id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-261159-9pkg7mbh	Regnier, Fred E.	Chromatography of complex protein mixtures	1987-07-17		.txt	text/plain	10934	565	51	Although large amounts of organic solvent are occasionally used in the mobile phase to solubilize proteins, as in the case of chloroplast proteins [ 181, most IEC separations are carried out with either non-ionic detergents or the 3-[ ( 3-chloroamidopropyl) dimethylammonio] -l-trifluoroacetic acid ( CHAPS) zwitterion [ 24,251. Using a series of polyethylene glycol alkyl ether (C,E,) detergents as a mobile phase additive it was possible to show that both alkyl chain length and the number of glycol residues influenced resolution of Escherichiu coZi K-12 membrane proteins in anion-exchange separations [ 191 (C&E, was optimal) . Elution of proteins and peptide fragments from RPC columns was achieved with mobile phases containing 5% formic acid. In the case of red blood cell membrane proteins, the resolution of 1000 A pore diameter organic resin-based sorbents was superior to 300 A pore diameter silica-based materials when the columns were eluted with a TFA-acetonitrile mobile phase [ 261.	./cache/cord-261159-9pkg7mbh.txt	./txt/cord-261159-9pkg7mbh.txt