id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-277716-6gsmkmk5	Doll, Tais A. P. F.	Design and optimization of peptide nanoparticles	2015-10-24		.txt	text/plain	5892	309	55	RESULTS: Here, we were studying in vitro and in silico the effect of the chain length and of point mutations near the linker region between the pentamer and the trimer on the self-assembly of the SAPNs. 60 identical peptide chains co-assemble to form a spherical nanoparticle displaying icosahedral symmetry. All these different mutants along with the initial parent peptide (Fig. 3) were studied by molecular dynamics (MD) simulations using the program CHARMM 36b1 in an attempt to further optimize the linker region and find the best peptide building block for the SAPNs. From the linker constitution study it was found that a malaria nanoparticle vaccine construct self-assembled into almost spherical nanoparticles [21] . Based on our construct design and the biophysical results of the 2.5HR we ran a series of molecular dynamics simulations on the eleven short versions of 2.5HR including only five helical turns (i.e. 2.5 heptad repeats) around the linker region of the nanoparticle peptide (Figs.	./cache/cord-277716-6gsmkmk5.txt	./txt/cord-277716-6gsmkmk5.txt