id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-305472-w33k8pdu	Ojosnegros, Samuel	Topology of evolving, mutagenized viral populations: quasispecies expansion, compression, and operation of negative selection	2008-07-17		.txt	text/plain	8024	411	42	Here we apply for the first time phylogenetic methods and Partition Analysis of Quasispecies (PAQ) to monitor genetic distances and intra-population structures of mutant spectra of foot-and-mouth disease virus (FMDV) quasispecies subjected to mutagenesis by base and nucleoside analogues. In the course of the studies on lethal mutagenesis of FMDV, biological clones of the virus were subjected to serial cytolytic passages in cell culture in the absence or presence of the mutagenic bases or nucleoside analogues 5-fluorouracil (FU), ribavirin (1-β-D ribofuranosyl-1, 2, 3-triazole-3 carboxamide) (R), or 5-azacytidine (AZC) [32, [38] [39] [40] [41] . Comparison of the phylogenetic trees derived from nucleotide sequences of the different FMDV clonal populations passaged 1 and 25 times in the absence or the presence of AZC or FU shows an expansion of genetic distances among components of the mutant spectrum of the mutated populations compared to the respective control populations passaged in the absence of drug.	./cache/cord-305472-w33k8pdu.txt	./txt/cord-305472-w33k8pdu.txt