id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-298759-j965t808	Jiang, Nan	Development of a robust Escherichia coli-based cell-free protein synthesis application platform	2020-10-17		.txt	text/plain	5253	298	55	The Escherichia coli-based system was chosen in this study, and the model protein deGFP was expressed to explore a more efficient cell-free system. To overcome these problems, cell-free protein synthesis (CFPS) has received new attention as an emerging synthetic biology technology, because it is an open system, not limited by cell growth or cell membrane, and it allows the use of various reaction formats. The selection of extracts, the size of the plasmid, the molecular crowding effect and the metal ion J o u r n a l P r e -p r o o f effect, which are important component parameters, were explored to improve the protein expression efficiency. Based on the optimized CFPS systems, the cell-free fundamental scientific research platform, primary screening platform, and portable biomolecular synthesis platform were established. Therefore, researchers need to optimize the metal ions and the corresponding concentration for the best cell-free protein synthesis.	./cache/cord-298759-j965t808.txt	./txt/cord-298759-j965t808.txt