id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-302009-oqc21fah	Geng, Xindu	Protein folding liquid chromatography and its recent developments()	2007-04-15		.txt	text/plain	6669	334	49	Protein folding liquid chromatography (PFLC) is a new method developed in recent years, and it is widely used in molecular biology and biotechnology. When it is used in protein folding, the bioactivity recovery increases, the folded protein can be easily separated from misfolded forms, protein concentration after refolding is relatively high, and it is easy to scale up and automate, therefore it is regarded as an efficient, and close to ideal refolding method [1, 2] . In addition, by continuously changing the components of the mobile phase, different proteins can be separated with suitable folding conditions to refold and simultaneously purify in only one chromatographic run. Chaperone solvent plug SEC proposed by Liu and Chang [16] could obviously reduce precipitates formed before denatured protein entered the top of the column, and relatively high mass recovery was obtained. [57] recently proposed a relatively versatile refolding method using IEC with a silica-based weak cation exchanger, very high mass and bioactivity recoveries were obtained for denatured lysozyme.	./cache/cord-302009-oqc21fah.txt	./txt/cord-302009-oqc21fah.txt