id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-337158-0iw2kcaf	Tiernan, Hannah	ATR-FTIR spectroscopy and spectroscopic imaging for the analysis of biopharmaceuticals	2020-06-22		.txt	text/plain	7405	425	38	Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR) spectroscopy is a label-free, non-destructive technique that can be applied to a vast range of biological applications, from imaging cancer tissues and live cells, to determining protein content and secondary structure composition. This characterisation commonly involves the use of techniques such as liquid chromatography tandem mass spectrometry (LC-MS-MS) to monitor changes in mass, 16 high pressure liquid chromatography (HPLC) to determine impurity profiles of samples, 17 and infrared spectroscopy (IR) to identify impurities and compare biosimilars to the original, 'reference drugs'. 75 ATR-FTIR spectroscopy has also been used extensively to study proteins, in particular biopharmaceuticals, and exciting research has shown the potential applications of it to investigate and monitor changes effectively in PTMs and secondary structures. For example, Grosshans et al used in-line FTIR spectroscopy, along with partial least squares (PLS) analysis, as an effective process analytical tool (PAT) for preparative protein chromatography, they found it to be useful to distinguish and selectively identify proteins based on their secondary structure.	./cache/cord-337158-0iw2kcaf.txt	./txt/cord-337158-0iw2kcaf.txt