id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-104279-choywmwd	nan	Membrane protein sorting in the yeast secretory pathway: evidence that the vacuole may be the default compartment	1992-10-01		.txt	text/plain	9856	411	52	First, to determine if the lumenal domain was sorted to the vacuole when expressed in the secretory pathway as a soluble protein, a gene fusion was used to create the protein ctFss-B, consisting of the NH2-terminal ER-targeting signal sequence of prepro-t~ factor fused to the lumenal domain of DPAP B at residue 49 ( Fig. 1 B) . To ad-dress the possibility that the mutant constructs analyzed in this study were mislocalized to the plasma membrane followed by rapid endocytic uptake to the vacuole, indirect immunofluorescence experiments were performed on BB-Inv, B-A-B, A20-BB, and/x22-AA-B expressed in a secl strain at 34~ As a positive control for accumulation in secretory vesicles, the localization of the fusion protein Fusl-LacZp was analyzed (Trueheart et al., 1987) .	./cache/cord-104279-choywmwd.txt	./txt/cord-104279-choywmwd.txt