id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-314751-i9rxesrg	Oh, Jongsuk	Immunogenicity and protective efficacy of recombinant S1 domain of the porcine epidemic diarrhea virus spike protein	2014-07-10		.txt	text/plain	6006	242	44	In this study, a codon-optimized PEDV S1 gene containing amino acid residues 25–738 was synthesized based on a multiple alignment of the S amino acid sequences of PEDV field isolates and used to establish a stable porcine cell line constitutively expressing the PEDV S1 protein. In the present study, therefore, we first synthesized a full-length, codon-optimized PEDV S1 gene and then generated a stable porcine-origin cell line constitutively expressing the recombinant S1 protein. These results were coincident with the rabbit study described above, since the rabbit antisera generated by immunization with the recombinant S1 protein also contained low levels of neutralizing antibodies to the heterologous PEDV vaccine strain. In the present study, the first aim was to stably express the full-length, codon-optimized S1 gene of PEDV in porcine-origin cells and to evaluate the immunogenicity and efficacy of the recombinant S1 protein.	./cache/cord-314751-i9rxesrg.txt	./txt/cord-314751-i9rxesrg.txt