id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-258927-masvn1gu	Soria-Guerra, Ruth Elena	Expression of a multi-epitope DPT fusion protein in transplastomic tobacco plants retains both antigenicity and immunogenicity of all three components of the functional oligomer	2009-03-21		.txt	text/plain	5740	301	50	title: Expression of a multi-epitope DPT fusion protein in transplastomic tobacco plants retains both antigenicity and immunogenicity of all three components of the functional oligomer We report the introduction and expression of a fusion DPT protein containing immunoprotective exotoxin epitopes of Corynebacterium diphtheriae, Bordetella pertussis, and Clostridium tetani in tobacco chloroplasts. Recently in our laboratory, a fusion protein of the heat labile toxin B subunit (LTB) of the enterotoxigenic Escherichia coli along with the heat stable toxin (ST) fusion protein (LTB-ST) has been expressed in transplastomic tobacco plants, and demonstrated its immunogenic characteristic in tested mice (Rosales-Mendoza et al. Recently, a novel polypeptide containing the DPT immunoprotective exotoxin epitopes has been designed and used to demonstrate expression of this fusion protein in transgenic tomato plants ). Western blots and ELISA assays conWrmed that the tobacco-derived DPT protein was recognized by speciWc antibodies against each of diphtheria, pertussis, and tetanus toxins.	./cache/cord-258927-masvn1gu.txt	./txt/cord-258927-masvn1gu.txt