id author title date pages extension mime words sentences flesch summary cache txt cord-301355-9lswjro2 Fan, Jing-Hui Development of an enzyme-linked immunosorbent assay for the monitoring and surveillance of antibodies to porcine epidemic diarrhea virus based on a recombinant membrane protein 2015-12-01 .txt text/plain 3974 208 52 title: Development of an enzyme-linked immunosorbent assay for the monitoring and surveillance of antibodies to porcine epidemic diarrhea virus based on a recombinant membrane protein Therefore, the accurate diagnosis, serological monitoring, and surveillance of specific antibodies in pigs resulting from porcine epidemic diarrhea virus (PEDV) infection or vaccination would be essential in helping to control the spread of PED. To detect PEDV antibodies in eight herds, 382 serum samples were collected from sows that had been immunized with a PED vaccine, and screened using the developed ELISA in parallel with a serum neutralization (SN) assay. To determine the specificity of the developed ELISA, crossreactivity was assessed by determining the reactivity of the purified antigen with serum samples containing antibodies against TGEV, PRV, PRRSV, PCV2, CSFV and PEDV. Development and evaluation of enzyme-linked immunosorbent assay based on recombinant nucleocapsid protein for detection of porcine epidemic diarrhea (PEDV) antibodies ./cache/cord-301355-9lswjro2.txt ./txt/cord-301355-9lswjro2.txt