id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-269462-7yozebv2	Vitiello, Mariateresa	Viral Fusion Peptides Induce Several Signal Transduction Pathway Activations That Are Essential for Interleukin-10 and Beta-Interferon Production	2010-07-02		.txt	text/plain	5195	229	47	METHODS: Fusion peptides of several enveloped viruses belonging to different virus families were prepared by standard 9-fluorenylmethoxycarbonyl polyamine solid-phase synthesis and used to stimulate U937 cells in vitro to analyze the phosphorylation patterns of the signaling pathways (PKC, Src, Akt, and MAPK pathways). Specific ligand-receptor interactions result in activation of signaling pathways; thus, we selected fusion peptides belonging to each of the known viral classes of fusion glycoproteins in order to verify whether during the interaction with the cell surface, they were sufficient to induce phosphorylation of PKC, Src, Akt, and MAPK pathways, activation of nuclear factor (AP-1 and NF-B), and cytokine release (IL-10 and IFN-␤ ). To selectively analyze the regulation of virus-induced AP-1 and NF-B activation, an ELISA-based Trans-Am technology from nuclear lysates of U937 cells stimulated by viral fusion peptides was performed.	./cache/cord-269462-7yozebv2.txt	./txt/cord-269462-7yozebv2.txt