id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-322231-jltk42dt	Huy, Nguyen-Xuan	Immunogenicity of an S1D epitope from porcine epidemic diarrhea virus and cholera toxin B subunit fusion protein transiently expressed in infiltrated Nicotiana benthamiana leaves	2016-08-23		.txt	text/plain	6951	391	60	title: Immunogenicity of an S1D epitope from porcine epidemic diarrhea virus and cholera toxin B subunit fusion protein transiently expressed in infiltrated Nicotiana benthamiana leaves After oral administration of transiently expressed CTB–S1D fusion protein, or with bacterial cholera toxin or rice callus expressing mutant cholera toxin 61F, mice exhibited significantly greater serum IgG and sIgA levels against bacterial CTB and S1D antigen, peaking at week 6. For feeding immunization, the mice were fasted 8 h before gavage with 2.0 mL of PBS buffer pH 7.0 containing lyophilized leaf powder, which harbored 100 µg of CTB-S1D fusion protein, or with bacterial cholera toxin-bCT (Sigma) or rice callus-expressed mutant cholera toxin 61 F (Arg to Phe)-rCTX and wild-type (WT) N. Oral immunization with transient expression CTB-S1D fusion protein induced significantly higher anti-CTB, anti-S1D IgG and sIgA levels compared with the control group (Fig. 7 ).	./cache/cord-322231-jltk42dt.txt	./txt/cord-322231-jltk42dt.txt