id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-286301-7sjw5ci7	Sadasivan, Jibin	Cytoplasmic tail of coronavirus spike protein has intracellular targeting signals	2017-04-18		.txt	text/plain	6243	289	48	SARS-S-Y was absent from surface in most of the cells and localized at the intracellular compartments (figure 5a), and OC43-S protein was mainly localized in distinct puncta that could represent endocytic structures following internalization from the plasma membrane (figure 5b). Our studies clearly demonstrated that the KXHXX motif is the major intracellular localization signal of the full-length SARS-S protein and the C-terminal proximity is not essential. Our alanine mutation studies on the KXHXX motif confirm the importance of the lysine and histidine in the full-length wild-type HCoV-SARS S protein; the mutant protein showed localization in plasma membrane instead of the usual ER and ERGIC. In contrast, Lysosomal acid phosphatase, also a type I membrane protein with a cytosolic tail GYXXØ motif located 7 residues from both transmembrane domain and the carboxy termini (Tm-RMQAQPPGYRHVADGEDHA) delivered mainly via the cell surface (Braun et al.	./cache/cord-286301-7sjw5ci7.txt	./txt/cord-286301-7sjw5ci7.txt