id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-313988-3xjnpkqp	Ferraz, Rosa María	Insertional protein engineering for analytical molecular sensing	2006-04-03		.txt	text/plain	3516	147	25	In this context, insertional protein engineering offers the possibility to develop new devices, efficiently responding to ligand interaction by dramatic conformational changes, in which the specificity and magnitude of the sensing response can be adjusted up to a convenient level for specific analyte species. Among enzyme inhibitors and other few ligand species that activate allosteric biosensors, antibodies have been noted to be specially efficient allosteric effectors [36] and the use of antigenic peptides as receptors in only-protein biosensors would offer appealing tools for the fast molecular diagnosis of infectious diseases [39, 43] . For such a sensor being efficiently responsive, appropriated permissive sites need to be selected permitting proper receptor display and signal transduction, and the whole protein might require further engineering to gain specificity and response range. Among the diversity of sensing strategies based on insertional mutagenesis two protein platforms emerge as the most explored, namely cleavable sensors responding to proteases or their inhibitors, and allosteric, among whose most efficient effectors are antibodies.	./cache/cord-313988-3xjnpkqp.txt	./txt/cord-313988-3xjnpkqp.txt