id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-304040-64obh7i3	Sande, Charles J.	Untargeted analysis of the airway proteomes of children with respiratory infections using mass spectrometry based proteomics	2018-09-14		.txt	text/plain	5041	250	43	Our proteomic experiments tested the optimal approach to characterize airway proteome on swab samples eluted in UTM based on the number of proteins identified without BSA depletion (Total proteome: Protocol A) and with its depletion using a commercial kit; Allprep, Qiagen (cellular proteome: Protocol B, Ci, and Cii). Compared to previous mass-spectrometry-based proteomics studies of the upper airway, the number of proteins identified in protocol Cii, vastly exceeded the number of proteins reported in previously published reports 14, 20, 22, 23, 25, 26, 28, 29 . Further characterisation the proteomes identified in protocol Cii, was done by evaluating the expression levels of marker proteins of resident naso-oro-pharyngeal epithelial cells. In addition to these definitive upper-airway proteins, we found a consistently high level of expression of mucins (MUC1 & 5) -the main component of respiratory tract mucus -and mucosal-associated keratins, KRT4 and KRT13. Our search of T-cell associated marker and effector proteins, did not identify any such proteins in the upper-airway proteomes of any of the children in this study.	./cache/cord-304040-64obh7i3.txt	./txt/cord-304040-64obh7i3.txt