id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-330668-7aw17jf8	Chen, Cheng-Chang	ORF8a of SARS-CoV forms an ion channel: Experiments and molecular dynamics simulations	2011-02-28		.txt	text/plain	4806	274	56	The protein is synthesized using solid phase peptide synthesis and reconstituted into artificial lipid bilayers that forms cation-selective ion channels with a main conductance level of 8.9±0.8pS at elevated temperature (38.5°C). Computational modeling studies including multi nanosecond molecular dynamics simulations in a hydrated POPC lipid bilayer are done with a 22 amino acid transmembrane helix to predict a putative homooligomeric helical bundle model. Before embedding low energy models into lipid bilayers two amino acids residues of the protein were added at the N and C termini of each of the helices in each bundle model to account for the consequences of their interaction with the lipid bilayer during the simulation. The idealized monomeric TM helix based on the consensus sequence Leu-3 to Val-20 (Fig. 1A) shows clustering of hydrophilic residues (Thr-8, Ser-11, Ser-14 and Thr-18) on one side suggesting that the four hydrophilic amino acids form the lumen of the pore in a homooligomeric helical bundle channel model.	./cache/cord-330668-7aw17jf8.txt	./txt/cord-330668-7aw17jf8.txt