id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-330035-0d6w8xyd	Jeon, Ji Hyun	Cellular cholesterol is required for porcine nidovirus infection	2017-09-07		.txt	text/plain	7673	328	41	The addition of exogenous cholesterol to MβCD-treated cells moderately restored the infectivity of porcine nidoviruses, indicating that the presence of cholesterol in the target cell membrane is critical for viral replication. The addition of exogenous cholesterol to MβCD-treated cells moderately restored the infectivity of porcine nidoviruses, indicating that the presence of cholesterol in the target cell membrane is critical for viral replication. Furthermore, pharmacological sequestration of cellular cholesterol efficiently blocked both virus attachment and internalization and, accordingly, markedly affected subsequent post-entry steps of the replication cycle, including viral RNA and protein biosynthesis and progeny virus production. Further experiments revealed that pharmacological depletion of cellular cholesterol primarily interferes with virus binding and penetration and subsequently influences post-entry stages of the PRRSV and PEDV replication cycle, including viral genomic and sg RNA synthesis, viral protein expression, and virus production.	./cache/cord-330035-0d6w8xyd.txt	./txt/cord-330035-0d6w8xyd.txt