id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-002739-7t1o19kn	Yu, Xiaobo	Multiplexed Nucleic Acid Programmable Protein Arrays	2017-09-20		.txt	text/plain	6451	296	49	Methods: In this work, we developed the Multiplexed Nucleic Acid Programmable Protein Array (M-NAPPA), which significantly increases the number of displayed proteins by multiplexing as many as five different gene plasmids within a printed spot. Protein-protein interactions and serological antibody assays using human viral proteome microarrays demonstrated that comparable hits were detected by M-NAPPA and non-multiplexed NAPPA arrays. We developed the Multiplexed Nucleic Acid Programmable Protein Array (M-NAPPA) method by combining as many as five different DNA plasmids within one spot, which increases the number of displayed proteins per microarray by five-fold. To decrease the cost of high throughput screening experiments, we hypothesized that the plasmid cDNA encoding for different proteins could be multiplexed (by combining M different plasmids) within each feature to create a high-density array, M-NAPPA ( Figure 1A) . Since the multiplex concept to increase feature density was successful in detecting protein-protein interactions and serological antibody responses on planar microarrays, we wanted to determine whether M-NAPPA could also be applied to a nano-well microarray platform.	./cache/cord-002739-7t1o19kn.txt	./txt/cord-002739-7t1o19kn.txt