id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-256340-w4z5avld	Bailer, SM	Connecting viral with cellular interactomes	2009-07-24		.txt	text/plain	3649	167	38	Genome-scale screens for intraviral and virus–host protein interactions and the analysis of literature-curated datasets are able to provide a novel, comprehensive perspective of viruses, and virus-infected cells. Until now, large-scale interaction screens were predominantly performed with the yeast-two-hybrid (Y2H) system; however, alternative high-throughput technologies detecting binary protein interactions or protein complexes have been developed. The Y2H system as the standard assay for the evaluation of interactomes Essentially all high-throughput approaches to identify binary protein interactions on a genome-scale currently rely on the Gal4-based yeast-two-hybrid (Y2H) system ( Figure 1 ) developed in 1989 [1] . Despite certain limitations the Y2H system is used by the majority of groups because of its enormous efficacy and the data discussed in this review are all based on Y2H screens as all currently published large-scale studies on intraviral or virus-host protein interactions are based on them. More recent approaches on intraviral interactomes include several members of the herpesvirus family [15 ,16 ,19 Scheme of HSV-1 virus particle with protein interactions detected in a genome-wide Y2H screen.	./cache/cord-256340-w4z5avld.txt	./txt/cord-256340-w4z5avld.txt