id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-261375-6fu3dzi9	Hoppe, Sebastian	Microarray-based method for screening of immunogenic proteins from bacteria	2012-03-21		.txt	text/plain	6693	389	51	Screening of expression libraries to expose novel antigens from bacteria often lead to an abundance of false positive signals owing to the high cross reactivity of polyclonal antibodies towards the proteins of the expression host. In this paper, we describe a method to covalently attach different HaloTag ® fusion proteins on HaloLink™ slides (see Figure 1 ) and consequently perform an immunoscreening using polyclonal antibodies in a microarray format, which is a suitable method for high-throughput applications such as screening of entire expression libraries (see Figure 2 ). Using our new method, we were able to express, immobilize and screen all of nine different proteins from Campylobacter jejuni using HaloTag ® and KRX cells. We were able to clone several genes from Campylobacter jejuni into KRX cells, to express the respective proteins as fusion constructs with a HaloTag ® attached to their N-Terminus and to immobilize these proteins on microarray surfaces.	./cache/cord-261375-6fu3dzi9.txt	./txt/cord-261375-6fu3dzi9.txt