id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-329403-jzrlywfe	Teo, Su Hui Catherine	A NS1-binding monoclonal antibody interacts with two residues that are highly conserved in seasonal as well as newly emerged influenza A virus	2019-03-06		.txt	text/plain	4448	246	57	In this study, we have generated a novel monoclonal antibody (mAb) 19H9 and epitope mapping revealed that two residues, P85 and Y89, of NS1 are essential for interacting with this mAb. Furthermore, residues P85 and Y89 are found to be highly conserved across different IAV subtypes, namely seasonal H1N1 and H3N2, as well as the highly pathogenic H5N1 and H5N6 avian strains. The membrane was then washed and full-length NS1 transfected cell lysates were subjected to IP with mAb 19H9 and protein A agarose beads followed by detection of immunoprecipitated proteins using rabbit anti-Myc antibody in Western blot. To delineate the epitope of NS1 recognized by mAb 19H9, a series of NS1 truncation mutants consisting of residues 1-84, 1-100, 91-230 and 85-230 were generated and their expression levels in transiently transfected 293T cells were verified using an anti-Myc antibody ( Fig. 2A, upper panel) .	./cache/cord-329403-jzrlywfe.txt	./txt/cord-329403-jzrlywfe.txt