord-014901-d9szap94 120 6 involves involve VBZ cord-014901-d9szap94 120 7 the the DT cord-014901-d9szap94 120 8 transfer transfer NN cord-014901-d9szap94 120 9 of of IN cord-014901-d9szap94 120 10 foreign foreign JJ cord-014901-d9szap94 120 11 genes gene NNS cord-014901-d9szap94 120 12 into into IN cord-014901-d9szap94 120 13 the the DT cord-014901-d9szap94 120 14 genome genome NN cord-014901-d9szap94 120 15 of of IN cord-014901-d9szap94 120 16 plant plant NN cord-014901-d9szap94 120 17 cells cell NNS cord-014901-d9szap94 120 18 using use VBG cord-014901-d9szap94 120 19 Agrobacterium Agrobacterium NNP cord-014901-d9szap94 120 20 tumefa tumefa XX cord-014901-d9szap94 120 21 ciens ciens NNP cord-014901-d9szap94 120 22 or or CC cord-014901-d9szap94 120 23 bioballistics bioballistic NNS cord-014901-d9szap94 120 24 and and CC cord-014901-d9szap94 120 25 subsequent subsequent JJ cord-014901-d9szap94 120 26 regeneration regeneration NN cord-014901-d9szap94 120 27 of of IN cord-014901-d9szap94 120 28 transformed transform VBN cord-014901-d9szap94 120 29 plants plant NNS cord-014901-d9szap94 120 30 from from IN cord-014901-d9szap94 120 31 these these DT cord-014901-d9szap94 120 32 cells cell NNS cord-014901-d9szap94 120 33 . . . cord-014901-d9szap94 121 1 Being be VBG cord-014901-d9szap94 121 2 inserted insert VBN cord-014901-d9szap94 121 3 into into IN cord-014901-d9szap94 121 4 the the DT cord-014901-d9szap94 121 5 nuclear nuclear JJ cord-014901-d9szap94 121 6 genome genome NN cord-014901-d9szap94 121 7 , , , cord-014901-d9szap94 121 8 transgene transgene NN cord-014901-d9szap94 121 9 becomes become VBZ cord-014901-d9szap94 121 10 its -PRON- PRP$ cord-014901-d9szap94 121 11 resi resi JJ cord-014901-d9szap94 121 12 dent dent NN cord-014901-d9szap94 121 13 part part NN cord-014901-d9szap94 121 14 , , , cord-014901-d9szap94 121 15 is be VBZ cord-014901-d9szap94 121 16 stably stably RB cord-014901-d9szap94 121 17 expressed express VBN cord-014901-d9szap94 121 18 , , , cord-014901-d9szap94 121 19 and and CC cord-014901-d9szap94 121 20 is be VBZ cord-014901-d9szap94 121 21 maintained maintain VBN cord-014901-d9szap94 121 22 in in IN cord-014901-d9szap94 121 23 subsequent subsequent JJ cord-014901-d9szap94 121 24 generations generation NNS cord-014901-d9szap94 121 25 . . . cord-014901-d9szap94 122 1 Using use VBG cord-014901-d9szap94 122 2 this this DT cord-014901-d9szap94 122 3 expression expression NN cord-014901-d9szap94 122 4 system system NN cord-014901-d9szap94 122 5 for for IN cord-014901-d9szap94 122 6 producing produce VBG cord-014901-d9szap94 122 7 recom recom NNP cord-014901-d9szap94 122 8 binant binant JJ cord-014901-d9szap94 122 9 proteins protein NNS cord-014901-d9szap94 122 10 , , , cord-014901-d9szap94 122 11 including include VBG cord-014901-d9szap94 122 12 plant plant NN cord-014901-d9szap94 122 13 vaccines vaccine NNS cord-014901-d9szap94 122 14 , , , cord-014901-d9szap94 122 15 is be VBZ cord-014901-d9szap94 122 16 still still RB cord-014901-d9szap94 122 17 ham ham VBN cord-014901-d9szap94 122 18 pered pere VBN cord-014901-d9szap94 122 19 by by IN cord-014901-d9szap94 122 20 relatively relatively RB cord-014901-d9szap94 122 21 low low JJ cord-014901-d9szap94 122 22 levels level NNS cord-014901-d9szap94 122 23 of of IN cord-014901-d9szap94 122 24 transgene transgene NN cord-014901-d9szap94 122 25 expression expression NN cord-014901-d9szap94 122 26 , , , cord-014901-d9szap94 122 27 which which WDT cord-014901-d9szap94 122 28 is be VBZ cord-014901-d9szap94 122 29 , , , cord-014901-d9szap94 122 30 as as IN cord-014901-d9szap94 122 31 a a DT cord-014901-d9szap94 122 32 rule rule NN cord-014901-d9szap94 122 33 , , , cord-014901-d9szap94 122 34 less less JJR cord-014901-d9szap94 122 35 than than IN cord-014901-d9szap94 122 36 1 1 CD cord-014901-d9szap94 122 37 % % NN cord-014901-d9szap94 122 38 of of IN cord-014901-d9szap94 122 39 total total JJ cord-014901-d9szap94 122 40 soluble soluble JJ cord-014901-d9szap94 122 41 protein protein NN cord-014901-d9szap94 122 42 ( ( -LRB- cord-014901-d9szap94 122 43 TSP TSP NNP cord-014901-d9szap94 122 44 ) ) -RRB- cord-014901-d9szap94 122 45 and and CC cord-014901-d9szap94 122 46 also also RB cord-014901-d9szap94 122 47 by by IN cord-014901-d9szap94 122 48 its -PRON- PRP$ cord-014901-d9szap94 122 49 variability variability NN cord-014901-d9szap94 122 50 in in IN cord-014901-d9szap94 122 51 different different JJ cord-014901-d9szap94 122 52 plant plant NN cord-014901-d9szap94 122 53 organs organ NNS cord-014901-d9szap94 122 54 and and CC cord-014901-d9szap94 122 55 tissues tissue NNS cord-014901-d9szap94 122 56 within within IN cord-014901-d9szap94 122 57 a a DT cord-014901-d9szap94 122 58 single single JJ cord-014901-d9szap94 122 59 plant plant NN cord-014901-d9szap94 122 60 and and CC cord-014901-d9szap94 122 61 in in IN cord-014901-d9szap94 122 62 different different JJ cord-014901-d9szap94 122 63 plants plant NNS cord-014901-d9szap94 122 64 . . . cord-014901-d9szap94 123 1 Most most RBS cord-014901-d9szap94 123 2 often often RB cord-014901-d9szap94 123 3 , , , cord-014901-d9szap94 123 4 the the DT cord-014901-d9szap94 123 5 variability variability NN cord-014901-d9szap94 123 6 in in IN cord-014901-d9szap94 123 7 expression expression NN cord-014901-d9szap94 123 8 of of IN cord-014901-d9szap94 123 9 the the DT cord-014901-d9szap94 123 10 transgene transgene NN cord-014901-d9szap94 123 11 is be VBZ cord-014901-d9szap94 123 12 due due JJ cord-014901-d9szap94 123 13 to to IN cord-014901-d9szap94 123 14 the the DT cord-014901-d9szap94 123 15 random random JJ cord-014901-d9szap94 123 16 nature nature NN cord-014901-d9szap94 123 17 of of IN cord-014901-d9szap94 123 18 its -PRON- PRP$ cord-014901-d9szap94 123 19 insertion insertion NN cord-014901-d9szap94 123 20 into into IN cord-014901-d9szap94 123 21 the the DT cord-014901-d9szap94 123 22 nuclear nuclear JJ cord-014901-d9szap94 123 23 genome genome NN cord-014901-d9szap94 123 24 ( ( -LRB- cord-014901-d9szap94 123 25 effect effect NN cord-014901-d9szap94 123 26 of of IN cord-014901-d9szap94 123 27 position position NN cord-014901-d9szap94 123 28 ) ) -RRB- cord-014901-d9szap94 123 29 and and CC cord-014901-d9szap94 123 30 may may MD cord-014901-d9szap94 123 31 be be VB cord-014901-d9szap94 123 32 associ associ NNP cord-014901-d9szap94 123 33 ated ate VBN cord-014901-d9szap94 123 34 with with IN cord-014901-d9szap94 123 35 partial partial JJ cord-014901-d9szap94 123 36 or or CC cord-014901-d9szap94 123 37 complete complete JJ cord-014901-d9szap94 123 38 transgene transgene NN cord-014901-d9szap94 123 39 inactivation inactivation NN cord-014901-d9szap94 123 40 [ [ -LRB- cord-014901-d9szap94 123 41 27 27 CD cord-014901-d9szap94 123 42 , , , cord-014901-d9szap94 123 43 28 28 CD cord-014901-d9szap94 123 44 ] ] -RRB- cord-014901-d9szap94 123 45 . . . cord-014901-d9szap94 124 1 Experts expert NNS cord-014901-d9szap94 124 2 believe believe VBP cord-014901-d9szap94 124 3 that that IN cord-014901-d9szap94 124 4 the the DT cord-014901-d9szap94 124 5 use use NN cord-014901-d9szap94 124 6 of of IN cord-014901-d9szap94 124 7 plant plant NN cord-014901-d9szap94 124 8 expression expression NN cord-014901-d9szap94 124 9 sys sys NN cord-014901-d9szap94 124 10 tems tem NNS cord-014901-d9szap94 124 11 for for IN cord-014901-d9szap94 124 12 obtaining obtain VBG cord-014901-d9szap94 124 13 plant plant NN cord-014901-d9szap94 124 14 vaccines vaccine NNS cord-014901-d9szap94 124 15 is be VBZ cord-014901-d9szap94 124 16 economically economically RB cord-014901-d9szap94 124 17 bene bene VBN cord-014901-d9szap94 124 18 ficial ficial JJ cord-014901-d9szap94 124 19 at at IN cord-014901-d9szap94 124 20 the the DT cord-014901-d9szap94 124 21 level level NN cord-014901-d9szap94 124 22 of of IN cord-014901-d9szap94 124 23 expression expression NN cord-014901-d9szap94 124 24 of of IN cord-014901-d9szap94 124 25 a a DT cord-014901-d9szap94 124 26 target target NN cord-014901-d9szap94 124 27 gene gene NN cord-014901-d9szap94 124 28 , , , cord-014901-d9szap94 124 29 which which WDT cord-014901-d9szap94 124 30 allows allow VBZ cord-014901-d9szap94 124 31 the the DT cord-014901-d9szap94 124 32 accumulation accumulation NN cord-014901-d9szap94 124 33 of of IN cord-014901-d9szap94 124 34 recombinant recombinant JJ cord-014901-d9szap94 124 35 protein protein NN cord-014901-d9szap94 124 36 in in IN cord-014901-d9szap94 124 37 an an DT cord-014901-d9szap94 124 38 amount amount NN cord-014901-d9szap94 124 39 not not RB cord-014901-d9szap94 124 40 less less JJR cord-014901-d9szap94 124 41 than than IN cord-014901-d9szap94 124 42 1 1 CD cord-014901-d9szap94 124 43 % % NN cord-014901-d9szap94 124 44 of of IN cord-014901-d9szap94 124 45 TSP tsp NN cord-014901-d9szap94 124 46 [ [ -LRB- cord-014901-d9szap94 124 47 29 29 CD cord-014901-d9szap94 124 48 ] ] -RRB- cord-014901-d9szap94 124 49 . . . cord-014901-d9szap94 125 1 A a DT cord-014901-d9szap94 125 2 lot lot NN cord-014901-d9szap94 125 3 of of IN cord-014901-d9szap94 125 4 ways way NNS cord-014901-d9szap94 125 5 to to TO cord-014901-d9szap94 125 6 increase increase VB cord-014901-d9szap94 125 7 the the DT cord-014901-d9szap94 125 8 level level NN cord-014901-d9szap94 125 9 of of IN cord-014901-d9szap94 125 10 foreign foreign JJ cord-014901-d9szap94 125 11 gene gene NN cord-014901-d9szap94 125 12 expression expression NN cord-014901-d9szap94 125 13 in in IN cord-014901-d9szap94 125 14 transgenic transgenic JJ cord-014901-d9szap94 125 15 plants plant NNS cord-014901-d9szap94 125 16 is be VBZ cord-014901-d9szap94 125 17 developed develop VBN cord-014901-d9szap94 125 18 ; ; : cord-014901-d9szap94 125 19 they -PRON- PRP cord-014901-d9szap94 125 20 are be VBP cord-014901-d9szap94 125 21 very very RB cord-014901-d9szap94 125 22 fully fully RB cord-014901-d9szap94 125 23 discussed discuss VBN cord-014901-d9szap94 125 24 in in IN cord-014901-d9szap94 125 25 the the DT cord-014901-d9szap94 125 26 reviews review NNS cord-014901-d9szap94 125 27 [ [ -LRB- cord-014901-d9szap94 125 28 17 17 CD cord-014901-d9szap94 125 29 , , , cord-014901-d9szap94 125 30 30 30 CD cord-014901-d9szap94 125 31 , , , cord-014901-d9szap94 125 32 31 31 CD cord-014901-d9szap94 125 33 ] ] -RRB- cord-014901-d9szap94 125 34 . . . cord-014901-d9szap94 126 1 Among among IN cord-014901-d9szap94 126 2 them -PRON- PRP cord-014901-d9szap94 126 3 are be VBP cord-014901-d9szap94 126 4 the the DT cord-014901-d9szap94 126 5 optimization optimization NN cord-014901-d9szap94 126 6 of of IN cord-014901-d9szap94 126 7 the the DT cord-014901-d9szap94 126 8 codon codon NN cord-014901-d9szap94 126 9 composition composition NN cord-014901-d9szap94 126 10 of of IN cord-014901-d9szap94 126 11 the the DT cord-014901-d9szap94 126 12 target target NN cord-014901-d9szap94 126 13 sequence sequence NN cord-014901-d9szap94 126 14 , , , cord-014901-d9szap94 126 15 the the DT cord-014901-d9szap94 126 16 usage usage NN cord-014901-d9szap94 126 17 of of IN cord-014901-d9szap94 126 18 strong strong JJ cord-014901-d9szap94 126 19 promoters promoter NNS cord-014901-d9szap94 126 20 , , , cord-014901-d9szap94 126 21 the the DT cord-014901-d9szap94 126 22 addition addition NN cord-014901-d9szap94 126 23 of of IN cord-014901-d9szap94 126 24 introns intron NNS cord-014901-d9szap94 126 25 or or CC cord-014901-d9szap94 126 26 regions region NNS cord-014901-d9szap94 126 27 of of IN cord-014901-d9szap94 126 28 binding bind VBG cord-014901-d9szap94 126 29 with with IN cord-014901-d9szap94 126 30 the the DT cord-014901-d9szap94 126 31 nuclear nuclear JJ cord-014901-d9szap94 126 32 matrix matrix NN cord-014901-d9szap94 126 33 ( ( -LRB- cord-014901-d9szap94 126 34 SAR SAR NNP cord-014901-d9szap94 126 35 ) ) -RRB- cord-014901-d9szap94 126 36 , , , cord-014901-d9szap94 126 37 and and CC cord-014901-d9szap94 126 38 others other NNS cord-014901-d9szap94 126 39 . . . cord-014901-d9szap94 127 1 The the DT cord-014901-d9szap94 127 2 search search NN cord-014901-d9szap94 127 3 for for IN cord-014901-d9szap94 127 4 tis tis NNP cord-014901-d9szap94 127 5 sue sue NNP cord-014901-d9szap94 127 6 and and CC cord-014901-d9szap94 127 7 organ organ NN cord-014901-d9szap94 127 8 specific specific JJ cord-014901-d9szap94 127 9 promoters promoter NNS cord-014901-d9szap94 127 10 , , , cord-014901-d9szap94 127 11 i.e. i.e. FW cord-014901-d9szap94 127 12 , , , cord-014901-d9szap94 127 13 promoters promoter NNS cord-014901-d9szap94 127 14 providing provide VBG cord-014901-d9szap94 127 15 for for IN cord-014901-d9szap94 127 16 target target NN cord-014901-d9szap94 127 17 gene gene NN cord-014901-d9szap94 127 18 expression expression NN cord-014901-d9szap94 127 19 in in IN cord-014901-d9szap94 127 20 definite definite JJ cord-014901-d9szap94 127 21 plant plant NN cord-014901-d9szap94 127 22 tissues tissue NNS cord-014901-d9szap94 127 23 or or CC cord-014901-d9szap94 127 24 organs organ NNS cord-014901-d9szap94 127 25 , , , cord-014901-d9szap94 127 26 is be VBZ cord-014901-d9szap94 127 27 of of IN cord-014901-d9szap94 127 28 a a DT cord-014901-d9szap94 127 29 special special JJ cord-014901-d9szap94 127 30 interest interest NN cord-014901-d9szap94 127 31 . . . cord-014901-d9szap94 128 1 For for IN cord-014901-d9szap94 128 2 example example NN cord-014901-d9szap94 128 3 , , , cord-014901-d9szap94 128 4 the the DT cord-014901-d9szap94 128 5 usage usage NN cord-014901-d9szap94 128 6 of of IN cord-014901-d9szap94 128 7 promoters promoter NNS cord-014901-d9szap94 128 8 directing direct VBG cord-014901-d9szap94 128 9 transcription transcription NN cord-014901-d9szap94 128 10 of of IN cord-014901-d9szap94 128 11 the the DT cord-014901-d9szap94 128 12 target target NN cord-014901-d9szap94 128 13 gene gene NN cord-014901-d9szap94 128 14 predominantly predominantly RB cord-014901-d9szap94 128 15 in in IN cord-014901-d9szap94 128 16 the the DT cord-014901-d9szap94 128 17 seeds seed NNS cord-014901-d9szap94 128 18 can can MD cord-014901-d9szap94 128 19 increase increase VB cord-014901-d9szap94 128 20 the the DT cord-014901-d9szap94 128 21 yield yield NN cord-014901-d9szap94 128 22 of of IN cord-014901-d9szap94 128 23 the the DT cord-014901-d9szap94 128 24 target target NN cord-014901-d9szap94 128 25 protein protein NN cord-014901-d9szap94 128 26 by by IN cord-014901-d9szap94 128 27 an an DT cord-014901-d9szap94 128 28 order order NN cord-014901-d9szap94 128 29 or or CC cord-014901-d9szap94 128 30 several several JJ cord-014901-d9szap94 128 31 fold fold NN cord-014901-d9szap94 128 32 : : : cord-014901-d9szap94 128 33 up up IN cord-014901-d9szap94 128 34 to to TO cord-014901-d9szap94 128 35 13 13 CD cord-014901-d9szap94 128 36 - - SYM cord-014901-d9szap94 128 37 14 14 CD cord-014901-d9szap94 128 38 % % NN cord-014901-d9szap94 128 39 of of IN cord-014901-d9szap94 128 40 TSP tsp NN cord-014901-d9szap94 128 41 in in IN cord-014901-d9szap94 128 42 rice rice NN cord-014901-d9szap94 128 43 grains grain NNS cord-014901-d9szap94 128 44 and and CC cord-014901-d9szap94 128 45 up up IN cord-014901-d9szap94 128 46 to to TO cord-014901-d9szap94 128 47 25 25 CD cord-014901-d9szap94 128 48 % % NN cord-014901-d9szap94 128 49 of of IN cord-014901-d9szap94 128 50 TSP tsp NN cord-014901-d9szap94 128 51 in in IN cord-014901-d9szap94 128 52 tobacco tobacco NN cord-014901-d9szap94 128 53 seeds seed NNS cord-014901-d9szap94 128 54 [ [ -LRB- cord-014901-d9szap94 128 55 17 17 CD cord-014901-d9szap94 128 56 ] ] -RRB- cord-014901-d9szap94 128 57 . . . cord-014901-d9szap94 129 1 It -PRON- PRP cord-014901-d9szap94 129 2 is be VBZ cord-014901-d9szap94 129 3 of of IN cord-014901-d9szap94 129 4 importance importance NN cord-014901-d9szap94 129 5 that that WDT cord-014901-d9szap94 129 6 as as IN cord-014901-d9szap94 129 7 compared compare VBN cord-014901-d9szap94 129 8 with with IN cord-014901-d9szap94 129 9 leaves leave NNS cord-014901-d9szap94 129 10 , , , cord-014901-d9szap94 129 11 seeds seed NNS cord-014901-d9szap94 129 12 contain contain VBP cord-014901-d9szap94 129 13 less less RBR cord-014901-d9szap94 129 14 pro pro JJ cord-014901-d9szap94 129 15 teases tease NNS cord-014901-d9szap94 129 16 and and CC cord-014901-d9szap94 129 17 much much RB cord-014901-d9szap94 129 18 less less JJR cord-014901-d9szap94 129 19 water water NN cord-014901-d9szap94 129 20 ; ; : cord-014901-d9szap94 129 21 therefore therefore RB cord-014901-d9szap94 129 22 , , , cord-014901-d9szap94 129 23 recombinant recombinant JJ cord-014901-d9szap94 129 24 protein protein NN cord-014901-d9szap94 129 25 is be VBZ cord-014901-d9szap94 129 26 saved save VBN cord-014901-d9szap94 129 27 better well RBR cord-014901-d9szap94 129 28 . . . cord-014901-d9szap94 130 1 Despite despite IN cord-014901-d9szap94 130 2 the the DT cord-014901-d9szap94 130 3 fact fact NN cord-014901-d9szap94 130 4 that that IN cord-014901-d9szap94 130 5 transgenic transgenic JJ cord-014901-d9szap94 130 6 plants plant NNS cord-014901-d9szap94 130 7 with with IN cord-014901-d9szap94 130 8 stable stable JJ cord-014901-d9szap94 130 9 transgene transgene NN cord-014901-d9szap94 130 10 expression expression NN cord-014901-d9szap94 130 11 in in IN cord-014901-d9szap94 130 12 the the DT cord-014901-d9szap94 130 13 nuclear nuclear JJ cord-014901-d9szap94 130 14 genome genome NN cord-014901-d9szap94 130 15 are be VBP cord-014901-d9szap94 130 16 used use VBN cord-014901-d9szap94 130 17 most most RBS cord-014901-d9szap94 130 18 widely widely RB cord-014901-d9szap94 130 19 , , , cord-014901-d9szap94 130 20 it -PRON- PRP cord-014901-d9szap94 130 21 is be VBZ cord-014901-d9szap94 130 22 just just RB cord-014901-d9szap94 130 23 this this DT cord-014901-d9szap94 130 24 expression expression NN cord-014901-d9szap94 130 25 system system NN cord-014901-d9szap94 130 26 that that WDT cord-014901-d9szap94 130 27 induces induce VBZ cord-014901-d9szap94 130 28 a a DT cord-014901-d9szap94 130 29 cautious cautious JJ cord-014901-d9szap94 130 30 attitude attitude NN cord-014901-d9szap94 130 31 of of IN cord-014901-d9szap94 130 32 human human JJ cord-014901-d9szap94 130 33 society society NN cord-014901-d9szap94 130 34 . . . cord-014901-d9szap94 131 1 One one CD cord-014901-d9szap94 131 2 of of IN cord-014901-d9szap94 131 3 the the DT cord-014901-d9szap94 131 4 fears fear NNS cord-014901-d9szap94 131 5 is be VBZ cord-014901-d9szap94 131 6 associated associate VBN cord-014901-d9szap94 131 7 with with IN cord-014901-d9szap94 131 8 the the DT cord-014901-d9szap94 131 9 possibility possibility NN cord-014901-d9szap94 131 10 of of IN cord-014901-d9szap94 131 11 transgene transgene NN cord-014901-d9szap94 131 12 transfer transfer NN cord-014901-d9szap94 131 13 from from IN cord-014901-d9szap94 131 14 the the DT cord-014901-d9szap94 131 15 cross cross NN cord-014901-d9szap94 131 16 pollinated pollinate VBD cord-014901-d9szap94 131 17 plants plant NNS cord-014901-d9szap94 131 18 into into IN cord-014901-d9szap94 131 19 the the DT cord-014901-d9szap94 131 20 genomes genome NNS cord-014901-d9szap94 131 21 of of IN cord-014901-d9szap94 131 22 wild wild JJ cord-014901-d9szap94 131 23 relatives relative NNS cord-014901-d9szap94 131 24 at at IN cord-014901-d9szap94 131 25 growing grow VBG cord-014901-d9szap94 131 26 biotechnological biotechnological JJ cord-014901-d9szap94 131 27 crops crop NNS cord-014901-d9szap94 131 28 in in IN cord-014901-d9szap94 131 29 open open JJ cord-014901-d9szap94 131 30 field field NN cord-014901-d9szap94 131 31 . . . cord-014901-d9szap94 132 1 To to TO cord-014901-d9szap94 132 2 solve solve VB cord-014901-d9szap94 132 3 this this DT cord-014901-d9szap94 132 4 problem problem NN cord-014901-d9szap94 132 5 , , , cord-014901-d9szap94 132 6 researchers researcher NNS cord-014901-d9szap94 132 7 developed develop VBD cord-014901-d9szap94 132 8 various various JJ cord-014901-d9szap94 132 9 agricultural agricultural JJ cord-014901-d9szap94 132 10 technologies technology NNS cord-014901-d9szap94 132 11 as as RB cord-014901-d9szap94 132 12 well well RB cord-014901-d9szap94 132 13 as as IN cord-014901-d9szap94 132 14 fixing fix VBG cord-014901-d9szap94 132 15 male male JJ cord-014901-d9szap94 132 16 sterility sterility NN cord-014901-d9szap94 132 17 in in IN cord-014901-d9szap94 132 18 transgenic transgenic JJ cord-014901-d9szap94 132 19 plants plant NNS cord-014901-d9szap94 132 20 to to TO cord-014901-d9szap94 132 21 prevent prevent VB cord-014901-d9szap94 132 22 unwanted unwanted JJ cord-014901-d9szap94 132 23 cross cross NN cord-014901-d9szap94 132 24 pollination pollination NN cord-014901-d9szap94 132 25 of of IN cord-014901-d9szap94 132 26 cultivated cultivated JJ cord-014901-d9szap94 132 27 and and CC cord-014901-d9szap94 132 28 wild wild JJ cord-014901-d9szap94 132 29 spe spe NNP cord-014901-d9szap94 132 30 cies cie NNS cord-014901-d9szap94 132 31 . . . cord-014901-d9szap94 133 1 Examples example NNS cord-014901-d9szap94 133 2 of of IN cord-014901-d9szap94 133 3 production production NN cord-014901-d9szap94 133 4 of of IN cord-014901-d9szap94 133 5 various various JJ cord-014901-d9szap94 133 6 immunogenic immunogenic JJ cord-014901-d9szap94 133 7 proteins protein NNS cord-014901-d9szap94 133 8 using use VBG cord-014901-d9szap94 133 9 for for IN cord-014901-d9szap94 133 10 this this DT cord-014901-d9szap94 133 11 purpose purpose NN cord-014901-d9szap94 133 12 transgenic transgenic JJ cord-014901-d9szap94 133 13 plants plant NNS cord-014901-d9szap94 133 14 with with IN cord-014901-d9szap94 133 15 stable stable JJ cord-014901-d9szap94 133 16 transgene transgene NN cord-014901-d9szap94 133 17 integration integration NN cord-014901-d9szap94 133 18 into into IN cord-014901-d9szap94 133 19 the the DT cord-014901-d9szap94 133 20 nuclear nuclear JJ cord-014901-d9szap94 133 21 genome genome NN cord-014901-d9szap94 133 22 are be VBP cord-014901-d9szap94 133 23 presented present VBN cord-014901-d9szap94 133 24 in in IN cord-014901-d9szap94 133 25 Table Table NNP cord-014901-d9szap94 133 26 1 1 CD cord-014901-d9szap94 133 27 . . . cord-014901-d9szap94 134 1 Chloroplasts chloroplast NNS cord-014901-d9szap94 134 2 are be VBP cord-014901-d9szap94 134 3 most most RBS cord-014901-d9szap94 134 4 attractive attractive JJ cord-014901-d9szap94 134 5 among among IN cord-014901-d9szap94 134 6 plant plant NN cord-014901-d9szap94 134 7 expression expression NN cord-014901-d9szap94 134 8 systems system NNS cord-014901-d9szap94 134 9 . . . cord-014901-d9szap94 135 1 Genetically genetically RB cord-014901-d9szap94 135 2 modified modify VBN cord-014901-d9szap94 135 3 plants plant NNS cord-014901-d9szap94 135 4 with with IN cord-014901-d9szap94 135 5 the the DT cord-014901-d9szap94 135 6 stable stable JJ cord-014901-d9szap94 135 7 transgene transgene NN cord-014901-d9szap94 135 8 integration integration NN cord-014901-d9szap94 135 9 into into IN cord-014901-d9szap94 135 10 the the DT cord-014901-d9szap94 135 11 chloroplast chloroplast NN cord-014901-d9szap94 135 12 genome genome NN cord-014901-d9szap94 135 13 were be VBD cord-014901-d9szap94 135 14 called call VBN cord-014901-d9szap94 135 15 transplastome transplastome VBN cord-014901-d9szap94 135 16 plants plant NNS cord-014901-d9szap94 135 17 . . . cord-014901-d9szap94 136 1 The the DT cord-014901-d9szap94 136 2 specific specific JJ cord-014901-d9szap94 136 3 organization organization NN cord-014901-d9szap94 136 4 of of IN cord-014901-d9szap94 136 5 chloroplasts chloroplast NNS cord-014901-d9szap94 136 6 allows allow VBZ cord-014901-d9szap94 136 7 achieving achieve VBG cord-014901-d9szap94 136 8 a a DT cord-014901-d9szap94 136 9 high high JJ cord-014901-d9szap94 136 10 dose dose NN cord-014901-d9szap94 136 11 of of IN cord-014901-d9szap94 136 12 foreign foreign JJ cord-014901-d9szap94 136 13 gene gene NN cord-014901-d9szap94 136 14 in in IN cord-014901-d9szap94 136 15 transplastome transplastome NNP cord-014901-d9szap94 136 16 plants plant NNS cord-014901-d9szap94 136 17 , , , cord-014901-d9szap94 136 18 which which WDT cord-014901-d9szap94 136 19 provides provide VBZ cord-014901-d9szap94 136 20 for for IN cord-014901-d9szap94 136 21 the the DT cord-014901-d9szap94 136 22 efficient efficient JJ cord-014901-d9szap94 136 23 production production NN cord-014901-d9szap94 136 24 of of IN cord-014901-d9szap94 136 25 the the DT cord-014901-d9szap94 136 26 target target NN cord-014901-d9szap94 136 27 pro pro FW cord-014901-d9szap94 136 28 tein tein NNP cord-014901-d9szap94 136 29 . . . cord-014901-d9szap94 137 1 The the DT cord-014901-d9szap94 137 2 record record NN cord-014901-d9szap94 137 3 of of IN cord-014901-d9szap94 137 4 the the DT cord-014901-d9szap94 137 5 recombinant recombinant JJ cord-014901-d9szap94 137 6 protein protein NN cord-014901-d9szap94 137 7 yield yield NN cord-014901-d9szap94 137 8 was be VBD cord-014901-d9szap94 137 9 achieved achieve VBN cord-014901-d9szap94 137 10 by by IN cord-014901-d9szap94 137 11 transplastomic transplastomic JJ cord-014901-d9szap94 137 12 tobacco tobacco NN cord-014901-d9szap94 137 13 plants plant NNS cord-014901-d9szap94 137 14 with with IN cord-014901-d9szap94 137 15 the the DT cord-014901-d9szap94 137 16 bacteriophage bacteriophage NN cord-014901-d9szap94 137 17 lysin lysin NNP cord-014901-d9szap94 137 18 gene gene NNP cord-014901-d9szap94 137 19 PlyGBS PlyGBS NNP cord-014901-d9szap94 137 20 , , , cord-014901-d9szap94 137 21 encoding encode VBG cord-014901-d9szap94 137 22 a a DT cord-014901-d9szap94 137 23 hydro hydro NN cord-014901-d9szap94 137 24 lase lase NN cord-014901-d9szap94 137 25 of of IN cord-014901-d9szap94 137 26 the the DT cord-014901-d9szap94 137 27 bacterial bacterial JJ cord-014901-d9szap94 137 28 cell cell NN cord-014901-d9szap94 137 29 wall wall NN cord-014901-d9szap94 137 30 , , , cord-014901-d9szap94 137 31 the the DT cord-014901-d9szap94 137 32 level level NN cord-014901-d9szap94 137 33 of of IN cord-014901-d9szap94 137 34 which which WDT cord-014901-d9szap94 137 35 accu accu NNP cord-014901-d9szap94 137 36 mulation mulation NN cord-014901-d9szap94 137 37 in in IN cord-014901-d9szap94 137 38 the the DT cord-014901-d9szap94 137 39 leaves leave NNS cord-014901-d9szap94 137 40 amounted amount VBD cord-014901-d9szap94 137 41 to to IN cord-014901-d9szap94 137 42 70 70 CD cord-014901-d9szap94 137 43 % % NN cord-014901-d9szap94 137 44 of of IN cord-014901-d9szap94 137 45 TSP tsp NN cord-014901-d9szap94 137 46 [ [ -LRB- cord-014901-d9szap94 137 47 32 32 CD cord-014901-d9szap94 137 48 ] ] -RRB- cord-014901-d9szap94 137 49 . . . cord-014901-d9szap94 138 1 Although although IN cord-014901-d9szap94 138 2 in in IN cord-014901-d9szap94 138 3 some some DT cord-014901-d9szap94 138 4 cases case NNS cord-014901-d9szap94 138 5 , , , cord-014901-d9szap94 138 6 negative negative JJ cord-014901-d9szap94 138 7 physiological physiological JJ cord-014901-d9szap94 138 8 changes change NNS cord-014901-d9szap94 138 9 were be VBD cord-014901-d9szap94 138 10 observed observe VBN cord-014901-d9szap94 138 11 in in IN cord-014901-d9szap94 138 12 plants plant NNS cord-014901-d9szap94 138 13 with with IN cord-014901-d9szap94 138 14 such such JJ cord-014901-d9szap94 138 15 high high JJ cord-014901-d9szap94 138 16 level level NN cord-014901-d9szap94 138 17 of of IN cord-014901-d9szap94 138 18 foreign foreign JJ cord-014901-d9szap94 138 19 gene gene NN cord-014901-d9szap94 138 20 expression expression NN cord-014901-d9szap94 138 21 , , , cord-014901-d9szap94 138 22 usually usually RB cord-014901-d9szap94 138 23 there there EX cord-014901-d9szap94 138 24 were be VBD cord-014901-d9szap94 138 25 no no DT cord-014901-d9szap94 138 26 devia devia FW cord-014901-d9szap94 138 27 tions tion NNS cord-014901-d9szap94 138 28 in in IN cord-014901-d9szap94 138 29 the the DT cord-014901-d9szap94 138 30 development development NN cord-014901-d9szap94 138 31 of of IN cord-014901-d9szap94 138 32 such such JJ cord-014901-d9szap94 138 33 plants plant NNS cord-014901-d9szap94 138 34 . . . cord-014901-d9szap94 139 1 Problems problem NNS cord-014901-d9szap94 139 2 of of IN cord-014901-d9szap94 139 3 transplastome transplastome JJ cord-014901-d9szap94 139 4 plant plant NN cord-014901-d9szap94 139 5 adaptation adaptation NN cord-014901-d9szap94 139 6 to to IN cord-014901-d9szap94 139 7 the the DT cord-014901-d9szap94 139 8 high high JJ cord-014901-d9szap94 139 9 level level NN cord-014901-d9szap94 139 10 of of IN cord-014901-d9szap94 139 11 for for IN cord-014901-d9szap94 139 12 eign eign NNP cord-014901-d9szap94 139 13 gene gene NN cord-014901-d9szap94 139 14 expression expression NN cord-014901-d9szap94 139 15 are be VBP cord-014901-d9szap94 139 16 discussed discuss VBN cord-014901-d9szap94 139 17 in in IN cord-014901-d9szap94 139 18 the the DT cord-014901-d9szap94 139 19 review review NN cord-014901-d9szap94 139 20 of of IN cord-014901-d9szap94 139 21 Bally Bally NNP cord-014901-d9szap94 139 22 et et NNP cord-014901-d9szap94 139 23 al al NNP cord-014901-d9szap94 139 24 . . . cord-014901-d9szap94 140 1 [ [ -LRB- cord-014901-d9szap94 140 2 33 33 CD cord-014901-d9szap94 140 3 ] ] -RRB- cord-014901-d9szap94 140 4 . . . cord-014901-d9szap94 141 1 Transgene transgene NN cord-014901-d9szap94 141 2 delivery delivery NN cord-014901-d9szap94 141 3 to to IN cord-014901-d9szap94 141 4 chloroplasts chloroplast NNS cord-014901-d9szap94 141 5 is be VBZ cord-014901-d9szap94 141 6 performed perform VBN cord-014901-d9szap94 141 7 using use VBG cord-014901-d9szap94 141 8 bioballistics bioballistic NNS cord-014901-d9szap94 141 9 ( ( -LRB- cord-014901-d9szap94 141 10 gene gene NNP cord-014901-d9szap94 141 11 gun gun NN cord-014901-d9szap94 141 12 ) ) -RRB- cord-014901-d9szap94 141 13 ; ; : cord-014901-d9szap94 141 14 its -PRON- PRP$ cord-014901-d9szap94 141 15 integration integration NN cord-014901-d9szap94 141 16 into into IN cord-014901-d9szap94 141 17 the the DT cord-014901-d9szap94 141 18 chloroplast chloroplast NN cord-014901-d9szap94 141 19 genome genome NN cord-014901-d9szap94 141 20 occurs occur VBZ cord-014901-d9szap94 141 21 via via IN cord-014901-d9szap94 141 22 homological homological JJ cord-014901-d9szap94 141 23 recombi recombi NN cord-014901-d9szap94 141 24 nation nation NN cord-014901-d9szap94 141 25 [ [ -LRB- cord-014901-d9szap94 141 26 34 34 CD cord-014901-d9szap94 141 27 ] ] -RRB- cord-014901-d9szap94 141 28 . . . cord-014901-d9szap94 142 1 The the DT cord-014901-d9szap94 142 2 advantage advantage NN cord-014901-d9szap94 142 3 of of IN cord-014901-d9szap94 142 4 chloroplast chloroplast JJ cord-014901-d9szap94 142 5 expression expression NN cord-014901-d9szap94 142 6 system system NN cord-014901-d9szap94 142 7 is be VBZ cord-014901-d9szap94 142 8 in in IN cord-014901-d9szap94 142 9 the the DT cord-014901-d9szap94 142 10 absence absence NN cord-014901-d9szap94 142 11 of of IN cord-014901-d9szap94 142 12 the the DT cord-014901-d9szap94 142 13 effect effect NN cord-014901-d9szap94 142 14 of of IN cord-014901-d9szap94 142 15 position position NN cord-014901-d9szap94 142 16 observed observe VBN cord-014901-d9szap94 142 17 in in IN cord-014901-d9szap94 142 18 the the DT cord-014901-d9szap94 142 19 case case NN cord-014901-d9szap94 142 20 of of IN cord-014901-d9szap94 142 21 random random JJ cord-014901-d9szap94 142 22 pattern pattern NN cord-014901-d9szap94 142 23 of of IN cord-014901-d9szap94 142 24 transgene transgene NN cord-014901-d9szap94 142 25 distribution distribution NN cord-014901-d9szap94 142 26 in in IN cord-014901-d9szap94 142 27 the the DT cord-014901-d9szap94 142 28 nuclear nuclear JJ cord-014901-d9szap94 142 29 genome genome NN cord-014901-d9szap94 142 30 . . . cord-014901-d9szap94 143 1 In in IN cord-014901-d9szap94 143 2 plastids plastid NNS cord-014901-d9szap94 143 3 , , , cord-014901-d9szap94 143 4 there there EX cord-014901-d9szap94 143 5 is be VBZ cord-014901-d9szap94 143 6 no no DT cord-014901-d9szap94 143 7 transgene transgene NN cord-014901-d9szap94 143 8 splicing splicing NN cord-014901-d9szap94 143 9 ( ( -LRB- cord-014901-d9szap94 143 10 inactivation inactivation NN cord-014901-d9szap94 143 11 ) ) -RRB- cord-014901-d9szap94 143 12 ; ; : cord-014901-d9szap94 143 13 therefore therefore RB cord-014901-d9szap94 143 14 , , , cord-014901-d9szap94 143 15 its -PRON- PRP$ cord-014901-d9szap94 143 16 expression expression NN cord-014901-d9szap94 143 17 is be VBZ cord-014901-d9szap94 143 18 stably stably RB cord-014901-d9szap94 143 19 preserved preserve VBN cord-014901-d9szap94 143 20 in in IN cord-014901-d9szap94 143 21 subsequent subsequent JJ cord-014901-d9szap94 143 22 genera genera NN cord-014901-d9szap94 143 23 tions tion NNS cord-014901-d9szap94 143 24 . . . cord-014901-d9szap94 144 1 Due due IN cord-014901-d9szap94 144 2 to to IN cord-014901-d9szap94 144 3 the the DT cord-014901-d9szap94 144 4 prokaryotic prokaryotic JJ cord-014901-d9szap94 144 5 organization organization NN cord-014901-d9szap94 144 6 of of IN cord-014901-d9szap94 144 7 chloro chloro NNP cord-014901-d9szap94 144 8 plast plast NNP cord-014901-d9szap94 144 9 genome genome NNP cord-014901-d9szap94 144 10 , , , cord-014901-d9szap94 144 11 there there EX cord-014901-d9szap94 144 12 is be VBZ cord-014901-d9szap94 144 13 a a DT cord-014901-d9szap94 144 14 possibility possibility NN cord-014901-d9szap94 144 15 of of IN cord-014901-d9szap94 144 16 co co JJ cord-014901-d9szap94 144 17 expression expression NN cord-014901-d9szap94 144 18 of of IN cord-014901-d9szap94 144 19 several several JJ cord-014901-d9szap94 144 20 genes gene NNS cord-014901-d9szap94 144 21 within within IN cord-014901-d9szap94 144 22 a a DT cord-014901-d9szap94 144 23 single single JJ cord-014901-d9szap94 144 24 operon operon NN cord-014901-d9szap94 144 25 [ [ -LRB- cord-014901-d9szap94 144 26 35 35 CD cord-014901-d9szap94 144 27 ] ] -RRB- cord-014901-d9szap94 144 28 . . . cord-014901-d9szap94 145 1 An an DT cord-014901-d9szap94 145 2 impor impor JJ cord-014901-d9szap94 145 3 tant tant FW cord-014901-d9szap94 145 4 specificity specificity NN cord-014901-d9szap94 145 5 of of IN cord-014901-d9szap94 145 6 plastids plastid NNS cord-014901-d9szap94 145 7 is be VBZ cord-014901-d9szap94 145 8 that that IN cord-014901-d9szap94 145 9 they -PRON- PRP cord-014901-d9szap94 145 10 are be VBP cord-014901-d9szap94 145 11 inherited inherit VBN cord-014901-d9szap94 145 12 through through IN cord-014901-d9szap94 145 13 the the DT cord-014901-d9szap94 145 14 maternal maternal JJ cord-014901-d9szap94 145 15 line line NN cord-014901-d9szap94 145 16 and and CC cord-014901-d9szap94 145 17 usually usually RB cord-014901-d9szap94 145 18 are be VBP cord-014901-d9szap94 145 19 absent absent JJ cord-014901-d9szap94 145 20 from from IN cord-014901-d9szap94 145 21 pollen pollen NN cord-014901-d9szap94 145 22 . . . cord-014901-d9szap94 146 1 Therefore therefore RB cord-014901-d9szap94 146 2 , , , cord-014901-d9szap94 146 3 as as IN cord-014901-d9szap94 146 4 distinct distinct JJ cord-014901-d9szap94 146 5 from from IN cord-014901-d9szap94 146 6 usual usual JJ cord-014901-d9szap94 146 7 transgenic transgenic JJ cord-014901-d9szap94 146 8 plants plant NNS cord-014901-d9szap94 146 9 , , , cord-014901-d9szap94 146 10 transplastome transplastome JJ cord-014901-d9szap94 146 11 plants plant NNS cord-014901-d9szap94 146 12 are be VBP cord-014901-d9szap94 146 13 safe safe JJ cord-014901-d9szap94 146 14 for for IN cord-014901-d9szap94 146 15 environment environment NN cord-014901-d9szap94 146 16 because because IN cord-014901-d9szap94 146 17 the the DT cord-014901-d9szap94 146 18 uncontrolled uncontrolled JJ cord-014901-d9szap94 146 19 spread spread NN cord-014901-d9szap94 146 20 of of IN cord-014901-d9szap94 146 21 the the DT cord-014901-d9szap94 146 22 transgene transgene NN cord-014901-d9szap94 146 23 into into IN cord-014901-d9szap94 146 24 other other JJ cord-014901-d9szap94 146 25 plants plant NNS cord-014901-d9szap94 146 26 is be VBZ cord-014901-d9szap94 146 27 prevented prevent VBN cord-014901-d9szap94 146 28 [ [ -LRB- cord-014901-d9szap94 146 29 36 36 CD cord-014901-d9szap94 146 30 , , , cord-014901-d9szap94 146 31 37 37 CD cord-014901-d9szap94 146 32 ] ] -RRB- cord-014901-d9szap94 146 33 . . . cord-014901-d9szap94 147 1 It -PRON- PRP cord-014901-d9szap94 147 2 should should MD cord-014901-d9szap94 147 3 be be VB cord-014901-d9szap94 147 4 noted note VBN cord-014901-d9szap94 147 5 that that IN cord-014901-d9szap94 147 6 protein protein NN cord-014901-d9szap94 147 7 posttranslational posttranslational JJ cord-014901-d9szap94 147 8 modifications modification NNS cord-014901-d9szap94 147 9 , , , cord-014901-d9szap94 147 10 e.g. e.g. RB cord-014901-d9szap94 147 11 , , , cord-014901-d9szap94 147 12 assembling assemble VBG cord-014901-d9szap94 147 13 multimer multimer NN cord-014901-d9szap94 147 14 proteins protein NNS cord-014901-d9szap94 147 15 , , , cord-014901-d9szap94 147 16 the the DT cord-014901-d9szap94 147 17 TMV TMV NNP cord-014901-d9szap94 147 18 - - HYPH cord-014901-d9szap94 147 19 tobacco tobacco NN cord-014901-d9szap94 147 20 mosaic mosaic NN cord-014901-d9szap94 147 21 virus virus NN cord-014901-d9szap94 147 22 ; ; : cord-014901-d9szap94 147 23 CaMV CaMV NNP cord-014901-d9szap94 147 24 - - HYPH cord-014901-d9szap94 147 25 cauliflower cauliflower NN cord-014901-d9szap94 147 26 mosaic mosaic JJ cord-014901-d9szap94 147 27 virus virus NN cord-014901-d9szap94 147 28 ; ; : cord-014901-d9szap94 147 29 TSP tsp NN cord-014901-d9szap94 147 30 - - HYPH cord-014901-d9szap94 147 31 total total JJ cord-014901-d9szap94 147 32 soluble soluble JJ cord-014901-d9szap94 147 33 protein protein NN cord-014901-d9szap94 147 34 ; ; : cord-014901-d9szap94 147 35 ( ( -LRB- cord-014901-d9szap94 147 36 + + NFP cord-014901-d9szap94 147 37 ) ) -RRB- cord-014901-d9szap94 147 38 immune immune JJ cord-014901-d9szap94 147 39 response response NN cord-014901-d9szap94 147 40 is be VBZ cord-014901-d9szap94 147 41 revealed reveal VBN cord-014901-d9szap94 147 42 at at IN cord-014901-d9szap94 147 43 oral oral JJ cord-014901-d9szap94 147 44 immunization immunization NN cord-014901-d9szap94 147 45 ; ; : cord-014901-d9szap94 147 46 ( ( -LRB- cord-014901-d9szap94 147 47 + + NNS cord-014901-d9szap94 147 48 + + SYM cord-014901-d9szap94 147 49 ) ) -RRB- cord-014901-d9szap94 147 50 immune immune JJ cord-014901-d9szap94 147 51 response response NN cord-014901-d9szap94 147 52 is be VBZ cord-014901-d9szap94 147 53 revealed reveal VBN cord-014901-d9szap94 147 54 at at IN cord-014901-d9szap94 147 55 oral oral JJ cord-014901-d9szap94 147 56 immunization immunization NN cord-014901-d9szap94 147 57 , , , cord-014901-d9szap94 147 58 animals animal NNS cord-014901-d9szap94 147 59 did do VBD cord-014901-d9szap94 147 60 not not RB cord-014901-d9szap94 147 61 die die VB cord-014901-d9szap94 147 62 after after IN cord-014901-d9szap94 147 63 virus virus NN cord-014901-d9szap94 147 64 infection infection NN cord-014901-d9szap94 147 65 . . . cord-014901-d9szap94 148 1 Species specie NNS cord-014901-d9szap94 148 2 of of IN cord-014901-d9szap94 148 3 immunized immunize VBN cord-014901-d9szap94 148 4 animals animal NNS cord-014901-d9szap94 148 5 are be VBP cord-014901-d9szap94 148 6 indicated indicate VBN cord-014901-d9szap94 148 7 , , , cord-014901-d9szap94 148 8 the the DT cord-014901-d9szap94 148 9 way way NN cord-014901-d9szap94 148 10 of of IN cord-014901-d9szap94 148 11 antigen antigen NN cord-014901-d9szap94 148 12 delivery delivery NN cord-014901-d9szap94 148 13 is be VBZ cord-014901-d9szap94 148 14 indicated indicate VBN cord-014901-d9szap94 148 15 in in IN cord-014901-d9szap94 148 16 the the DT cord-014901-d9szap94 148 17 cases case NNS cord-014901-d9szap94 148 18 when when WRB cord-014901-d9szap94 148 19 it -PRON- PRP cord-014901-d9szap94 148 20 was be VBD cord-014901-d9szap94 148 21 not not RB cord-014901-d9szap94 148 22 oral oral JJ cord-014901-d9szap94 148 23 ; ; : cord-014901-d9szap94 148 24 the the DT cord-014901-d9szap94 148 25 amount amount NN cord-014901-d9szap94 148 26 of of IN cord-014901-d9szap94 148 27 survived survive VBD cord-014901-d9szap94 148 28 infected infected JJ cord-014901-d9szap94 148 29 animals animal NNS cord-014901-d9szap94 148 30 is be VBZ cord-014901-d9szap94 148 31 indicated indicate VBN cord-014901-d9szap94 148 32 in in IN cord-014901-d9szap94 148 33 the the DT cord-014901-d9szap94 148 34 cases case NNS cord-014901-d9szap94 148 35 when when WRB cord-014901-d9szap94 148 36 the the DT cord-014901-d9szap94 148 37 survival survival NN cord-014901-d9szap94 148 38 was be VBD cord-014901-d9szap94 148 39 less less JJR cord-014901-d9szap94 148 40 than than IN cord-014901-d9szap94 148 41 100 100 CD cord-014901-d9szap94 148 42 % % NN cord-014901-d9szap94 148 43 . . . cord-014901-d9szap94 149 1 No no UH cord-014901-d9szap94 149 2 . . NN cord-014901-d9szap94 149 3 1 1 CD cord-014901-d9szap94 149 4 2015 2015 CD cord-014901-d9szap94 149 5 formation formation NN cord-014901-d9szap94 149 6 of of IN cord-014901-d9szap94 149 7 disulfide disulfide NN cord-014901-d9szap94 149 8 bridges bridge NNS cord-014901-d9szap94 149 9 , , , cord-014901-d9szap94 149 10 lipid lipid NN cord-014901-d9szap94 149 11 modifications modification NNS cord-014901-d9szap94 149 12 , , , cord-014901-d9szap94 149 13 etc etc FW cord-014901-d9szap94 149 14 . . FW cord-014901-d9szap94 149 15 , , , cord-014901-d9szap94 149 16 occur occur VBP cord-014901-d9szap94 149 17 successfully successfully RB cord-014901-d9szap94 149 18 in in IN cord-014901-d9szap94 149 19 chloroplasts chloroplast NNS cord-014901-d9szap94 149 20 . . . cord-014901-d9szap94 150 1 It -PRON- PRP cord-014901-d9szap94 150 2 is be VBZ cord-014901-d9szap94 150 3 estab estab NNP cord-014901-d9szap94 150 4 lished lishe VBN cord-014901-d9szap94 150 5 that that IN cord-014901-d9szap94 150 6 recombinant recombinant JJ cord-014901-d9szap94 150 7 proteins protein NNS cord-014901-d9szap94 150 8 synthesized synthesize VBN cord-014901-d9szap94 150 9 in in IN cord-014901-d9szap94 150 10 chlo chlo NN cord-014901-d9szap94 150 11 roplasts roplast NNS cord-014901-d9szap94 150 12 do do VBP cord-014901-d9szap94 150 13 not not RB cord-014901-d9szap94 150 14 differ differ VB cord-014901-d9szap94 150 15 from from IN cord-014901-d9szap94 150 16 native native JJ cord-014901-d9szap94 150 17 ones one NNS cord-014901-d9szap94 150 18 in in IN cord-014901-d9szap94 150 19 their -PRON- PRP$ cord-014901-d9szap94 150 20 func func NN cord-014901-d9szap94 150 21 tional tional JJ cord-014901-d9szap94 150 22 activities activity NNS cord-014901-d9szap94 150 23 [ [ -LRB- cord-014901-d9szap94 150 24 38 38 CD cord-014901-d9szap94 150 25 , , , cord-014901-d9szap94 150 26 39 39 CD cord-014901-d9szap94 150 27 ] ] -RRB- cord-014901-d9szap94 150 28 . . . cord-014901-d9szap94 151 1 Chloroplast chloroplast JJ cord-014901-d9szap94 151 2 attractiveness attractiveness NN cord-014901-d9szap94 151 3 as as IN cord-014901-d9szap94 151 4 the the DT cord-014901-d9szap94 151 5 system system NN cord-014901-d9szap94 151 6 of of IN cord-014901-d9szap94 151 7 recombinant recombinant JJ cord-014901-d9szap94 151 8 protein protein NN cord-014901-d9szap94 151 9 expression expression NN cord-014901-d9szap94 151 10 is be VBZ cord-014901-d9szap94 151 11 in in IN cord-014901-d9szap94 151 12 the the DT cord-014901-d9szap94 151 13 fact fact NN cord-014901-d9szap94 151 14 that that IN cord-014901-d9szap94 151 15 they -PRON- PRP cord-014901-d9szap94 151 16 are be VBP cord-014901-d9szap94 151 17 closed closed JJ cord-014901-d9szap94 151 18 structures structure NNS cord-014901-d9szap94 151 19 and and CC cord-014901-d9szap94 151 20 it -PRON- PRP cord-014901-d9szap94 151 21 preserves preserve VBZ cord-014901-d9szap94 151 22 the the DT cord-014901-d9szap94 151 23 metabolites metabolite NNS cord-014901-d9szap94 151 24 , , , cord-014901-d9szap94 151 25 which which WDT cord-014901-d9szap94 151 26 when when WRB cord-014901-d9szap94 151 27 released release VBN cord-014901-d9szap94 151 28 into into IN cord-014901-d9szap94 151 29 the the DT cord-014901-d9szap94 151 30 cytosol cytosol NN cord-014901-d9szap94 151 31 are be VBP cord-014901-d9szap94 151 32 toxic toxic JJ cord-014901-d9szap94 151 33 to to TO cord-014901-d9szap94 151 34 plant plant NN cord-014901-d9szap94 151 35 cells cell NNS cord-014901-d9szap94 151 36 , , , cord-014901-d9szap94 151 37 such such JJ cord-014901-d9szap94 151 38 as as IN cord-014901-d9szap94 151 39 B B NNP cord-014901-d9szap94 151 40 subunit subunit NN cord-014901-d9szap94 151 41 of of IN cord-014901-d9szap94 151 42 cholera cholera NN cord-014901-d9szap94 151 43 toxin toxin NN cord-014901-d9szap94 151 44 [ [ -LRB- cord-014901-d9szap94 151 45 38 38 CD cord-014901-d9szap94 151 46 ] ] -RRB- cord-014901-d9szap94 151 47 and and CC cord-014901-d9szap94 151 48 trihalose trihalose VBD cord-014901-d9szap94 151 49 in in IN cord-014901-d9szap94 151 50 tobacco tobacco NN cord-014901-d9szap94 151 51 cells cell NNS cord-014901-d9szap94 151 52 [ [ -LRB- cord-014901-d9szap94 151 53 40 40 CD cord-014901-d9szap94 151 54 ] ] -RRB- cord-014901-d9szap94 151 55 . . . cord-014901-d9szap94 152 1 On on IN cord-014901-d9szap94 152 2 the the DT cord-014901-d9szap94 152 3 basis basis NN cord-014901-d9szap94 152 4 of of IN cord-014901-d9szap94 152 5 the the DT cord-014901-d9szap94 152 6 above above JJ cord-014901-d9szap94 152 7 works work NNS cord-014901-d9szap94 152 8 , , , cord-014901-d9szap94 152 9 it -PRON- PRP cord-014901-d9szap94 152 10 becomes become VBZ cord-014901-d9szap94 152 11 appar appar NNP cord-014901-d9szap94 152 12 ent ent NNP cord-014901-d9szap94 152 13 that that IN cord-014901-d9szap94 152 14 transplastome transplastome NNP cord-014901-d9szap94 152 15 plants plant NNS cord-014901-d9szap94 152 16 can can MD cord-014901-d9szap94 152 17 be be VB cord-014901-d9szap94 152 18 regarded regard VBN cord-014901-d9szap94 152 19 as as IN cord-014901-d9szap94 152 20 the the DT cord-014901-d9szap94 152 21 most most RBS cord-014901-d9szap94 152 22 promising promising JJ cord-014901-d9szap94 152 23 system system NN cord-014901-d9szap94 152 24 for for IN cord-014901-d9szap94 152 25 efficient efficient JJ cord-014901-d9szap94 152 26 production production NN cord-014901-d9szap94 152 27 of of IN cord-014901-d9szap94 152 28 pro pro NNP cord-014901-d9szap94 152 29 tein tein NNP cord-014901-d9szap94 152 30 antigens antigen NNS cord-014901-d9szap94 152 31 . . . cord-014901-d9szap94 153 1 However however RB cord-014901-d9szap94 153 2 , , , cord-014901-d9szap94 153 3 the the DT cord-014901-d9szap94 153 4 main main JJ cord-014901-d9szap94 153 5 disadvantage disadvantage NN cord-014901-d9szap94 153 6 of of IN cord-014901-d9szap94 153 7 such such JJ cord-014901-d9szap94 153 8 expression expression NN cord-014901-d9szap94 153 9 system system NN cord-014901-d9szap94 153 10 at at IN cord-014901-d9szap94 153 11 plant plant NN cord-014901-d9szap94 153 12 vaccine vaccine NN cord-014901-d9szap94 153 13 manufacturing manufacturing NN cord-014901-d9szap94 153 14 is be VBZ cord-014901-d9szap94 153 15 that that IN cord-014901-d9szap94 153 16 chloroplasts chloroplast NNS cord-014901-d9szap94 153 17 can can MD cord-014901-d9szap94 153 18 not not RB cord-014901-d9szap94 153 19 glycosylate glycosylate JJ cord-014901-d9szap94 153 20 proteins protein NNS cord-014901-d9szap94 153 21 . . . cord-014901-d9szap94 154 1 At at IN cord-014901-d9szap94 154 2 present present NN cord-014901-d9szap94 154 3 , , , cord-014901-d9szap94 154 4 the the DT cord-014901-d9szap94 154 5 creation creation NN cord-014901-d9szap94 154 6 of of IN cord-014901-d9szap94 154 7 transplastome transplastome JJ cord-014901-d9szap94 154 8 plants plant NNS cord-014901-d9szap94 154 9 is be VBZ cord-014901-d9szap94 154 10 also also RB cord-014901-d9szap94 154 11 associated associate VBN cord-014901-d9szap94 154 12 with with IN cord-014901-d9szap94 154 13 some some DT cord-014901-d9szap94 154 14 technological technological JJ cord-014901-d9szap94 154 15 problems problem NNS cord-014901-d9szap94 154 16 related relate VBN cord-014901-d9szap94 154 17 to to IN cord-014901-d9szap94 154 18 the the DT cord-014901-d9szap94 154 19 absence absence NN cord-014901-d9szap94 154 20 of of IN cord-014901-d9szap94 154 21 the the DT cord-014901-d9szap94 154 22 efficient efficient JJ cord-014901-d9szap94 154 23 system system NN cord-014901-d9szap94 154 24 of of IN cord-014901-d9szap94 154 25 regeneration regeneration NN cord-014901-d9szap94 154 26 for for IN cord-014901-d9szap94 154 27 most most JJS cord-014901-d9szap94 154 28 plant plant NN cord-014901-d9szap94 154 29 species specie NNS cord-014901-d9szap94 154 30 and and CC cord-014901-d9szap94 154 31 also also RB cord-014901-d9szap94 154 32 with with IN cord-014901-d9szap94 154 33 the the DT cord-014901-d9szap94 154 34 absence absence NN cord-014901-d9szap94 154 35 of of IN cord-014901-d9szap94 154 36 the the DT cord-014901-d9szap94 154 37 efficient efficient JJ cord-014901-d9szap94 154 38 system system NN cord-014901-d9szap94 154 39 of of IN cord-014901-d9szap94 154 40 transgen transgen NNP cord-014901-d9szap94 154 41 delivery delivery NN cord-014901-d9szap94 154 42 to to IN cord-014901-d9szap94 154 43 the the DT cord-014901-d9szap94 154 44 chloroplast chloroplast NN cord-014901-d9szap94 154 45 genome genome NN cord-014901-d9szap94 154 46 providing provide VBG cord-014901-d9szap94 154 47 for for IN cord-014901-d9szap94 154 48 the the DT cord-014901-d9szap94 154 49 high high JJ cord-014901-d9szap94 154 50 percent percent NN cord-014901-d9szap94 154 51 of of IN cord-014901-d9szap94 154 52 transformed transform VBN cord-014901-d9szap94 154 53 plant plant NN cord-014901-d9szap94 154 54 yield yield NN cord-014901-d9szap94 154 55 . . . cord-014901-d9szap94 155 1 The the DT cord-014901-d9szap94 155 2 suspension suspension NN cord-014901-d9szap94 155 3 cell cell NN cord-014901-d9szap94 155 4 cultures culture NNS cord-014901-d9szap94 155 5 on on IN cord-014901-d9szap94 155 6 the the DT cord-014901-d9szap94 155 7 basis basis NN cord-014901-d9szap94 155 8 of of IN cord-014901-d9szap94 155 9 geneti geneti NNP cord-014901-d9szap94 155 10 cally cally RB cord-014901-d9szap94 155 11 modified modify VBD cord-014901-d9szap94 155 12 plants plant NNS cord-014901-d9szap94 155 13 attract attract VBP cord-014901-d9szap94 155 14 the the DT cord-014901-d9szap94 155 15 attention attention NN cord-014901-d9szap94 155 16 of of IN cord-014901-d9szap94 155 17 research research NN cord-014901-d9szap94 155 18 ers er NNS cord-014901-d9szap94 155 19 as as IN cord-014901-d9szap94 155 20 promising promising JJ cord-014901-d9szap94 155 21 potential potential JJ cord-014901-d9szap94 155 22 systems system NNS cord-014901-d9szap94 155 23 for for IN cord-014901-d9szap94 155 24 biopharmaceu biopharmaceu NNP cord-014901-d9szap94 155 25 tics tics NNPS cord-014901-d9szap94 155 26 production production NN cord-014901-d9szap94 155 27 . . . cord-014901-d9szap94 156 1 Such such JJ cord-014901-d9szap94 156 2 cultures culture NNS cord-014901-d9szap94 156 3 can can MD cord-014901-d9szap94 156 4 be be VB cord-014901-d9szap94 156 5 obtained obtain VBN cord-014901-d9szap94 156 6 from from IN cord-014901-d9szap94 156 7 loose loose JJ cord-014901-d9szap94 156 8 callus callus NN cord-014901-d9szap94 156 9 tissues tissue NNS cord-014901-d9szap94 156 10 induced induce VBN cord-014901-d9szap94 156 11 from from IN cord-014901-d9szap94 156 12 genetically genetically RB cord-014901-d9szap94 156 13 modified modify VBN cord-014901-d9szap94 156 14 explants explant NNS cord-014901-d9szap94 156 15 or or CC cord-014901-d9szap94 156 16 on on IN cord-014901-d9szap94 156 17 the the DT cord-014901-d9szap94 156 18 basis basis NN cord-014901-d9szap94 156 19 of of IN cord-014901-d9szap94 156 20 co co NN cord-014901-d9szap94 156 21 culturing culturing NN cord-014901-d9szap94 156 22 of of IN cord-014901-d9szap94 156 23 the the DT cord-014901-d9szap94 156 24 cell cell NN cord-014901-d9szap94 156 25 sus sus NNP cord-014901-d9szap94 156 26 pension pension NNP cord-014901-d9szap94 156 27 and and CC cord-014901-d9szap94 156 28 A. A. NNP cord-014901-d9szap94 156 29 tumefasciens tumefasciens NNP cord-014901-d9szap94 156 30 . . . cord-014901-d9szap94 157 1 After after IN cord-014901-d9szap94 157 2 the the DT cord-014901-d9szap94 157 3 assessment assessment NN cord-014901-d9szap94 157 4 of of IN cord-014901-d9szap94 157 5 growth growth NN cord-014901-d9szap94 157 6 characteristics characteristic NNS cord-014901-d9szap94 157 7 and and CC cord-014901-d9szap94 157 8 cell cell NN cord-014901-d9szap94 157 9 line line NN cord-014901-d9szap94 157 10 screening screening NN cord-014901-d9szap94 157 11 to to TO cord-014901-d9szap94 157 12 obtain obtain VB cord-014901-d9szap94 157 13 promising promising JJ cord-014901-d9szap94 157 14 lines line NNS cord-014901-d9szap94 157 15 capable capable JJ cord-014901-d9szap94 157 16 of of IN cord-014901-d9szap94 157 17 the the DT cord-014901-d9szap94 157 18 accumulation accumulation NN cord-014901-d9szap94 157 19 of of IN cord-014901-d9szap94 157 20 great great JJ cord-014901-d9szap94 157 21 amounts amount NNS cord-014901-d9szap94 157 22 of of IN cord-014901-d9szap94 157 23 recombinant recombinant JJ cord-014901-d9szap94 157 24 proteins protein NNS cord-014901-d9szap94 157 25 , , , cord-014901-d9szap94 157 26 such such JJ cord-014901-d9szap94 157 27 lines line NNS cord-014901-d9szap94 157 28 can can MD cord-014901-d9szap94 157 29 be be VB cord-014901-d9szap94 157 30 cultivated cultivate VBN cord-014901-d9szap94 157 31 in in IN cord-014901-d9szap94 157 32 bioreactors bioreactor NNS cord-014901-d9szap94 157 33 for for IN cord-014901-d9szap94 157 34 target target NN cord-014901-d9szap94 157 35 protein protein NN cord-014901-d9szap94 157 36 obtaining obtain VBG cord-014901-d9szap94 157 37 . . . cord-014901-d9szap94 158 1 An an DT cord-014901-d9szap94 158 2 attractive attractive JJ cord-014901-d9szap94 158 3 feature feature NN cord-014901-d9szap94 158 4 of of IN cord-014901-d9szap94 158 5 the the DT cord-014901-d9szap94 158 6 cell cell NN cord-014901-d9szap94 158 7 cultures culture NNS cord-014901-d9szap94 158 8 as as IN cord-014901-d9szap94 158 9 expres expres NNP cord-014901-d9szap94 158 10 sion sion NNP cord-014901-d9szap94 158 11 systems system NNS cord-014901-d9szap94 158 12 for for IN cord-014901-d9szap94 158 13 recombinant recombinant JJ cord-014901-d9szap94 158 14 protein protein NN cord-014901-d9szap94 158 15 production production NN cord-014901-d9szap94 158 16 , , , cord-014901-d9szap94 158 17 as as IN cord-014901-d9szap94 158 18 compared compare VBN cord-014901-d9szap94 158 19 to to IN cord-014901-d9szap94 158 20 the the DT cord-014901-d9szap94 158 21 use use NN cord-014901-d9szap94 158 22 of of IN cord-014901-d9szap94 158 23 whole whole JJ cord-014901-d9szap94 158 24 plants plant NNS cord-014901-d9szap94 158 25 for for IN cord-014901-d9szap94 158 26 this this DT cord-014901-d9szap94 158 27 purpose purpose NN cord-014901-d9szap94 158 28 , , , cord-014901-d9szap94 158 29 is be VBZ cord-014901-d9szap94 158 30 that that DT cord-014901-d9szap94 158 31 cell cell NN cord-014901-d9szap94 158 32 culture culture NN cord-014901-d9szap94 158 33 can can MD cord-014901-d9szap94 158 34 be be VB cord-014901-d9szap94 158 35 unified unify VBN cord-014901-d9szap94 158 36 in in IN cord-014901-d9szap94 158 37 growth growth NN cord-014901-d9szap94 158 38 character character NN cord-014901-d9szap94 158 39 istics istic NNS cord-014901-d9szap94 158 40 , , , cord-014901-d9szap94 158 41 cell cell NN cord-014901-d9szap94 158 42 dimensions dimension NNS cord-014901-d9szap94 158 43 and and CC cord-014901-d9szap94 158 44 types type NNS cord-014901-d9szap94 158 45 . . . cord-014901-d9szap94 159 1 Moreover moreover RB cord-014901-d9szap94 159 2 , , , cord-014901-d9szap94 159 3 cells cell NNS cord-014901-d9szap94 159 4 are be VBP cord-014901-d9szap94 159 5 grown grow VBN cord-014901-d9szap94 159 6 under under IN cord-014901-d9szap94 159 7 strictly strictly RB cord-014901-d9szap94 159 8 controlled control VBN cord-014901-d9szap94 159 9 conditions condition NNS cord-014901-d9szap94 159 10 , , , cord-014901-d9szap94 159 11 when when WRB cord-014901-d9szap94 159 12 the the DT cord-014901-d9szap94 159 13 product product NN cord-014901-d9szap94 159 14 accumulation accumulation NN cord-014901-d9szap94 159 15 does do VBZ cord-014901-d9szap94 159 16 not not RB cord-014901-d9szap94 159 17 depend depend VB cord-014901-d9szap94 159 18 on on IN cord-014901-d9szap94 159 19 the the DT cord-014901-d9szap94 159 20 sea sea NN cord-014901-d9szap94 159 21 sonal sonal JJ cord-014901-d9szap94 159 22 weather weather NN cord-014901-d9szap94 159 23 changes change NNS cord-014901-d9szap94 159 24 and and CC cord-014901-d9szap94 159 25 allows allow VBZ cord-014901-d9szap94 159 26 the the DT cord-014901-d9szap94 159 27 permanent permanent JJ cord-014901-d9szap94 159 28 product product NN cord-014901-d9szap94 159 29 obtaining obtain VBG cord-014901-d9szap94 159 30 in in IN cord-014901-d9szap94 159 31 bioreactors bioreactor NNS cord-014901-d9szap94 159 32 . . . cord-014901-d9szap94 160 1 The the DT cord-014901-d9szap94 160 2 additional additional JJ cord-014901-d9szap94 160 3 insertion insertion NN cord-014901-d9szap94 160 4 of of IN cord-014901-d9szap94 160 5 signal signal NN cord-014901-d9szap94 160 6 peptide peptide NN cord-014901-d9szap94 160 7 nucleotide nucleotide JJ cord-014901-d9szap94 160 8 sequences sequence NNS cord-014901-d9szap94 160 9 into into IN cord-014901-d9szap94 160 10 the the DT cord-014901-d9szap94 160 11 construct construct NN cord-014901-d9szap94 160 12 permits permit VBZ cord-014901-d9szap94 160 13 a a DT cord-014901-d9szap94 160 14 protein protein NN cord-014901-d9szap94 160 15 secretion secretion NN cord-014901-d9szap94 160 16 into into IN cord-014901-d9szap94 160 17 the the DT cord-014901-d9szap94 160 18 intercellular intercellular JJ cord-014901-d9szap94 160 19 space space NN cord-014901-d9szap94 160 20 , , , cord-014901-d9szap94 160 21 which which WDT cord-014901-d9szap94 160 22 allows allow VBZ cord-014901-d9szap94 160 23 target target NN cord-014901-d9szap94 160 24 protein protein NN cord-014901-d9szap94 160 25 isola isola NNP cord-014901-d9szap94 160 26 tion tion NNP cord-014901-d9szap94 160 27 directly directly RB cord-014901-d9szap94 160 28 from from IN cord-014901-d9szap94 160 29 the the DT cord-014901-d9szap94 160 30 culture culture NN cord-014901-d9szap94 160 31 liquid liquid NN cord-014901-d9szap94 160 32 . . . cord-014901-d9szap94 161 1 The the DT cord-014901-d9szap94 161 2 addition addition NN cord-014901-d9szap94 161 3 of of IN cord-014901-d9szap94 161 4 recombinant recombinant JJ cord-014901-d9szap94 161 5 protein protein NN cord-014901-d9szap94 161 6 stabilizers stabilizer NNS cord-014901-d9szap94 161 7 to to IN cord-014901-d9szap94 161 8 the the DT cord-014901-d9szap94 161 9 suspension suspension NN cord-014901-d9szap94 161 10 cul cul NN cord-014901-d9szap94 161 11 ture ture NN cord-014901-d9szap94 161 12 increases increase VBZ cord-014901-d9szap94 161 13 the the DT cord-014901-d9szap94 161 14 yield yield NN cord-014901-d9szap94 161 15 of of IN cord-014901-d9szap94 161 16 the the DT cord-014901-d9szap94 161 17 target target NN cord-014901-d9szap94 161 18 protein protein NN cord-014901-d9szap94 161 19 [ [ -LRB- cord-014901-d9szap94 161 20 41 41 CD cord-014901-d9szap94 161 21 ] ] -RRB- cord-014901-d9szap94 161 22 . . . cord-014901-d9szap94 162 1 By by IN cord-014901-d9szap94 162 2 their -PRON- PRP$ cord-014901-d9szap94 162 3 capabilities capability NNS cord-014901-d9szap94 162 4 , , , cord-014901-d9szap94 162 5 plant plant NN cord-014901-d9szap94 162 6 cell cell NN cord-014901-d9szap94 162 7 cultures culture NNS cord-014901-d9szap94 162 8 are be VBP cord-014901-d9szap94 162 9 compa compa NNP cord-014901-d9szap94 162 10 rable rable FW cord-014901-d9szap94 162 11 in in IN cord-014901-d9szap94 162 12 production production NN cord-014901-d9szap94 162 13 of of IN cord-014901-d9szap94 162 14 therapeutic therapeutic JJ cord-014901-d9szap94 162 15 recombinant recombinant JJ cord-014901-d9szap94 162 16 pro pro FW cord-014901-d9szap94 162 17 teins tein NNS cord-014901-d9szap94 162 18 with with IN cord-014901-d9szap94 162 19 the the DT cord-014901-d9szap94 162 20 conventionally conventionally RB cord-014901-d9szap94 162 21 used use VBN cord-014901-d9szap94 162 22 mammalian mammalian JJ cord-014901-d9szap94 162 23 cell cell NN cord-014901-d9szap94 162 24 cultures culture NNS cord-014901-d9szap94 162 25 , , , cord-014901-d9szap94 162 26 such such JJ cord-014901-d9szap94 162 27 as as IN cord-014901-d9szap94 162 28 Chinese chinese JJ cord-014901-d9szap94 162 29 hamster hamster NN cord-014901-d9szap94 162 30 ovary ovary NN cord-014901-d9szap94 162 31 cells cell NNS cord-014901-d9szap94 162 32 . . . cord-014901-d9szap94 163 1 How how WRB cord-014901-d9szap94 163 2 ever ever RB cord-014901-d9szap94 163 3 , , , cord-014901-d9szap94 163 4 as as IN cord-014901-d9szap94 163 5 distinct distinct JJ cord-014901-d9szap94 163 6 from from IN cord-014901-d9szap94 163 7 the the DT cord-014901-d9szap94 163 8 mammal mammal NN cord-014901-d9szap94 163 9 suspension suspension NN cord-014901-d9szap94 163 10 cultures culture NNS cord-014901-d9szap94 163 11 , , , cord-014901-d9szap94 163 12 they -PRON- PRP cord-014901-d9szap94 163 13 are be VBP cord-014901-d9szap94 163 14 not not RB cord-014901-d9szap94 163 15 infected infect VBN cord-014901-d9szap94 163 16 by by IN cord-014901-d9szap94 163 17 any any DT cord-014901-d9szap94 163 18 animal animal NN cord-014901-d9szap94 163 19 pathogens pathogen NNS cord-014901-d9szap94 163 20 . . . cord-014901-d9szap94 164 1 To to IN cord-014901-d9szap94 164 2 data datum NNS cord-014901-d9szap94 164 3 , , , cord-014901-d9szap94 164 4 there there EX cord-014901-d9szap94 164 5 are be VBP cord-014901-d9szap94 164 6 many many JJ cord-014901-d9szap94 164 7 examples example NNS cord-014901-d9szap94 164 8 of of IN cord-014901-d9szap94 164 9 plant plant NN cord-014901-d9szap94 164 10 cell cell NN cord-014901-d9szap94 164 11 cultures culture NNS cord-014901-d9szap94 164 12 with with IN cord-014901-d9szap94 164 13 the the DT cord-014901-d9szap94 164 14 yield yield NN cord-014901-d9szap94 164 15 of of IN cord-014901-d9szap94 164 16 recombinant recombinant JJ cord-014901-d9szap94 164 17 protein protein NN cord-014901-d9szap94 164 18 in in IN cord-014901-d9szap94 164 19 the the DT cord-014901-d9szap94 164 20 amount amount NN cord-014901-d9szap94 164 21 more more JJR cord-014901-d9szap94 164 22 than than IN cord-014901-d9szap94 164 23 10 10 CD cord-014901-d9szap94 164 24 mg mg NNP cord-014901-d9szap94 164 25 / / SYM cord-014901-d9szap94 164 26 L L NNP cord-014901-d9szap94 164 27 , , , cord-014901-d9szap94 164 28 which which WDT cord-014901-d9szap94 164 29 is be VBZ cord-014901-d9szap94 164 30 a a DT cord-014901-d9szap94 164 31 threshold threshold NN cord-014901-d9szap94 164 32 value value NN cord-014901-d9szap94 164 33 for for IN cord-014901-d9szap94 164 34 starting start VBG cord-014901-d9szap94 164 35 the the DT cord-014901-d9szap94 164 36 commercial commercial JJ cord-014901-d9szap94 164 37 manufacture manufacture NN cord-014901-d9szap94 164 38 of of IN cord-014901-d9szap94 164 39 the the DT cord-014901-d9szap94 164 40 product product NN cord-014901-d9szap94 164 41 [ [ -LRB- cord-014901-d9szap94 164 42 42 42 CD cord-014901-d9szap94 164 43 ] ] -RRB- cord-014901-d9szap94 164 44 . . . cord-014901-d9szap94 165 1 An an DT cord-014901-d9szap94 165 2 example example NN cord-014901-d9szap94 165 3 of of IN cord-014901-d9szap94 165 4 a a DT cord-014901-d9szap94 165 5 commercially commercially RB cord-014901-d9szap94 165 6 successful successful JJ cord-014901-d9szap94 165 7 produc produc NN cord-014901-d9szap94 165 8 tion tion NN cord-014901-d9szap94 165 9 of of IN cord-014901-d9szap94 165 10 veterinary veterinary JJ cord-014901-d9szap94 165 11 vaccine vaccine NN cord-014901-d9szap94 165 12 products product NNS cord-014901-d9szap94 165 13 is be VBZ cord-014901-d9szap94 165 14 developed develop VBN cord-014901-d9szap94 165 15 by by IN cord-014901-d9szap94 165 16 Dow Dow NNP cord-014901-d9szap94 165 17 Agro Agro NNP cord-014901-d9szap94 165 18 Sciences Sciences NNPS cord-014901-d9szap94 165 19 company company NN cord-014901-d9szap94 165 20 ( ( -LRB- cord-014901-d9szap94 165 21 United United NNP cord-014901-d9szap94 165 22 States States NNP cord-014901-d9szap94 165 23 ) ) -RRB- cord-014901-d9szap94 165 24 system system NN cord-014901-d9szap94 165 25 Concert Concert NNP cord-014901-d9szap94 165 26 ™ ™ NN cord-014901-d9szap94 165 27 , , , cord-014901-d9szap94 165 28 patented patent VBN cord-014901-d9szap94 165 29 as as IN cord-014901-d9szap94 165 30 an an DT cord-014901-d9szap94 165 31 effective effective JJ cord-014901-d9szap94 165 32 and and CC cord-014901-d9szap94 165 33 safe safe JJ cord-014901-d9szap94 165 34 system system NN cord-014901-d9szap94 165 35 for for IN cord-014901-d9szap94 165 36 the the DT cord-014901-d9szap94 165 37 production production NN cord-014901-d9szap94 165 38 of of IN cord-014901-d9szap94 165 39 vaccine vaccine NN cord-014901-d9szap94 165 40 proteins protein NNS cord-014901-d9szap94 165 41 in in IN cord-014901-d9szap94 165 42 cultured cultured JJ cord-014901-d9szap94 165 43 plant plant NN cord-014901-d9szap94 165 44 cells cell NNS cord-014901-d9szap94 165 45 , , , cord-014901-d9szap94 165 46 cultured culture VBN cord-014901-d9szap94 165 47 in in IN cord-014901-d9szap94 165 48 a a DT cord-014901-d9szap94 165 49 bioreactor bioreactor NN cord-014901-d9szap94 165 50 . . . cord-014901-d9szap94 166 1 The the DT cord-014901-d9szap94 166 2 first first JJ cord-014901-d9szap94 166 3 plant plant NN cord-014901-d9szap94 166 4 vaccine vaccine NN cord-014901-d9szap94 166 5 against against IN cord-014901-d9szap94 166 6 Newcastle Newcastle NNP cord-014901-d9szap94 166 7 disease disease NN cord-014901-d9szap94 166 8 virus virus NN cord-014901-d9szap94 166 9 of of IN cord-014901-d9szap94 166 10 birds bird NNS cord-014901-d9szap94 166 11 obtained obtain VBN cord-014901-d9szap94 166 12 in in IN cord-014901-d9szap94 166 13 the the DT cord-014901-d9szap94 166 14 tobacco tobacco NN cord-014901-d9szap94 166 15 cell cell NN cord-014901-d9szap94 166 16 culture culture NN cord-014901-d9szap94 166 17 was be VBD cord-014901-d9szap94 166 18 approved approve VBN cord-014901-d9szap94 166 19 for for IN cord-014901-d9szap94 166 20 use use NN cord-014901-d9szap94 166 21 by by IN cord-014901-d9szap94 166 22 the the DT cord-014901-d9szap94 166 23 Min Min NNP cord-014901-d9szap94 166 24 istry istry NN cord-014901-d9szap94 166 25 of of IN cord-014901-d9szap94 166 26 Agriculture Agriculture NNP cord-014901-d9szap94 166 27 of of IN cord-014901-d9szap94 166 28 the the DT cord-014901-d9szap94 166 29 United United NNP cord-014901-d9szap94 166 30 States States NNP cord-014901-d9szap94 166 31 in in IN cord-014901-d9szap94 166 32 2006 2006 CD cord-014901-d9szap94 166 33 . . . cord-014901-d9szap94 167 1 The the DT cord-014901-d9szap94 167 2 disadvantages disadvantage NNS cord-014901-d9szap94 167 3 of of IN cord-014901-d9szap94 167 4 this this DT cord-014901-d9szap94 167 5 expression expression NN cord-014901-d9szap94 167 6 system system NN cord-014901-d9szap94 167 7 are be VBP cord-014901-d9szap94 167 8 still still RB cord-014901-d9szap94 167 9 insufficiently insufficiently RB cord-014901-d9szap94 167 10 high high JJ cord-014901-d9szap94 167 11 yield yield NN cord-014901-d9szap94 167 12 of of IN cord-014901-d9szap94 167 13 the the DT cord-014901-d9szap94 167 14 target target NN cord-014901-d9szap94 167 15 recombinant recombinant JJ cord-014901-d9szap94 167 16 pro pro FW cord-014901-d9szap94 167 17 tein tein NN cord-014901-d9szap94 167 18 and and CC cord-014901-d9szap94 167 19 the the DT cord-014901-d9szap94 167 20 instability instability NN cord-014901-d9szap94 167 21 of of IN cord-014901-d9szap94 167 22 foreign foreign JJ cord-014901-d9szap94 167 23 genes gene NNS cord-014901-d9szap94 167 24 in in IN cord-014901-d9szap94 167 25 cultured cultured JJ cord-014901-d9szap94 167 26 plant plant NN cord-014901-d9szap94 167 27 cells cell NNS cord-014901-d9szap94 167 28 due due JJ cord-014901-d9szap94 167 29 to to IN cord-014901-d9szap94 167 30 the the DT cord-014901-d9szap94 167 31 epigenetic epigenetic JJ cord-014901-d9szap94 167 32 silencing silencing NN cord-014901-d9szap94 167 33 of of IN cord-014901-d9szap94 167 34 the the DT cord-014901-d9szap94 167 35 trans trans NNP cord-014901-d9szap94 167 36 gene gene NN cord-014901-d9szap94 167 37 transcription transcription NN cord-014901-d9szap94 167 38 [ [ -LRB- cord-014901-d9szap94 167 39 43 43 CD cord-014901-d9szap94 167 40 ] ] -RRB- cord-014901-d9szap94 167 41 . . . cord-014901-d9szap94 168 1 Advantages advantage NNS cord-014901-d9szap94 168 2 , , , cord-014901-d9szap94 168 3 disadvantages disadvantage NNS cord-014901-d9szap94 168 4 , , , cord-014901-d9szap94 168 5 and and CC cord-014901-d9szap94 168 6 specific specific JJ cord-014901-d9szap94 168 7 features feature NNS cord-014901-d9szap94 168 8 of of IN cord-014901-d9szap94 168 9 recombinant recombinant JJ cord-014901-d9szap94 168 10 protein protein NN cord-014901-d9szap94 168 11 produc produc NN cord-014901-d9szap94 168 12 tion tion NN cord-014901-d9szap94 168 13 in in IN cord-014901-d9szap94 168 14 the the DT cord-014901-d9szap94 168 15 plant plant NN cord-014901-d9szap94 168 16 cell cell NN cord-014901-d9szap94 168 17 cultures culture NNS cord-014901-d9szap94 168 18 are be VBP cord-014901-d9szap94 168 19 discussed discuss VBN cord-014901-d9szap94 168 20 in in IN cord-014901-d9szap94 168 21 reviews review NNS cord-014901-d9szap94 168 22 [ [ -LRB- cord-014901-d9szap94 168 23 42 42 CD cord-014901-d9szap94 168 24 , , , cord-014901-d9szap94 168 25 44 44 CD cord-014901-d9szap94 168 26 ] ] -RRB- cord-014901-d9szap94 168 27 . . . cord-014901-d9szap94 169 1 There there EX cord-014901-d9szap94 169 2 are be VBP cord-014901-d9szap94 169 3 many many JJ cord-014901-d9szap94 169 4 examples example NNS cord-014901-d9szap94 169 5 of of IN cord-014901-d9szap94 169 6 successful successful JJ cord-014901-d9szap94 169 7 use use NN cord-014901-d9szap94 169 8 of of IN cord-014901-d9szap94 169 9 aqueous aqueous JJ cord-014901-d9szap94 169 10 plants plant NNS cord-014901-d9szap94 169 11 , , , cord-014901-d9szap94 169 12 such such JJ cord-014901-d9szap94 169 13 as as IN cord-014901-d9szap94 169 14 duckweed duckweed NN cord-014901-d9szap94 169 15 , , , cord-014901-d9szap94 169 16 unicellular unicellular JJ cord-014901-d9szap94 169 17 algae algae NN cord-014901-d9szap94 169 18 , , , cord-014901-d9szap94 169 19 and and CC cord-014901-d9szap94 169 20 mosses moss NNS cord-014901-d9szap94 169 21 , , , cord-014901-d9szap94 169 22 which which WDT cord-014901-d9szap94 169 23 are be VBP cord-014901-d9szap94 169 24 cultivated cultivate VBN cord-014901-d9szap94 169 25 similarly similarly RB cord-014901-d9szap94 169 26 as as IN cord-014901-d9szap94 169 27 plant plant NN cord-014901-d9szap94 169 28 cell cell NN cord-014901-d9szap94 169 29 suspensions suspension NNS cord-014901-d9szap94 169 30 in in IN cord-014901-d9szap94 169 31 bioreactors bioreactor NNS cord-014901-d9szap94 169 32 , , , cord-014901-d9szap94 169 33 for for IN cord-014901-d9szap94 169 34 recombinant recombinant JJ cord-014901-d9szap94 169 35 pro pro FW cord-014901-d9szap94 169 36 tein tein NNP cord-014901-d9szap94 169 37 expression expression NN cord-014901-d9szap94 169 38 . . . cord-014901-d9szap94 170 1 Duckweed duckweed NN cord-014901-d9szap94 170 2 attracts attract VBZ cord-014901-d9szap94 170 3 the the DT cord-014901-d9szap94 170 4 attention attention NN cord-014901-d9szap94 170 5 of of IN cord-014901-d9szap94 170 6 researchers researcher NNS cord-014901-d9szap94 170 7 as as IN cord-014901-d9szap94 170 8 a a DT cord-014901-d9szap94 170 9 potential potential NN cord-014901-d9szap94 170 10 highly highly RB cord-014901-d9szap94 170 11 efficient efficient JJ cord-014901-d9szap94 170 12 system system NN cord-014901-d9szap94 170 13 for for IN cord-014901-d9szap94 170 14 recombinant recombinant JJ cord-014901-d9szap94 170 15 pro pro FW cord-014901-d9szap94 170 16 tein tein NNP cord-014901-d9szap94 170 17 expression expression NN cord-014901-d9szap94 170 18 due due IN cord-014901-d9szap94 170 19 to to IN cord-014901-d9szap94 170 20 its -PRON- PRP$ cord-014901-d9szap94 170 21 capability capability NN cord-014901-d9szap94 170 22 of of IN cord-014901-d9szap94 170 23 a a DT cord-014901-d9szap94 170 24 rapid rapid JJ cord-014901-d9szap94 170 25 biomass biomass NN cord-014901-d9szap94 170 26 accumulation accumulation NN cord-014901-d9szap94 170 27 : : : cord-014901-d9szap94 170 28 it -PRON- PRP cord-014901-d9szap94 170 29 can can MD cord-014901-d9szap94 170 30 be be VB cord-014901-d9szap94 170 31 doubled double VBN cord-014901-d9szap94 170 32 for for IN cord-014901-d9szap94 170 33 24 24 CD cord-014901-d9szap94 170 34 - - SYM cord-014901-d9szap94 170 35 48 48 CD cord-014901-d9szap94 170 36 h. h. NNP cord-014901-d9szap94 170 37 Geneti Geneti NNP cord-014901-d9szap94 170 38 cally cally RB cord-014901-d9szap94 170 39 modified modify VBD cord-014901-d9szap94 170 40 duckweed duckweed NN cord-014901-d9szap94 170 41 as as IN cord-014901-d9szap94 170 42 a a DT cord-014901-d9szap94 170 43 potential potential JJ cord-014901-d9szap94 170 44 producer producer NN cord-014901-d9szap94 170 45 of of IN cord-014901-d9szap94 170 46 biopharmaceutic biopharmaceutic JJ cord-014901-d9szap94 170 47 proteins protein NNS cord-014901-d9szap94 170 48 can can MD cord-014901-d9szap94 170 49 be be VB cord-014901-d9szap94 170 50 used use VBN cord-014901-d9szap94 170 51 by by IN cord-014901-d9szap94 170 52 animals animal NNS cord-014901-d9szap94 170 53 as as IN cord-014901-d9szap94 170 54 a a DT cord-014901-d9szap94 170 55 raw raw JJ cord-014901-d9szap94 170 56 or or CC cord-014901-d9szap94 170 57 dried dry VBN cord-014901-d9szap94 170 58 food food NN cord-014901-d9szap94 170 59 . . . cord-014901-d9szap94 171 1 Duckweed duckweed NN cord-014901-d9szap94 171 2 is be VBZ cord-014901-d9szap94 171 3 a a DT cord-014901-d9szap94 171 4 monocotyledonous monocotyledonous JJ cord-014901-d9szap94 171 5 angiosperm angiosperm NN cord-014901-d9szap94 171 6 ; ; , cord-014901-d9szap94 171 7 for for IN cord-014901-d9szap94 171 8 foreign foreign JJ cord-014901-d9szap94 171 9 gene gene NN cord-014901-d9szap94 171 10 transfer transfer NN cord-014901-d9szap94 171 11 into into IN cord-014901-d9szap94 171 12 its -PRON- PRP$ cord-014901-d9szap94 171 13 genome genome NN cord-014901-d9szap94 171 14 , , , cord-014901-d9szap94 171 15 the the DT cord-014901-d9szap94 171 16 methods method NNS cord-014901-d9szap94 171 17 of of IN cord-014901-d9szap94 171 18 agrobacterial agrobacterial JJ cord-014901-d9szap94 171 19 transformation transformation NN cord-014901-d9szap94 171 20 and and CC cord-014901-d9szap94 171 21 bioballistics bioballistic NNS cord-014901-d9szap94 171 22 are be VBP cord-014901-d9szap94 171 23 used use VBN cord-014901-d9szap94 171 24 . . . cord-014901-d9szap94 172 1 Examples example NNS cord-014901-d9szap94 172 2 are be VBP cord-014901-d9szap94 172 3 known know VBN cord-014901-d9szap94 172 4 when when WRB cord-014901-d9szap94 172 5 genetically genetically RB cord-014901-d9szap94 172 6 modified modify VBN cord-014901-d9szap94 172 7 duckweed duckweed NN cord-014901-d9szap94 172 8 accumulated accumulate VBN cord-014901-d9szap94 172 9 recombi recombi NNP cord-014901-d9szap94 172 10 nant nant NNP cord-014901-d9szap94 172 11 protein protein NN cord-014901-d9szap94 172 12 in in IN cord-014901-d9szap94 172 13 the the DT cord-014901-d9szap94 172 14 amounts amount NNS cord-014901-d9szap94 172 15 up up IN cord-014901-d9szap94 172 16 to to TO cord-014901-d9szap94 172 17 25 25 CD cord-014901-d9szap94 172 18 % % NN cord-014901-d9szap94 172 19 of of IN cord-014901-d9szap94 172 20 TSP tsp NN cord-014901-d9szap94 172 21 , , , cord-014901-d9szap94 172 22 as as IN cord-014901-d9szap94 172 23 assessed assess VBN cord-014901-d9szap94 172 24 after after IN cord-014901-d9szap94 172 25 the the DT cord-014901-d9szap94 172 26 accumulation accumulation NN cord-014901-d9szap94 172 27 of of IN cord-014901-d9szap94 172 28 GFP GFP NNP cord-014901-d9szap94 172 29 [ [ -LRB- cord-014901-d9szap94 172 30 45 45 CD cord-014901-d9szap94 172 31 ] ] -RRB- cord-014901-d9szap94 172 32 . . . cord-014901-d9szap94 173 1 Sequenc Sequenc NNP cord-014901-d9szap94 173 2 ing e VBG cord-014901-d9szap94 173 3 the the DT cord-014901-d9szap94 173 4 duckweed duckweed NN cord-014901-d9szap94 173 5 chloroplast chloroplast NN cord-014901-d9szap94 173 6 genome genome NN cord-014901-d9szap94 173 7 is be VBZ cord-014901-d9szap94 173 8 close close JJ cord-014901-d9szap94 173 9 to to TO cord-014901-d9szap94 173 10 com com NN cord-014901-d9szap94 173 11 pleting pleting NN cord-014901-d9szap94 173 12 , , , cord-014901-d9szap94 173 13 which which WDT cord-014901-d9szap94 173 14 opens open VBZ cord-014901-d9szap94 173 15 up up RP cord-014901-d9szap94 173 16 some some DT cord-014901-d9szap94 173 17 prospects prospect NNS cord-014901-d9szap94 173 18 for for IN cord-014901-d9szap94 173 19 a a DT cord-014901-d9szap94 173 20 signifi signifi NN cord-014901-d9szap94 173 21 ca can MD cord-014901-d9szap94 173 22 nt not RB cord-014901-d9szap94 173 23 increase increase VB cord-014901-d9szap94 173 24 in in IN cord-014901-d9szap94 173 25 the the DT cord-014901-d9szap94 173 26 yield yield NN cord-014901-d9szap94 173 27 of of IN cord-014901-d9szap94 173 28 recombinant recombinant JJ cord-014901-d9szap94 173 29 proteins protein NNS cord-014901-d9szap94 173 30 . . . cord-014901-d9szap94 174 1 The the DT cord-014901-d9szap94 174 2 systems system NNS cord-014901-d9szap94 174 3 of of IN cord-014901-d9szap94 174 4 biopharmaceuticals biopharmaceutical NNS cord-014901-d9szap94 174 5 production production NN cord-014901-d9szap94 174 6 using use VBG cord-014901-d9szap94 174 7 genetically genetically RB cord-014901-d9szap94 174 8 modified modify VBN cord-014901-d9szap94 174 9 microalgae microalgae NN cord-014901-d9szap94 174 10 are be VBP cord-014901-d9szap94 174 11 actively actively RB cord-014901-d9szap94 174 12 developed develop VBN cord-014901-d9szap94 174 13 . . . cord-014901-d9szap94 175 1 Algae algae NN cord-014901-d9szap94 175 2 combine combine VB cord-014901-d9szap94 175 3 advantages advantage NNS cord-014901-d9szap94 175 4 of of IN cord-014901-d9szap94 175 5 both both DT cord-014901-d9szap94 175 6 bacteria bacteria NNS cord-014901-d9szap94 175 7 ( ( -LRB- cord-014901-d9szap94 175 8 rapid rapid JJ cord-014901-d9szap94 175 9 growth growth NN cord-014901-d9szap94 175 10 and and CC cord-014901-d9szap94 175 11 simplicity simplicity NN cord-014901-d9szap94 175 12 of of IN cord-014901-d9szap94 175 13 cultivation cultivation NN cord-014901-d9szap94 175 14 ) ) -RRB- cord-014901-d9szap94 175 15 and and CC cord-014901-d9szap94 175 16 higher high JJR cord-014901-d9szap94 175 17 plants plant NNS cord-014901-d9szap94 175 18 ( ( -LRB- cord-014901-d9szap94 175 19 a a DT cord-014901-d9szap94 175 20 capability capability NN cord-014901-d9szap94 175 21 of of IN cord-014901-d9szap94 175 22 posttranslational posttranslational JJ cord-014901-d9szap94 175 23 modifications modification NNS cord-014901-d9szap94 175 24 and and CC cord-014901-d9szap94 175 25 photosynthesis photosynthesis NN cord-014901-d9szap94 175 26 ) ) -RRB- cord-014901-d9szap94 175 27 . . . cord-014901-d9szap94 176 1 Chlamydomonas Chlamydomonas NNP cord-014901-d9szap94 176 2 reinhardtii reinhardtii NNP cord-014901-d9szap94 176 3 is be VBZ cord-014901-d9szap94 176 4 most most RBS cord-014901-d9szap94 176 5 promising promising JJ cord-014901-d9szap94 176 6 among among IN cord-014901-d9szap94 176 7 algae algae NN cord-014901-d9szap94 176 8 : : : cord-014901-d9szap94 176 9 it -PRON- PRP cord-014901-d9szap94 176 10 has have VBZ cord-014901-d9szap94 176 11 a a DT cord-014901-d9szap94 176 12 short short JJ cord-014901-d9szap94 176 13 time time NN cord-014901-d9szap94 176 14 of of IN cord-014901-d9szap94 176 15 bio bio NNP cord-014901-d9szap94 176 16 mass mass NNP cord-014901-d9szap94 176 17 doubling doubling NN cord-014901-d9szap94 176 18 ( ( -LRB- cord-014901-d9szap94 176 19 about about RB cord-014901-d9szap94 176 20 10 10 CD cord-014901-d9szap94 176 21 h h NN cord-014901-d9szap94 176 22 ) ) -RRB- cord-014901-d9szap94 176 23 , , , cord-014901-d9szap94 176 24 it -PRON- PRP cord-014901-d9szap94 176 25 is be VBZ cord-014901-d9szap94 176 26 easily easily RB cord-014901-d9szap94 176 27 subjected subject VBN cord-014901-d9szap94 176 28 to to IN cord-014901-d9szap94 176 29 nuclear nuclear JJ cord-014901-d9szap94 176 30 and and CC cord-014901-d9szap94 176 31 chloroplast chloroplast JJ cord-014901-d9szap94 176 32 transformation transformation NN cord-014901-d9szap94 176 33 , , , cord-014901-d9szap94 176 34 it -PRON- PRP cord-014901-d9szap94 176 35 can can MD cord-014901-d9szap94 176 36 be be VB cord-014901-d9szap94 176 37 grown grow VBN cord-014901-d9szap94 176 38 under under IN cord-014901-d9szap94 176 39 photoautotrophic photoautotrophic JJ cord-014901-d9szap94 176 40 conditions condition NNS cord-014901-d9szap94 176 41 or or CC cord-014901-d9szap94 176 42 with with IN cord-014901-d9szap94 176 43 the the DT cord-014901-d9szap94 176 44 addition addition NN cord-014901-d9szap94 176 45 of of IN cord-014901-d9szap94 176 46 acetate acetate NN cord-014901-d9szap94 176 47 as as IN cord-014901-d9szap94 176 48 the the DT cord-014901-d9szap94 176 49 source source NN cord-014901-d9szap94 176 50 of of IN cord-014901-d9szap94 176 51 carbon carbon NN cord-014901-d9szap94 176 52 . . . cord-014901-d9szap94 177 1 Nuclear nuclear JJ cord-014901-d9szap94 177 2 transformants transformant NNS cord-014901-d9szap94 177 3 usually usually RB cord-014901-d9szap94 177 4 give give VBP cord-014901-d9szap94 177 5 rather rather RB cord-014901-d9szap94 177 6 low low JJ cord-014901-d9szap94 177 7 yield yield NN cord-014901-d9szap94 177 8 of of IN cord-014901-d9szap94 177 9 protein protein NN cord-014901-d9szap94 177 10 product product NN cord-014901-d9szap94 177 11 ; ; : cord-014901-d9szap94 177 12 therefore therefore RB cord-014901-d9szap94 177 13 , , , cord-014901-d9szap94 177 14 recombinant recombinant JJ cord-014901-d9szap94 177 15 protein protein NN cord-014901-d9szap94 177 16 production production NN cord-014901-d9szap94 177 17 by by IN cord-014901-d9szap94 177 18 this this DT cord-014901-d9szap94 177 19 alga alga NN cord-014901-d9szap94 177 20 is be VBZ cord-014901-d9szap94 177 21 based base VBN cord-014901-d9szap94 177 22 on on IN cord-014901-d9szap94 177 23 the the DT cord-014901-d9szap94 177 24 transformation transformation NN cord-014901-d9szap94 177 25 of of IN cord-014901-d9szap94 177 26 chloro chloro NNP cord-014901-d9szap94 177 27 No no NN cord-014901-d9szap94 177 28 . . NN cord-014901-d9szap94 177 29 1 1 CD cord-014901-d9szap94 177 30 2015 2015 CD cord-014901-d9szap94 177 31 plast plast NNP cord-014901-d9szap94 177 32 , , , cord-014901-d9szap94 177 33 which which WDT cord-014901-d9szap94 177 34 occupies occupy VBZ cord-014901-d9szap94 177 35 about about IN cord-014901-d9szap94 177 36 40 40 CD cord-014901-d9szap94 177 37 % % NN cord-014901-d9szap94 177 38 of of IN cord-014901-d9szap94 177 39 the the DT cord-014901-d9szap94 177 40 cell cell NN cord-014901-d9szap94 177 41 volume volume NN cord-014901-d9szap94 177 42 [ [ -LRB- cord-014901-d9szap94 177 43 46 46 CD cord-014901-d9szap94 177 44 ] ] -RRB- cord-014901-d9szap94 177 45 . . . cord-014901-d9szap94 178 1 C. C. NNP cord-014901-d9szap94 178 2 reinhardtii reinhardtii NNP cord-014901-d9szap94 178 3 nuclear nuclear NN cord-014901-d9szap94 178 4 and and CC cord-014901-d9szap94 178 5 chloroplast chloroplast NN cord-014901-d9szap94 178 6 genomes genome NNS cord-014901-d9szap94 178 7 are be VBP cord-014901-d9szap94 178 8 sequenced sequence VBN cord-014901-d9szap94 178 9 , , , cord-014901-d9szap94 178 10 and and CC cord-014901-d9szap94 178 11 this this DT cord-014901-d9szap94 178 12 simplifies simplify VBZ cord-014901-d9szap94 178 13 substantially substantially RB cord-014901-d9szap94 178 14 any any DT cord-014901-d9szap94 178 15 genetic genetic JJ cord-014901-d9szap94 178 16 engineering engineering NN cord-014901-d9szap94 178 17 manipulations manipulation NNS cord-014901-d9szap94 178 18 . . . cord-014901-d9szap94 179 1 Known know VBN cord-014901-d9szap94 179 2 examples example NNS cord-014901-d9szap94 179 3 of of IN cord-014901-d9szap94 179 4 protein protein NN cord-014901-d9szap94 179 5 antigen antigen NN cord-014901-d9szap94 179 6 production production NN cord-014901-d9szap94 179 7 in in IN cord-014901-d9szap94 179 8 chloroplasts chloroplast NNS cord-014901-d9szap94 179 9 of of IN cord-014901-d9szap94 179 10 C. C. NNP cord-014901-d9szap94 179 11 reinhardtii reinhardtii NNP cord-014901-d9szap94 179 12 are be VBP cord-014901-d9szap94 179 13 B b NN cord-014901-d9szap94 179 14 subunit subunit NN cord-014901-d9szap94 179 15 of of IN cord-014901-d9szap94 179 16 cholera cholera NN cord-014901-d9szap94 179 17 toxin toxin NN cord-014901-d9szap94 179 18 fused fuse VBN cord-014901-d9szap94 179 19 with with IN cord-014901-d9szap94 179 20 the the DT cord-014901-d9szap94 179 21 coat coat NN cord-014901-d9szap94 179 22 protein protein NN cord-014901-d9szap94 179 23 of of IN cord-014901-d9szap94 179 24 foot foot NN cord-014901-d9szap94 179 25 and and CC cord-014901-d9szap94 179 26 mouth mouth NN cord-014901-d9szap94 179 27 disease disease NN cord-014901-d9szap94 179 28 virus virus NN cord-014901-d9szap94 179 29 [ [ -LRB- cord-014901-d9szap94 179 30 47 47 CD cord-014901-d9szap94 179 31 ] ] -RRB- cord-014901-d9szap94 179 32 or or CC cord-014901-d9szap94 179 33 with with IN cord-014901-d9szap94 179 34 D2 d2 NN cord-014901-d9szap94 179 35 fibronectin fibronectin NN cord-014901-d9szap94 179 36 binding bind VBG cord-014901-d9szap94 179 37 domain domain NN cord-014901-d9szap94 179 38 of of IN cord-014901-d9szap94 179 39 Staphylococ Staphylococ NNP cord-014901-d9szap94 179 40 cus cus NN cord-014901-d9szap94 179 41 aureus aureus NN cord-014901-d9szap94 179 42 [ [ -LRB- cord-014901-d9szap94 179 43 48 48 CD cord-014901-d9szap94 179 44 ] ] -RRB- cord-014901-d9szap94 179 45 , , , cord-014901-d9szap94 179 46 as as RB cord-014901-d9szap94 179 47 well well RB cord-014901-d9szap94 179 48 as as IN cord-014901-d9szap94 179 49 protein protein NN cord-014901-d9szap94 179 50 28 28 CD cord-014901-d9szap94 179 51 virus virus NN cord-014901-d9szap94 179 52 cryptokary cryptokary NNP cord-014901-d9szap94 179 53 osis osis NNP cord-014901-d9szap94 179 54 ( ( -LRB- cord-014901-d9szap94 179 55 shrimp shrimp NNP cord-014901-d9szap94 179 56 disease disease NNP cord-014901-d9szap94 179 57 ) ) -RRB- cord-014901-d9szap94 179 58 [ [ -LRB- cord-014901-d9szap94 179 59 49 49 CD cord-014901-d9szap94 179 60 ] ] -RRB- cord-014901-d9szap94 179 61 and and CC cord-014901-d9szap94 179 62 E2 E2 NNP cord-014901-d9szap94 179 63 protein protein NN cord-014901-d9szap94 179 64 of of IN cord-014901-d9szap94 179 65 swine swine NN cord-014901-d9szap94 179 66 fever fever NN cord-014901-d9szap94 179 67 [ [ -LRB- cord-014901-d9szap94 179 68 50 50 CD cord-014901-d9szap94 179 69 ] ] -RRB- cord-014901-d9szap94 179 70 . . . cord-014901-d9szap94 180 1 The the DT cord-014901-d9szap94 180 2 green green JJ cord-014901-d9szap94 180 3 moss moss NN cord-014901-d9szap94 180 4 Physcomitrella Physcomitrella NNP cord-014901-d9szap94 180 5 patens paten NNS cord-014901-d9szap94 180 6 is be VBZ cord-014901-d9szap94 180 7 the the DT cord-014901-d9szap94 180 8 only only JJ cord-014901-d9szap94 180 9 representative representative NN cord-014901-d9szap94 180 10 of of IN cord-014901-d9szap94 180 11 bryophytes bryophyte NNS cord-014901-d9szap94 180 12 , , , cord-014901-d9szap94 180 13 the the DT cord-014901-d9szap94 180 14 genome genome NN cord-014901-d9szap94 180 15 of of IN cord-014901-d9szap94 180 16 which which WDT cord-014901-d9szap94 180 17 is be VBZ cord-014901-d9szap94 180 18 currently currently RB cord-014901-d9szap94 180 19 completely completely RB cord-014901-d9szap94 180 20 sequenced sequence VBN cord-014901-d9szap94 180 21 and and CC cord-014901-d9szap94 180 22 approaches approach NNS cord-014901-d9szap94 180 23 to to IN cord-014901-d9szap94 180 24 its -PRON- PRP$ cord-014901-d9szap94 180 25 transformation transformation NN cord-014901-d9szap94 180 26 are be VBP cord-014901-d9szap94 180 27 developed develop VBN cord-014901-d9szap94 180 28 . . . cord-014901-d9szap94 181 1 The the DT cord-014901-d9szap94 181 2 peculiarity peculiarity NN cord-014901-d9szap94 181 3 of of IN cord-014901-d9szap94 181 4 this this DT cord-014901-d9szap94 181 5 moss moss NN cord-014901-d9szap94 181 6 is be VBZ cord-014901-d9szap94 181 7 that that IN cord-014901-d9szap94 181 8 at at IN cord-014901-d9szap94 181 9 the the DT cord-014901-d9szap94 181 10 stage stage NN cord-014901-d9szap94 181 11 of of IN cord-014901-d9szap94 181 12 the the DT cord-014901-d9szap94 181 13 haploid haploid JJ cord-014901-d9szap94 181 14 juvenile juvenile JJ cord-014901-d9szap94 181 15 game game NN cord-014901-d9szap94 181 16 tophyte tophyte NNP cord-014901-d9szap94 181 17 ( ( -LRB- cord-014901-d9szap94 181 18 protonema protonema NNP cord-014901-d9szap94 181 19 ) ) -RRB- cord-014901-d9szap94 182 1 this this DT cord-014901-d9szap94 182 2 moss moss NN cord-014901-d9szap94 182 3 is be VBZ cord-014901-d9szap94 182 4 morphologically morphologically RB cord-014901-d9szap94 182 5 similar similar JJ cord-014901-d9szap94 182 6 to to IN cord-014901-d9szap94 182 7 filamentous filamentous JJ cord-014901-d9szap94 182 8 algae algae NN cord-014901-d9szap94 182 9 and and CC cord-014901-d9szap94 182 10 easily easily RB cord-014901-d9szap94 182 11 enough enough JJ cord-014901-d9szap94 182 12 culti culti NNP cord-014901-d9szap94 182 13 vated vate VBN cord-014901-d9szap94 182 14 in in IN cord-014901-d9szap94 182 15 a a DT cord-014901-d9szap94 182 16 bioreactor bioreactor NN cord-014901-d9szap94 182 17 [ [ -LRB- cord-014901-d9szap94 182 18 25 25 CD cord-014901-d9szap94 182 19 , , , cord-014901-d9szap94 182 20 42 42 CD cord-014901-d9szap94 182 21 ] ] -RRB- cord-014901-d9szap94 182 22 . . . cord-014901-d9szap94 183 1 Under under IN cord-014901-d9szap94 183 2 certain certain JJ cord-014901-d9szap94 183 3 culture culture NN cord-014901-d9szap94 183 4 conditions condition NNS cord-014901-d9szap94 183 5 the the DT cord-014901-d9szap94 183 6 moss moss NN cord-014901-d9szap94 183 7 can can MD cord-014901-d9szap94 183 8 be be VB cord-014901-d9szap94 183 9 in in IN cord-014901-d9szap94 183 10 the the DT cord-014901-d9szap94 183 11 stage stage NN cord-014901-d9szap94 183 12 of of IN cord-014901-d9szap94 183 13 protonema protonema NNP cord-014901-d9szap94 183 14 indefinitely indefinitely RB cord-014901-d9szap94 183 15 long long RB cord-014901-d9szap94 183 16 . . . cord-014901-d9szap94 184 1 The the DT cord-014901-d9szap94 184 2 attractiveness attractiveness NN cord-014901-d9szap94 184 3 of of IN cord-014901-d9szap94 184 4 this this DT cord-014901-d9szap94 184 5 moss moss NN cord-014901-d9szap94 184 6 spe spe NNP cord-014901-d9szap94 184 7 cies cie NNS cord-014901-d9szap94 184 8 as as IN cord-014901-d9szap94 184 9 the the DT cord-014901-d9szap94 184 10 system system NN cord-014901-d9szap94 184 11 for for IN cord-014901-d9szap94 184 12 recombinant recombinant JJ cord-014901-d9szap94 184 13 protein protein NN cord-014901-d9szap94 184 14 expression expression NN cord-014901-d9szap94 184 15 is be VBZ cord-014901-d9szap94 184 16 that that IN cord-014901-d9szap94 184 17 , , , cord-014901-d9szap94 184 18 as as IN cord-014901-d9szap94 184 19 distinct distinct JJ cord-014901-d9szap94 184 20 from from IN cord-014901-d9szap94 184 21 plants plant NNS cord-014901-d9szap94 184 22 , , , cord-014901-d9szap94 184 23 fragments fragment NNS cord-014901-d9szap94 184 24 of of IN cord-014901-d9szap94 184 25 foreign foreign JJ cord-014901-d9szap94 184 26 DNA dna NN cord-014901-d9szap94 184 27 can can MD cord-014901-d9szap94 184 28 be be VB cord-014901-d9szap94 184 29 integrated integrate VBN cord-014901-d9szap94 184 30 in in IN cord-014901-d9szap94 184 31 its -PRON- PRP$ cord-014901-d9szap94 184 32 genome genome NN cord-014901-d9szap94 184 33 through through IN cord-014901-d9szap94 184 34 homo homo JJ cord-014901-d9szap94 184 35 logical logical JJ cord-014901-d9szap94 184 36 recombination recombination NN cord-014901-d9szap94 184 37 , , , cord-014901-d9szap94 184 38 which which WDT cord-014901-d9szap94 184 39 reduces reduce VBZ cord-014901-d9szap94 184 40 substantially substantially RB cord-014901-d9szap94 184 41 a a DT cord-014901-d9szap94 184 42 possibility possibility NN cord-014901-d9szap94 184 43 of of IN cord-014901-d9szap94 184 44 transferred transfer VBN cord-014901-d9szap94 184 45 gene gene NN cord-014901-d9szap94 184 46 inactivation inactivation NN cord-014901-d9szap94 184 47 . . . cord-014901-d9szap94 185 1 The the DT cord-014901-d9szap94 185 2 P. P. NNP cord-014901-d9szap94 185 3 pat pat NNP cord-014901-d9szap94 185 4 ens ens NNP cord-014901-d9szap94 185 5 cells cell NNS cord-014901-d9szap94 185 6 are be VBP cord-014901-d9szap94 185 7 capable capable JJ cord-014901-d9szap94 185 8 of of IN cord-014901-d9szap94 185 9 postranslational postranslational JJ cord-014901-d9szap94 185 10 modification modification NN cord-014901-d9szap94 185 11 of of IN cord-014901-d9szap94 185 12 proteins protein NNS cord-014901-d9szap94 185 13 of of IN cord-014901-d9szap94 185 14 eukaryotic eukaryotic JJ cord-014901-d9szap94 185 15 origin origin NN cord-014901-d9szap94 185 16 . . . cord-014901-d9szap94 186 1 Since since IN cord-014901-d9szap94 186 2 at at IN cord-014901-d9szap94 186 3 the the DT cord-014901-d9szap94 186 4 step step NN cord-014901-d9szap94 186 5 of of IN cord-014901-d9szap94 186 6 gametophyte gametophyte NN cord-014901-d9szap94 186 7 the the DT cord-014901-d9szap94 186 8 moss moss NN cord-014901-d9szap94 186 9 has have VBZ cord-014901-d9szap94 186 10 the the DT cord-014901-d9szap94 186 11 haploid haploid JJ cord-014901-d9szap94 186 12 number number NN cord-014901-d9szap94 186 13 of of IN cord-014901-d9szap94 186 14 chromosomes chromosome NNS cord-014901-d9szap94 186 15 , , , cord-014901-d9szap94 186 16 it -PRON- PRP cord-014901-d9szap94 186 17 becomes become VBZ cord-014901-d9szap94 186 18 possible possible JJ cord-014901-d9szap94 186 19 to to TO cord-014901-d9szap94 186 20 modify modify VB cord-014901-d9szap94 186 21 the the DT cord-014901-d9szap94 186 22 func func NN cord-014901-d9szap94 186 23 tioning tioning NN cord-014901-d9szap94 186 24 of of IN cord-014901-d9szap94 186 25 individual individual JJ cord-014901-d9szap94 186 26 genes gene NNS cord-014901-d9szap94 186 27 , , , cord-014901-d9szap94 186 28 in in IN cord-014901-d9szap94 186 29 particular particular JJ cord-014901-d9szap94 186 30 the the DT cord-014901-d9szap94 186 31 moss moss NN cord-014901-d9szap94 186 32 lines line NNS cord-014901-d9szap94 186 33 conducting conduct VBG cord-014901-d9szap94 186 34 glycosylation glycosylation NN cord-014901-d9szap94 186 35 of of IN cord-014901-d9szap94 186 36 recombinant recombinant JJ cord-014901-d9szap94 186 37 proteins protein NNS cord-014901-d9szap94 186 38 as as IN cord-014901-d9szap94 186 39 in in IN cord-014901-d9szap94 186 40 mammalian mammalian JJ cord-014901-d9szap94 186 41 cell cell NN cord-014901-d9szap94 186 42 type type NN cord-014901-d9szap94 186 43 were be VBD cord-014901-d9szap94 186 44 obtained obtain VBN cord-014901-d9szap94 186 45 [ [ -LRB- cord-014901-d9szap94 186 46 25 25 CD cord-014901-d9szap94 186 47 ] ] -RRB- cord-014901-d9szap94 186 48 . . . cord-014901-d9szap94 187 1 The the DT cord-014901-d9szap94 187 2 firm firm JJ cord-014901-d9szap94 187 3 Grenovation Grenovation NNP cord-014901-d9szap94 187 4 ( ( -LRB- cord-014901-d9szap94 187 5 Germany Germany NNP cord-014901-d9szap94 187 6 ) ) -RRB- cord-014901-d9szap94 187 7 is be VBZ cord-014901-d9szap94 187 8 developing develop VBG cord-014901-d9szap94 187 9 the the DT cord-014901-d9szap94 187 10 technology technology NN cord-014901-d9szap94 187 11 of of IN cord-014901-d9szap94 187 12 biopharmaceutical biopharmaceutical JJ cord-014901-d9szap94 187 13 protein protein NN cord-014901-d9szap94 187 14 production production NN cord-014901-d9szap94 187 15 on on IN cord-014901-d9szap94 187 16 the the DT cord-014901-d9szap94 187 17 basis basis NN cord-014901-d9szap94 187 18 of of IN cord-014901-d9szap94 187 19 P. P. NNP cord-014901-d9szap94 187 20 patens paten NNS cord-014901-d9szap94 187 21 in in IN cord-014901-d9szap94 187 22 bioreactors bioreactor NNS cord-014901-d9szap94 187 23 . . . cord-014901-d9szap94 188 1 Expression expression NN cord-014901-d9szap94 188 2 system system NN cord-014901-d9szap94 188 3 for for IN cord-014901-d9szap94 188 4 pro pro JJ cord-014901-d9szap94 188 5 ducing duce VBG cord-014901-d9szap94 188 6 biopharmaceuticals biopharmaceutical NNS cord-014901-d9szap94 188 7 based base VBN cord-014901-d9szap94 188 8 on on IN cord-014901-d9szap94 188 9 the the DT cord-014901-d9szap94 188 10 green green JJ cord-014901-d9szap94 188 11 moss moss NN cord-014901-d9szap94 188 12 is be VBZ cord-014901-d9szap94 188 13 not not RB cord-014901-d9szap94 188 14 the the DT cord-014901-d9szap94 188 15 part part NN cord-014901-d9szap94 188 16 of of IN cord-014901-d9szap94 188 17 the the DT cord-014901-d9szap94 188 18 food food NN cord-014901-d9szap94 188 19 chain chain NN cord-014901-d9szap94 188 20 and and CC cord-014901-d9szap94 188 21 is be VBZ cord-014901-d9szap94 188 22 characterized characterize VBN cord-014901-d9szap94 188 23 by by IN cord-014901-d9szap94 188 24 a a DT cord-014901-d9szap94 188 25 high high JJ cord-014901-d9szap94 188 26 degree degree NN cord-014901-d9szap94 188 27 of of IN cord-014901-d9szap94 188 28 biosafety biosafety NN cord-014901-d9szap94 188 29 . . . cord-014901-d9szap94 189 1 As as IN cord-014901-d9szap94 189 2 distinct distinct JJ cord-014901-d9szap94 189 3 from from IN cord-014901-d9szap94 189 4 above above IN cord-014901-d9szap94 189 5 described describe VBN cord-014901-d9szap94 189 6 systems system NNS cord-014901-d9szap94 189 7 based base VBN cord-014901-d9szap94 189 8 on on IN cord-014901-d9szap94 189 9 the the DT cord-014901-d9szap94 189 10 stable stable JJ cord-014901-d9szap94 189 11 expression expression NN cord-014901-d9szap94 189 12 of of IN cord-014901-d9szap94 189 13 foreign foreign JJ cord-014901-d9szap94 189 14 genes gene NNS cord-014901-d9szap94 189 15 integrated integrate VBN cord-014901-d9szap94 189 16 into into IN cord-014901-d9szap94 189 17 nuclear nuclear JJ cord-014901-d9szap94 189 18 or or CC cord-014901-d9szap94 189 19 chloroplast chloroplast NN cord-014901-d9szap94 189 20 genomes genome NNS cord-014901-d9szap94 189 21 , , , cord-014901-d9szap94 189 22 during during IN cord-014901-d9szap94 189 23 transient transient JJ cord-014901-d9szap94 189 24 expression expression NN cord-014901-d9szap94 189 25 target target NN cord-014901-d9szap94 189 26 proteins protein NNS cord-014901-d9szap94 189 27 are be VBP cord-014901-d9szap94 189 28 synthesized synthesize VBN cord-014901-d9szap94 189 29 in in IN cord-014901-d9szap94 189 30 the the DT cord-014901-d9szap94 189 31 plant plant NN cord-014901-d9szap94 189 32 cell cell NN cord-014901-d9szap94 189 33 during during IN cord-014901-d9szap94 189 34 relatively relatively RB cord-014901-d9szap94 189 35 short short JJ cord-014901-d9szap94 189 36 time time NN cord-014901-d9szap94 189 37 ( ( -LRB- cord-014901-d9szap94 189 38 several several JJ cord-014901-d9szap94 189 39 days day NNS cord-014901-d9szap94 189 40 ) ) -RRB- cord-014901-d9szap94 189 41 without without IN cord-014901-d9szap94 189 42 insertion insertion NN cord-014901-d9szap94 189 43 into into IN cord-014901-d9szap94 189 44 the the DT cord-014901-d9szap94 189 45 plant plant NN cord-014901-d9szap94 189 46 genome genome NN cord-014901-d9szap94 189 47 . . . cord-014901-d9szap94 190 1 At at IN cord-014901-d9szap94 190 2 present present NN cord-014901-d9szap94 190 3 , , , cord-014901-d9szap94 190 4 the the DT cord-014901-d9szap94 190 5 fol fol NN cord-014901-d9szap94 190 6 lowing lowing NN cord-014901-d9szap94 190 7 approaches approach NNS cord-014901-d9szap94 190 8 are be VBP cord-014901-d9szap94 190 9 used use VBN cord-014901-d9szap94 190 10 for for IN cord-014901-d9szap94 190 11 transient transient JJ cord-014901-d9szap94 190 12 gene gene NN cord-014901-d9szap94 190 13 expres expres NNP cord-014901-d9szap94 190 14 sion sion NNP cord-014901-d9szap94 190 15 in in IN cord-014901-d9szap94 190 16 plants plant NNS cord-014901-d9szap94 190 17 : : : cord-014901-d9szap94 190 18 gene gene NN cord-014901-d9szap94 190 19 delivery delivery NN cord-014901-d9szap94 190 20 with with IN cord-014901-d9szap94 190 21 the the DT cord-014901-d9szap94 190 22 help help NN cord-014901-d9szap94 190 23 of of IN cord-014901-d9szap94 190 24 agrobac agrobac NNP cord-014901-d9szap94 190 25 terium terium NNP cord-014901-d9szap94 190 26 , , , cord-014901-d9szap94 190 27 the the DT cord-014901-d9szap94 190 28 use use NN cord-014901-d9szap94 190 29 of of IN cord-014901-d9szap94 190 30 plant plant NN cord-014901-d9szap94 190 31 virus virus NN cord-014901-d9szap94 190 32 vectors vector NNS cord-014901-d9szap94 190 33 , , , cord-014901-d9szap94 190 34 and and CC cord-014901-d9szap94 190 35 magnifec magnifec NNP cord-014901-d9szap94 190 36 tion tion NNP cord-014901-d9szap94 190 37 [ [ -LRB- cord-014901-d9szap94 190 38 51 51 CD cord-014901-d9szap94 190 39 ] ] -RRB- cord-014901-d9szap94 190 40 [ [ -LRB- cord-014901-d9szap94 190 41 52 52 CD cord-014901-d9szap94 190 42 ] ] -RRB- cord-014901-d9szap94 190 43 [ [ -LRB- cord-014901-d9szap94 190 44 53 53 CD cord-014901-d9szap94 190 45 ] ] -RRB- cord-014901-d9szap94 190 46 . . . cord-014901-d9szap94 191 1 Tobacco tobacco NN cord-014901-d9szap94 191 2 mosaic mosaic JJ cord-014901-d9szap94 191 3 virus virus NN cord-014901-d9szap94 191 4 ( ( -LRB- cord-014901-d9szap94 191 5 TMV TMV NNP cord-014901-d9szap94 191 6 ) ) -RRB- cord-014901-d9szap94 191 7 , , , cord-014901-d9szap94 191 8 potato potato NN cord-014901-d9szap94 191 9 X x NN cord-014901-d9szap94 191 10 virus virus NN cord-014901-d9szap94 191 11 , , , cord-014901-d9szap94 191 12 alfalfa alfalfa NN cord-014901-d9szap94 191 13 mosaic mosaic NN cord-014901-d9szap94 191 14 virus virus NN cord-014901-d9szap94 191 15 , , , cord-014901-d9szap94 191 16 and and CC cord-014901-d9szap94 191 17 cowpea cowpea NNP cord-014901-d9szap94 191 18 mosaic mosaic NN cord-014901-d9szap94 191 19 virus virus NN cord-014901-d9szap94 191 20 are be VBP cord-014901-d9szap94 191 21 used use VBN cord-014901-d9szap94 191 22 as as IN cord-014901-d9szap94 191 23 virus virus NN cord-014901-d9szap94 191 24 vectors vector NNS cord-014901-d9szap94 191 25 [ [ -LRB- cord-014901-d9szap94 191 26 52 52 CD cord-014901-d9szap94 191 27 ] ] -RRB- cord-014901-d9szap94 191 28 . . . cord-014901-d9szap94 192 1 The the DT cord-014901-d9szap94 192 2 availability availability NN cord-014901-d9szap94 192 3 of of IN cord-014901-d9szap94 192 4 infec infec IN cord-014901-d9szap94 192 5 tious tious NNP cord-014901-d9szap94 192 6 cDNA cDNA NNP cord-014901-d9szap94 192 7 clones clone NNS cord-014901-d9szap94 192 8 , , , cord-014901-d9szap94 192 9 the the DT cord-014901-d9szap94 192 10 small small JJ cord-014901-d9szap94 192 11 size size NN cord-014901-d9szap94 192 12 of of IN cord-014901-d9szap94 192 13 the the DT cord-014901-d9szap94 192 14 viral viral JJ cord-014901-d9szap94 192 15 genomes genome NNS cord-014901-d9szap94 192 16 , , , cord-014901-d9szap94 192 17 the the DT cord-014901-d9szap94 192 18 short short JJ cord-014901-d9szap94 192 19 time time NN cord-014901-d9szap94 192 20 required require VBN cord-014901-d9szap94 192 21 for for IN cord-014901-d9szap94 192 22 the the DT cord-014901-d9szap94 192 23 expression expression NN cord-014901-d9szap94 192 24 of of IN cord-014901-d9szap94 192 25 a a DT cord-014901-d9szap94 192 26 target target NN cord-014901-d9szap94 192 27 gene gene NN cord-014901-d9szap94 192 28 , , , cord-014901-d9szap94 192 29 and and CC cord-014901-d9szap94 192 30 a a DT cord-014901-d9szap94 192 31 high high JJ cord-014901-d9szap94 192 32 level level NN cord-014901-d9szap94 192 33 of of IN cord-014901-d9szap94 192 34 expression expression NN cord-014901-d9szap94 192 35 provides provide VBZ cord-014901-d9szap94 192 36 for for IN cord-014901-d9szap94 192 37 a a DT cord-014901-d9szap94 192 38 high high JJ cord-014901-d9szap94 192 39 attraction attraction NN cord-014901-d9szap94 192 40 of of IN cord-014901-d9szap94 192 41 this this DT cord-014901-d9szap94 192 42 system system NN cord-014901-d9szap94 192 43 . . . cord-014901-d9szap94 193 1 The the DT cord-014901-d9szap94 193 2 rapid rapid JJ cord-014901-d9szap94 193 3 development development NN cord-014901-d9szap94 193 4 of of IN cord-014901-d9szap94 193 5 this this DT cord-014901-d9szap94 193 6 expression expression NN cord-014901-d9szap94 193 7 system system NN cord-014901-d9szap94 193 8 led lead VBD cord-014901-d9szap94 193 9 to to IN cord-014901-d9szap94 193 10 substantial substantial JJ cord-014901-d9szap94 193 11 modifications modification NNS cord-014901-d9szap94 193 12 of of IN cord-014901-d9szap94 193 13 the the DT cord-014901-d9szap94 193 14 first first JJ cord-014901-d9szap94 193 15 gene gene NN cord-014901-d9szap94 193 16 inser inser NNP cord-014901-d9szap94 193 17 tion tion NN cord-014901-d9szap94 193 18 vectors vector NNS cord-014901-d9szap94 193 19 or or CC cord-014901-d9szap94 193 20 full full JJ cord-014901-d9szap94 193 21 virus virus NN cord-014901-d9szap94 193 22 vectors vector NNS cord-014901-d9szap94 193 23 , , , cord-014901-d9szap94 193 24 which which WDT cord-014901-d9szap94 193 25 is be VBZ cord-014901-d9szap94 193 26 a a DT cord-014901-d9szap94 193 27 recombi recombi NNP cord-014901-d9szap94 193 28 nant nant NNP cord-014901-d9szap94 193 29 virus virus NN cord-014901-d9szap94 193 30 that that WDT cord-014901-d9szap94 193 31 behaves behave VBZ cord-014901-d9szap94 193 32 as as IN cord-014901-d9szap94 193 33 wild wild JJ cord-014901-d9szap94 193 34 type type NN cord-014901-d9szap94 193 35 virus virus NN cord-014901-d9szap94 193 36 but but CC cord-014901-d9szap94 193 37 is be VBZ cord-014901-d9szap94 193 38 capable capable JJ cord-014901-d9szap94 193 39 of of IN cord-014901-d9szap94 193 40 expressing express VBG cord-014901-d9szap94 193 41 additional additional JJ cord-014901-d9szap94 193 42 genes gene NNS cord-014901-d9szap94 193 43 . . . cord-014901-d9szap94 194 1 The the DT cord-014901-d9szap94 194 2 next next JJ cord-014901-d9szap94 194 3 step step NN cord-014901-d9szap94 194 4 was be VBD cord-014901-d9szap94 194 5 the the DT cord-014901-d9szap94 194 6 creation creation NN cord-014901-d9szap94 194 7 of of IN cord-014901-d9szap94 194 8 " " `` cord-014901-d9szap94 194 9 disarmed disarm VBN cord-014901-d9szap94 194 10 vectors vector NNS cord-014901-d9szap94 194 11 " " '' cord-014901-d9szap94 194 12 ( ( -LRB- cord-014901-d9szap94 194 13 deconstructed deconstruct VBN cord-014901-d9szap94 194 14 vec vec NN cord-014901-d9szap94 194 15 tors tor NNS cord-014901-d9szap94 194 16 ) ) -RRB- cord-014901-d9szap94 194 17 lacking lack VBG cord-014901-d9szap94 194 18 a a DT cord-014901-d9szap94 194 19 number number NN cord-014901-d9szap94 194 20 of of IN cord-014901-d9szap94 194 21 original original JJ cord-014901-d9szap94 194 22 virus virus NN cord-014901-d9szap94 194 23 genes gene NNS cord-014901-d9szap94 194 24 , , , cord-014901-d9szap94 194 25 and and CC cord-014901-d9szap94 194 26 gene gene NN cord-014901-d9szap94 194 27 replacement replacement NN cord-014901-d9szap94 194 28 vectors vector NNS cord-014901-d9szap94 194 29 , , , cord-014901-d9szap94 194 30 in in IN cord-014901-d9szap94 194 31 which which WDT cord-014901-d9szap94 194 32 a a DT cord-014901-d9szap94 194 33 portion portion NN cord-014901-d9szap94 194 34 of of IN cord-014901-d9szap94 194 35 the the DT cord-014901-d9szap94 194 36 viral viral JJ cord-014901-d9szap94 194 37 genes gene NNS cord-014901-d9szap94 194 38 is be VBZ cord-014901-d9szap94 194 39 replaced replace VBN cord-014901-d9szap94 194 40 by by IN cord-014901-d9szap94 194 41 alien alien JJ cord-014901-d9szap94 194 42 genes gene NNS cord-014901-d9szap94 194 43 [ [ -LRB- cord-014901-d9szap94 194 44 51 51 CD cord-014901-d9szap94 194 45 ] ] -RRB- cord-014901-d9szap94 194 46 . . . cord-014901-d9szap94 195 1 Viral viral JJ cord-014901-d9szap94 195 2 vectors vector NNS cord-014901-d9szap94 195 3 have have VBP cord-014901-d9szap94 195 4 several several JJ cord-014901-d9szap94 195 5 substantial substantial JJ cord-014901-d9szap94 195 6 disadvantages disadvantage NNS cord-014901-d9szap94 195 7 : : : cord-014901-d9szap94 195 8 a a DT cord-014901-d9szap94 195 9 tendency tendency NN cord-014901-d9szap94 195 10 to to IN cord-014901-d9szap94 195 11 the the DT cord-014901-d9szap94 195 12 loss loss NN cord-014901-d9szap94 195 13 of of IN cord-014901-d9szap94 195 14 foreign foreign JJ cord-014901-d9szap94 195 15 insertion insertion NN cord-014901-d9szap94 195 16 in in IN cord-014901-d9szap94 195 17 the the DT cord-014901-d9szap94 195 18 process process NN cord-014901-d9szap94 195 19 of of IN cord-014901-d9szap94 195 20 virus virus NN cord-014901-d9szap94 195 21 spreading spread VBG cord-014901-d9szap94 195 22 over over IN cord-014901-d9szap94 195 23 the the DT cord-014901-d9szap94 195 24 plant plant NN cord-014901-d9szap94 195 25 and and CC cord-014901-d9szap94 195 26 a a DT cord-014901-d9szap94 195 27 potential potential JJ cord-014901-d9szap94 195 28 risk risk NN cord-014901-d9szap94 195 29 for for IN cord-014901-d9szap94 195 30 envi envi NNP cord-014901-d9szap94 195 31 ronment ronment NNP cord-014901-d9szap94 195 32 related relate VBN cord-014901-d9szap94 195 33 to to IN cord-014901-d9szap94 195 34 the the DT cord-014901-d9szap94 195 35 presence presence NN cord-014901-d9szap94 195 36 of of IN cord-014901-d9szap94 195 37 infectious infectious JJ cord-014901-d9szap94 195 38 recombi recombi NNP cord-014901-d9szap94 195 39 nant nant JJ cord-014901-d9szap94 195 40 viral viral JJ cord-014901-d9szap94 195 41 particles particle NNS cord-014901-d9szap94 195 42 . . . cord-014901-d9szap94 196 1 Launch launch NN cord-014901-d9szap94 196 2 vectors vector NNS cord-014901-d9szap94 196 3 represent represent VBP cord-014901-d9szap94 196 4 an an DT cord-014901-d9szap94 196 5 alternative alternative NN cord-014901-d9szap94 196 6 to to IN cord-014901-d9szap94 196 7 recom recom VB cord-014901-d9szap94 196 8 binant binant JJ cord-014901-d9szap94 196 9 plant plant NN cord-014901-d9szap94 196 10 viruses virus NNS cord-014901-d9szap94 196 11 ; ; : cord-014901-d9szap94 196 12 cDNA cdna NN cord-014901-d9szap94 196 13 of of IN cord-014901-d9szap94 196 14 these these DT cord-014901-d9szap94 196 15 viruses virus NNS cord-014901-d9szap94 196 16 is be VBZ cord-014901-d9szap94 196 17 deliv deliv NNP cord-014901-d9szap94 196 18 ered ered JJ cord-014901-d9szap94 196 19 to to IN cord-014901-d9szap94 196 20 plants plant NNS cord-014901-d9szap94 196 21 within within IN cord-014901-d9szap94 196 22 T T NNP cord-014901-d9szap94 196 23 DNA DNA NNP cord-014901-d9szap94 196 24 region region NN cord-014901-d9szap94 196 25 of of IN cord-014901-d9szap94 196 26 agrobacterial agrobacterial JJ cord-014901-d9szap94 196 27 Ti Ti NNP cord-014901-d9szap94 196 28 plasmid plasmid NN cord-014901-d9szap94 196 29 . . . cord-014901-d9szap94 197 1 Firstly firstly RB cord-014901-d9szap94 197 2 , , , cord-014901-d9szap94 197 3 primary primary JJ cord-014901-d9szap94 197 4 transcription transcription NN cord-014901-d9szap94 197 5 of of IN cord-014901-d9szap94 197 6 T T NNP cord-014901-d9szap94 197 7 DNA dna NN cord-014901-d9szap94 197 8 occurs occur VBZ cord-014901-d9szap94 197 9 in in IN cord-014901-d9szap94 197 10 the the DT cord-014901-d9szap94 197 11 nucleus nucleus NN cord-014901-d9szap94 197 12 ; ; : cord-014901-d9szap94 197 13 then then RB cord-014901-d9szap94 197 14 viral viral JJ cord-014901-d9szap94 197 15 RNA RNA NNP cord-014901-d9szap94 197 16 is be VBZ cord-014901-d9szap94 197 17 released release VBN cord-014901-d9szap94 197 18 into into IN cord-014901-d9szap94 197 19 the the DT cord-014901-d9szap94 197 20 cytoplasm cytoplasm NN cord-014901-d9szap94 197 21 , , , cord-014901-d9szap94 197 22 where where WRB cord-014901-d9szap94 197 23 its -PRON- PRP$ cord-014901-d9szap94 197 24 further further JJ cord-014901-d9szap94 197 25 amplification amplification NN cord-014901-d9szap94 197 26 , , , cord-014901-d9szap94 197 27 trans trans NNP cord-014901-d9szap94 197 28 lation lation NNP cord-014901-d9szap94 197 29 , , , cord-014901-d9szap94 197 30 and and CC cord-014901-d9szap94 197 31 protein protein NN cord-014901-d9szap94 197 32 synthesis synthesis NN cord-014901-d9szap94 197 33 occur occur VBP cord-014901-d9szap94 197 34 [ [ -LRB- cord-014901-d9szap94 197 35 52 52 CD cord-014901-d9szap94 197 36 ] ] -RRB- cord-014901-d9szap94 197 37 . . . cord-014901-d9szap94 198 1 By by IN cord-014901-d9szap94 198 2 2005 2005 CD cord-014901-d9szap94 198 3 , , , cord-014901-d9szap94 198 4 the the DT cord-014901-d9szap94 198 5 system system NN cord-014901-d9szap94 198 6 of of IN cord-014901-d9szap94 198 7 agroinfiltration agroinfiltration NN cord-014901-d9szap94 198 8 based base VBN cord-014901-d9szap94 198 9 on on IN cord-014901-d9szap94 198 10 the the DT cord-014901-d9szap94 198 11 use use NN cord-014901-d9szap94 198 12 of of IN cord-014901-d9szap94 198 13 plant plant NN cord-014901-d9szap94 198 14 viruses virus NNS cord-014901-d9szap94 198 15 and and CC cord-014901-d9szap94 198 16 agrobacterial agrobacterial JJ cord-014901-d9szap94 198 17 binary binary JJ cord-014901-d9szap94 198 18 plasmids plasmid NNS cord-014901-d9szap94 198 19 was be VBD cord-014901-d9szap94 198 20 upgraded upgrade VBN cord-014901-d9szap94 198 21 and and CC cord-014901-d9szap94 198 22 named name VBN cord-014901-d9szap94 198 23 as as IN cord-014901-d9szap94 198 24 magnifection magnifection NNP cord-014901-d9szap94 198 25 [ [ -LRB- cord-014901-d9szap94 198 26 54 54 CD cord-014901-d9szap94 198 27 ] ] -RRB- cord-014901-d9szap94 198 28 . . . cord-014901-d9szap94 199 1 At at IN cord-014901-d9szap94 199 2 magnifection magnifection NN cord-014901-d9szap94 199 3 , , , cord-014901-d9szap94 199 4 multiple multiple JJ cord-014901-d9szap94 199 5 agrobacterial agrobacterial JJ cord-014901-d9szap94 199 6 lines line NNS cord-014901-d9szap94 199 7 carrying carry VBG cord-014901-d9szap94 199 8 different different JJ cord-014901-d9szap94 199 9 parts part NNS cord-014901-d9szap94 199 10 of of IN cord-014901-d9szap94 199 11 the the DT cord-014901-d9szap94 199 12 TMV TMV NNP cord-014901-d9szap94 199 13 genome genome NN cord-014901-d9szap94 199 14 are be VBP cord-014901-d9szap94 199 15 used use VBN cord-014901-d9szap94 199 16 simulta simulta NNP cord-014901-d9szap94 199 17 neously neously NNP cord-014901-d9szap94 199 18 . . . cord-014901-d9szap94 200 1 After after IN cord-014901-d9szap94 200 2 agrobacterial agrobacterial JJ cord-014901-d9szap94 200 3 transfer transfer NN cord-014901-d9szap94 200 4 into into IN cord-014901-d9szap94 200 5 the the DT cord-014901-d9szap94 200 6 plant plant NN cord-014901-d9szap94 200 7 cell cell NN cord-014901-d9szap94 200 8 nucleus nucleus NN cord-014901-d9szap94 200 9 , , , cord-014901-d9szap94 200 10 separate separate JJ cord-014901-d9szap94 200 11 parts part NNS cord-014901-d9szap94 200 12 of of IN cord-014901-d9szap94 200 13 viral viral JJ cord-014901-d9szap94 200 14 genome genome NN cord-014901-d9szap94 200 15 are be VBP cord-014901-d9szap94 200 16 assembled assemble VBN cord-014901-d9szap94 200 17 in in IN cord-014901-d9szap94 200 18 plants plant NNS cord-014901-d9szap94 200 19 in in IN cord-014901-d9szap94 200 20 the the DT cord-014901-d9szap94 200 21 completely completely RB cord-014901-d9szap94 200 22 functional functional JJ cord-014901-d9szap94 200 23 viral viral JJ cord-014901-d9szap94 200 24 replicon replicon NN cord-014901-d9szap94 200 25 [ [ -LRB- cord-014901-d9szap94 200 26 51 51 CD cord-014901-d9szap94 200 27 ] ] -RRB- cord-014901-d9szap94 200 28 . . . cord-014901-d9szap94 201 1 The the DT cord-014901-d9szap94 201 2 substantial substantial JJ cord-014901-d9szap94 201 3 modification modification NN cord-014901-d9szap94 201 4 of of IN cord-014901-d9szap94 201 5 the the DT cord-014901-d9szap94 201 6 viral viral JJ cord-014901-d9szap94 201 7 genome genome NN cord-014901-d9szap94 201 8 , , , cord-014901-d9szap94 201 9 including include VBG cord-014901-d9szap94 201 10 numerous numerous JJ cord-014901-d9szap94 201 11 point point NN cord-014901-d9szap94 201 12 mutations mutation NNS cord-014901-d9szap94 201 13 for for IN cord-014901-d9szap94 201 14 the the DT cord-014901-d9szap94 201 15 removal removal NN cord-014901-d9szap94 201 16 of of IN cord-014901-d9szap94 201 17 potential potential JJ cord-014901-d9szap94 201 18 sites site NNS cord-014901-d9szap94 201 19 of of IN cord-014901-d9szap94 201 20 splicing splicing NN cord-014901-d9szap94 201 21 , , , cord-014901-d9szap94 201 22 intron intron NN cord-014901-d9szap94 201 23 insertion insertion NN cord-014901-d9szap94 201 24 , , , cord-014901-d9szap94 201 25 and and CC cord-014901-d9szap94 201 26 the the DT cord-014901-d9szap94 201 27 removal removal NN cord-014901-d9szap94 201 28 of of IN cord-014901-d9szap94 201 29 the the DT cord-014901-d9szap94 201 30 gene gene NN cord-014901-d9szap94 201 31 encoding encoding NN cord-014901-d9szap94 201 32 envelope envelope NN cord-014901-d9szap94 201 33 proteins protein NNS cord-014901-d9szap94 201 34 , , , cord-014901-d9szap94 201 35 pro pro JJ cord-014901-d9szap94 201 36 vided vide VBN cord-014901-d9szap94 201 37 for for IN cord-014901-d9szap94 201 38 the the DT cord-014901-d9szap94 201 39 highly highly RB cord-014901-d9szap94 201 40 efficient efficient JJ cord-014901-d9szap94 201 41 system system NN cord-014901-d9szap94 201 42 capable capable JJ cord-014901-d9szap94 201 43 of of IN cord-014901-d9szap94 201 44 recom recom NNP cord-014901-d9szap94 201 45 binant binant NNP cord-014901-d9szap94 201 46 protein protein NNP cord-014901-d9szap94 201 47 synthesis synthesis NNP cord-014901-d9szap94 201 48 ( ( -LRB- cord-014901-d9szap94 201 49 up up IN cord-014901-d9szap94 201 50 to to TO cord-014901-d9szap94 201 51 5 5 CD cord-014901-d9szap94 201 52 g g NN cord-014901-d9szap94 201 53 / / SYM cord-014901-d9szap94 201 54 kg kg NN cord-014901-d9szap94 201 55 of of IN cord-014901-d9szap94 201 56 fresh fresh JJ cord-014901-d9szap94 201 57 tissue tissue NN cord-014901-d9szap94 201 58 ) ) -RRB- cord-014901-d9szap94 201 59 , , , cord-014901-d9szap94 201 60 which which WDT cord-014901-d9szap94 201 61 is be VBZ cord-014901-d9szap94 201 62 more more JJR cord-014901-d9szap94 201 63 than than IN cord-014901-d9szap94 201 64 50 50 CD cord-014901-d9szap94 201 65 % % NN cord-014901-d9szap94 201 66 of of IN cord-014901-d9szap94 201 67 TSP tsp NN cord-014901-d9szap94 201 68 [ [ -LRB- cord-014901-d9szap94 201 69 51 51 CD cord-014901-d9szap94 201 70 ] ] -RRB- cord-014901-d9szap94 201 71 . . . cord-014901-d9szap94 202 1 Among among IN cord-014901-d9szap94 202 2 disadvantages disadvantage NNS cord-014901-d9szap94 202 3 of of IN cord-014901-d9szap94 202 4 transient transient JJ cord-014901-d9szap94 202 5 system system NN cord-014901-d9szap94 202 6 is be VBZ cord-014901-d9szap94 202 7 a a DT cord-014901-d9szap94 202 8 necessity necessity NN cord-014901-d9szap94 202 9 for for IN cord-014901-d9szap94 202 10 recombinant recombinant JJ cord-014901-d9szap94 202 11 protein protein NN cord-014901-d9szap94 202 12 isolation isolation NN cord-014901-d9szap94 202 13 and and CC cord-014901-d9szap94 202 14 purifi purifi NNP cord-014901-d9szap94 202 15 cation cation NN cord-014901-d9szap94 202 16 immediately immediately RB cord-014901-d9szap94 202 17 after after IN cord-014901-d9szap94 202 18 its -PRON- PRP$ cord-014901-d9szap94 202 19 accumulation accumulation NN cord-014901-d9szap94 202 20 in in IN cord-014901-d9szap94 202 21 the the DT cord-014901-d9szap94 202 22 plant plant NN cord-014901-d9szap94 202 23 , , , cord-014901-d9szap94 202 24 because because IN cord-014901-d9szap94 202 25 , , , cord-014901-d9szap94 202 26 as as IN cord-014901-d9szap94 202 27 distinct distinct JJ cord-014901-d9szap94 202 28 from from IN cord-014901-d9szap94 202 29 seeds seed NNS cord-014901-d9szap94 202 30 and and CC cord-014901-d9szap94 202 31 fruits fruit NNS cord-014901-d9szap94 202 32 , , , cord-014901-d9szap94 202 33 plant plant NN cord-014901-d9szap94 202 34 leaves leave NNS cord-014901-d9szap94 202 35 and and CC cord-014901-d9szap94 202 36 stems stem VBZ cord-014901-d9szap94 202 37 can can MD cord-014901-d9szap94 202 38 not not RB cord-014901-d9szap94 202 39 be be VB cord-014901-d9szap94 202 40 stored store VBN cord-014901-d9szap94 202 41 for for IN cord-014901-d9szap94 202 42 a a DT cord-014901-d9szap94 202 43 long long JJ cord-014901-d9szap94 202 44 time time NN cord-014901-d9szap94 202 45 . . . cord-014901-d9szap94 203 1 The the DT cord-014901-d9szap94 203 2 systems system NNS cord-014901-d9szap94 203 3 of of IN cord-014901-d9szap94 203 4 transient transient JJ cord-014901-d9szap94 203 5 expression expression NN cord-014901-d9szap94 203 6 of of IN cord-014901-d9szap94 203 7 recombinant recombinant JJ cord-014901-d9szap94 203 8 proteins protein NNS cord-014901-d9szap94 203 9 are be VBP cord-014901-d9szap94 203 10 rather rather RB cord-014901-d9szap94 203 11 promising promise VBG cord-014901-d9szap94 203 12 in in IN cord-014901-d9szap94 203 13 the the DT cord-014901-d9szap94 203 14 cases case NNS cord-014901-d9szap94 203 15 when when WRB cord-014901-d9szap94 203 16 a a DT cord-014901-d9szap94 203 17 rapid rapid JJ cord-014901-d9szap94 203 18 production production NN cord-014901-d9szap94 203 19 of of IN cord-014901-d9szap94 203 20 a a DT cord-014901-d9szap94 203 21 small small JJ cord-014901-d9szap94 203 22 amount amount NN cord-014901-d9szap94 203 23 of of IN cord-014901-d9szap94 203 24 proteins protein NNS cord-014901-d9szap94 203 25 is be VBZ cord-014901-d9szap94 203 26 required require VBN cord-014901-d9szap94 203 27 . . . cord-014901-d9szap94 204 1 Experiments experiment NNS cord-014901-d9szap94 204 2 with with IN cord-014901-d9szap94 204 3 transient transient JJ cord-014901-d9szap94 204 4 expression expression NN cord-014901-d9szap94 204 5 in in IN cord-014901-d9szap94 204 6 plants plant NNS cord-014901-d9szap94 204 7 are be VBP cord-014901-d9szap94 204 8 held hold VBN cord-014901-d9szap94 204 9 indoors indoor NNS cord-014901-d9szap94 204 10 , , , cord-014901-d9szap94 204 11 which which WDT cord-014901-d9szap94 204 12 reduces reduce VBZ cord-014901-d9szap94 204 13 the the DT cord-014901-d9szap94 204 14 risks risk NNS cord-014901-d9szap94 204 15 associated associate VBN cord-014901-d9szap94 204 16 with with IN cord-014901-d9szap94 204 17 biosafety biosafety NN cord-014901-d9szap94 204 18 to to IN cord-014901-d9szap94 204 19 almost almost RB cord-014901-d9szap94 204 20 zero zero CD cord-014901-d9szap94 204 21 . . . cord-014901-d9szap94 205 1 Examples example NNS cord-014901-d9szap94 205 2 of of IN cord-014901-d9szap94 205 3 manufacturing manufacture VBG cord-014901-d9szap94 205 4 immunogenic immunogenic JJ cord-014901-d9szap94 205 5 proteins protein NNS cord-014901-d9szap94 205 6 for for IN cord-014901-d9szap94 205 7 veterinary veterinary JJ cord-014901-d9szap94 205 8 using use VBG cord-014901-d9szap94 205 9 transient transient JJ cord-014901-d9szap94 205 10 expression expression NN cord-014901-d9szap94 205 11 systems system NNS cord-014901-d9szap94 205 12 are be VBP cord-014901-d9szap94 205 13 presented present VBN cord-014901-d9szap94 205 14 in in IN cord-014901-d9szap94 205 15 Table Table NNP cord-014901-d9szap94 205 16 1 1 CD cord-014901-d9szap94 205 17 . . . cord-014901-d9szap94 206 1 The the DT cord-014901-d9szap94 206 2 sys sys NN cord-014901-d9szap94 206 3 tems tem NNS cord-014901-d9szap94 206 4 of of IN cord-014901-d9szap94 206 5 transient transient JJ cord-014901-d9szap94 206 6 expression expression NN cord-014901-d9szap94 206 7 for for IN cord-014901-d9szap94 206 8 the the DT cord-014901-d9szap94 206 9 production production NN cord-014901-d9szap94 206 10 of of IN cord-014901-d9szap94 206 11 recombinant recombinant JJ cord-014901-d9szap94 206 12 proteins protein NNS cord-014901-d9szap94 206 13 are be VBP cord-014901-d9szap94 206 14 described describe VBN cord-014901-d9szap94 206 15 in in IN cord-014901-d9szap94 206 16 more more JJR cord-014901-d9szap94 206 17 details detail NNS cord-014901-d9szap94 206 18 in in IN cord-014901-d9szap94 206 19 reviews review NNS cord-014901-d9szap94 206 20 [ [ -LRB- cord-014901-d9szap94 206 21 1 1 CD cord-014901-d9szap94 206 22 , , , cord-014901-d9szap94 206 23 41 41 CD cord-014901-d9szap94 206 24 , , , cord-014901-d9szap94 206 25 51 51 CD cord-014901-d9szap94 206 26 , , , cord-014901-d9szap94 206 27 52 52 CD cord-014901-d9szap94 206 28 , , , cord-014901-d9szap94 206 29 54 54 CD cord-014901-d9szap94 206 30 ] ] -RRB- cord-014901-d9szap94 206 31 . . . cord-014901-d9szap94 206 32 " " '' cord-014901-d9szap94 207 1 CANDIDATE candidate NN cord-014901-d9szap94 207 2 " " `` cord-014901-d9szap94 207 3 PLANT plant NN cord-014901-d9szap94 207 4 VACCINES vaccine NNS cord-014901-d9szap94 207 5 FOR for IN cord-014901-d9szap94 207 6 VETERINARY veterinary JJ cord-014901-d9szap94 207 7 USE use NN cord-014901-d9szap94 207 8 Table Table NNP cord-014901-d9szap94 207 9 1 1 CD cord-014901-d9szap94 207 10 presents present VBZ cord-014901-d9szap94 207 11 examples example NNS cord-014901-d9szap94 207 12 of of IN cord-014901-d9szap94 207 13 using use VBG cord-014901-d9szap94 207 14 various various JJ cord-014901-d9szap94 207 15 expres expres NNP cord-014901-d9szap94 207 16 sion sion NNP cord-014901-d9szap94 207 17 systems system NNS cord-014901-d9szap94 207 18 for for IN cord-014901-d9szap94 207 19 the the DT cord-014901-d9szap94 207 20 production production NN cord-014901-d9szap94 207 21 of of IN cord-014901-d9szap94 207 22 " " `` cord-014901-d9szap94 207 23 candidate candidate NN cord-014901-d9szap94 207 24 " " '' cord-014901-d9szap94 207 25 plant plant NN cord-014901-d9szap94 207 26 vaccines vaccine NNS cord-014901-d9szap94 207 27 for for IN cord-014901-d9szap94 207 28 veterinary veterinary JJ cord-014901-d9szap94 207 29 use use NN cord-014901-d9szap94 207 30 . . . cord-014901-d9szap94 208 1 Main main JJ cord-014901-d9szap94 208 2 specific specific JJ cord-014901-d9szap94 208 3 immunogenic immunogenic JJ cord-014901-d9szap94 208 4 No no NN cord-014901-d9szap94 208 5 . . NN cord-014901-d9szap94 208 6 1 1 CD cord-014901-d9szap94 208 7 2015 2015 CD cord-014901-d9szap94 208 8 proteins protein NNS cord-014901-d9szap94 208 9 synthesized synthesize VBN cord-014901-d9szap94 208 10 at at IN cord-014901-d9szap94 208 11 respective respective JJ cord-014901-d9szap94 208 12 diseases disease NNS cord-014901-d9szap94 208 13 ( ( -LRB- cord-014901-d9szap94 208 14 structural structural JJ cord-014901-d9szap94 208 15 proteins protein NNS cord-014901-d9szap94 208 16 , , , cord-014901-d9szap94 208 17 hemagglutinins hemagglutinin NNS cord-014901-d9szap94 208 18 , , , cord-014901-d9szap94 208 19 glycoproteins glycoprotein NNS cord-014901-d9szap94 208 20 ) ) -RRB- cord-014901-d9szap94 208 21 are be VBP cord-014901-d9szap94 208 22 usually usually RB cord-014901-d9szap94 208 23 used use VBN cord-014901-d9szap94 208 24 as as IN cord-014901-d9szap94 208 25 antigens antigen NNS cord-014901-d9szap94 208 26 . . . cord-014901-d9szap94 209 1 The the DT cord-014901-d9szap94 209 2 most most RBS cord-014901-d9szap94 209 3 commonly commonly RB cord-014901-d9szap94 209 4 used use VBN cord-014901-d9szap94 209 5 method method NN cord-014901-d9szap94 209 6 of of IN cord-014901-d9szap94 209 7 transgene transgene NN cord-014901-d9szap94 209 8 construct construct NN cord-014901-d9szap94 209 9 delivering deliver VBG cord-014901-d9szap94 209 10 into into IN cord-014901-d9szap94 209 11 plant plant NN cord-014901-d9szap94 209 12 cells cell NNS cord-014901-d9szap94 209 13 is be VBZ cord-014901-d9szap94 209 14 still still RB cord-014901-d9szap94 209 15 the the DT cord-014901-d9szap94 209 16 agrobacterial agrobacterial JJ cord-014901-d9szap94 209 17 transformation transformation NN cord-014901-d9szap94 209 18 . . . cord-014901-d9szap94 210 1 In in IN cord-014901-d9szap94 210 2 some some DT cord-014901-d9szap94 210 3 cases case NNS cord-014901-d9szap94 210 4 , , , cord-014901-d9szap94 210 5 the the DT cord-014901-d9szap94 210 6 level level NN cord-014901-d9szap94 210 7 of of IN cord-014901-d9szap94 210 8 target target NN cord-014901-d9szap94 210 9 protein protein NN cord-014901-d9szap94 210 10 expression expression NN cord-014901-d9szap94 210 11 was be VBD cord-014901-d9szap94 210 12 rather rather RB cord-014901-d9szap94 210 13 high high JJ cord-014901-d9szap94 210 14 [ [ -LRB- cord-014901-d9szap94 210 15 55 55 CD cord-014901-d9szap94 210 16 ] ] -RRB- cord-014901-d9szap94 210 17 [ [ -LRB- cord-014901-d9szap94 210 18 56 56 CD cord-014901-d9szap94 210 19 ] ] -RRB- cord-014901-d9szap94 210 20 [ [ -LRB- cord-014901-d9szap94 210 21 57 57 CD cord-014901-d9szap94 210 22 ] ] -RRB- cord-014901-d9szap94 210 23 [ [ -LRB- cord-014901-d9szap94 210 24 58 58 CD cord-014901-d9szap94 210 25 ] ] -RRB- cord-014901-d9szap94 210 26 , , . cord-014901-d9szap94 211 1 espe espe NNP cord-014901-d9szap94 211 2 cially cially RB cord-014901-d9szap94 211 3 in in IN cord-014901-d9szap94 211 4 the the DT cord-014901-d9szap94 211 5 systems system NNS cord-014901-d9szap94 211 6 of of IN cord-014901-d9szap94 211 7 transient transient JJ cord-014901-d9szap94 211 8 expression expression NN cord-014901-d9szap94 211 9 [ [ -LRB- cord-014901-d9szap94 211 10 59 59 CD cord-014901-d9szap94 211 11 ] ] -RRB- cord-014901-d9szap94 212 1 [ [ -LRB- cord-014901-d9szap94 212 2 60 60 CD cord-014901-d9szap94 212 3 ] ] -RRB- cord-014901-d9szap94 212 4 [ [ -LRB- cord-014901-d9szap94 212 5 61 61 CD cord-014901-d9szap94 212 6 ] ] -RRB- cord-014901-d9szap94 212 7 , , , cord-014901-d9szap94 212 8 and and CC cord-014901-d9szap94 212 9 suitable suitable JJ cord-014901-d9szap94 212 10 for for IN cord-014901-d9szap94 212 11 product product NN cord-014901-d9szap94 212 12 commercialization commercialization NN cord-014901-d9szap94 212 13 . . . cord-014901-d9szap94 213 1 In in IN cord-014901-d9szap94 213 2 all all DT cord-014901-d9szap94 213 3 experiments experiment NNS cord-014901-d9szap94 213 4 using use VBG cord-014901-d9szap94 213 5 the the DT cord-014901-d9szap94 213 6 " " `` cord-014901-d9szap94 213 7 candidate candidate NN cord-014901-d9szap94 213 8 " " '' cord-014901-d9szap94 213 9 plant plant NN cord-014901-d9szap94 213 10 vac vac NNP cord-014901-d9szap94 213 11 cines cines NNP cord-014901-d9szap94 213 12 , , , cord-014901-d9szap94 213 13 the the DT cord-014901-d9szap94 213 14 formation formation NN cord-014901-d9szap94 213 15 of of IN cord-014901-d9szap94 213 16 a a DT cord-014901-d9szap94 213 17 specific specific JJ cord-014901-d9szap94 213 18 immune immune JJ cord-014901-d9szap94 213 19 response response NN cord-014901-d9szap94 213 20 was be VBD cord-014901-d9szap94 213 21 demonstrated demonstrate VBN cord-014901-d9szap94 213 22 in in IN cord-014901-d9szap94 213 23 vivo vivo NN cord-014901-d9szap94 213 24 , , , cord-014901-d9szap94 213 25 and and CC cord-014901-d9szap94 213 26 in in IN cord-014901-d9szap94 213 27 most most JJS cord-014901-d9szap94 213 28 experiments experiment NNS cord-014901-d9szap94 213 29 immunogenic immunogenic JJ cord-014901-d9szap94 213 30 proteins protein NNS cord-014901-d9szap94 213 31 were be VBD cord-014901-d9szap94 213 32 delivered deliver VBN cord-014901-d9szap94 213 33 to to IN cord-014901-d9szap94 213 34 animals animal NNS cord-014901-d9szap94 213 35 just just RB cord-014901-d9szap94 213 36 orally orally RB cord-014901-d9szap94 213 37 . . . cord-014901-d9szap94 213 38 " " '' cord-014901-d9szap94 214 1 Candidate candidate NN cord-014901-d9szap94 214 2 " " `` cord-014901-d9szap94 214 3 plant plant NN cord-014901-d9szap94 214 4 vaccines vaccine NNS cord-014901-d9szap94 214 5 were be VBD cord-014901-d9szap94 214 6 usually usually RB cord-014901-d9szap94 214 7 tested test VBN cord-014901-d9szap94 214 8 on on IN cord-014901-d9szap94 214 9 mice mouse NNS cord-014901-d9szap94 214 10 , , , cord-014901-d9szap94 214 11 but but CC cord-014901-d9szap94 214 12 in in IN cord-014901-d9szap94 214 13 approximately approximately RB cord-014901-d9szap94 214 14 a a DT cord-014901-d9szap94 214 15 quarter quarter NN cord-014901-d9szap94 214 16 works work VBZ cord-014901-d9szap94 214 17 the the DT cord-014901-d9szap94 214 18 ani ani NNP cord-014901-d9szap94 214 19 mals mal NNS cord-014901-d9szap94 214 20 subjected subject VBN cord-014901-d9szap94 214 21 to to IN cord-014901-d9szap94 214 22 the the DT cord-014901-d9szap94 214 23 disease disease NN cord-014901-d9szap94 214 24 were be VBD cord-014901-d9szap94 214 25 tested test VBN cord-014901-d9szap94 214 26 . . . cord-014901-d9szap94 215 1 Protein Protein NNP cord-014901-d9szap94 215 2 S S NNP cord-014901-d9szap94 215 3 of of IN cord-014901-d9szap94 215 4 the the DT cord-014901-d9szap94 215 5 transmissible transmissible JJ cord-014901-d9szap94 215 6 swine swine NN cord-014901-d9szap94 215 7 gastroenteritis gastroenteritis NN cord-014901-d9szap94 215 8 virus virus NN cord-014901-d9szap94 215 9 synthe synthe NNP cord-014901-d9szap94 215 10 sized sized JJ cord-014901-d9szap94 215 11 in in IN cord-014901-d9szap94 215 12 the the DT cord-014901-d9szap94 215 13 cells cell NNS cord-014901-d9szap94 215 14 of of IN cord-014901-d9szap94 215 15 transgenic transgenic JJ cord-014901-d9szap94 215 16 maize maize NN cord-014901-d9szap94 215 17 [ [ -LRB- cord-014901-d9szap94 215 18 55 55 CD cord-014901-d9szap94 215 19 , , , cord-014901-d9szap94 215 20 62 62 CD cord-014901-d9szap94 215 21 , , , cord-014901-d9szap94 215 22 63 63 CD cord-014901-d9szap94 215 23 ] ] -RRB- cord-014901-d9szap94 215 24 or or CC cord-014901-d9szap94 215 25 tobacco tobacco VB cord-014901-d9szap94 215 26 [ [ -LRB- cord-014901-d9szap94 215 27 64 64 CD cord-014901-d9szap94 215 28 ] ] -RRB- cord-014901-d9szap94 215 29 and and CC cord-014901-d9szap94 215 30 delivered deliver VBD cord-014901-d9szap94 215 31 into into IN cord-014901-d9szap94 215 32 the the DT cord-014901-d9szap94 215 33 body body NN cord-014901-d9szap94 215 34 of of IN cord-014901-d9szap94 215 35 pigs pig NNS cord-014901-d9szap94 215 36 as as IN cord-014901-d9szap94 215 37 a a DT cord-014901-d9szap94 215 38 food food NN cord-014901-d9szap94 215 39 supplement supplement NN cord-014901-d9szap94 215 40 , , , cord-014901-d9szap94 215 41 provided provide VBN cord-014901-d9szap94 215 42 for for IN cord-014901-d9szap94 215 43 100 100 CD cord-014901-d9szap94 215 44 % % NN cord-014901-d9szap94 215 45 survival survival NN cord-014901-d9szap94 215 46 of of IN cord-014901-d9szap94 215 47 ani ani NNP cord-014901-d9szap94 215 48 mals mal NNS cord-014901-d9szap94 215 49 after after IN cord-014901-d9szap94 215 50 infection infection NN cord-014901-d9szap94 215 51 [ [ -LRB- cord-014901-d9szap94 215 52 62 62 CD cord-014901-d9szap94 215 53 ] ] -RRB- cord-014901-d9szap94 215 54 . . . cord-014901-d9szap94 216 1 Rabbit rabbit NN cord-014901-d9szap94 216 2 protein protein NN cord-014901-d9szap94 216 3 VP60 VP60 NNP cord-014901-d9szap94 216 4 virus virus NN cord-014901-d9szap94 216 5 synthesized synthesize VBN cord-014901-d9szap94 216 6 in in IN cord-014901-d9szap94 216 7 potato potato NN cord-014901-d9szap94 216 8 [ [ -LRB- cord-014901-d9szap94 216 9 65 65 CD cord-014901-d9szap94 216 10 ] ] -RRB- cord-014901-d9szap94 216 11 and and CC cord-014901-d9szap94 216 12 other other JJ cord-014901-d9szap94 216 13 plants plant NNS cord-014901-d9szap94 216 14 ( ( -LRB- cord-014901-d9szap94 216 15 tobacco tobacco NN cord-014901-d9szap94 216 16 , , , cord-014901-d9szap94 216 17 pea pea NN cord-014901-d9szap94 216 18 , , , cord-014901-d9szap94 216 19 rape rape NN cord-014901-d9szap94 216 20 ) ) -RRB- cord-014901-d9szap94 216 21 [ [ -LRB- cord-014901-d9szap94 216 22 66 66 CD cord-014901-d9szap94 216 23 ] ] -RRB- cord-014901-d9szap94 216 24 and and CC cord-014901-d9szap94 216 25 delivered deliver VBD cord-014901-d9szap94 216 26 orally orally RB cord-014901-d9szap94 216 27 enhanced enhance VBN cord-014901-d9szap94 216 28 protective protective JJ cord-014901-d9szap94 216 29 immunity immunity NN cord-014901-d9szap94 216 30 : : : cord-014901-d9szap94 216 31 after after IN cord-014901-d9szap94 216 32 infecting infect VBG cord-014901-d9szap94 216 33 rabbits rabbit NNS cord-014901-d9szap94 216 34 with with IN cord-014901-d9szap94 216 35 this this DT cord-014901-d9szap94 216 36 virus virus NN cord-014901-d9szap94 216 37 all all DT cord-014901-d9szap94 216 38 ani ani NNP cord-014901-d9szap94 216 39 mals mal NNS cord-014901-d9szap94 216 40 survived survive VBD cord-014901-d9szap94 216 41 [ [ -LRB- cord-014901-d9szap94 216 42 66 66 CD cord-014901-d9szap94 216 43 ] ] -RRB- cord-014901-d9szap94 216 44 . . . cord-014901-d9szap94 217 1 The the DT cord-014901-d9szap94 217 2 effect effect NN cord-014901-d9szap94 217 3 of of IN cord-014901-d9szap94 217 4 plant plant NN cord-014901-d9szap94 217 5 recombinant recombinant JJ cord-014901-d9szap94 217 6 antigen antigen NN cord-014901-d9szap94 217 7 was be VBD cord-014901-d9szap94 217 8 comparable comparable JJ cord-014901-d9szap94 217 9 with with IN cord-014901-d9szap94 217 10 commercially commercially RB cord-014901-d9szap94 217 11 used use VBN cord-014901-d9szap94 217 12 vac vac NN cord-014901-d9szap94 217 13 cines cines NNP cord-014901-d9szap94 217 14 . . . cord-014901-d9szap94 218 1 The the DT cord-014901-d9szap94 218 2 use use NN cord-014901-d9szap94 218 3 of of IN cord-014901-d9szap94 218 4 plant plant NN cord-014901-d9szap94 218 5 vaccines vaccine NNS cord-014901-d9szap94 218 6 for for IN cord-014901-d9szap94 218 7 the the DT cord-014901-d9szap94 218 8 vaccination vaccination NN cord-014901-d9szap94 218 9 of of IN cord-014901-d9szap94 218 10 wild wild JJ cord-014901-d9szap94 218 11 animals animal NNS cord-014901-d9szap94 218 12 using use VBG cord-014901-d9szap94 218 13 edible edible JJ cord-014901-d9szap94 218 14 baits bait NNS cord-014901-d9szap94 218 15 ( ( -LRB- cord-014901-d9szap94 218 16 e.g. e.g. RB cord-014901-d9szap94 218 17 , , , cord-014901-d9szap94 218 18 vaccine vaccine NN cord-014901-d9szap94 218 19 against against IN cord-014901-d9szap94 218 20 rabies rabies NN cord-014901-d9szap94 218 21 ) ) -RRB- cord-014901-d9szap94 218 22 will will MD cord-014901-d9szap94 218 23 lead lead VB cord-014901-d9szap94 218 24 to to IN cord-014901-d9szap94 218 25 an an DT cord-014901-d9szap94 218 26 increase increase NN cord-014901-d9szap94 218 27 in in IN cord-014901-d9szap94 218 28 the the DT cord-014901-d9szap94 218 29 proportion proportion NN cord-014901-d9szap94 218 30 of of IN cord-014901-d9szap94 218 31 wild wild JJ cord-014901-d9szap94 218 32 animal animal NN cord-014901-d9szap94 218 33 populations population NNS cord-014901-d9szap94 218 34 having have VBG cord-014901-d9szap94 218 35 immunity immunity NN cord-014901-d9szap94 218 36 to to IN cord-014901-d9szap94 218 37 the the DT cord-014901-d9szap94 218 38 rabies rabies NN cord-014901-d9szap94 218 39 virus virus NN cord-014901-d9szap94 218 40 . . . cord-014901-d9szap94 219 1 A a DT cord-014901-d9szap94 219 2 potential potential JJ cord-014901-d9szap94 219 3 possibility possibility NN cord-014901-d9szap94 219 4 to to TO cord-014901-d9szap94 219 5 reduce reduce VB cord-014901-d9szap94 219 6 the the DT cord-014901-d9szap94 219 7 cost cost NN cord-014901-d9szap94 219 8 of of IN cord-014901-d9szap94 219 9 produc produc NN cord-014901-d9szap94 219 10 tion tion NN cord-014901-d9szap94 219 11 of of IN cord-014901-d9szap94 219 12 biopharmaceutics biopharmaceutics NNP cord-014901-d9szap94 219 13 using use VBG cord-014901-d9szap94 219 14 genetically genetically RB cord-014901-d9szap94 219 15 modified modify VBN cord-014901-d9szap94 219 16 plants plant NNS cord-014901-d9szap94 219 17 served serve VBD cord-014901-d9szap94 219 18 at at IN cord-014901-d9szap94 219 19 the the DT cord-014901-d9szap94 219 20 end end NN cord-014901-d9szap94 219 21 of of IN cord-014901-d9szap94 219 22 the the DT cord-014901-d9szap94 219 23 XX XX NNP cord-014901-d9szap94 219 24 century century NN cord-014901-d9szap94 219 25 as as IN cord-014901-d9szap94 219 26 an an DT cord-014901-d9szap94 219 27 impe impe NNP cord-014901-d9szap94 219 28 tus tus XX cord-014901-d9szap94 219 29 for for IN cord-014901-d9szap94 219 30 more more JJR cord-014901-d9szap94 219 31 than than IN cord-014901-d9szap94 219 32 twenty twenty CD cord-014901-d9szap94 219 33 biotechnological biotechnological JJ cord-014901-d9szap94 219 34 companies company NNS cord-014901-d9szap94 219 35 to to TO cord-014901-d9szap94 219 36 initiate initiate VB cord-014901-d9szap94 219 37 commercial commercial JJ cord-014901-d9szap94 219 38 programs program NNS cord-014901-d9szap94 219 39 . . . cord-014901-d9szap94 220 1 As as IN cord-014901-d9szap94 220 2 seen see VBN cord-014901-d9szap94 220 3 from from IN cord-014901-d9szap94 220 4 the the DT cord-014901-d9szap94 220 5 Table Table NNP cord-014901-d9szap94 220 6 1 1 CD cord-014901-d9szap94 220 7 , , , cord-014901-d9szap94 220 8 many many JJ cord-014901-d9szap94 220 9 biological biological JJ cord-014901-d9szap94 220 10 products product NNS cord-014901-d9szap94 220 11 of of IN cord-014901-d9szap94 220 12 plant plant NN cord-014901-d9szap94 220 13 origin origin NN cord-014901-d9szap94 220 14 are be VBP cord-014901-d9szap94 220 15 developed develop VBN cord-014901-d9szap94 220 16 , , , cord-014901-d9szap94 220 17 expressed express VBN cord-014901-d9szap94 220 18 in in IN cord-014901-d9szap94 220 19 different different JJ cord-014901-d9szap94 220 20 types type NNS cord-014901-d9szap94 220 21 of of IN cord-014901-d9szap94 220 22 plants plant NNS cord-014901-d9szap94 220 23 and and CC cord-014901-d9szap94 220 24 plant plant NN cord-014901-d9szap94 220 25 cell cell NN cord-014901-d9szap94 220 26 cultures culture NNS cord-014901-d9szap94 220 27 . . . cord-014901-d9szap94 221 1 For for IN cord-014901-d9szap94 221 2 a a DT cord-014901-d9szap94 221 3 variety variety NN cord-014901-d9szap94 221 4 of of IN cord-014901-d9szap94 221 5 reasons reason NNS cord-014901-d9szap94 221 6 , , , cord-014901-d9szap94 221 7 including include VBG cord-014901-d9szap94 221 8 the the DT cord-014901-d9szap94 221 9 still still RB cord-014901-d9szap94 221 10 skeptical skeptical JJ cord-014901-d9szap94 221 11 attitude attitude NN cord-014901-d9szap94 221 12 of of IN cord-014901-d9szap94 221 13 the the DT cord-014901-d9szap94 221 14 human human JJ cord-014901-d9szap94 221 15 community community NN cord-014901-d9szap94 221 16 to to IN cord-014901-d9szap94 221 17 the the DT cord-014901-d9szap94 221 18 biosafety biosafety NN cord-014901-d9szap94 221 19 of of IN cord-014901-d9szap94 221 20 genetically genetically RB cord-014901-d9szap94 221 21 modified modify VBN cord-014901-d9szap94 221 22 plants plant NNS cord-014901-d9szap94 221 23 , , , cord-014901-d9szap94 221 24 many many JJ cord-014901-d9szap94 221 25 of of IN cord-014901-d9szap94 221 26 these these DT cord-014901-d9szap94 221 27 works work NNS cord-014901-d9szap94 221 28 remained remain VBD cord-014901-d9szap94 221 29 in in IN cord-014901-d9szap94 221 30 the the DT cord-014901-d9szap94 221 31 framework framework NN cord-014901-d9szap94 221 32 of of IN cord-014901-d9szap94 221 33 laboratory laboratory NN cord-014901-d9szap94 221 34 tests test NNS cord-014901-d9szap94 221 35 . . . cord-014901-d9szap94 222 1 At at IN cord-014901-d9szap94 222 2 the the DT cord-014901-d9szap94 222 3 moment moment NN cord-014901-d9szap94 222 4 three three CD cord-014901-d9szap94 222 5 companies company NNS cord-014901-d9szap94 222 6 function function VBP cord-014901-d9szap94 222 7 on on IN cord-014901-d9szap94 222 8 the the DT cord-014901-d9szap94 222 9 biotechnology biotechnology NN cord-014901-d9szap94 222 10 market market NN cord-014901-d9szap94 222 11 of of IN cord-014901-d9szap94 222 12 veterinary veterinary JJ cord-014901-d9szap94 222 13 preparations preparation NNS cord-014901-d9szap94 222 14 , , , cord-014901-d9szap94 222 15 two two CD cord-014901-d9szap94 222 16 from from IN cord-014901-d9szap94 222 17 the the DT cord-014901-d9szap94 222 18 United United NNP cord-014901-d9szap94 222 19 States States NNP cord-014901-d9szap94 222 20 and and CC cord-014901-d9szap94 222 21 one one CD cord-014901-d9szap94 222 22 from from IN cord-014901-d9szap94 222 23 Canada Canada NNP cord-014901-d9szap94 222 24 ( ( -LRB- cord-014901-d9szap94 222 25 Table Table NNP cord-014901-d9szap94 222 26 2 2 CD cord-014901-d9szap94 222 27 ) ) -RRB- cord-014901-d9szap94 222 28 . . . cord-014901-d9szap94 223 1 In in IN cord-014901-d9szap94 223 2 the the DT cord-014901-d9szap94 223 3 United United NNP cord-014901-d9szap94 223 4 States States NNP cord-014901-d9szap94 223 5 the the DT cord-014901-d9szap94 223 6 Dow Dow NNP cord-014901-d9szap94 223 7 Agro Agro NNP cord-014901-d9szap94 223 8 Sciences Sciences NNPS cord-014901-d9szap94 223 9 company company NN cord-014901-d9szap94 223 10 presented present VBD cord-014901-d9szap94 223 11 a a DT cord-014901-d9szap94 223 12 recombinant recombinant JJ cord-014901-d9szap94 223 13 plant plant NN cord-014901-d9szap94 223 14 viral viral JJ cord-014901-d9szap94 223 15 HN HN NNP cord-014901-d9szap94 223 16 protein protein NN cord-014901-d9szap94 223 17 of of IN cord-014901-d9szap94 223 18 the the DT cord-014901-d9szap94 223 19 Newcastle Newcastle NNP cord-014901-d9szap94 223 20 disease disease NN cord-014901-d9szap94 223 21 virus virus NN cord-014901-d9szap94 223 22 ( ( -LRB- cord-014901-d9szap94 223 23 approved approve VBN cord-014901-d9szap94 223 24 by by IN cord-014901-d9szap94 223 25 USDA USDA NNP cord-014901-d9szap94 223 26 ) ) -RRB- cord-014901-d9szap94 223 27 and and CC cord-014901-d9szap94 223 28 a a DT cord-014901-d9szap94 223 29 mixture mixture NN cord-014901-d9szap94 223 30 of of IN cord-014901-d9szap94 223 31 antiviral antiviral JJ cord-014901-d9szap94 223 32 vaccines vaccine NNS cord-014901-d9szap94 223 33 at at IN cord-014901-d9szap94 223 34 the the DT cord-014901-d9szap94 223 35 first first JJ cord-014901-d9szap94 223 36 stage stage NN cord-014901-d9szap94 223 37 of of IN cord-014901-d9szap94 223 38 clinical clinical JJ cord-014901-d9szap94 223 39 trials trial NNS cord-014901-d9szap94 223 40 . . . cord-014901-d9szap94 224 1 The the DT cord-014901-d9szap94 224 2 second second JJ cord-014901-d9szap94 224 3 American american JJ cord-014901-d9szap94 224 4 company company NN cord-014901-d9szap94 224 5 at at IN cord-014901-d9szap94 224 6 Thomas Thomas NNP cord-014901-d9szap94 224 7 Jef Jef NNP cord-014901-d9szap94 224 8 ferson ferson NNP cord-014901-d9szap94 224 9 University University NNP cord-014901-d9szap94 224 10 has have VBZ cord-014901-d9szap94 224 11 developed develop VBN cord-014901-d9szap94 224 12 a a DT cord-014901-d9szap94 224 13 plant plant NN cord-014901-d9szap94 224 14 anti anti AFX cord-014901-d9szap94 224 15 rabies rabies NN cord-014901-d9szap94 224 16 vaccine vaccine NN cord-014901-d9szap94 224 17 ( ( -LRB- cord-014901-d9szap94 224 18 completion completion NN cord-014901-d9szap94 224 19 of of IN cord-014901-d9szap94 224 20 phase phase NN cord-014901-d9szap94 224 21 1 1 CD cord-014901-d9szap94 224 22 ) ) -RRB- cord-014901-d9szap94 224 23 . . . cord-014901-d9szap94 225 1 The the DT cord-014901-d9szap94 225 2 Canadian Canadian NNP cord-014901-d9szap94 225 3 Guardian Guardian NNP cord-014901-d9szap94 225 4 Biosciences Biosciences NNP cord-014901-d9szap94 225 5 company company NN cord-014901-d9szap94 225 6 presented present VBD cord-014901-d9szap94 225 7 plant plant NN cord-014901-d9szap94 225 8 vac vac NNP cord-014901-d9szap94 225 9 cine cine NNP cord-014901-d9szap94 225 10 against against IN cord-014901-d9szap94 225 11 chicken chicken NNP cord-014901-d9szap94 225 12 coccidiosis coccidiosis NNP cord-014901-d9szap94 225 13 at at IN cord-014901-d9szap94 225 14 the the DT cord-014901-d9szap94 225 15 second second JJ cord-014901-d9szap94 225 16 phase phase NN cord-014901-d9szap94 225 17 of of IN cord-014901-d9szap94 225 18 clinical clinical JJ cord-014901-d9szap94 225 19 trials trial NNS cord-014901-d9szap94 225 20 . . . cord-014901-d9szap94 226 1 Production production NN cord-014901-d9szap94 226 2 and and CC cord-014901-d9szap94 226 3 wide wide JJ cord-014901-d9szap94 226 4 distribution distribution NN cord-014901-d9szap94 226 5 of of IN cord-014901-d9szap94 226 6 biopharmaceu biopharmaceu NNP cord-014901-d9szap94 226 7 ticals ticals NNP cord-014901-d9szap94 226 8 is be VBZ cord-014901-d9szap94 226 9 hampered hamper VBN cord-014901-d9szap94 226 10 by by IN cord-014901-d9szap94 226 11 a a DT cord-014901-d9szap94 226 12 number number NN cord-014901-d9szap94 226 13 of of IN cord-014901-d9szap94 226 14 circumstances circumstance NNS cord-014901-d9szap94 226 15 . . . cord-014901-d9szap94 227 1 The the DT cord-014901-d9szap94 227 2 first first JJ cord-014901-d9szap94 227 3 of of IN cord-014901-d9szap94 227 4 them -PRON- PRP cord-014901-d9szap94 227 5 is be VBZ cord-014901-d9szap94 227 6 related relate VBN cord-014901-d9szap94 227 7 to to IN cord-014901-d9szap94 227 8 the the DT cord-014901-d9szap94 227 9 problem problem NN cord-014901-d9szap94 227 10 of of IN cord-014901-d9szap94 227 11 biosafetythe biosafetythe NNP cord-014901-d9szap94 227 12 cultivation cultivation NN cord-014901-d9szap94 227 13 of of IN cord-014901-d9szap94 227 14 genetically genetically RB cord-014901-d9szap94 227 15 modified modify VBN cord-014901-d9szap94 227 16 plants plant NNS cord-014901-d9szap94 227 17 in in IN cord-014901-d9szap94 227 18 the the DT cord-014901-d9szap94 227 19 field field NN cord-014901-d9szap94 227 20 can can MD cord-014901-d9szap94 227 21 lead lead VB cord-014901-d9szap94 227 22 to to IN cord-014901-d9szap94 227 23 the the DT cord-014901-d9szap94 227 24 accidental accidental JJ cord-014901-d9szap94 227 25 introduction introduction NN cord-014901-d9szap94 227 26 of of IN cord-014901-d9szap94 227 27 foreign foreign JJ cord-014901-d9szap94 227 28 genes gene NNS cord-014901-d9szap94 227 29 into into IN cord-014901-d9szap94 227 30 crops crop NNS cord-014901-d9szap94 227 31 grown grow VBN cord-014901-d9szap94 227 32 for for IN cord-014901-d9szap94 227 33 human human JJ cord-014901-d9szap94 227 34 consumption consumption NN cord-014901-d9szap94 227 35 . . . cord-014901-d9szap94 228 1 Therefore therefore RB cord-014901-d9szap94 228 2 , , , cord-014901-d9szap94 228 3 most most JJS cord-014901-d9szap94 228 4 companies company NNS cord-014901-d9szap94 228 5 producing produce VBG cord-014901-d9szap94 228 6 biopharma biopharma NNP cord-014901-d9szap94 228 7 ceutics ceutics NNP cord-014901-d9szap94 228 8 focused focus VBN cord-014901-d9szap94 228 9 on on IN cord-014901-d9szap94 228 10 plant plant NN cord-014901-d9szap94 228 11 species specie NNS cord-014901-d9szap94 228 12 , , , cord-014901-d9szap94 228 13 which which WDT cord-014901-d9szap94 228 14 are be VBP cord-014901-d9szap94 228 15 absent absent JJ cord-014901-d9szap94 228 16 from from IN cord-014901-d9szap94 228 17 the the DT cord-014901-d9szap94 228 18 food food NN cord-014901-d9szap94 228 19 chain chain NN cord-014901-d9szap94 228 20 of of IN cord-014901-d9szap94 228 21 humans human NNS cord-014901-d9szap94 228 22 and and CC cord-014901-d9szap94 228 23 animals animal NNS cord-014901-d9szap94 228 24 and and CC cord-014901-d9szap94 228 25 also also RB cord-014901-d9szap94 228 26 on on IN cord-014901-d9szap94 228 27 growing grow VBG cord-014901-d9szap94 228 28 of of IN cord-014901-d9szap94 228 29 genetically genetically RB cord-014901-d9szap94 228 30 modified modify VBN cord-014901-d9szap94 228 31 plants plant NNS cord-014901-d9szap94 228 32 preventing prevent VBG cord-014901-d9szap94 228 33 their -PRON- PRP$ cord-014901-d9szap94 228 34 cross cross NN cord-014901-d9szap94 228 35 pollination pollination NN cord-014901-d9szap94 228 36 with with IN cord-014901-d9szap94 228 37 other other JJ cord-014901-d9szap94 228 38 crops crop NNS cord-014901-d9szap94 228 39 . . . cord-014901-d9szap94 229 1 The the DT cord-014901-d9szap94 229 2 second second JJ cord-014901-d9szap94 229 3 difficulty difficulty NN cord-014901-d9szap94 229 4 is be VBZ cord-014901-d9szap94 229 5 related relate VBN cord-014901-d9szap94 229 6 to to IN cord-014901-d9szap94 229 7 the the DT cord-014901-d9szap94 229 8 necessity necessity NN cord-014901-d9szap94 229 9 of of IN cord-014901-d9szap94 229 10 plant plant NN cord-014901-d9szap94 229 11 material material NN cord-014901-d9szap94 229 12 treatment treatment NN cord-014901-d9szap94 229 13 for for IN cord-014901-d9szap94 229 14 the the DT cord-014901-d9szap94 229 15 removal removal NN cord-014901-d9szap94 229 16 of of IN cord-014901-d9szap94 229 17 various various JJ cord-014901-d9szap94 229 18 undesired undesired JJ cord-014901-d9szap94 229 19 com com NN cord-014901-d9szap94 229 20 pounds pound NNS cord-014901-d9szap94 229 21 , , , cord-014901-d9szap94 229 22 such such JJ cord-014901-d9szap94 229 23 as as IN cord-014901-d9szap94 229 24 lignin lignin NN cord-014901-d9szap94 229 25 , , , cord-014901-d9szap94 229 26 proteases protease NNS cord-014901-d9szap94 229 27 , , , cord-014901-d9szap94 229 28 phenolic phenolic JJ cord-014901-d9szap94 229 29 com com NN cord-014901-d9szap94 229 30 pounds pound NNS cord-014901-d9szap94 229 31 , , , cord-014901-d9szap94 229 32 and and CC cord-014901-d9szap94 229 33 pigments pigment NNS cord-014901-d9szap94 229 34 , , , cord-014901-d9szap94 229 35 especially especially RB cord-014901-d9szap94 229 36 in in IN cord-014901-d9szap94 229 37 the the DT cord-014901-d9szap94 229 38 case case NN cord-014901-d9szap94 229 39 of of IN cord-014901-d9szap94 229 40 plant plant NN cord-014901-d9szap94 229 41 species specie NNS cord-014901-d9szap94 229 42 , , , cord-014901-d9szap94 229 43 which which WDT cord-014901-d9szap94 229 44 are be VBP cord-014901-d9szap94 229 45 not not RB cord-014901-d9szap94 229 46 consumed consume VBN cord-014901-d9szap94 229 47 . . . cord-014901-d9szap94 230 1 All all PDT cord-014901-d9szap94 230 2 these these DT cord-014901-d9szap94 230 3 facts fact NNS cord-014901-d9szap94 230 4 result result VBP cord-014901-d9szap94 230 5 in in IN cord-014901-d9szap94 230 6 the the DT cord-014901-d9szap94 230 7 requirement requirement NN cord-014901-d9szap94 230 8 of of IN cord-014901-d9szap94 230 9 additional additional JJ cord-014901-d9szap94 230 10 studies study NNS cord-014901-d9szap94 230 11 . . . cord-014901-d9szap94 231 1 The the DT cord-014901-d9szap94 231 2 third third JJ cord-014901-d9szap94 231 3 cir cir NNP cord-014901-d9szap94 231 4 cumstance cumstance NNP cord-014901-d9szap94 231 5 is be VBZ cord-014901-d9szap94 231 6 due due JJ cord-014901-d9szap94 231 7 to to IN cord-014901-d9szap94 231 8 the the DT cord-014901-d9szap94 231 9 fact fact NN cord-014901-d9szap94 231 10 that that IN cord-014901-d9szap94 231 11 until until IN cord-014901-d9szap94 231 12 now now RB cord-014901-d9szap94 231 13 all all DT cord-014901-d9szap94 231 14 aspects aspect NNS cord-014901-d9szap94 231 15 of of IN cord-014901-d9szap94 231 16 maintaining maintain VBG cord-014901-d9szap94 231 17 and and CC cord-014901-d9szap94 231 18 growing growing NN cord-014901-d9szap94 231 19 of of IN cord-014901-d9szap94 231 20 plants plant NNS cord-014901-d9szap94 231 21 producing produce VBG cord-014901-d9szap94 231 22 biop biop NNP cord-014901-d9szap94 231 23 harmaceuticals harmaceutical NNS cord-014901-d9szap94 231 24 are be VBP cord-014901-d9szap94 231 25 not not RB cord-014901-d9szap94 231 26 settled settle VBN cord-014901-d9szap94 231 27 at at IN cord-014901-d9szap94 231 28 the the DT cord-014901-d9szap94 231 29 legislative legislative JJ cord-014901-d9szap94 231 30 level level NN cord-014901-d9szap94 231 31 . . . cord-014901-d9szap94 232 1 Ambiguity ambiguity NN cord-014901-d9szap94 232 2 and and CC cord-014901-d9szap94 232 3 vagueness vagueness NN cord-014901-d9szap94 232 4 of of IN cord-014901-d9szap94 232 5 the the DT cord-014901-d9szap94 232 6 existing exist VBG cord-014901-d9szap94 232 7 legislation legislation NN cord-014901-d9szap94 232 8 in in IN cord-014901-d9szap94 232 9 this this DT cord-014901-d9szap94 232 10 area area NN cord-014901-d9szap94 232 11 lead lead VB cord-014901-d9szap94 232 12 to to IN cord-014901-d9szap94 232 13 the the DT cord-014901-d9szap94 232 14 fact fact NN cord-014901-d9szap94 232 15 that that IN cord-014901-d9szap94 232 16 large large JJ cord-014901-d9szap94 232 17 biopharmaceutical biopharmaceutical JJ cord-014901-d9szap94 232 18 and and CC cord-014901-d9szap94 232 19 biotechnology biotechnology NN cord-014901-d9szap94 232 20 companies company NNS cord-014901-d9szap94 232 21 do do VBP cord-014901-d9szap94 232 22 not not RB cord-014901-d9szap94 232 23 tend tend VB cord-014901-d9szap94 232 24 to to TO cord-014901-d9szap94 232 25 invest invest VB cord-014901-d9szap94 232 26 in in IN cord-014901-d9szap94 232 27 the the DT cord-014901-d9szap94 232 28 development development NN cord-014901-d9szap94 232 29 of of IN cord-014901-d9szap94 232 30 technological technological JJ cord-014901-d9szap94 232 31 lines line NNS cord-014901-d9szap94 232 32 and and CC cord-014901-d9szap94 232 33 research research NN cord-014901-d9szap94 232 34 programs program NNS cord-014901-d9szap94 232 35 in in IN cord-014901-d9szap94 232 36 this this DT cord-014901-d9szap94 232 37 area area NN cord-014901-d9szap94 232 38 , , , cord-014901-d9szap94 232 39 which which WDT cord-014901-d9szap94 232 40 significantly significantly RB cord-014901-d9szap94 232 41 inhibits inhibit VBZ cord-014901-d9szap94 232 42 the the DT cord-014901-d9szap94 232 43 development development NN cord-014901-d9szap94 232 44 of of IN cord-014901-d9szap94 232 45 the the DT cord-014901-d9szap94 232 46 industry industry NN cord-014901-d9szap94 232 47 . . . cord-014901-d9szap94 233 1 A a DT cord-014901-d9szap94 233 2 significant significant JJ cord-014901-d9szap94 233 3 problem problem NN cord-014901-d9szap94 233 4 for for IN cord-014901-d9szap94 233 5 the the DT cord-014901-d9szap94 233 6 development development NN cord-014901-d9szap94 233 7 of of IN cord-014901-d9szap94 233 8 vac vac NNP cord-014901-d9szap94 233 9 cines cines NNP cord-014901-d9szap94 233 10 for for IN cord-014901-d9szap94 233 11 veterinary veterinary JJ cord-014901-d9szap94 233 12 use use NN cord-014901-d9szap94 233 13 , , , cord-014901-d9szap94 233 14 especially especially RB cord-014901-d9szap94 233 15 those those DT cord-014901-d9szap94 233 16 used use VBN cord-014901-d9szap94 233 17 in in IN cord-014901-d9szap94 233 18 agri agri NN cord-014901-d9szap94 233 19 culture culture NN cord-014901-d9szap94 233 20 , , , cord-014901-d9szap94 233 21 is be VBZ cord-014901-d9szap94 233 22 the the DT cord-014901-d9szap94 233 23 need need NN cord-014901-d9szap94 233 24 to to TO cord-014901-d9szap94 233 25 minimize minimize VB cord-014901-d9szap94 233 26 the the DT cord-014901-d9szap94 233 27 price price NN cord-014901-d9szap94 233 28 of of IN cord-014901-d9szap94 233 29 the the DT cord-014901-d9szap94 233 30 final final JJ cord-014901-d9szap94 233 31 product product NN cord-014901-d9szap94 233 32 . . . cord-014901-d9szap94 234 1 The the DT cord-014901-d9szap94 234 2 vaccine vaccine NN cord-014901-d9szap94 234 3 should should MD cord-014901-d9szap94 234 4 be be VB cord-014901-d9szap94 234 5 inexpensive inexpensive JJ cord-014901-d9szap94 234 6 for for IN cord-014901-d9szap94 234 7 entre entre NNP cord-014901-d9szap94 234 8 preneurs preneurs NNP cord-014901-d9szap94 234 9 engaged engage VBN cord-014901-d9szap94 234 10 in in IN cord-014901-d9szap94 234 11 commercial commercial JJ cord-014901-d9szap94 234 12 animal animal NN cord-014901-d9szap94 234 13 breeding breeding NN cord-014901-d9szap94 234 14 and and CC cord-014901-d9szap94 234 15 fully fully RB cord-014901-d9szap94 234 16 subsidized subsidize VBN cord-014901-d9szap94 234 17 , , , cord-014901-d9szap94 234 18 if if IN cord-014901-d9szap94 234 19 you -PRON- PRP cord-014901-d9szap94 234 20 intend intend VBP cord-014901-d9szap94 234 21 to to TO cord-014901-d9szap94 234 22 use use VB cord-014901-d9szap94 234 23 the the DT cord-014901-d9szap94 234 24 vaccine vaccine NN cord-014901-d9szap94 234 25 for for IN cord-014901-d9szap94 234 26 mass mass NN cord-014901-d9szap94 234 27 immunization immunization NN cord-014901-d9szap94 234 28 and and CC cord-014901-d9szap94 234 29 the the DT cord-014901-d9szap94 234 30 prevention prevention NN cord-014901-d9szap94 234 31 of of IN cord-014901-d9szap94 234 32 the the DT cord-014901-d9szap94 234 33 disease disease NN cord-014901-d9szap94 234 34 spread spread VBD cord-014901-d9szap94 234 35 in in IN cord-014901-d9szap94 234 36 underdeveloped underdeveloped JJ cord-014901-d9szap94 234 37 regions region NNS cord-014901-d9szap94 234 38 . . . cord-014901-d9szap94 235 1 As as IN cord-014901-d9szap94 235 2 a a DT cord-014901-d9szap94 235 3 result result NN cord-014901-d9szap94 235 4 , , , cord-014901-d9szap94 235 5 the the DT cord-014901-d9szap94 235 6 potential potential JJ cord-014901-d9szap94 235 7 income income NN cord-014901-d9szap94 235 8 of of IN cord-014901-d9szap94 235 9 manufacturers manufacturer NNS cord-014901-d9szap94 235 10 of of IN cord-014901-d9szap94 235 11 vaccines vaccine NNS cord-014901-d9szap94 235 12 for for IN cord-014901-d9szap94 235 13 ani ani NNP cord-014901-d9szap94 235 14 mals mals NNPS cord-014901-d9szap94 235 15 is be VBZ cord-014901-d9szap94 235 16 much much RB cord-014901-d9szap94 235 17 less less JJR cord-014901-d9szap94 235 18 than than IN cord-014901-d9szap94 235 19 that that DT cord-014901-d9szap94 235 20 for for IN cord-014901-d9szap94 235 21 vaccines vaccine NNS cord-014901-d9szap94 235 22 intended intend VBN cord-014901-d9szap94 235 23 for for IN cord-014901-d9szap94 235 24 humans human NNS cord-014901-d9szap94 235 25 . . . cord-014901-d9szap94 236 1 For for IN cord-014901-d9szap94 236 2 example example NN cord-014901-d9szap94 236 3 , , , cord-014901-d9szap94 236 4 in in IN cord-014901-d9szap94 236 5 2007 2007 CD cord-014901-d9szap94 236 6 , , , cord-014901-d9szap94 236 7 the the DT cord-014901-d9szap94 236 8 volume volume NN cord-014901-d9szap94 236 9 of of IN cord-014901-d9szap94 236 10 the the DT cord-014901-d9szap94 236 11 mar mar NNP cord-014901-d9szap94 236 12 ket ket NNP cord-014901-d9szap94 236 13 for for IN cord-014901-d9szap94 236 14 the the DT cord-014901-d9szap94 236 15 vaccine vaccine NN cord-014901-d9szap94 236 16 against against IN cord-014901-d9szap94 236 17 human human JJ cord-014901-d9szap94 236 18 papilloma papilloma NN cord-014901-d9szap94 236 19 virus virus NN cord-014901-d9szap94 236 20 was be VBD cord-014901-d9szap94 236 21 estimated estimate VBN cord-014901-d9szap94 236 22 at at IN cord-014901-d9szap94 236 23 more more JJR cord-014901-d9szap94 236 24 than than IN cord-014901-d9szap94 236 25 1 1 CD cord-014901-d9szap94 236 26 billion billion CD cord-014901-d9szap94 236 27 dollars dollar NNS cord-014901-d9szap94 236 28 , , , cord-014901-d9szap94 236 29 but but CC cord-014901-d9szap94 236 30 the the DT cord-014901-d9szap94 236 31 mar mar NNP cord-014901-d9szap94 236 32 ket ket NNP cord-014901-d9szap94 236 33 for for IN cord-014901-d9szap94 236 34 the the DT cord-014901-d9szap94 236 35 most most RBS cord-014901-d9szap94 236 36 popular popular JJ cord-014901-d9szap94 236 37 animal animal NN cord-014901-d9szap94 236 38 vaccines vaccine NNS cord-014901-d9szap94 236 39 ( ( -LRB- cord-014901-d9szap94 236 40 against against IN cord-014901-d9szap94 236 41 foot foot NN cord-014901-d9szap94 236 42 and and CC cord-014901-d9szap94 236 43 mouth mouth NN cord-014901-d9szap94 236 44 disease disease NN cord-014901-d9szap94 236 45 of of IN cord-014901-d9szap94 236 46 cattle cattle NNS cord-014901-d9szap94 236 47 and and CC cord-014901-d9szap94 236 48 against against IN cord-014901-d9szap94 236 49 Mycoplasma Mycoplasma NNP cord-014901-d9szap94 236 50 hyopneumoniae hyopneumoniae NN cord-014901-d9szap94 236 51 in in IN cord-014901-d9szap94 236 52 pigs pig NNS cord-014901-d9szap94 236 53 ) ) -RRB- cord-014901-d9szap94 236 54 together together RB cord-014901-d9szap94 236 55 amounted amount VBD cord-014901-d9szap94 236 56 to to IN cord-014901-d9szap94 236 57 only only RB cord-014901-d9szap94 236 58 10 10 CD cord-014901-d9szap94 236 59 - - SYM cord-014901-d9szap94 236 60 20 20 CD cord-014901-d9szap94 236 61 % % NN cord-014901-d9szap94 236 62 of of IN cord-014901-d9szap94 236 63 this this DT cord-014901-d9szap94 236 64 amount amount NN cord-014901-d9szap94 236 65 [ [ -LRB- cord-014901-d9szap94 236 66 3 3 CD cord-014901-d9szap94 236 67 ] ] -RRB- cord-014901-d9szap94 236 68 . . . cord-014901-d9szap94 237 1 Animal animal NN cord-014901-d9szap94 237 2 vaccines vaccine NNS cord-014901-d9szap94 237 3 are be VBP cord-014901-d9szap94 237 4 cheaper cheap JJR cord-014901-d9szap94 237 5 and and CC cord-014901-d9szap94 237 6 the the DT cord-014901-d9szap94 237 7 volume volume NN cord-014901-d9szap94 237 8 of of IN cord-014901-d9szap94 237 9 market market NN cord-014901-d9szap94 237 10 for for IN cord-014901-d9szap94 237 11 them -PRON- PRP cord-014901-d9szap94 237 12 is be VBZ cord-014901-d9szap94 237 13 less less JJR cord-014901-d9szap94 237 14 ; ; : cord-014901-d9szap94 237 15 therefore therefore RB cord-014901-d9szap94 237 16 , , , cord-014901-d9szap94 237 17 the the DT cord-014901-d9szap94 237 18 investments investment NNS cord-014901-d9szap94 237 19 in in IN cord-014901-d9szap94 237 20 their -PRON- PRP$ cord-014901-d9szap94 237 21 development development NN cord-014901-d9szap94 237 22 are be VBP cord-014901-d9szap94 237 23 substantially substantially RB cord-014901-d9szap94 237 24 less less JJR cord-014901-d9szap94 237 25 as as IN cord-014901-d9szap94 237 26 compared compare VBN cord-014901-d9szap94 237 27 with with IN cord-014901-d9szap94 237 28 investments investment NNS cord-014901-d9szap94 237 29 in in IN cord-014901-d9szap94 237 30 the the DT cord-014901-d9szap94 237 31 production production NN cord-014901-d9szap94 237 32 of of IN cord-014901-d9szap94 237 33 vaccines vaccine NNS cord-014901-d9szap94 237 34 for for IN cord-014901-d9szap94 237 35 humans human NNS cord-014901-d9szap94 237 36 , , , cord-014901-d9szap94 237 37 whereas whereas IN cord-014901-d9szap94 237 38 the the DT cord-014901-d9szap94 237 39 com com NN cord-014901-d9szap94 237 40 plexity plexity NN cord-014901-d9szap94 237 41 and and CC cord-014901-d9szap94 237 42 diversity diversity NN cord-014901-d9szap94 237 43 of of IN cord-014901-d9szap94 237 44 both both DT cord-014901-d9szap94 237 45 hosts host NNS cord-014901-d9szap94 237 46 and and CC cord-014901-d9szap94 237 47 pathogens pathogen NNS cord-014901-d9szap94 237 48 in in IN cord-014901-d9szap94 237 49 the the DT cord-014901-d9szap94 237 50 case case NN cord-014901-d9szap94 237 51 of of IN cord-014901-d9szap94 237 52 vaccines vaccine NNS cord-014901-d9szap94 237 53 for for IN cord-014901-d9szap94 237 54 animals animal NNS cord-014901-d9szap94 237 55 is be VBZ cord-014901-d9szap94 237 56 much much RB cord-014901-d9szap94 237 57 higher high JJR cord-014901-d9szap94 237 58 . . . cord-014901-d9szap94 238 1 Expression expression NN cord-014901-d9szap94 238 2 systems system NNS cord-014901-d9szap94 238 3 based base VBN cord-014901-d9szap94 238 4 on on IN cord-014901-d9szap94 238 5 the the DT cord-014901-d9szap94 238 6 use use NN cord-014901-d9szap94 238 7 of of IN cord-014901-d9szap94 238 8 plant plant NN cord-014901-d9szap94 238 9 cells cell NNS cord-014901-d9szap94 238 10 still still RB cord-014901-d9szap94 238 11 have have VBP cord-014901-d9szap94 238 12 a a DT cord-014901-d9szap94 238 13 limited limited JJ cord-014901-d9szap94 238 14 application application NN cord-014901-d9szap94 238 15 or or CC cord-014901-d9szap94 238 16 are be VBP cord-014901-d9szap94 238 17 used use VBN cord-014901-d9szap94 238 18 primarily primarily RB cord-014901-d9szap94 238 19 in in IN cord-014901-d9szap94 238 20 some some DT cord-014901-d9szap94 238 21 laboratories laboratory NNS cord-014901-d9szap94 238 22 . . . cord-014901-d9szap94 239 1 Nevertheless nevertheless RB cord-014901-d9szap94 239 2 , , , cord-014901-d9szap94 239 3 biopharming biopharming NN cord-014901-d9szap94 239 4 ( ( -LRB- cord-014901-d9szap94 239 5 the the DT cord-014901-d9szap94 239 6 biotechnological biotechnological JJ cord-014901-d9szap94 239 7 production production NN cord-014901-d9szap94 239 8 of of IN cord-014901-d9szap94 239 9 various various JJ cord-014901-d9szap94 239 10 substances substance NNS cord-014901-d9szap94 239 11 for for IN cord-014901-d9szap94 239 12 medicine medicine NN cord-014901-d9szap94 239 13 ) ) -RRB- cord-014901-d9szap94 239 14 in in IN cord-014901-d9szap94 239 15 plants plant NNS cord-014901-d9szap94 239 16 has have VBZ cord-014901-d9szap94 239 17 attracted attract VBN cord-014901-d9szap94 239 18 the the DT cord-014901-d9szap94 239 19 attention attention NN cord-014901-d9szap94 239 20 of of IN cord-014901-d9szap94 239 21 researchers researcher NNS cord-014901-d9szap94 239 22 and and CC cord-014901-d9szap94 239 23 manufacturers manufacturer NNS cord-014901-d9szap94 239 24 in in IN cord-014901-d9szap94 239 25 developed develop VBN cord-014901-d9szap94 239 26 countries country NNS cord-014901-d9szap94 239 27 . . . cord-014901-d9szap94 240 1 First first JJ cord-014901-d9szap94 240 2 biopharmaceuticals biopharmaceutical NNS cord-014901-d9szap94 240 3 of of IN cord-014901-d9szap94 240 4 plant plant NN cord-014901-d9szap94 240 5 origin origin NN cord-014901-d9szap94 240 6 , , , cord-014901-d9szap94 240 7 such such JJ cord-014901-d9szap94 240 8 as as IN cord-014901-d9szap94 240 9 anti anti JJ cord-014901-d9szap94 240 10 bodies body NNS cord-014901-d9szap94 240 11 ( ( -LRB- cord-014901-d9szap94 240 12 Anti anti JJ cord-014901-d9szap94 240 13 HBsAg HBsAg NNP cord-014901-d9szap94 240 14 required require VBD cord-014901-d9szap94 240 15 to to TO cord-014901-d9szap94 240 16 purify purify VB cord-014901-d9szap94 240 17 the the DT cord-014901-d9szap94 240 18 hepatitis hepatitis NN cord-014901-d9szap94 240 19 B B NNP cord-014901-d9szap94 240 20 vaccine vaccine NN cord-014901-d9szap94 240 21 ) ) -RRB- cord-014901-d9szap94 240 22 , , , cord-014901-d9szap94 240 23 therapeutic therapeutic JJ cord-014901-d9szap94 240 24 and and CC cord-014901-d9szap94 240 25 dietary dietary JJ cord-014901-d9szap94 240 26 proteins protein NNS cord-014901-d9szap94 240 27 ( ( -LRB- cord-014901-d9szap94 240 28 " " `` cord-014901-d9szap94 240 29 intrinsic intrinsic JJ cord-014901-d9szap94 240 30 factor factor NN cord-014901-d9szap94 240 31 " " '' cord-014901-d9szap94 240 32 required require VBN cord-014901-d9szap94 240 33 at at IN cord-014901-d9szap94 240 34 vitamin vitamin NN cord-014901-d9szap94 240 35 B12 B12 NNP cord-014901-d9szap94 240 36 deficiency deficiency NN cord-014901-d9szap94 240 37 , , , cord-014901-d9szap94 240 38 gastric gastric JJ cord-014901-d9szap94 240 39 lipase lipase NN cord-014901-d9szap94 240 40 ) ) -RRB- cord-014901-d9szap94 240 41 , , , cord-014901-d9szap94 240 42 have have VBP cord-014901-d9szap94 240 43 entered enter VBN cord-014901-d9szap94 240 44 the the DT cord-014901-d9szap94 240 45 market market NN cord-014901-d9szap94 240 46 , , , cord-014901-d9szap94 240 47 that that DT cord-014901-d9szap94 240 48 is be VBZ cord-014901-d9szap94 240 49 an an DT cord-014901-d9szap94 240 50 excellent excellent JJ cord-014901-d9szap94 240 51 illustration illustration NN cord-014901-d9szap94 240 52 of of IN cord-014901-d9szap94 240 53 this this DT cord-014901-d9szap94 240 54 progress progress NN cord-014901-d9szap94 240 55 [ [ -LRB- cord-014901-d9szap94 240 56 67 67 CD cord-014901-d9szap94 240 57 ] ] -RRB- cord-014901-d9szap94 240 58 . . . cord-014901-d9szap94 241 1 Despite despite IN cord-014901-d9szap94 241 2 the the DT cord-014901-d9szap94 241 3 fact fact NN cord-014901-d9szap94 241 4 that that IN cord-014901-d9szap94 241 5 today today NN cord-014901-d9szap94 241 6 the the DT cord-014901-d9szap94 241 7 number number NN cord-014901-d9szap94 241 8 of of IN cord-014901-d9szap94 241 9 biophar biophar NNP cord-014901-d9szap94 241 10 maceutical maceutical NNP cord-014901-d9szap94 241 11 proteins protein NNS cord-014901-d9szap94 241 12 expressed express VBN cord-014901-d9szap94 241 13 in in IN cord-014901-d9szap94 241 14 plant plant NN cord-014901-d9szap94 241 15 cells cell NNS cord-014901-d9szap94 241 16 is be VBZ cord-014901-d9szap94 241 17 enor enor NNP cord-014901-d9szap94 241 18 mous mous JJ cord-014901-d9szap94 241 19 , , , cord-014901-d9szap94 241 20 many many JJ cord-014901-d9szap94 241 21 questions question NNS cord-014901-d9szap94 241 22 still still RB cord-014901-d9szap94 241 23 remain remain VBP cord-014901-d9szap94 241 24 unresolved unresolved JJ cord-014901-d9szap94 241 25 . . . cord-014901-d9szap94 242 1 The the DT cord-014901-d9szap94 242 2 methods method NNS cord-014901-d9szap94 242 3 for for IN cord-014901-d9szap94 242 4 target target NN cord-014901-d9szap94 242 5 recombinant recombinant JJ cord-014901-d9szap94 242 6 protein protein NN cord-014901-d9szap94 242 7 quantification quantification NN cord-014901-d9szap94 242 8 and and CC cord-014901-d9szap94 242 9 purification purification NN cord-014901-d9szap94 242 10 are be VBP cord-014901-d9szap94 242 11 still still RB cord-014901-d9szap94 242 12 not not RB cord-014901-d9szap94 242 13 developed develop VBN cord-014901-d9szap94 242 14 for for IN cord-014901-d9szap94 242 15 most most JJS cord-014901-d9szap94 242 16 of of IN cord-014901-d9szap94 242 17 products product NNS cord-014901-d9szap94 242 18 . . . cord-014901-d9szap94 243 1 The the DT cord-014901-d9szap94 243 2 problems problem NNS cord-014901-d9szap94 243 3 of of IN cord-014901-d9szap94 243 4 transgene transgene NN cord-014901-d9szap94 243 5 silencing silencing NN cord-014901-d9szap94 243 6 and and CC cord-014901-d9szap94 243 7 increased increase VBN cord-014901-d9szap94 243 8 expression expression NN cord-014901-d9szap94 243 9 of of IN cord-014901-d9szap94 243 10 target target NN cord-014901-d9szap94 243 11 protein protein NN cord-014901-d9szap94 243 12 genes gene NNS cord-014901-d9szap94 243 13 are be VBP cord-014901-d9szap94 243 14 still still RB cord-014901-d9szap94 243 15 at at IN cord-014901-d9szap94 243 16 the the DT cord-014901-d9szap94 243 17 stage stage NN cord-014901-d9szap94 243 18 of of IN cord-014901-d9szap94 243 19 research research NN cord-014901-d9szap94 243 20 . . . cord-014901-d9szap94 244 1 The the DT cord-014901-d9szap94 244 2 important important JJ cord-014901-d9szap94 244 3 task task NN cord-014901-d9szap94 244 4 that that WDT cord-014901-d9szap94 244 5 has have VBZ cord-014901-d9szap94 244 6 yet yet RB cord-014901-d9szap94 244 7 to to TO cord-014901-d9szap94 244 8 be be VB cord-014901-d9szap94 244 9 solved solve VBN cord-014901-d9szap94 244 10 is be VBZ cord-014901-d9szap94 244 11 to to TO cord-014901-d9szap94 244 12 achieve achieve VB cord-014901-d9szap94 244 13 a a DT cord-014901-d9szap94 244 14 stable stable JJ cord-014901-d9szap94 244 15 level level NN cord-014901-d9szap94 244 16 of of IN cord-014901-d9szap94 244 17 expression expression NN cord-014901-d9szap94 244 18 in in IN cord-014901-d9szap94 244 19 different different JJ cord-014901-d9szap94 244 20 batches batch NNS cord-014901-d9szap94 244 21 of of IN cord-014901-d9szap94 244 22 plant plant NN cord-014901-d9szap94 244 23 raw raw JJ cord-014901-d9szap94 244 24 material material NN cord-014901-d9szap94 244 25 . . . cord-014901-d9szap94 245 1 Not not RB cord-014901-d9szap94 245 2 much much JJ cord-014901-d9szap94 245 3 work work NN cord-014901-d9szap94 245 4 appeared appear VBD cord-014901-d9szap94 245 5 for for IN cord-014901-d9szap94 245 6 judging judge VBG cord-014901-d9szap94 245 7 about about IN cord-014901-d9szap94 245 8 maintaining maintain VBG cord-014901-d9szap94 245 9 the the DT cord-014901-d9szap94 245 10 sta sta NNP cord-014901-d9szap94 245 11 bility bility NNP cord-014901-d9szap94 245 12 of of IN cord-014901-d9szap94 245 13 recombinant recombinant JJ cord-014901-d9szap94 245 14 proteins protein NNS cord-014901-d9szap94 245 15 after after IN cord-014901-d9szap94 245 16 the the DT cord-014901-d9szap94 245 17 harvest harvest NN cord-014901-d9szap94 245 18 , , , cord-014901-d9szap94 245 19 pro pro JJ cord-014901-d9szap94 245 20 cessing cessing NN cord-014901-d9szap94 245 21 , , , cord-014901-d9szap94 245 22 and and CC cord-014901-d9szap94 245 23 storage storage NN cord-014901-d9szap94 245 24 . . . cord-014901-d9szap94 246 1 All all DT cord-014901-d9szap94 246 2 of of IN cord-014901-d9szap94 246 3 these these DT cord-014901-d9szap94 246 4 problems problem NNS cord-014901-d9szap94 246 5 require require VBP cord-014901-d9szap94 246 6 additional additional JJ cord-014901-d9szap94 246 7 expenses expense NNS cord-014901-d9szap94 246 8 for for IN cord-014901-d9szap94 246 9 further further JJ cord-014901-d9szap94 246 10 research research NN cord-014901-d9szap94 246 11 . . . cord-014901-d9szap94 247 1 One one CD cord-014901-d9szap94 247 2 of of IN cord-014901-d9szap94 247 3 the the DT cord-014901-d9szap94 247 4 main main JJ cord-014901-d9szap94 247 5 obstacles obstacle NNS cord-014901-d9szap94 247 6 for for IN cord-014901-d9szap94 247 7 the the DT cord-014901-d9szap94 247 8 leading lead VBG cord-014901-d9szap94 247 9 research research NN cord-014901-d9szap94 247 10 groups group NNS cord-014901-d9szap94 247 11 working work VBG cord-014901-d9szap94 247 12 on on IN cord-014901-d9szap94 247 13 the the DT cord-014901-d9szap94 247 14 development development NN cord-014901-d9szap94 247 15 and and CC cord-014901-d9szap94 247 16 production production NN cord-014901-d9szap94 247 17 of of IN cord-014901-d9szap94 247 18 plant plant NN cord-014901-d9szap94 247 19 vaccines vaccine NNS cord-014901-d9szap94 247 20 , , , cord-014901-d9szap94 247 21 given give VBN cord-014901-d9szap94 247 22 the the DT cord-014901-d9szap94 247 23 financial financial JJ cord-014901-d9szap94 247 24 constraints constraint NNS cord-014901-d9szap94 247 25 , , , cord-014901-d9szap94 247 26 is be VBZ cord-014901-d9szap94 247 27 the the DT cord-014901-d9szap94 247 28 fulfillment fulfillment NN cord-014901-d9szap94 247 29 of of IN cord-014901-d9szap94 247 30 the the DT cord-014901-d9szap94 247 31 relevant relevant JJ cord-014901-d9szap94 247 32 official official JJ cord-014901-d9szap94 247 33 regulations regulation NNS cord-014901-d9szap94 247 34 govern govern VBP cord-014901-d9szap94 247 35 ing e VBG cord-014901-d9szap94 247 36 the the DT cord-014901-d9szap94 247 37 use use NN cord-014901-d9szap94 247 38 of of IN cord-014901-d9szap94 247 39 oral oral JJ cord-014901-d9szap94 247 40 medications medication NNS cord-014901-d9szap94 247 41 . . . cord-014901-d9szap94 248 1 To to IN cord-014901-d9szap94 248 2 date date NN cord-014901-d9szap94 248 3 , , , cord-014901-d9szap94 248 4 the the DT cord-014901-d9szap94 248 5 purified purify VBN cord-014901-d9szap94 248 6 vaccines vaccine NNS cord-014901-d9szap94 248 7 and and CC cord-014901-d9szap94 248 8 therapeutic therapeutic JJ cord-014901-d9szap94 248 9 proteins protein NNS cord-014901-d9szap94 248 10 of of IN cord-014901-d9szap94 248 11 plant plant NN cord-014901-d9szap94 248 12 origin origin NN cord-014901-d9szap94 248 13 must must MD cord-014901-d9szap94 248 14 meet meet VB cord-014901-d9szap94 248 15 the the DT cord-014901-d9szap94 248 16 same same JJ cord-014901-d9szap94 248 17 standards standard NNS cord-014901-d9szap94 248 18 relating relate VBG cord-014901-d9szap94 248 19 to to IN cord-014901-d9szap94 248 20 the the DT cord-014901-d9szap94 248 21 production production NN cord-014901-d9szap94 248 22 , , , cord-014901-d9szap94 248 23 biosafety biosafety NN cord-014901-d9szap94 248 24 , , , cord-014901-d9szap94 248 25 purification purification NN cord-014901-d9szap94 248 26 , , , cord-014901-d9szap94 248 27 storage storage NN cord-014901-d9szap94 248 28 , , , cord-014901-d9szap94 248 29 dosage dosage NN cord-014901-d9szap94 248 30 , , , cord-014901-d9szap94 248 31 etc etc FW cord-014901-d9szap94 248 32 . . . cord-014901-d9szap94 249 1 as as IN cord-014901-d9szap94 249 2 any any DT cord-014901-d9szap94 249 3 other other JJ cord-014901-d9szap94 249 4 recombinant recombinant JJ cord-014901-d9szap94 249 5 proteins protein NNS cord-014901-d9szap94 249 6 for for IN cord-014901-d9szap94 249 7 medical medical JJ cord-014901-d9szap94 249 8 purposes purpose NNS cord-014901-d9szap94 249 9 . . . cord-014901-d9szap94 250 1 Nevertheless nevertheless RB cord-014901-d9szap94 250 2 , , , cord-014901-d9szap94 250 3 despite despite IN cord-014901-d9szap94 250 4 these these DT cord-014901-d9szap94 250 5 difficulties difficulty NNS cord-014901-d9szap94 250 6 , , , cord-014901-d9szap94 250 7 there there EX cord-014901-d9szap94 250 8 were be VBD cord-014901-d9szap94 250 9 first first JJ cord-014901-d9szap94 250 10 biopharmaceuticals biopharmaceutical NNS cord-014901-d9szap94 250 11 of of IN cord-014901-d9szap94 250 12 plant plant NN cord-014901-d9szap94 250 13 origin origin NN cord-014901-d9szap94 250 14 that that WDT cord-014901-d9szap94 250 15 passed pass VBD cord-014901-d9szap94 250 16 all all PDT cord-014901-d9szap94 250 17 the the DT cord-014901-d9szap94 250 18 necessary necessary JJ cord-014901-d9szap94 250 19 tests test NNS cord-014901-d9szap94 250 20 and and CC cord-014901-d9szap94 250 21 were be VBD cord-014901-d9szap94 250 22 approved approve VBN cord-014901-d9szap94 250 23 for for IN cord-014901-d9szap94 250 24 use use NN cord-014901-d9szap94 250 25 by by IN cord-014901-d9szap94 250 26 the the DT cord-014901-d9szap94 250 27 relevant relevant JJ cord-014901-d9szap94 250 28 authorities authority NNS cord-014901-d9szap94 250 29 . . . cord-014901-d9szap94 251 1 Some some DT cord-014901-d9szap94 251 2 new new JJ cord-014901-d9szap94 251 3 products product NNS cord-014901-d9szap94 251 4 having have VBG cord-014901-d9szap94 251 5 spe spe NNP cord-014901-d9szap94 251 6 cific cific NNP cord-014901-d9szap94 251 7 advantages advantage NNS cord-014901-d9szap94 251 8 over over IN cord-014901-d9szap94 251 9 similar similar JJ cord-014901-d9szap94 251 10 products product NNS cord-014901-d9szap94 251 11 obtained obtain VBN cord-014901-d9szap94 251 12 in in IN cord-014901-d9szap94 251 13 mammalian mammalian JJ cord-014901-d9szap94 251 14 cell cell NN cord-014901-d9szap94 251 15 cultures culture NNS cord-014901-d9szap94 251 16 were be VBD cord-014901-d9szap94 251 17 developed develop VBN cord-014901-d9szap94 251 18 . . . cord-014901-d9szap94 252 1 Such such JJ cord-014901-d9szap94 252 2 com com NN cord-014901-d9szap94 252 3 panies panie NNS cord-014901-d9szap94 252 4 as as IN cord-014901-d9szap94 252 5 SemBioSys SemBioSys NNP cord-014901-d9szap94 252 6 Genetics Genetics NNP cord-014901-d9szap94 252 7 Inc. Inc. NNP cord-014901-d9szap94 252 8 ( ( -LRB- cord-014901-d9szap94 252 9 Calgary Calgary NNP cord-014901-d9szap94 252 10 , , , cord-014901-d9szap94 252 11 Can Can MD cord-014901-d9szap94 252 12 ada ada NNP cord-014901-d9szap94 252 13 ) ) -RRB- cord-014901-d9szap94 252 14 , , , cord-014901-d9szap94 252 15 Medicago Medicago NNP cord-014901-d9szap94 252 16 Inc. Inc. NNP cord-014901-d9szap94 252 17 ( ( -LRB- cord-014901-d9szap94 252 18 Quebec Quebec NNP cord-014901-d9szap94 252 19 , , , cord-014901-d9szap94 252 20 Canada Canada NNP cord-014901-d9szap94 252 21 ) ) -RRB- cord-014901-d9szap94 252 22 , , , cord-014901-d9szap94 252 23 Protalix Protalix NNP cord-014901-d9szap94 252 24 Bio Bio NNP cord-014901-d9szap94 252 25 Therapeutics Therapeutics NNP cord-014901-d9szap94 252 26 ( ( -LRB- cord-014901-d9szap94 252 27 Karmiel Karmiel NNP cord-014901-d9szap94 252 28 , , , cord-014901-d9szap94 252 29 Israel Israel NNP cord-014901-d9szap94 252 30 ) ) -RRB- cord-014901-d9szap94 252 31 , , , cord-014901-d9szap94 252 32 and and CC cord-014901-d9szap94 252 33 ORF ORF NNP cord-014901-d9szap94 252 34 Genetics Genetics NNP cord-014901-d9szap94 252 35 ( ( -LRB- cord-014901-d9szap94 252 36 Iceland Iceland NNP cord-014901-d9szap94 252 37 ) ) -RRB- cord-014901-d9szap94 252 38 proved prove VBD cord-014901-d9szap94 252 39 the the DT cord-014901-d9szap94 252 40 possibility possibility NN cord-014901-d9szap94 252 41 of of IN cord-014901-d9szap94 252 42 quick quick JJ cord-014901-d9szap94 252 43 establishing establishing NN cord-014901-d9szap94 252 44 of of IN cord-014901-d9szap94 252 45 the the DT cord-014901-d9szap94 252 46 production production NN cord-014901-d9szap94 252 47 of of IN cord-014901-d9szap94 252 48 purified purify VBN cord-014901-d9szap94 252 49 plant plant NN cord-014901-d9szap94 252 50 proteins protein NNS cord-014901-d9szap94 252 51 , , , cord-014901-d9szap94 252 52 which which WDT cord-014901-d9szap94 252 53 are be VBP cord-014901-d9szap94 252 54 quite quite RB cord-014901-d9szap94 252 55 competitive competitive JJ cord-014901-d9szap94 252 56 in in IN cord-014901-d9szap94 252 57 today today NN cord-014901-d9szap94 252 58 's 's POS cord-014901-d9szap94 252 59 market market NN cord-014901-d9szap94 252 60 . . . cord-014901-d9szap94 253 1 Progress progress NN cord-014901-d9szap94 253 2 has have VBZ cord-014901-d9szap94 253 3 been be VBN cord-014901-d9szap94 253 4 made make VBN cord-014901-d9szap94 253 5 in in IN cord-014901-d9szap94 253 6 the the DT cord-014901-d9szap94 253 7 formation formation NN cord-014901-d9szap94 253 8 of of IN cord-014901-d9szap94 253 9 the the DT cord-014901-d9szap94 253 10 legal legal JJ cord-014901-d9szap94 253 11 framework framework NN cord-014901-d9szap94 253 12 related relate VBN cord-014901-d9szap94 253 13 to to IN cord-014901-d9szap94 253 14 the the DT cord-014901-d9szap94 253 15 cultivation cultivation NN cord-014901-d9szap94 253 16 of of IN cord-014901-d9szap94 253 17 transgenic transgenic JJ cord-014901-d9szap94 253 18 plants plant NNS cord-014901-d9szap94 253 19 , , , cord-014901-d9szap94 253 20 testing testing NN cord-014901-d9szap94 253 21 and and CC cord-014901-d9szap94 253 22 use use NN cord-014901-d9szap94 253 23 of of IN cord-014901-d9szap94 253 24 plant plant NN cord-014901-d9szap94 253 25 biopharmaceuticals biopharmaceutical NNS cord-014901-d9szap94 253 26 . . . cord-014901-d9szap94 254 1 Several several JJ cord-014901-d9szap94 254 2 production production NN cord-014901-d9szap94 254 3 pro pro FW cord-014901-d9szap94 254 4 cesses cesse NNS cord-014901-d9szap94 254 5 based base VBN cord-014901-d9szap94 254 6 on on IN cord-014901-d9szap94 254 7 transgenic transgenic JJ cord-014901-d9szap94 254 8 plants plant NNS cord-014901-d9szap94 254 9 have have VBP cord-014901-d9szap94 254 10 already already RB cord-014901-d9szap94 254 11 received receive VBN cord-014901-d9szap94 254 12 a a DT cord-014901-d9szap94 254 13 brand brand NN cord-014901-d9szap94 254 14 GMP GMP NNP cord-014901-d9szap94 254 15 ( ( -LRB- cord-014901-d9szap94 254 16 Good Good NNP cord-014901-d9szap94 254 17 Manufacturing Manufacturing NNP cord-014901-d9szap94 254 18 Practice Practice NNP cord-014901-d9szap94 254 19 ) ) -RRB- cord-014901-d9szap94 254 20 , , , cord-014901-d9szap94 254 21 the the DT cord-014901-d9szap94 254 22 interest interest NN cord-014901-d9szap94 254 23 of of IN cord-014901-d9szap94 254 24 manufacturers manufacturer NNS cord-014901-d9szap94 254 25 to to IN cord-014901-d9szap94 254 26 this this DT cord-014901-d9szap94 254 27 field field NN cord-014901-d9szap94 254 28 of of IN cord-014901-d9szap94 254 29 biotechnology biotechnology NN cord-014901-d9szap94 254 30 began begin VBD cord-014901-d9szap94 254 31 to to TO cord-014901-d9szap94 254 32 increase increase VB cord-014901-d9szap94 254 33 again again RB cord-014901-d9szap94 254 34 . . . cord-014901-d9szap94 255 1 This this DT cord-014901-d9szap94 255 2 work work NN cord-014901-d9szap94 255 3 was be VBD cord-014901-d9szap94 255 4 performed perform VBN cord-014901-d9szap94 255 5 within within IN cord-014901-d9szap94 255 6 the the DT cord-014901-d9szap94 255 7 framework framework NN cord-014901-d9szap94 255 8 of of IN cord-014901-d9szap94 255 9 the the DT cord-014901-d9szap94 255 10 project project NN cord-014901-d9szap94 255 11 VI.62.1.5 VI.62.1.5 NNP cord-014901-d9szap94 256 1 ( ( -LRB- cord-014901-d9szap94 256 2 no no UH cord-014901-d9szap94 256 3 . . NN cord-014901-d9szap94 256 4 01201280334 01201280334 CD cord-014901-d9szap94 256 5 ) ) -RRB- cord-014901-d9szap94 257 1 Development development NN cord-014901-d9szap94 257 2 and and CC cord-014901-d9szap94 257 3 improvement improvement NN cord-014901-d9szap94 257 4 of of IN cord-014901-d9szap94 257 5 genetic genetic JJ cord-014901-d9szap94 257 6 constructs construct NNS cord-014901-d9szap94 257 7 to to TO cord-014901-d9szap94 257 8 optimize optimize VB cord-014901-d9szap94 257 9 the the DT cord-014901-d9szap94 257 10 expression expression NN cord-014901-d9szap94 257 11 of of IN cord-014901-d9szap94 257 12 target target NN cord-014901-d9szap94 257 13 genes gene NNS cord-014901-d9szap94 257 14 and and CC cord-014901-d9szap94 257 15 the the DT cord-014901-d9szap94 257 16 production production NN cord-014901-d9szap94 257 17 of of IN cord-014901-d9szap94 257 18 recombi recombi NNP cord-014901-d9szap94 257 19 nant nant NNP cord-014901-d9szap94 257 20 proteins protein NNS cord-014901-d9szap94 257 21 for for IN cord-014901-d9szap94 257 22 medical medical JJ cord-014901-d9szap94 257 23 purposes purpose NNS cord-014901-d9szap94 257 24 in in IN cord-014901-d9szap94 257 25 transgenic transgenic JJ cord-014901-d9szap94 257 26 plants plant NNS cord-014901-d9szap94 257 27 and and CC cord-014901-d9szap94 257 28 animals animal NNS cord-014901-d9szap94 257 29 . . . cord-014901-d9szap94 258 1 Plant plant NN cord-014901-d9szap94 258 2 produced produce VBD cord-014901-d9szap94 258 3 vaccines vaccine NNS cord-014901-d9szap94 258 4 : : : cord-014901-d9szap94 258 5 promise promise VB cord-014901-d9szap94 258 6 and and CC cord-014901-d9szap94 258 7 reality reality NN cord-014901-d9szap94 258 8 Evolution evolution NN cord-014901-d9szap94 258 9 of of IN cord-014901-d9szap94 258 10 plant plant NN cord-014901-d9szap94 258 11 made make VBD cord-014901-d9szap94 258 12 pharmaceu pharmaceu NNP cord-014901-d9szap94 258 13 ticals ticals NNP cord-014901-d9szap94 258 14 Current current JJ cord-014901-d9szap94 258 15 status status NN cord-014901-d9szap94 258 16 of of IN cord-014901-d9szap94 258 17 veterinary veterinary JJ cord-014901-d9szap94 258 18 vac vac NNP cord-014901-d9szap94 258 19 cines cines NNP cord-014901-d9szap94 258 20 Clinical Clinical NNP cord-014901-d9szap94 258 21 trials trial NNS cord-014901-d9szap94 258 22 fuel fuel VBP cord-014901-d9szap94 258 23 the the DT cord-014901-d9szap94 258 24 promise promise NN cord-014901-d9szap94 258 25 of of IN cord-014901-d9szap94 258 26 plant plant NN cord-014901-d9szap94 258 27 derived derive VBD cord-014901-d9szap94 258 28 vaccines vaccine NNS cord-014901-d9szap94 258 29 Expression expression NN cord-014901-d9szap94 258 30 of of IN cord-014901-d9szap94 258 31 hepatitis hepatitis NN cord-014901-d9szap94 258 32 B B NNP cord-014901-d9szap94 258 33 surface surface NN cord-014901-d9szap94 258 34 antigen antigen NN cord-014901-d9szap94 258 35 in in IN cord-014901-d9szap94 258 36 transgenic transgenic JJ cord-014901-d9szap94 258 37 plants plant NNS cord-014901-d9szap94 258 38 Oral oral JJ cord-014901-d9szap94 258 39 immunization immunization NN cord-014901-d9szap94 258 40 with with IN cord-014901-d9szap94 258 41 a a DT cord-014901-d9szap94 258 42 recombinant recombinant JJ cord-014901-d9szap94 258 43 bacterial bacterial JJ cord-014901-d9szap94 258 44 anti anti JJ cord-014901-d9szap94 258 45 gen gen NNP cord-014901-d9szap94 258 46 produced produce VBN cord-014901-d9szap94 258 47 in in IN cord-014901-d9szap94 258 48 transgenic transgenic JJ cord-014901-d9szap94 258 49 plants plant NNS cord-014901-d9szap94 258 50 Edible edible JJ cord-014901-d9szap94 258 51 vaccine vaccine NN cord-014901-d9szap94 258 52 protects protect VBZ cord-014901-d9szap94 258 53 mice mouse NNS cord-014901-d9szap94 258 54 against against IN cord-014901-d9szap94 258 55 Escher Escher NNP cord-014901-d9szap94 258 56 ichia ichia NNP cord-014901-d9szap94 258 57 coli coli NNS cord-014901-d9szap94 258 58 heat heat NN cord-014901-d9szap94 258 59 labile labile JJ cord-014901-d9szap94 258 60 enterotoxin enterotoxin NNP cord-014901-d9szap94 258 61 ( ( -LRB- cord-014901-d9szap94 258 62 LT LT NNP cord-014901-d9szap94 258 63 ) ) -RRB- cord-014901-d9szap94 258 64 : : : cord-014901-d9szap94 259 1 potatoes potato NNS cord-014901-d9szap94 259 2 express express VBP cord-014901-d9szap94 259 3 ing e VBG cord-014901-d9szap94 259 4 a a DT cord-014901-d9szap94 259 5 synthetic synthetic JJ cord-014901-d9szap94 259 6 LT LT NNP cord-014901-d9szap94 259 7 B B NNP cord-014901-d9szap94 259 8 gene gene NN cord-014901-d9szap94 259 9 Effi Effi NNP cord-014901-d9szap94 259 10 cacy cacy NN cord-014901-d9szap94 259 11 of of IN cord-014901-d9szap94 259 12 food food NN cord-014901-d9szap94 259 13 plant plant NN cord-014901-d9szap94 260 1 based base VBN cord-014901-d9szap94 260 2 oral oral JJ cord-014901-d9szap94 260 3 cholera cholera NN cord-014901-d9szap94 260 4 toxin toxin NN cord-014901-d9szap94 260 5 B B NNP cord-014901-d9szap94 260 6 subunit subunit NN cord-014901-d9szap94 260 7 vaccine vaccine NN cord-014901-d9szap94 260 8 Immunogenicity Immunogenicity NNP cord-014901-d9szap94 260 9 in in IN cord-014901-d9szap94 260 10 humans human NNS cord-014901-d9szap94 260 11 of of IN cord-014901-d9szap94 260 12 a a DT cord-014901-d9szap94 260 13 recombinant recombinant JJ cord-014901-d9szap94 260 14 bacte bacte JJ cord-014901-d9szap94 260 15 rial rial JJ cord-014901-d9szap94 260 16 antigen antigen NN cord-014901-d9szap94 260 17 delivered deliver VBN cord-014901-d9szap94 260 18 in in IN cord-014901-d9szap94 260 19 a a DT cord-014901-d9szap94 260 20 transgenic transgenic JJ cord-014901-d9szap94 260 21 potato potato NN cord-014901-d9szap94 260 22 Receptor receptor NN cord-014901-d9szap94 260 23 mediated mediate VBD cord-014901-d9szap94 260 24 oral oral JJ cord-014901-d9szap94 260 25 delivery delivery NN cord-014901-d9szap94 260 26 of of IN cord-014901-d9szap94 260 27 a a DT cord-014901-d9szap94 260 28 bioencapsulated bioencapsulated JJ cord-014901-d9szap94 260 29 green green JJ cord-014901-d9szap94 260 30 fluorescent fluorescent NN cord-014901-d9szap94 260 31 protein protein NN cord-014901-d9szap94 260 32 expressed express VBN cord-014901-d9szap94 260 33 in in IN cord-014901-d9szap94 260 34 transgenic transgenic JJ cord-014901-d9szap94 260 35 chlo chlo NN cord-014901-d9szap94 260 36 roplasts roplast NNS cord-014901-d9szap94 260 37 into into IN cord-014901-d9szap94 260 38 the the DT cord-014901-d9szap94 260 39 mouse mouse NN cord-014901-d9szap94 260 40 circulatory circulatory JJ cord-014901-d9szap94 260 41 system system NN cord-014901-d9szap94 260 42 Delivery delivery NN cord-014901-d9szap94 260 43 of of IN cord-014901-d9szap94 260 44 plant plant NN cord-014901-d9szap94 260 45 made make VBD cord-014901-d9szap94 260 46 vaccines vaccine NNS cord-014901-d9szap94 260 47 and and CC cord-014901-d9szap94 260 48 therapeutics therapeutic NNS cord-014901-d9szap94 260 49 Defending defend VBG cord-014901-d9szap94 260 50 the the DT cord-014901-d9szap94 260 51 mucosa mucosa NN cord-014901-d9szap94 260 52 : : : cord-014901-d9szap94 260 53 adjuvant adjuvant NN cord-014901-d9szap94 260 54 and and CC cord-014901-d9szap94 260 55 carrier carrier NN cord-014901-d9szap94 260 56 formula formula NN cord-014901-d9szap94 260 57 tions tion NNS cord-014901-d9szap94 260 58 for for IN cord-014901-d9szap94 260 59 mucosal mucosal JJ cord-014901-d9szap94 260 60 immunity immunity NN cord-014901-d9szap94 260 61 Induction Induction NNP cord-014901-d9szap94 260 62 of of IN cord-014901-d9szap94 260 63 secretory secretory JJ cord-014901-d9szap94 260 64 immunity immunity NN cord-014901-d9szap94 260 65 and and CC cord-014901-d9szap94 260 66 memory memory NN cord-014901-d9szap94 260 67 at at IN cord-014901-d9szap94 260 68 mucosal mucosal JJ cord-014901-d9szap94 260 69 surfaces surface NNS cord-014901-d9szap94 260 70 , , , cord-014901-d9szap94 260 71 Vaccine vaccine NN cord-014901-d9szap94 260 72 Oral oral JJ cord-014901-d9szap94 260 73 delivery delivery NN cord-014901-d9szap94 260 74 of of IN cord-014901-d9szap94 260 75 human human JJ cord-014901-d9szap94 260 76 biopharmaceuti biopharmaceuti NNP cord-014901-d9szap94 260 77 cals cals NNPS cord-014901-d9szap94 260 78 , , , cord-014901-d9szap94 260 79 autoantigens autoantigens NNP cord-014901-d9szap94 260 80 and and CC cord-014901-d9szap94 260 81 vaccine vaccine NN cord-014901-d9szap94 260 82 antigens antigen NNS cord-014901-d9szap94 260 83 bioencapsu bioencapsu NNP cord-014901-d9szap94 260 84 lated late VBN cord-014901-d9szap94 260 85 in in IN cord-014901-d9szap94 260 86 plant plant NN cord-014901-d9szap94 260 87 cells cell NNS cord-014901-d9szap94 261 1 The the DT cord-014901-d9szap94 261 2 mucosal mucosal JJ cord-014901-d9szap94 261 3 immune immune JJ cord-014901-d9szap94 261 4 response response NN cord-014901-d9szap94 261 5 to to IN cord-014901-d9szap94 261 6 plant plant NN cord-014901-d9szap94 261 7 derived derive VBN cord-014901-d9szap94 261 8 vaccines vaccine NNS cord-014901-d9szap94 261 9 Posttranslational Posttranslational NNP cord-014901-d9szap94 261 10 modifica modifica FW cord-014901-d9szap94 261 11 tion tion NN cord-014901-d9szap94 261 12 of of IN cord-014901-d9szap94 261 13 therapeutic therapeutic JJ cord-014901-d9szap94 261 14 proteins protein NNS cord-014901-d9szap94 261 15 in in IN cord-014901-d9szap94 261 16 plants plant NNS cord-014901-d9szap94 261 17 Seed Seed NNP cord-014901-d9szap94 261 18 based base VBN cord-014901-d9szap94 261 19 expression expression NN cord-014901-d9szap94 261 20 systems system NNS cord-014901-d9szap94 261 21 for for IN cord-014901-d9szap94 261 22 plant plant NN cord-014901-d9szap94 261 23 molecular molecular JJ cord-014901-d9szap94 261 24 farming farming NN cord-014901-d9szap94 262 1 The the DT cord-014901-d9szap94 262 2 economic economic JJ cord-014901-d9szap94 262 3 potential potential NN cord-014901-d9szap94 262 4 of of IN cord-014901-d9szap94 262 5 plant plant NN cord-014901-d9szap94 262 6 made make VBD cord-014901-d9szap94 262 7 pharmaceuticals pharmaceutical NNS cord-014901-d9szap94 262 8 in in IN cord-014901-d9szap94 262 9 the the DT cord-014901-d9szap94 262 10 manu manu NNP cord-014901-d9szap94 262 11 facture facture NNP cord-014901-d9szap94 262 12 of of IN cord-014901-d9szap94 262 13 biologic biologic JJ cord-014901-d9szap94 262 14 pharmaceuticals pharmaceutical NNS cord-014901-d9szap94 262 15 Rice Rice NNP cord-014901-d9szap94 262 16 based base VBD cord-014901-d9szap94 262 17 mucosal mucosal NNP cord-014901-d9szap94 262 18 vac vac NNP cord-014901-d9szap94 262 19 cine cine NNP cord-014901-d9szap94 262 20 as as IN cord-014901-d9szap94 262 21 a a DT cord-014901-d9szap94 262 22 global global JJ cord-014901-d9szap94 262 23 strategy strategy NN cord-014901-d9szap94 262 24 for for IN cord-014901-d9szap94 262 25 cold cold JJ cord-014901-d9szap94 262 26 chain chain NN cord-014901-d9szap94 262 27 and and CC cord-014901-d9szap94 262 28 needle needle NN cord-014901-d9szap94 262 29 free free JJ cord-014901-d9szap94 262 30 vaccination vaccination NN cord-014901-d9szap94 262 31 Secretory Secretory NNP cord-014901-d9szap94 262 32 IgA IgA NNP cord-014901-d9szap94 262 33 mediated mediate VBD cord-014901-d9szap94 262 34 protection protection NN cord-014901-d9szap94 262 35 against against IN cord-014901-d9szap94 262 36 V. V. NNP cord-014901-d9szap94 262 37 cholerae cholerae NN cord-014901-d9szap94 262 38 and and CC cord-014901-d9szap94 262 39 heat heat NN cord-014901-d9szap94 262 40 labile labile JJ cord-014901-d9szap94 262 41 enterotoxin enterotoxin JJ cord-014901-d9szap94 262 42 producing produce VBG cord-014901-d9szap94 262 43 enterotoxigenic enterotoxigenic JJ cord-014901-d9szap94 262 44 Escherichia Escherichia NNP cord-014901-d9szap94 262 45 coli coli NNS cord-014901-d9szap94 262 46 by by IN cord-014901-d9szap94 262 47 rice rice NN cord-014901-d9szap94 262 48 based base VBN cord-014901-d9szap94 262 49 vaccine vaccine NN cord-014901-d9szap94 262 50 Stability stability NN cord-014901-d9szap94 262 51 of of IN cord-014901-d9szap94 262 52 a a DT cord-014901-d9szap94 262 53 soybean soybean NN cord-014901-d9szap94 262 54 seed seed NN cord-014901-d9szap94 262 55 derived derive VBD cord-014901-d9szap94 262 56 vaccine vaccine NN cord-014901-d9szap94 262 57 antigen antigen NN cord-014901-d9szap94 262 58 following follow VBG cord-014901-d9szap94 262 59 long long JJ cord-014901-d9szap94 262 60 term term NN cord-014901-d9szap94 262 61 storage storage NN cord-014901-d9szap94 262 62 , , , cord-014901-d9szap94 262 63 processing processing NN cord-014901-d9szap94 262 64 and and CC cord-014901-d9szap94 262 65 transport transport NN cord-014901-d9szap94 262 66 in in IN cord-014901-d9szap94 262 67 the the DT cord-014901-d9szap94 262 68 absence absence NN cord-014901-d9szap94 262 69 of of IN cord-014901-d9szap94 262 70 a a DT cord-014901-d9szap94 262 71 cold cold JJ cord-014901-d9szap94 262 72 chain chain NN cord-014901-d9szap94 262 73 Tomato tomato NN cord-014901-d9szap94 262 74 is be VBZ cord-014901-d9szap94 262 75 a a DT cord-014901-d9szap94 262 76 highly highly RB cord-014901-d9szap94 262 77 effective effective JJ cord-014901-d9szap94 262 78 vehicle vehicle NN cord-014901-d9szap94 262 79 for for IN cord-014901-d9szap94 262 80 expression expression NN cord-014901-d9szap94 262 81 and and CC cord-014901-d9szap94 262 82 oral oral JJ cord-014901-d9szap94 262 83 immunization immunization NN cord-014901-d9szap94 262 84 with with IN cord-014901-d9szap94 262 85 Norwalk Norwalk NNP cord-014901-d9szap94 262 86 virus virus NN cord-014901-d9szap94 262 87 capsid capsid NN cord-014901-d9szap94 262 88 protein protein NN cord-014901-d9szap94 262 89 Production Production NNP cord-014901-d9szap94 262 90 of of IN cord-014901-d9szap94 262 91 plant plant NN cord-014901-d9szap94 262 92 made make VBD cord-014901-d9szap94 262 93 pharma pharma NNP cord-014901-d9szap94 262 94 ceuticals ceutical NNS cord-014901-d9szap94 262 95 : : : cord-014901-d9szap94 262 96 from from IN cord-014901-d9szap94 262 97 plant plant NN cord-014901-d9szap94 262 98 host host NN cord-014901-d9szap94 262 99 to to IN cord-014901-d9szap94 262 100 functional functional JJ cord-014901-d9szap94 262 101 protein protein NN cord-014901-d9szap94 262 102 Post Post NNP cord-014901-d9szap94 262 103 translational translational JJ cord-014901-d9szap94 262 104 modifica modifica FW cord-014901-d9szap94 262 105 tions tion NNS cord-014901-d9szap94 262 106 in in IN cord-014901-d9szap94 262 107 the the DT cord-014901-d9szap94 262 108 context context NN cord-014901-d9szap94 262 109 of of IN cord-014901-d9szap94 262 110 therapeutic therapeutic JJ cord-014901-d9szap94 262 111 proteins protein NNS cord-014901-d9szap94 263 1 Current current JJ cord-014901-d9szap94 263 2 achievements achievement NNS cord-014901-d9szap94 263 3 in in IN cord-014901-d9szap94 263 4 the the DT cord-014901-d9szap94 263 5 production production NN cord-014901-d9szap94 263 6 of of IN cord-014901-d9szap94 263 7 complex complex JJ cord-014901-d9szap94 263 8 biopharmaceuticals biopharmaceutical NNS cord-014901-d9szap94 263 9 with with IN cord-014901-d9szap94 263 10 moss moss NN cord-014901-d9szap94 263 11 bioreactors bioreactor NNS cord-014901-d9szap94 263 12 Low low RB cord-014901-d9szap94 263 13 dose dose JJ cord-014901-d9szap94 263 14 oral oral JJ cord-014901-d9szap94 263 15 immunization immunization NN cord-014901-d9szap94 263 16 with with IN cord-014901-d9szap94 263 17 lyophilized lyophilized JJ cord-014901-d9szap94 263 18 tissue tissue NN cord-014901-d9szap94 263 19 of of IN cord-014901-d9szap94 263 20 herbicide herbicide NN cord-014901-d9szap94 263 21 resistant resistant JJ cord-014901-d9szap94 263 22 lettuce lettuce NN cord-014901-d9szap94 263 23 expressing express VBG cord-014901-d9szap94 263 24 hepatitis hepatitis NNP cord-014901-d9szap94 263 25 B B NNP cord-014901-d9szap94 263 26 surface surface NN cord-014901-d9szap94 263 27 antigen antigen NN cord-014901-d9szap94 263 28 for for IN cord-014901-d9szap94 263 29 prototype prototype NN cord-014901-d9szap94 263 30 plant plant NN cord-014901-d9szap94 263 31 derived derive VBD cord-014901-d9szap94 263 32 vaccine vaccine NN cord-014901-d9szap94 263 33 tablet tablet NN cord-014901-d9szap94 263 34 formulation formulation NN cord-014901-d9szap94 263 35 RNA RNA NNP cord-014901-d9szap94 263 36 mediated mediate VBN cord-014901-d9szap94 263 37 chromatin chromatin NN cord-014901-d9szap94 263 38 based base VBN cord-014901-d9szap94 263 39 silencing silencing NN cord-014901-d9szap94 263 40 in in IN cord-014901-d9szap94 263 41 plants plant NNS cord-014901-d9szap94 263 42 Transcriptional Transcriptional NNP cord-014901-d9szap94 263 43 gene gene NN cord-014901-d9szap94 263 44 silencing silence VBG cord-014901-d9szap94 263 45 in in IN cord-014901-d9szap94 263 46 plants plant NNS cord-014901-d9szap94 263 47 Plant Plant NNP cord-014901-d9szap94 263 48 based base VBN cord-014901-d9szap94 263 49 production production NN cord-014901-d9szap94 263 50 of of IN cord-014901-d9szap94 263 51 biopharma biopharma NNP cord-014901-d9szap94 263 52 ceuticals ceuticals NNP cord-014901-d9szap94 263 53 Production Production NNP cord-014901-d9szap94 263 54 of of IN cord-014901-d9szap94 263 55 heterologous heterologous JJ cord-014901-d9szap94 263 56 proteins protein NNS cord-014901-d9szap94 263 57 in in IN cord-014901-d9szap94 263 58 plants plant NNS cord-014901-d9szap94 263 59 : : : cord-014901-d9szap94 263 60 strategies strategy NNS cord-014901-d9szap94 263 61 for for IN cord-014901-d9szap94 263 62 optimal optimal JJ cord-014901-d9szap94 263 63 expression expression NN cord-014901-d9szap94 263 64 Expression expression NN cord-014901-d9szap94 263 65 of of IN cord-014901-d9szap94 263 66 heterologous heterologous JJ cord-014901-d9szap94 263 67 genes gene NNS cord-014901-d9szap94 263 68 in in IN cord-014901-d9szap94 263 69 plant plant NN cord-014901-d9szap94 263 70 systems system NNS cord-014901-d9szap94 263 71 : : : cord-014901-d9szap94 263 72 new new JJ cord-014901-d9szap94 263 73 possibilities possibility NNS cord-014901-d9szap94 264 1 Exhaustion exhaustion NN cord-014901-d9szap94 264 2 of of IN cord-014901-d9szap94 264 3 the the DT cord-014901-d9szap94 264 4 chloroplast chloroplast NN cord-014901-d9szap94 264 5 protein protein NN cord-014901-d9szap94 264 6 synthesis synthesis NN cord-014901-d9szap94 264 7 capac capac NNP cord-014901-d9szap94 264 8 ity ity NNP cord-014901-d9szap94 264 9 by by IN cord-014901-d9szap94 264 10 massive massive JJ cord-014901-d9szap94 264 11 expression expression NN cord-014901-d9szap94 264 12 of of IN cord-014901-d9szap94 264 13 a a DT cord-014901-d9szap94 264 14 highly highly RB cord-014901-d9szap94 264 15 stable stable JJ cord-014901-d9szap94 264 16 protein protein NN cord-014901-d9szap94 264 17 anti anti AFX cord-014901-d9szap94 264 18 biotic biotic JJ cord-014901-d9szap94 264 19 Metabolic metabolic JJ cord-014901-d9szap94 264 20 adaptation adaptation NN cord-014901-d9szap94 264 21 in in IN cord-014901-d9szap94 264 22 transplastomic transplastomic JJ cord-014901-d9szap94 264 23 plants plant NNS cord-014901-d9szap94 264 24 massively massively RB cord-014901-d9szap94 264 25 accumu accumu JJ cord-014901-d9szap94 265 1 lating late VBG cord-014901-d9szap94 265 2 recombinant recombinant JJ cord-014901-d9szap94 265 3 proteins protein NNS cord-014901-d9szap94 265 4 Transplastomic Transplastomic NNP cord-014901-d9szap94 265 5 plants plant VBZ cord-014901-d9szap94 265 6 Overexpression Overexpression NNP cord-014901-d9szap94 265 7 of of IN cord-014901-d9szap94 265 8 the the DT cord-014901-d9szap94 265 9 Bt Bt NNP cord-014901-d9szap94 265 10 cry2Aa2 cry2Aa2 NNP cord-014901-d9szap94 265 11 operon operon NN cord-014901-d9szap94 266 1 a a DT cord-014901-d9szap94 266 2 ¸n´l ¸n´l NN cord-014901-d9szap94 266 3 in in IN cord-014901-d9szap94 266 4 chloroplasts chloroplast NNS cord-014901-d9szap94 266 5 leads lead VBZ cord-014901-d9szap94 266 6 to to IN cord-014901-d9szap94 266 7 formation formation NN cord-014901-d9szap94 266 8 of of IN cord-014901-d9szap94 266 9 insecticidal insecticidal JJ cord-014901-d9szap94 266 10 crys crys NNP cord-014901-d9szap94 266 11 tals tals NNPS cord-014901-d9szap94 266 12 Chloroplast Chloroplast NNP cord-014901-d9szap94 266 13 vector vector NN cord-014901-d9szap94 266 14 systems system NNS cord-014901-d9szap94 266 15 for for IN cord-014901-d9szap94 266 16 biotechnology biotechnology NN cord-014901-d9szap94 266 17 applications application NNS cord-014901-d9szap94 266 18 Determining determine VBG cord-014901-d9szap94 266 19 the the DT cord-014901-d9szap94 266 20 transgene transgene NN cord-014901-d9szap94 266 21 containment containment NN cord-014901-d9szap94 266 22 level level NN cord-014901-d9szap94 266 23 provided provide VBN cord-014901-d9szap94 266 24 by by IN cord-014901-d9szap94 266 25 chloroplast chloroplast NN cord-014901-d9szap94 266 26 transformation transformation NN cord-014901-d9szap94 266 27 Expression expression NN cord-014901-d9szap94 266 28 of of IN cord-014901-d9szap94 266 29 the the DT cord-014901-d9szap94 266 30 native native JJ cord-014901-d9szap94 266 31 cholera cholera NN cord-014901-d9szap94 266 32 toxin toxin NN cord-014901-d9szap94 266 33 B B NNP cord-014901-d9szap94 266 34 subunit subunit NN cord-014901-d9szap94 266 35 gene gene NN cord-014901-d9szap94 266 36 and and CC cord-014901-d9szap94 266 37 assembly assembly NN cord-014901-d9szap94 266 38 as as IN cord-014901-d9szap94 266 39 functional functional JJ cord-014901-d9szap94 266 40 oligomers oligomer NNS cord-014901-d9szap94 266 41 in in IN cord-014901-d9szap94 266 42 transgenic transgenic JJ cord-014901-d9szap94 266 43 tobacco tobacco NN cord-014901-d9szap94 266 44 chloroplasts chloroplast NNS cord-014901-d9szap94 266 45 Chloroplast chloroplast JJ cord-014901-d9szap94 266 46 expression expression NN cord-014901-d9szap94 266 47 of of IN cord-014901-d9szap94 266 48 His -PRON- PRP$ cord-014901-d9szap94 266 49 tagged tag VBN cord-014901-d9szap94 266 50 GUS GUS NNP cord-014901-d9szap94 266 51 fusions fusion NNS cord-014901-d9szap94 266 52 : : : cord-014901-d9szap94 266 53 a a DT cord-014901-d9szap94 266 54 general general JJ cord-014901-d9szap94 266 55 strategy strategy NN cord-014901-d9szap94 266 56 to to IN cord-014901-d9szap94 266 57 over over IN cord-014901-d9szap94 266 58 produce produce NN cord-014901-d9szap94 266 59 and and CC cord-014901-d9szap94 266 60 purify purify VB cord-014901-d9szap94 266 61 foreign foreign JJ cord-014901-d9szap94 266 62 proteins protein NNS cord-014901-d9szap94 266 63 using use VBG cord-014901-d9szap94 266 64 transplas transplas NNP cord-014901-d9szap94 266 65 tomic tomic NNP cord-014901-d9szap94 266 66 plants plant NNS cord-014901-d9szap94 266 67 as as IN cord-014901-d9szap94 266 68 bioreactors bioreactor NNS cord-014901-d9szap94 266 69 Accumulation Accumulation NNP cord-014901-d9szap94 266 70 of of IN cord-014901-d9szap94 266 71 trehalose trehalose DT cord-014901-d9szap94 266 72 within within IN cord-014901-d9szap94 266 73 transgenic transgenic JJ cord-014901-d9szap94 266 74 chloro chloro NN cord-014901-d9szap94 266 75 plasts plast NNS cord-014901-d9szap94 266 76 confers confer VBZ cord-014901-d9szap94 266 77 drought drought NN cord-014901-d9szap94 266 78 tolerance tolerance NN cord-014901-d9szap94 266 79 Plants plant NNS cord-014901-d9szap94 266 80 as as IN cord-014901-d9szap94 266 81 bioreactors bioreactor NNS cord-014901-d9szap94 266 82 for for IN cord-014901-d9szap94 266 83 the the DT cord-014901-d9szap94 266 84 production production NN cord-014901-d9szap94 266 85 of of IN cord-014901-d9szap94 266 86 vaccine vaccine NN cord-014901-d9szap94 266 87 anti anti JJ cord-014901-d9szap94 266 88 gens gen NNS cord-014901-d9szap94 266 89 Towards towards IN cord-014901-d9szap94 266 90 high high JJ cord-014901-d9szap94 266 91 yield yield NN cord-014901-d9szap94 266 92 production production NN cord-014901-d9szap94 266 93 of of IN cord-014901-d9szap94 266 94 pharmaceutical pharmaceutical JJ cord-014901-d9szap94 266 95 proteins protein NNS cord-014901-d9szap94 266 96 with with IN cord-014901-d9szap94 266 97 plant plant NN cord-014901-d9szap94 266 98 cell cell NN cord-014901-d9szap94 266 99 suspension suspension NN cord-014901-d9szap94 266 100 cultures culture NNS cord-014901-d9szap94 266 101 Position position VB cord-014901-d9szap94 266 102 effects effect NNS cord-014901-d9szap94 266 103 and and CC cord-014901-d9szap94 266 104 epigenetic epigenetic JJ cord-014901-d9szap94 266 105 silencing silencing NN cord-014901-d9szap94 266 106 of of IN cord-014901-d9szap94 266 107 plant plant NN cord-014901-d9szap94 266 108 transgenes transgene VBN cord-014901-d9szap94 266 109 Bioreactor Bioreactor NNP cord-014901-d9szap94 266 110 systems system NNS cord-014901-d9szap94 266 111 for for IN cord-014901-d9szap94 266 112 in in FW cord-014901-d9szap94 266 113 vitro vitro FW cord-014901-d9szap94 266 114 production production NN cord-014901-d9szap94 266 115 of of IN cord-014901-d9szap94 266 116 foreign foreign JJ cord-014901-d9szap94 266 117 proteins protein NNS cord-014901-d9szap94 266 118 using use VBG cord-014901-d9szap94 266 119 plant plant NN cord-014901-d9szap94 266 120 cell cell NN cord-014901-d9szap94 266 121 cultures culture NNS cord-014901-d9szap94 266 122 High high JJ cord-014901-d9szap94 266 123 expression expression NN cord-014901-d9szap94 266 124 of of IN cord-014901-d9szap94 266 125 transgene transgene NN cord-014901-d9szap94 266 126 protein protein NN cord-014901-d9szap94 266 127 in in IN cord-014901-d9szap94 266 128 Spirodela Spirodela NNP cord-014901-d9szap94 266 129 Micro Micro NNP cord-014901-d9szap94 266 130 algae algae NN cord-014901-d9szap94 267 1 come come VB cord-014901-d9szap94 267 2 of of IN cord-014901-d9szap94 267 3 age age NN cord-014901-d9szap94 267 4 as as IN cord-014901-d9szap94 267 5 a a DT cord-014901-d9szap94 267 6 platform platform NN cord-014901-d9szap94 267 7 for for IN cord-014901-d9szap94 267 8 recombinant recombinant JJ cord-014901-d9szap94 267 9 protein protein NN cord-014901-d9szap94 267 10 production production NN cord-014901-d9szap94 267 11 Foot Foot NNP cord-014901-d9szap94 267 12 and and CC cord-014901-d9szap94 267 13 mouth mouth NN cord-014901-d9szap94 267 14 disease disease NN cord-014901-d9szap94 267 15 virus virus NN cord-014901-d9szap94 267 16 VP1 VP1 NNP cord-014901-d9szap94 267 17 pro pro NNP cord-014901-d9szap94 267 18 tein tein NNP cord-014901-d9szap94 267 19 fused fuse VBD cord-014901-d9szap94 267 20 with with IN cord-014901-d9szap94 267 21 cholera cholera NN cord-014901-d9szap94 267 22 toxin toxin NN cord-014901-d9szap94 267 23 B B NNP cord-014901-d9szap94 267 24 subunit subunit NN cord-014901-d9szap94 267 25 expressed express VBD cord-014901-d9szap94 267 26 in in IN cord-014901-d9szap94 267 27 Chlamydomonas Chlamydomonas NNP cord-014901-d9szap94 267 28 reinhardtii reinhardtii NN cord-014901-d9szap94 267 29 chloroplast chloroplast NN cord-014901-d9szap94 267 30 Heat Heat NNP cord-014901-d9szap94 267 31 stable stable JJ cord-014901-d9szap94 267 32 oral oral JJ cord-014901-d9szap94 267 33 alga alga NN cord-014901-d9szap94 267 34 based base VBN cord-014901-d9szap94 267 35 vaccine vaccine NN cord-014901-d9szap94 267 36 pro pro JJ cord-014901-d9szap94 267 37 tects tect NNS cord-014901-d9szap94 267 38 mice mouse NNS cord-014901-d9szap94 267 39 from from IN cord-014901-d9szap94 267 40 Staphylococcus Staphylococcus NNP cord-014901-d9szap94 267 41 aureus aureus NN cord-014901-d9szap94 267 42 infection infection NN cord-014901-d9szap94 267 43 Factors factor NNS cord-014901-d9szap94 267 44 effecting effect VBG cord-014901-d9szap94 267 45 expression expression NN cord-014901-d9szap94 267 46 of of IN cord-014901-d9szap94 267 47 vaccines vaccine NNS cord-014901-d9szap94 267 48 in in IN cord-014901-d9szap94 267 49 microalgae microalgae JJ cord-014901-d9szap94 267 50 Recombination recombination NN cord-014901-d9szap94 267 51 and and CC cord-014901-d9szap94 267 52 expression expression NN cord-014901-d9szap94 267 53 of of IN cord-014901-d9szap94 267 54 classical classical JJ cord-014901-d9szap94 267 55 swine swine NN cord-014901-d9szap94 267 56 fever fever NN cord-014901-d9szap94 267 57 virus virus NN cord-014901-d9szap94 267 58 ( ( -LRB- cord-014901-d9szap94 267 59 CSFV CSFV NNP cord-014901-d9szap94 267 60 ) ) -RRB- cord-014901-d9szap94 267 61 structural structural JJ cord-014901-d9szap94 267 62 protein protein NN cord-014901-d9szap94 267 63 E2 E2 NNP cord-014901-d9szap94 267 64 gene gene NN cord-014901-d9szap94 267 65 in in IN cord-014901-d9szap94 267 66 Chlamydomonas Chlamydomonas NNP cord-014901-d9szap94 267 67 rein rein NNP cord-014901-d9szap94 267 68 hardtii hardtii NNP cord-014901-d9szap94 268 1 chroloplasts chroloplasts NNP cord-014901-d9szap94 269 1 Viral viral JJ cord-014901-d9szap94 269 2 vec vec NN cord-014901-d9szap94 269 3 tors tor NNS cord-014901-d9szap94 269 4 for for IN cord-014901-d9szap94 269 5 the the DT cord-014901-d9szap94 269 6 expression expression NN cord-014901-d9szap94 269 7 of of IN cord-014901-d9szap94 269 8 proteins protein NNS cord-014901-d9szap94 269 9 in in IN cord-014901-d9szap94 269 10 plants plant NNS cord-014901-d9szap94 270 1 Agrobacterium agrobacterium NN cord-014901-d9szap94 270 2 mediated mediate VBD cord-014901-d9szap94 270 3 transient transient JJ cord-014901-d9szap94 270 4 expression expression NN cord-014901-d9szap94 270 5 as as IN cord-014901-d9szap94 270 6 an an DT cord-014901-d9szap94 270 7 approach approach NN cord-014901-d9szap94 270 8 to to IN cord-014901-d9szap94 270 9 production production NN cord-014901-d9szap94 270 10 of of IN cord-014901-d9szap94 270 11 recombi recombi NNP cord-014901-d9szap94 270 12 nant nant NNP cord-014901-d9szap94 270 13 proteins protein NNS cord-014901-d9szap94 270 14 in in IN cord-014901-d9szap94 270 15 plants plant NNS cord-014901-d9szap94 271 1 A a DT cord-014901-d9szap94 271 2 novel novel JJ cord-014901-d9szap94 271 3 two two CD cord-014901-d9szap94 271 4 component component NN cord-014901-d9szap94 271 5 tobacco tobacco NN cord-014901-d9szap94 271 6 mosaic mosaic NNP cord-014901-d9szap94 271 7 virus virus NN cord-014901-d9szap94 271 8 based base VBN cord-014901-d9szap94 271 9 vector vector NN cord-014901-d9szap94 271 10 system system NN cord-014901-d9szap94 271 11 for for IN cord-014901-d9szap94 271 12 high high JJ cord-014901-d9szap94 271 13 level level NN cord-014901-d9szap94 271 14 expression expression NN cord-014901-d9szap94 271 15 of of IN cord-014901-d9szap94 271 16 multiple multiple JJ cord-014901-d9szap94 271 17 ther ther NNP cord-014901-d9szap94 271 18 apeutic apeutic JJ cord-014901-d9szap94 271 19 proteins protein NNS cord-014901-d9szap94 271 20 including include VBG cord-014901-d9szap94 271 21 a a DT cord-014901-d9szap94 271 22 human human JJ cord-014901-d9szap94 271 23 monoclonal monoclonal JJ cord-014901-d9szap94 271 24 anti anti JJ cord-014901-d9szap94 271 25 body body NN cord-014901-d9szap94 271 26 in in IN cord-014901-d9szap94 271 27 plants plant NNS cord-014901-d9szap94 272 1 Magnifec Magnifec NNP cord-014901-d9szap94 272 2 tion tion NN cord-014901-d9szap94 272 3 -a -a HYPH cord-014901-d9szap94 272 4 new new JJ cord-014901-d9szap94 272 5 platform platform NN cord-014901-d9szap94 272 6 for for IN cord-014901-d9szap94 272 7 expressing express VBG cord-014901-d9szap94 272 8 recombinant recombinant JJ cord-014901-d9szap94 272 9 vac vac NNP cord-014901-d9szap94 272 10 cines cines NNP cord-014901-d9szap94 272 11 in in IN cord-014901-d9szap94 272 12 plants plant NNS cord-014901-d9szap94 272 13 Plant Plant NNP cord-014901-d9szap94 272 14 based base VBD cord-014901-d9szap94 272 15 vaccines vaccine NNS cord-014901-d9szap94 272 16 : : : cord-014901-d9szap94 272 17 unique unique JJ cord-014901-d9szap94 272 18 advan advan JJ cord-014901-d9szap94 272 19 tages tage NNS cord-014901-d9szap94 272 20 Expression expression NN cord-014901-d9szap94 272 21 of of IN cord-014901-d9szap94 272 22 the the DT cord-014901-d9szap94 272 23 Newcastle Newcastle NNP cord-014901-d9szap94 272 24 disease disease NN cord-014901-d9szap94 272 25 virus virus NN cord-014901-d9szap94 272 26 fusion fusion NN cord-014901-d9szap94 272 27 protein protein NN cord-014901-d9szap94 272 28 in in IN cord-014901-d9szap94 272 29 transgenic transgenic JJ cord-014901-d9szap94 272 30 maize maize NN cord-014901-d9szap94 272 31 and and CC cord-014901-d9szap94 272 32 immunological immunological JJ cord-014901-d9szap94 272 33 studies study NNS cord-014901-d9szap94 272 34 Multimerization Multimerization NNP cord-014901-d9szap94 272 35 of of IN cord-014901-d9szap94 272 36 peptide peptide NN cord-014901-d9szap94 272 37 antigens antigen NNS cord-014901-d9szap94 272 38 for for IN cord-014901-d9szap94 272 39 pro pro JJ cord-014901-d9szap94 272 40 duction duction NN cord-014901-d9szap94 272 41 of of IN cord-014901-d9szap94 272 42 stable stable JJ cord-014901-d9szap94 272 43 immunogens immunogen NNS cord-014901-d9szap94 272 44 in in IN cord-014901-d9szap94 272 45 transgenic transgenic JJ cord-014901-d9szap94 272 46 plants plant NNS cord-014901-d9szap94 272 47 Generation generation NN cord-014901-d9szap94 272 48 and and CC cord-014901-d9szap94 272 49 immunogenicity immunogenicity NN cord-014901-d9szap94 272 50 of of IN cord-014901-d9szap94 272 51 Japanese japanese JJ cord-014901-d9szap94 272 52 encepha encepha NNP cord-014901-d9szap94 272 53 litis litis NNP cord-014901-d9szap94 272 54 virus virus NN cord-014901-d9szap94 272 55 envelope envelope NN cord-014901-d9szap94 272 56 protein protein NN cord-014901-d9szap94 272 57 expressed express VBN cord-014901-d9szap94 272 58 in in IN cord-014901-d9szap94 272 59 transgenic transgenic JJ cord-014901-d9szap94 272 60 rice rice NN cord-014901-d9szap94 272 61 Induction Induction NNP cord-014901-d9szap94 272 62 of of IN cord-014901-d9szap94 272 63 protective protective JJ cord-014901-d9szap94 272 64 immunity immunity NN cord-014901-d9szap94 272 65 in in IN cord-014901-d9szap94 272 66 swine swine NN cord-014901-d9szap94 272 67 by by IN cord-014901-d9szap94 272 68 recombinant recombinant JJ cord-014901-d9szap94 272 69 bamboo bamboo NN cord-014901-d9szap94 272 70 mosaic mosaic NNP cord-014901-d9szap94 272 71 virus virus NN cord-014901-d9szap94 272 72 express express VBP cord-014901-d9szap94 272 73 ing ing NNP cord-014901-d9szap94 272 74 foot foot NN cord-014901-d9szap94 272 75 and and CC cord-014901-d9szap94 272 76 mouth mouth NN cord-014901-d9szap94 272 77 disease disease NN cord-014901-d9szap94 272 78 virus virus NN cord-014901-d9szap94 272 79 epitopes epitopes NNP cord-014901-d9szap94 273 1 In in IN cord-014901-d9szap94 273 2 planta planta NN cord-014901-d9szap94 273 3 production production NN cord-014901-d9szap94 273 4 of of IN cord-014901-d9szap94 273 5 two two CD cord-014901-d9szap94 273 6 peptides peptide NNS cord-014901-d9szap94 273 7 of of IN cord-014901-d9szap94 273 8 the the DT cord-014901-d9szap94 273 9 classical classical JJ cord-014901-d9szap94 273 10 swine swine NN cord-014901-d9szap94 273 11 fever fever NN cord-014901-d9szap94 273 12 virus virus NN cord-014901-d9szap94 273 13 ( ( -LRB- cord-014901-d9szap94 273 14 CSFV CSFV NNP cord-014901-d9szap94 273 15 ) ) -RRB- cord-014901-d9szap94 273 16 E2 E2 NNP cord-014901-d9szap94 273 17 glycoprotein glycoprotein NN cord-014901-d9szap94 273 18 fused fuse VBD cord-014901-d9szap94 273 19 to to IN cord-014901-d9szap94 273 20 the the DT cord-014901-d9szap94 273 21 coat coat NN cord-014901-d9szap94 273 22 protein protein NN cord-014901-d9szap94 273 23 of of IN cord-014901-d9szap94 273 24 potato potato NN cord-014901-d9szap94 273 25 virus virus NN cord-014901-d9szap94 273 26 X x NN cord-014901-d9szap94 273 27 Expression expression NN cord-014901-d9szap94 273 28 in in IN cord-014901-d9szap94 273 29 plants plant NNS cord-014901-d9szap94 273 30 and and CC cord-014901-d9szap94 273 31 immunogenicity immunogenicity NN cord-014901-d9szap94 273 32 of of IN cord-014901-d9szap94 273 33 plant plant NN cord-014901-d9szap94 273 34 virus virus NN cord-014901-d9szap94 273 35 based base VBN cord-014901-d9szap94 273 36 exper exper NNP cord-014901-d9szap94 273 37 imental imental NNP cord-014901-d9szap94 273 38 rabies rabies NNP cord-014901-d9szap94 273 39 vaccine vaccine NN cord-014901-d9szap94 273 40 Delivery Delivery NNP cord-014901-d9szap94 273 41 of of IN cord-014901-d9szap94 273 42 subunit subunit NN cord-014901-d9szap94 273 43 vaccines vaccine NNS cord-014901-d9szap94 273 44 in in IN cord-014901-d9szap94 273 45 maize maize NN cord-014901-d9szap94 273 46 seed seed NN cord-014901-d9szap94 273 47 A a DT cord-014901-d9szap94 273 48 corn corn NN cord-014901-d9szap94 273 49 based base VBN cord-014901-d9szap94 273 50 deliv deliv NN cord-014901-d9szap94 273 51 ery ery JJ cord-014901-d9szap94 273 52 system system NN cord-014901-d9szap94 273 53 for for IN cord-014901-d9szap94 273 54 animal animal NN cord-014901-d9szap94 273 55 vaccines vaccine NNS cord-014901-d9szap94 273 56 : : : cord-014901-d9szap94 273 57 an an DT cord-014901-d9szap94 273 58 oral oral JJ cord-014901-d9szap94 273 59 transmissible transmissible JJ cord-014901-d9szap94 273 60 gastroenteritis gastroenteritis JJ cord-014901-d9szap94 273 61 virus virus NN cord-014901-d9szap94 273 62 vaccine vaccine NN cord-014901-d9szap94 273 63 boosts boost VBZ cord-014901-d9szap94 273 64 lactogenic lactogenic NNP cord-014901-d9szap94 273 65 immu immu NNP cord-014901-d9szap94 273 66 nity nity NNP cord-014901-d9szap94 273 67 in in IN cord-014901-d9szap94 273 68 swine swine NN cord-014901-d9szap94 273 69 Immunogenicity Immunogenicity NNP cord-014901-d9szap94 273 70 of of IN cord-014901-d9szap94 273 71 porcine porcine JJ cord-014901-d9szap94 273 72 transmissible transmissible JJ cord-014901-d9szap94 273 73 gastroenteritis gastroenteritis NN cord-014901-d9szap94 273 74 virus virus NN cord-014901-d9szap94 273 75 spike spike NN cord-014901-d9szap94 273 76 protein protein NN cord-014901-d9szap94 273 77 expressed express VBD cord-014901-d9szap94 273 78 in in IN cord-014901-d9szap94 273 79 plants plant NNS cord-014901-d9szap94 273 80 Oral oral JJ cord-014901-d9szap94 273 81 immunization immunization NN cord-014901-d9szap94 273 82 using use VBG cord-014901-d9szap94 273 83 tuber tuber NN cord-014901-d9szap94 273 84 extracts extract NNS cord-014901-d9szap94 273 85 from from IN cord-014901-d9szap94 273 86 transgenic transgenic JJ cord-014901-d9szap94 273 87 potato potato NN cord-014901-d9szap94 273 88 plants plant NNS cord-014901-d9szap94 273 89 expressing express VBG cord-014901-d9szap94 273 90 rabbit rabbit NN cord-014901-d9szap94 273 91 hemorrhagic hemorrhagic JJ cord-014901-d9szap94 273 92 disease disease NN cord-014901-d9szap94 273 93 virus virus NN cord-014901-d9szap94 273 94 capsid capsid NN cord-014901-d9szap94 273 95 protein protein NN cord-014901-d9szap94 273 96 Pea Pea NNP cord-014901-d9szap94 273 97 derived derive VBD cord-014901-d9szap94 273 98 vaccines vaccine NNS cord-014901-d9szap94 273 99 demonstrate demonstrate VBP cord-014901-d9szap94 273 100 high high JJ cord-014901-d9szap94 273 101 immunoge immunoge NNP cord-014901-d9szap94 273 102 nicity nicity NN cord-014901-d9szap94 273 103 and and CC cord-014901-d9szap94 273 104 protection protection NN cord-014901-d9szap94 273 105 in in IN cord-014901-d9szap94 273 106 rabbits rabbit NNS cord-014901-d9szap94 273 107 against against IN cord-014901-d9szap94 273 108 rabbit rabbit NN cord-014901-d9szap94 273 109 haemor haemor NNP cord-014901-d9szap94 273 110 rhagic rhagic NNP cord-014901-d9szap94 273 111 disease disease NNP cord-014901-d9szap94 273 112 virus virus NN cord-014901-d9szap94 273 113 Plant Plant NNP cord-014901-d9szap94 273 114 made make VBD cord-014901-d9szap94 273 115 pharmaceuti pharmaceuti NNP cord-014901-d9szap94 273 116 cals cal NNS cord-014901-d9szap94 273 117 : : : cord-014901-d9szap94 273 118 leading lead VBG cord-014901-d9szap94 273 119 products product NNS cord-014901-d9szap94 273 120 and and CC cord-014901-d9szap94 273 121 production production NN cord-014901-d9szap94 273 122 platforms platform NNS cord-014901-d9szap94 273 123 Pro pro JJ cord-014901-d9szap94 273 124 duction duction NN cord-014901-d9szap94 273 125 of of IN cord-014901-d9szap94 273 126 immunogenic immunogenic JJ cord-014901-d9szap94 273 127 VP6 vp6 NN cord-014901-d9szap94 273 128 protein protein NN cord-014901-d9szap94 273 129 of of IN cord-014901-d9szap94 273 130 bovine bovine JJ cord-014901-d9szap94 273 131 group group NN cord-014901-d9szap94 273 132 A a DT cord-014901-d9szap94 273 133 rotavirus rotavirus NN cord-014901-d9szap94 273 134 in in IN cord-014901-d9szap94 273 135 transgenic transgenic JJ cord-014901-d9szap94 273 136 potato potato NN cord-014901-d9szap94 273 137 plants plant NNS cord-014901-d9szap94 274 1 Rotavirus rotavirus NN cord-014901-d9szap94 274 2 VP6 VP6 NNS cord-014901-d9szap94 274 3 expressed express VBN cord-014901-d9szap94 274 4 by by IN cord-014901-d9szap94 274 5 PVX PVX NNP cord-014901-d9szap94 274 6 vectors vector NNS cord-014901-d9szap94 274 7 in in IN cord-014901-d9szap94 274 8 Nicotiana Nicotiana NNP cord-014901-d9szap94 274 9 benthamiana benthamiana NNP cord-014901-d9szap94 274 10 coats coat NNS cord-014901-d9szap94 274 11 PVX PVX NNP cord-014901-d9szap94 274 12 rods rod NNS cord-014901-d9szap94 274 13 and and CC cord-014901-d9szap94 274 14 also also RB cord-014901-d9szap94 274 15 assembles assemble VBZ cord-014901-d9szap94 274 16 into into IN cord-014901-d9szap94 274 17 viruslike viruslike JJ cord-014901-d9szap94 274 18 parti parti FW cord-014901-d9szap94 274 19 cles cles FW cord-014901-d9szap94 274 20 Expression Expression NNP cord-014901-d9szap94 274 21 of of IN cord-014901-d9szap94 274 22 rotavirus rotavirus JJ cord-014901-d9szap94 274 23 capsid capsid NN cord-014901-d9szap94 274 24 protein protein NN cord-014901-d9szap94 274 25 VP6 VP6 NNS cord-014901-d9szap94 274 26 in in IN cord-014901-d9szap94 274 27 transgenic transgenic JJ cord-014901-d9szap94 274 28 potato potato NN cord-014901-d9szap94 274 29 and and CC cord-014901-d9szap94 274 30 its -PRON- PRP$ cord-014901-d9szap94 274 31 oral oral JJ cord-014901-d9szap94 274 32 immuno immuno JJ cord-014901-d9szap94 274 33 genicity genicity NN cord-014901-d9szap94 274 34 in in IN cord-014901-d9szap94 274 35 mice mouse NNS cord-014901-d9szap94 274 36 Protective protective JJ cord-014901-d9szap94 274 37 lactogenic lactogenic JJ cord-014901-d9szap94 274 38 immunity immunity NN cord-014901-d9szap94 274 39 conferred confer VBN cord-014901-d9szap94 274 40 by by IN cord-014901-d9szap94 274 41 an an DT cord-014901-d9szap94 274 42 edible edible JJ cord-014901-d9szap94 274 43 peptide peptide JJ cord-014901-d9szap94 274 44 vaccine vaccine NN cord-014901-d9szap94 274 45 to to IN cord-014901-d9szap94 274 46 bovine bovine JJ cord-014901-d9szap94 274 47 rotavirus rotavirus NN cord-014901-d9szap94 274 48 produced produce VBN cord-014901-d9szap94 274 49 in in IN cord-014901-d9szap94 274 50 transgenic transgenic JJ cord-014901-d9szap94 274 51 plants plant NNS cord-014901-d9szap94 274 52 Bovine bovine JJ cord-014901-d9szap94 274 53 herpes herpe NNS cord-014901-d9szap94 274 54 virus virus NN cord-014901-d9szap94 275 1 gD gD NNP cord-014901-d9szap94 275 2 protein protein NN cord-014901-d9szap94 275 3 pro pro JJ cord-014901-d9szap94 275 4 duced duce VBN cord-014901-d9szap94 275 5 in in IN cord-014901-d9szap94 275 6 plants plant NNS cord-014901-d9szap94 275 7 using use VBG cord-014901-d9szap94 275 8 a a DT cord-014901-d9szap94 275 9 recombinant recombinant JJ cord-014901-d9szap94 275 10 tobacco tobacco NN cord-014901-d9szap94 275 11 mosaic mosaic NN cord-014901-d9szap94 275 12 virus virus NN cord-014901-d9szap94 276 1 ( ( -LRB- cord-014901-d9szap94 276 2 TMV TMV NNP cord-014901-d9szap94 276 3 ) ) -RRB- cord-014901-d9szap94 276 4 vector vector NN cord-014901-d9szap94 276 5 possesses possess VBZ cord-014901-d9szap94 276 6 authentic authentic JJ cord-014901-d9szap94 276 7 antigenicity antigenicity NN cord-014901-d9szap94 276 8 Induction induction NN cord-014901-d9szap94 276 9 of of IN cord-014901-d9szap94 276 10 a a DT cord-014901-d9szap94 276 11 protective protective JJ cord-014901-d9szap94 276 12 antibody antibody NN cord-014901-d9szap94 276 13 response response NN cord-014901-d9szap94 276 14 to to IN cord-014901-d9szap94 276 15 foot foot NN cord-014901-d9szap94 276 16 and and CC cord-014901-d9szap94 276 17 mouth mouth NN cord-014901-d9szap94 276 18 disease disease NN cord-014901-d9szap94 276 19 virus virus NN cord-014901-d9szap94 276 20 in in IN cord-014901-d9szap94 276 21 mice mouse NNS cord-014901-d9szap94 276 22 fol fol NNP cord-014901-d9szap94 276 23 lowing low VBG cord-014901-d9szap94 276 24 oral oral JJ cord-014901-d9szap94 276 25 or or CC cord-014901-d9szap94 276 26 parenteral parenteral JJ cord-014901-d9szap94 276 27 immunization immunization NN cord-014901-d9szap94 276 28 with with IN cord-014901-d9szap94 276 29 alfalfa alfalfa NN cord-014901-d9szap94 276 30 transgenic transgenic JJ cord-014901-d9szap94 276 31 plants plant NNS cord-014901-d9szap94 276 32 expressing express VBG cord-014901-d9szap94 276 33 the the DT cord-014901-d9szap94 276 34 viral viral JJ cord-014901-d9szap94 276 35 structural structural JJ cord-014901-d9szap94 276 36 protein protein NN cord-014901-d9szap94 276 37 VP1 VP1 NNP cord-014901-d9szap94 276 38 Induction Induction NNP cord-014901-d9szap94 276 39 of of IN cord-014901-d9szap94 276 40 a a DT cord-014901-d9szap94 276 41 protective protective JJ cord-014901-d9szap94 276 42 antibody antibody NN cord-014901-d9szap94 276 43 response response NN cord-014901-d9szap94 276 44 to to IN cord-014901-d9szap94 276 45 FMDV FMDV NNP cord-014901-d9szap94 276 46 in in IN cord-014901-d9szap94 276 47 mice mouse NNS cord-014901-d9szap94 276 48 following follow VBG cord-014901-d9szap94 276 49 oral oral JJ cord-014901-d9szap94 276 50 immunization immunization NN cord-014901-d9szap94 276 51 with with IN cord-014901-d9szap94 276 52 transgenic transgenic JJ cord-014901-d9szap94 276 53 Stylosanthes Stylosanthes NNP cord-014901-d9szap94 276 54 spp spp NNS cord-014901-d9szap94 276 55 . . . cord-014901-d9szap94 277 1 as as IN cord-014901-d9szap94 277 2 a a DT cord-014901-d9szap94 277 3 feedstuff feedstuff NNP cord-014901-d9szap94 277 4 additive additive NN cord-014901-d9szap94 277 5 Expression expression NN cord-014901-d9szap94 277 6 of of IN cord-014901-d9szap94 277 7 hemagglutinin hemagglutinin NN cord-014901-d9szap94 277 8 protein protein NN cord-014901-d9szap94 277 9 of of IN cord-014901-d9szap94 277 10 rinderpest rinderpest NN cord-014901-d9szap94 277 11 virus virus NN cord-014901-d9szap94 277 12 in in IN cord-014901-d9szap94 277 13 transgenic transgenic JJ cord-014901-d9szap94 277 14 tobacco tobacco NN cord-014901-d9szap94 277 15 and and CC cord-014901-d9szap94 277 16 immunogenicity immunogenicity NN cord-014901-d9szap94 277 17 of of IN cord-014901-d9szap94 277 18 plant plant NN cord-014901-d9szap94 277 19 derived derive VBN cord-014901-d9szap94 277 20 protein protein NN cord-014901-d9szap94 277 21 in in IN cord-014901-d9szap94 277 22 a a DT cord-014901-d9szap94 277 23 mouse mouse NN cord-014901-d9szap94 277 24 model model NN cord-014901-d9szap94 277 25 Systemic systemic JJ cord-014901-d9szap94 277 26 and and CC cord-014901-d9szap94 277 27 oral oral JJ cord-014901-d9szap94 277 28 immunogenicity immunogenicity NN cord-014901-d9szap94 277 29 of of IN cord-014901-d9szap94 277 30 hemagglutinin hemagglutinin NN cord-014901-d9szap94 277 31 protein protein NN cord-014901-d9szap94 277 32 of of IN cord-014901-d9szap94 277 33 rinderpest rinderpest NN cord-014901-d9szap94 277 34 virus virus NN cord-014901-d9szap94 277 35 expressed express VBN cord-014901-d9szap94 277 36 by by IN cord-014901-d9szap94 277 37 transgenic transgenic JJ cord-014901-d9szap94 277 38 peanut peanut NN cord-014901-d9szap94 277 39 plants plant NNS cord-014901-d9szap94 277 40 in in IN cord-014901-d9szap94 277 41 a a DT cord-014901-d9szap94 277 42 mouse mouse NN cord-014901-d9szap94 277 43 model model NN cord-014901-d9szap94 277 44 Expression expression NN cord-014901-d9szap94 277 45 of of IN cord-014901-d9szap94 277 46 hemagglutinin hemagglutinin NN cord-014901-d9szap94 277 47 protein protein NN cord-014901-d9szap94 277 48 of of IN cord-014901-d9szap94 277 49 rinderpest rinderpest NN cord-014901-d9szap94 277 50 virus virus NN cord-014901-d9szap94 277 51 in in IN cord-014901-d9szap94 277 52 trans trans NNP cord-014901-d9szap94 277 53 genic genic NNP cord-014901-d9szap94 277 54 pigeon pigeon NNP cord-014901-d9szap94 277 55 pea pea NN cord-014901-d9szap94 277 56 Oral oral JJ cord-014901-d9szap94 277 57 immunogenicity immunogenicity NN cord-014901-d9szap94 277 58 of of IN cord-014901-d9szap94 277 59 the the DT cord-014901-d9szap94 277 60 plant plant NN cord-014901-d9szap94 277 61 derived derive VBN cord-014901-d9szap94 277 62 spike spike NN cord-014901-d9szap94 277 63 protein protein NN cord-014901-d9szap94 277 64 from from IN cord-014901-d9szap94 277 65 swine swine NN cord-014901-d9szap94 277 66 transmissible transmissible JJ cord-014901-d9szap94 277 67 gastroenteritis gastroenteritis NN cord-014901-d9szap94 277 68 coronavirus coronavirus NN cord-014901-d9szap94 277 69 Cloning cloning NN cord-014901-d9szap94 277 70 and and CC cord-014901-d9szap94 277 71 sequence sequence NN cord-014901-d9szap94 277 72 analysis analysis NN cord-014901-d9szap94 277 73 of of IN cord-014901-d9szap94 277 74 the the DT cord-014901-d9szap94 277 75 Korean korean JJ cord-014901-d9szap94 277 76 strain strain NN cord-014901-d9szap94 277 77 of of IN cord-014901-d9szap94 277 78 spike spike NN cord-014901-d9szap94 277 79 gene gene NN cord-014901-d9szap94 277 80 of of IN cord-014901-d9szap94 277 81 porcine porcine JJ cord-014901-d9szap94 277 82 epidemic epidemic NN cord-014901-d9szap94 277 83 diarrhea diarrhea NN cord-014901-d9szap94 277 84 virus virus NN cord-014901-d9szap94 277 85 and and CC cord-014901-d9szap94 277 86 expression expression NN cord-014901-d9szap94 277 87 of of IN cord-014901-d9szap94 277 88 its -PRON- PRP$ cord-014901-d9szap94 277 89 neutraliz neutraliz JJ cord-014901-d9szap94 277 90 ing ing DT cord-014901-d9szap94 277 91 epitope epitope NN cord-014901-d9szap94 277 92 in in IN cord-014901-d9szap94 277 93 plants plant NNS cord-014901-d9szap94 278 1 Successful successful JJ cord-014901-d9szap94 278 2 oral oral JJ cord-014901-d9szap94 278 3 prime prime JJ cord-014901-d9szap94 278 4 immunization immunization NN cord-014901-d9szap94 278 5 with with IN cord-014901-d9szap94 278 6 VP60 VP60 NNP cord-014901-d9szap94 278 7 from from IN cord-014901-d9szap94 278 8 rabbit rabbit NN cord-014901-d9szap94 278 9 haemorrhagic haemorrhagic JJ cord-014901-d9szap94 278 10 disease disease NN cord-014901-d9szap94 278 11 virus virus NN cord-014901-d9szap94 278 12 pro pro JJ cord-014901-d9szap94 278 13 duced duce VBN cord-014901-d9szap94 278 14 in in IN cord-014901-d9szap94 278 15 transgenic transgenic JJ cord-014901-d9szap94 278 16 plants plant NNS cord-014901-d9szap94 278 17 using use VBG cord-014901-d9szap94 278 18 different different JJ cord-014901-d9szap94 278 19 fusion fusion NN cord-014901-d9szap94 278 20 strate strate NNP cord-014901-d9szap94 279 1 gies gies NNP cord-014901-d9szap94 280 1 Mucosal Mucosal NNP cord-014901-d9szap94 280 2 and and CC cord-014901-d9szap94 280 3 sys sys NNP cord-014901-d9szap94 280 4 temic temic NNP cord-014901-d9szap94 280 5 immunization immunization NN cord-014901-d9szap94 280 6 elicited elicit VBN cord-014901-d9szap94 280 7 by by IN cord-014901-d9szap94 280 8 Newcastle Newcastle NNP cord-014901-d9szap94 280 9 disease disease NN cord-014901-d9szap94 280 10 virus virus NN cord-014901-d9szap94 280 11 ( ( -LRB- cord-014901-d9szap94 280 12 NDV NDV NNP cord-014901-d9szap94 280 13 ) ) -RRB- cord-014901-d9szap94 280 14 transgenic transgenic JJ cord-014901-d9szap94 280 15 plants plant NNS cord-014901-d9szap94 280 16 as as IN cord-014901-d9szap94 280 17 antigens antigen NNS cord-014901-d9szap94 280 18 Expres Expres NNP cord-014901-d9szap94 280 19 sion sion NN cord-014901-d9szap94 280 20 of of IN cord-014901-d9szap94 280 21 the the DT cord-014901-d9szap94 280 22 fusion fusion NN cord-014901-d9szap94 280 23 glycoprotein glycoprotein NN cord-014901-d9szap94 280 24 of of IN cord-014901-d9szap94 280 25 Newcastle Newcastle NNP cord-014901-d9szap94 280 26 disease disease NN cord-014901-d9szap94 280 27 virus virus NN cord-014901-d9szap94 280 28 in in IN cord-014901-d9szap94 280 29 transgenic transgenic JJ cord-014901-d9szap94 280 30 rice rice NN cord-014901-d9szap94 280 31 and and CC cord-014901-d9szap94 280 32 its -PRON- PRP$ cord-014901-d9szap94 280 33 immunogenicity immunogenicity NN cord-014901-d9szap94 280 34 in in IN cord-014901-d9szap94 280 35 mice mouse NNS cord-014901-d9szap94 280 36 Expression expression NN cord-014901-d9szap94 280 37 of of IN cord-014901-d9szap94 280 38 immunogenic immunogenic JJ cord-014901-d9szap94 280 39 S1 s1 NN cord-014901-d9szap94 280 40 glycoprotein glycoprotein NN cord-014901-d9szap94 280 41 of of IN cord-014901-d9szap94 280 42 infectious infectious JJ cord-014901-d9szap94 280 43 bronchitis bronchitis NN cord-014901-d9szap94 280 44 virus virus NN cord-014901-d9szap94 280 45 in in IN cord-014901-d9szap94 280 46 trans trans NNP cord-014901-d9szap94 280 47 genic genic NNP cord-014901-d9szap94 280 48 potatoes potatoes NNP cord-014901-d9szap94 280 49 Transient transient JJ cord-014901-d9szap94 280 50 expression expression NN cord-014901-d9szap94 280 51 of of IN cord-014901-d9szap94 280 52 the the DT cord-014901-d9szap94 280 53 ectodomain ectodomain NN cord-014901-d9szap94 280 54 of of IN cord-014901-d9szap94 280 55 matrix matrix NN cord-014901-d9szap94 280 56 protein protein NN cord-014901-d9szap94 280 57 2 2 CD cord-014901-d9szap94 280 58 ( ( -LRB- cord-014901-d9szap94 280 59 M2e m2e NN cord-014901-d9szap94 280 60 ) ) -RRB- cord-014901-d9szap94 280 61 of of IN cord-014901-d9szap94 280 62 avian avian JJ cord-014901-d9szap94 280 63 RUSSIAN RUSSIAN NNP cord-014901-d9szap94 280 64 Immunization Immunization NNP cord-014901-d9szap94 280 65 with with IN cord-014901-d9szap94 280 66 plant plant NN cord-014901-d9szap94 280 67 expressed express VBD cord-014901-d9szap94 280 68 hemagglutinin hemagglutinin NN cord-014901-d9szap94 280 69 protects protect VBZ cord-014901-d9szap94 280 70 chickens chicken NNS cord-014901-d9szap94 280 71 from from IN cord-014901-d9szap94 280 72 lethal lethal JJ cord-014901-d9szap94 280 73 highly highly RB cord-014901-d9szap94 280 74 pathogenic pathogenic JJ cord-014901-d9szap94 280 75 avian avian JJ cord-014901-d9szap94 280 76 influenza influenza NN cord-014901-d9szap94 280 77 virus virus NN cord-014901-d9szap94 280 78 H5N1 H5N1 NNP cord-014901-d9szap94 280 79 challenge challenge NN cord-014901-d9szap94 280 80 infection infection NN cord-014901-d9szap94 280 81 Immunogenicity Immunogenicity NNP cord-014901-d9szap94 280 82 study study NN cord-014901-d9szap94 280 83 of of IN cord-014901-d9szap94 280 84 plant plant NN cord-014901-d9szap94 280 85 made make VBD cord-014901-d9szap94 280 86 oral oral JJ cord-014901-d9szap94 280 87 subunit subunit NN cord-014901-d9szap94 280 88 vaccine vaccine NN cord-014901-d9szap94 280 89 against against IN cord-014901-d9szap94 280 90 porcine porcine JJ cord-014901-d9szap94 280 91 reproductive reproductive NN cord-014901-d9szap94 280 92 and and CC cord-014901-d9szap94 280 93 res res FW cord-014901-d9szap94 280 94 piratory piratory NNP cord-014901-d9szap94 280 95 syndrome syndrome NNP cord-014901-d9szap94 280 96 virus virus NN cord-014901-d9szap94 280 97 ( ( -LRB- cord-014901-d9szap94 280 98 PRRSV PRRSV NNP cord-014901-d9szap94 280 99 ) ) -RRB- cord-014901-d9szap94 280 100 Expression expression NN cord-014901-d9szap94 280 101 of of IN cord-014901-d9szap94 280 102 the the DT cord-014901-d9szap94 280 103 rabies rabies NN cord-014901-d9szap94 280 104 virus virus NN cord-014901-d9szap94 280 105 glycoprotein glycoprotein NN cord-014901-d9szap94 280 106 in in IN cord-014901-d9szap94 280 107 transgenic transgenic JJ cord-014901-d9szap94 280 108 tomatoes tomato NNS cord-014901-d9szap94 280 109 Immunization Immunization NNP cord-014901-d9szap94 280 110 against against IN cord-014901-d9szap94 280 111 rabies rabie NNS cord-014901-d9szap94 280 112 with with IN cord-014901-d9szap94 280 113 plant plant NN cord-014901-d9szap94 280 114 derived derive VBN cord-014901-d9szap94 280 115 antigen antigen NN cord-014901-d9szap94 280 116 Development Development NNP cord-014901-d9szap94 280 117 of of IN cord-014901-d9szap94 280 118 an an DT cord-014901-d9szap94 280 119 edible edible JJ cord-014901-d9szap94 280 120 rabies rabie NNS cord-014901-d9szap94 280 121 vaccine vaccine NN cord-014901-d9szap94 280 122 in in IN cord-014901-d9szap94 280 123 maize maize NN cord-014901-d9szap94 280 124 using use VBG cord-014901-d9szap94 280 125 the the DT cord-014901-d9szap94 280 126 Vnukovo Vnukovo NNP cord-014901-d9szap94 280 127 strain strain NN cord-014901-d9szap94 280 128 Induction induction NN cord-014901-d9szap94 280 129 of of IN cord-014901-d9szap94 280 130 a a DT cord-014901-d9szap94 280 131 protective protective JJ cord-014901-d9szap94 280 132 immune immune JJ cord-014901-d9szap94 280 133 response response NN cord-014901-d9szap94 280 134 to to IN cord-014901-d9szap94 280 135 rabies rabie VBN cord-014901-d9szap94 280 136 virus virus NN cord-014901-d9szap94 280 137 in in IN cord-014901-d9szap94 280 138 sheep sheep NN cord-014901-d9szap94 280 139 after after IN cord-014901-d9szap94 280 140 oral oral JJ cord-014901-d9szap94 280 141 immunization immunization NN cord-014901-d9szap94 280 142 with with IN cord-014901-d9szap94 280 143 transgenic transgenic JJ cord-014901-d9szap94 280 144 maize maize NN cord-014901-d9szap94 280 145 , , , cord-014901-d9szap94 280 146 expressing express VBG cord-014901-d9szap94 280 147 the the DT cord-014901-d9szap94 280 148 rabies rabies NN cord-014901-d9szap94 280 149 virus virus NN cord-014901-d9szap94 280 150 glycoprotein glycoprotein NN cord-014901-d9szap94 280 151 Expression expression NN cord-014901-d9szap94 280 152 of of IN cord-014901-d9szap94 280 153 the the DT cord-014901-d9szap94 280 154 rabies rabies NN cord-014901-d9szap94 280 155 virus virus NN cord-014901-d9szap94 280 156 nucleoprotein nucleoprotein NNP cord-014901-d9szap94 280 157 in in IN cord-014901-d9szap94 280 158 plants plant NNS cord-014901-d9szap94 280 159 at at IN cord-014901-d9szap94 280 160 high high JJ cord-014901-d9szap94 280 161 levels level NNS cord-014901-d9szap94 280 162 and and CC cord-014901-d9szap94 280 163 evaluation evaluation NN cord-014901-d9szap94 280 164 of of IN cord-014901-d9szap94 280 165 immune immune JJ cord-014901-d9szap94 280 166 responses response NNS cord-014901-d9szap94 280 167 in in IN cord-014901-d9szap94 280 168 mice mouse NNS cord-014901-d9szap94 280 169 Expression expression NN cord-014901-d9szap94 280 170 of of IN cord-014901-d9szap94 280 171 rabies rabies NN cord-014901-d9szap94 280 172 virus virus NN cord-014901-d9szap94 281 1 G G NNP cord-014901-d9szap94 281 2 pro pro FW cord-014901-d9szap94 281 3 tein tein NN cord-014901-d9szap94 281 4 in in IN cord-014901-d9szap94 281 5 carrots carrot NNS cord-014901-d9szap94 281 6 ( ( -LRB- cord-014901-d9szap94 281 7 Daucus Daucus NNP cord-014901-d9szap94 281 8 carota carota NNP cord-014901-d9szap94 281 9 ) ) -RRB-