id	sid	tid	token	lemma	pos
cord-007211-prygoc0q	1	1	key	key	JJ
cord-007211-prygoc0q	2	1	:	:	:
cord-007211-prygoc0q	2	2	cord-007211-prygoc0q	cord-007211-prygoc0q	JJ
cord-007211-prygoc0q	2	3	authors	author	NNS
cord-007211-prygoc0q	2	4	:	:	:
cord-007211-prygoc0q	2	5	Segawa	Segawa	NNP
cord-007211-prygoc0q	2	6	,	,	,
cord-007211-prygoc0q	2	7	Hiroaki	Hiroaki	NNP
cord-007211-prygoc0q	2	8	;	;	:
cord-007211-prygoc0q	2	9	Kato	Kato	NNP
cord-007211-prygoc0q	2	10	,	,	,
cord-007211-prygoc0q	2	11	Masahiko	Masahiko	NNP
cord-007211-prygoc0q	2	12	;	;	:
cord-007211-prygoc0q	2	13	Yamashita	Yamashita	NNP
cord-007211-prygoc0q	2	14	,	,	,
cord-007211-prygoc0q	2	15	Tetsuro	Tetsuro	NNP
cord-007211-prygoc0q	2	16	;	;	:
cord-007211-prygoc0q	2	17	Taira	Taira	NNP
cord-007211-prygoc0q	2	18	,	,	,
cord-007211-prygoc0q	2	19	Hideharu	Hideharu	NNP
cord-007211-prygoc0q	2	20	title	title	NN
cord-007211-prygoc0q	2	21	:	:	:
cord-007211-prygoc0q	2	22	The	the	DT
cord-007211-prygoc0q	2	23	Roles	Roles	NNPS
cord-007211-prygoc0q	2	24	of	of	IN
cord-007211-prygoc0q	2	25	Individual	Individual	NNP
cord-007211-prygoc0q	2	26	Cysteine	Cysteine	NNP
cord-007211-prygoc0q	2	27	Residues	residue	NNS
cord-007211-prygoc0q	2	28	of	of	IN
cord-007211-prygoc0q	2	29	Sendai	Sendai	NNP
cord-007211-prygoc0q	2	30	Virus	Virus	NNP
cord-007211-prygoc0q	2	31	Fusion	Fusion	NNP
cord-007211-prygoc0q	2	32	Protein	Protein	NNP
cord-007211-prygoc0q	2	33	in	in	IN
cord-007211-prygoc0q	2	34	Intracellular	Intracellular	NNP
cord-007211-prygoc0q	2	35	Transport(1	transport(1	NN
cord-007211-prygoc0q	2	36	)	)	-RRB-
cord-007211-prygoc0q	2	37	date	date	NN
cord-007211-prygoc0q	2	38	:	:	:
cord-007211-prygoc0q	2	39	1998	1998	CD
cord-007211-prygoc0q	2	40	-	-	SYM
cord-007211-prygoc0q	2	41	06	06	CD
cord-007211-prygoc0q	2	42	-	-	SYM
cord-007211-prygoc0q	2	43	17	17	CD
cord-007211-prygoc0q	2	44	journal	journal	NN
cord-007211-prygoc0q	2	45	:	:	:
cord-007211-prygoc0q	2	46	J	J	NNP
cord-007211-prygoc0q	2	47	Biochem	Biochem	NNP
cord-007211-prygoc0q	2	48	DOI	DOI	NNP
cord-007211-prygoc0q	2	49	:	:	:
cord-007211-prygoc0q	2	50	10.1093	10.1093	CD
cord-007211-prygoc0q	2	51	/	/	SYM
cord-007211-prygoc0q	2	52	oxfordjournals.jbchem.a022044	oxfordjournals.jbchem.a022044	CC
cord-007211-prygoc0q	2	53	sha	sha	NNP
cord-007211-prygoc0q	2	54	:	:	:
cord-007211-prygoc0q	2	55	894f168cff3523ff775fd96fe63f5438b98dbc9f	894f168cff3523ff775fd96fe63f5438b98dbc9f	CD
cord-007211-prygoc0q	2	56	doc_id	doc_id	CD
cord-007211-prygoc0q	2	57	:	:	:
cord-007211-prygoc0q	2	58	7211	7211	CD
cord-007211-prygoc0q	2	59	cord_uid	cord_uid	NN
cord-007211-prygoc0q	2	60	:	:	:
cord-007211-prygoc0q	3	1	prygoc0q	prygoc0q	NNP
cord-007211-prygoc0q	4	1	The	the	DT
cord-007211-prygoc0q	4	2	role	role	NN
cord-007211-prygoc0q	4	3	of	of	IN
cord-007211-prygoc0q	4	4	intramolecular	intramolecular	JJ
cord-007211-prygoc0q	4	5	disulfide	disulfide	JJ
cord-007211-prygoc0q	4	6	bonds	bond	NNS
cord-007211-prygoc0q	4	7	in	in	IN
cord-007211-prygoc0q	4	8	the	the	DT
cord-007211-prygoc0q	4	9	fusion	fusion	NN
cord-007211-prygoc0q	4	10	(	(	-LRB-
cord-007211-prygoc0q	4	11	F	F	NNP
cord-007211-prygoc0q	4	12	)	)	-RRB-
cord-007211-prygoc0q	4	13	protein	protein	NN
cord-007211-prygoc0q	4	14	of	of	IN
cord-007211-prygoc0q	4	15	Sendai	Sendai	NNP
cord-007211-prygoc0q	4	16	virus	virus	NN
cord-007211-prygoc0q	4	17	was	be	VBD
cord-007211-prygoc0q	4	18	studied	study	VBN
cord-007211-prygoc0q	4	19	.	.	.
cord-007211-prygoc0q	5	1	The	the	DT
cord-007211-prygoc0q	5	2	10	10	CD
cord-007211-prygoc0q	5	3	cysteine	cysteine	NN
cord-007211-prygoc0q	5	4	residues	residue	NNS
cord-007211-prygoc0q	5	5	were	be	VBD
cord-007211-prygoc0q	5	6	changed	change	VBN
cord-007211-prygoc0q	5	7	to	to	IN
cord-007211-prygoc0q	5	8	serine	serine	NN
cord-007211-prygoc0q	5	9	residues	residue	NNS
cord-007211-prygoc0q	5	10	using	use	VBG
cord-007211-prygoc0q	5	11	site	site	NN
cord-007211-prygoc0q	5	12	-	-	HYPH
cord-007211-prygoc0q	5	13	directed	direct	VBN
cord-007211-prygoc0q	5	14	mutagenesis	mutagenesis	NN
cord-007211-prygoc0q	5	15	.	.	.
cord-007211-prygoc0q	6	1	None	none	NN
cord-007211-prygoc0q	6	2	of	of	IN
cord-007211-prygoc0q	6	3	the	the	DT
cord-007211-prygoc0q	6	4	cysteine	cysteine	NN
cord-007211-prygoc0q	6	5	mutant	mutant	NN
cord-007211-prygoc0q	6	6	F	F	NNP
cord-007211-prygoc0q	6	7	proteins	protein	NNS
cord-007211-prygoc0q	6	8	reacted	react	VBD
cord-007211-prygoc0q	6	9	with	with	IN
cord-007211-prygoc0q	6	10	a	a	DT
cord-007211-prygoc0q	6	11	monoclonal	monoclonal	JJ
cord-007211-prygoc0q	6	12	antibody	antibody	NN
cord-007211-prygoc0q	6	13	specific	specific	JJ
cord-007211-prygoc0q	6	14	for	for	IN
cord-007211-prygoc0q	6	15	the	the	DT
cord-007211-prygoc0q	6	16	mature	mature	JJ
cord-007211-prygoc0q	6	17	conformation	conformation	NN
cord-007211-prygoc0q	6	18	of	of	IN
cord-007211-prygoc0q	6	19	the	the	DT
cord-007211-prygoc0q	6	20	F	F	NNP
cord-007211-prygoc0q	6	21	protein	protein	NN
cord-007211-prygoc0q	6	22	,	,	,
cord-007211-prygoc0q	6	23	but	but	CC
cord-007211-prygoc0q	6	24	eight	eight	CD
cord-007211-prygoc0q	6	25	of	of	IN
cord-007211-prygoc0q	6	26	ten	ten	CD
cord-007211-prygoc0q	6	27	mutants	mutant	NNS
cord-007211-prygoc0q	6	28	reacted	react	VBD
cord-007211-prygoc0q	6	29	with	with	IN
cord-007211-prygoc0q	6	30	an	an	DT
cord-007211-prygoc0q	6	31	immature	immature	JJ
cord-007211-prygoc0q	6	32	conformation	conformation	NN
cord-007211-prygoc0q	6	33	-	-	HYPH
cord-007211-prygoc0q	6	34	specific	specific	JJ
cord-007211-prygoc0q	6	35	monoclonal	monoclonal	JJ
cord-007211-prygoc0q	6	36	antibody	antibody	NN
cord-007211-prygoc0q	6	37	.	.	.
cord-007211-prygoc0q	7	1	The	the	DT
cord-007211-prygoc0q	7	2	transport	transport	NN
cord-007211-prygoc0q	7	3	of	of	IN
cord-007211-prygoc0q	7	4	these	these	DT
cord-007211-prygoc0q	7	5	mutant	mutant	JJ
cord-007211-prygoc0q	7	6	proteins	protein	NNS
cord-007211-prygoc0q	7	7	to	to	IN
cord-007211-prygoc0q	7	8	the	the	DT
cord-007211-prygoc0q	7	9	cell	cell	NN
cord-007211-prygoc0q	7	10	surface	surface	NN
cord-007211-prygoc0q	7	11	was	be	VBD
cord-007211-prygoc0q	7	12	drastically	drastically	RB
cord-007211-prygoc0q	7	13	reduced	reduce	VBN
cord-007211-prygoc0q	7	14	.	.	.
cord-007211-prygoc0q	8	1	All	all	DT
cord-007211-prygoc0q	8	2	of	of	IN
cord-007211-prygoc0q	8	3	the	the	DT
cord-007211-prygoc0q	8	4	cysteine	cysteine	NN
cord-007211-prygoc0q	8	5	mutant	mutant	NN
cord-007211-prygoc0q	8	6	F	F	NNP
cord-007211-prygoc0q	8	7	proteins	protein	NNS
cord-007211-prygoc0q	8	8	remained	remain	VBD
cord-007211-prygoc0q	8	9	sensitive	sensitive	JJ
cord-007211-prygoc0q	8	10	to	to	IN
cord-007211-prygoc0q	8	11	endoglycosidase	endoglycosidase	FW
cord-007211-prygoc0q	8	12	H	H	NNP
cord-007211-prygoc0q	8	13	(	(	-LRB-
cord-007211-prygoc0q	8	14	endo	endo	NN
cord-007211-prygoc0q	8	15	H	H	NNP
cord-007211-prygoc0q	8	16	)	)	-RRB-
cord-007211-prygoc0q	8	17	for	for	IN
cord-007211-prygoc0q	8	18	3	3	CD
cord-007211-prygoc0q	8	19	h	h	NN
cord-007211-prygoc0q	8	20	after	after	IN
cord-007211-prygoc0q	8	21	their	-PRON-	PRP$
cord-007211-prygoc0q	8	22	synthesis	synthesis	NN
cord-007211-prygoc0q	8	23	.	.	.
cord-007211-prygoc0q	9	1	Moreover	moreover	RB
cord-007211-prygoc0q	9	2	,	,	,
cord-007211-prygoc0q	9	3	cell	cell	NN
cord-007211-prygoc0q	9	4	surface	surface	NN
cord-007211-prygoc0q	9	5	transport	transport	NN
cord-007211-prygoc0q	9	6	of	of	IN
cord-007211-prygoc0q	9	7	the	the	DT
cord-007211-prygoc0q	9	8	hemagglutinin	hemagglutinin	NN
cord-007211-prygoc0q	9	9	-	-	HYPH
cord-007211-prygoc0q	9	10	neuraminidase	neuraminidase	VBN
cord-007211-prygoc0q	10	1	(	(	-LRB-
cord-007211-prygoc0q	10	2	HN	HN	NNP
cord-007211-prygoc0q	10	3	)	)	-RRB-
cord-007211-prygoc0q	10	4	protein	protein	NN
cord-007211-prygoc0q	10	5	co	co	NNS
cord-007211-prygoc0q	10	6	-	-	VBD
cord-007211-prygoc0q	10	7	expressed	express	VBN
cord-007211-prygoc0q	10	8	with	with	IN
cord-007211-prygoc0q	10	9	each	each	DT
cord-007211-prygoc0q	10	10	of	of	IN
cord-007211-prygoc0q	10	11	these	these	DT
cord-007211-prygoc0q	10	12	cysteine	cysteine	NN
cord-007211-prygoc0q	10	13	mutant	mutant	NN
cord-007211-prygoc0q	10	14	F	F	NNP
cord-007211-prygoc0q	10	15	proteins	protein	NNS
cord-007211-prygoc0q	10	16	was	be	VBD
cord-007211-prygoc0q	10	17	also	also	RB
cord-007211-prygoc0q	10	18	reduced	reduce	VBN
cord-007211-prygoc0q	10	19	.	.	.
cord-007211-prygoc0q	11	1	These	these	DT
cord-007211-prygoc0q	11	2	results	result	NNS
cord-007211-prygoc0q	11	3	suggest	suggest	VBP
cord-007211-prygoc0q	11	4	that	that	IN
cord-007211-prygoc0q	11	5	all	all	DT
cord-007211-prygoc0q	11	6	cysteine	cysteine	NN
cord-007211-prygoc0q	11	7	residues	residue	NNS
cord-007211-prygoc0q	11	8	participate	participate	VBP
cord-007211-prygoc0q	11	9	in	in	IN
cord-007211-prygoc0q	11	10	the	the	DT
cord-007211-prygoc0q	11	11	formation	formation	NN
cord-007211-prygoc0q	11	12	of	of	IN
cord-007211-prygoc0q	11	13	intramolecular	intramolecular	JJ
cord-007211-prygoc0q	11	14	disulfide	disulfide	JJ
cord-007211-prygoc0q	11	15	bonds	bond	NNS
cord-007211-prygoc0q	11	16	,	,	,
cord-007211-prygoc0q	11	17	that	that	WDT
cord-007211-prygoc0q	11	18	co	co	JJ
cord-007211-prygoc0q	11	19	-	-	JJ
cord-007211-prygoc0q	11	20	translational	translational	JJ
cord-007211-prygoc0q	11	21	disulfide	disulfide	NN
cord-007211-prygoc0q	11	22	bond	bond	NN
cord-007211-prygoc0q	11	23	formation	formation	NN
cord-007211-prygoc0q	11	24	is	be	VBZ
cord-007211-prygoc0q	11	25	crucial	crucial	JJ
cord-007211-prygoc0q	11	26	to	to	IN
cord-007211-prygoc0q	11	27	the	the	DT
cord-007211-prygoc0q	11	28	correct	correct	JJ
cord-007211-prygoc0q	11	29	folding	folding	NN
cord-007211-prygoc0q	11	30	and	and	CC
cord-007211-prygoc0q	11	31	intracellular	intracellular	JJ
cord-007211-prygoc0q	11	32	transport	transport	NN
cord-007211-prygoc0q	11	33	of	of	IN
cord-007211-prygoc0q	11	34	the	the	DT
cord-007211-prygoc0q	11	35	F	F	NNP
cord-007211-prygoc0q	11	36	protein	protein	NN
cord-007211-prygoc0q	11	37	,	,	,
cord-007211-prygoc0q	11	38	and	and	CC
cord-007211-prygoc0q	11	39	that	that	IN
cord-007211-prygoc0q	11	40	interaction	interaction	NN
cord-007211-prygoc0q	11	41	of	of	IN
cord-007211-prygoc0q	11	42	the	the	DT
cord-007211-prygoc0q	11	43	F	F	NNP
cord-007211-prygoc0q	11	44	and	and	CC
cord-007211-prygoc0q	11	45	HN	HN	NNP
cord-007211-prygoc0q	11	46	proteins	protein	NNS
cord-007211-prygoc0q	11	47	takes	take	VBZ
cord-007211-prygoc0q	11	48	place	place	NN
cord-007211-prygoc0q	11	49	intracellulary	intracellulary	NNP
cord-007211-prygoc0q	11	50	.	.	.
cord-007211-prygoc0q	12	1	The	the	DT
cord-007211-prygoc0q	12	2	two	two	CD
cord-007211-prygoc0q	12	3	glycoproteins	glycoprotein	NNS
cord-007211-prygoc0q	12	4	of	of	IN
cord-007211-prygoc0q	12	5	paramyxoviruses	paramyxovirus	NNS
cord-007211-prygoc0q	12	6	,	,	,
cord-007211-prygoc0q	12	7	the	the	DT
cord-007211-prygoc0q	12	8	fusion	fusion	NN
cord-007211-prygoc0q	12	9	(	(	-LRB-
cord-007211-prygoc0q	12	10	F	F	NNP
cord-007211-prygoc0q	12	11	)	)	-RRB-
cord-007211-prygoc0q	12	12	and	and	CC
cord-007211-prygoc0q	12	13	hemagglutinin	hemagglutinin	NN
cord-007211-prygoc0q	12	14	-	-	,
cord-007211-prygoc0q	12	15	neuraminidase	neuraminidase	FW
cord-007211-prygoc0q	12	16	(	(	-LRB-
cord-007211-prygoc0q	12	17	HN	HN	NNP
cord-007211-prygoc0q	12	18	)	)	-RRB-
cord-007211-prygoc0q	12	19	proteins	protein	NNS
cord-007211-prygoc0q	12	20	,	,	,
cord-007211-prygoc0q	12	21	are	be	VBP
cord-007211-prygoc0q	12	22	present	present	JJ
cord-007211-prygoc0q	12	23	as	as	IN
cord-007211-prygoc0q	12	24	integral	integral	JJ
cord-007211-prygoc0q	12	25	proteins	protein	NNS
cord-007211-prygoc0q	12	26	which	which	WDT
cord-007211-prygoc0q	12	27	form	form	VBP
cord-007211-prygoc0q	12	28	spike	spike	NN
cord-007211-prygoc0q	12	29	-	-	HYPH
cord-007211-prygoc0q	12	30	like	like	JJ
cord-007211-prygoc0q	12	31	projections	projection	NNS
cord-007211-prygoc0q	12	32	on	on	IN
cord-007211-prygoc0q	12	33	the	the	DT
cord-007211-prygoc0q	12	34	outer	outer	JJ
cord-007211-prygoc0q	12	35	surface	surface	NN
cord-007211-prygoc0q	12	36	of	of	IN
cord-007211-prygoc0q	12	37	the	the	DT
cord-007211-prygoc0q	12	38	viral	viral	JJ
cord-007211-prygoc0q	12	39	envelope	envelope	NN
cord-007211-prygoc0q	12	40	.	.	.
cord-007211-prygoc0q	13	1	The	the	DT
cord-007211-prygoc0q	13	2	HN	HN	NNP
cord-007211-prygoc0q	13	3	protein	protein	NN
cord-007211-prygoc0q	13	4	exhibits	exhibit	VBZ
cord-007211-prygoc0q	13	5	both	both	CC
cord-007211-prygoc0q	13	6	the	the	DT
cord-007211-prygoc0q	13	7	hemagglutinating	hemagglutinating	NN
cord-007211-prygoc0q	13	8	and	and	CC
cord-007211-prygoc0q	13	9	the	the	DT
cord-007211-prygoc0q	13	10	neuraminidase	neuraminidase	NN
cord-007211-prygoc0q	13	11	activities	activity	NNS
cord-007211-prygoc0q	13	12	,	,	,
cord-007211-prygoc0q	13	13	while	while	IN
cord-007211-prygoc0q	13	14	the	the	DT
cord-007211-prygoc0q	13	15	F	F	NNP
cord-007211-prygoc0q	13	16	protein	protein	NN
cord-007211-prygoc0q	13	17	has	have	VBZ
cord-007211-prygoc0q	13	18	been	be	VBN
cord-007211-prygoc0q	13	19	shown	show	VBN
cord-007211-prygoc0q	13	20	to	to	TO
cord-007211-prygoc0q	13	21	be	be	VB
cord-007211-prygoc0q	13	22	involved	involve	VBN
cord-007211-prygoc0q	13	23	in	in	IN
cord-007211-prygoc0q	13	24	virus	virus	NN
cord-007211-prygoc0q	13	25	penetration	penetration	NN
cord-007211-prygoc0q	13	26	,	,	,
cord-007211-prygoc0q	13	27	hemolysis	hemolysis	NN
cord-007211-prygoc0q	13	28	and	and	CC
cord-007211-prygoc0q	13	29	cell	cell	NN
cord-007211-prygoc0q	13	30	fusion	fusion	NN
cord-007211-prygoc0q	13	31	(	(	-LRB-
cord-007211-prygoc0q	13	32	for	for	IN
cord-007211-prygoc0q	13	33	reviews	review	NNS
cord-007211-prygoc0q	13	34	,	,	,
cord-007211-prygoc0q	13	35	see	see	VB
cord-007211-prygoc0q	13	36	Refs	ref	NNS
cord-007211-prygoc0q	13	37	.	.	.
cord-007211-prygoc0q	14	1	1	1	CD
cord-007211-prygoc0q	14	2	and	and	CC
cord-007211-prygoc0q	14	3	2	2	CD
cord-007211-prygoc0q	14	4	)	)	-RRB-
cord-007211-prygoc0q	14	5	.	.	.
cord-007211-prygoc0q	15	1	The	the	DT
cord-007211-prygoc0q	15	2	F	F	NNP
cord-007211-prygoc0q	15	3	protein	protein	NN
cord-007211-prygoc0q	15	4	is	be	VBZ
cord-007211-prygoc0q	15	5	synthesized	synthesize	VBN
cord-007211-prygoc0q	15	6	as	as	IN
cord-007211-prygoc0q	15	7	an	an	DT
cord-007211-prygoc0q	15	8	inactive	inactive	JJ
cord-007211-prygoc0q	15	9	precursor	precursor	NN
cord-007211-prygoc0q	15	10	,	,	,
cord-007211-prygoc0q	15	11	F	F	NNP
cord-007211-prygoc0q	15	12	o	o	NNP
cord-007211-prygoc0q	15	13	,	,	,
cord-007211-prygoc0q	15	14	which	which	WDT
cord-007211-prygoc0q	15	15	is	be	VBZ
cord-007211-prygoc0q	15	16	cleaved	cleave	VBN
cord-007211-prygoc0q	15	17	by	by	IN
cord-007211-prygoc0q	15	18	proteases	protease	NNS
cord-007211-prygoc0q	15	19	to	to	TO
cord-007211-prygoc0q	15	20	form	form	VB
cord-007211-prygoc0q	15	21	the	the	DT
cord-007211-prygoc0q	15	22	biologically	biologically	RB
cord-007211-prygoc0q	15	23	active	active	JJ
cord-007211-prygoc0q	15	24	protein	protein	NN
cord-007211-prygoc0q	15	25	consisting	consist	VBG
cord-007211-prygoc0q	15	26	of	of	IN
cord-007211-prygoc0q	15	27	the	the	DT
cord-007211-prygoc0q	15	28	disulfide	disulfide	RB
cord-007211-prygoc0q	15	29	-	-	HYPH
cord-007211-prygoc0q	15	30	linked	link	VBN
cord-007211-prygoc0q	15	31	subunits	subunit	NNS
cord-007211-prygoc0q	15	32	Fi	Fi	NNP
cord-007211-prygoc0q	15	33	and	and	CC
cord-007211-prygoc0q	15	34	F	F	NNP
cord-007211-prygoc0q	15	35	2	2	CD
cord-007211-prygoc0q	15	36	.	.	.
cord-007211-prygoc0q	16	1	The	the	DT
cord-007211-prygoc0q	16	2	N	n	CD
cord-007211-prygoc0q	16	3	-	-	HYPH
cord-007211-prygoc0q	16	4	terminal	terminal	JJ
cord-007211-prygoc0q	16	5	portion	portion	NN
cord-007211-prygoc0q	16	6	of	of	IN
cord-007211-prygoc0q	16	7	the	the	DT
cord-007211-prygoc0q	16	8	Fi	Fi	NNP
cord-007211-prygoc0q	16	9	subunit	subunit	NN
cord-007211-prygoc0q	16	10	is	be	VBZ
cord-007211-prygoc0q	16	11	very	very	RB
cord-007211-prygoc0q	16	12	hydrophobic	hydrophobic	JJ
cord-007211-prygoc0q	16	13	,	,	,
cord-007211-prygoc0q	16	14	a	a	DT
cord-007211-prygoc0q	16	15	feature	feature	NN
cord-007211-prygoc0q	16	16	which	which	WDT
cord-007211-prygoc0q	16	17	is	be	VBZ
cord-007211-prygoc0q	16	18	highly	highly	RB
cord-007211-prygoc0q	16	19	conserved	conserve	VBN
cord-007211-prygoc0q	16	20	among	among	IN
cord-007211-prygoc0q	16	21	paramyxovirus	paramyxovirus	NNP
cord-007211-prygoc0q	16	22	F	F	NNP
cord-007211-prygoc0q	16	23	proteins	protein	NNS
cord-007211-prygoc0q	16	24	,	,	,
cord-007211-prygoc0q	16	25	and	and	CC
cord-007211-prygoc0q	16	26	is	be	VBZ
cord-007211-prygoc0q	16	27	suggested	suggest	VBN
cord-007211-prygoc0q	16	28	to	to	TO
cord-007211-prygoc0q	16	29	mediate	mediate	VB
cord-007211-prygoc0q	16	30	fusion	fusion	NN
cord-007211-prygoc0q	16	31	of	of	IN
cord-007211-prygoc0q	16	32	the	the	DT
cord-007211-prygoc0q	16	33	virus	virus	NN
cord-007211-prygoc0q	16	34	envelope	envelope	NN
cord-007211-prygoc0q	16	35	with	with	IN
cord-007211-prygoc0q	16	36	the	the	DT
cord-007211-prygoc0q	16	37	target	target	NN
cord-007211-prygoc0q	16	38	membrane	membrane	NN
cord-007211-prygoc0q	16	39	as	as	RB
cord-007211-prygoc0q	16	40	well	well	RB
cord-007211-prygoc0q	16	41	as	as	IN
cord-007211-prygoc0q	16	42	cell	cell	NN
cord-007211-prygoc0q	16	43	fusion	fusion	NN
cord-007211-prygoc0q	16	44	resulting	result	VBG
cord-007211-prygoc0q	16	45	in	in	IN
cord-007211-prygoc0q	16	46	the	the	DT
cord-007211-prygoc0q	16	47	syncytium	syncytium	NN
cord-007211-prygoc0q	16	48	formation	formation	NN
cord-007211-prygoc0q	16	49	.	.	.
cord-007211-prygoc0q	17	1	The	the	DT
cord-007211-prygoc0q	17	2	sequences	sequence	NNS
cord-007211-prygoc0q	17	3	of	of	IN
cord-007211-prygoc0q	17	4	paramyxovirus	paramyxovirus	NNP
cord-007211-prygoc0q	17	5	F	F	NNP
cord-007211-prygoc0q	17	6	proteins	protein	NNS
cord-007211-prygoc0q	17	7	reveal	reveal	VBP
cord-007211-prygoc0q	17	8	the	the	DT
cord-007211-prygoc0q	17	9	conservation	conservation	NN
cord-007211-prygoc0q	17	10	not	not	RB
cord-007211-prygoc0q	17	11	only	only	RB
cord-007211-prygoc0q	17	12	of	of	IN
cord-007211-prygoc0q	17	13	amino	amino	JJ
cord-007211-prygoc0q	17	14	acid	acid	NN
cord-007211-prygoc0q	17	15	residues	residue	NNS
cord-007211-prygoc0q	17	16	of	of	IN
cord-007211-prygoc0q	17	17	the	the	DT
cord-007211-prygoc0q	17	18	fusioninducing	fusioninduce	VBG
cord-007211-prygoc0q	17	19	domain	domain	NN
cord-007211-prygoc0q	17	20	,	,	,
cord-007211-prygoc0q	17	21	cleavage	cleavage	NN
cord-007211-prygoc0q	17	22	site	site	NN
cord-007211-prygoc0q	17	23	,	,	,
cord-007211-prygoc0q	17	24	and	and	CC
cord-007211-prygoc0q	17	25	transmembrane	transmembrane	NN
cord-007211-prygoc0q	17	26	domain	domain	NN
cord-007211-prygoc0q	17	27	,	,	,
cord-007211-prygoc0q	17	28	but	but	CC
cord-007211-prygoc0q	17	29	also	also	RB
cord-007211-prygoc0q	17	30	of	of	IN
cord-007211-prygoc0q	17	31	cysteine	cysteine	JJ
cord-007211-prygoc0q	17	32	residues	residue	NNS
cord-007211-prygoc0q	17	33	at	at	IN
cord-007211-prygoc0q	17	34	specific	specific	JJ
cord-007211-prygoc0q	17	35	positions	position	NNS
cord-007211-prygoc0q	17	36	.	.	.
cord-007211-prygoc0q	18	1	As	as	IN
cord-007211-prygoc0q	18	2	shown	show	VBN
cord-007211-prygoc0q	18	3	in	in	IN
cord-007211-prygoc0q	18	4	Fig	Fig	NNP
cord-007211-prygoc0q	18	5	.	.	.
cord-007211-prygoc0q	19	1	1	1	LS
cord-007211-prygoc0q	19	2	,	,	,
cord-007211-prygoc0q	19	3	mature	mature	VB
cord-007211-prygoc0q	19	4	Sendai	Sendai	NNP
cord-007211-prygoc0q	19	5	virus	virus	NN
cord-007211-prygoc0q	19	6	F	f	NN
cord-007211-prygoc0q	19	7	o	o	NN
cord-007211-prygoc0q	19	8	protein	protein	NN
cord-007211-prygoc0q	19	9	has	have	VBZ
cord-007211-prygoc0q	19	10	10	10	CD
cord-007211-prygoc0q	19	11	cysteine	cysteine	NN
cord-007211-prygoc0q	19	12	residues	residue	NNS
cord-007211-prygoc0q	19	13	,	,	,
cord-007211-prygoc0q	19	14	designated	designate	VBN
cord-007211-prygoc0q	19	15	Cl	Cl	NNP
cord-007211-prygoc0q	19	16	to	to	IN
cord-007211-prygoc0q	19	17	C10	C10	NNP
cord-007211-prygoc0q	19	18	,	,	,
cord-007211-prygoc0q	19	19	and	and	CC
cord-007211-prygoc0q	19	20	the	the	DT
cord-007211-prygoc0q	19	21	relative	relative	JJ
cord-007211-prygoc0q	19	22	position	position	NN
cord-007211-prygoc0q	19	23	of	of	IN
cord-007211-prygoc0q	19	24	cysteine	cysteine	NN
cord-007211-prygoc0q	19	25	residues	residue	NNS
cord-007211-prygoc0q	19	26	is	be	VBZ
cord-007211-prygoc0q	19	27	highly	highly	RB
cord-007211-prygoc0q	19	28	conserved	conserve	VBN
cord-007211-prygoc0q	19	29	among	among	IN
cord-007211-prygoc0q	19	30	paramyxovirus	paramyxovirus	NNP
cord-007211-prygoc0q	19	31	F	F	NNP
cord-007211-prygoc0q	19	32	proteins	protein	NNS
cord-007211-prygoc0q	19	33	(	(	-LRB-
cord-007211-prygoc0q	19	34	2	2	CD
cord-007211-prygoc0q	19	35	,	,	,
cord-007211-prygoc0q	19	36	3	3	CD
cord-007211-prygoc0q	19	37	)	)	-RRB-
cord-007211-prygoc0q	19	38	.	.	.
cord-007211-prygoc0q	20	1	This	this	DT
cord-007211-prygoc0q	20	2	suggests	suggest	VBZ
cord-007211-prygoc0q	20	3	that	that	IN
cord-007211-prygoc0q	20	4	these	these	DT
cord-007211-prygoc0q	20	5	cysteine	cysteine	NN
cord-007211-prygoc0q	20	6	residues	residue	NNS
cord-007211-prygoc0q	20	7	play	play	VBP
cord-007211-prygoc0q	20	8	important	important	JJ
cord-007211-prygoc0q	20	9	structural	structural	JJ
cord-007211-prygoc0q	20	10	and	and	CC
cord-007211-prygoc0q	20	11	functional	functional	JJ
cord-007211-prygoc0q	20	12	roles	role	NNS
cord-007211-prygoc0q	20	13	.	.	.
cord-007211-prygoc0q	21	1	Two	two	CD
cord-007211-prygoc0q	21	2	cysteine	cysteine	NN
cord-007211-prygoc0q	21	3	residues	residue	NNS
cord-007211-prygoc0q	21	4	are	be	VBP
cord-007211-prygoc0q	21	5	present	present	JJ
cord-007211-prygoc0q	21	6	in	in	IN
cord-007211-prygoc0q	21	7	the	the	DT
cord-007211-prygoc0q	21	8	signal	signal	NN
cord-007211-prygoc0q	21	9	peptide	peptide	NN
cord-007211-prygoc0q	21	10	and	and	CC
cord-007211-prygoc0q	21	11	are	be	VBP
cord-007211-prygoc0q	21	12	not	not	RB
cord-007211-prygoc0q	21	13	likely	likely	JJ
cord-007211-prygoc0q	21	14	to	to	TO
cord-007211-prygoc0q	21	15	participate	participate	VB
cord-007211-prygoc0q	21	16	in	in	IN
cord-007211-prygoc0q	21	17	intramolecular	intramolecular	JJ
cord-007211-prygoc0q	21	18	disulfide	disulfide	JJ
cord-007211-prygoc0q	21	19	bonds	bond	NNS
cord-007211-prygoc0q	21	20	of	of	IN
cord-007211-prygoc0q	21	21	the	the	DT
cord-007211-prygoc0q	21	22	mature	mature	JJ
cord-007211-prygoc0q	21	23	protein	protein	NN
cord-007211-prygoc0q	21	24	.	.	.
cord-007211-prygoc0q	22	1	The	the	DT
cord-007211-prygoc0q	22	2	Cl	Cl	NNP
cord-007211-prygoc0q	22	3	cysteine	cysteine	NN
cord-007211-prygoc0q	22	4	is	be	VBZ
cord-007211-prygoc0q	22	5	in	in	IN
cord-007211-prygoc0q	22	6	the	the	DT
cord-007211-prygoc0q	22	7	F	F	NNP
cord-007211-prygoc0q	22	8	2	2	CD
cord-007211-prygoc0q	22	9	subunit	subunit	NN
cord-007211-prygoc0q	22	10	,	,	,
cord-007211-prygoc0q	22	11	and	and	CC
cord-007211-prygoc0q	22	12	the	the	DT
cord-007211-prygoc0q	22	13	remaining	remain	VBG
cord-007211-prygoc0q	22	14	9	9	CD
cord-007211-prygoc0q	22	15	cysteine	cysteine	NN
cord-007211-prygoc0q	22	16	residues	residue	NNS
cord-007211-prygoc0q	22	17	are	be	VBP
cord-007211-prygoc0q	22	18	in	in	IN
cord-007211-prygoc0q	22	19	the	the	DT
cord-007211-prygoc0q	22	20	Fi	Fi	NNP
cord-007211-prygoc0q	22	21	subunit	subunit	NN
cord-007211-prygoc0q	22	22	.	.	.
cord-007211-prygoc0q	23	1	Eight	eight	CD
cord-007211-prygoc0q	23	2	of	of	IN
cord-007211-prygoc0q	23	3	the	the	DT
cord-007211-prygoc0q	23	4	9	9	CD
cord-007211-prygoc0q	23	5	cysteine	cysteine	NN
cord-007211-prygoc0q	23	6	residues	residue	NNS
cord-007211-prygoc0q	23	7	are	be	VBP
cord-007211-prygoc0q	23	8	clustered	cluster	VBN
cord-007211-prygoc0q	23	9	in	in	IN
cord-007211-prygoc0q	23	10	a	a	DT
cord-007211-prygoc0q	23	11	narrow	narrow	JJ
cord-007211-prygoc0q	23	12	region	region	NN
cord-007211-prygoc0q	23	13	near	near	IN
cord-007211-prygoc0q	23	14	the	the	DT
cord-007211-prygoc0q	23	15	transmembrane	transmembrane	NN
cord-007211-prygoc0q	23	16	domain	domain	NN
cord-007211-prygoc0q	23	17	.	.	.
cord-007211-prygoc0q	24	1	The	the	DT
cord-007211-prygoc0q	24	2	formation	formation	NN
cord-007211-prygoc0q	24	3	of	of	IN
cord-007211-prygoc0q	24	4	disulfide	disulfide	JJ
cord-007211-prygoc0q	24	5	bonds	bond	NNS
cord-007211-prygoc0q	24	6	takes	take	VBZ
cord-007211-prygoc0q	24	7	place	place	NN
cord-007211-prygoc0q	24	8	in	in	IN
cord-007211-prygoc0q	24	9	the	the	DT
cord-007211-prygoc0q	24	10	ER	er	NN
cord-007211-prygoc0q	24	11	and	and	CC
cord-007211-prygoc0q	24	12	is	be	VBZ
cord-007211-prygoc0q	24	13	co	co	NN
cord-007211-prygoc0q	24	14	-	-	NN
cord-007211-prygoc0q	24	15	translationally	translationally	RB
cord-007211-prygoc0q	24	16	catalyzed	catalyze	VBN
cord-007211-prygoc0q	24	17	by	by	IN
cord-007211-prygoc0q	24	18	protein	protein	NN
cord-007211-prygoc0q	24	19	disulfide	disulfide	NN
cord-007211-prygoc0q	24	20	isomerase	isomerase	NN
cord-007211-prygoc0q	24	21	.	.	.
cord-007211-prygoc0q	25	1	It	-PRON-	PRP
cord-007211-prygoc0q	25	2	is	be	VBZ
cord-007211-prygoc0q	25	3	suggested	suggest	VBN
cord-007211-prygoc0q	25	4	that	that	IN
cord-007211-prygoc0q	25	5	only	only	RB
cord-007211-prygoc0q	25	6	native	native	JJ
cord-007211-prygoc0q	25	7	disulfide	disulfide	NN
cord-007211-prygoc0q	25	8	bonds	bond	NNS
cord-007211-prygoc0q	25	9	are	be	VBP
cord-007211-prygoc0q	25	10	found	find	VBN
cord-007211-prygoc0q	25	11	in	in	IN
cord-007211-prygoc0q	25	12	the	the	DT
cord-007211-prygoc0q	25	13	principal	principal	JJ
cord-007211-prygoc0q	25	14	folding	folding	NN
cord-007211-prygoc0q	25	15	intermediates	intermediate	NNS
cord-007211-prygoc0q	25	16	and	and	CC
cord-007211-prygoc0q	25	17	that	that	IN
cord-007211-prygoc0q	25	18	disulfide	disulfide	JJ
cord-007211-prygoc0q	25	19	bond	bond	NN
cord-007211-prygoc0q	25	20	formation	formation	NN
cord-007211-prygoc0q	25	21	plays	play	VBZ
cord-007211-prygoc0q	25	22	an	an	DT
cord-007211-prygoc0q	25	23	integral	integral	JJ
cord-007211-prygoc0q	25	24	role	role	NN
cord-007211-prygoc0q	25	25	in	in	IN
cord-007211-prygoc0q	25	26	the	the	DT
cord-007211-prygoc0q	25	27	folding	folding	NN
cord-007211-prygoc0q	25	28	.	.	.
cord-007211-prygoc0q	26	1	The	the	DT
cord-007211-prygoc0q	26	2	mechanisms	mechanism	NNS
cord-007211-prygoc0q	26	3	of	of	IN
cord-007211-prygoc0q	26	4	folding	folding	NN
cord-007211-prygoc0q	26	5	,	,	,
cord-007211-prygoc0q	26	6	oligomeric	oligomeric	JJ
cord-007211-prygoc0q	26	7	assembly	assembly	NN
cord-007211-prygoc0q	26	8	,	,	,
cord-007211-prygoc0q	26	9	and	and	CC
cord-007211-prygoc0q	26	10	sorting	sorting	NN
cord-007211-prygoc0q	26	11	of	of	IN
cord-007211-prygoc0q	26	12	viral	viral	JJ
cord-007211-prygoc0q	26	13	membrane	membrane	NN
cord-007211-prygoc0q	26	14	proteins	protein	NNS
cord-007211-prygoc0q	26	15	have	have	VBP
cord-007211-prygoc0q	26	16	been	be	VBN
cord-007211-prygoc0q	26	17	characterized	characterize	VBN
cord-007211-prygoc0q	26	18	in	in	IN
cord-007211-prygoc0q	26	19	recent	recent	JJ
cord-007211-prygoc0q	26	20	years	year	NNS
cord-007211-prygoc0q	26	21	(	(	-LRB-
cord-007211-prygoc0q	26	22	for	for	IN
cord-007211-prygoc0q	26	23	review	review	NN
cord-007211-prygoc0q	26	24	,	,	,
cord-007211-prygoc0q	26	25	see	see	VB
cord-007211-prygoc0q	26	26	Ref	Ref	NNP
cord-007211-prygoc0q	26	27	.	.	NNP
cord-007211-prygoc0q	26	28	4	4	LS
cord-007211-prygoc0q	26	29	)	)	-RRB-
cord-007211-prygoc0q	26	30	.	.	.
cord-007211-prygoc0q	27	1	To	to	IN
cord-007211-prygoc0q	27	2	date	date	NN
cord-007211-prygoc0q	27	3	,	,	,
cord-007211-prygoc0q	27	4	the	the	DT
cord-007211-prygoc0q	27	5	roles	role	NNS
cord-007211-prygoc0q	27	6	of	of	IN
cord-007211-prygoc0q	27	7	individual	individual	JJ
cord-007211-prygoc0q	27	8	cysteine	cysteine	NN
cord-007211-prygoc0q	27	9	residues	residue	NNS
cord-007211-prygoc0q	27	10	in	in	IN
cord-007211-prygoc0q	27	11	the	the	DT
cord-007211-prygoc0q	27	12	folding	folding	NN
cord-007211-prygoc0q	27	13	of	of	IN
cord-007211-prygoc0q	27	14	Newcastle	Newcastle	NNP
cord-007211-prygoc0q	27	15	disease	disease	NN
cord-007211-prygoc0q	27	16	virus	virus	NN
cord-007211-prygoc0q	27	17	(	(	-LRB-
cord-007211-prygoc0q	27	18	NDV	NDV	NNP
cord-007211-prygoc0q	27	19	)	)	-RRB-
cord-007211-prygoc0q	27	20	(	(	-LRB-
cord-007211-prygoc0q	27	21	5	5	LS
cord-007211-prygoc0q	27	22	)	)	-RRB-
cord-007211-prygoc0q	27	23	and	and	CC
cord-007211-prygoc0q	27	24	measles	measles	NN
cord-007211-prygoc0q	27	25	virus	virus	NN
cord-007211-prygoc0q	27	26	(	(	-LRB-
cord-007211-prygoc0q	27	27	6	6	LS
cord-007211-prygoc0q	27	28	)	)	-RRB-
cord-007211-prygoc0q	27	29	have	have	VBP
cord-007211-prygoc0q	27	30	been	be	VBN
cord-007211-prygoc0q	27	31	reported	report	VBN
cord-007211-prygoc0q	27	32	.	.	.
cord-007211-prygoc0q	28	1	On	on	IN
cord-007211-prygoc0q	28	2	the	the	DT
cord-007211-prygoc0q	28	3	other	other	JJ
cord-007211-prygoc0q	28	4	hand	hand	NN
cord-007211-prygoc0q	28	5	,	,	,
cord-007211-prygoc0q	28	6	the	the	DT
cord-007211-prygoc0q	28	7	roles	role	NNS
cord-007211-prygoc0q	28	8	of	of	IN
cord-007211-prygoc0q	28	9	individual	individual	JJ
cord-007211-prygoc0q	28	10	cysteine	cysteine	NN
cord-007211-prygoc0q	28	11	residues	residue	NNS
cord-007211-prygoc0q	28	12	of	of	IN
cord-007211-prygoc0q	28	13	paramyxovirus	paramyxovirus	NNP
cord-007211-prygoc0q	28	14	F	F	NNP
cord-007211-prygoc0q	28	15	proteins	protein	NNS
cord-007211-prygoc0q	28	16	have	have	VBP
cord-007211-prygoc0q	28	17	not	not	RB
cord-007211-prygoc0q	28	18	been	be	VBN
cord-007211-prygoc0q	28	19	characterized	characterize	VBN
cord-007211-prygoc0q	28	20	and	and	CC
cord-007211-prygoc0q	28	21	remain	remain	VBP
cord-007211-prygoc0q	28	22	largely	largely	RB
cord-007211-prygoc0q	28	23	unknown	unknown	JJ
cord-007211-prygoc0q	28	24	.	.	.
cord-007211-prygoc0q	29	1	Intramolecular	Intramolecular	NNP
cord-007211-prygoc0q	29	2	and	and	CC
cord-007211-prygoc0q	29	3	intermolecular	intermolecular	JJ
cord-007211-prygoc0q	29	4	disulfide	disulfide	JJ
cord-007211-prygoc0q	29	5	bonds	bond	NNS
cord-007211-prygoc0q	29	6	are	be	VBP
cord-007211-prygoc0q	29	7	essential	essential	JJ
cord-007211-prygoc0q	29	8	components	component	NNS
cord-007211-prygoc0q	29	9	of	of	IN
cord-007211-prygoc0q	29	10	the	the	DT
cord-007211-prygoc0q	29	11	structure	structure	NN
cord-007211-prygoc0q	29	12	of	of	IN
cord-007211-prygoc0q	29	13	the	the	DT
cord-007211-prygoc0q	29	14	majority	majority	NN
cord-007211-prygoc0q	29	15	of	of	IN
cord-007211-prygoc0q	29	16	proteins	protein	NNS
cord-007211-prygoc0q	29	17	,	,	,
cord-007211-prygoc0q	29	18	and	and	CC
cord-007211-prygoc0q	29	19	it	-PRON-	PRP
cord-007211-prygoc0q	29	20	is	be	VBZ
cord-007211-prygoc0q	29	21	of	of	IN
cord-007211-prygoc0q	29	22	great	great	JJ
cord-007211-prygoc0q	29	23	interest	interest	NN
cord-007211-prygoc0q	29	24	to	to	TO
cord-007211-prygoc0q	29	25	understand	understand	VB
cord-007211-prygoc0q	29	26	their	-PRON-	PRP$
cord-007211-prygoc0q	29	27	functions	function	NNS
cord-007211-prygoc0q	29	28	.	.	.
cord-007211-prygoc0q	30	1	Roles	role	NNS
cord-007211-prygoc0q	30	2	of	of	IN
cord-007211-prygoc0q	30	3	disulfide	disulfide	JJ
cord-007211-prygoc0q	30	4	bonds	bond	NNS
cord-007211-prygoc0q	30	5	that	that	WDT
cord-007211-prygoc0q	30	6	have	have	VBP
cord-007211-prygoc0q	30	7	been	be	VBN
cord-007211-prygoc0q	30	8	suggested	suggest	VBN
cord-007211-prygoc0q	30	9	to	to	TO
cord-007211-prygoc0q	30	10	include	include	VB
cord-007211-prygoc0q	30	11	(	(	-LRB-
cord-007211-prygoc0q	30	12	i	i	NN
cord-007211-prygoc0q	30	13	)	)	-RRB-
cord-007211-prygoc0q	30	14	aiding	aid	VBG
cord-007211-prygoc0q	30	15	in	in	IN
cord-007211-prygoc0q	30	16	protein	protein	NN
cord-007211-prygoc0q	30	17	folding	folding	NN
cord-007211-prygoc0q	30	18	and	and	CC
cord-007211-prygoc0q	30	19	maturation	maturation	NN
cord-007211-prygoc0q	31	1	and	and	CC
cord-007211-prygoc0q	31	2	(	(	-LRB-
cord-007211-prygoc0q	31	3	ii	ii	NN
cord-007211-prygoc0q	31	4	)	)	-RRB-
cord-007211-prygoc0q	31	5	maintenance	maintenance	NN
cord-007211-prygoc0q	31	6	of	of	IN
cord-007211-prygoc0q	31	7	stability	stability	NN
cord-007211-prygoc0q	31	8	and	and	CC
cord-007211-prygoc0q	31	9	solubility	solubility	NN
cord-007211-prygoc0q	31	10	.	.	.
cord-007211-prygoc0q	32	1	Two	two	CD
cord-007211-prygoc0q	32	2	general	general	JJ
cord-007211-prygoc0q	32	3	approaches	approach	NNS
cord-007211-prygoc0q	32	4	,	,	,
cord-007211-prygoc0q	32	5	in	in	IN
cord-007211-prygoc0q	32	6	vivo	vivo	NN
cord-007211-prygoc0q	32	7	reduction	reduction	NN
cord-007211-prygoc0q	32	8	using	use	VBG
cord-007211-prygoc0q	32	9	reducing	reduce	VBG
cord-007211-prygoc0q	32	10	reagents	reagent	NNS
cord-007211-prygoc0q	32	11	(	(	-LRB-
cord-007211-prygoc0q	32	12	7	7	CD
cord-007211-prygoc0q	32	13	)	)	-RRB-
cord-007211-prygoc0q	32	14	(	(	-LRB-
cord-007211-prygoc0q	32	15	8)	8)	NNP
cord-007211-prygoc0q	32	16	(	(	-LRB-
cord-007211-prygoc0q	32	17	9	9	CD
cord-007211-prygoc0q	32	18	)	)	-RRB-
cord-007211-prygoc0q	32	19	(	(	-LRB-
cord-007211-prygoc0q	32	20	10	10	CD
cord-007211-prygoc0q	32	21	)	)	-RRB-
cord-007211-prygoc0q	32	22	and	and	CC
cord-007211-prygoc0q	32	23	site	site	NN
cord-007211-prygoc0q	32	24	-	-	HYPH
cord-007211-prygoc0q	32	25	directed	direct	VBN
cord-007211-prygoc0q	32	26	mutagenesis	mutagenesis	NN
cord-007211-prygoc0q	32	27	to	to	TO
cord-007211-prygoc0q	32	28	substitute	substitute	VB
cord-007211-prygoc0q	32	29	each	each	DT
cord-007211-prygoc0q	32	30	of	of	IN
cord-007211-prygoc0q	32	31	the	the	DT
cord-007211-prygoc0q	32	32	cysteine	cysteine	NN
cord-007211-prygoc0q	32	33	residues	residue	NNS
cord-007211-prygoc0q	32	34	with	with	IN
cord-007211-prygoc0q	32	35	other	other	JJ
cord-007211-prygoc0q	32	36	amino	amino	NN
cord-007211-prygoc0q	32	37	acids	acid	NNS
cord-007211-prygoc0q	32	38	(	(	-LRB-
cord-007211-prygoc0q	32	39	5	5	CD
cord-007211-prygoc0q	32	40	,	,	,
cord-007211-prygoc0q	32	41	6	6	CD
cord-007211-prygoc0q	32	42	,	,	,
cord-007211-prygoc0q	32	43	(	(	-LRB-
cord-007211-prygoc0q	32	44	11	11	CD
cord-007211-prygoc0q	32	45	)	)	-RRB-
cord-007211-prygoc0q	32	46	(	(	-LRB-
cord-007211-prygoc0q	32	47	12	12	CD
cord-007211-prygoc0q	32	48	)	)	-RRB-
cord-007211-prygoc0q	32	49	(	(	-LRB-
cord-007211-prygoc0q	32	50	13	13	CD
cord-007211-prygoc0q	32	51	)	)	-RRB-
cord-007211-prygoc0q	32	52	(	(	-LRB-
cord-007211-prygoc0q	32	53	14	14	CD
cord-007211-prygoc0q	32	54	)	)	-RRB-
cord-007211-prygoc0q	32	55	,	,	,
cord-007211-prygoc0q	32	56	have	have	VBP
cord-007211-prygoc0q	32	57	been	be	VBN
cord-007211-prygoc0q	32	58	used	use	VBN
cord-007211-prygoc0q	32	59	to	to	TO
cord-007211-prygoc0q	32	60	study	study	VB
cord-007211-prygoc0q	32	61	the	the	DT
cord-007211-prygoc0q	32	62	functional	functional	JJ
cord-007211-prygoc0q	32	63	role	role	NN
cord-007211-prygoc0q	32	64	of	of	IN
cord-007211-prygoc0q	32	65	disulfide	disulfide	JJ
cord-007211-prygoc0q	32	66	bond	bond	NN
cord-007211-prygoc0q	32	67	formation	formation	NN
cord-007211-prygoc0q	32	68	within	within	IN
cord-007211-prygoc0q	32	69	cells	cell	NNS
cord-007211-prygoc0q	32	70	.	.	.
cord-007211-prygoc0q	33	1	Recently	recently	RB
cord-007211-prygoc0q	33	2	,	,	,
cord-007211-prygoc0q	33	3	the	the	DT
cord-007211-prygoc0q	33	4	sites	site	NNS
cord-007211-prygoc0q	33	5	of	of	IN
cord-007211-prygoc0q	33	6	the	the	DT
cord-007211-prygoc0q	33	7	disulfide	disulfide	JJ
cord-007211-prygoc0q	33	8	bonds	bond	NNS
cord-007211-prygoc0q	33	9	of	of	IN
cord-007211-prygoc0q	33	10	Sendai	Sendai	NNP
cord-007211-prygoc0q	33	11	virus	virus	NN
cord-007211-prygoc0q	33	12	F	F	NNP
cord-007211-prygoc0q	33	13	protein	protein	NN
cord-007211-prygoc0q	33	14	were	be	VBD
cord-007211-prygoc0q	33	15	determined	determine	VBN
cord-007211-prygoc0q	33	16	using	use	VBG
cord-007211-prygoc0q	33	17	protein	protein	NN
cord-007211-prygoc0q	33	18	sequencing	sequencing	NN
cord-007211-prygoc0q	33	19	analysis	analysis	NN
cord-007211-prygoc0q	33	20	,	,	,
cord-007211-prygoc0q	33	21	and	and	CC
cord-007211-prygoc0q	33	22	all	all	DT
cord-007211-prygoc0q	33	23	10	10	CD
cord-007211-prygoc0q	33	24	cysteine	cysteine	NN
cord-007211-prygoc0q	33	25	residues	residue	NNS
cord-007211-prygoc0q	33	26	were	be	VBD
cord-007211-prygoc0q	33	27	shown	show	VBN
cord-007211-prygoc0q	33	28	to	to	TO
cord-007211-prygoc0q	33	29	participate	participate	VB
cord-007211-prygoc0q	33	30	in	in	IN
cord-007211-prygoc0q	33	31	disulfide	disulfide	JJ
cord-007211-prygoc0q	33	32	bond	bond	NN
cord-007211-prygoc0q	33	33	formation	formation	NN
cord-007211-prygoc0q	33	34	{	{	-LRB-
cord-007211-prygoc0q	33	35	15	15	CD
cord-007211-prygoc0q	33	36	)	)	-RRB-
cord-007211-prygoc0q	33	37	.	.	.
cord-007211-prygoc0q	34	1	Thus	thus	RB
cord-007211-prygoc0q	34	2	,	,	,
cord-007211-prygoc0q	34	3	mutagenesis	mutagenesis	NN
cord-007211-prygoc0q	34	4	to	to	TO
cord-007211-prygoc0q	34	5	substitute	substitute	VB
cord-007211-prygoc0q	34	6	each	each	DT
cord-007211-prygoc0q	34	7	cysteine	cysteine	NN
cord-007211-prygoc0q	34	8	residue	residue	NN
cord-007211-prygoc0q	34	9	should	should	MD
cord-007211-prygoc0q	34	10	provide	provide	VB
cord-007211-prygoc0q	34	11	additional	additional	JJ
cord-007211-prygoc0q	34	12	TGC	TGC	NNP
cord-007211-prygoc0q	34	13	-	-	HYPH
cord-007211-prygoc0q	34	14	TCC	TCC	NNP
cord-007211-prygoc0q	34	15	 	 	_SP
cord-007211-prygoc0q	34	16	TGT-»TCT	TGT-»TCT	NNP
cord-007211-prygoc0q	34	17	 	 	_SP
cord-007211-prygoc0q	34	18	TGT	TGT	NNP
cord-007211-prygoc0q	34	19	-	-	HYPH
cord-007211-prygoc0q	34	20	UCT	UCT	NNP
cord-007211-prygoc0q	34	21	 	 	_SP
cord-007211-prygoc0q	34	22	TGOTCC	TGOTCC	NNP
cord-007211-prygoc0q	34	23	 	 	_SP
cord-007211-prygoc0q	35	1	TGT-»TCT	TGT-»TCT	NNP
cord-007211-prygoc0q	35	2	 	 	_SP
cord-007211-prygoc0q	35	3	TGT	TGT	NNP
cord-007211-prygoc0q	35	4	-	-	HYPH
cord-007211-prygoc0q	35	5	UCT	UCT	NNP
cord-007211-prygoc0q	35	6	 	 	_SP
cord-007211-prygoc0q	36	1	TGOTCC	TGOTCC	NNP
cord-007211-prygoc0q	36	2	 	 	_SP
cord-007211-prygoc0q	37	1	TGT-»TCT	TGT-»TCT	NNP
cord-007211-prygoc0q	37	2	 	 	_SP
cord-007211-prygoc0q	38	1	TGC-»TCC	TGC-»TCC	NNP
cord-007211-prygoc0q	38	2	 	 	_SP
cord-007211-prygoc0q	39	1	TGT-»TCT	TGT-»TCT	NNP
cord-007211-prygoc0q	39	2	  	  	_SP
cord-007211-prygoc0q	40	1	A	a	DT
cord-007211-prygoc0q	40	2	findings	finding	NNS
cord-007211-prygoc0q	40	3	regarding	regard	VBG
cord-007211-prygoc0q	40	4	the	the	DT
cord-007211-prygoc0q	40	5	functional	functional	JJ
cord-007211-prygoc0q	40	6	role	role	NN
cord-007211-prygoc0q	40	7	of	of	IN
cord-007211-prygoc0q	40	8	each	each	DT
cord-007211-prygoc0q	40	9	disulfide	disulfide	JJ
cord-007211-prygoc0q	40	10	bond	bond	NN
cord-007211-prygoc0q	40	11	in	in	IN
cord-007211-prygoc0q	40	12	the	the	DT
cord-007211-prygoc0q	40	13	folding	folding	NN
cord-007211-prygoc0q	40	14	,	,	,
cord-007211-prygoc0q	40	15	intracellular	intracellular	JJ
cord-007211-prygoc0q	40	16	transport	transport	NN
cord-007211-prygoc0q	40	17	and	and	CC
cord-007211-prygoc0q	40	18	antigenicity	antigenicity	NN
cord-007211-prygoc0q	40	19	.	.	.
cord-007211-prygoc0q	41	1	Previously	previously	RB
cord-007211-prygoc0q	41	2	,	,	,
cord-007211-prygoc0q	41	3	we	-PRON-	PRP
cord-007211-prygoc0q	41	4	described	describe	VBD
cord-007211-prygoc0q	41	5	the	the	DT
cord-007211-prygoc0q	41	6	efficient	efficient	JJ
cord-007211-prygoc0q	41	7	expression	expression	NN
cord-007211-prygoc0q	41	8	of	of	IN
cord-007211-prygoc0q	41	9	Sendai	Sendai	NNP
cord-007211-prygoc0q	41	10	virus	virus	NN
cord-007211-prygoc0q	41	11	F	F	NNP
cord-007211-prygoc0q	41	12	gene	gene	NN
cord-007211-prygoc0q	41	13	cDNA	cDNA	NNP
cord-007211-prygoc0q	41	14	with	with	IN
cord-007211-prygoc0q	41	15	mutations	mutation	NNS
cord-007211-prygoc0q	41	16	at	at	IN
cord-007211-prygoc0q	41	17	its	-PRON-	PRP$
cord-007211-prygoc0q	41	18	cleavage	cleavage	NN
cord-007211-prygoc0q	41	19	site	site	NN
cord-007211-prygoc0q	41	20	and	and	CC
cord-007211-prygoc0q	41	21	HN	HN	NNP
cord-007211-prygoc0q	41	22	gene	gene	NN
cord-007211-prygoc0q	41	23	cDNA	cdna	NN
cord-007211-prygoc0q	41	24	to	to	TO
cord-007211-prygoc0q	41	25	induce	induce	VB
cord-007211-prygoc0q	41	26	cell	cell	NN
cord-007211-prygoc0q	41	27	fusion	fusion	NN
cord-007211-prygoc0q	41	28	(	(	-LRB-
cord-007211-prygoc0q	41	29	16	16	CD
cord-007211-prygoc0q	41	30	)	)	-RRB-
cord-007211-prygoc0q	41	31	.	.	.
cord-007211-prygoc0q	42	1	In	in	IN
cord-007211-prygoc0q	42	2	this	this	DT
cord-007211-prygoc0q	42	3	study	study	NN
cord-007211-prygoc0q	42	4	,	,	,
cord-007211-prygoc0q	42	5	we	-PRON-	PRP
cord-007211-prygoc0q	42	6	employed	employ	VBD
cord-007211-prygoc0q	42	7	site	site	NN
cord-007211-prygoc0q	42	8	-	-	HYPH
cord-007211-prygoc0q	42	9	directed	direct	VBN
cord-007211-prygoc0q	42	10	mutagenesis	mutagenesis	NN
cord-007211-prygoc0q	42	11	to	to	TO
cord-007211-prygoc0q	42	12	prepare	prepare	VB
cord-007211-prygoc0q	42	13	mutant	mutant	JJ
cord-007211-prygoc0q	42	14	F	F	NNP
cord-007211-prygoc0q	42	15	protein	protein	NN
cord-007211-prygoc0q	42	16	genes	gene	NNS
cord-007211-prygoc0q	42	17	at	at	IN
cord-007211-prygoc0q	42	18	each	each	DT
cord-007211-prygoc0q	42	19	of	of	IN
cord-007211-prygoc0q	42	20	the	the	DT
cord-007211-prygoc0q	42	21	10	10	CD
cord-007211-prygoc0q	42	22	cysteines	cysteine	NNS
cord-007211-prygoc0q	42	23	and	and	CC
cord-007211-prygoc0q	42	24	analyzed	analyze	VBD
cord-007211-prygoc0q	42	25	the	the	DT
cord-007211-prygoc0q	42	26	role	role	NN
cord-007211-prygoc0q	42	27	of	of	IN
cord-007211-prygoc0q	42	28	each	each	DT
cord-007211-prygoc0q	42	29	cysteine	cysteine	NN
cord-007211-prygoc0q	42	30	residue	residue	NN
cord-007211-prygoc0q	42	31	in	in	IN
cord-007211-prygoc0q	42	32	F	F	NNP
cord-007211-prygoc0q	42	33	protein	protein	NN
cord-007211-prygoc0q	42	34	intracellular	intracellular	JJ
cord-007211-prygoc0q	42	35	processing	processing	NN
cord-007211-prygoc0q	42	36	,	,	,
cord-007211-prygoc0q	42	37	cell	cell	NN
cord-007211-prygoc0q	42	38	surface	surface	NN
cord-007211-prygoc0q	42	39	expression	expression	NN
cord-007211-prygoc0q	42	40	,	,	,
cord-007211-prygoc0q	42	41	and	and	CC
cord-007211-prygoc0q	42	42	immunoreactivity	immunoreactivity	NN
cord-007211-prygoc0q	42	43	with	with	IN
cord-007211-prygoc0q	42	44	different	different	JJ
cord-007211-prygoc0q	42	45	monoclonal	monoclonal	JJ
cord-007211-prygoc0q	42	46	antibodies	antibody	NNS
cord-007211-prygoc0q	42	47	(	(	-LRB-
cord-007211-prygoc0q	42	48	MAbs	MAbs	NNP
cord-007211-prygoc0q	42	49	)	)	-RRB-
cord-007211-prygoc0q	42	50	after	after	IN
cord-007211-prygoc0q	42	51	transient	transient	JJ
cord-007211-prygoc0q	42	52	expression	expression	NN
cord-007211-prygoc0q	42	53	of	of	IN
cord-007211-prygoc0q	42	54	the	the	DT
cord-007211-prygoc0q	42	55	cysteine	cysteine	NN
cord-007211-prygoc0q	42	56	mutants	mutant	NNS
cord-007211-prygoc0q	42	57	in	in	IN
cord-007211-prygoc0q	42	58	COS	cos	NN
cord-007211-prygoc0q	42	59	cells	cell	NNS
cord-007211-prygoc0q	42	60	.	.	.
cord-007211-prygoc0q	43	1	Plasmids	Plasmids	NNP
cord-007211-prygoc0q	43	2	-	-	HYPH
cord-007211-prygoc0q	43	3	Mutagenesis	Mutagenesis	NNP
cord-007211-prygoc0q	43	4	of	of	IN
cord-007211-prygoc0q	43	5	PCR	PCR	NNP
cord-007211-prygoc0q	43	6	fragments	fragment	NNS
cord-007211-prygoc0q	43	7	by	by	IN
cord-007211-prygoc0q	43	8	use	use	NN
cord-007211-prygoc0q	43	9	of	of	IN
cord-007211-prygoc0q	43	10	mismatched	mismatched	JJ
cord-007211-prygoc0q	43	11	primers	primer	NNS
cord-007211-prygoc0q	43	12	was	be	VBD
cord-007211-prygoc0q	43	13	performed	perform	VBN
cord-007211-prygoc0q	43	14	in	in	IN
cord-007211-prygoc0q	43	15	a	a	DT
cord-007211-prygoc0q	43	16	three	three	CD
cord-007211-prygoc0q	43	17	-	-	HYPH
cord-007211-prygoc0q	43	18	step	step	NN
cord-007211-prygoc0q	43	19	PCR	PCR	NNP
cord-007211-prygoc0q	43	20	(	(	-LRB-
cord-007211-prygoc0q	43	21	17	17	CD
cord-007211-prygoc0q	43	22	)	)	-RRB-
cord-007211-prygoc0q	43	23	.	.	.
cord-007211-prygoc0q	44	1	To	to	TO
cord-007211-prygoc0q	44	2	replace	replace	VB
cord-007211-prygoc0q	44	3	each	each	DT
cord-007211-prygoc0q	44	4	cysteine	cysteine	NN
cord-007211-prygoc0q	44	5	,	,	,
cord-007211-prygoc0q	44	6	except	except	IN
cord-007211-prygoc0q	44	7	for	for	IN
cord-007211-prygoc0q	44	8	C4	C4	NNP
cord-007211-prygoc0q	44	9	,	,	,
cord-007211-prygoc0q	44	10	we	-PRON-	PRP
cord-007211-prygoc0q	44	11	used	use	VBD
cord-007211-prygoc0q	44	12	the	the	DT
cord-007211-prygoc0q	44	13	sets	set	NNS
cord-007211-prygoc0q	44	14	of	of	IN
cord-007211-prygoc0q	44	15	four	four	CD
cord-007211-prygoc0q	44	16	primers	primer	NNS
cord-007211-prygoc0q	44	17	listed	list	VBN
cord-007211-prygoc0q	44	18	in	in	IN
cord-007211-prygoc0q	44	19	Table	Table	NNP
cord-007211-prygoc0q	44	20	I	-PRON-	PRP
cord-007211-prygoc0q	44	21	.	.	.
cord-007211-prygoc0q	45	1	In	in	IN
cord-007211-prygoc0q	45	2	the	the	DT
cord-007211-prygoc0q	45	3	first	first	JJ
cord-007211-prygoc0q	45	4	PCR	PCR	NNP
cord-007211-prygoc0q	45	5	,	,	,
cord-007211-prygoc0q	45	6	two	two	CD
cord-007211-prygoc0q	45	7	separate	separate	JJ
cord-007211-prygoc0q	45	8	PCR	PCR	NNP
cord-007211-prygoc0q	45	9	reactions	reaction	NNS
cord-007211-prygoc0q	45	10	were	be	VBD
cord-007211-prygoc0q	45	11	run	run	VBN
cord-007211-prygoc0q	45	12	using	use	VBG
cord-007211-prygoc0q	45	13	the	the	DT
cord-007211-prygoc0q	45	14	5'-mutagenic	5'-mutagenic	CD
cord-007211-prygoc0q	45	15	and	and	CC
cord-007211-prygoc0q	45	16	3'-outer	3'-outer	CD
cord-007211-prygoc0q	45	17	primers	primer	NNS
cord-007211-prygoc0q	45	18	and	and	CC
cord-007211-prygoc0q	45	19	the	the	DT
cord-007211-prygoc0q	45	20	3'-mutagenic	3'-mutagenic	CD
cord-007211-prygoc0q	45	21	and	and	CC
cord-007211-prygoc0q	45	22	5'-outer	5'-outer	CD
cord-007211-prygoc0q	45	23	primers	primer	NNS
cord-007211-prygoc0q	45	24	to	to	TO
cord-007211-prygoc0q	45	25	amplify	amplify	VB
cord-007211-prygoc0q	45	26	separate	separate	JJ
cord-007211-prygoc0q	45	27	,	,	,
cord-007211-prygoc0q	45	28	overlapping	overlapping	JJ
cord-007211-prygoc0q	45	29	sequences	sequence	NNS
cord-007211-prygoc0q	45	30	of	of	IN
cord-007211-prygoc0q	45	31	the	the	DT
cord-007211-prygoc0q	45	32	template	template	NN
cord-007211-prygoc0q	45	33	plasmid	plasmid	NN
cord-007211-prygoc0q	45	34	pUC	puc	FW
cord-007211-prygoc0q	45	35	-	-	HYPH
cord-007211-prygoc0q	45	36	F	F	NNP
cord-007211-prygoc0q	45	37	(	(	-LRB-
cord-007211-prygoc0q	45	38	18	18	CD
cord-007211-prygoc0q	45	39	)	)	-RRB-
cord-007211-prygoc0q	45	40	.	.	.
cord-007211-prygoc0q	46	1	The	the	DT
cord-007211-prygoc0q	46	2	5'-mutagenic	5'-mutagenic	CD
cord-007211-prygoc0q	46	3	and	and	CC
cord-007211-prygoc0q	46	4	3'-mutagenic	3'-mutagenic	CD
cord-007211-prygoc0q	46	5	primers	primer	NNS
cord-007211-prygoc0q	46	6	have	have	VBP
cord-007211-prygoc0q	46	7	overlapping	overlap	VBG
cord-007211-prygoc0q	46	8	homologous	homologous	JJ
cord-007211-prygoc0q	46	9	regions	region	NNS
cord-007211-prygoc0q	46	10	containing	contain	VBG
cord-007211-prygoc0q	46	11	a	a	DT
cord-007211-prygoc0q	46	12	mutation	mutation	NN
cord-007211-prygoc0q	46	13	of	of	IN
cord-007211-prygoc0q	46	14	interest	interest	NN
cord-007211-prygoc0q	46	15	.	.	.
cord-007211-prygoc0q	47	1	Following	follow	VBG
cord-007211-prygoc0q	47	2	amplification	amplification	NN
cord-007211-prygoc0q	47	3	,	,	,
cord-007211-prygoc0q	47	4	the	the	DT
cord-007211-prygoc0q	47	5	PCR	PCR	NNP
cord-007211-prygoc0q	47	6	products	product	NNS
cord-007211-prygoc0q	47	7	were	be	VBD
cord-007211-prygoc0q	47	8	purified	purify	VBN
cord-007211-prygoc0q	47	9	,	,	,
cord-007211-prygoc0q	47	10	mixed	mixed	JJ
cord-007211-prygoc0q	47	11	,	,	,
cord-007211-prygoc0q	47	12	then	then	RB
cord-007211-prygoc0q	47	13	denatured	denature	VBN
cord-007211-prygoc0q	47	14	and	and	CC
cord-007211-prygoc0q	47	15	re	re	VBN
cord-007211-prygoc0q	47	16	-	-	VBN
cord-007211-prygoc0q	47	17	annealed	anneal	VBN
cord-007211-prygoc0q	47	18	.	.	.
cord-007211-prygoc0q	48	1	An	an	DT
cord-007211-prygoc0q	48	2	overlapping	overlap	VBG
cord-007211-prygoc0q	48	3	duplex	duplex	NN
cord-007211-prygoc0q	48	4	was	be	VBD
cord-007211-prygoc0q	48	5	formed	form	VBN
cord-007211-prygoc0q	48	6	and	and	CC
cord-007211-prygoc0q	48	7	extended	extend	VBN
cord-007211-prygoc0q	48	8	by	by	IN
cord-007211-prygoc0q	48	9	the	the	DT
cord-007211-prygoc0q	48	10	second	second	JJ
cord-007211-prygoc0q	48	11	PCR	PCR	NNP
cord-007211-prygoc0q	48	12	reaction	reaction	NN
cord-007211-prygoc0q	48	13	without	without	IN
cord-007211-prygoc0q	48	14	primer	primer	NN
cord-007211-prygoc0q	48	15	to	to	TO
cord-007211-prygoc0q	48	16	give	give	VB
cord-007211-prygoc0q	48	17	the	the	DT
cord-007211-prygoc0q	48	18	full	full	JJ
cord-007211-prygoc0q	48	19	-	-	HYPH
cord-007211-prygoc0q	48	20	length	length	NN
cord-007211-prygoc0q	48	21	target	target	NN
cord-007211-prygoc0q	48	22	sequence	sequence	NN
cord-007211-prygoc0q	48	23	containing	contain	VBG
cord-007211-prygoc0q	48	24	the	the	DT
cord-007211-prygoc0q	48	25	mutation	mutation	NN
cord-007211-prygoc0q	48	26	.	.	.
cord-007211-prygoc0q	49	1	This	this	DT
cord-007211-prygoc0q	49	2	mutated	mutate	VBN
cord-007211-prygoc0q	49	3	PCR	pcr	NN
cord-007211-prygoc0q	49	4	fragment	fragment	NN
cord-007211-prygoc0q	49	5	was	be	VBD
cord-007211-prygoc0q	49	6	amplified	amplify	VBN
cord-007211-prygoc0q	49	7	by	by	IN
cord-007211-prygoc0q	49	8	the	the	DT
cord-007211-prygoc0q	49	9	third	third	JJ
cord-007211-prygoc0q	49	10	PCR	PCR	NNP
cord-007211-prygoc0q	49	11	using	use	VBG
cord-007211-prygoc0q	49	12	the	the	DT
cord-007211-prygoc0q	49	13	5'-outer	5'-outer	CD
cord-007211-prygoc0q	49	14	primer	primer	NN
cord-007211-prygoc0q	49	15	and	and	CC
cord-007211-prygoc0q	49	16	3'-outer	3'-outer	CD
cord-007211-prygoc0q	49	17	primer	primer	NN
cord-007211-prygoc0q	49	18	used	use	VBN
cord-007211-prygoc0q	49	19	in	in	IN
cord-007211-prygoc0q	49	20	the	the	DT
cord-007211-prygoc0q	49	21	first	first	JJ
cord-007211-prygoc0q	49	22	PCR	PCR	NNP
cord-007211-prygoc0q	49	23	.	.	.
cord-007211-prygoc0q	50	1	Plasmid	Plasmid	NNP
cord-007211-prygoc0q	50	2	pSRD	pSRD	NNP
cord-007211-prygoc0q	50	3	-	-	HYPH
cord-007211-prygoc0q	50	4	FClS	FClS	NNP
cord-007211-prygoc0q	50	5	was	be	VBD
cord-007211-prygoc0q	50	6	constructed	construct	VBN
cord-007211-prygoc0q	50	7	as	as	IN
cord-007211-prygoc0q	50	8	follows	follow	VBZ
cord-007211-prygoc0q	50	9	:	:	:
cord-007211-prygoc0q	50	10	the	the	DT
cord-007211-prygoc0q	50	11	PCR	PCR	NNP
cord-007211-prygoc0q	50	12	product	product	NN
cord-007211-prygoc0q	50	13	containing	contain	VBG
cord-007211-prygoc0q	50	14	a	a	DT
cord-007211-prygoc0q	50	15	mutation	mutation	NN
cord-007211-prygoc0q	50	16	at	at	IN
cord-007211-prygoc0q	50	17	its	-PRON-	PRP$
cord-007211-prygoc0q	50	18	Cl	Cl	NNP
cord-007211-prygoc0q	50	19	site	site	NN
cord-007211-prygoc0q	50	20	was	be	VBD
cord-007211-prygoc0q	50	21	digested	digest	VBN
cord-007211-prygoc0q	50	22	with	with	IN
cord-007211-prygoc0q	50	23	Clal	Clal	NNP
cord-007211-prygoc0q	50	24	and	and	CC
cord-007211-prygoc0q	50	25	ligated	ligate	VBD
cord-007211-prygoc0q	50	26	to	to	IN
cord-007211-prygoc0q	50	27	the	the	DT
cord-007211-prygoc0q	50	28	Fc	Fc	NNP
cord-007211-prygoc0q	50	29	fragment	fragment	NN
cord-007211-prygoc0q	50	30	,	,	,
cord-007211-prygoc0q	50	31	which	which	WDT
cord-007211-prygoc0q	50	32	was	be	VBD
cord-007211-prygoc0q	50	33	excised	excise	VBN
cord-007211-prygoc0q	50	34	from	from	IN
cord-007211-prygoc0q	50	35	pUC	pUC	NNP
cord-007211-prygoc0q	50	36	-	-	HYPH
cord-007211-prygoc0q	50	37	F	f	NN
cord-007211-prygoc0q	50	38	by	by	IN
cord-007211-prygoc0q	50	39	digestion	digestion	NN
cord-007211-prygoc0q	50	40	with	with	IN
cord-007211-prygoc0q	50	41	Clal	Clal	NNP
cord-007211-prygoc0q	50	42	and	and	CC
cord-007211-prygoc0q	50	43	Hindlll	Hindlll	NNP
cord-007211-prygoc0q	50	44	.	.	.
cord-007211-prygoc0q	51	1	The	the	DT
cord-007211-prygoc0q	51	2	ligated	ligated	JJ
cord-007211-prygoc0q	51	3	whole	whole	JJ
cord-007211-prygoc0q	51	4	F	F	NNP
cord-007211-prygoc0q	51	5	gene	gene	NN
cord-007211-prygoc0q	51	6	was	be	VBD
cord-007211-prygoc0q	51	7	purified	purify	VBN
cord-007211-prygoc0q	51	8	by	by	IN
cord-007211-prygoc0q	51	9	gel	gel	NN
cord-007211-prygoc0q	51	10	extraction	extraction	NN
cord-007211-prygoc0q	51	11	,	,	,
cord-007211-prygoc0q	51	12	treated	treat	VBN
cord-007211-prygoc0q	51	13	with	with	IN
cord-007211-prygoc0q	51	14	Klenow	Klenow	NNP
cord-007211-prygoc0q	51	15	fragment	fragment	NN
cord-007211-prygoc0q	51	16	of	of	IN
cord-007211-prygoc0q	51	17	DNA	dna	NN
cord-007211-prygoc0q	51	18	polymerase	polymerase	NN
cord-007211-prygoc0q	51	19	I	i	NN
cord-007211-prygoc0q	51	20	(	(	-LRB-
cord-007211-prygoc0q	51	21	Klenow	Klenow	NNP
cord-007211-prygoc0q	51	22	fragment	fragment	NN
cord-007211-prygoc0q	51	23	)	)	-RRB-
cord-007211-prygoc0q	51	24	to	to	TO
cord-007211-prygoc0q	51	25	create	create	VB
cord-007211-prygoc0q	51	26	blunt	blunt	JJ
cord-007211-prygoc0q	51	27	ends	end	NNS
cord-007211-prygoc0q	51	28	,	,	,
cord-007211-prygoc0q	51	29	then	then	RB
cord-007211-prygoc0q	51	30	treated	treat	VBN
cord-007211-prygoc0q	51	31	with	with	IN
cord-007211-prygoc0q	51	32	T4	T4	NNP
cord-007211-prygoc0q	51	33	polynucleotide	polynucleotide	NNP
cord-007211-prygoc0q	51	34	kinase	kinase	NN
cord-007211-prygoc0q	51	35	and	and	CC
cord-007211-prygoc0q	51	36	ligated	ligate	VBD
cord-007211-prygoc0q	51	37	into	into	IN
cord-007211-prygoc0q	51	38	the	the	DT
cord-007211-prygoc0q	51	39	expression	expression	NN
cord-007211-prygoc0q	51	40	vector	vector	NN
cord-007211-prygoc0q	51	41	pcDL	pcDL	NNP
cord-007211-prygoc0q	51	42	-	-	HYPH
cord-007211-prygoc0q	51	43	SRD	SRD	NNP
cord-007211-prygoc0q	51	44	(	(	-LRB-
cord-007211-prygoc0q	51	45	pSRD	psrd	NN
cord-007211-prygoc0q	51	46	)	)	-RRB-
cord-007211-prygoc0q	51	47	(	(	-LRB-
cord-007211-prygoc0q	51	48	19	19	CD
cord-007211-prygoc0q	51	49	)	)	-RRB-
cord-007211-prygoc0q	51	50	,	,	,
cord-007211-prygoc0q	51	51	which	which	WDT
cord-007211-prygoc0q	51	52	had	have	VBD
cord-007211-prygoc0q	51	53	been	be	VBN
cord-007211-prygoc0q	51	54	Plasmid	Plasmid	NNP
cord-007211-prygoc0q	51	55	pSRD	pSRD	VBN
cord-007211-prygoc0q	51	56	-	-	HYPH
cord-007211-prygoc0q	51	57	FC2S	FC2S	NNP
cord-007211-prygoc0q	51	58	was	be	VBD
cord-007211-prygoc0q	51	59	constructed	construct	VBN
cord-007211-prygoc0q	51	60	as	as	IN
cord-007211-prygoc0q	51	61	follows	follow	VBZ
cord-007211-prygoc0q	51	62	:	:	:
cord-007211-prygoc0q	51	63	the	the	DT
cord-007211-prygoc0q	51	64	PCR	PCR	NNP
cord-007211-prygoc0q	51	65	product	product	NN
cord-007211-prygoc0q	51	66	containing	contain	VBG
cord-007211-prygoc0q	51	67	a	a	DT
cord-007211-prygoc0q	51	68	mutation	mutation	NN
cord-007211-prygoc0q	51	69	at	at	IN
cord-007211-prygoc0q	51	70	its	-PRON-	PRP$
cord-007211-prygoc0q	51	71	C2	C2	NNP
cord-007211-prygoc0q	51	72	site	site	NN
cord-007211-prygoc0q	51	73	was	be	VBD
cord-007211-prygoc0q	51	74	digested	digest	VBN
cord-007211-prygoc0q	51	75	with	with	IN
cord-007211-prygoc0q	51	76	Bglll	Bglll	NNP
cord-007211-prygoc0q	51	77	and	and	CC
cord-007211-prygoc0q	51	78	Pstl	Pstl	NNP
cord-007211-prygoc0q	51	79	and	and	CC
cord-007211-prygoc0q	51	80	used	use	VBD
cord-007211-prygoc0q	51	81	to	to	TO
cord-007211-prygoc0q	51	82	replace	replace	VB
cord-007211-prygoc0q	51	83	the	the	DT
cord-007211-prygoc0q	51	84	corresponding	corresponding	JJ
cord-007211-prygoc0q	51	85	fragment	fragment	NN
cord-007211-prygoc0q	51	86	of	of	IN
cord-007211-prygoc0q	51	87	pUC	pUC	NNP
cord-007211-prygoc0q	51	88	-	-	HYPH
cord-007211-prygoc0q	51	89	F.	F.	NNP
cord-007211-prygoc0q	51	90	Subsequently	subsequently	RB
cord-007211-prygoc0q	51	91	,	,	,
cord-007211-prygoc0q	51	92	the	the	DT
cord-007211-prygoc0q	51	93	FC2S	FC2S	NNP
cord-007211-prygoc0q	51	94	gene	gene	NN
cord-007211-prygoc0q	51	95	was	be	VBD
cord-007211-prygoc0q	51	96	excised	excise	VBN
cord-007211-prygoc0q	51	97	by	by	IN
cord-007211-prygoc0q	51	98	digestion	digestion	NN
cord-007211-prygoc0q	51	99	with	with	IN
cord-007211-prygoc0q	51	100	Hindlll	Hindlll	NNP
cord-007211-prygoc0q	51	101	,	,	,
cord-007211-prygoc0q	51	102	blunt	blunt	JJ
cord-007211-prygoc0q	51	103	-	-	HYPH
cord-007211-prygoc0q	51	104	ended	end	VBN
cord-007211-prygoc0q	51	105	by	by	IN
cord-007211-prygoc0q	51	106	treatment	treatment	NN
cord-007211-prygoc0q	51	107	with	with	IN
cord-007211-prygoc0q	51	108	Klenow	Klenow	NNP
cord-007211-prygoc0q	51	109	fragment	fragment	NN
cord-007211-prygoc0q	51	110	,	,	,
cord-007211-prygoc0q	51	111	and	and	CC
cord-007211-prygoc0q	51	112	inserted	insert	VBN
cord-007211-prygoc0q	51	113	into	into	IN
cord-007211-prygoc0q	51	114	pSRD	psrd	NN
cord-007211-prygoc0q	51	115	,	,	,
cord-007211-prygoc0q	51	116	which	which	WDT
cord-007211-prygoc0q	51	117	had	have	VBD
cord-007211-prygoc0q	51	118	been	be	VBN
cord-007211-prygoc0q	51	119	linearized	linearize	VBN
cord-007211-prygoc0q	51	120	by	by	IN
cord-007211-prygoc0q	51	121	digesting	digest	VBG
cord-007211-prygoc0q	51	122	with	with	IN
cord-007211-prygoc0q	51	123	£	£	$
cord-007211-prygoc0q	51	124	coRI	cori	NN
cord-007211-prygoc0q	51	125	and	and	CC
cord-007211-prygoc0q	51	126	treated	treat	VBN
cord-007211-prygoc0q	51	127	with	with	IN
cord-007211-prygoc0q	51	128	Klenow	Klenow	NNP
cord-007211-prygoc0q	51	129	fragment	fragment	NN
cord-007211-prygoc0q	51	130	.	.	.
cord-007211-prygoc0q	52	1	To	to	TO
cord-007211-prygoc0q	52	2	construct	construct	VB
cord-007211-prygoc0q	52	3	the	the	DT
cord-007211-prygoc0q	52	4	expression	expression	NN
cord-007211-prygoc0q	52	5	plasmids	plasmid	NNS
cord-007211-prygoc0q	52	6	pSRD	pSRD	VBN
cord-007211-prygoc0q	52	7	-	-	HYPH
cord-007211-prygoc0q	52	8	FC3S	fc3	NNS
cord-007211-prygoc0q	52	9	,	,	,
cord-007211-prygoc0q	53	1	pSRD	pSRD	NNP
cord-007211-prygoc0q	53	2	-	-	HYPH
cord-007211-prygoc0q	53	3	FC5S	fc5s	NN
cord-007211-prygoc0q	53	4	,	,	,
cord-007211-prygoc0q	53	5	pSRD	psrd	CD
cord-007211-prygoc0q	53	6	-	-	HYPH
cord-007211-prygoc0q	53	7	FC6S	FC6S	NNP
cord-007211-prygoc0q	53	8	,	,	,
cord-007211-prygoc0q	53	9	pSRD	pSRD	NNP
cord-007211-prygoc0q	53	10	-	-	HYPH
cord-007211-prygoc0q	53	11	FC7S	FC7S	NNP
cord-007211-prygoc0q	53	12	,	,	.
cord-007211-prygoc0q	54	1	pSRD	pSRD	NNP
cord-007211-prygoc0q	54	2	-	-	HYPH
cord-007211-prygoc0q	54	3	FC8S	FC8S	NNP
cord-007211-prygoc0q	54	4	,	,	,
cord-007211-prygoc0q	55	1	pSRD	pSRD	NNP
cord-007211-prygoc0q	55	2	-	-	HYPH
cord-007211-prygoc0q	55	3	FC9S	fc9s	CD
cord-007211-prygoc0q	55	4	,	,	,
cord-007211-prygoc0q	55	5	and	and	CC
cord-007211-prygoc0q	55	6	pSRD	psrd	NN
cord-007211-prygoc0q	55	7	-	-	HYPH
cord-007211-prygoc0q	55	8	FClOS	FClOS	NNP
cord-007211-prygoc0q	55	9	,	,	,
cord-007211-prygoc0q	55	10	we	-PRON-	PRP
cord-007211-prygoc0q	55	11	used	use	VBD
cord-007211-prygoc0q	55	12	the	the	DT
cord-007211-prygoc0q	55	13	following	follow	VBG
cord-007211-prygoc0q	55	14	procedure	procedure	NN
cord-007211-prygoc0q	55	15	:	:	:
cord-007211-prygoc0q	55	16	a	a	DT
cord-007211-prygoc0q	55	17	cDNA	cdna	NN
cord-007211-prygoc0q	55	18	fragment	fragment	NN
cord-007211-prygoc0q	55	19	containing	contain	VBG
cord-007211-prygoc0q	55	20	the	the	DT
cord-007211-prygoc0q	55	21	carboxyl	carboxyl	NN
cord-007211-prygoc0q	55	22	terminal	terminal	JJ
cord-007211-prygoc0q	55	23	portion	portion	NN
cord-007211-prygoc0q	55	24	of	of	IN
cord-007211-prygoc0q	55	25	F	F	NNP
cord-007211-prygoc0q	55	26	protein	protein	NN
cord-007211-prygoc0q	55	27	from	from	IN
cord-007211-prygoc0q	55	28	235	235	CD
cord-007211-prygoc0q	55	29	to	to	TO
cord-007211-prygoc0q	55	30	565	565	CD
cord-007211-prygoc0q	55	31	amino	amino	NN
cord-007211-prygoc0q	55	32	acid	acid	NN
cord-007211-prygoc0q	55	33	residues	residue	NNS
cord-007211-prygoc0q	55	34	was	be	VBD
cord-007211-prygoc0q	55	35	subcloned	subclone	VBN
cord-007211-prygoc0q	55	36	into	into	IN
cord-007211-prygoc0q	55	37	pBluescript	pBluescript	NNP
cord-007211-prygoc0q	55	38	II	II	NNP
cord-007211-prygoc0q	55	39	SK+	SK+	NNP
cord-007211-prygoc0q	55	40	(	(	-LRB-
cord-007211-prygoc0q	55	41	Stratagene	Stratagene	NNP
cord-007211-prygoc0q	55	42	Cloning	Cloning	NNP
cord-007211-prygoc0q	55	43	Systems	Systems	NNP
cord-007211-prygoc0q	55	44	,	,	,
cord-007211-prygoc0q	55	45	La	La	NNP
cord-007211-prygoc0q	55	46	Jolla	Jolla	NNP
cord-007211-prygoc0q	55	47	,	,	,
cord-007211-prygoc0q	55	48	CA	CA	NNP
cord-007211-prygoc0q	55	49	)	)	-RRB-
cord-007211-prygoc0q	55	50	at	at	IN
cord-007211-prygoc0q	55	51	the	the	DT
cord-007211-prygoc0q	55	52	Pstl	Pstl	NNP
cord-007211-prygoc0q	55	53	site	site	NN
cord-007211-prygoc0q	55	54	to	to	TO
cord-007211-prygoc0q	55	55	yield	yield	VB
cord-007211-prygoc0q	55	56	pBS	pBS	NNP
cord-007211-prygoc0q	55	57	-	-	HYPH
cord-007211-prygoc0q	55	58	Fc	Fc	NNP
cord-007211-prygoc0q	55	59	.	.	.
cord-007211-prygoc0q	56	1	Mutagenesis	Mutagenesis	NNP
cord-007211-prygoc0q	56	2	was	be	VBD
cord-007211-prygoc0q	56	3	performed	perform	VBN
cord-007211-prygoc0q	56	4	as	as	IN
cord-007211-prygoc0q	56	5	described	describe	VBN
cord-007211-prygoc0q	56	6	above	above	RB
cord-007211-prygoc0q	56	7	,	,	,
cord-007211-prygoc0q	56	8	and	and	CC
cord-007211-prygoc0q	56	9	the	the	DT
cord-007211-prygoc0q	56	10	resulting	result	VBG
cord-007211-prygoc0q	56	11	PCR	PCR	NNP
cord-007211-prygoc0q	56	12	products	product	NNS
cord-007211-prygoc0q	56	13	containing	contain	VBG
cord-007211-prygoc0q	56	14	a	a	DT
cord-007211-prygoc0q	56	15	mutation	mutation	NN
cord-007211-prygoc0q	56	16	at	at	IN
cord-007211-prygoc0q	56	17	each	each	DT
cord-007211-prygoc0q	56	18	cysteine	cysteine	NN
cord-007211-prygoc0q	56	19	site	site	NN
cord-007211-prygoc0q	56	20	were	be	VBD
cord-007211-prygoc0q	56	21	digested	digest	VBN
cord-007211-prygoc0q	56	22	with	with	IN
cord-007211-prygoc0q	56	23	Xmnl	Xmnl	NNP
cord-007211-prygoc0q	56	24	and	and	CC
cord-007211-prygoc0q	56	25	Ndel	Ndel	NNP
cord-007211-prygoc0q	56	26	,	,	,
cord-007211-prygoc0q	56	27	then	then	RB
cord-007211-prygoc0q	56	28	inserted	insert	VBD
cord-007211-prygoc0q	56	29	into	into	IN
cord-007211-prygoc0q	56	30	pBS	pBS	NNP
cord-007211-prygoc0q	56	31	-	-	HYPH
cord-007211-prygoc0q	56	32	Fc	Fc	NNP
cord-007211-prygoc0q	56	33	,	,	,
cord-007211-prygoc0q	56	34	which	which	WDT
cord-007211-prygoc0q	56	35	had	have	VBD
cord-007211-prygoc0q	56	36	been	be	VBN
cord-007211-prygoc0q	56	37	digested	digest	VBN
cord-007211-prygoc0q	56	38	with	with	IN
cord-007211-prygoc0q	56	39	Smal	Smal	NNP
cord-007211-prygoc0q	56	40	and	and	CC
cord-007211-prygoc0q	56	41	Ndel	Ndel	NNP
cord-007211-prygoc0q	56	42	.	.	.
cord-007211-prygoc0q	57	1	The	the	DT
cord-007211-prygoc0q	57	2	resulting	result	VBG
cord-007211-prygoc0q	57	3	plasmids	plasmid	NNS
cord-007211-prygoc0q	57	4	were	be	VBD
cord-007211-prygoc0q	57	5	digested	digest	VBN
cord-007211-prygoc0q	57	6	with	with	IN
cord-007211-prygoc0q	57	7	Pstl	Pstl	NNP
cord-007211-prygoc0q	57	8	,	,	,
cord-007211-prygoc0q	57	9	and	and	CC
cord-007211-prygoc0q	57	10	the	the	DT
cord-007211-prygoc0q	57	11	mutated	mutate	VBN
cord-007211-prygoc0q	57	12	Fc	Fc	NNP
cord-007211-prygoc0q	57	13	fragment	fragment	NN
cord-007211-prygoc0q	57	14	was	be	VBD
cord-007211-prygoc0q	57	15	ligated	ligate	VBN
cord-007211-prygoc0q	57	16	with	with	IN
cord-007211-prygoc0q	57	17	4.1	4.1	CD
cord-007211-prygoc0q	57	18	kb	kb	NNP
cord-007211-prygoc0q	57	19	Pstldigested	Pstldigested	NNP
cord-007211-prygoc0q	57	20	pSRD	psrd	CD
cord-007211-prygoc0q	57	21	-	-	HYPH
cord-007211-prygoc0q	57	22	F.	f.	NN
cord-007211-prygoc0q	57	23	Plasmid	Plasmid	NNP
cord-007211-prygoc0q	58	1	pSRD	pSRD	NNP
cord-007211-prygoc0q	58	2	-	-	HYPH
cord-007211-prygoc0q	58	3	FC4S	FC4S	NNP
cord-007211-prygoc0q	58	4	was	be	VBD
cord-007211-prygoc0q	58	5	constructed	construct	VBN
cord-007211-prygoc0q	58	6	as	as	IN
cord-007211-prygoc0q	58	7	follows	follow	VBZ
cord-007211-prygoc0q	58	8	:	:	:
cord-007211-prygoc0q	59	1	PCR	PCR	NNP
cord-007211-prygoc0q	59	2	was	be	VBD
cord-007211-prygoc0q	59	3	performed	perform	VBN
cord-007211-prygoc0q	59	4	using	use	VBG
cord-007211-prygoc0q	59	5	N	n	CD
cord-007211-prygoc0q	59	6	primer	primer	NN
cord-007211-prygoc0q	59	7	and	and	CC
cord-007211-prygoc0q	59	8	mutagenic	mutagenic	JJ
cord-007211-prygoc0q	59	9	primer	primer	NN
cord-007211-prygoc0q	59	10	3'-FlCS3	3'-flcs3	CD
cord-007211-prygoc0q	59	11	.	.	.
cord-007211-prygoc0q	60	1	The	the	DT
cord-007211-prygoc0q	60	2	PCR	PCR	NNP
cord-007211-prygoc0q	60	3	product	product	NN
cord-007211-prygoc0q	60	4	was	be	VBD
cord-007211-prygoc0q	60	5	digested	digest	VBN
cord-007211-prygoc0q	60	6	with	with	IN
cord-007211-prygoc0q	60	7	BamKl	BamKl	NNP
cord-007211-prygoc0q	60	8	and	and	CC
cord-007211-prygoc0q	60	9	inserted	insert	VBD
cord-007211-prygoc0q	60	10	into	into	IN
cord-007211-prygoc0q	60	11	pBS	pBS	NNP
cord-007211-prygoc0q	60	12	-	-	HYPH
cord-007211-prygoc0q	60	13	Fc	Fc	NNP
cord-007211-prygoc0q	60	14	,	,	,
cord-007211-prygoc0q	60	15	which	which	WDT
cord-007211-prygoc0q	60	16	had	have	VBD
cord-007211-prygoc0q	60	17	been	be	VBN
cord-007211-prygoc0q	60	18	digested	digest	VBN
cord-007211-prygoc0q	60	19	with	with	IN
cord-007211-prygoc0q	60	20	the	the	DT
cord-007211-prygoc0q	60	21	same	same	JJ
cord-007211-prygoc0q	60	22	restriction	restriction	NN
cord-007211-prygoc0q	60	23	enzyme	enzyme	NN
cord-007211-prygoc0q	60	24	.	.	.
cord-007211-prygoc0q	61	1	The	the	DT
cord-007211-prygoc0q	61	2	resulting	result	VBG
cord-007211-prygoc0q	61	3	plasmid	plasmid	NN
cord-007211-prygoc0q	61	4	was	be	VBD
cord-007211-prygoc0q	61	5	digested	digest	VBN
cord-007211-prygoc0q	61	6	with	with	IN
cord-007211-prygoc0q	61	7	PstI	PstI	NNP
cord-007211-prygoc0q	61	8	,	,	,
cord-007211-prygoc0q	61	9	and	and	CC
cord-007211-prygoc0q	61	10	the	the	DT
cord-007211-prygoc0q	61	11	mutated	mutate	VBN
cord-007211-prygoc0q	61	12	Fc	Fc	NNP
cord-007211-prygoc0q	61	13	fragment	fragment	NN
cord-007211-prygoc0q	61	14	was	be	VBD
cord-007211-prygoc0q	61	15	ligated	ligate	VBN
cord-007211-prygoc0q	61	16	with	with	IN
cord-007211-prygoc0q	61	17	4.1	4.1	CD
cord-007211-prygoc0q	61	18	kb	kb	NNP
cord-007211-prygoc0q	61	19	PstI	PstI	NNP
cord-007211-prygoc0q	61	20	-	-	HYPH
cord-007211-prygoc0q	61	21	digested	digest	VBN
cord-007211-prygoc0q	61	22	pSRD	psrd	NN
cord-007211-prygoc0q	61	23	-	-	HYPH
cord-007211-prygoc0q	61	24	F	f	NN
cord-007211-prygoc0q	61	25	as	as	IN
cord-007211-prygoc0q	61	26	described	describe	VBN
cord-007211-prygoc0q	61	27	above	above	RB
cord-007211-prygoc0q	61	28	.	.	.
cord-007211-prygoc0q	62	1	The	the	DT
cord-007211-prygoc0q	62	2	mutations	mutation	NNS
cord-007211-prygoc0q	62	3	at	at	IN
cord-007211-prygoc0q	62	4	the	the	DT
cord-007211-prygoc0q	62	5	desired	desire	VBN
cord-007211-prygoc0q	62	6	sites	site	NNS
cord-007211-prygoc0q	62	7	of	of	IN
cord-007211-prygoc0q	62	8	all	all	PDT
cord-007211-prygoc0q	62	9	the	the	DT
cord-007211-prygoc0q	62	10	mutant	mutant	JJ
cord-007211-prygoc0q	62	11	DNAs	dna	NNS
cord-007211-prygoc0q	62	12	were	be	VBD
cord-007211-prygoc0q	62	13	confirmed	confirm	VBN
cord-007211-prygoc0q	62	14	by	by	IN
cord-007211-prygoc0q	62	15	DNA	dna	NN
cord-007211-prygoc0q	62	16	sequencing	sequencing	NN
cord-007211-prygoc0q	62	17	(	(	-LRB-
cord-007211-prygoc0q	62	18	20	20	CD
cord-007211-prygoc0q	62	19	)	)	-RRB-
cord-007211-prygoc0q	62	20	.	.	.
cord-007211-prygoc0q	63	1	As	as	IN
cord-007211-prygoc0q	63	2	shown	show	VBN
cord-007211-prygoc0q	63	3	in	in	IN
cord-007211-prygoc0q	63	4	Fig	Fig	NNP
cord-007211-prygoc0q	63	5	.	.	.
cord-007211-prygoc0q	64	1	1	1	CD
cord-007211-prygoc0q	64	2	,	,	,
cord-007211-prygoc0q	64	3	a	a	DT
cord-007211-prygoc0q	64	4	total	total	NN
cord-007211-prygoc0q	64	5	of	of	IN
cord-007211-prygoc0q	64	6	10	10	CD
cord-007211-prygoc0q	64	7	cysteine	cysteine	NN
cord-007211-prygoc0q	64	8	mutants	mutant	NNS
cord-007211-prygoc0q	64	9	were	be	VBD
cord-007211-prygoc0q	64	10	prepared	prepare	VBN
cord-007211-prygoc0q	64	11	and	and	CC
cord-007211-prygoc0q	64	12	designated	designate	VBN
cord-007211-prygoc0q	64	13	FClS	FClS	NNP
cord-007211-prygoc0q	64	14	to	to	IN
cord-007211-prygoc0q	64	15	FC10S	fc10	NNS
cord-007211-prygoc0q	64	16	from	from	IN
cord-007211-prygoc0q	64	17	the	the	DT
cord-007211-prygoc0q	64	18	amino	amino	NN
cord-007211-prygoc0q	64	19	terminus	terminus	NN
cord-007211-prygoc0q	64	20	of	of	IN
cord-007211-prygoc0q	64	21	the	the	DT
cord-007211-prygoc0q	64	22	F	F	NNP
cord-007211-prygoc0q	64	23	o	o	NN
cord-007211-prygoc0q	64	24	protein	protein	NN
cord-007211-prygoc0q	64	25	to	to	IN
cord-007211-prygoc0q	64	26	the	the	DT
cord-007211-prygoc0q	64	27	carboxyl	carboxyl	NN
cord-007211-prygoc0q	64	28	terminus	terminus	NN
cord-007211-prygoc0q	64	29	,	,	,
cord-007211-prygoc0q	64	30	corresponding	correspond	VBG
cord-007211-prygoc0q	64	31	to	to	IN
cord-007211-prygoc0q	64	32	the	the	DT
cord-007211-prygoc0q	64	33	cysteine	cysteine	NN
cord-007211-prygoc0q	64	34	residues	residue	NNS
cord-007211-prygoc0q	64	35	at	at	IN
cord-007211-prygoc0q	64	36	amino	amino	NN
cord-007211-prygoc0q	64	37	acid	acid	NN
cord-007211-prygoc0q	64	38	positions	position	NNS
cord-007211-prygoc0q	64	39	70	70	CD
cord-007211-prygoc0q	64	40	,	,	,
cord-007211-prygoc0q	64	41	199	199	CD
cord-007211-prygoc0q	64	42	,	,	,
cord-007211-prygoc0q	64	43	338	338	CD
cord-007211-prygoc0q	64	44	,	,	,
cord-007211-prygoc0q	64	45	347	347	CD
cord-007211-prygoc0q	64	46	,	,	,
cord-007211-prygoc0q	64	47	362	362	CD
cord-007211-prygoc0q	64	48	,	,	,
cord-007211-prygoc0q	64	49	370	370	CD
cord-007211-prygoc0q	64	50	,	,	,
cord-007211-prygoc0q	64	51	394	394	CD
cord-007211-prygoc0q	64	52	,	,	,
cord-007211-prygoc0q	64	53	399	399	CD
cord-007211-prygoc0q	64	54	,	,	,
cord-007211-prygoc0q	64	55	401	401	CD
cord-007211-prygoc0q	64	56	,	,	,
cord-007211-prygoc0q	64	57	and	and	CC
cord-007211-prygoc0q	64	58	424	424	CD
cord-007211-prygoc0q	64	59	,	,	,
cord-007211-prygoc0q	64	60	respectively	respectively	RB
cord-007211-prygoc0q	64	61	.	.	.
cord-007211-prygoc0q	65	1	The	the	DT
cord-007211-prygoc0q	65	2	expression	expression	NN
cord-007211-prygoc0q	65	3	plasmid	plasmid	NN
cord-007211-prygoc0q	65	4	pSRD	pSRD	NNP
cord-007211-prygoc0q	65	5	-	-	HYPH
cord-007211-prygoc0q	65	6	HN	HN	NNP
cord-007211-prygoc0q	65	7	was	be	VBD
cord-007211-prygoc0q	65	8	constructed	construct	VBN
cord-007211-prygoc0q	65	9	by	by	IN
cord-007211-prygoc0q	65	10	inserting	insert	VBG
cord-007211-prygoc0q	65	11	the	the	DT
cord-007211-prygoc0q	65	12	whole	whole	JJ
cord-007211-prygoc0q	65	13	HN	HN	NNP
cord-007211-prygoc0q	65	14	gene	gene	NN
cord-007211-prygoc0q	65	15	into	into	IN
cord-007211-prygoc0q	65	16	pSRD	psrd	CD
cord-007211-prygoc0q	65	17	as	as	IN
cord-007211-prygoc0q	65	18	described	describe	VBN
cord-007211-prygoc0q	65	19	previously	previously	RB
cord-007211-prygoc0q	65	20	(	(	-LRB-
cord-007211-prygoc0q	65	21	16	16	CD
cord-007211-prygoc0q	65	22	)	)	-RRB-
cord-007211-prygoc0q	65	23	.	.	.
cord-007211-prygoc0q	66	1	Cell	cell	NN
cord-007211-prygoc0q	66	2	Culture	culture	NN
cord-007211-prygoc0q	66	3	and	and	CC
cord-007211-prygoc0q	66	4	DNA	DNA	NNP
cord-007211-prygoc0q	66	5	Transfection	Transfection	NNP
cord-007211-prygoc0q	66	6	-	-	HYPH
cord-007211-prygoc0q	66	7	Monkey	Monkey	NNP
cord-007211-prygoc0q	66	8	COS-1	COS-1	NNP
cord-007211-prygoc0q	66	9	cells	cell	NNS
cord-007211-prygoc0q	66	10	(	(	-LRB-
cord-007211-prygoc0q	66	11	21	21	CD
cord-007211-prygoc0q	66	12	)	)	-RRB-
cord-007211-prygoc0q	66	13	were	be	VBD
cord-007211-prygoc0q	66	14	grown	grow	VBN
cord-007211-prygoc0q	66	15	in	in	IN
cord-007211-prygoc0q	66	16	Dulbecco	Dulbecco	NNP
cord-007211-prygoc0q	66	17	's	's	POS
cord-007211-prygoc0q	66	18	modified	modify	VBN
cord-007211-prygoc0q	66	19	Eagle	Eagle	NNP
cord-007211-prygoc0q	66	20	's	's	POS
cord-007211-prygoc0q	66	21	medium	medium	NN
cord-007211-prygoc0q	66	22	(	(	-LRB-
cord-007211-prygoc0q	66	23	DMEM	DMEM	NNP
cord-007211-prygoc0q	66	24	)	)	-RRB-
cord-007211-prygoc0q	66	25	supplemented	supplement	VBD
cord-007211-prygoc0q	66	26	with	with	IN
cord-007211-prygoc0q	66	27	10	10	CD
cord-007211-prygoc0q	66	28	%	%	NN
cord-007211-prygoc0q	66	29	fetal	fetal	JJ
cord-007211-prygoc0q	66	30	calf	calf	NN
cord-007211-prygoc0q	66	31	serum	serum	NN
cord-007211-prygoc0q	66	32	at	at	IN
cord-007211-prygoc0q	66	33	37	37	CD
cord-007211-prygoc0q	66	34	°	°	NN
cord-007211-prygoc0q	66	35	C	c	NN
cord-007211-prygoc0q	66	36	in	in	IN
cord-007211-prygoc0q	66	37	5	5	CD
cord-007211-prygoc0q	66	38	%	%	NN
cord-007211-prygoc0q	66	39	CO	co	NN
cord-007211-prygoc0q	66	40	2	2	CD
cord-007211-prygoc0q	66	41	.	.	.
cord-007211-prygoc0q	67	1	For	for	IN
cord-007211-prygoc0q	67	2	transient	transient	JJ
cord-007211-prygoc0q	67	3	expression	expression	NN
cord-007211-prygoc0q	67	4	of	of	IN
cord-007211-prygoc0q	67	5	the	the	DT
cord-007211-prygoc0q	67	6	F	F	NNP
cord-007211-prygoc0q	67	7	protein	protein	NN
cord-007211-prygoc0q	67	8	,	,	,
cord-007211-prygoc0q	67	9	COS	cos	NN
cord-007211-prygoc0q	67	10	cells	cell	NNS
cord-007211-prygoc0q	67	11	were	be	VBD
cord-007211-prygoc0q	67	12	grown	grow	VBN
cord-007211-prygoc0q	67	13	to	to	IN
cord-007211-prygoc0q	67	14	80	80	CD
cord-007211-prygoc0q	67	15	%	%	NN
cord-007211-prygoc0q	67	16	confluency	confluency	NN
cord-007211-prygoc0q	67	17	in	in	IN
cord-007211-prygoc0q	67	18	35-mm	35-mm	CD
cord-007211-prygoc0q	67	19	tissue	tissue	NN
cord-007211-prygoc0q	67	20	culture	culture	NN
cord-007211-prygoc0q	67	21	dishes	dish	NNS
cord-007211-prygoc0q	67	22	,	,	,
cord-007211-prygoc0q	67	23	then	then	RB
cord-007211-prygoc0q	67	24	transfected	transfecte	VBN
cord-007211-prygoc0q	67	25	with	with	IN
cord-007211-prygoc0q	67	26	5	5	CD
cord-007211-prygoc0q	67	27	jug	jug	NN
cord-007211-prygoc0q	67	28	of	of	IN
cord-007211-prygoc0q	67	29	expression	expression	NN
cord-007211-prygoc0q	67	30	plasmid	plasmid	NN
cord-007211-prygoc0q	67	31	per	per	IN
cord-007211-prygoc0q	67	32	dish	dish	NN
cord-007211-prygoc0q	67	33	by	by	IN
cord-007211-prygoc0q	67	34	calcium	calcium	NN
cord-007211-prygoc0q	67	35	phosphate	phosphate	NN
cord-007211-prygoc0q	67	36	precipitation	precipitation	NN
cord-007211-prygoc0q	67	37	(	(	-LRB-
cord-007211-prygoc0q	67	38	22	22	CD
cord-007211-prygoc0q	67	39	)	)	-RRB-
cord-007211-prygoc0q	67	40	.	.	.
cord-007211-prygoc0q	68	1	Cells	cell	NNS
cord-007211-prygoc0q	68	2	incubated	incubate	VBN
cord-007211-prygoc0q	68	3	for	for	IN
cord-007211-prygoc0q	68	4	48	48	CD
cord-007211-prygoc0q	68	5	h	h	NN
cord-007211-prygoc0q	68	6	after	after	IN
cord-007211-prygoc0q	68	7	transfection	transfection	NN
cord-007211-prygoc0q	68	8	were	be	VBD
cord-007211-prygoc0q	68	9	analyzed	analyze	VBN
cord-007211-prygoc0q	68	10	by	by	IN
cord-007211-prygoc0q	68	11	immunoprecipitation	immunoprecipitation	NN
cord-007211-prygoc0q	68	12	(	(	-LRB-
cord-007211-prygoc0q	68	13	18	18	CD
cord-007211-prygoc0q	68	14	)	)	-RRB-
cord-007211-prygoc0q	68	15	.	.	.
cord-007211-prygoc0q	69	1	Antibodies	antibody	NNS
cord-007211-prygoc0q	69	2	-	-	HYPH
cord-007211-prygoc0q	69	3	Anti	Anti	NNP
cord-007211-prygoc0q	69	4	-	-	NNP
cord-007211-prygoc0q	69	5	Sendai	Sendai	NNP
cord-007211-prygoc0q	69	6	virus	virus	NN
cord-007211-prygoc0q	69	7	antiserum	antiserum	NN
cord-007211-prygoc0q	69	8	was	be	VBD
cord-007211-prygoc0q	69	9	prepared	prepare	VBN
cord-007211-prygoc0q	69	10	from	from	IN
cord-007211-prygoc0q	69	11	Sendai	Sendai	NNP
cord-007211-prygoc0q	69	12	virus	virus	NN
cord-007211-prygoc0q	69	13	-	-	HYPH
cord-007211-prygoc0q	69	14	infected	infect	VBN
cord-007211-prygoc0q	69	15	rabbits	rabbit	NNS
cord-007211-prygoc0q	69	16	.	.	.
cord-007211-prygoc0q	70	1	The	the	DT
cord-007211-prygoc0q	70	2	antiserum	antiserum	NN
cord-007211-prygoc0q	70	3	used	use	VBD
cord-007211-prygoc0q	70	4	for	for	IN
cord-007211-prygoc0q	70	5	immunoprecipitation	immunoprecipitation	NN
cord-007211-prygoc0q	70	6	was	be	VBD
cord-007211-prygoc0q	70	7	obtained	obtain	VBN
cord-007211-prygoc0q	70	8	from	from	IN
cord-007211-prygoc0q	70	9	rabbits	rabbit	NNS
cord-007211-prygoc0q	70	10	immunized	immunize	VBN
cord-007211-prygoc0q	70	11	with	with	IN
cord-007211-prygoc0q	70	12	F	F	NNP
cord-007211-prygoc0q	70	13	proteins	protein	NNS
cord-007211-prygoc0q	70	14	,	,	,
cord-007211-prygoc0q	70	15	which	which	WDT
cord-007211-prygoc0q	70	16	were	be	VBD
cord-007211-prygoc0q	70	17	expressed	express	VBN
cord-007211-prygoc0q	70	18	in	in	IN
cord-007211-prygoc0q	70	19	Escherichia	Escherichia	NNP
cord-007211-prygoc0q	70	20	coli	coli	NNS
cord-007211-prygoc0q	70	21	as	as	IN
cord-007211-prygoc0q	70	22	maltose	maltose	NN
cord-007211-prygoc0q	70	23	-	-	HYPH
cord-007211-prygoc0q	70	24	binding	bind	VBG
cord-007211-prygoc0q	70	25	fusion	fusion	NN
cord-007211-prygoc0q	70	26	proteins	protein	NNS
cord-007211-prygoc0q	70	27	and	and	CC
cord-007211-prygoc0q	70	28	purified	purify	VBN
cord-007211-prygoc0q	70	29	by	by	IN
cord-007211-prygoc0q	70	30	amylose	amylose	JJ
cord-007211-prygoc0q	70	31	resin	resin	NN
cord-007211-prygoc0q	70	32	as	as	IN
cord-007211-prygoc0q	70	33	described	describe	VBN
cord-007211-prygoc0q	70	34	by	by	IN
cord-007211-prygoc0q	70	35	the	the	DT
cord-007211-prygoc0q	70	36	manufacturer	manufacturer	NN
cord-007211-prygoc0q	70	37	(	(	-LRB-
cord-007211-prygoc0q	70	38	New	New	NNP
cord-007211-prygoc0q	70	39	England	England	NNP
cord-007211-prygoc0q	70	40	Biolabs	Biolabs	NNP
cord-007211-prygoc0q	70	41	,	,	,
cord-007211-prygoc0q	70	42	Beverly	Beverly	NNP
cord-007211-prygoc0q	70	43	,	,	,
cord-007211-prygoc0q	70	44	Massachusetts	Massachusetts	NNP
cord-007211-prygoc0q	70	45	)	)	-RRB-
cord-007211-prygoc0q	70	46	.	.	.
cord-007211-prygoc0q	71	1	Anti	Anti	NNP
cord-007211-prygoc0q	71	2	-	-	NNP
cord-007211-prygoc0q	71	3	F	F	NNP
cord-007211-prygoc0q	71	4	serum	serum	NN
cord-007211-prygoc0q	71	5	used	use	VBN
cord-007211-prygoc0q	71	6	to	to	TO
cord-007211-prygoc0q	71	7	detect	detect	VB
cord-007211-prygoc0q	71	8	cell	cell	NN
cord-007211-prygoc0q	71	9	surface	surface	NN
cord-007211-prygoc0q	71	10	or	or	CC
cord-007211-prygoc0q	71	11	intracellular	intracellular	JJ
cord-007211-prygoc0q	71	12	expression	expression	NN
cord-007211-prygoc0q	71	13	of	of	IN
cord-007211-prygoc0q	71	14	the	the	DT
cord-007211-prygoc0q	71	15	F	F	NNP
cord-007211-prygoc0q	71	16	protein	protein	NN
cord-007211-prygoc0q	71	17	was	be	VBD
cord-007211-prygoc0q	71	18	obtained	obtain	VBN
cord-007211-prygoc0q	71	19	from	from	IN
cord-007211-prygoc0q	71	20	rabbits	rabbit	NNS
cord-007211-prygoc0q	71	21	immunized	immunize	VBN
cord-007211-prygoc0q	71	22	with	with	IN
cord-007211-prygoc0q	71	23	the	the	DT
cord-007211-prygoc0q	71	24	Fj	Fj	NNP
cord-007211-prygoc0q	71	25	protein	protein	NN
cord-007211-prygoc0q	71	26	,	,	,
cord-007211-prygoc0q	71	27	which	which	WDT
cord-007211-prygoc0q	71	28	was	be	VBD
cord-007211-prygoc0q	71	29	purified	purify	VBN
cord-007211-prygoc0q	71	30	from	from	IN
cord-007211-prygoc0q	71	31	virions	virion	NNS
cord-007211-prygoc0q	71	32	by	by	IN
cord-007211-prygoc0q	71	33	SDS	sds	NN
cord-007211-prygoc0q	71	34	-	-	HYPH
cord-007211-prygoc0q	71	35	polyacrylamide	polyacrylamide	NN
cord-007211-prygoc0q	71	36	gel	gel	NN
cord-007211-prygoc0q	71	37	electrophoresis	electrophoresis	NN
cord-007211-prygoc0q	71	38	(	(	-LRB-
cord-007211-prygoc0q	71	39	SDS	SDS	NNP
cord-007211-prygoc0q	71	40	-	-	HYPH
cord-007211-prygoc0q	71	41	PAGE	PAGE	NNP
cord-007211-prygoc0q	71	42	)	)	-RRB-
cord-007211-prygoc0q	71	43	(	(	-LRB-
cord-007211-prygoc0q	71	44	23	23	CD
cord-007211-prygoc0q	71	45	)	)	-RRB-
cord-007211-prygoc0q	71	46	.	.	.
cord-007211-prygoc0q	72	1	Anti	Anti	NNP
cord-007211-prygoc0q	72	2	-	-	NNP
cord-007211-prygoc0q	72	3	HN	HN	NNP
cord-007211-prygoc0q	72	4	serum	serum	NN
cord-007211-prygoc0q	72	5	used	use	VBN
cord-007211-prygoc0q	72	6	to	to	TO
cord-007211-prygoc0q	72	7	detect	detect	VB
cord-007211-prygoc0q	72	8	HN	HN	NNP
cord-007211-prygoc0q	72	9	protein	protein	NN
cord-007211-prygoc0q	72	10	by	by	IN
cord-007211-prygoc0q	72	11	Western	western	JJ
cord-007211-prygoc0q	72	12	blotting	blotting	NN
cord-007211-prygoc0q	72	13	was	be	VBD
cord-007211-prygoc0q	72	14	obtained	obtain	VBN
cord-007211-prygoc0q	72	15	from	from	IN
cord-007211-prygoc0q	72	16	rabbits	rabbit	NNS
cord-007211-prygoc0q	72	17	immunized	immunize	VBN
cord-007211-prygoc0q	72	18	with	with	IN
cord-007211-prygoc0q	72	19	the	the	DT
cord-007211-prygoc0q	72	20	HN	HN	NNP
cord-007211-prygoc0q	72	21	protein	protein	NN
cord-007211-prygoc0q	72	22	,	,	,
cord-007211-prygoc0q	72	23	which	which	WDT
cord-007211-prygoc0q	72	24	was	be	VBD
cord-007211-prygoc0q	72	25	purified	purify	VBN
cord-007211-prygoc0q	72	26	from	from	IN
cord-007211-prygoc0q	72	27	virions	virion	NNS
cord-007211-prygoc0q	72	28	by	by	IN
cord-007211-prygoc0q	72	29	SDS	SDS	NNP
cord-007211-prygoc0q	72	30	-	-	HYPH
cord-007211-prygoc0q	72	31	PAGE	PAGE	NNP
cord-007211-prygoc0q	72	32	.	.	.
cord-007211-prygoc0q	73	1	Monoclonal	monoclonal	JJ
cord-007211-prygoc0q	73	2	antibodies	antibody	NNS
cord-007211-prygoc0q	73	3	(	(	-LRB-
cord-007211-prygoc0q	73	4	MAbs	MAbs	NNP
cord-007211-prygoc0q	73	5	)	)	-RRB-
cord-007211-prygoc0q	73	6	f-49	f-49	NNP
cord-007211-prygoc0q	73	7	,	,	,
cord-007211-prygoc0q	73	8	f-236	f-236	NNP
cord-007211-prygoc0q	73	9	,	,	,
cord-007211-prygoc0q	73	10	f-921	f-921	NNS
cord-007211-prygoc0q	73	11	,	,	,
cord-007211-prygoc0q	73	12	and	and	CC
cord-007211-prygoc0q	73	13	yl-111	yl-111	NNP
cord-007211-prygoc0q	73	14	were	be	VBD
cord-007211-prygoc0q	73	15	the	the	DT
cord-007211-prygoc0q	73	16	generous	generous	JJ
cord-007211-prygoc0q	73	17	gift	gift	NN
cord-007211-prygoc0q	73	18	of	of	IN
cord-007211-prygoc0q	73	19	Dr.	Dr.	NNP
cord-007211-prygoc0q	73	20	H.	H.	NNP
cord-007211-prygoc0q	73	21	Tozawa	Tozawa	NNP
cord-007211-prygoc0q	73	22	(	(	-LRB-
cord-007211-prygoc0q	73	23	Kitasato	Kitasato	NNP
cord-007211-prygoc0q	73	24	University	University	NNP
cord-007211-prygoc0q	73	25	)	)	-RRB-
cord-007211-prygoc0q	73	26	.	.	.
cord-007211-prygoc0q	74	1	MAb	MAb	NNPS
cord-007211-prygoc0q	74	2	f-49	f-49	NNP
cord-007211-prygoc0q	74	3	reacts	react	VBZ
cord-007211-prygoc0q	74	4	with	with	IN
cord-007211-prygoc0q	74	5	the	the	DT
cord-007211-prygoc0q	74	6	mature	mature	JJ
cord-007211-prygoc0q	74	7	form	form	NN
cord-007211-prygoc0q	74	8	of	of	IN
cord-007211-prygoc0q	74	9	F	F	NNP
cord-007211-prygoc0q	74	10	protein	protein	NN
cord-007211-prygoc0q	74	11	(	(	-LRB-
cord-007211-prygoc0q	74	12	24	24	CD
cord-007211-prygoc0q	74	13	)	)	-RRB-
cord-007211-prygoc0q	74	14	,	,	,
cord-007211-prygoc0q	74	15	and	and	CC
cord-007211-prygoc0q	74	16	f-236	f-236	NN
cord-007211-prygoc0q	74	17	recognizes	recognize	VBZ
cord-007211-prygoc0q	74	18	both	both	CC
cord-007211-prygoc0q	74	19	F	F	NNP
cord-007211-prygoc0q	74	20	o	o	NN
cord-007211-prygoc0q	74	21	and	and	CC
cord-007211-prygoc0q	74	22	F	F	NNP
cord-007211-prygoc0q	74	23	!	!	.
cord-007211-prygoc0q	75	1	in	in	IN
cord-007211-prygoc0q	75	2	immunoprecipitation	immunoprecipitation	NN
cord-007211-prygoc0q	75	3	but	but	CC
cord-007211-prygoc0q	75	4	reacts	react	VBZ
cord-007211-prygoc0q	75	5	specifically	specifically	RB
cord-007211-prygoc0q	75	6	with	with	IN
cord-007211-prygoc0q	75	7	F	F	NNP
cord-007211-prygoc0q	75	8	o	o	NNP
cord-007211-prygoc0q	75	9	in	in	IN
cord-007211-prygoc0q	75	10	Western	western	JJ
cord-007211-prygoc0q	75	11	blot	blot	NN
cord-007211-prygoc0q	75	12	analysis	analysis	NN
cord-007211-prygoc0q	75	13	.	.	.
cord-007211-prygoc0q	76	1	MAb	MAb	NNS
cord-007211-prygoc0q	76	2	f-921	f-921	NNS
cord-007211-prygoc0q	76	3	reacts	react	VBZ
cord-007211-prygoc0q	76	4	with	with	IN
cord-007211-prygoc0q	76	5	the	the	DT
cord-007211-prygoc0q	76	6	immature	immature	JJ
cord-007211-prygoc0q	76	7	form	form	NN
cord-007211-prygoc0q	76	8	of	of	IN
cord-007211-prygoc0q	76	9	F	F	NNP
cord-007211-prygoc0q	76	10	protein	protein	NN
cord-007211-prygoc0q	76	11	.	.	.
cord-007211-prygoc0q	77	1	In	in	IN
cord-007211-prygoc0q	77	2	a	a	DT
cord-007211-prygoc0q	77	3	competitive	competitive	JJ
cord-007211-prygoc0q	77	4	binding	binding	NN
cord-007211-prygoc0q	77	5	assay	assay	NN
cord-007211-prygoc0q	77	6	,	,	,
cord-007211-prygoc0q	77	7	the	the	DT
cord-007211-prygoc0q	77	8	MAbs	MAbs	NNP
cord-007211-prygoc0q	77	9	were	be	VBD
cord-007211-prygoc0q	77	10	divided	divide	VBN
cord-007211-prygoc0q	77	11	into	into	IN
cord-007211-prygoc0q	77	12	two	two	CD
cord-007211-prygoc0q	77	13	groups	group	NNS
cord-007211-prygoc0q	77	14	,	,	,
cord-007211-prygoc0q	77	15	F	F	NNP
cord-007211-prygoc0q	77	16	-	-	HYPH
cord-007211-prygoc0q	77	17	I	I	NNP
cord-007211-prygoc0q	77	18	and	and	CC
cord-007211-prygoc0q	77	19	F	F	NNP
cord-007211-prygoc0q	77	20	-	-	HYPH
cord-007211-prygoc0q	77	21	II	II	NNP
cord-007211-prygoc0q	77	22	.	.	.
cord-007211-prygoc0q	78	1	MAbs	MAbs	NNP
cord-007211-prygoc0q	78	2	f-236	f-236	RB
cord-007211-prygoc0q	79	1	and	and	CC
cord-007211-prygoc0q	79	2	f-921	f-921	NNS
cord-007211-prygoc0q	79	3	belonged	belong	VBD
cord-007211-prygoc0q	79	4	to	to	IN
cord-007211-prygoc0q	79	5	the	the	DT
cord-007211-prygoc0q	79	6	F	F	NNP
cord-007211-prygoc0q	79	7	-	-	HYPH
cord-007211-prygoc0q	79	8	I	I	NNP
cord-007211-prygoc0q	79	9	group	group	NN
cord-007211-prygoc0q	79	10	and	and	CC
cord-007211-prygoc0q	79	11	f-49	f-49	NNP
cord-007211-prygoc0q	79	12	belonged	belong	VBD
cord-007211-prygoc0q	79	13	to	to	IN
cord-007211-prygoc0q	79	14	the	the	DT
cord-007211-prygoc0q	79	15	F	F	NNP
cord-007211-prygoc0q	79	16	-	-	HYPH
cord-007211-prygoc0q	79	17	II	II	NNP
cord-007211-prygoc0q	79	18	group	group	NN
cord-007211-prygoc0q	79	19	(	(	-LRB-
cord-007211-prygoc0q	79	20	25	25	CD
cord-007211-prygoc0q	79	21	)	)	-RRB-
cord-007211-prygoc0q	79	22	.	.	.
cord-007211-prygoc0q	80	1	MAb	MAb	NNS
cord-007211-prygoc0q	81	1	yl-111	yl-111	NNP
cord-007211-prygoc0q	81	2	recognizes	recognize	VBZ
cord-007211-prygoc0q	81	3	the	the	DT
cord-007211-prygoc0q	81	4	HN	HN	NNP
cord-007211-prygoc0q	81	5	protein	protein	NN
cord-007211-prygoc0q	81	6	.	.	.
cord-007211-prygoc0q	82	1	Indirect	indirect	JJ
cord-007211-prygoc0q	82	2	Immunofluorescence	Immunofluorescence	NNP
cord-007211-prygoc0q	82	3	Staining	staining	NN
cord-007211-prygoc0q	82	4	-	-	HYPH
cord-007211-prygoc0q	82	5	Indirect	indirect	JJ
cord-007211-prygoc0q	82	6	immunofluorescence	immunofluorescence	NN
cord-007211-prygoc0q	82	7	staining	staining	NN
cord-007211-prygoc0q	82	8	was	be	VBD
cord-007211-prygoc0q	82	9	performed	perform	VBN
cord-007211-prygoc0q	82	10	as	as	IN
cord-007211-prygoc0q	82	11	described	describe	VBN
cord-007211-prygoc0q	82	12	previously	previously	RB
cord-007211-prygoc0q	82	13	(	(	-LRB-
cord-007211-prygoc0q	82	14	26	26	CD
cord-007211-prygoc0q	82	15	)	)	-RRB-
cord-007211-prygoc0q	82	16	.	.	.
cord-007211-prygoc0q	83	1	In	in	IN
cord-007211-prygoc0q	83	2	general	general	JJ
cord-007211-prygoc0q	83	3	,	,	,
cord-007211-prygoc0q	83	4	COS	cos	NN
cord-007211-prygoc0q	83	5	cells	cell	NNS
cord-007211-prygoc0q	83	6	were	be	VBD
cord-007211-prygoc0q	83	7	grown	grow	VBN
cord-007211-prygoc0q	83	8	on	on	IN
cord-007211-prygoc0q	83	9	glass	glass	NN
cord-007211-prygoc0q	83	10	coverslips	coverslip	NNS
cord-007211-prygoc0q	83	11	and	and	CC
cord-007211-prygoc0q	83	12	transfected	transfecte	VBN
cord-007211-prygoc0q	83	13	with	with	IN
cord-007211-prygoc0q	83	14	plasmid	plasmid	NN
cord-007211-prygoc0q	83	15	DNA	dna	NN
cord-007211-prygoc0q	83	16	as	as	IN
cord-007211-prygoc0q	83	17	described	describe	VBN
cord-007211-prygoc0q	83	18	above	above	RB
cord-007211-prygoc0q	83	19	.	.	.
cord-007211-prygoc0q	84	1	Forty	forty	CD
cord-007211-prygoc0q	84	2	-	-	HYPH
cord-007211-prygoc0q	84	3	eight	eight	CD
cord-007211-prygoc0q	84	4	hours	hour	NNS
cord-007211-prygoc0q	84	5	after	after	IN
cord-007211-prygoc0q	84	6	transfection	transfection	NN
cord-007211-prygoc0q	84	7	,	,	,
cord-007211-prygoc0q	84	8	cells	cell	NNS
cord-007211-prygoc0q	84	9	were	be	VBD
cord-007211-prygoc0q	84	10	washed	wash	VBN
cord-007211-prygoc0q	84	11	with	with	IN
cord-007211-prygoc0q	84	12	ice	ice	NN
cord-007211-prygoc0q	84	13	-	-	HYPH
cord-007211-prygoc0q	84	14	cold	cold	JJ
cord-007211-prygoc0q	84	15	phosphate	phosphate	NN
cord-007211-prygoc0q	84	16	buffered	buffer	VBN
cord-007211-prygoc0q	84	17	saline	saline	NN
cord-007211-prygoc0q	84	18	(	(	-LRB-
cord-007211-prygoc0q	84	19	PBS	PBS	NNP
cord-007211-prygoc0q	84	20	)	)	-RRB-
cord-007211-prygoc0q	84	21	and	and	CC
cord-007211-prygoc0q	84	22	fixed	fix	VBN
cord-007211-prygoc0q	84	23	with	with	IN
cord-007211-prygoc0q	84	24	acetone	acetone	NN
cord-007211-prygoc0q	84	25	for	for	IN
cord-007211-prygoc0q	84	26	10	10	CD
cord-007211-prygoc0q	84	27	min	min	NN
cord-007211-prygoc0q	84	28	at	at	IN
cord-007211-prygoc0q	84	29	-20	-20	NNP
cord-007211-prygoc0q	84	30	°	°	NNP
cord-007211-prygoc0q	84	31	C	C	NNP
cord-007211-prygoc0q	84	32	or	or	CC
cord-007211-prygoc0q	84	33	with	with	IN
cord-007211-prygoc0q	84	34	3.7	3.7	CD
cord-007211-prygoc0q	84	35	%	%	NN
cord-007211-prygoc0q	84	36	paraformaldehyde	paraformaldehyde	NNP
cord-007211-prygoc0q	84	37	in	in	IN
cord-007211-prygoc0q	84	38	100	100	CD
cord-007211-prygoc0q	84	39	mM	mm	CD
cord-007211-prygoc0q	84	40	sodium	sodium	NN
cord-007211-prygoc0q	84	41	phosphate	phosphate	NN
cord-007211-prygoc0q	84	42	buffer	buffer	NN
cord-007211-prygoc0q	84	43	(	(	-LRB-
cord-007211-prygoc0q	84	44	pH	pH	NNP
cord-007211-prygoc0q	84	45	6.5	6.5	CD
cord-007211-prygoc0q	84	46	)	)	-RRB-
cord-007211-prygoc0q	84	47	for	for	IN
cord-007211-prygoc0q	84	48	30	30	CD
cord-007211-prygoc0q	84	49	min	min	NN
cord-007211-prygoc0q	84	50	at	at	IN
cord-007211-prygoc0q	84	51	room	room	NN
cord-007211-prygoc0q	84	52	temperature	temperature	NN
cord-007211-prygoc0q	84	53	.	.	.
cord-007211-prygoc0q	85	1	The	the	DT
cord-007211-prygoc0q	85	2	cells	cell	NNS
cord-007211-prygoc0q	85	3	were	be	VBD
cord-007211-prygoc0q	85	4	washed	wash	VBN
cord-007211-prygoc0q	85	5	twice	twice	RB
cord-007211-prygoc0q	85	6	with	with	IN
cord-007211-prygoc0q	85	7	PBS	PBS	NNP
cord-007211-prygoc0q	85	8	for	for	IN
cord-007211-prygoc0q	85	9	10	10	CD
cord-007211-prygoc0q	85	10	min	min	NN
cord-007211-prygoc0q	85	11	,	,	,
cord-007211-prygoc0q	85	12	then	then	RB
cord-007211-prygoc0q	85	13	incubated	incubate	VBD
cord-007211-prygoc0q	85	14	for	for	IN
cord-007211-prygoc0q	85	15	1	1	CD
cord-007211-prygoc0q	85	16	h	h	NN
cord-007211-prygoc0q	85	17	at	at	IN
cord-007211-prygoc0q	85	18	37	37	CD
cord-007211-prygoc0q	85	19	°	°	NN
cord-007211-prygoc0q	85	20	C	C	NNP
cord-007211-prygoc0q	85	21	in	in	IN
cord-007211-prygoc0q	85	22	PBS	PBS	NNP
cord-007211-prygoc0q	85	23	containing	contain	VBG
cord-007211-prygoc0q	85	24	3	3	CD
cord-007211-prygoc0q	85	25	%	%	NN
cord-007211-prygoc0q	85	26	BSA	BSA	NNP
cord-007211-prygoc0q	85	27	,	,	,
cord-007211-prygoc0q	85	28	0.02	0.02	CD
cord-007211-prygoc0q	85	29	%	%	NN
cord-007211-prygoc0q	85	30	NaN	NaN	NNP
cord-007211-prygoc0q	85	31	3	3	CD
cord-007211-prygoc0q	85	32	,	,	,
cord-007211-prygoc0q	85	33	and	and	CC
cord-007211-prygoc0q	85	34	anti	anti	JJ
cord-007211-prygoc0q	85	35	-	-	JJ
cord-007211-prygoc0q	85	36	F	f	JJ
cord-007211-prygoc0q	85	37	rabbit	rabbit	NN
cord-007211-prygoc0q	85	38	serum	serum	NN
cord-007211-prygoc0q	85	39	or	or	CC
cord-007211-prygoc0q	85	40	yl-111	yl-111	NNP
cord-007211-prygoc0q	85	41	(	(	-LRB-
cord-007211-prygoc0q	85	42	diluted	diluted	JJ
cord-007211-prygoc0q	85	43	1:100	1:100	CD
cord-007211-prygoc0q	85	44	)	)	-RRB-
cord-007211-prygoc0q	85	45	.	.	.
cord-007211-prygoc0q	86	1	After	after	IN
cord-007211-prygoc0q	86	2	incubation	incubation	NN
cord-007211-prygoc0q	86	3	with	with	IN
cord-007211-prygoc0q	86	4	the	the	DT
cord-007211-prygoc0q	86	5	antiserum	antiserum	NN
cord-007211-prygoc0q	86	6	,	,	,
cord-007211-prygoc0q	86	7	cells	cell	NNS
cord-007211-prygoc0q	86	8	were	be	VBD
cord-007211-prygoc0q	86	9	washed	wash	VBN
cord-007211-prygoc0q	86	10	with	with	IN
cord-007211-prygoc0q	86	11	PBS	PBS	NNP
cord-007211-prygoc0q	86	12	twice	twice	RB
cord-007211-prygoc0q	86	13	,	,	,
cord-007211-prygoc0q	86	14	then	then	RB
cord-007211-prygoc0q	86	15	incubated	incubate	VBN
cord-007211-prygoc0q	86	16	for	for	IN
cord-007211-prygoc0q	86	17	1	1	CD
cord-007211-prygoc0q	86	18	h	h	NN
cord-007211-prygoc0q	86	19	at	at	IN
cord-007211-prygoc0q	86	20	37	37	CD
cord-007211-prygoc0q	86	21	°	°	NN
cord-007211-prygoc0q	86	22	C	C	NNP
cord-007211-prygoc0q	86	23	in	in	IN
cord-007211-prygoc0q	86	24	PBS	PBS	NNP
cord-007211-prygoc0q	86	25	containing	contain	VBG
cord-007211-prygoc0q	86	26	NaN	NaN	NNP
cord-007211-prygoc0q	86	27	3	3	CD
cord-007211-prygoc0q	86	28	,	,	,
cord-007211-prygoc0q	86	29	BSA	BSA	NNP
cord-007211-prygoc0q	86	30	,	,	,
cord-007211-prygoc0q	86	31	and	and	CC
cord-007211-prygoc0q	86	32	fluorescein	fluorescein	NN
cord-007211-prygoc0q	86	33	isothiocyanate	isothiocyanate	NN
cord-007211-prygoc0q	86	34	(	(	-LRB-
cord-007211-prygoc0q	86	35	FITC)-conjugated	FITC)-conjugated	NNP
cord-007211-prygoc0q	86	36	anti	anti	JJ
cord-007211-prygoc0q	86	37	-	-	JJ
cord-007211-prygoc0q	86	38	rabbit	rabbit	JJ
cord-007211-prygoc0q	86	39	IgG	igg	NN
cord-007211-prygoc0q	86	40	or	or	CC
cord-007211-prygoc0q	86	41	anti	anti	JJ
cord-007211-prygoc0q	86	42	-	-	JJ
cord-007211-prygoc0q	86	43	mouse	mouse	JJ
cord-007211-prygoc0q	86	44	IgG	igg	NN
cord-007211-prygoc0q	86	45	(	(	-LRB-
cord-007211-prygoc0q	86	46	Wako	Wako	NNP
cord-007211-prygoc0q	86	47	Pure	Pure	NNP
cord-007211-prygoc0q	86	48	Chemical	Chemical	NNP
cord-007211-prygoc0q	86	49	Industries	Industries	NNPS
cord-007211-prygoc0q	86	50	)	)	-RRB-
cord-007211-prygoc0q	86	51	,	,	,
cord-007211-prygoc0q	86	52	each	each	DT
cord-007211-prygoc0q	86	53	diluted	dilute	VBN
cord-007211-prygoc0q	86	54	1:100	1:100	CD
cord-007211-prygoc0q	86	55	,	,	,
cord-007211-prygoc0q	86	56	as	as	IN
cord-007211-prygoc0q	86	57	secondary	secondary	JJ
cord-007211-prygoc0q	86	58	antibody	antibody	NN
cord-007211-prygoc0q	86	59	to	to	IN
cord-007211-prygoc0q	86	60	anti	anti	JJ
cord-007211-prygoc0q	86	61	-	-	JJ
cord-007211-prygoc0q	86	62	F	f	JJ
cord-007211-prygoc0q	86	63	rabbit	rabbit	NN
cord-007211-prygoc0q	86	64	serum	serum	NN
cord-007211-prygoc0q	86	65	or	or	CC
cord-007211-prygoc0q	86	66	yl-111	yl-111	NN
cord-007211-prygoc0q	86	67	,	,	,
cord-007211-prygoc0q	86	68	respectively	respectively	RB
cord-007211-prygoc0q	86	69	.	.	.
cord-007211-prygoc0q	87	1	Immunofluorescence	Immunofluorescence	NNP
cord-007211-prygoc0q	87	2	was	be	VBD
cord-007211-prygoc0q	87	3	examined	examine	VBN
cord-007211-prygoc0q	87	4	by	by	IN
cord-007211-prygoc0q	87	5	fluorescence	fluorescence	NN
cord-007211-prygoc0q	87	6	microscopy	microscopy	NN
cord-007211-prygoc0q	87	7	.	.	.
cord-007211-prygoc0q	88	1	Western	Western	NNP
cord-007211-prygoc0q	88	2	Blot	Blot	NNP
cord-007211-prygoc0q	88	3	Analysis	Analysis	NNP
cord-007211-prygoc0q	88	4	-	-	HYPH
cord-007211-prygoc0q	88	5	COS-1	COS-1	NNP
cord-007211-prygoc0q	88	6	cells	cell	NNS
cord-007211-prygoc0q	88	7	(	(	-LRB-
cord-007211-prygoc0q	88	8	in	in	IN
cord-007211-prygoc0q	88	9	12-well	12-well	CD
cord-007211-prygoc0q	88	10	dishes	dish	NNS
cord-007211-prygoc0q	88	11	)	)	-RRB-
cord-007211-prygoc0q	88	12	were	be	VBD
cord-007211-prygoc0q	88	13	transfected	transfecte	VBN
cord-007211-prygoc0q	88	14	with	with	IN
cord-007211-prygoc0q	88	15	plasmid	plasmid	NN
cord-007211-prygoc0q	88	16	DNA	dna	NN
cord-007211-prygoc0q	88	17	as	as	IN
cord-007211-prygoc0q	88	18	described	describe	VBN
cord-007211-prygoc0q	88	19	above	above	RB
cord-007211-prygoc0q	88	20	.	.	.
cord-007211-prygoc0q	89	1	Forty	forty	CD
cord-007211-prygoc0q	89	2	-	-	HYPH
cord-007211-prygoc0q	89	3	eight	eight	CD
cord-007211-prygoc0q	89	4	hours	hour	NNS
cord-007211-prygoc0q	89	5	after	after	IN
cord-007211-prygoc0q	89	6	transfection	transfection	NN
cord-007211-prygoc0q	89	7	,	,	,
cord-007211-prygoc0q	89	8	cells	cell	NNS
cord-007211-prygoc0q	89	9	were	be	VBD
cord-007211-prygoc0q	89	10	washed	wash	VBN
cord-007211-prygoc0q	89	11	with	with	IN
cord-007211-prygoc0q	89	12	ice	ice	NN
cord-007211-prygoc0q	89	13	-	-	HYPH
cord-007211-prygoc0q	89	14	cold	cold	JJ
cord-007211-prygoc0q	89	15	PBS	PBS	NNP
cord-007211-prygoc0q	89	16	containing	contain	VBG
cord-007211-prygoc0q	89	17	20	20	CD
cord-007211-prygoc0q	89	18	mM	mm	NN
cord-007211-prygoc0q	89	19	iodoacetamide	iodoacetamide	NN
cord-007211-prygoc0q	89	20	(	(	-LRB-
cord-007211-prygoc0q	89	21	IAA	IAA	NNP
cord-007211-prygoc0q	89	22	)	)	-RRB-
cord-007211-prygoc0q	89	23	,	,	,
cord-007211-prygoc0q	89	24	lysed	lyse	VBN
cord-007211-prygoc0q	89	25	in	in	IN
cord-007211-prygoc0q	89	26	100	100	CD
cord-007211-prygoc0q	89	27	A	A	NNP
cord-007211-prygoc0q	89	28	l	l	NN
cord-007211-prygoc0q	89	29	of	of	IN
cord-007211-prygoc0q	89	30	RIPA	RIPA	NNP
cord-007211-prygoc0q	89	31	buffer	buffer	NN
cord-007211-prygoc0q	89	32	[	[	-LRB-
cord-007211-prygoc0q	89	33	0.01	0.01	CD
cord-007211-prygoc0q	89	34	M	M	NNP
cord-007211-prygoc0q	89	35	Tris	Tris	NNP
cord-007211-prygoc0q	89	36	-	-	HYPH
cord-007211-prygoc0q	89	37	HCl	HCl	NNP
cord-007211-prygoc0q	89	38	(	(	-LRB-
cord-007211-prygoc0q	89	39	pH	pH	NNP
cord-007211-prygoc0q	89	40	7.5	7.5	CD
cord-007211-prygoc0q	89	41	)	)	-RRB-
cord-007211-prygoc0q	89	42	,	,	,
cord-007211-prygoc0q	89	43	0.15	0.15	CD
cord-007211-prygoc0q	89	44	M	M	NNP
cord-007211-prygoc0q	89	45	NaCl	NaCl	NNP
cord-007211-prygoc0q	89	46	,	,	,
cord-007211-prygoc0q	89	47	1	1	CD
cord-007211-prygoc0q	89	48	%	%	NN
cord-007211-prygoc0q	89	49	Triton	Triton	NNP
cord-007211-prygoc0q	89	50	X-100	x-100	NN
cord-007211-prygoc0q	89	51	,	,	,
cord-007211-prygoc0q	89	52	1	1	CD
cord-007211-prygoc0q	89	53	%	%	NN
cord-007211-prygoc0q	89	54	sodium	sodium	NN
cord-007211-prygoc0q	89	55	deoxycholate	deoxycholate	NN
cord-007211-prygoc0q	89	56	,	,	,
cord-007211-prygoc0q	89	57	0.1	0.1	CD
cord-007211-prygoc0q	89	58	%	%	NN
cord-007211-prygoc0q	89	59	SDS	SDS	NNP
cord-007211-prygoc0q	89	60	]	]	-RRB-
cord-007211-prygoc0q	89	61	containing	contain	VBG
cord-007211-prygoc0q	89	62	2	2	CD
cord-007211-prygoc0q	89	63	mM	mm	CD
cord-007211-prygoc0q	89	64	PMSF	PMSF	NNP
cord-007211-prygoc0q	89	65	and	and	CC
cord-007211-prygoc0q	89	66	20	20	CD
cord-007211-prygoc0q	89	67	mM	mm	CD
cord-007211-prygoc0q	89	68	IAA	IAA	NNP
cord-007211-prygoc0q	89	69	,	,	,
cord-007211-prygoc0q	89	70	and	and	CC
cord-007211-prygoc0q	89	71	sonicated	sonicate	VBN
cord-007211-prygoc0q	89	72	for	for	IN
cord-007211-prygoc0q	89	73	30	30	CD
cord-007211-prygoc0q	89	74	s.	s.	NNP
cord-007211-prygoc0q	89	75	Twenty	Twenty	NNP
cord-007211-prygoc0q	89	76	-	-	HYPH
cord-007211-prygoc0q	89	77	five	five	CD
cord-007211-prygoc0q	89	78	microliters	microliter	NNS
cord-007211-prygoc0q	89	79	of	of	IN
cord-007211-prygoc0q	89	80	5xSDS	5xsds	CD
cord-007211-prygoc0q	89	81	sample	sample	NN
cord-007211-prygoc0q	89	82	buffer	buffer	NN
cord-007211-prygoc0q	89	83	[	[	-LRB-
cord-007211-prygoc0q	89	84	312.5	312.5	CD
cord-007211-prygoc0q	89	85	mM	mM	NNP
cord-007211-prygoc0q	89	86	Tris	Tris	NNP
cord-007211-prygoc0q	89	87	-	-	HYPH
cord-007211-prygoc0q	89	88	HCl	HCl	NNP
cord-007211-prygoc0q	89	89	(	(	-LRB-
cord-007211-prygoc0q	89	90	pH	pH	NNP
cord-007211-prygoc0q	89	91	6.8	6.8	CD
cord-007211-prygoc0q	89	92	)	)	-RRB-
cord-007211-prygoc0q	89	93	,	,	,
cord-007211-prygoc0q	89	94	25	25	CD
cord-007211-prygoc0q	89	95	%	%	NN
cord-007211-prygoc0q	89	96	2-mercaptoethanol	2-mercaptoethanol	CD
cord-007211-prygoc0q	89	97	(	(	-LRB-
cord-007211-prygoc0q	89	98	2-ME	2-me	CD
cord-007211-prygoc0q	89	99	)	)	-RRB-
cord-007211-prygoc0q	89	100	,	,	,
cord-007211-prygoc0q	89	101	50	50	CD
cord-007211-prygoc0q	89	102	%	%	NN
cord-007211-prygoc0q	89	103	glycerol	glycerol	NN
cord-007211-prygoc0q	89	104	,	,	,
cord-007211-prygoc0q	89	105	10	10	CD
cord-007211-prygoc0q	89	106	%	%	NN
cord-007211-prygoc0q	89	107	SDS	SDS	NNP
cord-007211-prygoc0q	89	108	,	,	,
cord-007211-prygoc0q	89	109	0.05	0.05	CD
cord-007211-prygoc0q	89	110	%	%	NN
cord-007211-prygoc0q	89	111	Bromophenol	Bromophenol	NNP
cord-007211-prygoc0q	89	112	blue	blue	NN
cord-007211-prygoc0q	89	113	]	]	-RRB-
cord-007211-prygoc0q	89	114	was	be	VBD
cord-007211-prygoc0q	89	115	added	add	VBN
cord-007211-prygoc0q	89	116	to	to	IN
cord-007211-prygoc0q	89	117	the	the	DT
cord-007211-prygoc0q	89	118	cell	cell	NN
cord-007211-prygoc0q	89	119	lysates	lysate	NNS
cord-007211-prygoc0q	89	120	and	and	CC
cord-007211-prygoc0q	89	121	boiled	boil	VBN
cord-007211-prygoc0q	89	122	for	for	IN
cord-007211-prygoc0q	89	123	3	3	CD
cord-007211-prygoc0q	89	124	min	min	NN
cord-007211-prygoc0q	89	125	.	.	.
cord-007211-prygoc0q	90	1	Fifteen	fifteen	CD
cord-007211-prygoc0q	90	2	microliters	microliter	NNS
cord-007211-prygoc0q	90	3	of	of	IN
cord-007211-prygoc0q	90	4	each	each	DT
cord-007211-prygoc0q	90	5	sample	sample	NN
cord-007211-prygoc0q	90	6	was	be	VBD
cord-007211-prygoc0q	90	7	electrophoresed	electrophoresed	JJ
cord-007211-prygoc0q	90	8	on	on	IN
cord-007211-prygoc0q	90	9	a	a	DT
cord-007211-prygoc0q	90	10	9	9	CD
cord-007211-prygoc0q	90	11	%	%	NN
cord-007211-prygoc0q	90	12	polyacrylamide	polyacrylamide	NN
cord-007211-prygoc0q	90	13	gel	gel	NN
cord-007211-prygoc0q	90	14	.	.	.
cord-007211-prygoc0q	91	1	Proteins	protein	NNS
cord-007211-prygoc0q	91	2	were	be	VBD
cord-007211-prygoc0q	91	3	transferred	transfer	VBN
cord-007211-prygoc0q	91	4	to	to	IN
cord-007211-prygoc0q	91	5	Immobilon	Immobilon	NNP
cord-007211-prygoc0q	91	6	-	-	HYPH
cord-007211-prygoc0q	91	7	P	p	NN
cord-007211-prygoc0q	91	8	membrane	membrane	NN
cord-007211-prygoc0q	91	9	(	(	-LRB-
cord-007211-prygoc0q	91	10	Millipore	Millipore	NNP
cord-007211-prygoc0q	91	11	)	)	-RRB-
cord-007211-prygoc0q	91	12	by	by	IN
cord-007211-prygoc0q	91	13	semi	semi	JJ
cord-007211-prygoc0q	91	14	-	-	JJ
cord-007211-prygoc0q	91	15	dry	dry	JJ
cord-007211-prygoc0q	91	16	electroblotting	electroblotting	NN
cord-007211-prygoc0q	91	17	.	.	.
cord-007211-prygoc0q	92	1	The	the	DT
cord-007211-prygoc0q	92	2	membrane	membrane	NN
cord-007211-prygoc0q	92	3	was	be	VBD
cord-007211-prygoc0q	92	4	preincubated	preincubate	VBN
cord-007211-prygoc0q	92	5	in	in	IN
cord-007211-prygoc0q	92	6	Trisbuffered	Trisbuffered	NNP
cord-007211-prygoc0q	92	7	saline	saline	NN
cord-007211-prygoc0q	92	8	(	(	-LRB-
cord-007211-prygoc0q	92	9	TBS	TBS	NNP
cord-007211-prygoc0q	92	10	)	)	-RRB-
cord-007211-prygoc0q	92	11	[	[	-LRB-
cord-007211-prygoc0q	92	12	0.02	0.02	CD
cord-007211-prygoc0q	92	13	M	M	NNP
cord-007211-prygoc0q	92	14	Tris	Tris	NNP
cord-007211-prygoc0q	92	15	-	-	HYPH
cord-007211-prygoc0q	92	16	HCl	HCl	NNP
cord-007211-prygoc0q	92	17	(	(	-LRB-
cord-007211-prygoc0q	92	18	pH	pH	NNP
cord-007211-prygoc0q	92	19	7.6	7.6	CD
cord-007211-prygoc0q	92	20	)	)	-RRB-
cord-007211-prygoc0q	92	21	,	,	,
cord-007211-prygoc0q	92	22	0.137	0.137	CD
cord-007211-prygoc0q	92	23	M	M	NNP
cord-007211-prygoc0q	92	24	NaCl	NaCl	NNP
cord-007211-prygoc0q	92	25	]	]	-RRB-
cord-007211-prygoc0q	92	26	containing	contain	VBG
cord-007211-prygoc0q	92	27	0.05	0.05	CD
cord-007211-prygoc0q	92	28	%	%	NN
cord-007211-prygoc0q	92	29	Tween20	Tween20	NNP
cord-007211-prygoc0q	92	30	,	,	,
cord-007211-prygoc0q	92	31	5	5	CD
cord-007211-prygoc0q	92	32	%	%	NN
cord-007211-prygoc0q	92	33	low	low	JJ
cord-007211-prygoc0q	92	34	fat	fat	JJ
cord-007211-prygoc0q	92	35	milk	milk	NN
cord-007211-prygoc0q	92	36	for	for	IN
cord-007211-prygoc0q	92	37	1	1	CD
cord-007211-prygoc0q	92	38	h	h	NN
cord-007211-prygoc0q	92	39	at	at	IN
cord-007211-prygoc0q	92	40	room	room	NN
cord-007211-prygoc0q	92	41	temperature	temperature	NN
cord-007211-prygoc0q	92	42	.	.	.
cord-007211-prygoc0q	93	1	The	the	DT
cord-007211-prygoc0q	93	2	membrane	membrane	NN
cord-007211-prygoc0q	93	3	was	be	VBD
cord-007211-prygoc0q	93	4	then	then	RB
cord-007211-prygoc0q	93	5	washed	wash	VBN
cord-007211-prygoc0q	93	6	in	in	IN
cord-007211-prygoc0q	93	7	TBS	TBS	NNP
cord-007211-prygoc0q	93	8	containing	contain	VBG
cord-007211-prygoc0q	93	9	0.05	0.05	CD
cord-007211-prygoc0q	93	10	%	%	NN
cord-007211-prygoc0q	93	11	Tween20	Tween20	NNP
cord-007211-prygoc0q	93	12	,	,	,
cord-007211-prygoc0q	93	13	incubated	incubate	VBN
cord-007211-prygoc0q	93	14	for	for	IN
cord-007211-prygoc0q	93	15	1	1	CD
cord-007211-prygoc0q	93	16	h	h	NN
cord-007211-prygoc0q	93	17	at	at	IN
cord-007211-prygoc0q	93	18	room	room	NN
cord-007211-prygoc0q	93	19	temperature	temperature	NN
cord-007211-prygoc0q	93	20	in	in	IN
cord-007211-prygoc0q	93	21	TBS	TBS	NNP
cord-007211-prygoc0q	93	22	-	-	HYPH
cord-007211-prygoc0q	93	23	Tween	Tween	NNP
cord-007211-prygoc0q	93	24	buffer	buffer	NN
cord-007211-prygoc0q	93	25	containing	contain	VBG
cord-007211-prygoc0q	93	26	a	a	DT
cord-007211-prygoc0q	93	27	1:1,000	1:1,000	CD
cord-007211-prygoc0q	93	28	each	each	DT
cord-007211-prygoc0q	93	29	dilution	dilution	NN
cord-007211-prygoc0q	93	30	of	of	IN
cord-007211-prygoc0q	93	31	the	the	DT
cord-007211-prygoc0q	93	32	anti	anti	NNP
cord-007211-prygoc0q	93	33	-	-	JJ
cord-007211-prygoc0q	93	34	F	f	JJ
cord-007211-prygoc0q	93	35	and	and	CC
cord-007211-prygoc0q	93	36	anti	anti	JJ
cord-007211-prygoc0q	93	37	-	-	JJ
cord-007211-prygoc0q	93	38	HN	HN	NNP
cord-007211-prygoc0q	93	39	antisera	antisera	NNP
cord-007211-prygoc0q	93	40	,	,	,
cord-007211-prygoc0q	93	41	washed	wash	VBD
cord-007211-prygoc0q	93	42	twice	twice	RB
cord-007211-prygoc0q	93	43	for	for	IN
cord-007211-prygoc0q	93	44	30	30	CD
cord-007211-prygoc0q	93	45	min	min	NN
cord-007211-prygoc0q	93	46	in	in	IN
cord-007211-prygoc0q	93	47	TBS	TBS	NNP
cord-007211-prygoc0q	93	48	-	-	HYPH
cord-007211-prygoc0q	93	49	Tween	Tween	NNP
cord-007211-prygoc0q	93	50	,	,	,
cord-007211-prygoc0q	93	51	and	and	CC
cord-007211-prygoc0q	93	52	incubated	incubate	VBN
cord-007211-prygoc0q	93	53	for	for	IN
cord-007211-prygoc0q	93	54	1	1	CD
cord-007211-prygoc0q	93	55	h	h	NN
cord-007211-prygoc0q	93	56	at	at	IN
cord-007211-prygoc0q	93	57	room	room	NN
cord-007211-prygoc0q	93	58	temperature	temperature	NN
cord-007211-prygoc0q	93	59	in	in	IN
cord-007211-prygoc0q	93	60	TBS	TBS	NNP
cord-007211-prygoc0q	93	61	-	-	HYPH
cord-007211-prygoc0q	93	62	Tween	Tween	NNP
cord-007211-prygoc0q	93	63	containing	contain	VBG
cord-007211-prygoc0q	93	64	a	a	DT
cord-007211-prygoc0q	93	65	1:4,000	1:4,000	CD
cord-007211-prygoc0q	93	66	dilution	dilution	NN
cord-007211-prygoc0q	93	67	of	of	IN
cord-007211-prygoc0q	93	68	horseradish	horseradish	NN
cord-007211-prygoc0q	93	69	peroxidase	peroxidase	NN
cord-007211-prygoc0q	93	70	(	(	-LRB-
cord-007211-prygoc0q	93	71	HRP)-conjugated	hrp)-conjugate	VBN
cord-007211-prygoc0q	94	1	protein	protein	NNP
cord-007211-prygoc0q	94	2	A	A	NNP
cord-007211-prygoc0q	94	3	(	(	-LRB-
cord-007211-prygoc0q	94	4	E.	E.	NNP
cord-007211-prygoc0q	94	5	Y	Y	NNP
cord-007211-prygoc0q	94	6	Laboratories	Laboratories	NNP
cord-007211-prygoc0q	94	7	)	)	-RRB-
cord-007211-prygoc0q	94	8	.	.	.
cord-007211-prygoc0q	95	1	After	after	IN
cord-007211-prygoc0q	95	2	extensive	extensive	JJ
cord-007211-prygoc0q	95	3	washing	washing	NN
cord-007211-prygoc0q	95	4	of	of	IN
cord-007211-prygoc0q	95	5	the	the	DT
cord-007211-prygoc0q	95	6	membrane	membrane	NN
cord-007211-prygoc0q	95	7	,	,	,
cord-007211-prygoc0q	95	8	bound	bind	VBN
cord-007211-prygoc0q	95	9	antibodies	antibody	NNS
cord-007211-prygoc0q	95	10	were	be	VBD
cord-007211-prygoc0q	95	11	detected	detect	VBN
cord-007211-prygoc0q	95	12	using	use	VBG
cord-007211-prygoc0q	95	13	the	the	DT
cord-007211-prygoc0q	95	14	ECL	ECL	NNP
cord-007211-prygoc0q	95	15	Western	western	JJ
cord-007211-prygoc0q	95	16	blotting	blot	VBG
cord-007211-prygoc0q	95	17	detection	detection	NN
cord-007211-prygoc0q	95	18	reagent	reagent	NN
cord-007211-prygoc0q	95	19	system	system	NN
cord-007211-prygoc0q	95	20	(	(	-LRB-
cord-007211-prygoc0q	95	21	Amersham	Amersham	NNP
cord-007211-prygoc0q	95	22	)	)	-RRB-
cord-007211-prygoc0q	95	23	.	.	.
cord-007211-prygoc0q	96	1	Immunoprecipitation	Immunoprecipitation	NNP
cord-007211-prygoc0q	96	2	-	-	HYPH
cord-007211-prygoc0q	96	3	COS-1	COS-1	NNP
cord-007211-prygoc0q	96	4	cells	cell	NNS
cord-007211-prygoc0q	96	5	(	(	-LRB-
cord-007211-prygoc0q	96	6	in	in	IN
cord-007211-prygoc0q	96	7	35-mm	35-mm	CD
cord-007211-prygoc0q	96	8	dishes	dish	NNS
cord-007211-prygoc0q	96	9	)	)	-RRB-
cord-007211-prygoc0q	96	10	were	be	VBD
cord-007211-prygoc0q	96	11	transfected	transfecte	VBN
cord-007211-prygoc0q	96	12	with	with	IN
cord-007211-prygoc0q	96	13	plasmid	plasmid	NN
cord-007211-prygoc0q	96	14	DNA	dna	NN
cord-007211-prygoc0q	96	15	as	as	IN
cord-007211-prygoc0q	96	16	described	describe	VBN
cord-007211-prygoc0q	96	17	above	above	RB
cord-007211-prygoc0q	96	18	.	.	.
cord-007211-prygoc0q	97	1	Forty	forty	CD
cord-007211-prygoc0q	97	2	-	-	HYPH
cord-007211-prygoc0q	97	3	eight	eight	CD
cord-007211-prygoc0q	97	4	hours	hour	NNS
cord-007211-prygoc0q	97	5	after	after	IN
cord-007211-prygoc0q	97	6	transfection	transfection	NN
cord-007211-prygoc0q	97	7	,	,	,
cord-007211-prygoc0q	97	8	the	the	DT
cord-007211-prygoc0q	97	9	medium	medium	NN
cord-007211-prygoc0q	97	10	was	be	VBD
cord-007211-prygoc0q	97	11	replaced	replace	VBN
cord-007211-prygoc0q	97	12	with	with	IN
cord-007211-prygoc0q	97	13	methionine	methionine	NN
cord-007211-prygoc0q	97	14	-	-	HYPH
cord-007211-prygoc0q	97	15	and	and	CC
cord-007211-prygoc0q	97	16	cysteine	cysteine	NN
cord-007211-prygoc0q	97	17	-	-	HYPH
cord-007211-prygoc0q	97	18	free	free	JJ
cord-007211-prygoc0q	97	19	minimum	minimum	JJ
cord-007211-prygoc0q	97	20	essential	essential	JJ
cord-007211-prygoc0q	97	21	medium	medium	NN
cord-007211-prygoc0q	97	22	(	(	-LRB-
cord-007211-prygoc0q	97	23	MEM	MEM	NNP
cord-007211-prygoc0q	97	24	)	)	-RRB-
cord-007211-prygoc0q	97	25	.	.	.
cord-007211-prygoc0q	98	1	Twenty	twenty	CD
cord-007211-prygoc0q	98	2	minutes	minute	NNS
cord-007211-prygoc0q	98	3	later	later	RB
cord-007211-prygoc0q	98	4	,	,	,
cord-007211-prygoc0q	98	5	cells	cell	NNS
cord-007211-prygoc0q	98	6	were	be	VBD
cord-007211-prygoc0q	98	7	labeled	label	VBN
cord-007211-prygoc0q	98	8	with	with	IN
cord-007211-prygoc0q	98	9	100	100	CD
cord-007211-prygoc0q	98	10	ix\	ix\	NNS
cord-007211-prygoc0q	98	11	of	of	IN
cord-007211-prygoc0q	98	12	methionine	methionine	NN
cord-007211-prygoc0q	98	13	-	-	HYPH
cord-007211-prygoc0q	98	14	and	and	CC
cord-007211-prygoc0q	98	15	cysteine	cysteine	NN
cord-007211-prygoc0q	98	16	-	-	HYPH
cord-007211-prygoc0q	98	17	free	free	JJ
cord-007211-prygoc0q	98	18	MEM	mem	NN
cord-007211-prygoc0q	98	19	supplemented	supplement	VBN
cord-007211-prygoc0q	98	20	with	with	IN
cord-007211-prygoc0q	98	21	[	[	-LRB-
cord-007211-prygoc0q	98	22	35	35	CD
cord-007211-prygoc0q	98	23	S	s	NN
cord-007211-prygoc0q	98	24	]	]	-RRB-
cord-007211-prygoc0q	98	25	methionine	methionine	NN
cord-007211-prygoc0q	98	26	and	and	CC
cord-007211-prygoc0q	98	27	[	[	-LRB-
cord-007211-prygoc0q	98	28	35	35	CD
cord-007211-prygoc0q	98	29	S	s	NN
cord-007211-prygoc0q	98	30	]	]	-RRB-
cord-007211-prygoc0q	98	31	cysteine	cysteine	NN
cord-007211-prygoc0q	98	32	(	(	-LRB-
cord-007211-prygoc0q	98	33	200/^Ci	200/^Ci	NNP
cord-007211-prygoc0q	98	34	/	/	SYM
cord-007211-prygoc0q	98	35	ml	ml	NN
cord-007211-prygoc0q	98	36	,	,	,
cord-007211-prygoc0q	98	37	1,000	1,000	CD
cord-007211-prygoc0q	98	38	Ci	ci	NN
cord-007211-prygoc0q	98	39	/	/	SYM
cord-007211-prygoc0q	98	40	mmol	mmol	NN
cord-007211-prygoc0q	98	41	;	;	:
cord-007211-prygoc0q	98	42	Du	Du	NNP
cord-007211-prygoc0q	98	43	Pont	Pont	NNP
cord-007211-prygoc0q	98	44	/	/	SYM
cord-007211-prygoc0q	98	45	NEN	NEN	NNP
cord-007211-prygoc0q	98	46	)	)	-RRB-
cord-007211-prygoc0q	98	47	,	,	,
cord-007211-prygoc0q	98	48	and	and	CC
cord-007211-prygoc0q	98	49	labeling	labeling	NN
cord-007211-prygoc0q	98	50	was	be	VBD
cord-007211-prygoc0q	98	51	continued	continue	VBN
cord-007211-prygoc0q	98	52	for	for	IN
cord-007211-prygoc0q	98	53	10	10	CD
cord-007211-prygoc0q	98	54	min	min	NN
cord-007211-prygoc0q	98	55	.	.	.
cord-007211-prygoc0q	99	1	The	the	DT
cord-007211-prygoc0q	99	2	cells	cell	NNS
cord-007211-prygoc0q	99	3	were	be	VBD
cord-007211-prygoc0q	99	4	chased	chase	VBN
cord-007211-prygoc0q	99	5	with	with	IN
cord-007211-prygoc0q	99	6	DMEM	DMEM	NNP
cord-007211-prygoc0q	99	7	supplemented	supplement	VBN
cord-007211-prygoc0q	99	8	with	with	IN
cord-007211-prygoc0q	99	9	5	5	CD
cord-007211-prygoc0q	99	10	%	%	NN
cord-007211-prygoc0q	99	11	FBS	FBS	NNP
cord-007211-prygoc0q	99	12	,	,	,
cord-007211-prygoc0q	99	13	5	5	CD
cord-007211-prygoc0q	99	14	mM	mm	CD
cord-007211-prygoc0q	99	15	methionine	methionine	NN
cord-007211-prygoc0q	99	16	,	,	,
cord-007211-prygoc0q	99	17	and	and	CC
cord-007211-prygoc0q	99	18	5	5	CD
cord-007211-prygoc0q	99	19	mM	mm	CD
cord-007211-prygoc0q	99	20	cysteine	cysteine	NN
cord-007211-prygoc0q	99	21	for	for	IN
cord-007211-prygoc0q	99	22	the	the	DT
cord-007211-prygoc0q	99	23	time	time	NN
cord-007211-prygoc0q	99	24	indicated	indicate	VBN
cord-007211-prygoc0q	99	25	in	in	IN
cord-007211-prygoc0q	99	26	each	each	DT
cord-007211-prygoc0q	99	27	figure	figure	NN
cord-007211-prygoc0q	99	28	legend	legend	NN
cord-007211-prygoc0q	99	29	.	.	.
cord-007211-prygoc0q	100	1	Then	then	RB
cord-007211-prygoc0q	100	2	the	the	DT
cord-007211-prygoc0q	100	3	cells	cell	NNS
cord-007211-prygoc0q	100	4	were	be	VBD
cord-007211-prygoc0q	100	5	washed	wash	VBN
cord-007211-prygoc0q	100	6	twice	twice	RB
cord-007211-prygoc0q	100	7	with	with	IN
cord-007211-prygoc0q	100	8	ice	ice	NN
cord-007211-prygoc0q	100	9	-	-	HYPH
cord-007211-prygoc0q	100	10	cold	cold	JJ
cord-007211-prygoc0q	100	11	PBS	PBS	NNP
cord-007211-prygoc0q	100	12	containing	contain	VBG
cord-007211-prygoc0q	100	13	20	20	CD
cord-007211-prygoc0q	100	14	mM	mm	CD
cord-007211-prygoc0q	100	15	IAA	IAA	NNP
cord-007211-prygoc0q	100	16	,	,	,
cord-007211-prygoc0q	100	17	lysed	lyse	VBN
cord-007211-prygoc0q	100	18	in	in	IN
cord-007211-prygoc0q	100	19	300/^1	300/^1	CD
cord-007211-prygoc0q	100	20	of	of	IN
cord-007211-prygoc0q	100	21	RIPA	RIPA	NNP
cord-007211-prygoc0q	100	22	buffer	buffer	NN
cord-007211-prygoc0q	100	23	containing	contain	VBG
cord-007211-prygoc0q	100	24	20	20	CD
cord-007211-prygoc0q	100	25	mM	mm	CD
cord-007211-prygoc0q	100	26	IAA	IAA	NNP
cord-007211-prygoc0q	100	27	,	,	,
cord-007211-prygoc0q	100	28	sonicated	sonicate	VBN
cord-007211-prygoc0q	100	29	for	for	IN
cord-007211-prygoc0q	100	30	30	30	CD
cord-007211-prygoc0q	100	31	s	s	NN
cord-007211-prygoc0q	100	32	,	,	,
cord-007211-prygoc0q	100	33	and	and	CC
cord-007211-prygoc0q	100	34	centrifuged	centrifuge	VBN
cord-007211-prygoc0q	100	35	for	for	IN
cord-007211-prygoc0q	100	36	10	10	CD
cord-007211-prygoc0q	100	37	min	min	NN
cord-007211-prygoc0q	100	38	at	at	IN
cord-007211-prygoc0q	100	39	15,000	15,000	CD
cord-007211-prygoc0q	100	40	rpm	rpm	NN
cord-007211-prygoc0q	100	41	.	.	.
cord-007211-prygoc0q	101	1	Fifty	fifty	CD
cord-007211-prygoc0q	101	2	microliters	microliter	NNS
cord-007211-prygoc0q	101	3	of	of	IN
cord-007211-prygoc0q	101	4	the	the	DT
cord-007211-prygoc0q	101	5	supernatant	supernatant	JJ
cord-007211-prygoc0q	101	6	(	(	-LRB-
cord-007211-prygoc0q	101	7	cellular	cellular	JJ
cord-007211-prygoc0q	101	8	extract	extract	NN
cord-007211-prygoc0q	101	9	)	)	-RRB-
cord-007211-prygoc0q	101	10	was	be	VBD
cord-007211-prygoc0q	101	11	incubated	incubate	VBN
cord-007211-prygoc0q	101	12	with	with	IN
cord-007211-prygoc0q	101	13	5	5	CD
cord-007211-prygoc0q	101	14	//I	//i	CD
cord-007211-prygoc0q	101	15	of	of	IN
cord-007211-prygoc0q	101	16	antibody	antibody	NN
cord-007211-prygoc0q	101	17	raised	raise	VBN
cord-007211-prygoc0q	101	18	against	against	IN
cord-007211-prygoc0q	101	19	recombinant	recombinant	JJ
cord-007211-prygoc0q	101	20	F	F	NNP
cord-007211-prygoc0q	101	21	protein	protein	NN
cord-007211-prygoc0q	101	22	expressed	express	VBN
cord-007211-prygoc0q	101	23	in	in	IN
cord-007211-prygoc0q	101	24	E.	E.	NNP
cord-007211-prygoc0q	101	25	coli	coli	NNS
cord-007211-prygoc0q	101	26	or	or	CC
cord-007211-prygoc0q	101	27	MAbs	MAbs	NNP
cord-007211-prygoc0q	101	28	reacting	react	VBG
cord-007211-prygoc0q	101	29	with	with	IN
cord-007211-prygoc0q	101	30	various	various	JJ
cord-007211-prygoc0q	101	31	epitopes	epitope	NNS
cord-007211-prygoc0q	101	32	(	(	-LRB-
cord-007211-prygoc0q	101	33	25	25	CD
cord-007211-prygoc0q	101	34	)	)	-RRB-
cord-007211-prygoc0q	101	35	on	on	IN
cord-007211-prygoc0q	101	36	ice	ice	NN
cord-007211-prygoc0q	101	37	for	for	IN
cord-007211-prygoc0q	101	38	1	1	CD
cord-007211-prygoc0q	101	39	h.	h.	NN
cord-007211-prygoc0q	101	40	Twenty	twenty	CD
cord-007211-prygoc0q	101	41	microliters	microliter	NNS
cord-007211-prygoc0q	101	42	of	of	IN
cord-007211-prygoc0q	101	43	a	a	DT
cord-007211-prygoc0q	101	44	suspension	suspension	NN
cord-007211-prygoc0q	101	45	of	of	IN
cord-007211-prygoc0q	101	46	protein	protein	NN
cord-007211-prygoc0q	101	47	-	-	:
cord-007211-prygoc0q	101	48	A	a	DT
cord-007211-prygoc0q	101	49	Sepharose	Sepharose	NNP
cord-007211-prygoc0q	101	50	Fast	Fast	NNP
cord-007211-prygoc0q	101	51	Flow	flow	NN
cord-007211-prygoc0q	102	1	(	(	-LRB-
cord-007211-prygoc0q	102	2	Pharmacia	Pharmacia	NNP
cord-007211-prygoc0q	102	3	Biotech	Biotech	NNP
cord-007211-prygoc0q	102	4	,	,	,
cord-007211-prygoc0q	102	5	Uppsala	Uppsala	NNP
cord-007211-prygoc0q	102	6	,	,	,
cord-007211-prygoc0q	102	7	Sweden	Sweden	NNP
cord-007211-prygoc0q	102	8	)	)	-RRB-
cord-007211-prygoc0q	102	9	was	be	VBD
cord-007211-prygoc0q	102	10	added	add	VBN
cord-007211-prygoc0q	102	11	to	to	IN
cord-007211-prygoc0q	102	12	the	the	DT
cord-007211-prygoc0q	102	13	lysates	lysate	NNS
cord-007211-prygoc0q	102	14	.	.	.
cord-007211-prygoc0q	103	1	The	the	DT
cord-007211-prygoc0q	103	2	mixture	mixture	NN
cord-007211-prygoc0q	103	3	was	be	VBD
cord-007211-prygoc0q	103	4	incubated	incubate	VBN
cord-007211-prygoc0q	103	5	at	at	IN
cord-007211-prygoc0q	103	6	4	4	CD
cord-007211-prygoc0q	103	7	°	°	NN
cord-007211-prygoc0q	103	8	C	c	NN
cord-007211-prygoc0q	103	9	for	for	IN
cord-007211-prygoc0q	103	10	1	1	CD
cord-007211-prygoc0q	103	11	h	h	NN
cord-007211-prygoc0q	103	12	with	with	IN
cord-007211-prygoc0q	103	13	gentle	gentle	JJ
cord-007211-prygoc0q	103	14	mixing	mixing	NN
cord-007211-prygoc0q	103	15	,	,	,
cord-007211-prygoc0q	103	16	and	and	CC
cord-007211-prygoc0q	103	17	immune	immune	JJ
cord-007211-prygoc0q	103	18	complexes	complex	NNS
cord-007211-prygoc0q	103	19	adsorbed	adsorb	VBN
cord-007211-prygoc0q	103	20	on	on	IN
cord-007211-prygoc0q	103	21	the	the	DT
cord-007211-prygoc0q	103	22	beads	bead	NNS
cord-007211-prygoc0q	103	23	were	be	VBD
cord-007211-prygoc0q	103	24	washed	wash	VBN
cord-007211-prygoc0q	103	25	three	three	CD
cord-007211-prygoc0q	103	26	times	time	NNS
cord-007211-prygoc0q	103	27	in	in	IN
cord-007211-prygoc0q	103	28	50	50	CD
cord-007211-prygoc0q	103	29	mM	mm	CD
cord-007211-prygoc0q	103	30	Tris	Tris	NNP
cord-007211-prygoc0q	103	31	-	-	HYPH
cord-007211-prygoc0q	103	32	HCl	HCl	NNP
cord-007211-prygoc0q	103	33	(	(	-LRB-
cord-007211-prygoc0q	103	34	pH	pH	NNP
cord-007211-prygoc0q	103	35	7.5	7.5	CD
cord-007211-prygoc0q	103	36	)	)	-RRB-
cord-007211-prygoc0q	103	37	,	,	,
cord-007211-prygoc0q	103	38	150	150	CD
cord-007211-prygoc0q	103	39	mM	mm	CD
cord-007211-prygoc0q	103	40	NaCl	nacl	NN
cord-007211-prygoc0q	103	41	,	,	,
cord-007211-prygoc0q	103	42	1	1	CD
cord-007211-prygoc0q	103	43	mM	mm	CD
cord-007211-prygoc0q	103	44	EDTA	EDTA	NNP
cord-007211-prygoc0q	103	45	,	,	,
cord-007211-prygoc0q	103	46	0.25	0.25	CD
cord-007211-prygoc0q	103	47	%	%	NN
cord-007211-prygoc0q	103	48	(	(	-LRB-
cord-007211-prygoc0q	103	49	w	w	NN
cord-007211-prygoc0q	103	50	/	/	SYM
cord-007211-prygoc0q	103	51	v	v	NN
cord-007211-prygoc0q	103	52	)	)	-RRB-
cord-007211-prygoc0q	103	53	gelatin	gelatin	NN
cord-007211-prygoc0q	103	54	,	,	,
cord-007211-prygoc0q	103	55	0.1	0.1	CD
cord-007211-prygoc0q	103	56	%	%	NN
cord-007211-prygoc0q	103	57	(	(	-LRB-
cord-007211-prygoc0q	103	58	v	v	NN
cord-007211-prygoc0q	103	59	/	/	SYM
cord-007211-prygoc0q	103	60	v	v	NN
cord-007211-prygoc0q	103	61	)	)	-RRB-
cord-007211-prygoc0q	103	62	NP-40	NP-40	NNP
cord-007211-prygoc0q	103	63	,	,	,
cord-007211-prygoc0q	103	64	0.02	0.02	CD
cord-007211-prygoc0q	103	65	%	%	NN
cord-007211-prygoc0q	103	66	(	(	-LRB-
cord-007211-prygoc0q	103	67	w	w	NN
cord-007211-prygoc0q	103	68	/	/	SYM
cord-007211-prygoc0q	103	69	v	v	NN
cord-007211-prygoc0q	103	70	)	)	-RRB-
cord-007211-prygoc0q	103	71	NaN	NaN	NNP
cord-007211-prygoc0q	103	72	3	3	CD
cord-007211-prygoc0q	103	73	,	,	,
cord-007211-prygoc0q	103	74	then	then	RB
cord-007211-prygoc0q	103	75	denatured	denature	VBN
cord-007211-prygoc0q	103	76	in	in	IN
cord-007211-prygoc0q	103	77	SDS	SDS	NNP
cord-007211-prygoc0q	103	78	sample	sample	NN
cord-007211-prygoc0q	103	79	buffer	buffer	NN
cord-007211-prygoc0q	103	80	[	[	-LRB-
cord-007211-prygoc0q	103	81	6.25	6.25	CD
cord-007211-prygoc0q	103	82	mM	mM	NNP
cord-007211-prygoc0q	103	83	Tris	Tris	NNP
cord-007211-prygoc0q	103	84	-	-	HYPH
cord-007211-prygoc0q	103	85	HCl	HCl	NNP
cord-007211-prygoc0q	103	86	(	(	-LRB-
cord-007211-prygoc0q	103	87	pH	pH	NNP
cord-007211-prygoc0q	103	88	6.8	6.8	CD
cord-007211-prygoc0q	103	89	)	)	-RRB-
cord-007211-prygoc0q	103	90	,	,	,
cord-007211-prygoc0q	103	91	2	2	CD
cord-007211-prygoc0q	103	92	%	%	NN
cord-007211-prygoc0q	103	93	SDS	SDS	NNP
cord-007211-prygoc0q	103	94	,	,	,
cord-007211-prygoc0q	103	95	10	10	CD
cord-007211-prygoc0q	103	96	%	%	NN
cord-007211-prygoc0q	103	97	glycerol	glycerol	NN
cord-007211-prygoc0q	103	98	]	]	-RRB-
cord-007211-prygoc0q	103	99	in	in	IN
cord-007211-prygoc0q	103	100	the	the	DT
cord-007211-prygoc0q	103	101	presence	presence	NN
cord-007211-prygoc0q	103	102	or	or	CC
cord-007211-prygoc0q	103	103	absence	absence	NN
cord-007211-prygoc0q	103	104	of	of	IN
cord-007211-prygoc0q	103	105	5	5	CD
cord-007211-prygoc0q	103	106	%	%	NN
cord-007211-prygoc0q	103	107	2-ME	2-me	CD
cord-007211-prygoc0q	103	108	by	by	IN
cord-007211-prygoc0q	103	109	boiling	boil	VBG
cord-007211-prygoc0q	103	110	for	for	IN
cord-007211-prygoc0q	103	111	3	3	CD
cord-007211-prygoc0q	103	112	min	min	NN
cord-007211-prygoc0q	103	113	.	.	.
cord-007211-prygoc0q	104	1	The	the	DT
cord-007211-prygoc0q	104	2	samples	sample	NNS
cord-007211-prygoc0q	104	3	were	be	VBD
cord-007211-prygoc0q	104	4	analyzed	analyze	VBN
cord-007211-prygoc0q	104	5	by	by	IN
cord-007211-prygoc0q	104	6	9	9	CD
cord-007211-prygoc0q	104	7	%	%	NN
cord-007211-prygoc0q	104	8	SDS	sds	NN
cord-007211-prygoc0q	104	9	-	-	HYPH
cord-007211-prygoc0q	104	10	PAGE	PAGE	NNP
cord-007211-prygoc0q	104	11	followed	follow	VBN
cord-007211-prygoc0q	104	12	by	by	IN
cord-007211-prygoc0q	104	13	fluorography	fluorography	NNP
cord-007211-prygoc0q	104	14	.	.	.
cord-007211-prygoc0q	105	1	Glycosidase	Glycosidase	NNP
cord-007211-prygoc0q	105	2	Treatment-	Treatment-	NNP
cord-007211-prygoc0q	106	1	The	the	DT
cord-007211-prygoc0q	106	2	immune	immune	JJ
cord-007211-prygoc0q	106	3	complexes	complex	NNS
cord-007211-prygoc0q	106	4	adsorbed	adsorb	VBN
cord-007211-prygoc0q	106	5	on	on	IN
cord-007211-prygoc0q	106	6	protein	protein	NN
cord-007211-prygoc0q	106	7	-	-	:
cord-007211-prygoc0q	106	8	A	A	NNP
cord-007211-prygoc0q	106	9	Sepharose	Sepharose	NNP
cord-007211-prygoc0q	106	10	were	be	VBD
cord-007211-prygoc0q	106	11	resuspended	resuspend	VBN
cord-007211-prygoc0q	106	12	in	in	IN
cord-007211-prygoc0q	106	13	100	100	CD
cord-007211-prygoc0q	106	14	fi\	fi\	NN
cord-007211-prygoc0q	106	15	of	of	IN
cord-007211-prygoc0q	106	16	50	50	CD
cord-007211-prygoc0q	106	17	mM	mm	CD
cord-007211-prygoc0q	106	18	sodium	sodium	NN
cord-007211-prygoc0q	106	19	acetate	acetate	NN
cord-007211-prygoc0q	106	20	buffer	buffer	NN
cord-007211-prygoc0q	106	21	(	(	-LRB-
cord-007211-prygoc0q	106	22	pH5.5	pH5.5	NNP
cord-007211-prygoc0q	106	23	)	)	-RRB-
cord-007211-prygoc0q	106	24	,	,	,
cord-007211-prygoc0q	106	25	then	then	RB
cord-007211-prygoc0q	106	26	samples	sample	NNS
cord-007211-prygoc0q	106	27	were	be	VBD
cord-007211-prygoc0q	106	28	incubated	incubate	VBN
cord-007211-prygoc0q	106	29	with	with	IN
cord-007211-prygoc0q	106	30	2	2	CD
cord-007211-prygoc0q	106	31	mU	mU	NNS
cord-007211-prygoc0q	106	32	of	of	IN
cord-007211-prygoc0q	106	33	endo	endo	NN
cord-007211-prygoc0q	106	34	-	-	HYPH
cord-007211-prygoc0q	106	35	y3-AT	y3-AT	NNP
cord-007211-prygoc0q	106	36	-	-	HYPH
cord-007211-prygoc0q	106	37	acetylglucosaminidase	acetylglucosaminidase	NN
cord-007211-prygoc0q	106	38	H	h	NN
cord-007211-prygoc0q	106	39	(	(	-LRB-
cord-007211-prygoc0q	106	40	endo	endo	NN
cord-007211-prygoc0q	106	41	H	h	NN
cord-007211-prygoc0q	106	42	:	:	:
cord-007211-prygoc0q	106	43	Boehringer	Boehringer	NNP
cord-007211-prygoc0q	106	44	Mannheim	Mannheim	NNP
cord-007211-prygoc0q	106	45	Biochemica	Biochemica	NNP
cord-007211-prygoc0q	106	46	)	)	-RRB-
cord-007211-prygoc0q	106	47	for	for	IN
cord-007211-prygoc0q	106	48	16	16	CD
cord-007211-prygoc0q	106	49	h	h	NN
cord-007211-prygoc0q	106	50	at	at	IN
cord-007211-prygoc0q	106	51	37	37	CD
cord-007211-prygoc0q	106	52	°	°	NN
cord-007211-prygoc0q	106	53	C	c	NN
cord-007211-prygoc0q	106	54	.	.	.
cord-007211-prygoc0q	107	1	After	after	IN
cord-007211-prygoc0q	107	2	digestion	digestion	NN
cord-007211-prygoc0q	107	3	,	,	,
cord-007211-prygoc0q	107	4	the	the	DT
cord-007211-prygoc0q	107	5	immune	immune	JJ
cord-007211-prygoc0q	107	6	complexes	complex	NNS
cord-007211-prygoc0q	107	7	on	on	IN
cord-007211-prygoc0q	107	8	the	the	DT
cord-007211-prygoc0q	107	9	beads	bead	NNS
cord-007211-prygoc0q	107	10	were	be	VBD
cord-007211-prygoc0q	107	11	recovered	recover	VBN
cord-007211-prygoc0q	107	12	by	by	IN
cord-007211-prygoc0q	107	13	centrifugation	centrifugation	NN
cord-007211-prygoc0q	107	14	and	and	CC
cord-007211-prygoc0q	107	15	denatured	denature	VBN
cord-007211-prygoc0q	107	16	in	in	IN
cord-007211-prygoc0q	107	17	SDS	SDS	NNP
cord-007211-prygoc0q	107	18	sample	sample	NN
cord-007211-prygoc0q	107	19	buffer	buffer	NN
cord-007211-prygoc0q	107	20	containing	contain	VBG
cord-007211-prygoc0q	107	21	5	5	CD
cord-007211-prygoc0q	107	22	%	%	NN
cord-007211-prygoc0q	107	23	2-ME	2-me	CD
cord-007211-prygoc0q	107	24	by	by	IN
cord-007211-prygoc0q	107	25	boiling	boil	VBG
cord-007211-prygoc0q	107	26	for	for	IN
cord-007211-prygoc0q	107	27	3	3	CD
cord-007211-prygoc0q	107	28	min	min	NN
cord-007211-prygoc0q	107	29	.	.	.
cord-007211-prygoc0q	108	1	The	the	DT
cord-007211-prygoc0q	108	2	immune	immune	JJ
cord-007211-prygoc0q	108	3	complexes	complex	NNS
cord-007211-prygoc0q	108	4	were	be	VBD
cord-007211-prygoc0q	108	5	analyzed	analyze	VBN
cord-007211-prygoc0q	108	6	by	by	IN
cord-007211-prygoc0q	108	7	SDS	SDS	NNP
cord-007211-prygoc0q	108	8	-	-	HYPH
cord-007211-prygoc0q	108	9	PAGE	PAGE	NNP
cord-007211-prygoc0q	108	10	followed	follow	VBN
cord-007211-prygoc0q	108	11	by	by	IN
cord-007211-prygoc0q	108	12	fluorography	fluorography	NNP
cord-007211-prygoc0q	108	13	.	.	.
cord-007211-prygoc0q	109	1	Expression	expression	NN
cord-007211-prygoc0q	109	2	of	of	IN
cord-007211-prygoc0q	109	3	the	the	DT
cord-007211-prygoc0q	109	4	Cysteine	Cysteine	NNP
cord-007211-prygoc0q	109	5	Mutant	Mutant	NNP
cord-007211-prygoc0q	109	6	F	F	NNP
cord-007211-prygoc0q	109	7	Proteins-	Proteins-	NNP
cord-007211-prygoc0q	110	1	The	the	DT
cord-007211-prygoc0q	110	2	F	F	NNP
cord-007211-prygoc0q	110	3	protein	protein	NN
cord-007211-prygoc0q	110	4	of	of	IN
cord-007211-prygoc0q	110	5	the	the	DT
cord-007211-prygoc0q	110	6	Z	z	NN
cord-007211-prygoc0q	110	7	strain	strain	NN
cord-007211-prygoc0q	110	8	of	of	IN
cord-007211-prygoc0q	110	9	Sendai	Sendai	NNP
cord-007211-prygoc0q	110	10	virus	virus	NN
cord-007211-prygoc0q	110	11	possesses	possess	VBZ
cord-007211-prygoc0q	110	12	12	12	CD
cord-007211-prygoc0q	110	13	cysteine	cysteine	NN
cord-007211-prygoc0q	110	14	residues	residue	NNS
cord-007211-prygoc0q	110	15	.	.	.
cord-007211-prygoc0q	111	1	The	the	DT
cord-007211-prygoc0q	111	2	first	first	JJ
cord-007211-prygoc0q	111	3	and	and	CC
cord-007211-prygoc0q	111	4	second	second	JJ
cord-007211-prygoc0q	111	5	cysteine	cysteine	NN
cord-007211-prygoc0q	111	6	residues	residue	NNS
cord-007211-prygoc0q	111	7	are	be	VBP
cord-007211-prygoc0q	111	8	present	present	JJ
cord-007211-prygoc0q	111	9	in	in	IN
cord-007211-prygoc0q	111	10	the	the	DT
cord-007211-prygoc0q	111	11	signal	signal	NN
cord-007211-prygoc0q	111	12	peptide	peptide	NN
cord-007211-prygoc0q	111	13	while	while	IN
cord-007211-prygoc0q	111	14	the	the	DT
cord-007211-prygoc0q	111	15	rest	rest	NN
cord-007211-prygoc0q	111	16	are	be	VBP
cord-007211-prygoc0q	111	17	distributed	distribute	VBN
cord-007211-prygoc0q	111	18	across	across	IN
cord-007211-prygoc0q	111	19	the	the	DT
cord-007211-prygoc0q	111	20	entire	entire	JJ
cord-007211-prygoc0q	111	21	ectodomain	ectodomain	NN
cord-007211-prygoc0q	111	22	of	of	IN
cord-007211-prygoc0q	111	23	the	the	DT
cord-007211-prygoc0q	111	24	protein	protein	NN
cord-007211-prygoc0q	111	25	.	.	.
cord-007211-prygoc0q	112	1	The	the	DT
cord-007211-prygoc0q	112	2	signal	signal	NN
cord-007211-prygoc0q	112	3	peptide	peptide	NN
cord-007211-prygoc0q	112	4	is	be	VBZ
cord-007211-prygoc0q	112	5	cleaved	cleave	VBN
cord-007211-prygoc0q	112	6	in	in	IN
cord-007211-prygoc0q	112	7	the	the	DT
cord-007211-prygoc0q	112	8	ER	er	NN
cord-007211-prygoc0q	112	9	,	,	,
cord-007211-prygoc0q	112	10	and	and	CC
cord-007211-prygoc0q	112	11	thus	thus	RB
cord-007211-prygoc0q	112	12	the	the	DT
cord-007211-prygoc0q	112	13	remaining	remain	VBG
cord-007211-prygoc0q	112	14	10	10	CD
cord-007211-prygoc0q	112	15	cysteine	cysteine	NN
cord-007211-prygoc0q	112	16	residues	residue	NNS
cord-007211-prygoc0q	112	17	are	be	VBP
cord-007211-prygoc0q	112	18	present	present	JJ
cord-007211-prygoc0q	112	19	in	in	IN
cord-007211-prygoc0q	112	20	the	the	DT
cord-007211-prygoc0q	112	21	mature	mature	JJ
cord-007211-prygoc0q	112	22	F	F	NNP
cord-007211-prygoc0q	112	23	protein	protein	NN
cord-007211-prygoc0q	112	24	.	.	.
cord-007211-prygoc0q	113	1	The	the	DT
cord-007211-prygoc0q	113	2	relative	relative	JJ
cord-007211-prygoc0q	113	3	locations	location	NNS
cord-007211-prygoc0q	113	4	of	of	IN
cord-007211-prygoc0q	113	5	these	these	DT
cord-007211-prygoc0q	113	6	cysteine	cysteine	NN
cord-007211-prygoc0q	113	7	residues	residue	NNS
cord-007211-prygoc0q	113	8	are	be	VBP
cord-007211-prygoc0q	113	9	highly	highly	RB
cord-007211-prygoc0q	113	10	conserved	conserve	VBN
cord-007211-prygoc0q	113	11	among	among	IN
cord-007211-prygoc0q	113	12	paramyxovirus	paramyxovirus	NNP
cord-007211-prygoc0q	113	13	F	F	NNP
cord-007211-prygoc0q	113	14	proteins	protein	NNS
cord-007211-prygoc0q	113	15	,	,	,
cord-007211-prygoc0q	113	16	and	and	CC
cord-007211-prygoc0q	113	17	these	these	DT
cord-007211-prygoc0q	113	18	cysteines	cysteine	NNS
cord-007211-prygoc0q	113	19	are	be	VBP
cord-007211-prygoc0q	113	20	likely	likely	JJ
cord-007211-prygoc0q	113	21	to	to	TO
cord-007211-prygoc0q	113	22	participate	participate	VB
cord-007211-prygoc0q	113	23	in	in	IN
cord-007211-prygoc0q	113	24	intramolecular	intramolecular	JJ
cord-007211-prygoc0q	113	25	disulfide	disulfide	JJ
cord-007211-prygoc0q	113	26	bonds	bond	NNS
cord-007211-prygoc0q	113	27	in	in	IN
cord-007211-prygoc0q	113	28	the	the	DT
cord-007211-prygoc0q	113	29	mature	mature	JJ
cord-007211-prygoc0q	113	30	protein	protein	NN
cord-007211-prygoc0q	113	31	.	.	.
cord-007211-prygoc0q	114	1	To	to	TO
cord-007211-prygoc0q	114	2	examine	examine	VB
cord-007211-prygoc0q	114	3	the	the	DT
cord-007211-prygoc0q	114	4	processing	processing	NN
cord-007211-prygoc0q	114	5	of	of	IN
cord-007211-prygoc0q	114	6	the	the	DT
cord-007211-prygoc0q	114	7	intramolecular	intramolecular	JJ
cord-007211-prygoc0q	114	8	disulfide	disulfide	JJ
cord-007211-prygoc0q	114	9	bonds	bond	NNS
cord-007211-prygoc0q	114	10	and	and	CC
cord-007211-prygoc0q	114	11	their	-PRON-	PRP$
cord-007211-prygoc0q	114	12	contribution	contribution	NN
cord-007211-prygoc0q	114	13	to	to	IN
cord-007211-prygoc0q	114	14	the	the	DT
cord-007211-prygoc0q	114	15	structure	structure	NN
cord-007211-prygoc0q	114	16	of	of	IN
cord-007211-prygoc0q	114	17	the	the	DT
cord-007211-prygoc0q	114	18	F	F	NNP
cord-007211-prygoc0q	114	19	protein	protein	NN
cord-007211-prygoc0q	114	20	,	,	,
cord-007211-prygoc0q	114	21	site	site	NN
cord-007211-prygoc0q	114	22	-	-	HYPH
cord-007211-prygoc0q	114	23	directed	direct	VBN
cord-007211-prygoc0q	114	24	mutagenesis	mutagenesis	NN
cord-007211-prygoc0q	114	25	using	use	VBG
cord-007211-prygoc0q	114	26	PCR	PCR	NNP
cord-007211-prygoc0q	114	27	was	be	VBD
cord-007211-prygoc0q	114	28	used	use	VBN
cord-007211-prygoc0q	114	29	to	to	TO
cord-007211-prygoc0q	114	30	substitute	substitute	VB
cord-007211-prygoc0q	114	31	each	each	DT
cord-007211-prygoc0q	114	32	cysteine	cysteine	NN
cord-007211-prygoc0q	114	33	residue	residue	NN
cord-007211-prygoc0q	114	34	by	by	IN
cord-007211-prygoc0q	114	35	a	a	DT
cord-007211-prygoc0q	114	36	serine	serine	NN
cord-007211-prygoc0q	114	37	residue	residue	NN
cord-007211-prygoc0q	114	38	to	to	TO
cord-007211-prygoc0q	114	39	prevent	prevent	VB
cord-007211-prygoc0q	114	40	the	the	DT
cord-007211-prygoc0q	114	41	formation	formation	NN
cord-007211-prygoc0q	114	42	of	of	IN
cord-007211-prygoc0q	114	43	disulfide	disulfide	JJ
cord-007211-prygoc0q	114	44	bonds	bond	NNS
cord-007211-prygoc0q	114	45	.	.	.
cord-007211-prygoc0q	115	1	Ten	ten	CD
cord-007211-prygoc0q	115	2	expression	expression	NN
cord-007211-prygoc0q	115	3	plasmids	plasmid	NNS
cord-007211-prygoc0q	115	4	of	of	IN
cord-007211-prygoc0q	115	5	the	the	DT
cord-007211-prygoc0q	115	6	cysteine	cysteine	NN
cord-007211-prygoc0q	115	7	mutant	mutant	NN
cord-007211-prygoc0q	115	8	F	F	NNP
cord-007211-prygoc0q	115	9	proteins	protein	NNS
cord-007211-prygoc0q	115	10	,	,	,
cord-007211-prygoc0q	115	11	named	name	VBN
cord-007211-prygoc0q	115	12	FC1S	fc1	NNS
cord-007211-prygoc0q	115	13	to	to	IN
cord-007211-prygoc0q	115	14	FC10S	fc10	NNS
cord-007211-prygoc0q	115	15	,	,	,
cord-007211-prygoc0q	115	16	were	be	VBD
cord-007211-prygoc0q	115	17	prepared	prepare	VBN
cord-007211-prygoc0q	115	18	as	as	IN
cord-007211-prygoc0q	115	19	shown	show	VBN
cord-007211-prygoc0q	115	20	in	in	IN
cord-007211-prygoc0q	115	21	Fig	Fig	NNP
cord-007211-prygoc0q	115	22	.	.	.
cord-007211-prygoc0q	116	1	1	1	LS
cord-007211-prygoc0q	116	2	.	.	.
cord-007211-prygoc0q	117	1	Recombinant	recombinant	JJ
cord-007211-prygoc0q	117	2	plasmids	plasmid	NNS
cord-007211-prygoc0q	117	3	were	be	VBD
cord-007211-prygoc0q	117	4	introduced	introduce	VBN
cord-007211-prygoc0q	117	5	into	into	IN
cord-007211-prygoc0q	117	6	COS	COS	NNP
cord-007211-prygoc0q	117	7	cells	cell	NNS
cord-007211-prygoc0q	117	8	by	by	IN
cord-007211-prygoc0q	117	9	the	the	DT
cord-007211-prygoc0q	117	10	calcium	calcium	NN
cord-007211-prygoc0q	117	11	phosphate	phosphate	NN
cord-007211-prygoc0q	117	12	precipitation	precipitation	NN
cord-007211-prygoc0q	117	13	method	method	NN
cord-007211-prygoc0q	117	14	(	(	-LRB-
cord-007211-prygoc0q	117	15	22	22	CD
cord-007211-prygoc0q	117	16	)	)	-RRB-
cord-007211-prygoc0q	117	17	,	,	,
cord-007211-prygoc0q	117	18	and	and	CC
cord-007211-prygoc0q	117	19	the	the	DT
cord-007211-prygoc0q	117	20	cells	cell	NNS
cord-007211-prygoc0q	117	21	were	be	VBD
cord-007211-prygoc0q	117	22	incubated	incubate	VBN
cord-007211-prygoc0q	117	23	for	for	IN
cord-007211-prygoc0q	117	24	48	48	CD
cord-007211-prygoc0q	117	25	h.	h.	NN
cord-007211-prygoc0q	117	26	Transfected	Transfected	NNP
cord-007211-prygoc0q	117	27	cells	cell	NNS
cord-007211-prygoc0q	117	28	were	be	VBD
cord-007211-prygoc0q	117	29	labeled	label	VBN
cord-007211-prygoc0q	117	30	with	with	IN
cord-007211-prygoc0q	117	31	[	[	-LRB-
cord-007211-prygoc0q	117	32	35	35	CD
cord-007211-prygoc0q	117	33	S]methionine	S]methionine	NNP
cord-007211-prygoc0q	117	34	and	and	CC
cord-007211-prygoc0q	117	35	[	[	-LRB-
cord-007211-prygoc0q	117	36	35	35	CD
cord-007211-prygoc0q	117	37	S	s	NN
cord-007211-prygoc0q	117	38	]	]	-RRB-
cord-007211-prygoc0q	117	39	cysteine	cysteine	NN
cord-007211-prygoc0q	117	40	for	for	IN
cord-007211-prygoc0q	117	41	10	10	CD
cord-007211-prygoc0q	117	42	min	min	NN
cord-007211-prygoc0q	117	43	,	,	,
cord-007211-prygoc0q	117	44	chased	chase	VBN
cord-007211-prygoc0q	117	45	for	for	IN
cord-007211-prygoc0q	117	46	1	1	CD
cord-007211-prygoc0q	117	47	h	h	NN
cord-007211-prygoc0q	117	48	,	,	,
cord-007211-prygoc0q	117	49	then	then	RB
cord-007211-prygoc0q	117	50	lysed	lyse	VBN
cord-007211-prygoc0q	117	51	in	in	IN
cord-007211-prygoc0q	117	52	RIPA	RIPA	NNP
cord-007211-prygoc0q	117	53	buffer	buffer	NN
cord-007211-prygoc0q	117	54	.	.	.
cord-007211-prygoc0q	118	1	The	the	DT
cord-007211-prygoc0q	118	2	lysates	lysate	NNS
cord-007211-prygoc0q	118	3	were	be	VBD
cord-007211-prygoc0q	118	4	immunoprecipitated	immunoprecipitate	VBN
cord-007211-prygoc0q	118	5	with	with	IN
cord-007211-prygoc0q	118	6	anti	anti	JJ
cord-007211-prygoc0q	118	7	-	-	JJ
cord-007211-prygoc0q	118	8	F	f	JJ
cord-007211-prygoc0q	118	9	antiserum	antiserum	NN
cord-007211-prygoc0q	118	10	and	and	CC
cord-007211-prygoc0q	118	11	analyzed	analyze	VBN
cord-007211-prygoc0q	118	12	by	by	IN
cord-007211-prygoc0q	118	13	9	9	CD
cord-007211-prygoc0q	118	14	%	%	NN
cord-007211-prygoc0q	118	15	SDS	sds	NN
cord-007211-prygoc0q	118	16	-	-	HYPH
cord-007211-prygoc0q	118	17	PAGE	page	NN
cord-007211-prygoc0q	118	18	under	under	IN
cord-007211-prygoc0q	118	19	reducing	reduce	VBG
cord-007211-prygoc0q	118	20	(	(	-LRB-
cord-007211-prygoc0q	118	21	Fig	Fig	NNP
cord-007211-prygoc0q	118	22	.	.	.
cord-007211-prygoc0q	118	23	2A	2a	CD
cord-007211-prygoc0q	118	24	)	)	-RRB-
cord-007211-prygoc0q	118	25	or	or	CC
cord-007211-prygoc0q	118	26	nonreducing	nonreducing	NN
cord-007211-prygoc0q	118	27	(	(	-LRB-
cord-007211-prygoc0q	118	28	Fig	Fig	NNP
cord-007211-prygoc0q	118	29	.	.	.
cord-007211-prygoc0q	118	30	2B	2b	LS
cord-007211-prygoc0q	118	31	)	)	-RRB-
cord-007211-prygoc0q	118	32	conditions	condition	NNS
cord-007211-prygoc0q	118	33	.	.	.
cord-007211-prygoc0q	119	1	In	in	IN
cord-007211-prygoc0q	119	2	Fig	Fig	NNP
cord-007211-prygoc0q	119	3	.	.	.
cord-007211-prygoc0q	120	1	2A	2A	NNP
cord-007211-prygoc0q	120	2	,	,	,
cord-007211-prygoc0q	121	1	pSRD	pSRD	NNP
cord-007211-prygoc0q	121	2	-	-	HYPH
cord-007211-prygoc0q	121	3	F	F	NNP
cord-007211-prygoc0q	121	4	transfected	transfecte	VBN
cord-007211-prygoc0q	121	5	cells	cell	NNS
cord-007211-prygoc0q	121	6	gave	give	VBD
cord-007211-prygoc0q	121	7	one	one	CD
cord-007211-prygoc0q	121	8	main	main	JJ
cord-007211-prygoc0q	121	9	band	band	NN
cord-007211-prygoc0q	121	10	(	(	-LRB-
cord-007211-prygoc0q	121	11	lane	lane	NN
cord-007211-prygoc0q	121	12	3	3	CD
cord-007211-prygoc0q	121	13	)	)	-RRB-
cord-007211-prygoc0q	121	14	with	with	IN
cord-007211-prygoc0q	121	15	mobility	mobility	NN
cord-007211-prygoc0q	121	16	corresponding	correspond	VBG
cord-007211-prygoc0q	121	17	to	to	IN
cord-007211-prygoc0q	121	18	the	the	DT
cord-007211-prygoc0q	121	19	uncleaved	uncleaved	JJ
cord-007211-prygoc0q	121	20	form	form	NN
cord-007211-prygoc0q	121	21	of	of	IN
cord-007211-prygoc0q	121	22	F	F	NNP
cord-007211-prygoc0q	121	23	,	,	,
cord-007211-prygoc0q	121	24	or	or	CC
cord-007211-prygoc0q	121	25	F	F	NNP
cord-007211-prygoc0q	121	26	o	o	NNP
cord-007211-prygoc0q	121	27	,	,	,
cord-007211-prygoc0q	121	28	synthesized	synthesize	VBN
cord-007211-prygoc0q	121	29	in	in	IN
cord-007211-prygoc0q	121	30	Sendai	Sendai	NNP
cord-007211-prygoc0q	121	31	virusinfected	virusinfecte	VBN
cord-007211-prygoc0q	121	32	COS	COS	NNP
cord-007211-prygoc0q	121	33	cells	cell	NNS
cord-007211-prygoc0q	121	34	as	as	IN
cord-007211-prygoc0q	121	35	reported	report	VBD
cord-007211-prygoc0q	121	36	previously	previously	RB
cord-007211-prygoc0q	121	37	(	(	-LRB-
cord-007211-prygoc0q	121	38	16	16	CD
cord-007211-prygoc0q	121	39	)	)	-RRB-
cord-007211-prygoc0q	121	40	.	.	.
cord-007211-prygoc0q	122	1	The	the	DT
cord-007211-prygoc0q	122	2	10	10	CD
cord-007211-prygoc0q	122	3	cysteine	cysteine	NN
cord-007211-prygoc0q	122	4	mutant	mutant	NN
cord-007211-prygoc0q	122	5	-	-	HYPH
cord-007211-prygoc0q	122	6	transfected	transfecte	VBN
cord-007211-prygoc0q	122	7	cells	cell	NNS
cord-007211-prygoc0q	122	8	also	also	RB
cord-007211-prygoc0q	122	9	gave	give	VBD
cord-007211-prygoc0q	122	10	one	one	CD
cord-007211-prygoc0q	122	11	main	main	JJ
cord-007211-prygoc0q	122	12	band	band	NN
cord-007211-prygoc0q	122	13	corresponding	correspond	VBG
cord-007211-prygoc0q	122	14	to	to	IN
cord-007211-prygoc0q	122	15	the	the	DT
cord-007211-prygoc0q	122	16	uncleaved	uncleaved	JJ
cord-007211-prygoc0q	122	17	F	F	NNP
cord-007211-prygoc0q	122	18	o	o	NNP
cord-007211-prygoc0q	122	19	protein	protein	NN
cord-007211-prygoc0q	122	20	.	.	.
cord-007211-prygoc0q	123	1	A	a	DT
cord-007211-prygoc0q	123	2	minor	minor	JJ
cord-007211-prygoc0q	123	3	band	band	NN
cord-007211-prygoc0q	123	4	of	of	IN
cord-007211-prygoc0q	123	5	about	about	RB
cord-007211-prygoc0q	123	6	60	60	CD
cord-007211-prygoc0q	123	7	kDa	kDa	NNS
cord-007211-prygoc0q	123	8	was	be	VBD
cord-007211-prygoc0q	123	9	also	also	RB
cord-007211-prygoc0q	123	10	detected	detect	VBN
cord-007211-prygoc0q	123	11	in	in	IN
cord-007211-prygoc0q	123	12	all	all	DT
cord-007211-prygoc0q	123	13	cells	cell	NNS
cord-007211-prygoc0q	123	14	transfected	transfecte	VBN
cord-007211-prygoc0q	123	15	with	with	IN
cord-007211-prygoc0q	123	16	recombinant	recombinant	JJ
cord-007211-prygoc0q	123	17	plasmids	plasmid	NNS
cord-007211-prygoc0q	123	18	(	(	-LRB-
cord-007211-prygoc0q	123	19	lanes	lane	NNS
cord-007211-prygoc0q	123	20	3	3	CD
cord-007211-prygoc0q	123	21	-	-	SYM
cord-007211-prygoc0q	123	22	13	13	CD
cord-007211-prygoc0q	123	23	)	)	-RRB-
cord-007211-prygoc0q	123	24	,	,	,
cord-007211-prygoc0q	123	25	and	and	CC
cord-007211-prygoc0q	123	26	this	this	DT
cord-007211-prygoc0q	123	27	seems	seem	VBZ
cord-007211-prygoc0q	123	28	to	to	TO
cord-007211-prygoc0q	123	29	be	be	VB
cord-007211-prygoc0q	123	30	nonspecifically	nonspecifically	RB
cord-007211-prygoc0q	123	31	degraded	degrade	VBN
cord-007211-prygoc0q	123	32	F	F	NNP
cord-007211-prygoc0q	123	33	proteins	protein	NNS
cord-007211-prygoc0q	123	34	or	or	CC
cord-007211-prygoc0q	123	35	unglycosylated	unglycosylate	VBD
cord-007211-prygoc0q	123	36	F	F	NNP
cord-007211-prygoc0q	123	37	proteins	protein	NNS
cord-007211-prygoc0q	123	38	,	,	,
cord-007211-prygoc0q	123	39	because	because	IN
cord-007211-prygoc0q	123	40	the	the	DT
cord-007211-prygoc0q	123	41	unglycosylated	unglycosylated	JJ
cord-007211-prygoc0q	123	42	F	F	NNP
cord-007211-prygoc0q	123	43	proteins	protein	NNS
cord-007211-prygoc0q	123	44	and	and	CC
cord-007211-prygoc0q	123	45	immature	immature	JJ
cord-007211-prygoc0q	123	46	F	F	NNP
cord-007211-prygoc0q	123	47	proteins	protein	NNS
cord-007211-prygoc0q	123	48	could	could	MD
cord-007211-prygoc0q	123	49	be	be	VB
cord-007211-prygoc0q	123	50	detected	detect	VBN
cord-007211-prygoc0q	123	51	by	by	IN
cord-007211-prygoc0q	123	52	the	the	DT
cord-007211-prygoc0q	123	53	antiserum	antiserum	NN
cord-007211-prygoc0q	123	54	used	use	VBN
cord-007211-prygoc0q	123	55	here	here	RB
cord-007211-prygoc0q	123	56	,	,	,
cord-007211-prygoc0q	123	57	which	which	WDT
cord-007211-prygoc0q	123	58	was	be	VBD
cord-007211-prygoc0q	123	59	raised	raise	VBN
cord-007211-prygoc0q	123	60	against	against	IN
cord-007211-prygoc0q	123	61	the	the	DT
cord-007211-prygoc0q	123	62	unglycosylated	unglycosylated	JJ
cord-007211-prygoc0q	123	63	F	F	NNP
cord-007211-prygoc0q	123	64	proteins	protein	NNS
cord-007211-prygoc0q	123	65	expressed	express	VBN
cord-007211-prygoc0q	123	66	in	in	IN
cord-007211-prygoc0q	123	67	E.	E.	NNP
cord-007211-prygoc0q	123	68	coli	coli	NNS
cord-007211-prygoc0q	123	69	.	.	.
cord-007211-prygoc0q	124	1	The	the	DT
cord-007211-prygoc0q	124	2	cysteine	cysteine	NN
cord-007211-prygoc0q	124	3	mutant	mutant	NN
cord-007211-prygoc0q	125	1	pSRD	psrd	JJ
cord-007211-prygoc0q	125	2	-	-	HYPH
cord-007211-prygoc0q	125	3	FC1S	fc1	NNS
cord-007211-prygoc0q	126	1	(	(	-LRB-
cord-007211-prygoc0q	126	2	Fig	Fig	NNP
cord-007211-prygoc0q	126	3	.	.	.
cord-007211-prygoc0q	126	4	2A	2a	CD
cord-007211-prygoc0q	126	5	,	,	,
cord-007211-prygoc0q	126	6	lane	lane	NN
cord-007211-prygoc0q	126	7	4	4	CD
cord-007211-prygoc0q	126	8	)	)	-RRB-
cord-007211-prygoc0q	127	1	transfected	transfecte	VBN
cord-007211-prygoc0q	127	2	cells	cell	NNS
cord-007211-prygoc0q	127	3	gave	give	VBD
cord-007211-prygoc0q	127	4	three	three	CD
cord-007211-prygoc0q	127	5	bands	band	NNS
cord-007211-prygoc0q	127	6	,	,	,
cord-007211-prygoc0q	127	7	one	one	CD
cord-007211-prygoc0q	127	8	main	main	JJ
cord-007211-prygoc0q	127	9	band	band	NN
cord-007211-prygoc0q	127	10	corresponding	correspond	VBG
cord-007211-prygoc0q	127	11	to	to	IN
cord-007211-prygoc0q	127	12	the	the	DT
cord-007211-prygoc0q	127	13	F	F	NNP
cord-007211-prygoc0q	127	14	o	o	NN
cord-007211-prygoc0q	127	15	protein	protein	NN
cord-007211-prygoc0q	127	16	,	,	,
cord-007211-prygoc0q	127	17	the	the	DT
cord-007211-prygoc0q	127	18	common	common	JJ
cord-007211-prygoc0q	127	19	minor	minor	JJ
cord-007211-prygoc0q	127	20	band	band	NN
cord-007211-prygoc0q	127	21	(	(	-LRB-
cord-007211-prygoc0q	127	22	60	60	CD
cord-007211-prygoc0q	127	23	kDa	kDa	NNPS
cord-007211-prygoc0q	127	24	)	)	-RRB-
cord-007211-prygoc0q	127	25	,	,	,
cord-007211-prygoc0q	127	26	and	and	CC
cord-007211-prygoc0q	127	27	another	another	DT
cord-007211-prygoc0q	127	28	higher	high	JJR
cord-007211-prygoc0q	127	29	molecular	molecular	JJ
cord-007211-prygoc0q	127	30	weight	weight	NN
cord-007211-prygoc0q	127	31	species	species	NN
cord-007211-prygoc0q	127	32	of	of	IN
cord-007211-prygoc0q	127	33	F	F	NNP
cord-007211-prygoc0q	127	34	protein	protein	NN
cord-007211-prygoc0q	127	35	.	.	.
cord-007211-prygoc0q	128	1	The	the	DT
cord-007211-prygoc0q	128	2	alteration	alteration	NN
cord-007211-prygoc0q	128	3	of	of	IN
cord-007211-prygoc0q	128	4	the	the	DT
cord-007211-prygoc0q	128	5	cysteine	cysteine	NN
cord-007211-prygoc0q	128	6	residue	residue	NN
cord-007211-prygoc0q	128	7	of	of	IN
cord-007211-prygoc0q	128	8	the	the	DT
cord-007211-prygoc0q	128	9	F	F	NNP
cord-007211-prygoc0q	128	10	2	2	CD
cord-007211-prygoc0q	128	11	subunit	subunit	NN
cord-007211-prygoc0q	128	12	to	to	IN
cord-007211-prygoc0q	128	13	a	a	DT
cord-007211-prygoc0q	128	14	serine	serine	NN
cord-007211-prygoc0q	128	15	residue	residue	NN
cord-007211-prygoc0q	128	16	introduced	introduce	VBD
cord-007211-prygoc0q	128	17	a	a	DT
cord-007211-prygoc0q	128	18	new	new	JJ
cord-007211-prygoc0q	128	19	glycosylation	glycosylation	NN
cord-007211-prygoc0q	128	20	site	site	NN
cord-007211-prygoc0q	128	21	in	in	IN
cord-007211-prygoc0q	128	22	this	this	DT
cord-007211-prygoc0q	128	23	protein	protein	NN
cord-007211-prygoc0q	128	24	sequence	sequence	NN
cord-007211-prygoc0q	128	25	.	.	.
cord-007211-prygoc0q	129	1	Thus	thus	RB
cord-007211-prygoc0q	129	2	,	,	,
cord-007211-prygoc0q	129	3	the	the	DT
cord-007211-prygoc0q	129	4	higher	high	JJR
cord-007211-prygoc0q	129	5	molecular	molecular	JJ
cord-007211-prygoc0q	129	6	weight	weight	NN
cord-007211-prygoc0q	129	7	species	specie	NNS
cord-007211-prygoc0q	129	8	seems	seem	VBZ
cord-007211-prygoc0q	129	9	to	to	TO
cord-007211-prygoc0q	129	10	be	be	VB
cord-007211-prygoc0q	129	11	the	the	DT
cord-007211-prygoc0q	129	12	F	F	NNP
cord-007211-prygoc0q	129	13	protein	protein	NN
cord-007211-prygoc0q	129	14	utilizing	utilize	VBG
cord-007211-prygoc0q	129	15	the	the	DT
cord-007211-prygoc0q	129	16	additional	additional	JJ
cord-007211-prygoc0q	129	17	new	new	JJ
cord-007211-prygoc0q	129	18	glycosylation	glycosylation	NN
cord-007211-prygoc0q	129	19	site	site	NN
cord-007211-prygoc0q	129	20	.	.	.
cord-007211-prygoc0q	130	1	Approximately	approximately	RB
cord-007211-prygoc0q	130	2	equal	equal	JJ
cord-007211-prygoc0q	130	3	levels	level	NNS
cord-007211-prygoc0q	130	4	of	of	IN
cord-007211-prygoc0q	130	5	most	most	JJS
cord-007211-prygoc0q	130	6	of	of	IN
cord-007211-prygoc0q	130	7	the	the	DT
cord-007211-prygoc0q	130	8	F	F	NNP
cord-007211-prygoc0q	130	9	proteins	protein	NNS
cord-007211-prygoc0q	130	10	in	in	IN
cord-007211-prygoc0q	130	11	the	the	DT
cord-007211-prygoc0q	130	12	cells	cell	NNS
cord-007211-prygoc0q	130	13	transfected	transfecte	VBN
cord-007211-prygoc0q	130	14	with	with	IN
cord-007211-prygoc0q	130	15	the	the	DT
cord-007211-prygoc0q	130	16	above	above	JJ
cord-007211-prygoc0q	130	17	plasmids	plasmid	NNS
cord-007211-prygoc0q	130	18	were	be	VBD
cord-007211-prygoc0q	130	19	detected	detect	VBN
cord-007211-prygoc0q	130	20	by	by	IN
cord-007211-prygoc0q	130	21	Western	western	JJ
cord-007211-prygoc0q	130	22	blot	blot	NN
cord-007211-prygoc0q	130	23	analysis	analysis	NN
cord-007211-prygoc0q	130	24	(	(	-LRB-
cord-007211-prygoc0q	130	25	data	datum	NNS
cord-007211-prygoc0q	130	26	not	not	RB
cord-007211-prygoc0q	130	27	shown	show	VBN
cord-007211-prygoc0q	130	28	)	)	-RRB-
cord-007211-prygoc0q	130	29	.	.	.
cord-007211-prygoc0q	131	1	This	this	DT
cord-007211-prygoc0q	131	2	showed	show	VBD
cord-007211-prygoc0q	131	3	that	that	IN
cord-007211-prygoc0q	131	4	these	these	DT
cord-007211-prygoc0q	131	5	10	10	CD
cord-007211-prygoc0q	131	6	cysteine	cysteine	NN
cord-007211-prygoc0q	131	7	mutant	mutant	NN
cord-007211-prygoc0q	131	8	F	F	NNP
cord-007211-prygoc0q	131	9	proteins	protein	NNS
cord-007211-prygoc0q	131	10	were	be	VBD
cord-007211-prygoc0q	131	11	efficiently	efficiently	RB
cord-007211-prygoc0q	131	12	expressed	express	VBN
cord-007211-prygoc0q	131	13	at	at	IN
cord-007211-prygoc0q	131	14	almost	almost	RB
cord-007211-prygoc0q	131	15	the	the	DT
cord-007211-prygoc0q	131	16	same	same	JJ
cord-007211-prygoc0q	131	17	level	level	NN
cord-007211-prygoc0q	131	18	as	as	IN
cord-007211-prygoc0q	131	19	wild	wild	JJ
cord-007211-prygoc0q	131	20	-	-	HYPH
cord-007211-prygoc0q	131	21	type	type	NN
cord-007211-prygoc0q	131	22	F	F	NNP
cord-007211-prygoc0q	131	23	protein	protein	NN
cord-007211-prygoc0q	131	24	in	in	IN
cord-007211-prygoc0q	131	25	pSRD	pSRD	NNP
cord-007211-prygoc0q	131	26	-	-	HYPH
cord-007211-prygoc0q	131	27	F	f	NN
cord-007211-prygoc0q	131	28	transfected	transfecte	VBD
cord-007211-prygoc0q	131	29	cells	cell	NNS
cord-007211-prygoc0q	131	30	and	and	CC
cord-007211-prygoc0q	131	31	were	be	VBD
cord-007211-prygoc0q	131	32	relatively	relatively	RB
cord-007211-prygoc0q	131	33	stable	stable	JJ
cord-007211-prygoc0q	131	34	.	.	.
cord-007211-prygoc0q	132	1	Under	under	IN
cord-007211-prygoc0q	132	2	nonreducing	nonreducing	NN
cord-007211-prygoc0q	132	3	conditions	condition	NNS
cord-007211-prygoc0q	132	4	,	,	,
cord-007211-prygoc0q	132	5	formation	formation	NN
cord-007211-prygoc0q	132	6	of	of	IN
cord-007211-prygoc0q	132	7	disulfide	disulfide	JJ
cord-007211-prygoc0q	132	8	bonds	bond	NNS
cord-007211-prygoc0q	132	9	in	in	IN
cord-007211-prygoc0q	132	10	a	a	DT
cord-007211-prygoc0q	132	11	protein	protein	NN
cord-007211-prygoc0q	132	12	should	should	MD
cord-007211-prygoc0q	132	13	allow	allow	VB
cord-007211-prygoc0q	132	14	it	-PRON-	PRP
cord-007211-prygoc0q	132	15	to	to	TO
cord-007211-prygoc0q	132	16	assume	assume	VB
cord-007211-prygoc0q	132	17	a	a	DT
cord-007211-prygoc0q	132	18	more	more	RBR
cord-007211-prygoc0q	132	19	compact	compact	JJ
cord-007211-prygoc0q	132	20	form	form	NN
cord-007211-prygoc0q	132	21	and	and	CC
cord-007211-prygoc0q	132	22	to	to	TO
cord-007211-prygoc0q	132	23	migrate	migrate	VB
cord-007211-prygoc0q	132	24	faster	fast	RBR
cord-007211-prygoc0q	132	25	on	on	IN
cord-007211-prygoc0q	132	26	SDS	SDS	NNP
cord-007211-prygoc0q	132	27	-	-	HYPH
cord-007211-prygoc0q	132	28	PAGE	PAGE	NNP
cord-007211-prygoc0q	132	29	than	than	IN
cord-007211-prygoc0q	132	30	unfolded	unfolded	JJ
cord-007211-prygoc0q	132	31	forms	form	NNS
cord-007211-prygoc0q	132	32	without	without	IN
cord-007211-prygoc0q	132	33	disulfide	disulfide	JJ
cord-007211-prygoc0q	132	34	bonds	bond	NNS
cord-007211-prygoc0q	132	35	,	,	,
cord-007211-prygoc0q	132	36	as	as	IN
cord-007211-prygoc0q	132	37	reported	report	VBN
cord-007211-prygoc0q	132	38	by	by	IN
cord-007211-prygoc0q	132	39	Machamer	Machamer	NNP
cord-007211-prygoc0q	132	40	et	et	NNP
cord-007211-prygoc0q	132	41	al	al	NNP
cord-007211-prygoc0q	132	42	.	.	.
cord-007211-prygoc0q	133	1	(	(	-LRB-
cord-007211-prygoc0q	133	2	27	27	CD
cord-007211-prygoc0q	133	3	)	)	-RRB-
cord-007211-prygoc0q	133	4	.	.	.
cord-007211-prygoc0q	134	1	As	as	IN
cord-007211-prygoc0q	134	2	shown	show	VBN
cord-007211-prygoc0q	134	3	in	in	IN
cord-007211-prygoc0q	134	4	Fig	Fig	NNP
cord-007211-prygoc0q	134	5	.	.	.
cord-007211-prygoc0q	134	6	2B	2b	CD
cord-007211-prygoc0q	134	7	,	,	,
cord-007211-prygoc0q	134	8	the	the	DT
cord-007211-prygoc0q	134	9	compact	compact	JJ
cord-007211-prygoc0q	134	10	and	and	CC
cord-007211-prygoc0q	134	11	unfolded	unfolded	JJ
cord-007211-prygoc0q	134	12	forms	form	NNS
cord-007211-prygoc0q	134	13	of	of	IN
cord-007211-prygoc0q	134	14	monomer	monomer	NN
cord-007211-prygoc0q	134	15	F	F	NNP
cord-007211-prygoc0q	134	16	proteins	protein	NNS
cord-007211-prygoc0q	134	17	and	and	CC
cord-007211-prygoc0q	134	18	aggregates	aggregate	NNS
cord-007211-prygoc0q	134	19	were	be	VBD
cord-007211-prygoc0q	134	20	detected	detect	VBN
cord-007211-prygoc0q	134	21	with	with	IN
cord-007211-prygoc0q	134	22	the	the	DT
cord-007211-prygoc0q	134	23	mutants	mutant	NNS
cord-007211-prygoc0q	134	24	as	as	RB
cord-007211-prygoc0q	134	25	well	well	RB
cord-007211-prygoc0q	134	26	as	as	IN
cord-007211-prygoc0q	134	27	the	the	DT
cord-007211-prygoc0q	134	28	wild	wild	JJ
cord-007211-prygoc0q	134	29	-	-	HYPH
cord-007211-prygoc0q	134	30	type	type	NN
cord-007211-prygoc0q	134	31	F	F	NNP
cord-007211-prygoc0q	134	32	protein	protein	NN
cord-007211-prygoc0q	134	33	.	.	.
cord-007211-prygoc0q	135	1	The	the	DT
cord-007211-prygoc0q	135	2	compact	compact	JJ
cord-007211-prygoc0q	135	3	form	form	NN
cord-007211-prygoc0q	135	4	migrated	migrate	VBD
cord-007211-prygoc0q	135	5	as	as	IN
cord-007211-prygoc0q	135	6	a	a	DT
cord-007211-prygoc0q	135	7	60	60	CD
cord-007211-prygoc0q	135	8	kDa	kDa	NNP
cord-007211-prygoc0q	135	9	band	band	NN
cord-007211-prygoc0q	135	10	and	and	CC
cord-007211-prygoc0q	135	11	the	the	DT
cord-007211-prygoc0q	135	12	unfolded	unfold	VBN
cord-007211-prygoc0q	135	13	form	form	NN
cord-007211-prygoc0q	135	14	migrated	migrate	VBD
cord-007211-prygoc0q	135	15	as	as	IN
cord-007211-prygoc0q	135	16	a	a	DT
cord-007211-prygoc0q	135	17	66	66	CD
cord-007211-prygoc0q	135	18	kDa	kDa	NNP
cord-007211-prygoc0q	135	19	band	band	NN
cord-007211-prygoc0q	135	20	,	,	,
cord-007211-prygoc0q	135	21	which	which	WDT
cord-007211-prygoc0q	135	22	is	be	VBZ
cord-007211-prygoc0q	135	23	similar	similar	JJ
cord-007211-prygoc0q	135	24	to	to	IN
cord-007211-prygoc0q	135	25	the	the	DT
cord-007211-prygoc0q	135	26	molecular	molecular	JJ
cord-007211-prygoc0q	135	27	mass	mass	NN
cord-007211-prygoc0q	135	28	of	of	IN
cord-007211-prygoc0q	135	29	the	the	DT
cord-007211-prygoc0q	135	30	reduced	reduce	VBN
cord-007211-prygoc0q	135	31	form	form	NN
cord-007211-prygoc0q	135	32	of	of	IN
cord-007211-prygoc0q	135	33	F	F	NNP
cord-007211-prygoc0q	135	34	protein	protein	NN
cord-007211-prygoc0q	135	35	as	as	IN
cord-007211-prygoc0q	135	36	shown	show	VBN
cord-007211-prygoc0q	135	37	in	in	IN
cord-007211-prygoc0q	135	38	Fig	Fig	NNP
cord-007211-prygoc0q	135	39	.	.	.
cord-007211-prygoc0q	136	1	2A	2A	NNP
cord-007211-prygoc0q	136	2	.	.	.
cord-007211-prygoc0q	137	1	Most	Most	JJS
cord-007211-prygoc0q	137	2	of	of	IN
cord-007211-prygoc0q	137	3	the	the	DT
cord-007211-prygoc0q	137	4	wild	wild	JJ
cord-007211-prygoc0q	137	5	-	-	HYPH
cord-007211-prygoc0q	137	6	type	type	NN
cord-007211-prygoc0q	137	7	F	F	NNP
cord-007211-prygoc0q	137	8	protein	protein	NN
cord-007211-prygoc0q	137	9	migrated	migrate	VBD
cord-007211-prygoc0q	137	10	as	as	IN
cord-007211-prygoc0q	137	11	the	the	DT
cord-007211-prygoc0q	137	12	compact	compact	JJ
cord-007211-prygoc0q	137	13	form	form	NN
cord-007211-prygoc0q	137	14	(	(	-LRB-
cord-007211-prygoc0q	137	15	lane	lane	NN
cord-007211-prygoc0q	137	16	3	3	CD
cord-007211-prygoc0q	137	17	)	)	-RRB-
cord-007211-prygoc0q	137	18	.	.	.
cord-007211-prygoc0q	138	1	On	on	IN
cord-007211-prygoc0q	138	2	the	the	DT
cord-007211-prygoc0q	138	3	other	other	JJ
cord-007211-prygoc0q	138	4	hand	hand	NN
cord-007211-prygoc0q	138	5	,	,	,
cord-007211-prygoc0q	138	6	the	the	DT
cord-007211-prygoc0q	138	7	relative	relative	JJ
cord-007211-prygoc0q	138	8	amount	amount	NN
cord-007211-prygoc0q	138	9	of	of	IN
cord-007211-prygoc0q	138	10	the	the	DT
cord-007211-prygoc0q	138	11	unfolded	unfold	VBN
cord-007211-prygoc0q	138	12	form	form	NN
cord-007211-prygoc0q	138	13	of	of	IN
cord-007211-prygoc0q	138	14	the	the	DT
cord-007211-prygoc0q	138	15	each	each	DT
cord-007211-prygoc0q	138	16	10	10	CD
cord-007211-prygoc0q	138	17	cysteine	cysteine	NN
cord-007211-prygoc0q	138	18	mutant	mutant	NN
cord-007211-prygoc0q	138	19	F	F	NNP
cord-007211-prygoc0q	138	20	proteins	protein	NNS
cord-007211-prygoc0q	138	21	was	be	VBD
cord-007211-prygoc0q	138	22	increased	increase	VBN
cord-007211-prygoc0q	138	23	,	,	,
cord-007211-prygoc0q	138	24	and	and	CC
cord-007211-prygoc0q	138	25	the	the	DT
cord-007211-prygoc0q	138	26	ratio	ratio	NN
cord-007211-prygoc0q	138	27	of	of	IN
cord-007211-prygoc0q	138	28	the	the	DT
cord-007211-prygoc0q	138	29	compact	compact	JJ
cord-007211-prygoc0q	138	30	form	form	NN
cord-007211-prygoc0q	138	31	to	to	IN
cord-007211-prygoc0q	138	32	unfolded	unfold	VBN
cord-007211-prygoc0q	138	33	form	form	NN
cord-007211-prygoc0q	138	34	was	be	VBD
cord-007211-prygoc0q	138	35	roughly	roughly	RB
cord-007211-prygoc0q	138	36	1	1	CD
cord-007211-prygoc0q	138	37	as	as	IN
cord-007211-prygoc0q	138	38	determined	determine	VBN
cord-007211-prygoc0q	138	39	by	by	IN
cord-007211-prygoc0q	138	40	densitometry	densitometry	NNP
cord-007211-prygoc0q	138	41	(	(	-LRB-
cord-007211-prygoc0q	138	42	lanes	lane	NNS
cord-007211-prygoc0q	138	43	[	[	-LRB-
cord-007211-prygoc0q	138	44	4	4	CD
cord-007211-prygoc0q	138	45	]	]	-RRB-
cord-007211-prygoc0q	139	1	[	[	-LRB-
cord-007211-prygoc0q	139	2	5	5	CD
cord-007211-prygoc0q	139	3	]	]	-RRB-
cord-007211-prygoc0q	139	4	[	[	-LRB-
cord-007211-prygoc0q	139	5	6	6	CD
cord-007211-prygoc0q	139	6	]	]	-RRB-
cord-007211-prygoc0q	139	7	[	[	-LRB-
cord-007211-prygoc0q	139	8	7	7	CD
cord-007211-prygoc0q	139	9	]	]	-RRB-
cord-007211-prygoc0q	140	1	[	[	-LRB-
cord-007211-prygoc0q	140	2	8	8	CD
cord-007211-prygoc0q	140	3	]	]	-RRB-
cord-007211-prygoc0q	140	4	[	[	-LRB-
cord-007211-prygoc0q	140	5	9	9	CD
cord-007211-prygoc0q	140	6	]	]	-RRB-
cord-007211-prygoc0q	140	7	[	[	-LRB-
cord-007211-prygoc0q	140	8	10	10	CD
cord-007211-prygoc0q	140	9	]	]	-RRB-
cord-007211-prygoc0q	140	10	[	[	-LRB-
cord-007211-prygoc0q	140	11	11	11	CD
cord-007211-prygoc0q	140	12	]	]	-RRB-
cord-007211-prygoc0q	141	1	[	[	-LRB-
cord-007211-prygoc0q	141	2	12	12	CD
cord-007211-prygoc0q	141	3	]	]	-RRB-
cord-007211-prygoc0q	141	4	[	[	-LRB-
cord-007211-prygoc0q	141	5	13	13	CD
cord-007211-prygoc0q	141	6	]	]	-RRB-
cord-007211-prygoc0q	141	7	.	.	.
cord-007211-prygoc0q	142	1	This	this	DT
cord-007211-prygoc0q	142	2	indicated	indicate	VBD
cord-007211-prygoc0q	142	3	that	that	IN
cord-007211-prygoc0q	142	4	all	all	DT
cord-007211-prygoc0q	142	5	cysteine	cysteine	NN
cord-007211-prygoc0q	142	6	residues	residue	NNS
cord-007211-prygoc0q	142	7	contributed	contribute	VBD
cord-007211-prygoc0q	142	8	to	to	TO
cord-007211-prygoc0q	142	9	make	make	VB
cord-007211-prygoc0q	142	10	the	the	DT
cord-007211-prygoc0q	142	11	compact	compact	JJ
cord-007211-prygoc0q	142	12	form	form	NN
cord-007211-prygoc0q	142	13	.	.	.
cord-007211-prygoc0q	143	1	No	no	DT
cord-007211-prygoc0q	143	2	obvious	obvious	JJ
cord-007211-prygoc0q	143	3	oligomeric	oligomeric	JJ
cord-007211-prygoc0q	143	4	form	form	NN
cord-007211-prygoc0q	143	5	of	of	IN
cord-007211-prygoc0q	143	6	F	F	NNP
cord-007211-prygoc0q	143	7	proteins	protein	NNS
cord-007211-prygoc0q	143	8	was	be	VBD
cord-007211-prygoc0q	143	9	detected	detect	VBN
cord-007211-prygoc0q	143	10	in	in	IN
cord-007211-prygoc0q	143	11	the	the	DT
cord-007211-prygoc0q	143	12	mutant	mutant	JJ
cord-007211-prygoc0q	143	13	or	or	CC
cord-007211-prygoc0q	143	14	wild	wild	JJ
cord-007211-prygoc0q	143	15	-	-	HYPH
cord-007211-prygoc0q	143	16	type	type	NN
cord-007211-prygoc0q	143	17	F	F	NNP
cord-007211-prygoc0q	143	18	protein	protein	NN
cord-007211-prygoc0q	143	19	-	-	HYPH
cord-007211-prygoc0q	143	20	expressing	express	VBG
cord-007211-prygoc0q	143	21	cells	cell	NNS
cord-007211-prygoc0q	143	22	.	.	.
cord-007211-prygoc0q	144	1	This	this	DT
cord-007211-prygoc0q	144	2	implied	imply	VBD
cord-007211-prygoc0q	144	3	that	that	IN
cord-007211-prygoc0q	144	4	F	F	NNP
cord-007211-prygoc0q	144	5	protein	protein	NN
cord-007211-prygoc0q	144	6	does	do	VBZ
cord-007211-prygoc0q	144	7	not	not	RB
cord-007211-prygoc0q	144	8	form	form	VB
cord-007211-prygoc0q	144	9	disulfide	disulfide	RB
cord-007211-prygoc0q	144	10	-	-	HYPH
cord-007211-prygoc0q	144	11	linked	link	VBN
cord-007211-prygoc0q	144	12	oligomers	oligomer	NNS
cord-007211-prygoc0q	144	13	.	.	.
cord-007211-prygoc0q	145	1	Although	although	IN
cord-007211-prygoc0q	145	2	the	the	DT
cord-007211-prygoc0q	145	3	aggregates	aggregate	NNS
cord-007211-prygoc0q	145	4	disappeared	disappear	VBD
cord-007211-prygoc0q	145	5	from	from	IN
cord-007211-prygoc0q	145	6	the	the	DT
cord-007211-prygoc0q	145	7	top	top	NN
cord-007211-prygoc0q	145	8	of	of	IN
cord-007211-prygoc0q	145	9	the	the	DT
cord-007211-prygoc0q	145	10	gel	gel	NN
cord-007211-prygoc0q	145	11	when	when	WRB
cord-007211-prygoc0q	145	12	the	the	DT
cord-007211-prygoc0q	145	13	radiolabeled	radiolabeled	JJ
cord-007211-prygoc0q	145	14	cell	cell	NN
cord-007211-prygoc0q	145	15	lysates	lysate	NNS
cord-007211-prygoc0q	145	16	were	be	VBD
cord-007211-prygoc0q	145	17	reduced	reduce	VBN
cord-007211-prygoc0q	145	18	by	by	IN
cord-007211-prygoc0q	145	19	boiling	boil	VBG
cord-007211-prygoc0q	145	20	in	in	RP
cord-007211-prygoc0q	145	21	5	5	CD
cord-007211-prygoc0q	145	22	%	%	NN
cord-007211-prygoc0q	145	23	2-mercaptoethanol	2-mercaptoethanol	CD
cord-007211-prygoc0q	145	24	before	before	IN
cord-007211-prygoc0q	145	25	SDS	SDS	NNP
cord-007211-prygoc0q	145	26	-	-	HYPH
cord-007211-prygoc0q	145	27	PAGE	PAGE	NNP
cord-007211-prygoc0q	145	28	,	,	,
cord-007211-prygoc0q	145	29	treatment	treatment	NN
cord-007211-prygoc0q	145	30	with	with	IN
cord-007211-prygoc0q	145	31	thiol	thiol	NN
cord-007211-prygoc0q	145	32	alkylation	alkylation	NN
cord-007211-prygoc0q	145	33	reagents	reagent	NNS
cord-007211-prygoc0q	145	34	such	such	JJ
cord-007211-prygoc0q	145	35	as	as	IN
cord-007211-prygoc0q	145	36	iodoacetamide	iodoacetamide	NNP
cord-007211-prygoc0q	145	37	(	(	-LRB-
cord-007211-prygoc0q	145	38	IAA	IAA	NNP
cord-007211-prygoc0q	145	39	)	)	-RRB-
cord-007211-prygoc0q	145	40	or	or	CC
cord-007211-prygoc0q	145	41	iV	iv	CD
cord-007211-prygoc0q	145	42	-	-	HYPH
cord-007211-prygoc0q	145	43	ethylmaleimide	ethylmaleimide	NNP
cord-007211-prygoc0q	145	44	(	(	-LRB-
cord-007211-prygoc0q	145	45	NEM	NEM	NNP
cord-007211-prygoc0q	145	46	)	)	-RRB-
cord-007211-prygoc0q	145	47	had	have	VBD
cord-007211-prygoc0q	145	48	little	little	JJ
cord-007211-prygoc0q	145	49	effect	effect	NN
cord-007211-prygoc0q	145	50	on	on	IN
cord-007211-prygoc0q	145	51	the	the	DT
cord-007211-prygoc0q	145	52	formation	formation	NN
cord-007211-prygoc0q	145	53	of	of	IN
cord-007211-prygoc0q	145	54	the	the	DT
cord-007211-prygoc0q	145	55	aggregates	aggregate	NNS
cord-007211-prygoc0q	145	56	.	.	.
cord-007211-prygoc0q	146	1	In	in	IN
cord-007211-prygoc0q	146	2	preliminary	preliminary	JJ
cord-007211-prygoc0q	146	3	experiments	experiment	NNS
cord-007211-prygoc0q	146	4	,	,	,
cord-007211-prygoc0q	146	5	we	-PRON-	PRP
cord-007211-prygoc0q	146	6	did	do	VBD
cord-007211-prygoc0q	146	7	not	not	RB
cord-007211-prygoc0q	146	8	detect	detect	VB
cord-007211-prygoc0q	146	9	such	such	JJ
cord-007211-prygoc0q	146	10	aggregates	aggregate	NNS
cord-007211-prygoc0q	146	11	in	in	IN
cord-007211-prygoc0q	146	12	Sendai	Sendai	NNP
cord-007211-prygoc0q	146	13	virus	virus	NN
cord-007211-prygoc0q	146	14	-	-	HYPH
cord-007211-prygoc0q	146	15	infected	infect	VBN
cord-007211-prygoc0q	146	16	cells	cell	NNS
cord-007211-prygoc0q	146	17	analyzed	analyze	VBN
cord-007211-prygoc0q	146	18	under	under	IN
cord-007211-prygoc0q	146	19	nonreducing	nonreducing	NN
cord-007211-prygoc0q	146	20	conditions	condition	NNS
cord-007211-prygoc0q	146	21	.	.	.
cord-007211-prygoc0q	147	1	These	these	DT
cord-007211-prygoc0q	147	2	observations	observation	NNS
cord-007211-prygoc0q	147	3	indicated	indicate	VBD
cord-007211-prygoc0q	147	4	that	that	IN
cord-007211-prygoc0q	147	5	these	these	DT
cord-007211-prygoc0q	147	6	aggregates	aggregate	NNS
cord-007211-prygoc0q	147	7	were	be	VBD
cord-007211-prygoc0q	147	8	formed	form	VBN
cord-007211-prygoc0q	147	9	before	before	IN
cord-007211-prygoc0q	147	10	sample	sample	NN
cord-007211-prygoc0q	147	11	preparation	preparation	NN
cord-007211-prygoc0q	147	12	and	and	CC
cord-007211-prygoc0q	147	13	that	that	IN
cord-007211-prygoc0q	147	14	other	other	JJ
cord-007211-prygoc0q	147	15	viral	viral	JJ
cord-007211-prygoc0q	147	16	factors	factor	NNS
cord-007211-prygoc0q	147	17	might	may	MD
cord-007211-prygoc0q	147	18	be	be	VB
cord-007211-prygoc0q	147	19	required	require	VBN
cord-007211-prygoc0q	147	20	for	for	IN
cord-007211-prygoc0q	147	21	efficient	efficient	JJ
cord-007211-prygoc0q	147	22	folding	folding	NN
cord-007211-prygoc0q	147	23	of	of	IN
cord-007211-prygoc0q	147	24	F	F	NNP
cord-007211-prygoc0q	147	25	protein	protein	NN
cord-007211-prygoc0q	147	26	.	.	.
cord-007211-prygoc0q	148	1	Antibodies-	Antibodies-	NNP
cord-007211-prygoc0q	149	1	To	to	TO
cord-007211-prygoc0q	149	2	examine	examine	VB
cord-007211-prygoc0q	149	3	the	the	DT
cord-007211-prygoc0q	149	4	antigenicity	antigenicity	NN
cord-007211-prygoc0q	149	5	of	of	IN
cord-007211-prygoc0q	149	6	the	the	DT
cord-007211-prygoc0q	149	7	cysteine	cysteine	NN
cord-007211-prygoc0q	149	8	mutant	mutant	JJ
cord-007211-prygoc0q	149	9	proteins	protein	NNS
cord-007211-prygoc0q	149	10	,	,	,
cord-007211-prygoc0q	149	11	the	the	DT
cord-007211-prygoc0q	149	12	cell	cell	NN
cord-007211-prygoc0q	149	13	lysates	lysate	NNS
cord-007211-prygoc0q	149	14	were	be	VBD
cord-007211-prygoc0q	149	15	immunoprecipitated	immunoprecipitate	VBN
cord-007211-prygoc0q	149	16	with	with	IN
cord-007211-prygoc0q	149	17	MAbs	MAbs	NNP
cord-007211-prygoc0q	149	18	f-49	f-49	NNP
cord-007211-prygoc0q	149	19	,	,	,
cord-007211-prygoc0q	149	20	f-236	f-236	NNP
cord-007211-prygoc0q	149	21	,	,	,
cord-007211-prygoc0q	150	1	and	and	CC
cord-007211-prygoc0q	150	2	f-921	f-921	NNS
cord-007211-prygoc0q	150	3	and	and	CC
cord-007211-prygoc0q	150	4	analyzed	analyze	VBN
cord-007211-prygoc0q	150	5	by	by	IN
cord-007211-prygoc0q	150	6	SDS	SDS	NNP
cord-007211-prygoc0q	150	7	-	-	HYPH
cord-007211-prygoc0q	150	8	PAGE	PAGE	NNP
cord-007211-prygoc0q	150	9	under	under	IN
cord-007211-prygoc0q	150	10	reducing	reduce	VBG
cord-007211-prygoc0q	150	11	conditions	condition	NNS
cord-007211-prygoc0q	150	12	.	.	.
cord-007211-prygoc0q	151	1	In	in	IN
cord-007211-prygoc0q	151	2	preliminary	preliminary	JJ
cord-007211-prygoc0q	151	3	experiments	experiment	NNS
cord-007211-prygoc0q	151	4	,	,	,
cord-007211-prygoc0q	151	5	f-921	f-921	NNS
cord-007211-prygoc0q	151	6	detected	detect	VBD
cord-007211-prygoc0q	151	7	intracellular	intracellular	JJ
cord-007211-prygoc0q	151	8	F	F	NNP
cord-007211-prygoc0q	151	9	proteins	protein	NNS
cord-007211-prygoc0q	151	10	but	but	CC
cord-007211-prygoc0q	151	11	not	not	RB
cord-007211-prygoc0q	151	12	cell	cell	NN
cord-007211-prygoc0q	151	13	surface	surface	NN
cord-007211-prygoc0q	151	14	-	-	HYPH
cord-007211-prygoc0q	151	15	transported	transport	VBN
cord-007211-prygoc0q	151	16	F	F	NNP
cord-007211-prygoc0q	151	17	proteins	protein	NNS
cord-007211-prygoc0q	151	18	.	.	.
cord-007211-prygoc0q	152	1	MAb	MAb	NNS
cord-007211-prygoc0q	152	2	f-236	f-236	NNP
cord-007211-prygoc0q	152	3	recognized	recognize	VBD
cord-007211-prygoc0q	152	4	both	both	DT
cord-007211-prygoc0q	152	5	F	F	NNP
cord-007211-prygoc0q	152	6	o	o	NNP
cord-007211-prygoc0q	152	7	and	and	CC
cord-007211-prygoc0q	152	8	Fj	Fj	NNP
cord-007211-prygoc0q	152	9	in	in	IN
cord-007211-prygoc0q	152	10	the	the	DT
cord-007211-prygoc0q	152	11	immunoprecipitation	immunoprecipitation	NN
cord-007211-prygoc0q	152	12	,	,	,
cord-007211-prygoc0q	152	13	but	but	CC
cord-007211-prygoc0q	152	14	specifically	specifically	RB
cord-007211-prygoc0q	152	15	recognized	recognize	VBD
cord-007211-prygoc0q	152	16	F	F	NNP
cord-007211-prygoc0q	152	17	o	o	NN
cord-007211-prygoc0q	152	18	in	in	IN
cord-007211-prygoc0q	152	19	Western	western	JJ
cord-007211-prygoc0q	152	20	blot	blot	NN
cord-007211-prygoc0q	152	21	analysis	analysis	NN
cord-007211-prygoc0q	152	22	(	(	-LRB-
cord-007211-prygoc0q	152	23	unpublished	unpublished	JJ
cord-007211-prygoc0q	152	24	data	datum	NNS
cord-007211-prygoc0q	152	25	)	)	-RRB-
cord-007211-prygoc0q	152	26	.	.	.
cord-007211-prygoc0q	153	1	MAb	MAb	NNPS
cord-007211-prygoc0q	153	2	f-49	f-49	NNP
cord-007211-prygoc0q	153	3	recognized	recognize	VBD
cord-007211-prygoc0q	153	4	the	the	DT
cord-007211-prygoc0q	153	5	mature	mature	JJ
cord-007211-prygoc0q	153	6	form	form	NN
cord-007211-prygoc0q	153	7	of	of	IN
cord-007211-prygoc0q	153	8	F	F	NNP
cord-007211-prygoc0q	153	9	protein	protein	NN
cord-007211-prygoc0q	153	10	(	(	-LRB-
cord-007211-prygoc0q	153	11	24	24	CD
cord-007211-prygoc0q	153	12	)	)	-RRB-
cord-007211-prygoc0q	153	13	.	.	.
cord-007211-prygoc0q	154	1	Thus	thus	RB
cord-007211-prygoc0q	154	2	,	,	,
cord-007211-prygoc0q	154	3	these	these	DT
cord-007211-prygoc0q	154	4	MAbs	MAbs	NNP
cord-007211-prygoc0q	154	5	bind	bind	VB
cord-007211-prygoc0q	154	6	independent	independent	JJ
cord-007211-prygoc0q	154	7	epitopes	epitope	NNS
cord-007211-prygoc0q	154	8	.	.	.
cord-007211-prygoc0q	155	1	As	as	IN
cord-007211-prygoc0q	155	2	shown	show	VBN
cord-007211-prygoc0q	155	3	in	in	IN
cord-007211-prygoc0q	155	4	Fig	Fig	NNP
cord-007211-prygoc0q	155	5	.	.	.
cord-007211-prygoc0q	156	1	3	3	LS
cord-007211-prygoc0q	156	2	,	,	,
cord-007211-prygoc0q	156	3	only	only	RB
cord-007211-prygoc0q	156	4	the	the	DT
cord-007211-prygoc0q	156	5	wild	wild	JJ
cord-007211-prygoc0q	156	6	-	-	HYPH
cord-007211-prygoc0q	156	7	type	type	NN
cord-007211-prygoc0q	156	8	F	F	NNP
cord-007211-prygoc0q	156	9	protein	protein	NN
cord-007211-prygoc0q	156	10	was	be	VBD
cord-007211-prygoc0q	156	11	precipitated	precipitate	VBN
cord-007211-prygoc0q	156	12	with	with	IN
cord-007211-prygoc0q	156	13	anti	anti	JJ
cord-007211-prygoc0q	156	14	-	-	JJ
cord-007211-prygoc0q	156	15	mature	mature	JJ
cord-007211-prygoc0q	156	16	conformation	conformation	NN
cord-007211-prygoc0q	156	17	MAb	MAb	NNS
cord-007211-prygoc0q	156	18	f-49	f-49	NNP
cord-007211-prygoc0q	157	1	(	(	-LRB-
cord-007211-prygoc0q	157	2	panel	panel	NN
cord-007211-prygoc0q	157	3	A	A	NNP
cord-007211-prygoc0q	157	4	,	,	,
cord-007211-prygoc0q	157	5	lane	lane	NN
cord-007211-prygoc0q	157	6	3	3	CD
cord-007211-prygoc0q	157	7	)	)	-RRB-
cord-007211-prygoc0q	157	8	,	,	,
cord-007211-prygoc0q	157	9	the	the	DT
cord-007211-prygoc0q	157	10	wild	wild	JJ
cord-007211-prygoc0q	157	11	-	-	HYPH
cord-007211-prygoc0q	157	12	type	type	NN
cord-007211-prygoc0q	157	13	F	F	NNP
cord-007211-prygoc0q	157	14	protein	protein	NN
cord-007211-prygoc0q	157	15	and	and	CC
cord-007211-prygoc0q	157	16	a	a	DT
cord-007211-prygoc0q	157	17	small	small	JJ
cord-007211-prygoc0q	157	18	amount	amount	NN
cord-007211-prygoc0q	157	19	of	of	IN
cord-007211-prygoc0q	157	20	FC2S	FC2S	NNP
cord-007211-prygoc0q	157	21	protein	protein	NN
cord-007211-prygoc0q	157	22	were	be	VBD
cord-007211-prygoc0q	157	23	precipitated	precipitate	VBN
cord-007211-prygoc0q	157	24	with	with	IN
cord-007211-prygoc0q	157	25	MAb	MAb	NNS
cord-007211-prygoc0q	157	26	f-236	f-236	RB
cord-007211-prygoc0q	158	1	(	(	-LRB-
cord-007211-prygoc0q	158	2	panel	panel	NN
cord-007211-prygoc0q	158	3	B	B	NNP
cord-007211-prygoc0q	158	4	,	,	,
cord-007211-prygoc0q	158	5	lanes	lane	VBZ
cord-007211-prygoc0q	158	6	3	3	CD
cord-007211-prygoc0q	158	7	and	and	CC
cord-007211-prygoc0q	158	8	5	5	CD
cord-007211-prygoc0q	158	9	)	)	-RRB-
cord-007211-prygoc0q	158	10	,	,	,
cord-007211-prygoc0q	158	11	and	and	CC
cord-007211-prygoc0q	158	12	the	the	DT
cord-007211-prygoc0q	158	13	wild	wild	JJ
cord-007211-prygoc0q	158	14	-	-	HYPH
cord-007211-prygoc0q	158	15	type	type	NN
cord-007211-prygoc0q	158	16	F	F	NNP
cord-007211-prygoc0q	158	17	protein	protein	NN
cord-007211-prygoc0q	158	18	and	and	CC
cord-007211-prygoc0q	158	19	most	most	JJS
cord-007211-prygoc0q	158	20	of	of	IN
cord-007211-prygoc0q	158	21	the	the	DT
cord-007211-prygoc0q	158	22	cysteine	cysteine	NN
cord-007211-prygoc0q	158	23	mutant	mutant	NN
cord-007211-prygoc0q	158	24	F	F	NNP
cord-007211-prygoc0q	158	25	proteins	protein	NNS
cord-007211-prygoc0q	158	26	,	,	,
cord-007211-prygoc0q	158	27	except	except	IN
cord-007211-prygoc0q	158	28	for	for	IN
cord-007211-prygoc0q	158	29	the	the	DT
cord-007211-prygoc0q	158	30	FC5S	fc5s	JJ
cord-007211-prygoc0q	158	31	and	and	CC
cord-007211-prygoc0q	158	32	FC6S	FC6S	NNP
cord-007211-prygoc0q	158	33	proteins	protein	NNS
cord-007211-prygoc0q	158	34	,	,	,
cord-007211-prygoc0q	158	35	were	be	VBD
cord-007211-prygoc0q	158	36	precipitated	precipitate	VBN
cord-007211-prygoc0q	158	37	with	with	IN
cord-007211-prygoc0q	158	38	MAb	MAb	NNS
cord-007211-prygoc0q	158	39	f-921	f-921	NNS
cord-007211-prygoc0q	159	1	(	(	-LRB-
cord-007211-prygoc0q	159	2	panel	panel	NN
cord-007211-prygoc0q	159	3	C	C	NNP
cord-007211-prygoc0q	159	4	,	,	,
cord-007211-prygoc0q	159	5	lanes	lane	VBZ
cord-007211-prygoc0q	159	6	3	3	CD
cord-007211-prygoc0q	159	7	-	-	SYM
cord-007211-prygoc0q	159	8	7	7	CD
cord-007211-prygoc0q	159	9	and	and	CC
cord-007211-prygoc0q	159	10	10	10	CD
cord-007211-prygoc0q	159	11	-	-	SYM
cord-007211-prygoc0q	159	12	13	13	CD
cord-007211-prygoc0q	159	13	)	)	-RRB-
cord-007211-prygoc0q	159	14	.	.	.
cord-007211-prygoc0q	160	1	These	these	DT
cord-007211-prygoc0q	160	2	data	datum	NNS
cord-007211-prygoc0q	160	3	indicated	indicate	VBD
cord-007211-prygoc0q	160	4	that	that	IN
cord-007211-prygoc0q	160	5	loss	loss	NN
cord-007211-prygoc0q	160	6	of	of	IN
cord-007211-prygoc0q	160	7	any	any	DT
cord-007211-prygoc0q	160	8	one	one	CD
cord-007211-prygoc0q	160	9	of	of	IN
cord-007211-prygoc0q	160	10	the	the	DT
cord-007211-prygoc0q	160	11	cysteine	cysteine	NN
cord-007211-prygoc0q	160	12	residues	residue	NNS
cord-007211-prygoc0q	160	13	of	of	IN
cord-007211-prygoc0q	160	14	the	the	DT
cord-007211-prygoc0q	160	15	F	F	NNP
cord-007211-prygoc0q	160	16	protein	protein	NN
cord-007211-prygoc0q	160	17	had	have	VBD
cord-007211-prygoc0q	160	18	dramatic	dramatic	JJ
cord-007211-prygoc0q	160	19	effects	effect	NNS
cord-007211-prygoc0q	160	20	on	on	IN
cord-007211-prygoc0q	160	21	the	the	DT
cord-007211-prygoc0q	160	22	immunoreactivity	immunoreactivity	NN
cord-007211-prygoc0q	160	23	to	to	TO
cord-007211-prygoc0q	160	24	anti	anti	VB
cord-007211-prygoc0q	160	25	mature	mature	NNP
cord-007211-prygoc0q	160	26	MAbs	MAbs	NNP
cord-007211-prygoc0q	160	27	and	and	CC
cord-007211-prygoc0q	160	28	that	that	IN
cord-007211-prygoc0q	160	29	all	all	DT
cord-007211-prygoc0q	160	30	of	of	IN
cord-007211-prygoc0q	160	31	the	the	DT
cord-007211-prygoc0q	160	32	cysteine	cysteine	NN
cord-007211-prygoc0q	160	33	mutant	mutant	NN
cord-007211-prygoc0q	160	34	F	F	NNP
cord-007211-prygoc0q	160	35	proteins	protein	NNS
cord-007211-prygoc0q	160	36	were	be	VBD
cord-007211-prygoc0q	160	37	blocked	block	VBN
cord-007211-prygoc0q	160	38	at	at	IN
cord-007211-prygoc0q	160	39	some	some	DT
cord-007211-prygoc0q	160	40	stage	stage	NN
cord-007211-prygoc0q	160	41	in	in	IN
cord-007211-prygoc0q	160	42	their	-PRON-	PRP$
cord-007211-prygoc0q	160	43	maturation	maturation	NN
cord-007211-prygoc0q	160	44	.	.	.
cord-007211-prygoc0q	161	1	Cell	cell	NN
cord-007211-prygoc0q	161	2	Surface	Surface	NNP
cord-007211-prygoc0q	161	3	Transport	Transport	NNP
cord-007211-prygoc0q	161	4	of	of	IN
cord-007211-prygoc0q	161	5	Cysteine	Cysteine	NNP
cord-007211-prygoc0q	161	6	Mutant	Mutant	NNP
cord-007211-prygoc0q	161	7	F	F	NNP
cord-007211-prygoc0q	161	8	Proteins	Proteins	NNP
cord-007211-prygoc0q	161	9	-	-	:
cord-007211-prygoc0q	161	10	To	to	TO
cord-007211-prygoc0q	161	11	examine	examine	VB
cord-007211-prygoc0q	161	12	the	the	DT
cord-007211-prygoc0q	161	13	expression	expression	NN
cord-007211-prygoc0q	161	14	of	of	IN
cord-007211-prygoc0q	161	15	cysteine	cysteine	NN
cord-007211-prygoc0q	161	16	mutant	mutant	JJ
cord-007211-prygoc0q	161	17	F	F	NNP
cord-007211-prygoc0q	161	18	proteins	protein	NNS
cord-007211-prygoc0q	161	19	in	in	IN
cord-007211-prygoc0q	161	20	the	the	DT
cord-007211-prygoc0q	161	21	cells	cell	NNS
cord-007211-prygoc0q	161	22	and	and	CC
cord-007211-prygoc0q	161	23	their	-PRON-	PRP$
cord-007211-prygoc0q	161	24	transport	transport	NN
cord-007211-prygoc0q	161	25	to	to	IN
cord-007211-prygoc0q	161	26	the	the	DT
cord-007211-prygoc0q	161	27	cell	cell	NN
cord-007211-prygoc0q	161	28	surface	surface	NN
cord-007211-prygoc0q	161	29	,	,	,
cord-007211-prygoc0q	161	30	the	the	DT
cord-007211-prygoc0q	161	31	cells	cell	NNS
cord-007211-prygoc0q	161	32	were	be	VBD
cord-007211-prygoc0q	161	33	fixed	fix	VBN
cord-007211-prygoc0q	161	34	with	with	IN
cord-007211-prygoc0q	161	35	acetone	acetone	NN
cord-007211-prygoc0q	161	36	or	or	CC
cord-007211-prygoc0q	161	37	paraformaldehyde	paraformaldehyde	NN
cord-007211-prygoc0q	161	38	and	and	CC
cord-007211-prygoc0q	161	39	examined	examine	VBN
cord-007211-prygoc0q	161	40	by	by	IN
cord-007211-prygoc0q	161	41	indirect	indirect	JJ
cord-007211-prygoc0q	161	42	immunofluorescence	immunofluorescence	NN
cord-007211-prygoc0q	161	43	staining	staining	NN
cord-007211-prygoc0q	161	44	using	use	VBG
cord-007211-prygoc0q	161	45	anti	anti	JJ
cord-007211-prygoc0q	161	46	-	-	JJ
cord-007211-prygoc0q	161	47	F	f	JJ
cord-007211-prygoc0q	161	48	antiserum	antiserum	NN
cord-007211-prygoc0q	161	49	.	.	.
cord-007211-prygoc0q	162	1	To	to	TO
cord-007211-prygoc0q	162	2	detect	detect	VB
cord-007211-prygoc0q	162	3	subcellular	subcellular	JJ
cord-007211-prygoc0q	162	4	localization	localization	NN
cord-007211-prygoc0q	162	5	,	,	,
cord-007211-prygoc0q	162	6	the	the	DT
cord-007211-prygoc0q	162	7	cells	cell	NNS
cord-007211-prygoc0q	162	8	expressing	express	VBG
cord-007211-prygoc0q	162	9	wild	wild	JJ
cord-007211-prygoc0q	162	10	-	-	HYPH
cord-007211-prygoc0q	162	11	type	type	NN
cord-007211-prygoc0q	162	12	and	and	CC
cord-007211-prygoc0q	162	13	cysteine	cysteine	NN
cord-007211-prygoc0q	162	14	mutant	mutant	NN
cord-007211-prygoc0q	162	15	F	F	NNP
cord-007211-prygoc0q	162	16	proteins	protein	NNS
cord-007211-prygoc0q	162	17	were	be	VBD
cord-007211-prygoc0q	162	18	fixed	fix	VBN
cord-007211-prygoc0q	162	19	with	with	IN
cord-007211-prygoc0q	162	20	acetone	acetone	NN
cord-007211-prygoc0q	162	21	,	,	,
cord-007211-prygoc0q	162	22	then	then	RB
cord-007211-prygoc0q	162	23	examined	examine	VBN
cord-007211-prygoc0q	162	24	by	by	IN
cord-007211-prygoc0q	162	25	indirect	indirect	JJ
cord-007211-prygoc0q	162	26	immunofluorescence	immunofluorescence	NN
cord-007211-prygoc0q	162	27	staining	staining	NN
cord-007211-prygoc0q	162	28	(	(	-LRB-
cord-007211-prygoc0q	162	29	Fig	Fig	NNP
cord-007211-prygoc0q	162	30	.	.	NNP
cord-007211-prygoc0q	162	31	4	4	CD
cord-007211-prygoc0q	162	32	,	,	,
cord-007211-prygoc0q	162	33	panels	panel	NNS
cord-007211-prygoc0q	162	34	A	A	NNP
cord-007211-prygoc0q	162	35	,	,	,
cord-007211-prygoc0q	162	36	C	C	NNP
cord-007211-prygoc0q	162	37	,	,	,
cord-007211-prygoc0q	162	38	E	E	NNP
cord-007211-prygoc0q	162	39	,	,	,
cord-007211-prygoc0q	162	40	G	G	NNP
cord-007211-prygoc0q	162	41	,	,	,
cord-007211-prygoc0q	162	42	I	-PRON-	PRP
cord-007211-prygoc0q	162	43	,	,	,
cord-007211-prygoc0q	162	44	K	K	NNP
cord-007211-prygoc0q	162	45	,	,	,
cord-007211-prygoc0q	162	46	and	and	CC
cord-007211-prygoc0q	162	47	M	M	NNP
cord-007211-prygoc0q	162	48	)	)	-RRB-
cord-007211-prygoc0q	162	49	.	.	.
cord-007211-prygoc0q	163	1	We	-PRON-	PRP
cord-007211-prygoc0q	163	2	expected	expect	VBD
cord-007211-prygoc0q	163	3	that	that	IN
cord-007211-prygoc0q	163	4	a	a	DT
cord-007211-prygoc0q	163	5	pair	pair	NN
cord-007211-prygoc0q	163	6	of	of	IN
cord-007211-prygoc0q	163	7	cysteine	cysteine	NN
cord-007211-prygoc0q	163	8	mutants	mutant	NNS
cord-007211-prygoc0q	163	9	which	which	WDT
cord-007211-prygoc0q	163	10	had	have	VBD
cord-007211-prygoc0q	163	11	altered	alter	VBN
cord-007211-prygoc0q	163	12	cysteine	cysteine	NN
cord-007211-prygoc0q	163	13	residues	residue	NNS
cord-007211-prygoc0q	163	14	involving	involve	VBG
cord-007211-prygoc0q	163	15	the	the	DT
cord-007211-prygoc0q	163	16	same	same	JJ
cord-007211-prygoc0q	163	17	disulfide	disulfide	JJ
cord-007211-prygoc0q	163	18	bond	bond	NN
cord-007211-prygoc0q	163	19	might	may	MD
cord-007211-prygoc0q	163	20	show	show	VB
cord-007211-prygoc0q	163	21	the	the	DT
cord-007211-prygoc0q	163	22	same	same	JJ
cord-007211-prygoc0q	163	23	expression	expression	NN
cord-007211-prygoc0q	163	24	phenotype	phenotype	NN
cord-007211-prygoc0q	163	25	.	.	.
cord-007211-prygoc0q	164	1	Recent	recent	JJ
cord-007211-prygoc0q	164	2	results	result	NNS
cord-007211-prygoc0q	164	3	by	by	IN
cord-007211-prygoc0q	164	4	Iwata	Iwata	NNP
cord-007211-prygoc0q	164	5	et	et	NNP
cord-007211-prygoc0q	164	6	al	al	NNP
cord-007211-prygoc0q	164	7	.	.	.
cord-007211-prygoc0q	165	1	(	(	-LRB-
cord-007211-prygoc0q	165	2	15	15	CD
cord-007211-prygoc0q	165	3	)	)	-RRB-
cord-007211-prygoc0q	165	4	identified	identify	VBN
cord-007211-prygoc0q	165	5	specific	specific	JJ
cord-007211-prygoc0q	165	6	disulfide	disulfide	JJ
cord-007211-prygoc0q	165	7	bonds	bond	NNS
cord-007211-prygoc0q	165	8	between	between	IN
cord-007211-prygoc0q	165	9	Cl	Cl	NNP
cord-007211-prygoc0q	165	10	and	and	CC
cord-007211-prygoc0q	165	11	C2	C2	NNP
cord-007211-prygoc0q	165	12	,	,	,
cord-007211-prygoc0q	165	13	C3	C3	NNP
cord-007211-prygoc0q	165	14	and	and	CC
cord-007211-prygoc0q	165	15	C4	C4	NNP
cord-007211-prygoc0q	165	16	,	,	,
cord-007211-prygoc0q	165	17	and	and	CC
cord-007211-prygoc0q	165	18	C5	C5	NNP
cord-007211-prygoc0q	165	19	and	and	CC
cord-007211-prygoc0q	165	20	C6	C6	NNP
cord-007211-prygoc0q	165	21	,	,	,
cord-007211-prygoc0q	165	22	while	while	IN
cord-007211-prygoc0q	165	23	CIO	CIO	NNP
cord-007211-prygoc0q	165	24	appears	appear	VBZ
cord-007211-prygoc0q	165	25	to	to	TO
cord-007211-prygoc0q	165	26	be	be	VB
cord-007211-prygoc0q	165	27	linked	link	VBN
cord-007211-prygoc0q	165	28	to	to	IN
cord-007211-prygoc0q	165	29	C7	C7	NNP
cord-007211-prygoc0q	165	30	,	,	,
cord-007211-prygoc0q	165	31	C8	c8	NN
cord-007211-prygoc0q	165	32	,	,	,
cord-007211-prygoc0q	165	33	or	or	CC
cord-007211-prygoc0q	165	34	C9	C9	NNP
cord-007211-prygoc0q	165	35	.	.	.
cord-007211-prygoc0q	166	1	Thus	thus	RB
cord-007211-prygoc0q	166	2	,	,	,
cord-007211-prygoc0q	166	3	only	only	RB
cord-007211-prygoc0q	166	4	the	the	DT
cord-007211-prygoc0q	166	5	5	5	CD
cord-007211-prygoc0q	166	6	cysteine	cysteine	NN
cord-007211-prygoc0q	166	7	mutants	mutant	NNS
cord-007211-prygoc0q	167	1	FC1S	fc1	NNS
cord-007211-prygoc0q	167	2	,	,	,
cord-007211-prygoc0q	167	3	FC3S	FC3S	NNP
cord-007211-prygoc0q	167	4	,	,	,
cord-007211-prygoc0q	167	5	FC5S	FC5S	NNP
cord-007211-prygoc0q	167	6	,	,	,
cord-007211-prygoc0q	167	7	FC7S	FC7S	NNP
cord-007211-prygoc0q	167	8	,	,	,
cord-007211-prygoc0q	167	9	and	and	CC
cord-007211-prygoc0q	168	1	FC8S	FC8S	NNP
cord-007211-prygoc0q	168	2	were	be	VBD
cord-007211-prygoc0q	168	3	shown	show	VBN
cord-007211-prygoc0q	168	4	in	in	IN
cord-007211-prygoc0q	168	5	Fig	Fig	NNP
cord-007211-prygoc0q	168	6	.	.	.
cord-007211-prygoc0q	169	1	4	4	LS
cord-007211-prygoc0q	169	2	.	.	.
cord-007211-prygoc0q	170	1	The	the	DT
cord-007211-prygoc0q	170	2	COS	COS	NNP
cord-007211-prygoc0q	170	3	cells	cell	NNS
cord-007211-prygoc0q	170	4	transfected	transfecte	VBN
cord-007211-prygoc0q	170	5	with	with	IN
cord-007211-prygoc0q	170	6	vector	vector	NN
cord-007211-prygoc0q	170	7	plasmid	plasmid	NN
cord-007211-prygoc0q	170	8	pSRD	psrd	NN
cord-007211-prygoc0q	170	9	showed	show	VBD
cord-007211-prygoc0q	170	10	no	no	DT
cord-007211-prygoc0q	170	11	fluorescence	fluorescence	NN
cord-007211-prygoc0q	170	12	(	(	-LRB-
cord-007211-prygoc0q	170	13	panel	panel	NN
cord-007211-prygoc0q	170	14	A	A	NNP
cord-007211-prygoc0q	170	15	)	)	-RRB-
cord-007211-prygoc0q	170	16	,	,	,
cord-007211-prygoc0q	170	17	whereas	whereas	IN
cord-007211-prygoc0q	170	18	the	the	DT
cord-007211-prygoc0q	170	19	cells	cell	NNS
cord-007211-prygoc0q	170	20	transfected	transfecte	VBN
cord-007211-prygoc0q	170	21	with	with	IN
cord-007211-prygoc0q	170	22	pSRD	psrd	CD
cord-007211-prygoc0q	170	23	-	-	HYPH
cord-007211-prygoc0q	170	24	F	f	NN
cord-007211-prygoc0q	170	25	(	(	-LRB-
cord-007211-prygoc0q	170	26	panel	panel	NN
cord-007211-prygoc0q	170	27	C	C	NNP
cord-007211-prygoc0q	170	28	)	)	-RRB-
cord-007211-prygoc0q	170	29	,	,	,
cord-007211-prygoc0q	170	30	pSRD	pSRD	NNP
cord-007211-prygoc0q	170	31	-	-	HYPH
cord-007211-prygoc0q	170	32	FClS	fcls	JJ
cord-007211-prygoc0q	170	33	(	(	-LRB-
cord-007211-prygoc0q	170	34	panel	panel	NN
cord-007211-prygoc0q	170	35	E	E	NNP
cord-007211-prygoc0q	170	36	)	)	-RRB-
cord-007211-prygoc0q	170	37	,	,	,
cord-007211-prygoc0q	170	38	pSRD	pSRD	VBN
cord-007211-prygoc0q	170	39	-	-	HYPH
cord-007211-prygoc0q	170	40	FC3S	fc3s	NN
cord-007211-prygoc0q	170	41	(	(	-LRB-
cord-007211-prygoc0q	170	42	panel	panel	NN
cord-007211-prygoc0q	170	43	G	G	NNP
cord-007211-prygoc0q	170	44	)	)	-RRB-
cord-007211-prygoc0q	170	45	,	,	,
cord-007211-prygoc0q	170	46	pSRD	psrd	NN
cord-007211-prygoc0q	170	47	-	-	HYPH
cord-007211-prygoc0q	170	48	FC5S	fc5s	NN
cord-007211-prygoc0q	170	49	(	(	-LRB-
cord-007211-prygoc0q	170	50	panel	panel	NN
cord-007211-prygoc0q	170	51	I	I	NNP
cord-007211-prygoc0q	170	52	)	)	-RRB-
cord-007211-prygoc0q	170	53	,	,	,
cord-007211-prygoc0q	170	54	pSRD	pSRD	NNP
cord-007211-prygoc0q	170	55	-	-	HYPH
cord-007211-prygoc0q	170	56	FC7S	FC7S	NNP
cord-007211-prygoc0q	170	57	(	(	-LRB-
cord-007211-prygoc0q	170	58	panel	panel	NN
cord-007211-prygoc0q	170	59	K	K	NNP
cord-007211-prygoc0q	170	60	)	)	-RRB-
cord-007211-prygoc0q	170	61	,	,	,
cord-007211-prygoc0q	170	62	and	and	CC
cord-007211-prygoc0q	170	63	pSRD	psrd	CD
cord-007211-prygoc0q	170	64	-	-	HYPH
cord-007211-prygoc0q	170	65	FC8S	FC8S	NNP
cord-007211-prygoc0q	170	66	(	(	-LRB-
cord-007211-prygoc0q	170	67	panel	panel	NN
cord-007211-prygoc0q	170	68	M	M	NNP
cord-007211-prygoc0q	170	69	)	)	-RRB-
cord-007211-prygoc0q	170	70	displayed	display	VBD
cord-007211-prygoc0q	170	71	a	a	DT
cord-007211-prygoc0q	170	72	bright	bright	JJ
cord-007211-prygoc0q	170	73	staining	staining	NN
cord-007211-prygoc0q	170	74	pattern	pattern	NN
cord-007211-prygoc0q	170	75	,	,	,
cord-007211-prygoc0q	170	76	and	and	CC
cord-007211-prygoc0q	170	77	the	the	DT
cord-007211-prygoc0q	170	78	frequency	frequency	NN
cord-007211-prygoc0q	170	79	of	of	IN
cord-007211-prygoc0q	170	80	positive	positive	JJ
cord-007211-prygoc0q	170	81	cells	cell	NNS
cord-007211-prygoc0q	170	82	was	be	VBD
cord-007211-prygoc0q	170	83	estimated	estimate	VBN
cord-007211-prygoc0q	170	84	to	to	TO
cord-007211-prygoc0q	170	85	be	be	VB
cord-007211-prygoc0q	170	86	5	5	CD
cord-007211-prygoc0q	170	87	-	-	SYM
cord-007211-prygoc0q	170	88	10	10	CD
cord-007211-prygoc0q	170	89	%	%	NN
cord-007211-prygoc0q	170	90	of	of	IN
cord-007211-prygoc0q	170	91	total	total	JJ
cord-007211-prygoc0q	170	92	cells	cell	NNS
cord-007211-prygoc0q	170	93	in	in	IN
cord-007211-prygoc0q	170	94	each	each	DT
cord-007211-prygoc0q	170	95	case	case	NN
cord-007211-prygoc0q	170	96	.	.	.
cord-007211-prygoc0q	171	1	As	as	IN
cord-007211-prygoc0q	171	2	shown	show	VBN
cord-007211-prygoc0q	171	3	in	in	IN
cord-007211-prygoc0q	171	4	panel	panel	NN
cord-007211-prygoc0q	171	5	C	C	NNP
cord-007211-prygoc0q	171	6	,	,	,
cord-007211-prygoc0q	171	7	the	the	DT
cord-007211-prygoc0q	171	8	cells	cell	NNS
cord-007211-prygoc0q	171	9	expressing	express	VBG
cord-007211-prygoc0q	171	10	wild	wild	JJ
cord-007211-prygoc0q	171	11	-	-	HYPH
cord-007211-prygoc0q	171	12	type	type	NN
cord-007211-prygoc0q	171	13	F	F	NNP
cord-007211-prygoc0q	171	14	protein	protein	NN
cord-007211-prygoc0q	171	15	displayed	display	VBD
cord-007211-prygoc0q	171	16	internal	internal	JJ
cord-007211-prygoc0q	171	17	staining	staining	NN
cord-007211-prygoc0q	171	18	throughout	throughout	IN
cord-007211-prygoc0q	171	19	the	the	DT
cord-007211-prygoc0q	171	20	cytoplasmic	cytoplasmic	JJ
cord-007211-prygoc0q	171	21	reticulum	reticulum	NN
cord-007211-prygoc0q	171	22	as	as	RB
cord-007211-prygoc0q	171	23	well	well	RB
cord-007211-prygoc0q	171	24	as	as	IN
cord-007211-prygoc0q	171	25	in	in	IN
cord-007211-prygoc0q	171	26	the	the	DT
cord-007211-prygoc0q	171	27	juxtanuclear	juxtanuclear	NNP
cord-007211-prygoc0q	171	28	region	region	NN
cord-007211-prygoc0q	171	29	.	.	.
cord-007211-prygoc0q	172	1	On	on	IN
cord-007211-prygoc0q	172	2	the	the	DT
cord-007211-prygoc0q	172	3	other	other	JJ
cord-007211-prygoc0q	172	4	hand	hand	NN
cord-007211-prygoc0q	172	5	,	,	,
cord-007211-prygoc0q	172	6	the	the	DT
cord-007211-prygoc0q	172	7	cysteine	cysteine	NN
cord-007211-prygoc0q	172	8	mutant	mutant	NN
cord-007211-prygoc0q	172	9	-	-	HYPH
cord-007211-prygoc0q	172	10	expressing	express	VBG
cord-007211-prygoc0q	172	11	cells	cell	NNS
cord-007211-prygoc0q	172	12	showed	show	VBD
cord-007211-prygoc0q	172	13	an	an	DT
cord-007211-prygoc0q	172	14	intracellular	intracellular	JJ
cord-007211-prygoc0q	172	15	staining	staining	NN
cord-007211-prygoc0q	172	16	pattern	pattern	NN
cord-007211-prygoc0q	172	17	limited	limit	VBN
cord-007211-prygoc0q	172	18	to	to	IN
cord-007211-prygoc0q	172	19	a	a	DT
cord-007211-prygoc0q	172	20	reticular	reticular	JJ
cord-007211-prygoc0q	172	21	perinuclear	perinuclear	NN
cord-007211-prygoc0q	172	22	structure	structure	NN
cord-007211-prygoc0q	172	23	(	(	-LRB-
cord-007211-prygoc0q	172	24	panels	panel	NNS
cord-007211-prygoc0q	172	25	E	e	NN
cord-007211-prygoc0q	172	26	,	,	,
cord-007211-prygoc0q	172	27	G	g	NN
cord-007211-prygoc0q	172	28	,	,	,
cord-007211-prygoc0q	172	29	I	-PRON-	PRP
cord-007211-prygoc0q	172	30	,	,	,
cord-007211-prygoc0q	172	31	K	K	NNP
cord-007211-prygoc0q	172	32	,	,	,
cord-007211-prygoc0q	172	33	and	and	CC
cord-007211-prygoc0q	172	34	M	M	NNP
cord-007211-prygoc0q	172	35	)	)	-RRB-
cord-007211-prygoc0q	172	36	.	.	.
cord-007211-prygoc0q	173	1	This	this	DT
cord-007211-prygoc0q	173	2	indicates	indicate	VBZ
cord-007211-prygoc0q	173	3	that	that	IN
cord-007211-prygoc0q	173	4	cysteine	cysteine	NN
cord-007211-prygoc0q	173	5	mutant	mutant	JJ
cord-007211-prygoc0q	173	6	F	F	NNP
cord-007211-prygoc0q	173	7	proteins	protein	NNS
cord-007211-prygoc0q	173	8	were	be	VBD
cord-007211-prygoc0q	173	9	efficiently	efficiently	RB
cord-007211-prygoc0q	173	10	expressed	express	VBN
cord-007211-prygoc0q	173	11	in	in	IN
cord-007211-prygoc0q	173	12	these	these	DT
cord-007211-prygoc0q	173	13	cells	cell	NNS
cord-007211-prygoc0q	173	14	.	.	.
cord-007211-prygoc0q	174	1	To	to	TO
cord-007211-prygoc0q	174	2	detect	detect	VB
cord-007211-prygoc0q	174	3	whether	whether	IN
cord-007211-prygoc0q	174	4	the	the	DT
cord-007211-prygoc0q	174	5	expressed	express	VBN
cord-007211-prygoc0q	174	6	proteins	protein	NNS
cord-007211-prygoc0q	174	7	were	be	VBD
cord-007211-prygoc0q	174	8	transported	transport	VBN
cord-007211-prygoc0q	174	9	to	to	IN
cord-007211-prygoc0q	174	10	the	the	DT
cord-007211-prygoc0q	174	11	cell	cell	NN
cord-007211-prygoc0q	174	12	surface	surface	NN
cord-007211-prygoc0q	174	13	,	,	,
cord-007211-prygoc0q	174	14	the	the	DT
cord-007211-prygoc0q	174	15	cells	cell	NNS
cord-007211-prygoc0q	174	16	were	be	VBD
cord-007211-prygoc0q	174	17	fixed	fix	VBN
cord-007211-prygoc0q	174	18	with	with	IN
cord-007211-prygoc0q	174	19	paraformaldehyde	paraformaldehyde	NNP
cord-007211-prygoc0q	174	20	instead	instead	RB
cord-007211-prygoc0q	174	21	of	of	IN
cord-007211-prygoc0q	174	22	acetone	acetone	NN
cord-007211-prygoc0q	174	23	,	,	,
cord-007211-prygoc0q	174	24	then	then	RB
cord-007211-prygoc0q	174	25	examined	examine	VBN
cord-007211-prygoc0q	174	26	by	by	IN
cord-007211-prygoc0q	174	27	indirect	indirect	JJ
cord-007211-prygoc0q	174	28	immunofluorescence	immunofluorescence	NN
cord-007211-prygoc0q	174	29	staining	staining	NN
cord-007211-prygoc0q	174	30	.	.	.
cord-007211-prygoc0q	175	1	The	the	DT
cord-007211-prygoc0q	175	2	cells	cell	NNS
cord-007211-prygoc0q	175	3	transfected	transfecte	VBN
cord-007211-prygoc0q	175	4	with	with	IN
cord-007211-prygoc0q	175	5	pSRD	psrd	CD
cord-007211-prygoc0q	175	6	-	-	HYPH
cord-007211-prygoc0q	175	7	F	f	NN
cord-007211-prygoc0q	175	8	displayed	display	VBD
cord-007211-prygoc0q	175	9	a	a	DT
cord-007211-prygoc0q	175	10	staining	staining	NN
cord-007211-prygoc0q	175	11	pattern	pattern	NN
cord-007211-prygoc0q	175	12	on	on	IN
cord-007211-prygoc0q	175	13	the	the	DT
cord-007211-prygoc0q	175	14	cell	cell	NN
cord-007211-prygoc0q	175	15	surface	surface	NN
cord-007211-prygoc0q	175	16	(	(	-LRB-
cord-007211-prygoc0q	175	17	panel	panel	NN
cord-007211-prygoc0q	175	18	B	B	NNP
cord-007211-prygoc0q	175	19	)	)	-RRB-
cord-007211-prygoc0q	175	20	,	,	,
cord-007211-prygoc0q	175	21	whereas	whereas	IN
cord-007211-prygoc0q	175	22	the	the	DT
cord-007211-prygoc0q	175	23	surface	surface	NN
cord-007211-prygoc0q	175	24	fluorescence	fluorescence	NN
cord-007211-prygoc0q	175	25	of	of	IN
cord-007211-prygoc0q	175	26	the	the	DT
cord-007211-prygoc0q	175	27	cells	cell	NNS
cord-007211-prygoc0q	175	28	transfected	transfecte	VBN
cord-007211-prygoc0q	175	29	with	with	IN
cord-007211-prygoc0q	175	30	the	the	DT
cord-007211-prygoc0q	175	31	cysteine	cysteine	NN
cord-007211-prygoc0q	175	32	mutant	mutant	NN
cord-007211-prygoc0q	175	33	F	F	NNP
cord-007211-prygoc0q	175	34	plasmids	plasmid	NNS
cord-007211-prygoc0q	175	35	was	be	VBD
cord-007211-prygoc0q	175	36	clearly	clearly	RB
cord-007211-prygoc0q	175	37	much	much	RB
cord-007211-prygoc0q	175	38	less	less	RBR
cord-007211-prygoc0q	175	39	intense	intense	JJ
cord-007211-prygoc0q	175	40	than	than	IN
cord-007211-prygoc0q	175	41	that	that	DT
cord-007211-prygoc0q	175	42	of	of	IN
cord-007211-prygoc0q	175	43	cells	cell	NNS
cord-007211-prygoc0q	175	44	expressing	express	VBG
cord-007211-prygoc0q	175	45	wild	wild	JJ
cord-007211-prygoc0q	175	46	-	-	HYPH
cord-007211-prygoc0q	175	47	type	type	NN
cord-007211-prygoc0q	175	48	F	f	NN
cord-007211-prygoc0q	175	49	protein	protein	NN
cord-007211-prygoc0q	175	50	(	(	-LRB-
cord-007211-prygoc0q	175	51	panels	panel	NNS
cord-007211-prygoc0q	175	52	D	D	NNP
cord-007211-prygoc0q	175	53	,	,	,
cord-007211-prygoc0q	175	54	F	F	NNP
cord-007211-prygoc0q	175	55	,	,	,
cord-007211-prygoc0q	175	56	H	H	NNP
cord-007211-prygoc0q	175	57	,	,	,
cord-007211-prygoc0q	175	58	J	J	NNP
cord-007211-prygoc0q	175	59	,	,	,
cord-007211-prygoc0q	175	60	and	and	CC
cord-007211-prygoc0q	175	61	L	L	NNP
cord-007211-prygoc0q	175	62	)	)	-RRB-
cord-007211-prygoc0q	175	63	.	.	.
cord-007211-prygoc0q	176	1	This	this	DT
cord-007211-prygoc0q	176	2	indicates	indicate	VBZ
cord-007211-prygoc0q	176	3	that	that	IN
cord-007211-prygoc0q	176	4	the	the	DT
cord-007211-prygoc0q	176	5	wild	wild	JJ
cord-007211-prygoc0q	176	6	-	-	HYPH
cord-007211-prygoc0q	176	7	type	type	NN
cord-007211-prygoc0q	176	8	F	F	NNP
cord-007211-prygoc0q	176	9	protein	protein	NN
cord-007211-prygoc0q	176	10	was	be	VBD
cord-007211-prygoc0q	176	11	properly	properly	RB
cord-007211-prygoc0q	176	12	transported	transport	VBN
cord-007211-prygoc0q	176	13	to	to	IN
cord-007211-prygoc0q	176	14	the	the	DT
cord-007211-prygoc0q	176	15	cell	cell	NN
cord-007211-prygoc0q	176	16	surface	surface	NN
cord-007211-prygoc0q	176	17	,	,	,
cord-007211-prygoc0q	176	18	but	but	CC
cord-007211-prygoc0q	176	19	these	these	DT
cord-007211-prygoc0q	176	20	cysteine	cysteine	NN
cord-007211-prygoc0q	176	21	mutant	mutant	NN
cord-007211-prygoc0q	176	22	F	F	NNP
cord-007211-prygoc0q	176	23	proteins	protein	NNS
cord-007211-prygoc0q	176	24	were	be	VBD
cord-007211-prygoc0q	176	25	not	not	RB
cord-007211-prygoc0q	176	26	transported	transport	VBN
cord-007211-prygoc0q	176	27	to	to	IN
cord-007211-prygoc0q	176	28	the	the	DT
cord-007211-prygoc0q	176	29	cell	cell	NN
cord-007211-prygoc0q	176	30	surface	surface	NN
cord-007211-prygoc0q	176	31	.	.	.
cord-007211-prygoc0q	177	1	In	in	IN
cord-007211-prygoc0q	177	2	Sendai	Sendai	NNP
cord-007211-prygoc0q	177	3	virus	virus	NN
cord-007211-prygoc0q	177	4	-	-	HYPH
cord-007211-prygoc0q	177	5	infected	infect	VBN
cord-007211-prygoc0q	177	6	cells	cell	NNS
cord-007211-prygoc0q	177	7	,	,	,
cord-007211-prygoc0q	177	8	both	both	CC
cord-007211-prygoc0q	177	9	F	F	NNP
cord-007211-prygoc0q	177	10	protein	protein	NN
cord-007211-prygoc0q	177	11	and	and	CC
cord-007211-prygoc0q	177	12	HN	HN	NNP
cord-007211-prygoc0q	177	13	protein	protein	NN
cord-007211-prygoc0q	177	14	are	be	VBP
cord-007211-prygoc0q	177	15	transported	transport	VBN
cord-007211-prygoc0q	177	16	to	to	IN
cord-007211-prygoc0q	177	17	the	the	DT
cord-007211-prygoc0q	177	18	cell	cell	NN
cord-007211-prygoc0q	177	19	surface	surface	NN
cord-007211-prygoc0q	177	20	.	.	.
cord-007211-prygoc0q	178	1	To	to	TO
cord-007211-prygoc0q	178	2	examine	examine	VB
cord-007211-prygoc0q	178	3	the	the	DT
cord-007211-prygoc0q	178	4	effect	effect	NN
cord-007211-prygoc0q	178	5	of	of	IN
cord-007211-prygoc0q	178	6	HN	HN	NNP
cord-007211-prygoc0q	178	7	on	on	IN
cord-007211-prygoc0q	178	8	cell	cell	NN
cord-007211-prygoc0q	178	9	surface	surface	NN
cord-007211-prygoc0q	178	10	expression	expression	NN
cord-007211-prygoc0q	178	11	of	of	IN
cord-007211-prygoc0q	178	12	wild	wild	JJ
cord-007211-prygoc0q	178	13	-	-	HYPH
cord-007211-prygoc0q	178	14	type	type	NN
cord-007211-prygoc0q	178	15	and	and	CC
cord-007211-prygoc0q	178	16	cysteine	cysteine	NN
cord-007211-prygoc0q	178	17	mutant	mutant	NN
cord-007211-prygoc0q	178	18	F	F	NNP
cord-007211-prygoc0q	178	19	proteins	protein	NNS
cord-007211-prygoc0q	178	20	,	,	,
cord-007211-prygoc0q	178	21	cells	cell	NNS
cord-007211-prygoc0q	178	22	co	co	VBD
cord-007211-prygoc0q	178	23	-	-	VBN
cord-007211-prygoc0q	178	24	transfected	transfecte	VBN
cord-007211-prygoc0q	179	1	with	with	IN
cord-007211-prygoc0q	179	2	the	the	DT
cord-007211-prygoc0q	179	3	HN	HN	NNP
cord-007211-prygoc0q	179	4	gene	gene	NN
cord-007211-prygoc0q	179	5	and	and	CC
cord-007211-prygoc0q	179	6	wild	wild	JJ
cord-007211-prygoc0q	179	7	-	-	HYPH
cord-007211-prygoc0q	179	8	type	type	NN
cord-007211-prygoc0q	179	9	F	f	NN
cord-007211-prygoc0q	179	10	or	or	CC
cord-007211-prygoc0q	179	11	one	one	CD
cord-007211-prygoc0q	179	12	of	of	IN
cord-007211-prygoc0q	179	13	the	the	DT
cord-007211-prygoc0q	179	14	cysteine	cysteine	NN
cord-007211-prygoc0q	179	15	mutant	mutant	NN
cord-007211-prygoc0q	179	16	F	F	NNP
cord-007211-prygoc0q	179	17	genes	gene	NNS
cord-007211-prygoc0q	179	18	were	be	VBD
cord-007211-prygoc0q	179	19	fixed	fix	VBN
cord-007211-prygoc0q	179	20	with	with	IN
cord-007211-prygoc0q	179	21	paraformaldehyde	paraformaldehyde	NNP
cord-007211-prygoc0q	179	22	,	,	,
cord-007211-prygoc0q	179	23	then	then	RB
cord-007211-prygoc0q	179	24	cell	cell	NN
cord-007211-prygoc0q	179	25	surface	surface	NN
cord-007211-prygoc0q	179	26	expression	expression	NN
cord-007211-prygoc0q	179	27	of	of	IN
cord-007211-prygoc0q	179	28	F	F	NNP
cord-007211-prygoc0q	179	29	or	or	CC
cord-007211-prygoc0q	179	30	HN	HN	NNP
cord-007211-prygoc0q	179	31	proteins	protein	NNS
cord-007211-prygoc0q	179	32	was	be	VBD
cord-007211-prygoc0q	179	33	tested	test	VBN
cord-007211-prygoc0q	179	34	with	with	IN
cord-007211-prygoc0q	179	35	anti	anti	JJ
cord-007211-prygoc0q	179	36	-	-	JJ
cord-007211-prygoc0q	179	37	F	F	NNP
cord-007211-prygoc0q	179	38	antiserum	antiserum	NN
cord-007211-prygoc0q	179	39	or	or	CC
cord-007211-prygoc0q	179	40	anti	anti	JJ
cord-007211-prygoc0q	179	41	-	-	JJ
cord-007211-prygoc0q	179	42	HN	hn	JJ
cord-007211-prygoc0q	179	43	MAb	MAb	NNS
cord-007211-prygoc0q	180	1	yl-111	yl-111	NNP
cord-007211-prygoc0q	180	2	.	.	.
cord-007211-prygoc0q	181	1	The	the	DT
cord-007211-prygoc0q	181	2	cell	cell	NN
cord-007211-prygoc0q	181	3	surface	surface	NN
cord-007211-prygoc0q	181	4	expression	expression	NN
cord-007211-prygoc0q	181	5	of	of	IN
cord-007211-prygoc0q	181	6	cysteine	cysteine	NN
cord-007211-prygoc0q	181	7	mutant	mutant	JJ
cord-007211-prygoc0q	181	8	F	F	NNP
cord-007211-prygoc0q	181	9	proteins	protein	NNS
cord-007211-prygoc0q	181	10	was	be	VBD
cord-007211-prygoc0q	181	11	not	not	RB
cord-007211-prygoc0q	181	12	detected	detect	VBN
cord-007211-prygoc0q	181	13	(	(	-LRB-
cord-007211-prygoc0q	181	14	data	datum	NNS
cord-007211-prygoc0q	181	15	not	not	RB
cord-007211-prygoc0q	181	16	shown	show	VBN
cord-007211-prygoc0q	181	17	)	)	-RRB-
cord-007211-prygoc0q	181	18	.	.	.
cord-007211-prygoc0q	182	1	Furthermore	furthermore	RB
cord-007211-prygoc0q	182	2	,	,	,
cord-007211-prygoc0q	182	3	as	as	IN
cord-007211-prygoc0q	182	4	shown	show	VBN
cord-007211-prygoc0q	182	5	in	in	IN
cord-007211-prygoc0q	182	6	Fig	Fig	NNP
cord-007211-prygoc0q	182	7	.	.	.
cord-007211-prygoc0q	183	1	5A	5a	NN
cord-007211-prygoc0q	183	2	,	,	,
cord-007211-prygoc0q	183	3	the	the	DT
cord-007211-prygoc0q	183	4	cell	cell	NN
cord-007211-prygoc0q	183	5	surface	surface	NN
cord-007211-prygoc0q	183	6	expression	expression	NN
cord-007211-prygoc0q	183	7	of	of	IN
cord-007211-prygoc0q	183	8	the	the	DT
cord-007211-prygoc0q	183	9	HN	HN	NNP
cord-007211-prygoc0q	183	10	protein	protein	NN
cord-007211-prygoc0q	183	11	was	be	VBD
cord-007211-prygoc0q	183	12	drastically	drastically	RB
cord-007211-prygoc0q	183	13	reduced	reduce	VBN
cord-007211-prygoc0q	183	14	in	in	IN
cord-007211-prygoc0q	183	15	the	the	DT
cord-007211-prygoc0q	183	16	cells	cell	NNS
cord-007211-prygoc0q	183	17	co	co	VBD
cord-007211-prygoc0q	183	18	-	-	JJ
cord-007211-prygoc0q	183	19	expressing	express	VBG
cord-007211-prygoc0q	183	20	HN	HN	NNP
cord-007211-prygoc0q	183	21	and	and	CC
cord-007211-prygoc0q	183	22	cysteine	cysteine	NN
cord-007211-prygoc0q	183	23	mutant	mutant	NN
cord-007211-prygoc0q	183	24	F	F	NNP
cord-007211-prygoc0q	183	25	proteins	protein	NNS
cord-007211-prygoc0q	183	26	.	.	.
cord-007211-prygoc0q	184	1	In	in	IN
cord-007211-prygoc0q	184	2	panels	panel	NNS
cord-007211-prygoc0q	184	3	(	(	-LRB-
cord-007211-prygoc0q	184	4	b	b	NN
cord-007211-prygoc0q	184	5	)	)	-RRB-
cord-007211-prygoc0q	184	6	and	and	CC
cord-007211-prygoc0q	184	7	(	(	-LRB-
cord-007211-prygoc0q	184	8	c	c	NN
cord-007211-prygoc0q	184	9	)	)	-RRB-
cord-007211-prygoc0q	184	10	,	,	,
cord-007211-prygoc0q	184	11	HN	HN	NNP
cord-007211-prygoc0q	184	12	protein	protein	NN
cord-007211-prygoc0q	184	13	was	be	VBD
cord-007211-prygoc0q	184	14	efficiently	efficiently	RB
cord-007211-prygoc0q	184	15	detected	detect	VBN
cord-007211-prygoc0q	184	16	at	at	IN
cord-007211-prygoc0q	184	17	the	the	DT
cord-007211-prygoc0q	184	18	cell	cell	NN
cord-007211-prygoc0q	184	19	surface	surface	NN
cord-007211-prygoc0q	184	20	of	of	IN
cord-007211-prygoc0q	184	21	pSRD	psrd	JJ
cord-007211-prygoc0q	184	22	-	-	HYPH
cord-007211-prygoc0q	184	23	HN	hn	NN
cord-007211-prygoc0q	184	24	-	-	HYPH
cord-007211-prygoc0q	184	25	or	or	CC
cord-007211-prygoc0q	184	26	pSRD	pSRD	NNP
cord-007211-prygoc0q	184	27	-	-	HYPH
cord-007211-prygoc0q	184	28	F	f	NN
cord-007211-prygoc0q	184	29	and	and	CC
cord-007211-prygoc0q	184	30	pSRD	psrd	JJ
cord-007211-prygoc0q	184	31	-	-	HYPH
cord-007211-prygoc0q	184	32	HN	hn	NN
cord-007211-prygoc0q	184	33	-	-	HYPH
cord-007211-prygoc0q	184	34	transfected	transfecte	VBN
cord-007211-prygoc0q	184	35	cells	cell	NNS
cord-007211-prygoc0q	184	36	.	.	.
cord-007211-prygoc0q	185	1	On	on	IN
cord-007211-prygoc0q	185	2	the	the	DT
cord-007211-prygoc0q	185	3	other	other	JJ
cord-007211-prygoc0q	185	4	hand	hand	NN
cord-007211-prygoc0q	185	5	,	,	,
cord-007211-prygoc0q	185	6	the	the	DT
cord-007211-prygoc0q	185	7	cell	cell	NN
cord-007211-prygoc0q	185	8	surface	surface	NN
cord-007211-prygoc0q	185	9	expression	expression	NN
cord-007211-prygoc0q	185	10	of	of	IN
cord-007211-prygoc0q	185	11	the	the	DT
cord-007211-prygoc0q	185	12	HN	HN	NNP
cord-007211-prygoc0q	185	13	protein	protein	NN
cord-007211-prygoc0q	185	14	was	be	VBD
cord-007211-prygoc0q	185	15	drastically	drastically	RB
cord-007211-prygoc0q	185	16	reduced	reduce	VBN
cord-007211-prygoc0q	185	17	in	in	IN
cord-007211-prygoc0q	185	18	the	the	DT
cord-007211-prygoc0q	185	19	cells	cell	NNS
cord-007211-prygoc0q	185	20	co	co	VBD
cord-007211-prygoc0q	185	21	-	-	VBN
cord-007211-prygoc0q	185	22	transfected	transfecte	VBN
cord-007211-prygoc0q	185	23	with	with	IN
cord-007211-prygoc0q	185	24	pSRD	psrd	CD
cord-007211-prygoc0q	185	25	-	-	HYPH
cord-007211-prygoc0q	185	26	HN	hn	NN
cord-007211-prygoc0q	185	27	and	and	CC
cord-007211-prygoc0q	185	28	one	one	CD
cord-007211-prygoc0q	185	29	of	of	IN
cord-007211-prygoc0q	185	30	the	the	DT
cord-007211-prygoc0q	185	31	cysteine	cysteine	NN
cord-007211-prygoc0q	185	32	mutant	mutant	JJ
cord-007211-prygoc0q	185	33	F	F	NNP
cord-007211-prygoc0q	185	34	genes	gene	NNS
cord-007211-prygoc0q	185	35	(	(	-LRB-
cord-007211-prygoc0q	185	36	examples	example	NNS
cord-007211-prygoc0q	185	37	shown	show	VBN
cord-007211-prygoc0q	185	38	in	in	IN
cord-007211-prygoc0q	185	39	Fig	Fig	NNP
cord-007211-prygoc0q	185	40	.	.	.
cord-007211-prygoc0q	186	1	5A	5A	NNP
cord-007211-prygoc0q	186	2	panels	panels	NNP
cord-007211-prygoc0q	186	3	d	d	NNP
cord-007211-prygoc0q	186	4	,	,	,
cord-007211-prygoc0q	186	5	e	e	NNP
cord-007211-prygoc0q	186	6	,	,	,
cord-007211-prygoc0q	186	7	and	and	CC
cord-007211-prygoc0q	186	8	f	f	LS
cord-007211-prygoc0q	186	9	)	)	-RRB-
cord-007211-prygoc0q	186	10	.	.	.
cord-007211-prygoc0q	187	1	As	as	IN
cord-007211-prygoc0q	187	2	shown	show	VBN
cord-007211-prygoc0q	187	3	in	in	IN
cord-007211-prygoc0q	187	4	Fig	Fig	NNP
cord-007211-prygoc0q	187	5	.	.	.
cord-007211-prygoc0q	188	1	5B	5B	NNP
cord-007211-prygoc0q	188	2	,	,	,
cord-007211-prygoc0q	188	3	in	in	IN
cord-007211-prygoc0q	188	4	Western	western	JJ
cord-007211-prygoc0q	188	5	blot	blot	NN
cord-007211-prygoc0q	188	6	analysis	analysis	NN
cord-007211-prygoc0q	188	7	,	,	,
cord-007211-prygoc0q	188	8	both	both	DT
cord-007211-prygoc0q	188	9	HN	HN	NNP
cord-007211-prygoc0q	188	10	and	and	CC
cord-007211-prygoc0q	188	11	wild	wild	JJ
cord-007211-prygoc0q	188	12	-	-	HYPH
cord-007211-prygoc0q	188	13	type	type	NN
cord-007211-prygoc0q	188	14	or	or	CC
cord-007211-prygoc0q	188	15	the	the	DT
cord-007211-prygoc0q	188	16	cysteine	cysteine	NN
cord-007211-prygoc0q	188	17	mutant	mutant	NN
cord-007211-prygoc0q	188	18	F	F	NNP
cord-007211-prygoc0q	188	19	proteins	protein	NNS
cord-007211-prygoc0q	188	20	were	be	VBD
cord-007211-prygoc0q	188	21	detected	detect	VBN
cord-007211-prygoc0q	188	22	in	in	IN
cord-007211-prygoc0q	188	23	the	the	DT
cord-007211-prygoc0q	188	24	cells	cell	NNS
cord-007211-prygoc0q	188	25	co	co	VBD
cord-007211-prygoc0q	188	26	-	-	VBN
cord-007211-prygoc0q	188	27	transfected	transfecte	VBN
cord-007211-prygoc0q	188	28	with	with	IN
cord-007211-prygoc0q	188	29	HN	HN	NNP
cord-007211-prygoc0q	188	30	and	and	CC
cord-007211-prygoc0q	188	31	wild	wild	JJ
cord-007211-prygoc0q	188	32	-	-	HYPH
cord-007211-prygoc0q	188	33	type	type	NN
cord-007211-prygoc0q	188	34	or	or	CC
cord-007211-prygoc0q	188	35	cysteine	cysteine	NN
cord-007211-prygoc0q	188	36	mutant	mutant	JJ
cord-007211-prygoc0q	188	37	F	F	NNP
cord-007211-prygoc0q	188	38	genes	gene	NNS
cord-007211-prygoc0q	188	39	(	(	-LRB-
cord-007211-prygoc0q	188	40	lanes	lane	NNS
cord-007211-prygoc0q	188	41	[	[	-LRB-
cord-007211-prygoc0q	188	42	4	4	CD
cord-007211-prygoc0q	188	43	]	]	-RRB-
cord-007211-prygoc0q	188	44	[	[	-LRB-
cord-007211-prygoc0q	188	45	5	5	CD
cord-007211-prygoc0q	188	46	]	]	-RRB-
cord-007211-prygoc0q	188	47	[	[	-LRB-
cord-007211-prygoc0q	188	48	6	6	CD
cord-007211-prygoc0q	188	49	]	]	-RRB-
cord-007211-prygoc0q	188	50	[	[	-LRB-
cord-007211-prygoc0q	188	51	7	7	CD
cord-007211-prygoc0q	188	52	]	]	-RRB-
cord-007211-prygoc0q	189	1	[	[	-LRB-
cord-007211-prygoc0q	189	2	8	8	CD
cord-007211-prygoc0q	189	3	]	]	-RRB-
cord-007211-prygoc0q	189	4	[	[	-LRB-
cord-007211-prygoc0q	189	5	9	9	CD
cord-007211-prygoc0q	189	6	]	]	-RRB-
cord-007211-prygoc0q	189	7	[	[	-LRB-
cord-007211-prygoc0q	189	8	10	10	CD
cord-007211-prygoc0q	189	9	]	]	-RRB-
cord-007211-prygoc0q	189	10	[	[	-LRB-
cord-007211-prygoc0q	189	11	11	11	CD
cord-007211-prygoc0q	189	12	]	]	-RRB-
cord-007211-prygoc0q	190	1	[	[	-LRB-
cord-007211-prygoc0q	190	2	12	12	CD
cord-007211-prygoc0q	190	3	]	]	-RRB-
cord-007211-prygoc0q	190	4	[	[	-LRB-
cord-007211-prygoc0q	190	5	13	13	CD
cord-007211-prygoc0q	190	6	]	]	-RRB-
cord-007211-prygoc0q	191	1	[	[	-LRB-
cord-007211-prygoc0q	191	2	14	14	CD
cord-007211-prygoc0q	191	3	]	]	-RRB-
cord-007211-prygoc0q	191	4	.	.	.
cord-007211-prygoc0q	192	1	The	the	DT
cord-007211-prygoc0q	192	2	expression	expression	NN
cord-007211-prygoc0q	192	3	levels	level	NNS
cord-007211-prygoc0q	192	4	of	of	IN
cord-007211-prygoc0q	192	5	HN	HN	NNP
cord-007211-prygoc0q	192	6	proteins	protein	NNS
cord-007211-prygoc0q	192	7	in	in	IN
cord-007211-prygoc0q	192	8	these	these	DT
cord-007211-prygoc0q	192	9	cells	cell	NNS
cord-007211-prygoc0q	192	10	and	and	CC
cord-007211-prygoc0q	192	11	in	in	IN
cord-007211-prygoc0q	192	12	the	the	DT
cord-007211-prygoc0q	192	13	cells	cell	NNS
cord-007211-prygoc0q	192	14	transfected	transfecte	VBN
cord-007211-prygoc0q	192	15	with	with	IN
cord-007211-prygoc0q	192	16	HN	HN	NNP
cord-007211-prygoc0q	192	17	gene	gene	NN
cord-007211-prygoc0q	192	18	alone	alone	RB
cord-007211-prygoc0q	192	19	were	be	VBD
cord-007211-prygoc0q	192	20	similar	similar	JJ
cord-007211-prygoc0q	192	21	(	(	-LRB-
cord-007211-prygoc0q	192	22	lanes	lane	NNS
cord-007211-prygoc0q	192	23	3	3	CD
cord-007211-prygoc0q	192	24	-	-	SYM
cord-007211-prygoc0q	192	25	14	14	CD
cord-007211-prygoc0q	192	26	)	)	-RRB-
cord-007211-prygoc0q	192	27	.	.	.
cord-007211-prygoc0q	193	1	These	these	DT
cord-007211-prygoc0q	193	2	results	result	NNS
cord-007211-prygoc0q	193	3	indicated	indicate	VBD
cord-007211-prygoc0q	193	4	that	that	IN
cord-007211-prygoc0q	193	5	association	association	NN
cord-007211-prygoc0q	193	6	of	of	IN
cord-007211-prygoc0q	193	7	F	F	NNP
cord-007211-prygoc0q	193	8	and	and	CC
cord-007211-prygoc0q	193	9	HN	HN	NNP
cord-007211-prygoc0q	193	10	proteins	protein	NNS
cord-007211-prygoc0q	193	11	takes	take	VBZ
cord-007211-prygoc0q	193	12	place	place	NN
cord-007211-prygoc0q	193	13	intracellulary	intracellulary	NNP
cord-007211-prygoc0q	193	14	and	and	CC
cord-007211-prygoc0q	193	15	that	that	IN
cord-007211-prygoc0q	193	16	the	the	DT
cord-007211-prygoc0q	193	17	transport	transport	NN
cord-007211-prygoc0q	193	18	deficiency	deficiency	NN
cord-007211-prygoc0q	193	19	of	of	IN
cord-007211-prygoc0q	193	20	the	the	DT
cord-007211-prygoc0q	193	21	cysteine	cysteine	NN
cord-007211-prygoc0q	193	22	mutant	mutant	NN
cord-007211-prygoc0q	193	23	F	F	NNP
cord-007211-prygoc0q	193	24	proteins	protein	NNS
cord-007211-prygoc0q	193	25	affected	affect	VBD
cord-007211-prygoc0q	193	26	the	the	DT
cord-007211-prygoc0q	193	27	intracellular	intracellular	JJ
cord-007211-prygoc0q	193	28	transport	transport	NN
cord-007211-prygoc0q	193	29	of	of	IN
cord-007211-prygoc0q	193	30	the	the	DT
cord-007211-prygoc0q	193	31	HN	HN	NNP
cord-007211-prygoc0q	193	32	protein	protein	NN
cord-007211-prygoc0q	193	33	.	.	.
cord-007211-prygoc0q	194	1	Intracellular	Intracellular	NNP
cord-007211-prygoc0q	194	2	Transport	Transport	NNP
cord-007211-prygoc0q	194	3	of	of	IN
cord-007211-prygoc0q	194	4	F	F	NNP
cord-007211-prygoc0q	194	5	Proteins-	Proteins-	NNP
cord-007211-prygoc0q	195	1	The	the	DT
cord-007211-prygoc0q	195	2	F	F	NNP
cord-007211-prygoc0q	195	3	protein	protein	NN
cord-007211-prygoc0q	195	4	has	have	VBZ
cord-007211-prygoc0q	195	5	three	three	CD
cord-007211-prygoc0q	195	6	iV	iv	CD
cord-007211-prygoc0q	195	7	-	-	HYPH
cord-007211-prygoc0q	195	8	glycosylation	glycosylation	NN
cord-007211-prygoc0q	195	9	motifs	motif	NNS
cord-007211-prygoc0q	195	10	and	and	CC
cord-007211-prygoc0q	195	11	contains	contain	VBZ
cord-007211-prygoc0q	195	12	complextype	complextype	NN
cord-007211-prygoc0q	195	13	sugar	sugar	NN
cord-007211-prygoc0q	195	14	chain(s	chain(s	NNP
cord-007211-prygoc0q	195	15	)	)	-RRB-
cord-007211-prygoc0q	196	1	(	(	-LRB-
cord-007211-prygoc0q	196	2	28	28	CD
cord-007211-prygoc0q	196	3	)	)	-RRB-
cord-007211-prygoc0q	196	4	.	.	.
cord-007211-prygoc0q	197	1	The	the	DT
cord-007211-prygoc0q	197	2	F	F	NNP
cord-007211-prygoc0q	197	3	protein	protein	NN
cord-007211-prygoc0q	197	4	is	be	VBZ
cord-007211-prygoc0q	197	5	synthesized	synthesize	VBN
cord-007211-prygoc0q	197	6	on	on	IN
cord-007211-prygoc0q	197	7	the	the	DT
cord-007211-prygoc0q	197	8	rough	rough	JJ
cord-007211-prygoc0q	197	9	ER	er	NN
cord-007211-prygoc0q	197	10	,	,	,
cord-007211-prygoc0q	197	11	then	then	RB
cord-007211-prygoc0q	197	12	glycosylated	glycosylate	VBN
cord-007211-prygoc0q	197	13	in	in	IN
cord-007211-prygoc0q	197	14	a	a	DT
cord-007211-prygoc0q	197	15	well	well	RB
cord-007211-prygoc0q	197	16	-	-	HYPH
cord-007211-prygoc0q	197	17	defined	define	VBN
cord-007211-prygoc0q	197	18	sequence	sequence	NN
cord-007211-prygoc0q	197	19	in	in	IN
cord-007211-prygoc0q	197	20	the	the	DT
cord-007211-prygoc0q	197	21	ER	ER	NNP
cord-007211-prygoc0q	197	22	and	and	CC
cord-007211-prygoc0q	197	23	Golgi	Golgi	NNP
cord-007211-prygoc0q	197	24	apparatus	apparatus	NN
cord-007211-prygoc0q	197	25	in	in	IN
cord-007211-prygoc0q	197	26	the	the	DT
cord-007211-prygoc0q	197	27	process	process	NN
cord-007211-prygoc0q	197	28	of	of	IN
cord-007211-prygoc0q	197	29	transport	transport	NN
cord-007211-prygoc0q	197	30	to	to	IN
cord-007211-prygoc0q	197	31	the	the	DT
cord-007211-prygoc0q	197	32	cell	cell	NN
cord-007211-prygoc0q	197	33	surface	surface	NN
cord-007211-prygoc0q	197	34	.	.	.
cord-007211-prygoc0q	198	1	High	high	JJ
cord-007211-prygoc0q	198	2	mannose	mannose	NN
cord-007211-prygoc0q	198	3	-	-	HYPH
cord-007211-prygoc0q	198	4	type	type	NN
cord-007211-prygoc0q	198	5	sugar	sugar	NN
cord-007211-prygoc0q	198	6	chains	chain	NNS
cord-007211-prygoc0q	198	7	of	of	IN
cord-007211-prygoc0q	198	8	the	the	DT
cord-007211-prygoc0q	198	9	glycoprotein	glycoprotein	NN
cord-007211-prygoc0q	198	10	in	in	IN
cord-007211-prygoc0q	198	11	the	the	DT
cord-007211-prygoc0q	198	12	ER	ER	NNP
cord-007211-prygoc0q	198	13	and	and	CC
cord-007211-prygoc0q	198	14	the	the	DT
cord-007211-prygoc0q	198	15	cis	cis	NNP
cord-007211-prygoc0q	198	16	-	-	HYPH
cord-007211-prygoc0q	198	17	Golgi	Golgi	NNP
cord-007211-prygoc0q	198	18	compartments	compartment	NNS
cord-007211-prygoc0q	198	19	are	be	VBP
cord-007211-prygoc0q	198	20	sensitive	sensitive	JJ
cord-007211-prygoc0q	198	21	to	to	IN
cord-007211-prygoc0q	198	22	digestion	digestion	NN
cord-007211-prygoc0q	198	23	with	with	IN
cord-007211-prygoc0q	198	24	endoglycosidase	endoglycosidase	NNP
cord-007211-prygoc0q	198	25	H	H	NNP
cord-007211-prygoc0q	198	26	(	(	-LRB-
cord-007211-prygoc0q	198	27	endo	endo	NN
cord-007211-prygoc0q	198	28	H	H	NNP
cord-007211-prygoc0q	198	29	)	)	-RRB-
cord-007211-prygoc0q	198	30	,	,	,
cord-007211-prygoc0q	198	31	while	while	IN
cord-007211-prygoc0q	198	32	sugar	sugar	NN
cord-007211-prygoc0q	198	33	chains	chain	NNS
cord-007211-prygoc0q	198	34	once	once	RB
cord-007211-prygoc0q	198	35	trimmed	trim	VBN
cord-007211-prygoc0q	198	36	and	and	CC
cord-007211-prygoc0q	198	37	further	further	RB
cord-007211-prygoc0q	198	38	processed	process	VBN
cord-007211-prygoc0q	198	39	in	in	IN
cord-007211-prygoc0q	198	40	the	the	DT
cord-007211-prygoc0q	198	41	medial	medial	JJ
cord-007211-prygoc0q	198	42	and	and	CC
cord-007211-prygoc0q	198	43	trans	trans	JJ
cord-007211-prygoc0q	198	44	-	-	JJ
cord-007211-prygoc0q	198	45	Golgi	Golgi	NNP
cord-007211-prygoc0q	198	46	compartments	compartment	NNS
cord-007211-prygoc0q	198	47	are	be	VBP
cord-007211-prygoc0q	198	48	not	not	RB
cord-007211-prygoc0q	198	49	.	.	.
cord-007211-prygoc0q	199	1	We	-PRON-	PRP
cord-007211-prygoc0q	199	2	therefore	therefore	RB
cord-007211-prygoc0q	199	3	determined	determine	VBD
cord-007211-prygoc0q	199	4	the	the	DT
cord-007211-prygoc0q	199	5	critical	critical	JJ
cord-007211-prygoc0q	199	6	state	state	NN
cord-007211-prygoc0q	199	7	in	in	IN
cord-007211-prygoc0q	199	8	the	the	DT
cord-007211-prygoc0q	199	9	transport	transport	NN
cord-007211-prygoc0q	199	10	process	process	NN
cord-007211-prygoc0q	199	11	of	of	IN
cord-007211-prygoc0q	199	12	the	the	DT
cord-007211-prygoc0q	199	13	cysteine	cysteine	NN
cord-007211-prygoc0q	199	14	mutant	mutant	NN
cord-007211-prygoc0q	199	15	F	F	NNP
cord-007211-prygoc0q	199	16	proteins	protein	NNS
cord-007211-prygoc0q	199	17	by	by	IN
cord-007211-prygoc0q	199	18	testing	test	VBG
cord-007211-prygoc0q	199	19	endo	endo	NN
cord-007211-prygoc0q	199	20	H	H	NNP
cord-007211-prygoc0q	199	21	sensitivity	sensitivity	NN
cord-007211-prygoc0q	199	22	of	of	IN
cord-007211-prygoc0q	199	23	the	the	DT
cord-007211-prygoc0q	199	24	sugar	sugar	NN
cord-007211-prygoc0q	199	25	chains	chain	NNS
cord-007211-prygoc0q	199	26	.	.	.
cord-007211-prygoc0q	200	1	To	to	TO
cord-007211-prygoc0q	200	2	follow	follow	VB
cord-007211-prygoc0q	200	3	the	the	DT
cord-007211-prygoc0q	200	4	process	process	NN
cord-007211-prygoc0q	200	5	of	of	IN
cord-007211-prygoc0q	200	6	maturation	maturation	NN
cord-007211-prygoc0q	200	7	of	of	IN
cord-007211-prygoc0q	200	8	glycoproteins	glycoprotein	NNS
cord-007211-prygoc0q	200	9	,	,	,
cord-007211-prygoc0q	200	10	the	the	DT
cord-007211-prygoc0q	200	11	transfected	transfecte	VBN
cord-007211-prygoc0q	200	12	cells	cell	NNS
cord-007211-prygoc0q	200	13	were	be	VBD
cord-007211-prygoc0q	200	14	labeled	label	VBN
cord-007211-prygoc0q	200	15	with	with	IN
cord-007211-prygoc0q	200	16	[	[	-LRB-
cord-007211-prygoc0q	200	17	35	35	CD
cord-007211-prygoc0q	200	18	S]methionine	S]methionine	NNP
cord-007211-prygoc0q	200	19	and	and	CC
cord-007211-prygoc0q	200	20	[	[	-LRB-
cord-007211-prygoc0q	200	21	35	35	CD
cord-007211-prygoc0q	200	22	S	s	NN
cord-007211-prygoc0q	200	23	]	]	-RRB-
cord-007211-prygoc0q	200	24	cysteine	cysteine	NN
cord-007211-prygoc0q	200	25	for	for	IN
cord-007211-prygoc0q	200	26	10	10	CD
cord-007211-prygoc0q	200	27	min	min	NN
cord-007211-prygoc0q	200	28	at	at	IN
cord-007211-prygoc0q	200	29	48	48	CD
cord-007211-prygoc0q	200	30	h	h	NN
cord-007211-prygoc0q	200	31	after	after	IN
cord-007211-prygoc0q	200	32	transfection	transfection	NN
cord-007211-prygoc0q	200	33	.	.	.
cord-007211-prygoc0q	201	1	After	after	IN
cord-007211-prygoc0q	201	2	being	be	VBG
cord-007211-prygoc0q	201	3	chased	chase	VBN
cord-007211-prygoc0q	201	4	for	for	IN
cord-007211-prygoc0q	201	5	3	3	CD
cord-007211-prygoc0q	201	6	h	h	NN
cord-007211-prygoc0q	201	7	,	,	,
cord-007211-prygoc0q	201	8	cells	cell	NNS
cord-007211-prygoc0q	201	9	were	be	VBD
cord-007211-prygoc0q	201	10	lysed	lyse	VBN
cord-007211-prygoc0q	201	11	and	and	CC
cord-007211-prygoc0q	201	12	the	the	DT
cord-007211-prygoc0q	201	13	lysates	lysate	NNS
cord-007211-prygoc0q	201	14	were	be	VBD
cord-007211-prygoc0q	201	15	analyzed	analyze	VBN
cord-007211-prygoc0q	201	16	after	after	IN
cord-007211-prygoc0q	201	17	immunoprecipitation	immunoprecipitation	NN
cord-007211-prygoc0q	201	18	with	with	IN
cord-007211-prygoc0q	201	19	anti	anti	JJ
cord-007211-prygoc0q	201	20	-	-	NNP
cord-007211-prygoc0q	201	21	F	F	NNP
cord-007211-prygoc0q	201	22	followed	follow	VBN
cord-007211-prygoc0q	201	23	by	by	IN
cord-007211-prygoc0q	201	24	endo	endo	NN
cord-007211-prygoc0q	201	25	H	H	NNP
cord-007211-prygoc0q	201	26	digestion	digestion	NN
cord-007211-prygoc0q	201	27	,	,	,
cord-007211-prygoc0q	201	28	as	as	IN
cord-007211-prygoc0q	201	29	described	describe	VBN
cord-007211-prygoc0q	201	30	in	in	IN
cord-007211-prygoc0q	201	31	"	"	``
cord-007211-prygoc0q	201	32	MATERIALS	materials	NN
cord-007211-prygoc0q	201	33	AND	and	CC
cord-007211-prygoc0q	201	34	METHODS	method	NNS
cord-007211-prygoc0q	201	35	.	.	.
cord-007211-prygoc0q	201	36	"	"	''
cord-007211-prygoc0q	202	1	As	as	IN
cord-007211-prygoc0q	202	2	shown	show	VBN
cord-007211-prygoc0q	202	3	in	in	IN
cord-007211-prygoc0q	202	4	pSRD	psrd	CD
cord-007211-prygoc0q	202	5	-	-	HYPH
cord-007211-prygoc0q	202	6	FClS	fcls	NN
cord-007211-prygoc0q	202	7	-	-	HYPH
cord-007211-prygoc0q	202	8	transfected	transfecte	VBN
cord-007211-prygoc0q	202	9	cells	cell	NNS
cord-007211-prygoc0q	202	10	was	be	VBD
cord-007211-prygoc0q	202	11	partially	partially	RB
cord-007211-prygoc0q	202	12	glycosylated	glycosylate	VBN
cord-007211-prygoc0q	202	13	F	F	NNP
cord-007211-prygoc0q	202	14	protein	protein	NN
cord-007211-prygoc0q	202	15	modified	modify	VBN
cord-007211-prygoc0q	202	16	at	at	IN
cord-007211-prygoc0q	202	17	a	a	DT
cord-007211-prygoc0q	202	18	novel	novel	JJ
cord-007211-prygoc0q	202	19	glycosylation	glycosylation	NN
cord-007211-prygoc0q	202	20	motif	motif	NN
cord-007211-prygoc0q	202	21	generated	generate	VBN
cord-007211-prygoc0q	202	22	by	by	IN
cord-007211-prygoc0q	202	23	the	the	DT
cord-007211-prygoc0q	202	24	mutagenesis	mutagenesis	NN
cord-007211-prygoc0q	202	25	.	.	.
cord-007211-prygoc0q	203	1	Because	because	IN
cord-007211-prygoc0q	203	2	the	the	DT
cord-007211-prygoc0q	203	3	antiserum	antiserum	NN
cord-007211-prygoc0q	203	4	used	use	VBN
cord-007211-prygoc0q	203	5	in	in	IN
cord-007211-prygoc0q	203	6	this	this	DT
cord-007211-prygoc0q	203	7	study	study	NN
cord-007211-prygoc0q	203	8	reacts	react	VBZ
cord-007211-prygoc0q	203	9	efficiently	efficiently	RB
cord-007211-prygoc0q	203	10	with	with	IN
cord-007211-prygoc0q	203	11	immature	immature	JJ
cord-007211-prygoc0q	203	12	F	F	NNP
cord-007211-prygoc0q	203	13	protein	protein	NN
cord-007211-prygoc0q	203	14	,	,	,
cord-007211-prygoc0q	203	15	relatively	relatively	RB
cord-007211-prygoc0q	203	16	large	large	JJ
cord-007211-prygoc0q	203	17	amounts	amount	NNS
cord-007211-prygoc0q	203	18	of	of	IN
cord-007211-prygoc0q	203	19	endo	endo	NN
cord-007211-prygoc0q	203	20	H	h	NN
cord-007211-prygoc0q	203	21	-	-	HYPH
cord-007211-prygoc0q	203	22	sensitive	sensitive	JJ
cord-007211-prygoc0q	203	23	species	specie	NNS
cord-007211-prygoc0q	203	24	of	of	IN
cord-007211-prygoc0q	203	25	F	f	NN
cord-007211-prygoc0q	203	26	proteins	protein	NNS
cord-007211-prygoc0q	203	27	were	be	VBD
cord-007211-prygoc0q	203	28	detected	detect	VBN
cord-007211-prygoc0q	203	29	even	even	RB
cord-007211-prygoc0q	203	30	in	in	IN
cord-007211-prygoc0q	203	31	the	the	DT
cord-007211-prygoc0q	203	32	cells	cell	NNS
cord-007211-prygoc0q	203	33	expressing	express	VBG
cord-007211-prygoc0q	203	34	wild	wild	JJ
cord-007211-prygoc0q	203	35	-	-	HYPH
cord-007211-prygoc0q	203	36	type	type	NN
cord-007211-prygoc0q	203	37	F	F	NNP
cord-007211-prygoc0q	203	38	protein	protein	NN
cord-007211-prygoc0q	203	39	.	.	.
cord-007211-prygoc0q	204	1	These	these	DT
cord-007211-prygoc0q	204	2	data	datum	NNS
cord-007211-prygoc0q	204	3	showed	show	VBD
cord-007211-prygoc0q	204	4	that	that	IN
cord-007211-prygoc0q	204	5	cysteine	cysteine	NN
cord-007211-prygoc0q	204	6	mutant	mutant	NN
cord-007211-prygoc0q	204	7	F	F	NNP
cord-007211-prygoc0q	204	8	proteins	protein	NNS
cord-007211-prygoc0q	204	9	were	be	VBD
cord-007211-prygoc0q	204	10	trapped	trap	VBN
cord-007211-prygoc0q	204	11	in	in	IN
cord-007211-prygoc0q	204	12	the	the	DT
cord-007211-prygoc0q	204	13	ER	ER	NNP
cord-007211-prygoc0q	204	14	or	or	CC
cord-007211-prygoc0q	204	15	the	the	DT
cord-007211-prygoc0q	204	16	cis	cis	NNP
cord-007211-prygoc0q	204	17	-	-	HYPH
cord-007211-prygoc0q	204	18	Golgi	Golgi	NNP
cord-007211-prygoc0q	204	19	region	region	NN
cord-007211-prygoc0q	204	20	,	,	,
cord-007211-prygoc0q	204	21	which	which	WDT
cord-007211-prygoc0q	204	22	prevented	prevent	VBD
cord-007211-prygoc0q	204	23	their	-PRON-	PRP$
cord-007211-prygoc0q	204	24	transport	transport	NN
cord-007211-prygoc0q	204	25	to	to	IN
cord-007211-prygoc0q	204	26	the	the	DT
cord-007211-prygoc0q	204	27	cell	cell	NN
cord-007211-prygoc0q	204	28	surface	surface	NN
cord-007211-prygoc0q	204	29	.	.	.
cord-007211-prygoc0q	205	1	We	-PRON-	PRP
cord-007211-prygoc0q	205	2	used	use	VBD
cord-007211-prygoc0q	205	3	mutagenesis	mutagenesis	NN
cord-007211-prygoc0q	205	4	of	of	IN
cord-007211-prygoc0q	205	5	the	the	DT
cord-007211-prygoc0q	205	6	cysteine	cysteine	NN
cord-007211-prygoc0q	205	7	residue	residue	NN
cord-007211-prygoc0q	205	8	of	of	IN
cord-007211-prygoc0q	205	9	the	the	DT
cord-007211-prygoc0q	205	10	F	F	NNP
cord-007211-prygoc0q	205	11	protein	protein	NN
cord-007211-prygoc0q	205	12	to	to	TO
cord-007211-prygoc0q	205	13	examine	examine	VB
cord-007211-prygoc0q	205	14	the	the	DT
cord-007211-prygoc0q	205	15	effect	effect	NN
cord-007211-prygoc0q	205	16	of	of	IN
cord-007211-prygoc0q	205	17	the	the	DT
cord-007211-prygoc0q	205	18	disruption	disruption	NN
cord-007211-prygoc0q	205	19	and	and	CC
cord-007211-prygoc0q	205	20	rearrangement	rearrangement	NN
cord-007211-prygoc0q	205	21	of	of	IN
cord-007211-prygoc0q	205	22	the	the	DT
cord-007211-prygoc0q	205	23	disulfide	disulfide	JJ
cord-007211-prygoc0q	205	24	bonds	bond	NNS
cord-007211-prygoc0q	205	25	on	on	IN
cord-007211-prygoc0q	205	26	the	the	DT
cord-007211-prygoc0q	205	27	folding	folding	NN
cord-007211-prygoc0q	205	28	of	of	IN
cord-007211-prygoc0q	205	29	the	the	DT
cord-007211-prygoc0q	205	30	F	F	NNP
cord-007211-prygoc0q	205	31	protein	protein	NN
cord-007211-prygoc0q	205	32	and	and	CC
cord-007211-prygoc0q	205	33	its	-PRON-	PRP$
cord-007211-prygoc0q	205	34	transport	transport	NN
cord-007211-prygoc0q	205	35	to	to	IN
cord-007211-prygoc0q	205	36	the	the	DT
cord-007211-prygoc0q	205	37	cell	cell	NN
cord-007211-prygoc0q	205	38	surface	surface	NN
cord-007211-prygoc0q	205	39	.	.	.
cord-007211-prygoc0q	206	1	We	-PRON-	PRP
cord-007211-prygoc0q	206	2	found	find	VBD
cord-007211-prygoc0q	206	3	that	that	IN
cord-007211-prygoc0q	206	4	all	all	DT
cord-007211-prygoc0q	206	5	cysteine	cysteine	NN
cord-007211-prygoc0q	206	6	mutant	mutant	JJ
cord-007211-prygoc0q	206	7	F	F	NNP
cord-007211-prygoc0q	206	8	proteins	protein	NNS
cord-007211-prygoc0q	206	9	were	be	VBD
cord-007211-prygoc0q	206	10	efficiently	efficiently	RB
cord-007211-prygoc0q	206	11	expressed	express	VBN
cord-007211-prygoc0q	206	12	in	in	IN
cord-007211-prygoc0q	206	13	COS	COS	NNP
cord-007211-prygoc0q	206	14	cells	cell	NNS
cord-007211-prygoc0q	206	15	(	(	-LRB-
cord-007211-prygoc0q	206	16	Fig	Fig	NNP
cord-007211-prygoc0q	206	17	.	.	.
cord-007211-prygoc0q	206	18	2A	2a	CD
cord-007211-prygoc0q	206	19	)	)	-RRB-
cord-007211-prygoc0q	206	20	,	,	,
cord-007211-prygoc0q	206	21	but	but	CC
cord-007211-prygoc0q	206	22	failed	fail	VBD
cord-007211-prygoc0q	206	23	to	to	TO
cord-007211-prygoc0q	206	24	fold	fold	VB
cord-007211-prygoc0q	206	25	into	into	IN
cord-007211-prygoc0q	206	26	the	the	DT
cord-007211-prygoc0q	206	27	compact	compact	JJ
cord-007211-prygoc0q	206	28	form	form	NN
cord-007211-prygoc0q	206	29	and	and	CC
cord-007211-prygoc0q	206	30	to	to	TO
cord-007211-prygoc0q	206	31	achieve	achieve	VB
cord-007211-prygoc0q	206	32	proper	proper	JJ
cord-007211-prygoc0q	206	33	conformation	conformation	NN
cord-007211-prygoc0q	206	34	(	(	-LRB-
cord-007211-prygoc0q	206	35	Fig	fig	NN
cord-007211-prygoc0q	206	36	.	.	.
cord-007211-prygoc0q	206	37	3	3	CD
cord-007211-prygoc0q	206	38	)	)	-RRB-
cord-007211-prygoc0q	206	39	.	.	.
cord-007211-prygoc0q	207	1	They	-PRON-	PRP
cord-007211-prygoc0q	207	2	were	be	VBD
cord-007211-prygoc0q	207	3	retained	retain	VBN
cord-007211-prygoc0q	207	4	in	in	IN
cord-007211-prygoc0q	207	5	the	the	DT
cord-007211-prygoc0q	207	6	ER	ER	NNP
cord-007211-prygoc0q	207	7	to	to	IN
cord-007211-prygoc0q	207	8	cis	cis	NNP
cord-007211-prygoc0q	207	9	-	-	HYPH
cord-007211-prygoc0q	207	10	Golgi	Golgi	NNP
cord-007211-prygoc0q	207	11	compartments	compartment	NNS
cord-007211-prygoc0q	207	12	(	(	-LRB-
cord-007211-prygoc0q	207	13	Fig	fig	NN
cord-007211-prygoc0q	207	14	.	.	.
cord-007211-prygoc0q	207	15	6	6	CD
cord-007211-prygoc0q	207	16	)	)	-RRB-
cord-007211-prygoc0q	207	17	,	,	,
cord-007211-prygoc0q	207	18	formed	form	VBN
cord-007211-prygoc0q	207	19	aggregates	aggregate	NNS
cord-007211-prygoc0q	207	20	(	(	-LRB-
cord-007211-prygoc0q	207	21	Fig	Fig	NNP
cord-007211-prygoc0q	207	22	.	.	.
cord-007211-prygoc0q	207	23	2B	2B	NNP
cord-007211-prygoc0q	207	24	)	)	-RRB-
cord-007211-prygoc0q	207	25	,	,	,
cord-007211-prygoc0q	207	26	and	and	CC
cord-007211-prygoc0q	207	27	failed	fail	VBD
cord-007211-prygoc0q	207	28	to	to	TO
cord-007211-prygoc0q	207	29	be	be	VB
cord-007211-prygoc0q	207	30	transported	transport	VBN
cord-007211-prygoc0q	207	31	to	to	IN
cord-007211-prygoc0q	207	32	the	the	DT
cord-007211-prygoc0q	207	33	cell	cell	NN
cord-007211-prygoc0q	207	34	surface	surface	NN
cord-007211-prygoc0q	207	35	(	(	-LRB-
cord-007211-prygoc0q	207	36	Fig	Fig	NNP
cord-007211-prygoc0q	207	37	.	.	.
cord-007211-prygoc0q	207	38	4	4	CD
cord-007211-prygoc0q	207	39	)	)	-RRB-
cord-007211-prygoc0q	207	40	.	.	.
cord-007211-prygoc0q	208	1	This	this	DT
cord-007211-prygoc0q	208	2	indicated	indicate	VBD
cord-007211-prygoc0q	208	3	that	that	IN
cord-007211-prygoc0q	208	4	disulfide	disulfide	NN
cord-007211-prygoc0q	208	5	bond	bond	NN
cord-007211-prygoc0q	208	6	formation	formation	NN
cord-007211-prygoc0q	208	7	involving	involve	VBG
cord-007211-prygoc0q	208	8	all	all	DT
cord-007211-prygoc0q	208	9	cysteine	cysteine	NN
cord-007211-prygoc0q	208	10	residues	residue	NNS
cord-007211-prygoc0q	208	11	is	be	VBZ
cord-007211-prygoc0q	208	12	required	require	VBN
cord-007211-prygoc0q	208	13	for	for	IN
cord-007211-prygoc0q	208	14	the	the	DT
cord-007211-prygoc0q	208	15	F	F	NNP
cord-007211-prygoc0q	208	16	protein	protein	NN
cord-007211-prygoc0q	208	17	to	to	TO
cord-007211-prygoc0q	208	18	exit	exit	VB
cord-007211-prygoc0q	208	19	the	the	DT
cord-007211-prygoc0q	208	20	ER	er	NN
cord-007211-prygoc0q	208	21	.	.	.
cord-007211-prygoc0q	209	1	Although	although	IN
cord-007211-prygoc0q	209	2	our	-PRON-	PRP$
cord-007211-prygoc0q	209	3	cysteine	cysteine	NN
cord-007211-prygoc0q	209	4	mutant	mutant	NN
cord-007211-prygoc0q	209	5	F	F	NNP
cord-007211-prygoc0q	209	6	proteins	protein	NNS
cord-007211-prygoc0q	209	7	were	be	VBD
cord-007211-prygoc0q	209	8	glycosylated	glycosylate	VBN
cord-007211-prygoc0q	209	9	,	,	,
cord-007211-prygoc0q	209	10	none	none	NN
cord-007211-prygoc0q	209	11	of	of	IN
cord-007211-prygoc0q	209	12	them	-PRON-	PRP
cord-007211-prygoc0q	209	13	acquired	acquire	VBD
cord-007211-prygoc0q	209	14	native	native	JJ
cord-007211-prygoc0q	209	15	structure	structure	NN
cord-007211-prygoc0q	209	16	.	.	.
cord-007211-prygoc0q	210	1	Assignment	assignment	NN
cord-007211-prygoc0q	210	2	of	of	IN
cord-007211-prygoc0q	210	3	disulfide	disulfide	JJ
cord-007211-prygoc0q	210	4	bonds	bond	NNS
cord-007211-prygoc0q	210	5	of	of	IN
cord-007211-prygoc0q	210	6	the	the	DT
cord-007211-prygoc0q	210	7	F	F	NNP
cord-007211-prygoc0q	210	8	protein	protein	NN
cord-007211-prygoc0q	210	9	has	have	VBZ
cord-007211-prygoc0q	210	10	been	be	VBN
cord-007211-prygoc0q	210	11	reported	report	VBN
cord-007211-prygoc0q	210	12	(	(	-LRB-
cord-007211-prygoc0q	210	13	15	15	CD
cord-007211-prygoc0q	210	14	)	)	-RRB-
cord-007211-prygoc0q	210	15	;	;	:
cord-007211-prygoc0q	210	16	thus	thus	RB
cord-007211-prygoc0q	210	17	,	,	,
cord-007211-prygoc0q	210	18	we	-PRON-	PRP
cord-007211-prygoc0q	210	19	expected	expect	VBD
cord-007211-prygoc0q	210	20	that	that	IN
cord-007211-prygoc0q	210	21	we	-PRON-	PRP
cord-007211-prygoc0q	210	22	would	would	MD
cord-007211-prygoc0q	210	23	be	be	VB
cord-007211-prygoc0q	210	24	able	able	JJ
cord-007211-prygoc0q	210	25	to	to	TO
cord-007211-prygoc0q	210	26	detect	detect	VB
cord-007211-prygoc0q	210	27	some	some	DT
cord-007211-prygoc0q	210	28	specific	specific	JJ
cord-007211-prygoc0q	210	29	folding	folding	NN
cord-007211-prygoc0q	210	30	intermediates	intermediate	NNS
cord-007211-prygoc0q	210	31	corresponding	correspond	VBG
cord-007211-prygoc0q	210	32	to	to	IN
cord-007211-prygoc0q	210	33	the	the	DT
cord-007211-prygoc0q	210	34	lack	lack	NN
cord-007211-prygoc0q	210	35	of	of	IN
cord-007211-prygoc0q	210	36	individual	individual	JJ
cord-007211-prygoc0q	210	37	disulfide	disulfide	NN
cord-007211-prygoc0q	210	38	bonds	bond	NNS
cord-007211-prygoc0q	210	39	by	by	IN
cord-007211-prygoc0q	210	40	analyzing	analyze	VBG
cord-007211-prygoc0q	210	41	individual	individual	JJ
cord-007211-prygoc0q	210	42	cysteine	cysteine	NN
cord-007211-prygoc0q	210	43	mutant	mutant	NN
cord-007211-prygoc0q	210	44	F	F	NNP
cord-007211-prygoc0q	210	45	proteins	protein	NNS
cord-007211-prygoc0q	210	46	under	under	IN
cord-007211-prygoc0q	210	47	nonreducing	nonreducing	NN
cord-007211-prygoc0q	210	48	conditions	condition	NNS
cord-007211-prygoc0q	210	49	.	.	.
cord-007211-prygoc0q	211	1	When	when	WRB
cord-007211-prygoc0q	211	2	mutations	mutation	NNS
cord-007211-prygoc0q	211	3	are	be	VBP
cord-007211-prygoc0q	211	4	made	make	VBN
cord-007211-prygoc0q	211	5	in	in	IN
cord-007211-prygoc0q	211	6	either	either	DT
cord-007211-prygoc0q	211	7	one	one	CD
cord-007211-prygoc0q	211	8	of	of	IN
cord-007211-prygoc0q	211	9	a	a	DT
cord-007211-prygoc0q	211	10	pair	pair	NN
cord-007211-prygoc0q	211	11	of	of	IN
cord-007211-prygoc0q	211	12	cysteines	cysteine	NNS
cord-007211-prygoc0q	211	13	that	that	WDT
cord-007211-prygoc0q	211	14	are	be	VBP
cord-007211-prygoc0q	211	15	normally	normally	RB
cord-007211-prygoc0q	211	16	linked	link	VBN
cord-007211-prygoc0q	211	17	in	in	IN
cord-007211-prygoc0q	211	18	a	a	DT
cord-007211-prygoc0q	211	19	mature	mature	JJ
cord-007211-prygoc0q	211	20	structure	structure	NN
cord-007211-prygoc0q	211	21	,	,	,
cord-007211-prygoc0q	211	22	the	the	DT
cord-007211-prygoc0q	211	23	phenotype	phenotype	NN
cord-007211-prygoc0q	211	24	of	of	IN
cord-007211-prygoc0q	211	25	the	the	DT
cord-007211-prygoc0q	211	26	mutant	mutant	JJ
cord-007211-prygoc0q	211	27	proteins	protein	NNS
cord-007211-prygoc0q	211	28	is	be	VBZ
cord-007211-prygoc0q	211	29	often	often	RB
cord-007211-prygoc0q	211	30	similar	similar	JJ
cord-007211-prygoc0q	211	31	.	.	.
cord-007211-prygoc0q	212	1	In	in	IN
cord-007211-prygoc0q	212	2	our	-PRON-	PRP$
cord-007211-prygoc0q	212	3	study	study	NN
cord-007211-prygoc0q	212	4	,	,	,
cord-007211-prygoc0q	212	5	two	two	CD
cord-007211-prygoc0q	212	6	species	specie	NNS
cord-007211-prygoc0q	212	7	of	of	IN
cord-007211-prygoc0q	212	8	F	f	NN
cord-007211-prygoc0q	212	9	proteins	protein	NNS
cord-007211-prygoc0q	212	10	were	be	VBD
cord-007211-prygoc0q	212	11	detected	detect	VBN
cord-007211-prygoc0q	212	12	in	in	IN
cord-007211-prygoc0q	212	13	the	the	DT
cord-007211-prygoc0q	212	14	cysteine	cysteine	NN
cord-007211-prygoc0q	212	15	mutant	mutant	NN
cord-007211-prygoc0q	212	16	F	F	NNP
cord-007211-prygoc0q	212	17	proteins	protein	NNS
cord-007211-prygoc0q	212	18	under	under	IN
cord-007211-prygoc0q	212	19	nonreducing	nonreducing	NN
cord-007211-prygoc0q	212	20	conditions	condition	NNS
cord-007211-prygoc0q	212	21	:	:	:
cord-007211-prygoc0q	212	22	folded	fold	VBD
cord-007211-prygoc0q	212	23	F	F	NNP
cord-007211-prygoc0q	212	24	protein	protein	NN
cord-007211-prygoc0q	212	25	and	and	CC
cord-007211-prygoc0q	212	26	unfolded	unfold	VBD
cord-007211-prygoc0q	212	27	F	F	NNP
cord-007211-prygoc0q	212	28	protein	protein	NN
cord-007211-prygoc0q	212	29	.	.	.
cord-007211-prygoc0q	213	1	This	this	DT
cord-007211-prygoc0q	213	2	indicated	indicate	VBD
cord-007211-prygoc0q	213	3	that	that	IN
cord-007211-prygoc0q	213	4	formation	formation	NN
cord-007211-prygoc0q	213	5	of	of	IN
cord-007211-prygoc0q	213	6	incorrect	incorrect	JJ
cord-007211-prygoc0q	213	7	disulfide	disulfide	JJ
cord-007211-prygoc0q	213	8	bonds	bond	NNS
cord-007211-prygoc0q	213	9	led	lead	VBD
cord-007211-prygoc0q	213	10	to	to	IN
cord-007211-prygoc0q	213	11	rapid	rapid	JJ
cord-007211-prygoc0q	213	12	unfolding	unfolding	NN
cord-007211-prygoc0q	213	13	or	or	CC
cord-007211-prygoc0q	213	14	rearrangement	rearrangement	NN
cord-007211-prygoc0q	213	15	of	of	IN
cord-007211-prygoc0q	213	16	disulfide	disulfide	JJ
cord-007211-prygoc0q	213	17	bonds	bond	NNS
cord-007211-prygoc0q	213	18	.	.	.
cord-007211-prygoc0q	214	1	As	as	IN
cord-007211-prygoc0q	214	2	shown	show	VBN
cord-007211-prygoc0q	214	3	in	in	IN
cord-007211-prygoc0q	214	4	Fig	Fig	NNP
cord-007211-prygoc0q	214	5	.	.	.
cord-007211-prygoc0q	215	1	3	3	LS
cord-007211-prygoc0q	215	2	,	,	,
cord-007211-prygoc0q	215	3	almost	almost	RB
cord-007211-prygoc0q	215	4	all	all	DT
cord-007211-prygoc0q	215	5	cysteine	cysteine	NN
cord-007211-prygoc0q	215	6	mutants	mutant	NNS
cord-007211-prygoc0q	215	7	exhibited	exhibit	VBD
cord-007211-prygoc0q	215	8	a	a	DT
cord-007211-prygoc0q	215	9	drastic	drastic	JJ
cord-007211-prygoc0q	215	10	decrease	decrease	NN
cord-007211-prygoc0q	215	11	in	in	IN
cord-007211-prygoc0q	215	12	reactivity	reactivity	NN
cord-007211-prygoc0q	215	13	to	to	IN
cord-007211-prygoc0q	215	14	the	the	DT
cord-007211-prygoc0q	215	15	mature	mature	JJ
cord-007211-prygoc0q	215	16	conformation	conformation	NN
cord-007211-prygoc0q	215	17	specific	specific	JJ
cord-007211-prygoc0q	215	18	MAb	MAb	NNP
cord-007211-prygoc0q	215	19	f-49	f-49	NNP
cord-007211-prygoc0q	215	20	.	.	.
cord-007211-prygoc0q	216	1	This	this	DT
cord-007211-prygoc0q	216	2	result	result	NN
cord-007211-prygoc0q	216	3	showed	show	VBD
cord-007211-prygoc0q	216	4	that	that	IN
cord-007211-prygoc0q	216	5	cysteine	cysteine	NN
cord-007211-prygoc0q	216	6	residues	residue	NNS
cord-007211-prygoc0q	216	7	contribute	contribute	VBP
cord-007211-prygoc0q	216	8	to	to	IN
cord-007211-prygoc0q	216	9	the	the	DT
cord-007211-prygoc0q	216	10	whole	whole	JJ
cord-007211-prygoc0q	216	11	protein	protein	NN
cord-007211-prygoc0q	216	12	structure	structure	NN
cord-007211-prygoc0q	216	13	through	through	IN
cord-007211-prygoc0q	216	14	disulfide	disulfide	JJ
cord-007211-prygoc0q	216	15	bridging	bridging	NN
cord-007211-prygoc0q	216	16	,	,	,
cord-007211-prygoc0q	216	17	even	even	RB
cord-007211-prygoc0q	216	18	though	though	IN
cord-007211-prygoc0q	216	19	they	-PRON-	PRP
cord-007211-prygoc0q	216	20	are	be	VBP
cord-007211-prygoc0q	216	21	fairly	fairly	RB
cord-007211-prygoc0q	216	22	distant	distant	JJ
cord-007211-prygoc0q	216	23	from	from	IN
cord-007211-prygoc0q	216	24	each	each	DT
cord-007211-prygoc0q	216	25	other	other	JJ
cord-007211-prygoc0q	216	26	in	in	IN
cord-007211-prygoc0q	216	27	the	the	DT
cord-007211-prygoc0q	216	28	primary	primary	JJ
cord-007211-prygoc0q	216	29	amino	amino	NN
cord-007211-prygoc0q	216	30	acid	acid	NN
cord-007211-prygoc0q	216	31	sequence	sequence	NN
cord-007211-prygoc0q	216	32	.	.	.
cord-007211-prygoc0q	217	1	MAb	MAb	NNS
cord-007211-prygoc0q	217	2	f-921	f-921	NNS
cord-007211-prygoc0q	217	3	could	could	MD
cord-007211-prygoc0q	217	4	not	not	RB
cord-007211-prygoc0q	217	5	immunoprecipitate	immunoprecipitate	VB
cord-007211-prygoc0q	217	6	FC5S	FC5S	NNP
cord-007211-prygoc0q	217	7	and	and	CC
cord-007211-prygoc0q	217	8	FC6S	FC6S	NNP
cord-007211-prygoc0q	217	9	proteins	protein	NNS
cord-007211-prygoc0q	217	10	but	but	CC
cord-007211-prygoc0q	217	11	could	could	MD
cord-007211-prygoc0q	217	12	immunoprecipitate	immunoprecipitate	VB
cord-007211-prygoc0q	217	13	the	the	DT
cord-007211-prygoc0q	217	14	rest	rest	NN
cord-007211-prygoc0q	217	15	of	of	IN
cord-007211-prygoc0q	217	16	the	the	DT
cord-007211-prygoc0q	217	17	mutants	mutant	NNS
cord-007211-prygoc0q	217	18	.	.	.
cord-007211-prygoc0q	218	1	This	this	DT
cord-007211-prygoc0q	218	2	implied	imply	VBD
cord-007211-prygoc0q	218	3	that	that	IN
cord-007211-prygoc0q	218	4	the	the	DT
cord-007211-prygoc0q	218	5	epitope	epitope	NN
cord-007211-prygoc0q	218	6	of	of	IN
cord-007211-prygoc0q	218	7	f-921	f-921	NNS
cord-007211-prygoc0q	218	8	is	be	VBZ
cord-007211-prygoc0q	218	9	present	present	JJ
cord-007211-prygoc0q	218	10	in	in	IN
cord-007211-prygoc0q	218	11	the	the	DT
cord-007211-prygoc0q	218	12	loop	loop	NN
cord-007211-prygoc0q	218	13	formed	form	VBN
cord-007211-prygoc0q	218	14	by	by	IN
cord-007211-prygoc0q	218	15	the	the	DT
cord-007211-prygoc0q	218	16	C5	C5	NNP
cord-007211-prygoc0q	218	17	and	and	CC
cord-007211-prygoc0q	218	18	C6	C6	NNP
cord-007211-prygoc0q	218	19	disulfide	disulfide	NN
cord-007211-prygoc0q	218	20	bond	bond	NN
cord-007211-prygoc0q	218	21	,	,	,
cord-007211-prygoc0q	218	22	and	and	CC
cord-007211-prygoc0q	218	23	the	the	DT
cord-007211-prygoc0q	218	24	antigenic	antigenic	JJ
cord-007211-prygoc0q	218	25	site	site	NN
cord-007211-prygoc0q	218	26	is	be	VBZ
cord-007211-prygoc0q	218	27	exposed	expose	VBN
cord-007211-prygoc0q	218	28	when	when	WRB
cord-007211-prygoc0q	218	29	the	the	DT
cord-007211-prygoc0q	218	30	F	F	NNP
cord-007211-prygoc0q	218	31	protein	protein	NN
cord-007211-prygoc0q	218	32	is	be	VBZ
cord-007211-prygoc0q	218	33	inside	inside	IN
cord-007211-prygoc0q	218	34	the	the	DT
cord-007211-prygoc0q	218	35	cells	cell	NNS
cord-007211-prygoc0q	218	36	,	,	,
cord-007211-prygoc0q	218	37	but	but	CC
cord-007211-prygoc0q	218	38	buried	bury	VBN
cord-007211-prygoc0q	218	39	when	when	WRB
cord-007211-prygoc0q	218	40	the	the	DT
cord-007211-prygoc0q	218	41	F	F	NNP
cord-007211-prygoc0q	218	42	protein	protein	NN
cord-007211-prygoc0q	218	43	is	be	VBZ
cord-007211-prygoc0q	218	44	expressed	express	VBN
cord-007211-prygoc0q	218	45	on	on	IN
cord-007211-prygoc0q	218	46	the	the	DT
cord-007211-prygoc0q	218	47	cell	cell	NN
cord-007211-prygoc0q	218	48	surface	surface	NN
cord-007211-prygoc0q	218	49	.	.	.
cord-007211-prygoc0q	219	1	FC2S	FC2S	NNP
cord-007211-prygoc0q	219	2	showed	show	VBD
cord-007211-prygoc0q	219	3	only	only	RB
cord-007211-prygoc0q	219	4	slight	slight	JJ
cord-007211-prygoc0q	219	5	reactivity	reactivity	NN
cord-007211-prygoc0q	219	6	to	to	IN
cord-007211-prygoc0q	219	7	MAb	MAb	NNS
cord-007211-prygoc0q	219	8	f-236	f-236	NN
cord-007211-prygoc0q	219	9	.	.	.
cord-007211-prygoc0q	220	1	These	these	DT
cord-007211-prygoc0q	220	2	data	datum	NNS
cord-007211-prygoc0q	220	3	indicated	indicate	VBD
cord-007211-prygoc0q	220	4	that	that	IN
cord-007211-prygoc0q	220	5	f-236	f-236	NN
cord-007211-prygoc0q	220	6	and	and	CC
cord-007211-prygoc0q	220	7	f-921	f-921	NNS
cord-007211-prygoc0q	220	8	may	may	MD
cord-007211-prygoc0q	220	9	recognize	recognize	VB
cord-007211-prygoc0q	220	10	cysteine	cysteine	NN
cord-007211-prygoc0q	220	11	-	-	HYPH
cord-007211-prygoc0q	220	12	rich	rich	JJ
cord-007211-prygoc0q	220	13	domains	domain	NNS
cord-007211-prygoc0q	220	14	and	and	CC
cord-007211-prygoc0q	220	15	supported	support	VBD
cord-007211-prygoc0q	220	16	the	the	DT
cord-007211-prygoc0q	220	17	idea	idea	NN
cord-007211-prygoc0q	220	18	that	that	IN
cord-007211-prygoc0q	220	19	the	the	DT
cord-007211-prygoc0q	220	20	cysteine	cysteine	NN
cord-007211-prygoc0q	220	21	-	-	HYPH
cord-007211-prygoc0q	220	22	rich	rich	JJ
cord-007211-prygoc0q	220	23	domain	domain	NN
cord-007211-prygoc0q	220	24	in	in	IN
cord-007211-prygoc0q	220	25	the	the	DT
cord-007211-prygoc0q	220	26	bunched	bunch	VBN
cord-007211-prygoc0q	220	27	structure	structure	NN
cord-007211-prygoc0q	220	28	of	of	IN
cord-007211-prygoc0q	220	29	paramyxovirus	paramyxovirus	NNP
cord-007211-prygoc0q	220	30	F	F	NNP
cord-007211-prygoc0q	220	31	proteins	protein	NNS
cord-007211-prygoc0q	220	32	is	be	VBZ
cord-007211-prygoc0q	220	33	recognized	recognize	VBN
cord-007211-prygoc0q	220	34	by	by	IN
cord-007211-prygoc0q	220	35	MAbs	MAbs	NNP
cord-007211-prygoc0q	220	36	(	(	-LRB-
cord-007211-prygoc0q	220	37	29	29	CD
cord-007211-prygoc0q	220	38	)	)	-RRB-
cord-007211-prygoc0q	220	39	.	.	.
cord-007211-prygoc0q	221	1	Since	since	IN
cord-007211-prygoc0q	221	2	the	the	DT
cord-007211-prygoc0q	221	3	formation	formation	NN
cord-007211-prygoc0q	221	4	of	of	IN
cord-007211-prygoc0q	221	5	the	the	DT
cord-007211-prygoc0q	221	6	correct	correct	JJ
cord-007211-prygoc0q	221	7	intramolecular	intramolecular	JJ
cord-007211-prygoc0q	221	8	disulfide	disulfide	NN
cord-007211-prygoc0q	221	9	bonds	bond	NNS
cord-007211-prygoc0q	221	10	seems	seem	VBZ
cord-007211-prygoc0q	221	11	to	to	TO
cord-007211-prygoc0q	221	12	be	be	VB
cord-007211-prygoc0q	221	13	a	a	DT
cord-007211-prygoc0q	221	14	complex	complex	JJ
cord-007211-prygoc0q	221	15	process	process	NN
cord-007211-prygoc0q	221	16	involving	involve	VBG
cord-007211-prygoc0q	221	17	disruption	disruption	NN
cord-007211-prygoc0q	221	18	and	and	CC
cord-007211-prygoc0q	221	19	rearrangement	rearrangement	NN
cord-007211-prygoc0q	221	20	of	of	IN
cord-007211-prygoc0q	221	21	disulfide	disulfide	JJ
cord-007211-prygoc0q	221	22	bonds	bond	NNS
cord-007211-prygoc0q	221	23	during	during	IN
cord-007211-prygoc0q	221	24	intracellular	intracellular	JJ
cord-007211-prygoc0q	221	25	transport	transport	NN
cord-007211-prygoc0q	221	26	,	,	,
cord-007211-prygoc0q	221	27	the	the	DT
cord-007211-prygoc0q	221	28	three	three	CD
cord-007211-prygoc0q	221	29	-	-	HYPH
cord-007211-prygoc0q	221	30	dimensional	dimensional	JJ
cord-007211-prygoc0q	221	31	structure	structure	NN
cord-007211-prygoc0q	221	32	of	of	IN
cord-007211-prygoc0q	221	33	these	these	DT
cord-007211-prygoc0q	221	34	cysteine	cysteine	NN
cord-007211-prygoc0q	221	35	mutants	mutant	NNS
cord-007211-prygoc0q	221	36	was	be	VBD
cord-007211-prygoc0q	221	37	changed	change	VBN
cord-007211-prygoc0q	221	38	,	,	,
cord-007211-prygoc0q	221	39	and	and	CC
cord-007211-prygoc0q	221	40	the	the	DT
cord-007211-prygoc0q	221	41	immunoreactivity	immunoreactivity	NN
cord-007211-prygoc0q	221	42	of	of	IN
cord-007211-prygoc0q	221	43	MAbs	MAbs	NNP
cord-007211-prygoc0q	221	44	with	with	IN
cord-007211-prygoc0q	221	45	these	these	DT
cord-007211-prygoc0q	221	46	mutant	mutant	JJ
cord-007211-prygoc0q	221	47	proteins	protein	NNS
cord-007211-prygoc0q	221	48	decreased	decrease	VBD
cord-007211-prygoc0q	221	49	dramatically	dramatically	RB
cord-007211-prygoc0q	221	50	.	.	.
cord-007211-prygoc0q	222	1	These	these	DT
cord-007211-prygoc0q	222	2	data	datum	NNS
cord-007211-prygoc0q	222	3	suggested	suggest	VBD
cord-007211-prygoc0q	222	4	that	that	IN
cord-007211-prygoc0q	222	5	co	co	JJ
cord-007211-prygoc0q	222	6	-	-	JJ
cord-007211-prygoc0q	222	7	translational	translational	JJ
cord-007211-prygoc0q	222	8	disulfide	disulfide	NN
cord-007211-prygoc0q	222	9	bond	bond	NN
cord-007211-prygoc0q	222	10	formation	formation	NN
cord-007211-prygoc0q	222	11	is	be	VBZ
cord-007211-prygoc0q	222	12	an	an	DT
cord-007211-prygoc0q	222	13	absolute	absolute	JJ
cord-007211-prygoc0q	222	14	requirement	requirement	NN
cord-007211-prygoc0q	222	15	for	for	IN
cord-007211-prygoc0q	222	16	subsequent	subsequent	JJ
cord-007211-prygoc0q	222	17	folding	folding	NN
cord-007211-prygoc0q	222	18	.	.	.
cord-007211-prygoc0q	223	1	Because	because	IN
cord-007211-prygoc0q	223	2	of	of	IN
cord-007211-prygoc0q	223	3	the	the	DT
cord-007211-prygoc0q	223	4	incorrect	incorrect	JJ
cord-007211-prygoc0q	223	5	folding	folding	NN
cord-007211-prygoc0q	223	6	of	of	IN
cord-007211-prygoc0q	223	7	cysteine	cysteine	NN
cord-007211-prygoc0q	223	8	mutant	mutant	JJ
cord-007211-prygoc0q	223	9	F	F	NNP
cord-007211-prygoc0q	223	10	proteins	protein	NNS
cord-007211-prygoc0q	223	11	,	,	,
cord-007211-prygoc0q	223	12	their	-PRON-	PRP$
cord-007211-prygoc0q	223	13	transport	transport	NN
cord-007211-prygoc0q	223	14	to	to	IN
cord-007211-prygoc0q	223	15	the	the	DT
cord-007211-prygoc0q	223	16	cell	cell	NN
cord-007211-prygoc0q	223	17	surface	surface	NN
cord-007211-prygoc0q	223	18	was	be	VBD
cord-007211-prygoc0q	223	19	reduced	reduce	VBN
cord-007211-prygoc0q	223	20	drastically	drastically	RB
cord-007211-prygoc0q	223	21	and	and	CC
cord-007211-prygoc0q	223	22	they	-PRON-	PRP
cord-007211-prygoc0q	223	23	contained	contain	VBD
cord-007211-prygoc0q	223	24	only	only	RB
cord-007211-prygoc0q	223	25	high	high	JJ
cord-007211-prygoc0q	223	26	mannose	mannose	NN
cord-007211-prygoc0q	223	27	-	-	HYPH
cord-007211-prygoc0q	223	28	type	type	NN
cord-007211-prygoc0q	223	29	oligosaccharides	oligosaccharide	NNS
cord-007211-prygoc0q	223	30	.	.	.
cord-007211-prygoc0q	224	1	These	these	DT
cord-007211-prygoc0q	224	2	results	result	NNS
cord-007211-prygoc0q	224	3	indicated	indicate	VBD
cord-007211-prygoc0q	224	4	that	that	IN
cord-007211-prygoc0q	224	5	the	the	DT
cord-007211-prygoc0q	224	6	mutant	mutant	JJ
cord-007211-prygoc0q	224	7	proteins	protein	NNS
cord-007211-prygoc0q	224	8	were	be	VBD
cord-007211-prygoc0q	224	9	retained	retain	VBN
cord-007211-prygoc0q	224	10	in	in	IN
cord-007211-prygoc0q	224	11	the	the	DT
cord-007211-prygoc0q	224	12	ER	ER	NNP
cord-007211-prygoc0q	224	13	.	.	.
cord-007211-prygoc0q	225	1	Viral	viral	JJ
cord-007211-prygoc0q	225	2	glycoprotein	glycoprotein	NN
cord-007211-prygoc0q	225	3	mutants	mutant	NNS
cord-007211-prygoc0q	225	4	that	that	WDT
cord-007211-prygoc0q	225	5	had	have	VBD
cord-007211-prygoc0q	225	6	a	a	DT
cord-007211-prygoc0q	225	7	temperature	temperature	NN
cord-007211-prygoc0q	225	8	-	-	HYPH
cord-007211-prygoc0q	225	9	sensitive	sensitive	JJ
cord-007211-prygoc0q	225	10	phenotype	phenotype	NN
cord-007211-prygoc0q	225	11	and	and	CC
cord-007211-prygoc0q	225	12	folded	fold	VBD
cord-007211-prygoc0q	225	13	correctly	correctly	RB
cord-007211-prygoc0q	225	14	at	at	IN
cord-007211-prygoc0q	225	15	32	32	CD
cord-007211-prygoc0q	225	16	°	°	NN
cord-007211-prygoc0q	225	17	C	c	NN
cord-007211-prygoc0q	225	18	but	but	CC
cord-007211-prygoc0q	225	19	misfolded	misfolde	VBN
cord-007211-prygoc0q	225	20	at	at	IN
cord-007211-prygoc0q	225	21	37	37	CD
cord-007211-prygoc0q	225	22	°	°	NN
cord-007211-prygoc0q	225	23	C	c	NN
cord-007211-prygoc0q	225	24	have	have	VBP
cord-007211-prygoc0q	225	25	been	be	VBN
cord-007211-prygoc0q	225	26	reported	report	VBN
cord-007211-prygoc0q	225	27	(	(	-LRB-
cord-007211-prygoc0q	225	28	4	4	CD
cord-007211-prygoc0q	225	29	)	)	-RRB-
cord-007211-prygoc0q	225	30	.	.	.
cord-007211-prygoc0q	226	1	In	in	IN
cord-007211-prygoc0q	226	2	HSV-1	hsv-1	NN
cord-007211-prygoc0q	226	3	,	,	,
cord-007211-prygoc0q	226	4	the	the	DT
cord-007211-prygoc0q	226	5	cysteine	cysteine	NN
cord-007211-prygoc0q	226	6	mutant	mutant	NN
cord-007211-prygoc0q	226	7	gD	gd	CD
cord-007211-prygoc0q	226	8	proteins	protein	NNS
cord-007211-prygoc0q	226	9	showed	show	VBD
cord-007211-prygoc0q	226	10	temperature	temperature	NN
cord-007211-prygoc0q	226	11	sensitive	sensitive	JJ
cord-007211-prygoc0q	226	12	cell	cell	NN
cord-007211-prygoc0q	226	13	surface	surface	NN
cord-007211-prygoc0q	226	14	expression	expression	NN
cord-007211-prygoc0q	226	15	(	(	-LRB-
cord-007211-prygoc0q	226	16	12	12	CD
cord-007211-prygoc0q	226	17	)	)	-RRB-
cord-007211-prygoc0q	226	18	.	.	.
cord-007211-prygoc0q	227	1	We	-PRON-	PRP
cord-007211-prygoc0q	227	2	examined	examine	VBD
cord-007211-prygoc0q	227	3	whether	whether	IN
cord-007211-prygoc0q	227	4	cysteine	cysteine	NN
cord-007211-prygoc0q	227	5	mutant	mutant	JJ
cord-007211-prygoc0q	227	6	Sendai	Sendai	NNP
cord-007211-prygoc0q	227	7	virus	virus	NN
cord-007211-prygoc0q	227	8	F	F	NNP
cord-007211-prygoc0q	227	9	proteins	protein	NNS
cord-007211-prygoc0q	227	10	show	show	VBP
cord-007211-prygoc0q	227	11	temperature	temperature	NN
cord-007211-prygoc0q	227	12	sensitivity	sensitivity	NN
cord-007211-prygoc0q	227	13	,	,	,
cord-007211-prygoc0q	227	14	but	but	CC
cord-007211-prygoc0q	227	15	we	-PRON-	PRP
cord-007211-prygoc0q	227	16	could	could	MD
cord-007211-prygoc0q	227	17	not	not	RB
cord-007211-prygoc0q	227	18	detect	detect	VB
cord-007211-prygoc0q	227	19	cell	cell	NN
cord-007211-prygoc0q	227	20	surface	surface	NN
cord-007211-prygoc0q	227	21	expression	expression	NN
cord-007211-prygoc0q	227	22	of	of	IN
cord-007211-prygoc0q	227	23	the	the	DT
cord-007211-prygoc0q	227	24	cysteine	cysteine	NN
cord-007211-prygoc0q	227	25	mutant	mutant	NN
cord-007211-prygoc0q	227	26	F	F	NNP
cord-007211-prygoc0q	227	27	proteins	protein	NNS
cord-007211-prygoc0q	227	28	by	by	IN
cord-007211-prygoc0q	227	29	incubating	incubate	VBG
cord-007211-prygoc0q	227	30	mutant	mutant	JJ
cord-007211-prygoc0q	227	31	gene	gene	NN
cord-007211-prygoc0q	227	32	-	-	HYPH
cord-007211-prygoc0q	227	33	expressing	express	VBG
cord-007211-prygoc0q	227	34	cells	cell	NNS
cord-007211-prygoc0q	227	35	at	at	IN
cord-007211-prygoc0q	227	36	33	33	CD
cord-007211-prygoc0q	227	37	°	°	NN
cord-007211-prygoc0q	227	38	C	c	NN
cord-007211-prygoc0q	227	39	or	or	CC
cord-007211-prygoc0q	227	40	at	at	IN
cord-007211-prygoc0q	227	41	31.5	31.5	CD
cord-007211-prygoc0q	227	42	°	°	NN
cord-007211-prygoc0q	227	43	C	C	NNP
cord-007211-prygoc0q	227	44	(	(	-LRB-
cord-007211-prygoc0q	227	45	data	datum	NNS
cord-007211-prygoc0q	227	46	not	not	RB
cord-007211-prygoc0q	227	47	shown	show	VBN
cord-007211-prygoc0q	227	48	)	)	-RRB-
cord-007211-prygoc0q	227	49	.	.	.
cord-007211-prygoc0q	228	1	These	these	DT
cord-007211-prygoc0q	228	2	results	result	NNS
cord-007211-prygoc0q	228	3	suggest	suggest	VBP
cord-007211-prygoc0q	228	4	that	that	IN
cord-007211-prygoc0q	228	5	the	the	DT
cord-007211-prygoc0q	228	6	inhibition	inhibition	NN
cord-007211-prygoc0q	228	7	of	of	IN
cord-007211-prygoc0q	228	8	the	the	DT
cord-007211-prygoc0q	228	9	expression	expression	NN
cord-007211-prygoc0q	228	10	of	of	IN
cord-007211-prygoc0q	228	11	the	the	DT
cord-007211-prygoc0q	228	12	cysteine	cysteine	NN
cord-007211-prygoc0q	228	13	mutant	mutant	NN
cord-007211-prygoc0q	228	14	F	F	NNP
cord-007211-prygoc0q	228	15	proteins	protein	NNS
cord-007211-prygoc0q	228	16	on	on	IN
cord-007211-prygoc0q	228	17	the	the	DT
cord-007211-prygoc0q	228	18	cell	cell	NN
cord-007211-prygoc0q	228	19	surface	surface	NN
cord-007211-prygoc0q	228	20	occurred	occur	VBD
cord-007211-prygoc0q	228	21	at	at	IN
cord-007211-prygoc0q	228	22	a	a	DT
cord-007211-prygoc0q	228	23	stage	stage	NN
cord-007211-prygoc0q	228	24	involved	involve	VBN
cord-007211-prygoc0q	228	25	in	in	IN
cord-007211-prygoc0q	228	26	the	the	DT
cord-007211-prygoc0q	228	27	intracellular	intracellular	JJ
cord-007211-prygoc0q	228	28	transport	transport	NN
cord-007211-prygoc0q	228	29	.	.	.
cord-007211-prygoc0q	229	1	Though	though	IN
cord-007211-prygoc0q	229	2	many	many	JJ
cord-007211-prygoc0q	229	3	reports	report	NNS
cord-007211-prygoc0q	229	4	have	have	VBP
cord-007211-prygoc0q	229	5	shown	show	VBN
cord-007211-prygoc0q	229	6	that	that	IN
cord-007211-prygoc0q	229	7	cysteine	cysteine	NN
cord-007211-prygoc0q	229	8	mutant	mutant	JJ
cord-007211-prygoc0q	229	9	proteins	protein	NNS
cord-007211-prygoc0q	229	10	are	be	VBP
cord-007211-prygoc0q	229	11	improperly	improperly	RB
cord-007211-prygoc0q	229	12	folded	fold	VBN
cord-007211-prygoc0q	229	13	and	and	CC
cord-007211-prygoc0q	229	14	found	find	VBN
cord-007211-prygoc0q	229	15	as	as	IN
cord-007211-prygoc0q	229	16	disulfide	disulfide	NN
cord-007211-prygoc0q	229	17	-linked	-linked	JJ
cord-007211-prygoc0q	229	18	aggregates	aggregate	NNS
cord-007211-prygoc0q	229	19	in	in	IN
cord-007211-prygoc0q	229	20	the	the	DT
cord-007211-prygoc0q	229	21	ER	ER	NNP
cord-007211-prygoc0q	229	22	,	,	,
cord-007211-prygoc0q	229	23	there	there	EX
cord-007211-prygoc0q	229	24	are	be	VBP
cord-007211-prygoc0q	229	25	some	some	DT
cord-007211-prygoc0q	229	26	reports	report	NNS
cord-007211-prygoc0q	229	27	that	that	IN
cord-007211-prygoc0q	229	28	cysteine	cysteine	NN
cord-007211-prygoc0q	229	29	mutant	mutant	JJ
cord-007211-prygoc0q	229	30	proteins	protein	NNS
cord-007211-prygoc0q	229	31	could	could	MD
cord-007211-prygoc0q	229	32	be	be	VB
cord-007211-prygoc0q	229	33	transported	transport	VBN
cord-007211-prygoc0q	229	34	to	to	IN
cord-007211-prygoc0q	229	35	the	the	DT
cord-007211-prygoc0q	229	36	cell	cell	NN
cord-007211-prygoc0q	229	37	surface	surface	NN
cord-007211-prygoc0q	229	38	,	,	,
cord-007211-prygoc0q	229	39	e.g.	e.g.	RB
cord-007211-prygoc0q	229	40	,	,	,
cord-007211-prygoc0q	229	41	Newcastle	Newcastle	NNP
cord-007211-prygoc0q	229	42	disease	disease	NN
cord-007211-prygoc0q	229	43	virus	virus	NN
cord-007211-prygoc0q	229	44	(	(	-LRB-
cord-007211-prygoc0q	229	45	NDV	NDV	NNP
cord-007211-prygoc0q	229	46	)	)	-RRB-
cord-007211-prygoc0q	230	1	HN	HN	NNP
cord-007211-prygoc0q	230	2	,	,	,
cord-007211-prygoc0q	230	3	measles	measles	NN
cord-007211-prygoc0q	230	4	virus	virus	NN
cord-007211-prygoc0q	230	5	H	H	NNP
cord-007211-prygoc0q	230	6	,	,	,
cord-007211-prygoc0q	230	7	and	and	CC
cord-007211-prygoc0q	230	8	asialoglycoprotein	asialoglycoprotein	NNP
cord-007211-prygoc0q	230	9	receptor	receptor	NN
cord-007211-prygoc0q	230	10	H2b	h2b	XX
cord-007211-prygoc0q	230	11	subunit	subunit	NN
cord-007211-prygoc0q	230	12	,	,	,
cord-007211-prygoc0q	230	13	all	all	DT
cord-007211-prygoc0q	230	14	of	of	IN
cord-007211-prygoc0q	230	15	which	which	WDT
cord-007211-prygoc0q	230	16	have	have	VBP
cord-007211-prygoc0q	230	17	type	type	NN
cord-007211-prygoc0q	230	18	II	ii	CD
cord-007211-prygoc0q	230	19	topology	topology	NN
cord-007211-prygoc0q	230	20	(	(	-LRB-
cord-007211-prygoc0q	230	21	5	5	CD
cord-007211-prygoc0q	230	22	,	,	,
cord-007211-prygoc0q	230	23	6	6	CD
cord-007211-prygoc0q	230	24	,	,	,
cord-007211-prygoc0q	230	25	14	14	CD
cord-007211-prygoc0q	230	26	)	)	-RRB-
cord-007211-prygoc0q	230	27	.	.	.
cord-007211-prygoc0q	231	1	It	-PRON-	PRP
cord-007211-prygoc0q	231	2	seems	seem	VBZ
cord-007211-prygoc0q	231	3	that	that	DT
cord-007211-prygoc0q	231	4	type	type	NN
cord-007211-prygoc0q	231	5	I	-PRON-	PRP
cord-007211-prygoc0q	231	6	transmembrane	transmembrane	NN
cord-007211-prygoc0q	231	7	proteins	protein	NNS
cord-007211-prygoc0q	231	8	more	more	RBR
cord-007211-prygoc0q	231	9	strictly	strictly	RB
cord-007211-prygoc0q	231	10	require	require	VBP
cord-007211-prygoc0q	231	11	disulfide	disulfide	JJ
cord-007211-prygoc0q	231	12	bond	bond	NN
cord-007211-prygoc0q	231	13	formation	formation	NN
cord-007211-prygoc0q	231	14	than	than	IN
cord-007211-prygoc0q	231	15	do	do	VB
cord-007211-prygoc0q	231	16	type	type	NN
cord-007211-prygoc0q	231	17	II	II	NNP
cord-007211-prygoc0q	231	18	proteins	protein	NNS
cord-007211-prygoc0q	231	19	in	in	IN
cord-007211-prygoc0q	231	20	order	order	NN
cord-007211-prygoc0q	231	21	to	to	TO
cord-007211-prygoc0q	231	22	exit	exit	VB
cord-007211-prygoc0q	231	23	from	from	IN
cord-007211-prygoc0q	231	24	the	the	DT
cord-007211-prygoc0q	231	25	ER	ER	NNP
cord-007211-prygoc0q	231	26	.	.	.
cord-007211-prygoc0q	232	1	We	-PRON-	PRP
cord-007211-prygoc0q	232	2	previously	previously	RB
cord-007211-prygoc0q	232	3	found	find	VBD
cord-007211-prygoc0q	232	4	that	that	IN
cord-007211-prygoc0q	232	5	cleavage	cleavage	NN
cord-007211-prygoc0q	232	6	of	of	IN
cord-007211-prygoc0q	232	7	an	an	DT
cord-007211-prygoc0q	232	8	intrinsically	intrinsically	RB
cord-007211-prygoc0q	232	9	protease	protease	NN
cord-007211-prygoc0q	232	10	-	-	HYPH
cord-007211-prygoc0q	232	11	sensitive	sensitive	JJ
cord-007211-prygoc0q	232	12	mutant	mutant	JJ
cord-007211-prygoc0q	232	13	F	f	NN
cord-007211-prygoc0q	232	14	,	,	,
cord-007211-prygoc0q	232	15	Fmut	Fmut	NNP
cord-007211-prygoc0q	232	16	,	,	,
cord-007211-prygoc0q	232	17	was	be	VBD
cord-007211-prygoc0q	232	18	enhanced	enhance	VBN
cord-007211-prygoc0q	232	19	by	by	IN
cord-007211-prygoc0q	232	20	coexpression	coexpression	NN
cord-007211-prygoc0q	232	21	with	with	IN
cord-007211-prygoc0q	232	22	HN	HN	NNP
cord-007211-prygoc0q	232	23	protein	protein	NN
cord-007211-prygoc0q	232	24	(	(	-LRB-
cord-007211-prygoc0q	232	25	16	16	CD
cord-007211-prygoc0q	232	26	)	)	-RRB-
cord-007211-prygoc0q	232	27	,	,	,
cord-007211-prygoc0q	232	28	indicating	indicate	VBG
cord-007211-prygoc0q	232	29	that	that	IN
cord-007211-prygoc0q	232	30	F	F	NNP
cord-007211-prygoc0q	232	31	protein	protein	NN
cord-007211-prygoc0q	232	32	associates	associate	NNS
cord-007211-prygoc0q	232	33	with	with	IN
cord-007211-prygoc0q	232	34	HN	HN	NNP
cord-007211-prygoc0q	232	35	protein	protein	NN
cord-007211-prygoc0q	232	36	intracellulary	intracellulary	NNP
cord-007211-prygoc0q	232	37	.	.	.
cord-007211-prygoc0q	233	1	Thus	thus	RB
cord-007211-prygoc0q	233	2	,	,	,
cord-007211-prygoc0q	233	3	we	-PRON-	PRP
cord-007211-prygoc0q	233	4	examined	examine	VBD
cord-007211-prygoc0q	233	5	the	the	DT
cord-007211-prygoc0q	233	6	effect	effect	NN
cord-007211-prygoc0q	233	7	of	of	IN
cord-007211-prygoc0q	233	8	co	co	JJ
cord-007211-prygoc0q	233	9	-	-	NN
cord-007211-prygoc0q	233	10	expression	expression	NN
cord-007211-prygoc0q	233	11	of	of	IN
cord-007211-prygoc0q	233	12	HN	HN	NNP
cord-007211-prygoc0q	233	13	on	on	IN
cord-007211-prygoc0q	233	14	cell	cell	NN
cord-007211-prygoc0q	233	15	surface	surface	NN
cord-007211-prygoc0q	233	16	expression	expression	NN
cord-007211-prygoc0q	233	17	of	of	IN
cord-007211-prygoc0q	233	18	cysteine	cysteine	NN
cord-007211-prygoc0q	233	19	mutant	mutant	JJ
cord-007211-prygoc0q	233	20	F	F	NNP
cord-007211-prygoc0q	233	21	proteins	protein	NNS
cord-007211-prygoc0q	233	22	.	.	.
cord-007211-prygoc0q	234	1	Although	although	IN
cord-007211-prygoc0q	234	2	the	the	DT
cord-007211-prygoc0q	234	3	expression	expression	NN
cord-007211-prygoc0q	234	4	of	of	IN
cord-007211-prygoc0q	234	5	both	both	DT
cord-007211-prygoc0q	234	6	proteins	protein	NNS
cord-007211-prygoc0q	234	7	was	be	VBD
cord-007211-prygoc0q	234	8	detected	detect	VBN
cord-007211-prygoc0q	234	9	at	at	IN
cord-007211-prygoc0q	234	10	roughly	roughly	RB
cord-007211-prygoc0q	234	11	equal	equal	JJ
cord-007211-prygoc0q	234	12	levels	level	NNS
cord-007211-prygoc0q	234	13	by	by	IN
cord-007211-prygoc0q	234	14	Western	western	JJ
cord-007211-prygoc0q	234	15	blot	blot	NN
cord-007211-prygoc0q	234	16	analysis	analysis	NN
cord-007211-prygoc0q	235	1	(	(	-LRB-
cord-007211-prygoc0q	235	2	Fig	Fig	NNP
cord-007211-prygoc0q	235	3	.	.	.
cord-007211-prygoc0q	235	4	5B	5b	CD
cord-007211-prygoc0q	235	5	)	)	-RRB-
cord-007211-prygoc0q	235	6	,	,	,
cord-007211-prygoc0q	235	7	cell	cell	NN
cord-007211-prygoc0q	235	8	surface	surface	NN
cord-007211-prygoc0q	235	9	expression	expression	NN
cord-007211-prygoc0q	235	10	of	of	IN
cord-007211-prygoc0q	235	11	cysteine	cysteine	NN
cord-007211-prygoc0q	235	12	mutant	mutant	JJ
cord-007211-prygoc0q	235	13	F	F	NNP
cord-007211-prygoc0q	235	14	proteins	protein	NNS
cord-007211-prygoc0q	235	15	was	be	VBD
cord-007211-prygoc0q	235	16	not	not	RB
cord-007211-prygoc0q	235	17	detected	detect	VBN
cord-007211-prygoc0q	235	18	,	,	,
cord-007211-prygoc0q	235	19	and	and	CC
cord-007211-prygoc0q	235	20	a	a	DT
cord-007211-prygoc0q	235	21	decrease	decrease	NN
cord-007211-prygoc0q	235	22	of	of	IN
cord-007211-prygoc0q	235	23	cell	cell	NN
cord-007211-prygoc0q	235	24	surface	surface	NN
cord-007211-prygoc0q	235	25	expression	expression	NN
cord-007211-prygoc0q	235	26	of	of	IN
cord-007211-prygoc0q	235	27	HN	HN	NNP
cord-007211-prygoc0q	235	28	protein	protein	NN
cord-007211-prygoc0q	235	29	was	be	VBD
cord-007211-prygoc0q	235	30	observed	observe	VBN
cord-007211-prygoc0q	235	31	(	(	-LRB-
cord-007211-prygoc0q	235	32	Fig	Fig	NNP
cord-007211-prygoc0q	235	33	.	.	.
cord-007211-prygoc0q	235	34	5A	5a	CD
cord-007211-prygoc0q	235	35	)	)	-RRB-
cord-007211-prygoc0q	235	36	.	.	.
cord-007211-prygoc0q	236	1	These	these	DT
cord-007211-prygoc0q	236	2	observations	observation	NNS
cord-007211-prygoc0q	236	3	are	be	VBP
cord-007211-prygoc0q	236	4	in	in	IN
cord-007211-prygoc0q	236	5	accord	accord	NN
cord-007211-prygoc0q	236	6	with	with	IN
cord-007211-prygoc0q	236	7	the	the	DT
cord-007211-prygoc0q	236	8	down	down	JJ
cord-007211-prygoc0q	236	9	-	-	HYPH
cord-007211-prygoc0q	236	10	regulation	regulation	NN
cord-007211-prygoc0q	236	11	of	of	IN
cord-007211-prygoc0q	236	12	the	the	DT
cord-007211-prygoc0q	236	13	human	human	JJ
cord-007211-prygoc0q	236	14	parainfiuenza	parainfiuenza	NN
cord-007211-prygoc0q	236	15	type	type	NNP
cord-007211-prygoc0q	236	16	3	3	NNP
cord-007211-prygoc0q	236	17	(	(	-LRB-
cord-007211-prygoc0q	236	18	HPIV3	HPIV3	NNP
cord-007211-prygoc0q	236	19	)	)	-RRB-
cord-007211-prygoc0q	237	1	HN	HN	NNP
cord-007211-prygoc0q	237	2	protein	protein	NN
cord-007211-prygoc0q	237	3	cell	cell	NN
cord-007211-prygoc0q	237	4	surface	surface	NN
cord-007211-prygoc0q	237	5	expression	expression	NN
cord-007211-prygoc0q	237	6	by	by	IN
cord-007211-prygoc0q	237	7	the	the	DT
cord-007211-prygoc0q	237	8	mutant	mutant	JJ
cord-007211-prygoc0q	237	9	F	F	NNP
cord-007211-prygoc0q	237	10	protein	protein	NN
cord-007211-prygoc0q	237	11	containing	contain	VBG
cord-007211-prygoc0q	237	12	an	an	DT
cord-007211-prygoc0q	237	13	intracellular	intracellular	JJ
cord-007211-prygoc0q	237	14	retention	retention	NN
cord-007211-prygoc0q	237	15	signal	signal	NN
cord-007211-prygoc0q	237	16	(	(	-LRB-
cord-007211-prygoc0q	237	17	30	30	CD
cord-007211-prygoc0q	237	18	)	)	-RRB-
cord-007211-prygoc0q	237	19	.	.	.
cord-007211-prygoc0q	238	1	Recently	recently	RB
cord-007211-prygoc0q	238	2	,	,	,
cord-007211-prygoc0q	238	3	many	many	JJ
cord-007211-prygoc0q	238	4	reports	report	NNS
cord-007211-prygoc0q	238	5	have	have	VBP
cord-007211-prygoc0q	238	6	shown	show	VBN
cord-007211-prygoc0q	238	7	a	a	DT
cord-007211-prygoc0q	238	8	direct	direct	JJ
cord-007211-prygoc0q	238	9	interaction	interaction	NN
cord-007211-prygoc0q	238	10	of	of	IN
cord-007211-prygoc0q	238	11	paramyxovirus	paramyxovirus	NNP
cord-007211-prygoc0q	238	12	F	F	NNP
cord-007211-prygoc0q	238	13	and	and	CC
cord-007211-prygoc0q	238	14	HN	HN	NNP
cord-007211-prygoc0q	238	15	proteins	protein	NNS
cord-007211-prygoc0q	238	16	(	(	-LRB-
cord-007211-prygoc0q	238	17	31	31	CD
cord-007211-prygoc0q	238	18	)	)	-RRB-
cord-007211-prygoc0q	238	19	(	(	-LRB-
cord-007211-prygoc0q	238	20	32	32	CD
cord-007211-prygoc0q	238	21	)	)	-RRB-
cord-007211-prygoc0q	238	22	(	(	-LRB-
cord-007211-prygoc0q	238	23	33	33	CD
cord-007211-prygoc0q	238	24	)	)	-RRB-
cord-007211-prygoc0q	238	25	(	(	-LRB-
cord-007211-prygoc0q	238	26	34	34	CD
cord-007211-prygoc0q	238	27	)	)	-RRB-
cord-007211-prygoc0q	238	28	.	.	.
cord-007211-prygoc0q	239	1	However	however	RB
cord-007211-prygoc0q	239	2	,	,	,
cord-007211-prygoc0q	239	3	the	the	DT
cord-007211-prygoc0q	239	4	cell	cell	NN
cord-007211-prygoc0q	239	5	surface	surface	NN
cord-007211-prygoc0q	239	6	expression	expression	NN
cord-007211-prygoc0q	239	7	of	of	IN
cord-007211-prygoc0q	239	8	F	F	NNP
cord-007211-prygoc0q	239	9	and	and	CC
cord-007211-prygoc0q	239	10	HN	HN	NNP
cord-007211-prygoc0q	239	11	proteins	protein	NNS
cord-007211-prygoc0q	239	12	is	be	VBZ
cord-007211-prygoc0q	239	13	reported	report	VBN
cord-007211-prygoc0q	239	14	to	to	TO
cord-007211-prygoc0q	239	15	occur	occur	VB
cord-007211-prygoc0q	239	16	at	at	IN
cord-007211-prygoc0q	239	17	different	different	JJ
cord-007211-prygoc0q	239	18	rates	rate	NNS
cord-007211-prygoc0q	239	19	because	because	IN
cord-007211-prygoc0q	239	20	of	of	IN
cord-007211-prygoc0q	239	21	the	the	DT
cord-007211-prygoc0q	239	22	different	different	JJ
cord-007211-prygoc0q	239	23	degrees	degree	NNS
cord-007211-prygoc0q	239	24	of	of	IN
cord-007211-prygoc0q	239	25	association	association	NN
cord-007211-prygoc0q	239	26	with	with	IN
cord-007211-prygoc0q	239	27	BiP	BiP	NNP
cord-007211-prygoc0q	239	28	(	(	-LRB-
cord-007211-prygoc0q	239	29	35	35	CD
cord-007211-prygoc0q	239	30	)	)	-RRB-
cord-007211-prygoc0q	239	31	.	.	.
cord-007211-prygoc0q	240	1	Thus	thus	RB
cord-007211-prygoc0q	240	2	,	,	,
cord-007211-prygoc0q	240	3	decrease	decrease	NN
cord-007211-prygoc0q	240	4	of	of	IN
cord-007211-prygoc0q	240	5	cell	cell	NN
cord-007211-prygoc0q	240	6	surface	surface	NN
cord-007211-prygoc0q	240	7	expression	expression	NN
cord-007211-prygoc0q	240	8	of	of	IN
cord-007211-prygoc0q	240	9	HN	HN	NNP
cord-007211-prygoc0q	240	10	protein	protein	NN
cord-007211-prygoc0q	240	11	in	in	IN
cord-007211-prygoc0q	240	12	the	the	DT
cord-007211-prygoc0q	240	13	cysteine	cysteine	NN
cord-007211-prygoc0q	240	14	mutants	mutant	NNS
cord-007211-prygoc0q	241	1	coexpressing	coexpressing	NN
cord-007211-prygoc0q	241	2	cells	cell	NNS
cord-007211-prygoc0q	241	3	may	may	MD
cord-007211-prygoc0q	241	4	be	be	VB
cord-007211-prygoc0q	241	5	caused	cause	VBN
cord-007211-prygoc0q	241	6	not	not	RB
cord-007211-prygoc0q	241	7	only	only	RB
cord-007211-prygoc0q	241	8	by	by	IN
cord-007211-prygoc0q	241	9	direct	direct	JJ
cord-007211-prygoc0q	241	10	interactions	interaction	NNS
cord-007211-prygoc0q	241	11	of	of	IN
cord-007211-prygoc0q	241	12	the	the	DT
cord-007211-prygoc0q	241	13	F	F	NNP
cord-007211-prygoc0q	241	14	and	and	CC
cord-007211-prygoc0q	241	15	HN	HN	NNP
cord-007211-prygoc0q	241	16	proteins	protein	NNS
cord-007211-prygoc0q	241	17	but	but	CC
cord-007211-prygoc0q	241	18	also	also	RB
cord-007211-prygoc0q	241	19	by	by	IN
cord-007211-prygoc0q	241	20	indirect	indirect	JJ
cord-007211-prygoc0q	241	21	interaction	interaction	NN
cord-007211-prygoc0q	241	22	affected	affect	VBN
cord-007211-prygoc0q	241	23	by	by	IN
cord-007211-prygoc0q	241	24	binding	bind	VBG
cord-007211-prygoc0q	241	25	of	of	IN
cord-007211-prygoc0q	241	26	molecular	molecular	JJ
cord-007211-prygoc0q	241	27	chaperons	chaperon	NNS
cord-007211-prygoc0q	241	28	such	such	JJ
cord-007211-prygoc0q	241	29	as	as	IN
cord-007211-prygoc0q	241	30	BiP	BiP	NNP
cord-007211-prygoc0q	241	31	or	or	CC
cord-007211-prygoc0q	241	32	calnexin	calnexin	NNP
cord-007211-prygoc0q	241	33	.	.	.
cord-007211-prygoc0q	242	1	Morrison	Morrison	NNP
cord-007211-prygoc0q	242	2	et	et	NNP
cord-007211-prygoc0q	242	3	al	al	NNP
cord-007211-prygoc0q	242	4	.	.	NNP
cord-007211-prygoc0q	242	5	reported	report	VBD
cord-007211-prygoc0q	242	6	that	that	IN
cord-007211-prygoc0q	242	7	NDV	NDV	NNP
cord-007211-prygoc0q	242	8	F	F	NNP
cord-007211-prygoc0q	242	9	protein	protein	NN
cord-007211-prygoc0q	242	10	undergoes	undergo	VBZ
cord-007211-prygoc0q	242	11	conformational	conformational	JJ
cord-007211-prygoc0q	242	12	change	change	NN
cord-007211-prygoc0q	242	13	with	with	IN
cord-007211-prygoc0q	242	14	disruption	disruption	NN
cord-007211-prygoc0q	242	15	and	and	CC
cord-007211-prygoc0q	242	16	rearrangement	rearrangement	NN
cord-007211-prygoc0q	242	17	of	of	IN
cord-007211-prygoc0q	242	18	disulfide	disulfide	JJ
cord-007211-prygoc0q	242	19	bonds	bond	NNS
cord-007211-prygoc0q	242	20	during	during	IN
cord-007211-prygoc0q	242	21	intracellular	intracellular	JJ
cord-007211-prygoc0q	242	22	transport	transport	NN
cord-007211-prygoc0q	242	23	,	,	,
cord-007211-prygoc0q	242	24	and	and	CC
cord-007211-prygoc0q	242	25	that	that	IN
cord-007211-prygoc0q	242	26	the	the	DT
cord-007211-prygoc0q	242	27	conformational	conformational	JJ
cord-007211-prygoc0q	242	28	change	change	NN
cord-007211-prygoc0q	242	29	occurs	occur	VBZ
cord-007211-prygoc0q	242	30	before	before	IN
cord-007211-prygoc0q	242	31	the	the	DT
cord-007211-prygoc0q	242	32	cleavage	cleavage	NN
cord-007211-prygoc0q	242	33	reaction	reaction	NN
cord-007211-prygoc0q	242	34	(	(	-LRB-
cord-007211-prygoc0q	242	35	36	36	CD
cord-007211-prygoc0q	242	36	)	)	-RRB-
cord-007211-prygoc0q	242	37	.	.	.
cord-007211-prygoc0q	243	1	Because	because	IN
cord-007211-prygoc0q	243	2	of	of	IN
cord-007211-prygoc0q	243	3	the	the	DT
cord-007211-prygoc0q	243	4	intracellular	intracellular	JJ
cord-007211-prygoc0q	243	5	transport	transport	NN
cord-007211-prygoc0q	243	6	deficiency	deficiency	NN
cord-007211-prygoc0q	243	7	of	of	IN
cord-007211-prygoc0q	243	8	the	the	DT
cord-007211-prygoc0q	243	9	cysteine	cysteine	NN
cord-007211-prygoc0q	243	10	mutant	mutant	NN
cord-007211-prygoc0q	243	11	F	F	NNP
cord-007211-prygoc0q	243	12	proteins	protein	NNS
cord-007211-prygoc0q	243	13	,	,	,
cord-007211-prygoc0q	243	14	we	-PRON-	PRP
cord-007211-prygoc0q	243	15	could	could	MD
cord-007211-prygoc0q	243	16	not	not	RB
cord-007211-prygoc0q	243	17	identify	identify	VB
cord-007211-prygoc0q	243	18	the	the	DT
cord-007211-prygoc0q	243	19	cysteine	cysteine	NN
cord-007211-prygoc0q	243	20	residues	residue	NNS
cord-007211-prygoc0q	243	21	involved	involve	VBN
cord-007211-prygoc0q	243	22	in	in	IN
cord-007211-prygoc0q	243	23	such	such	JJ
cord-007211-prygoc0q	243	24	disulfide	disulfide	JJ
cord-007211-prygoc0q	243	25	bond	bond	NN
cord-007211-prygoc0q	243	26	rearrangements	rearrangement	NNS
cord-007211-prygoc0q	243	27	.	.	.
cord-007211-prygoc0q	244	1	To	to	TO
cord-007211-prygoc0q	244	2	study	study	VB
cord-007211-prygoc0q	244	3	sequential	sequential	JJ
cord-007211-prygoc0q	244	4	disulfide	disulfide	JJ
cord-007211-prygoc0q	244	5	bond	bond	NN
cord-007211-prygoc0q	244	6	formation	formation	NN
cord-007211-prygoc0q	244	7	and	and	CC
cord-007211-prygoc0q	244	8	bond	bond	NN
cord-007211-prygoc0q	244	9	rearrangements	rearrangement	NNS
cord-007211-prygoc0q	244	10	,	,	,
cord-007211-prygoc0q	244	11	in	in	IN
cord-007211-prygoc0q	244	12	vivo	vivo	NN
cord-007211-prygoc0q	244	13	reduction	reduction	NN
cord-007211-prygoc0q	244	14	experiments	experiment	NNS
cord-007211-prygoc0q	244	15	using	use	VBG
cord-007211-prygoc0q	244	16	well	well	RB
cord-007211-prygoc0q	244	17	-	-	HYPH
cord-007211-prygoc0q	244	18	characterized	characterize	VBN
cord-007211-prygoc0q	244	19	MAbs	MAbs	NNP
cord-007211-prygoc0q	244	20	are	be	VBP
cord-007211-prygoc0q	244	21	also	also	RB
cord-007211-prygoc0q	244	22	now	now	RB
cord-007211-prygoc0q	244	23	under	under	IN
cord-007211-prygoc0q	244	24	way	way	NN
cord-007211-prygoc0q	244	25	.	.	.
cord-007211-prygoc0q	245	1	Paramyxovirus	Paramyxovirus	NNP
cord-007211-prygoc0q	245	2	fusion	fusion	NN
cord-007211-prygoc0q	245	3	:	:	:
cord-007211-prygoc0q	245	4	a	a	DT
cord-007211-prygoc0q	245	5	hypothesis	hypothesis	NN
cord-007211-prygoc0q	245	6	of	of	IN
cord-007211-prygoc0q	245	7	changes	change	NNS
cord-007211-prygoc0q	245	8	The	the	DT
cord-007211-prygoc0q	245	9	Paramyxoviruses	Paramyxoviruses	NNPS
cord-007211-prygoc0q	245	10	Determination	Determination	NNP
cord-007211-prygoc0q	245	11	of	of	IN
cord-007211-prygoc0q	245	12	the	the	DT
cord-007211-prygoc0q	245	13	complete	complete	JJ
cord-007211-prygoc0q	245	14	nucleotide	nucleotide	JJ
cord-007211-prygoc0q	245	15	sequence	sequence	NN
cord-007211-prygoc0q	245	16	of	of	IN
cord-007211-prygoc0q	245	17	the	the	DT
cord-007211-prygoc0q	245	18	Sendai	Sendai	NNP
cord-007211-prygoc0q	245	19	virus	virus	NN
cord-007211-prygoc0q	245	20	genome	genome	NN
cord-007211-prygoc0q	245	21	RNA	RNA	NNP
cord-007211-prygoc0q	245	22	and	and	CC
cord-007211-prygoc0q	245	23	the	the	DT
cord-007211-prygoc0q	245	24	predicted	predict	VBN
cord-007211-prygoc0q	245	25	amino	amino	NN
cord-007211-prygoc0q	245	26	acid	acid	NN
cord-007211-prygoc0q	245	27	sequences	sequence	NNS
cord-007211-prygoc0q	245	28	of	of	IN
cord-007211-prygoc0q	245	29	the	the	DT
cord-007211-prygoc0q	245	30	F	F	NNP
cord-007211-prygoc0q	245	31	,	,	,
cord-007211-prygoc0q	245	32	HN	HN	NNP
cord-007211-prygoc0q	245	33	and	and	CC
cord-007211-prygoc0q	245	34	L	L	NNP
cord-007211-prygoc0q	245	35	protein	protein	NN
cord-007211-prygoc0q	245	36	Folding	folding	NN
cord-007211-prygoc0q	245	37	and	and	CC
cord-007211-prygoc0q	245	38	assembly	assembly	NN
cord-007211-prygoc0q	245	39	of	of	IN
cord-007211-prygoc0q	245	40	viral	viral	JJ
cord-007211-prygoc0q	245	41	membrane	membrane	NN
cord-007211-prygoc0q	245	42	proteins	protein	NNS
cord-007211-prygoc0q	246	1	The	the	DT
cord-007211-prygoc0q	246	2	role	role	NN
cord-007211-prygoc0q	246	3	of	of	IN
cord-007211-prygoc0q	246	4	the	the	DT
cord-007211-prygoc0q	246	5	individual	individual	JJ
cord-007211-prygoc0q	246	6	cysteine	cysteine	NN
cord-007211-prygoc0q	246	7	residues	residue	NNS
cord-007211-prygoc0q	246	8	in	in	IN
cord-007211-prygoc0q	246	9	the	the	DT
cord-007211-prygoc0q	246	10	formation	formation	NN
cord-007211-prygoc0q	246	11	of	of	IN
cord-007211-prygoc0q	246	12	the	the	DT
cord-007211-prygoc0q	246	13	mature	mature	JJ
cord-007211-prygoc0q	246	14	,	,	,
cord-007211-prygoc0q	246	15	antigenic	antigenic	JJ
cord-007211-prygoc0q	246	16	HN	HN	NNP
cord-007211-prygoc0q	246	17	protein	protein	NN
cord-007211-prygoc0q	246	18	of	of	IN
cord-007211-prygoc0q	246	19	Newcastle	Newcastle	NNP
cord-007211-prygoc0q	246	20	disease	disease	NN
cord-007211-prygoc0q	246	21	virus	virus	NN
cord-007211-prygoc0q	246	22	Role	role	NN
cord-007211-prygoc0q	246	23	of	of	IN
cord-007211-prygoc0q	246	24	individual	individual	JJ
cord-007211-prygoc0q	246	25	cysteine	cysteine	NN
cord-007211-prygoc0q	246	26	residues	residue	NNS
cord-007211-prygoc0q	246	27	in	in	IN
cord-007211-prygoc0q	246	28	the	the	DT
cord-007211-prygoc0q	246	29	processing	processing	NN
cord-007211-prygoc0q	246	30	and	and	CC
cord-007211-prygoc0q	246	31	antigenicity	antigenicity	NN
cord-007211-prygoc0q	246	32	of	of	IN
cord-007211-prygoc0q	246	33	the	the	DT
cord-007211-prygoc0q	246	34	measles	measles	NNP
cord-007211-prygoc0q	246	35	virus	virus	NN
cord-007211-prygoc0q	246	36	haemagglutinin	haemagglutinin	NNP
cord-007211-prygoc0q	246	37	protein	protein	NNP
cord-007211-prygoc0q	246	38	ing	ing	DT
cord-007211-prygoc0q	246	39	disulfide	disulfide	JJ
cord-007211-prygoc0q	246	40	bond	bond	NN
cord-007211-prygoc0q	246	41	formation	formation	NN
cord-007211-prygoc0q	246	42	and	and	CC
cord-007211-prygoc0q	246	43	protein	protein	NN
cord-007211-prygoc0q	246	44	folding	folding	NN
cord-007211-prygoc0q	246	45	in	in	IN
cord-007211-prygoc0q	246	46	the	the	DT
cord-007211-prygoc0q	246	47	endoplasmic	endoplasmic	NN
cord-007211-prygoc0q	246	48	reticulum	reticulum	NN
cord-007211-prygoc0q	246	49	Quality	Quality	NNP
cord-007211-prygoc0q	246	50	control	control	NN
cord-007211-prygoc0q	246	51	in	in	IN
cord-007211-prygoc0q	246	52	the	the	DT
cord-007211-prygoc0q	246	53	endoplasmic	endoplasmic	NN
cord-007211-prygoc0q	246	54	reticulum	reticulum	NN
cord-007211-prygoc0q	246	55	folding	folding	NN
cord-007211-prygoc0q	246	56	and	and	CC
cord-007211-prygoc0q	246	57	misfolding	misfolding	NN
cord-007211-prygoc0q	246	58	of	of	IN
cord-007211-prygoc0q	246	59	vesicular	vesicular	JJ
cord-007211-prygoc0q	246	60	stomatitis	stomatitis	NN
cord-007211-prygoc0q	246	61	virus	virus	NN
cord-007211-prygoc0q	246	62	G	g	NN
cord-007211-prygoc0q	246	63	protein	protein	NN
cord-007211-prygoc0q	246	64	in	in	IN
cord-007211-prygoc0q	246	65	cells	cell	NNS
cord-007211-prygoc0q	246	66	in	in	IN
cord-007211-prygoc0q	246	67	vitro	vitro	FW
cord-007211-prygoc0q	246	68	Role	role	NN
cord-007211-prygoc0q	246	69	of	of	IN
cord-007211-prygoc0q	246	70	cotranslational	cotranslational	NNP
cord-007211-prygoc0q	246	71	disulfide	disulfide	NNP
cord-007211-prygoc0q	246	72	bond	bond	NN
cord-007211-prygoc0q	246	73	formation	formation	NN
cord-007211-prygoc0q	246	74	in	in	IN
cord-007211-prygoc0q	246	75	the	the	DT
cord-007211-prygoc0q	246	76	folding	folding	NN
cord-007211-prygoc0q	246	77	of	of	IN
cord-007211-prygoc0q	246	78	the	the	DT
cord-007211-prygoc0q	246	79	hemagglutinin	hemagglutinin	NN
cord-007211-prygoc0q	246	80	-	-	HYPH
cord-007211-prygoc0q	246	81	neuraminidase	neuraminidase	NN
cord-007211-prygoc0q	246	82	protein	protein	NN
cord-007211-prygoc0q	246	83	of	of	IN
cord-007211-prygoc0q	246	84	Newcastle	Newcastle	NNP
cord-007211-prygoc0q	246	85	disease	disease	NN
cord-007211-prygoc0q	246	86	virus	virus	NN
cord-007211-prygoc0q	246	87	Disulfide	Disulfide	NNP
cord-007211-prygoc0q	246	88	bonds	bond	NNS
cord-007211-prygoc0q	246	89	in	in	IN
cord-007211-prygoc0q	246	90	folding	folding	NN
cord-007211-prygoc0q	246	91	and	and	CC
cord-007211-prygoc0q	246	92	transport	transport	NN
cord-007211-prygoc0q	246	93	of	of	IN
cord-007211-prygoc0q	246	94	mouse	mouse	NN
cord-007211-prygoc0q	246	95	hepatitis	hepatitis	NN
cord-007211-prygoc0q	246	96	coronavirus	coronavirus	NN
cord-007211-prygoc0q	246	97	glycoproteins	glycoprotein	VBZ
cord-007211-prygoc0q	246	98	Cysteine	cysteine	NN
cord-007211-prygoc0q	246	99	mutants	mutant	NNS
cord-007211-prygoc0q	246	100	of	of	IN
cord-007211-prygoc0q	246	101	herpes	herpe	NNS
cord-007211-prygoc0q	246	102	simplex	simplex	NNP
cord-007211-prygoc0q	246	103	virus	virus	NN
cord-007211-prygoc0q	246	104	type	type	NN
cord-007211-prygoc0q	246	105	1	1	CD
cord-007211-prygoc0q	246	106	glycoprotein	glycoprotein	NN
cord-007211-prygoc0q	246	107	D	d	NN
cord-007211-prygoc0q	246	108	exhibit	exhibit	NN
cord-007211-prygoc0q	246	109	temperature	temperature	NN
cord-007211-prygoc0q	246	110	-	-	HYPH
cord-007211-prygoc0q	246	111	sensitive	sensitive	JJ
cord-007211-prygoc0q	246	112	properties	property	NNS
cord-007211-prygoc0q	246	113	in	in	IN
cord-007211-prygoc0q	246	114	structure	structure	NN
cord-007211-prygoc0q	246	115	and	and	CC
cord-007211-prygoc0q	246	116	function	function	VB
cord-007211-prygoc0q	246	117	Disulfide	Disulfide	NNP
cord-007211-prygoc0q	246	118	bond	bond	NN
cord-007211-prygoc0q	246	119	structure	structure	NN
cord-007211-prygoc0q	246	120	of	of	IN
cord-007211-prygoc0q	246	121	glycoprotein	glycoprotein	NN
cord-007211-prygoc0q	246	122	D	D	NNP
cord-007211-prygoc0q	246	123	of	of	IN
cord-007211-prygoc0q	246	124	herpes	herpes	NNP
cord-007211-prygoc0q	246	125	simplex	simplex	NNP
cord-007211-prygoc0q	246	126	virus	virus	NN
cord-007211-prygoc0q	246	127	types	type	NNS
cord-007211-prygoc0q	246	128	1	1	CD
cord-007211-prygoc0q	246	129	and	and	CC
cord-007211-prygoc0q	246	130	2	2	CD
cord-007211-prygoc0q	247	1	The	the	DT
cord-007211-prygoc0q	247	2	contribution	contribution	NN
cord-007211-prygoc0q	247	3	of	of	IN
cord-007211-prygoc0q	247	4	cysteine	cysteine	NN
cord-007211-prygoc0q	247	5	residues	residue	NNS
cord-007211-prygoc0q	247	6	to	to	IN
cord-007211-prygoc0q	247	7	antigenicity	antigenicity	NN
cord-007211-prygoc0q	247	8	and	and	CC
cord-007211-prygoc0q	247	9	extent	extent	NN
cord-007211-prygoc0q	247	10	of	of	IN
cord-007211-prygoc0q	247	11	processing	processing	NN
cord-007211-prygoc0q	247	12	of	of	IN
cord-007211-prygoc0q	247	13	herpes	herpes	NNP
cord-007211-prygoc0q	247	14	simplex	simplex	NNP
cord-007211-prygoc0q	247	15	virus	virus	NN
cord-007211-prygoc0q	247	16	type	type	NN
cord-007211-prygoc0q	247	17	1	1	CD
cord-007211-prygoc0q	247	18	glycoprotein	glycoprotein	NN
cord-007211-prygoc0q	247	19	D	D	NNP
cord-007211-prygoc0q	247	20	Enhanced	enhance	VBN
cord-007211-prygoc0q	247	21	folding	folding	NN
cord-007211-prygoc0q	247	22	and	and	CC
cord-007211-prygoc0q	247	23	processing	processing	NN
cord-007211-prygoc0q	247	24	of	of	IN
cord-007211-prygoc0q	247	25	a	a	DT
cord-007211-prygoc0q	247	26	disulfide	disulfide	NN
cord-007211-prygoc0q	247	27	mutant	mutant	NN
cord-007211-prygoc0q	247	28	of	of	IN
cord-007211-prygoc0q	247	29	the	the	DT
cord-007211-prygoc0q	247	30	human	human	JJ
cord-007211-prygoc0q	247	31	asialoglycoprotein	asialoglycoprotein	NNP
cord-007211-prygoc0q	247	32	receptor	receptor	NN
cord-007211-prygoc0q	247	33	H2b	H2b	NNP
cord-007211-prygoc0q	247	34	subunit	subunit	NN
cord-007211-prygoc0q	247	35	Assignment	assignment	NN
cord-007211-prygoc0q	247	36	of	of	IN
cord-007211-prygoc0q	247	37	disulfide	disulfide	JJ
cord-007211-prygoc0q	247	38	bridges	bridge	NNS
cord-007211-prygoc0q	247	39	in	in	IN
cord-007211-prygoc0q	247	40	the	the	DT
cord-007211-prygoc0q	247	41	fusion	fusion	NN
cord-007211-prygoc0q	247	42	glycoprotein	glycoprotein	NN
cord-007211-prygoc0q	247	43	of	of	IN
cord-007211-prygoc0q	247	44	Sendai	Sendai	NNP
cord-007211-prygoc0q	247	45	virus	virus	NN
cord-007211-prygoc0q	247	46	Transfection	Transfection	NNP
cord-007211-prygoc0q	247	47	of	of	IN
cord-007211-prygoc0q	247	48	Sendai	Sendai	NNP
cord-007211-prygoc0q	247	49	virus	virus	NN
cord-007211-prygoc0q	247	50	F	F	NNP
cord-007211-prygoc0q	247	51	gene	gene	NN
cord-007211-prygoc0q	247	52	cDNA	cDNA	NNP
cord-007211-prygoc0q	247	53	with	with	IN
cord-007211-prygoc0q	247	54	mutations	mutation	NNS
cord-007211-prygoc0q	247	55	at	at	IN
cord-007211-prygoc0q	247	56	its	-PRON-	PRP$
cord-007211-prygoc0q	247	57	cleavage	cleavage	NN
cord-007211-prygoc0q	247	58	site	site	NN
cord-007211-prygoc0q	247	59	and	and	CC
cord-007211-prygoc0q	247	60	HN	HN	NNP
cord-007211-prygoc0q	247	61	gene	gene	NN
cord-007211-prygoc0q	247	62	cDNA	cdna	NN
cord-007211-prygoc0q	247	63	into	into	IN
cord-007211-prygoc0q	247	64	COS	COS	NNP
cord-007211-prygoc0q	247	65	cells	cell	NNS
cord-007211-prygoc0q	247	66	induces	induce	VBZ
cord-007211-prygoc0q	247	67	cell	cell	NN
cord-007211-prygoc0q	247	68	fusion	fusion	NN
cord-007211-prygoc0q	247	69	A	a	DT
cord-007211-prygoc0q	247	70	general	general	JJ
cord-007211-prygoc0q	247	71	method	method	NN
cord-007211-prygoc0q	247	72	for	for	IN
cord-007211-prygoc0q	247	73	rapid	rapid	JJ
cord-007211-prygoc0q	247	74	site	site	NN
cord-007211-prygoc0q	247	75	-	-	HYPH
cord-007211-prygoc0q	247	76	directed	direct	VBN
cord-007211-prygoc0q	247	77	mutagenesis	mutagenesis	NN
cord-007211-prygoc0q	247	78	using	use	VBG
cord-007211-prygoc0q	247	79	the	the	DT
cord-007211-prygoc0q	247	80	polymerase	polymerase	NN
cord-007211-prygoc0q	247	81	chain	chain	NN
cord-007211-prygoc0q	247	82	reaction	reaction	NN
cord-007211-prygoc0q	247	83	Construction	construction	NN
cord-007211-prygoc0q	247	84	of	of	IN
cord-007211-prygoc0q	247	85	expression	expression	NN
cord-007211-prygoc0q	247	86	plasmids	plasmid	NNS
cord-007211-prygoc0q	247	87	for	for	IN
cord-007211-prygoc0q	247	88	the	the	DT
cord-007211-prygoc0q	247	89	fusion	fusion	NN
cord-007211-prygoc0q	247	90	protein	protein	NN
cord-007211-prygoc0q	247	91	of	of	IN
cord-007211-prygoc0q	247	92	Sendai	Sendai	NNP
cord-007211-prygoc0q	247	93	virus	virus	NN
cord-007211-prygoc0q	247	94	,	,	,
cord-007211-prygoc0q	247	95	and	and	CC
cord-007211-prygoc0q	247	96	their	-PRON-	PRP$
cord-007211-prygoc0q	247	97	expression	expression	NN
cord-007211-prygoc0q	247	98	in	in	IN
cord-007211-prygoc0q	247	99	E.	E.	NNP
cord-007211-prygoc0q	247	100	coli	coli	NNS
cord-007211-prygoc0q	247	101	and	and	CC
cord-007211-prygoc0q	247	102	eucaryotic	eucaryotic	JJ
cord-007211-prygoc0q	247	103	cells	cell	NNS
cord-007211-prygoc0q	247	104	SRa	sra	RB
cord-007211-prygoc0q	247	105	promoter	promoter	NN
cord-007211-prygoc0q	247	106	:	:	:
cord-007211-prygoc0q	247	107	an	an	DT
cord-007211-prygoc0q	247	108	efficient	efficient	JJ
cord-007211-prygoc0q	247	109	and	and	CC
cord-007211-prygoc0q	247	110	versatile	versatile	JJ
cord-007211-prygoc0q	247	111	mammalian	mammalian	JJ
cord-007211-prygoc0q	247	112	cDNA	cdna	NN
cord-007211-prygoc0q	247	113	expression	expression	NN
cord-007211-prygoc0q	247	114	system	system	NN
cord-007211-prygoc0q	247	115	composed	compose	VBN
cord-007211-prygoc0q	247	116	of	of	IN
cord-007211-prygoc0q	247	117	the	the	DT
cord-007211-prygoc0q	247	118	simian	simian	JJ
cord-007211-prygoc0q	247	119	virus	virus	NN
cord-007211-prygoc0q	247	120	40	40	CD
cord-007211-prygoc0q	247	121	early	early	JJ
cord-007211-prygoc0q	247	122	promoter	promoter	NN
cord-007211-prygoc0q	247	123	and	and	CC
cord-007211-prygoc0q	247	124	the	the	DT
cord-007211-prygoc0q	247	125	R	R	NNP
cord-007211-prygoc0q	247	126	-	-	HYPH
cord-007211-prygoc0q	247	127	U5	u5	NN
cord-007211-prygoc0q	247	128	segment	segment	NN
cord-007211-prygoc0q	247	129	of	of	IN
cord-007211-prygoc0q	247	130	the	the	DT
cord-007211-prygoc0q	247	131	human	human	JJ
cord-007211-prygoc0q	247	132	T	t	NN
cord-007211-prygoc0q	247	133	-	-	HYPH
cord-007211-prygoc0q	247	134	cell	cell	NN
cord-007211-prygoc0q	247	135	leukemia	leukemia	NN
cord-007211-prygoc0q	247	136	virus	virus	NN
cord-007211-prygoc0q	247	137	type	type	NN
cord-007211-prygoc0q	247	138	1	1	CD
cord-007211-prygoc0q	247	139	long	long	JJ
cord-007211-prygoc0q	247	140	terminal	terminal	NN
cord-007211-prygoc0q	247	141	repeat	repeat	NN
cord-007211-prygoc0q	247	142	DNA	dna	NN
cord-007211-prygoc0q	247	143	sequencing	sequencing	NN
cord-007211-prygoc0q	247	144	with	with	IN
cord-007211-prygoc0q	247	145	chain	chain	NN
cord-007211-prygoc0q	247	146	-	-	HYPH
cord-007211-prygoc0q	247	147	terminating	terminate	VBG
cord-007211-prygoc0q	247	148	inhibitors	inhibitor	NNS
cord-007211-prygoc0q	247	149	SV40-transformed	sv40-transformed	CD
cord-007211-prygoc0q	247	150	simian	simian	JJ
cord-007211-prygoc0q	247	151	cells	cell	NNS
cord-007211-prygoc0q	247	152	support	support	VBP
cord-007211-prygoc0q	247	153	the	the	DT
cord-007211-prygoc0q	247	154	replication	replication	NN
cord-007211-prygoc0q	247	155	of	of	IN
cord-007211-prygoc0q	247	156	early	early	JJ
cord-007211-prygoc0q	247	157	SV40	SV40	NNP
cord-007211-prygoc0q	247	158	mutants	mutant	NNS
cord-007211-prygoc0q	247	159	A	a	DT
cord-007211-prygoc0q	247	160	new	new	JJ
cord-007211-prygoc0q	247	161	technique	technique	NN
cord-007211-prygoc0q	247	162	for	for	IN
cord-007211-prygoc0q	247	163	the	the	DT
cord-007211-prygoc0q	247	164	assay	assay	NN
cord-007211-prygoc0q	247	165	of	of	IN
cord-007211-prygoc0q	247	166	infectivity	infectivity	NN
cord-007211-prygoc0q	247	167	of	of	IN
cord-007211-prygoc0q	247	168	human	human	JJ
cord-007211-prygoc0q	247	169	adenovirus	adenovirus	NN
cord-007211-prygoc0q	247	170	5	5	CD
cord-007211-prygoc0q	247	171	DNA	DNA	NNP
cord-007211-prygoc0q	247	172	Cleavage	cleavage	NN
cord-007211-prygoc0q	247	173	of	of	IN
cord-007211-prygoc0q	247	174	structural	structural	JJ
cord-007211-prygoc0q	247	175	proteins	protein	NNS
cord-007211-prygoc0q	247	176	during	during	IN
cord-007211-prygoc0q	247	177	the	the	DT
cord-007211-prygoc0q	247	178	assembly	assembly	NN
cord-007211-prygoc0q	247	179	of	of	IN
cord-007211-prygoc0q	247	180	the	the	DT
cord-007211-prygoc0q	247	181	head	head	NN
cord-007211-prygoc0q	247	182	of	of	IN
cord-007211-prygoc0q	247	183	bacteriophage	bacteriophage	NN
cord-007211-prygoc0q	247	184	T4	T4	NNP
cord-007211-prygoc0q	247	185	Conformational	Conformational	NNP
cord-007211-prygoc0q	247	186	aberrance	aberrance	NN
cord-007211-prygoc0q	247	187	of	of	IN
cord-007211-prygoc0q	247	188	Sendai	Sendai	NNP
cord-007211-prygoc0q	247	189	virus	virus	NN
cord-007211-prygoc0q	247	190	F0	f0	NN
cord-007211-prygoc0q	247	191	protein	protein	NN
cord-007211-prygoc0q	247	192	in	in	IN
cord-007211-prygoc0q	247	193	thapsigargin	thapsigargin	RB
cord-007211-prygoc0q	247	194	-	-	HYPH
cord-007211-prygoc0q	247	195	treated	treat	VBN
cord-007211-prygoc0q	247	196	cells	cell	NNS
cord-007211-prygoc0q	247	197	allowing	allow	VBG
cord-007211-prygoc0q	247	198	exit	exit	NN
cord-007211-prygoc0q	247	199	from	from	IN
cord-007211-prygoc0q	247	200	the	the	DT
cord-007211-prygoc0q	247	201	endoplasmic	endoplasmic	NN
cord-007211-prygoc0q	247	202	reticulum	reticulum	NN
cord-007211-prygoc0q	247	203	but	but	CC
cord-007211-prygoc0q	247	204	causing	cause	VBG
cord-007211-prygoc0q	247	205	arrest	arrest	NN
cord-007211-prygoc0q	247	206	at	at	IN
cord-007211-prygoc0q	247	207	the	the	DT
cord-007211-prygoc0q	247	208	Golgi	Golgi	NNP
cord-007211-prygoc0q	247	209	complex	complex	NN
cord-007211-prygoc0q	247	210	Neutralizing	neutralize	VBG
cord-007211-prygoc0q	247	211	activity	activity	NN
cord-007211-prygoc0q	247	212	of	of	IN
cord-007211-prygoc0q	247	213	the	the	DT
cord-007211-prygoc0q	247	214	antibodies	antibody	NNS
cord-007211-prygoc0q	247	215	against	against	IN
cord-007211-prygoc0q	247	216	two	two	CD
cord-007211-prygoc0q	247	217	kinds	kind	NNS
cord-007211-prygoc0q	247	218	of	of	IN
cord-007211-prygoc0q	247	219	envelope	envelope	NN
cord-007211-prygoc0q	247	220	glycoproteins	glycoprotein	NNS
cord-007211-prygoc0q	247	221	of	of	IN
cord-007211-prygoc0q	247	222	Sendai	Sendai	NNP
cord-007211-prygoc0q	247	223	virus	virus	NN
cord-007211-prygoc0q	247	224	Expression	expression	NN
cord-007211-prygoc0q	247	225	of	of	IN
cord-007211-prygoc0q	247	226	the	the	DT
cord-007211-prygoc0q	247	227	Sendai	Sendai	NNP
cord-007211-prygoc0q	247	228	virus	virus	NN
cord-007211-prygoc0q	247	229	fusion	fusion	NN
cord-007211-prygoc0q	247	230	protein	protein	NN
cord-007211-prygoc0q	247	231	and	and	CC
cord-007211-prygoc0q	247	232	the	the	DT
cord-007211-prygoc0q	247	233	hemagglutinin	hemagglutinin	NN
cord-007211-prygoc0q	247	234	-	-	HYPH
cord-007211-prygoc0q	247	235	neuraminidase	neuraminidase	NN
cord-007211-prygoc0q	247	236	protein	protein	NN
cord-007211-prygoc0q	247	237	using	use	VBG
cord-007211-prygoc0q	247	238	a	a	DT
cord-007211-prygoc0q	247	239	baculovirus	baculovirus	NN
cord-007211-prygoc0q	247	240	vector	vector	NN
cord-007211-prygoc0q	247	241	Heavy	Heavy	NNP
cord-007211-prygoc0q	247	242	chain	chain	NN
cord-007211-prygoc0q	247	243	binding	bind	VBG
cord-007211-prygoc0q	247	244	protein	protein	NN
cord-007211-prygoc0q	247	245	recognizes	recognize	VBZ
cord-007211-prygoc0q	247	246	incompletely	incompletely	RB
cord-007211-prygoc0q	247	247	disulfide	disulfide	RB
cord-007211-prygoc0q	247	248	-	-	HYPH
cord-007211-prygoc0q	247	249	bonded	bond	VBN
cord-007211-prygoc0q	247	250	forms	form	NNS
cord-007211-prygoc0q	247	251	of	of	IN
cord-007211-prygoc0q	247	252	vesicular	vesicular	JJ
cord-007211-prygoc0q	247	253	stomatitis	stomatitis	NN
cord-007211-prygoc0q	247	254	virus	virus	NN
cord-007211-prygoc0q	247	255	G	g	NN
cord-007211-prygoc0q	247	256	protein	protein	NN
cord-007211-prygoc0q	247	257	Carbohydrate	carbohydrate	NN
cord-007211-prygoc0q	247	258	structures	structure	NNS
cord-007211-prygoc0q	247	259	of	of	IN
cord-007211-prygoc0q	247	260	HVJ	HVJ	NNP
cord-007211-prygoc0q	247	261	(	(	-LRB-
cord-007211-prygoc0q	247	262	Sendai	Sendai	NNP
cord-007211-prygoc0q	247	263	virus	virus	NN
cord-007211-prygoc0q	247	264	)	)	-RRB-
cord-007211-prygoc0q	247	265	glycoproteins	glycoprotein	VBZ
cord-007211-prygoc0q	247	266	Perturbation	perturbation	NN
cord-007211-prygoc0q	247	267	of	of	IN
cord-007211-prygoc0q	247	268	cellular	cellular	JJ
cord-007211-prygoc0q	247	269	calcium	calcium	NN
cord-007211-prygoc0q	247	270	blocks	block	VBZ
cord-007211-prygoc0q	247	271	exit	exit	NN
cord-007211-prygoc0q	247	272	of	of	IN
cord-007211-prygoc0q	247	273	secretory	secretory	JJ
cord-007211-prygoc0q	247	274	proteins	protein	NNS
cord-007211-prygoc0q	247	275	from	from	IN
cord-007211-prygoc0q	247	276	the	the	DT
cord-007211-prygoc0q	247	277	rough	rough	JJ
cord-007211-prygoc0q	247	278	endoplasmic	endoplasmic	NN
cord-007211-prygoc0q	247	279	reticulum	reticulum	NN
cord-007211-prygoc0q	247	280	Downregulation	Downregulation	NNP
cord-007211-prygoc0q	247	281	of	of	IN
cord-007211-prygoc0q	247	282	paramyxovirus	paramyxovirus	JJ
cord-007211-prygoc0q	247	283	hemagglutinin	hemagglutinin	NN
cord-007211-prygoc0q	247	284	-	-	HYPH
cord-007211-prygoc0q	247	285	neuraminidase	neuraminidase	NN
cord-007211-prygoc0q	247	286	glycoprotein	glycoprotein	NN
cord-007211-prygoc0q	247	287	surface	surface	NN
cord-007211-prygoc0q	247	288	expression	expression	NN
cord-007211-prygoc0q	247	289	by	by	IN
cord-007211-prygoc0q	247	290	a	a	DT
cord-007211-prygoc0q	247	291	mutant	mutant	JJ
cord-007211-prygoc0q	247	292	fusion	fusion	NN
cord-007211-prygoc0q	247	293	protein	protein	NN
cord-007211-prygoc0q	247	294	containing	contain	VBG
cord-007211-prygoc0q	247	295	a	a	DT
cord-007211-prygoc0q	247	296	retention	retention	NN
cord-007211-prygoc0q	247	297	signal	signal	NN
cord-007211-prygoc0q	247	298	for	for	IN
cord-007211-prygoc0q	247	299	the	the	DT
cord-007211-prygoc0q	247	300	endoplasmic	endoplasmic	NN
cord-007211-prygoc0q	247	301	reticulum	reticulum	NN
cord-007211-prygoc0q	247	302	Identification	identification	NN
cord-007211-prygoc0q	247	303	of	of	IN
cord-007211-prygoc0q	247	304	regions	region	NNS
cord-007211-prygoc0q	247	305	on	on	IN
cord-007211-prygoc0q	247	306	the	the	DT
cord-007211-prygoc0q	247	307	hemagglutinin	hemagglutinin	NN
cord-007211-prygoc0q	247	308	-	-	HYPH
cord-007211-prygoc0q	247	309	neuraminidase	neuraminidase	NN
cord-007211-prygoc0q	247	310	protein	protein	NN
cord-007211-prygoc0q	247	311	of	of	IN
cord-007211-prygoc0q	247	312	human	human	NNP
cord-007211-prygoc0q	247	313	parainfluenza	parainfluenza	NNP
cord-007211-prygoc0q	247	314	virus	virus	NNP
cord-007211-prygoc0q	247	315	type	type	NN
cord-007211-prygoc0q	247	316	2	2	CD
cord-007211-prygoc0q	247	317	important	important	JJ
cord-007211-prygoc0q	247	318	for	for	IN
cord-007211-prygoc0q	247	319	promoting	promote	VBG
cord-007211-prygoc0q	247	320	cell	cell	NN
cord-007211-prygoc0q	247	321	fusion	fusion	NN
cord-007211-prygoc0q	247	322	Functional	functional	JJ
cord-007211-prygoc0q	247	323	interaction	interaction	NN
cord-007211-prygoc0q	247	324	of	of	IN
cord-007211-prygoc0q	247	325	paramyxovirus	paramyxovirus	JJ
cord-007211-prygoc0q	247	326	glycoproteins	glycoprotein	NNS
cord-007211-prygoc0q	247	327	:	:	:
cord-007211-prygoc0q	247	328	identification	identification	NN
cord-007211-prygoc0q	247	329	of	of	IN
cord-007211-prygoc0q	247	330	a	a	DT
cord-007211-prygoc0q	247	331	domain	domain	NN
cord-007211-prygoc0q	247	332	in	in	IN
cord-007211-prygoc0q	247	333	Sendai	Sendai	NNP
cord-007211-prygoc0q	247	334	virus	virus	NN
cord-007211-prygoc0q	247	335	HN	HN	NNP
cord-007211-prygoc0q	247	336	which	which	WDT
cord-007211-prygoc0q	247	337	promotes	promote	VBZ
cord-007211-prygoc0q	247	338	cell	cell	NN
cord-007211-prygoc0q	247	339	fusion	fusion	NN
cord-007211-prygoc0q	247	340	Association	Association	NNP
cord-007211-prygoc0q	247	341	of	of	IN
cord-007211-prygoc0q	247	342	the	the	DT
cord-007211-prygoc0q	247	343	parainfluenza	parainfluenza	NNP
cord-007211-prygoc0q	247	344	virus	virus	NNP
cord-007211-prygoc0q	247	345	fusion	fusion	NNP
cord-007211-prygoc0q	247	346	and	and	CC
cord-007211-prygoc0q	247	347	hemagglutinin	hemagglutinin	NN
cord-007211-prygoc0q	247	348	-	-	HYPH
cord-007211-prygoc0q	247	349	neuraminidase	neuraminidase	NN
cord-007211-prygoc0q	247	350	glycoproteins	glycoprotein	NNS
cord-007211-prygoc0q	247	351	on	on	IN
cord-007211-prygoc0q	247	352	cell	cell	NN
cord-007211-prygoc0q	247	353	surfaces	surface	NNS
cord-007211-prygoc0q	247	354	Detection	detection	NN
cord-007211-prygoc0q	247	355	of	of	IN
cord-007211-prygoc0q	247	356	an	an	DT
cord-007211-prygoc0q	247	357	interaction	interaction	NN
cord-007211-prygoc0q	247	358	between	between	IN
cord-007211-prygoc0q	247	359	the	the	DT
cord-007211-prygoc0q	247	360	HN	HN	NNP
cord-007211-prygoc0q	247	361	and	and	CC
cord-007211-prygoc0q	247	362	F	F	NNP
cord-007211-prygoc0q	247	363	proteins	protein	NNS
cord-007211-prygoc0q	247	364	in	in	IN
cord-007211-prygoc0q	247	365	Newcastle	Newcastle	NNP
cord-007211-prygoc0q	247	366	disease	disease	NN
cord-007211-prygoc0q	247	367	virus	virus	NN
cord-007211-prygoc0q	247	368	-	-	HYPH
cord-007211-prygoc0q	247	369	infected	infect	VBN
cord-007211-prygoc0q	247	370	cells	cell	NNS
cord-007211-prygoc0q	247	371	Selective	selective	JJ
cord-007211-prygoc0q	247	372	and	and	CC
cord-007211-prygoc0q	247	373	transient	transient	JJ
cord-007211-prygoc0q	247	374	association	association	NN
cord-007211-prygoc0q	247	375	of	of	IN
cord-007211-prygoc0q	247	376	Sendai	Sendai	NNP
cord-007211-prygoc0q	247	377	virus	virus	NN
cord-007211-prygoc0q	247	378	HN	HN	NNP
cord-007211-prygoc0q	247	379	glycoprotein	glycoprotein	NN
cord-007211-prygoc0q	247	380	with	with	IN
cord-007211-prygoc0q	247	381	BiP	BiP	NNP
cord-007211-prygoc0q	247	382	Conformational	Conformational	NNP
cord-007211-prygoc0q	247	383	change	change	VB
cord-007211-prygoc0q	247	384	in	in	IN
cord-007211-prygoc0q	247	385	a	a	DT
cord-007211-prygoc0q	247	386	viral	viral	JJ
cord-007211-prygoc0q	247	387	glycoprotein	glycoprotein	NN
cord-007211-prygoc0q	247	388	during	during	IN
cord-007211-prygoc0q	247	389	maturation	maturation	NN
cord-007211-prygoc0q	247	390	due	due	IN
cord-007211-prygoc0q	247	391	to	to	IN
cord-007211-prygoc0q	247	392	disulfide	disulfide	JJ
cord-007211-prygoc0q	247	393	bond	bond	NN
cord-007211-prygoc0q	247	394	disruption	disruption	NN