id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-301655-6nxhvvm4	Lei, Xi-Mei	Specific recombinant proteins of porcine epidemic diarrhea virus are immunogenic, revealing their potential use as diagnostic markers	2019-08-10		.txt	text/plain	6431	284	49	In order to determine and compare which of the viral proteins may be useful as diagnostic antigens, whole virus (WV) particles and a panel of structural and nonstructural PEDV proteins [spike subunit 1 (S1), the C-terminal part of ORF3 (ORF3C), envelope (E), nonstructural protein 1 (Nsp1), Nsp2, Ac (acidic domain of Nsp3), and ADRP (ADP-ribose-1-monophosphatase domain of Nsp3), expressed individually in bacterial and/or mammalian cells] were tested for reactivity with sera from PEDV-infected pigs by ELISA and/or western blot analysis. In this study, a panel of recombinant PEDV ORFs encoding structural and nonstructural proteins were expressed in mammalian and/or bacterial cells and screened for reactivity with porcine sera from seven provinces of China by ELISA and/or western blot analysis, in order to determine which antigen is most suitable as a diagnostic marker for PEDV infection.	./cache/cord-301655-6nxhvvm4.txt	./txt/cord-301655-6nxhvvm4.txt