id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-018479-mvnm98hv	Rehm, Fabian B. H.	Applications of Microbial Biopolymers in Display Technology	2017-11-16		.txt	text/plain	4621	227	35	Such beads have been demonstrated in diverse applications, including fluorescence-activated cell sorting, enzyme-linked immunosorbent assays, microarrays, diagnostic skin test for tuberculosis, vaccines, protein purification, and affinity bioseparation. Most recently, PHA inclusions with GFP and MOG fused to PhaP or PhaC, such that each granule is simultaneously displaying two protein-based functionalities, were examined in the context of FACS, providing proof-of-concept for the biotechnological application of bifunctional PHA beads which may extend beyond FACS ). This study demonstrated that V HH domains from camelid antibodies, designed ankyrin repeat proteins (DARPins) and OB-folds (OBodies), could be densely displayed on PHA beads resulting in high affinity binding resins for purification of various target proteins. Overall, PhaC engineering such as N-or C-terminal fusions and/or insertions enabled efficient display of binding domains for interaction with target compounds resulting in purification performance suitable for application as bioseparation resin.	./cache/cord-018479-mvnm98hv.txt	./txt/cord-018479-mvnm98hv.txt