id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-005205-z6bx0309	Herbers, Karin	A simplified procedure for the subtractive cDNA cloning of photoassimilate-responding genes: isolation of cDNAs encoding a new class of pathogenesis-related proteins	1995		.txt	text/plain	5212	289	53	Transgenic tobacco plants (ppa-1) constitutively expressing Escherichia coli pyrophosphatase behind the 35S CaMV promoter accumulate high levels of soluble sugars in their leaves [27]. One of these clones (termed PAR-1 for photoassimilate-responsive) displayed features similar to pathogenesis-related proteins: Hybridizing transcripts, 1.2 and 1.0 kb in length, were strongly inducible by salicylate and accumulated in tobacco plants after infection with potato virus Y (PVY) both in infected and uninfected systemic leaves. As cDNAs coding for PR-proteins had been isolated from the ppa-l-specific library supporting the hypothesis that sugar accumulation in leaves would result in a general stress response we wondered whether PAR-1 also possessed any features of stress-related genes. To discriminate between the differently sized PAR-1 transcripts DNA fragments were isolated from the putative 3'-untranslated regions of the three PAR-1 cDNAs. These were used as probes in northern blots to analyse the accumulation of PAR-1 mRNAs in infected and uninfected leaves of PVY-treated tobacco plants.	./cache/cord-005205-z6bx0309.txt	./txt/cord-005205-z6bx0309.txt