id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-321814-vt6yio6x	Pan, Yongfei	Isolation and characterization of a variant porcine epidemic diarrhea virus in China	2012-09-12		.txt	text/plain	3657	187	51	In this study, we isolated a PEDV strain designated CHGD-01 from piglet intestines using Vero cell cultures, and its specific cytopathic effects were confirmed in susceptible cells by direct immunofluorescence testing and electron microscopy. Amino acid sequence analysis based on individual virus genes indicated a close relationship between the spike protein gene of CHGD-01 and the field strain KNU0802 in Korea. Phylogenetic analyses of the S protein amino acid sequences revealed that all PEDV strains in this study could be separated into two groups: CHGD-01 belonged to Group 2, which also contained the two Japanese isolates Kawahira and NK, eight Korean field strains (Chinju99, KNU-0801, KNU-0802, and KNU-0901-KNU-0905) and two Chinese strains (BJ-2011-1 and CH/FJND-3/2011), which were deposited in GenBank in 2011 ( Figure 3b ). Molecular characterization and phylogenetic analysis of membrane protein genes of porcine epidemic diarrhea virus isolates in China	./cache/cord-321814-vt6yio6x.txt	./txt/cord-321814-vt6yio6x.txt