id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-266481-9afb0yvt	Naskalska, Antonina	Membrane Protein of Human Coronavirus NL63 Is Responsible for Interaction with the Adhesion Receptor	2019-07-17		.txt	text/plain	5673	289	51	We have previously shown that the virus uses heparan sulfate proteoglycans (HSPGs) as the initial attachment factors, facilitating viral entry into the cell. However, we recently reported that heparan sulfate (HS) proteoglycans (HSPGs) are required for effective adhesion of the virus to the cell surface and that such an interaction enhances the infection process (12) . To further validate this result, we blocked the virus-ACE2 interaction with anti-ACE2 antibody, which resulted in inhibition of MENS VLP internalization but not adhesion to the cell surface (Fig. 3) . Using flow cytometry, we next investigated whether the adhesion of MEN and MENS VLPs to the cell surface involves interaction with HSPGs, as has been shown for HCoV-NL63. Mapping of the receptor-binding domain and amino acids critical for attachment in the spike protein of avian coronavirus infectious bronchitis virus	./cache/cord-266481-9afb0yvt.txt	./txt/cord-266481-9afb0yvt.txt