id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-331542-wy068c6o	Kong, Ning	Identification of a novel B-cell epitope in the spike protein of porcine epidemic diarrhea virus	2020-04-03		.txt	text/plain	3691	198	53	The antisera titers were determined by indirect ELISA, western blot and IFA after four immunizations to find the important immunodominant region of S1, and then purified the immunodominant region of S1 protein and immunized mice to generate the special antibodies, and then used recombinant peptides to determine the B-cell epitopes of monoclonal antibodies. To accurately identify the immunodominant region of S1, the purified truncated S1 proteins (SA, SB, SC, SD and SE) were used to immunize BALB/c mice to prepare Fig. 1 Detection of recombinant proteins' antigenicity for polyclonal antisera (PcAbs) by indirect ELISA, western blot and IFA. The indirect ELISA and western blot analysis showed that the SE polyclonal antisera had the highest antibody titers against PEDV S1 protein ( Fig. 1a and b) , suggesting that SE protein was the important immunodominant region of S1.	./cache/cord-331542-wy068c6o.txt	./txt/cord-331542-wy068c6o.txt