id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-306261-yc2y2xak	van Tricht, Ewoud	Fast, selective and quantitative protein profiling of adenovirus-vector based vaccines by ultra-performance liquid chromatography	2018-12-28		.txt	text/plain	5810	275	47	The new RP-UPLC method was successfully validated for protein profiling and relative quantification of proteins in vaccine products based on adenovirus vector types 26 and 35. By calculating the relative peak areas of all peaks in the chromatogram and identifying new peaks in the chromatogram, the method also allows the detections of degradation products in the sample Additional requirements for the method development were: a chromatographic run time of less than 30 min, baseline separation of all relevant adenovirus proteins allowing for their quantification, and an increased method robustness. The linear gradient with three slopes was replaced by a single-slope linear gradient to improve elution reproducibility and the flow rate was increased The critical method parameters of the RP-UPLC method were studied in a screening design of experiments to assess their impact on the method's run time and the resolution between the adenovirus proteins.: The following conditions were evaluated: gradient start composition (0-20% solvent B), gradient end composition (45-65% solvent B), gradient run time (17-25 min), TFA concentration (0.04-0.12%), and column temperature (40-70 • C).	./cache/cord-306261-yc2y2xak.txt	./txt/cord-306261-yc2y2xak.txt