id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-005145-1l87fdmi	Marquet-Blouin, E.	Neutralizing immunogenicity of transgenic carrot (Daucus carota L.)-derived measles virus hemagglutinin	2003		.txt	text/plain	5640	282	49	Despite differences in post-translational processing viral and bacterial antigens preserved their immunogenic properties when produced in plants and induced cross-reactive and sometimes neutralizing and protective antibodies. The aim of this study was (1) to explore the potential of carrots as an expression system for antigens that is suitable for human consumption, and (2) to test whether the measles virus hemagglutinin glycoprotein would preserve its neutralizing immunogenicity in this system. Although some work has been done with transgenic carrot callus cells (Brodzik et al., 2000) , this is one of the first reports of the expression of a transgenic antigen in mature carrots, showing that high levels of virus-neutralizing antibodies can be induced with a glycoprotein produced in this plant. The flow cytometry data showed that all mice vaccinated with transgenic leaf or root extracts produced high levels of antibodies cross-reacting with the native protein independently whether virus-infected or H-protein transfected cells were used. Plant-derived measles virus hemagglutinin protein induces neutralizing antibodies in mice	./cache/cord-005145-1l87fdmi.txt	./txt/cord-005145-1l87fdmi.txt