id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-331509-p19dg1jw	Bigault, Lionel	Porcine epidemic diarrhea virus: Viral RNA detection and quantification using a validated one-step real time RT-PCR	2020-05-31		.txt	text/plain	4317	240	60	title: Porcine epidemic diarrhea virus: Viral RNA detection and quantification using a validated one-step real time RT-PCR In this study we described the development and validation of a SYBR™ Green one-step RT-qPCR according to the French norm NF U47-600, for the detection and quantification of PEDV viral RNA. Specificity and sensitivity, limit of detection (LoD), limit of quantification (LQ), linearity, intra and inter assay variability were evaluated using transcribed RNA and fecal and jejunum matrices spiked with virus. For a rapid, accurate and reliable diagnosis of PED in the veterinary laboratory, a method for the detection of PEDV viral RNA has been developed and more importantly validated according to the "Association Francaise de NORmalisation" (AFNOR) French NF U47-600 norm entitled "requirement and recommendation for the implementation, development and validation of PCR in animal health" (AFNOR, 2015a; AFNOR, 2015b) . This method should help harmonize detection and quantification of viral RNA from PEDV belonging to both S-non-INDEL and S-INDEL strains in both field and experimental settings.	./cache/cord-331509-p19dg1jw.txt	./txt/cord-331509-p19dg1jw.txt