id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-292958-k5d5fo3i	Sekhon, Simranjeet Singh	Porcine epidemic diarrhea (PED) infection, diagnosis and vaccination: A mini review	2017-01-04		.txt	text/plain	7430	299	41	Porcine epidemic diarrhea virus (PEDV) is a main etiology causing severe enteric disease in piglets with clinical signs of anorexia, vomiting, diarrhea and dehydration resulting in loss of condition and death within a few days. Many techniques are available for the detection of PEDV from fecal materials, small infected intestines sample, including reverse transcript polymerase chain reaction (RT-PCR), direct immunofluorescence tests (IF), indirect fluorescence antibody tests (IFA), immunohistochemistry techniques (IHC), in situ hydridization, electron microscopy and enzyme-linked immunosorbent assays (ELISA) 2 . (2012) used the Vero cell cultures to isolate CHGD-01 PEDV strain as well as employed direct immunofluorescence assay and EM technique to investigate its specific cytopathic effects in the outbreak of diarrhea in Guangdong (South China) swine 18 . The RT-PCR system in this study could effectively detect PEDV RNA from viral mixtures or small intestinal/ fecal samples in very low number of virus within short time.	./cache/cord-292958-k5d5fo3i.txt	./txt/cord-292958-k5d5fo3i.txt