id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-310844-7i92mk4x	Hryhorowicz, Magdalena	Application of Genetically Engineered Pigs in Biomedical Research	2020-06-19		.txt	text/plain	9011	475	37	Animal studies are conducted to develop models used in gene function and regulation research and the genetic determinants of certain human diseases. Short pregnancy, short generation interval, and high litter size make the production of transgenic pigs less time-consuming in comparison with other livestock species This review describes genetically modified pigs used for biomedical research and the future challenges and perspectives for the use of the swine animal models. It was demonstrated that precise integration of the human CFTR gene at a porcine safe harbor locus through CRISPR/Cas9-induced HDR-mediated knock-in allowed the achievement of persistent in vitro expression of the transgene in transduced cells. The study showed that multiple genetically modified porcine hearts were protected from complement activation and myocardial natural killer cell infiltration in an ex vivo perfusion model with human blood [86] . Biomedical applications for which genetically engineered pigs are generated include modeling human diseases, production of pharmaceutical proteins, and xenotransplantation.	./cache/cord-310844-7i92mk4x.txt	./txt/cord-310844-7i92mk4x.txt