id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-306624-1mjmttec	Wodrich, Harald	A Capsid-Encoded PPxY-Motif Facilitates Adenovirus Entry	2010-03-19		.txt	text/plain	11594	666	53	We show that the PPxY motif in protein VI is involved in its efficient microtubule-mediated transport and mutating it in the virus alters the intracellular targeting of Ads towards the MTOC region concomitant with a post-entry block in viral infectivity. To identify possible trafficking determinants, we analyzed the sequences of protein VI from several Ad serotypes and identified a highly conserved ubiquitin-ligase interacting motif present in PPxY-type viral late domains (PPxY, Figure S2 ). To determine whether the M1 mutation influences Ad cell entry, we performed a fluorescent focus forming assay and stained cells at 8, 12 and 24 h post-infection for expression of the E2A protein, which marks the appearance of viral replication centers ( Figure 2D and data not shown). In addition to the increase of protein VI capsid association, Ad5-VI-M1 appeared to be more evenly distributed throughout the cell and did not efficiently accumulate at the MTOC region ( Figure 3B , images to the left).	./cache/cord-306624-1mjmttec.txt	./txt/cord-306624-1mjmttec.txt