id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-325943-3hvy7b7c	Bouwman, Kim M.	Guinea Fowl Coronavirus Diversity Has Phenotypic Consequences for Glycan and Tissue Binding	2019-05-01		.txt	text/plain	4810	265	51	Glycan array analysis revealed that, in addition to the previously elucidated biantennary di-N-acetyllactosamine (diLacNAc) receptor, viral attachment S1 proteins from GfCoV/2014 and GfCoV/2011 can bind to glycans capped with alpha-2,6-linked sialic acids. Our data indicate that diversity in GfCoV viral attachment proteins results in differences in degrees of affinity for glycan receptors, as well as altered avidity for intestinal tract tissues, which might have consequences for GfCoV tissue tropism and pathogenesis in guinea fowls. Glycan and tissue binding analyses of GfCoV/2011 and GfCoV/2014 recombinant spike S1 proteins revealed that while both proteins had the same specificities, GfCoV/2014 S1 had a much higher affinity toward glycan receptors and tissues of the lower gastrointestinal tract, in agreement with the observed replication of the virus in these tissues from field cases.	./cache/cord-325943-3hvy7b7c.txt	./txt/cord-325943-3hvy7b7c.txt