id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-009492-lhwhkada	Kašička, Václav	Recent developments in CE and CEC of peptides	2007-11-28		.txt	text/plain	17890	665	36	Novel developments in individual CE and CEC modes are shown and several types of their applications to peptide analysis are presented: conventional qualitative and quantitative analysis, purity control, determination in biomatrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid and sequence analysis, and peptide mapping of proteins. Preconcentration and preseparation (or "sample cleanup") are the necessary operations for sample preparations, when separation power and/or sensitivity of CE and CEC are not sufficient for direct analysis of peptides present at low concentration levels and/or in complex mixtures of different (bio)matrices, such as body fluids, cell lysates, and tissue extracts. After optimization the injection conditions, such as type and concentration of the acid and organic solvent in the sample solution, length of the water plug, sample injection time, and separation voltage, more than 3000-fold improvement in detection signal was obtained, permitting analysis of bioactive peptides and tryptic protein digests in low nanomolar (fmol/mL) levels.	./cache/cord-009492-lhwhkada.txt	./txt/cord-009492-lhwhkada.txt