id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-261372-xjbs09gi	Sozzi, Enrica	Comparison of enzyme-linked immunosorbent assay and RT-PCR for the detection of porcine epidemic diarrhoea virus	2010-02-28		.txt	text/plain	2092	94	51	A double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) based on the use of monoclonal antibodies was developed for the detection of porcine epidemic diarrhoea virus (PEDV). The objective of the present study was to develop a double antibody sandwich (DAS) ELISA, based on the use of monoclonal antibodies for PEDV detection in swine intestinal and faecal samples useful for routine examinations of field samples. It is already known that ELISA results depend on the clinical and pathological data: in intestinal contents and faecal specimens obtained from experimentally infected PEDV piglets, the virus shedding is detected with high consistency during the acute phase of disease, but much less frequently during the incubation period and the recovery phase (Callebaut et al., 1982) . Enzyme-linked immunosorbent assay for the detection of the coronavirus-like agent and its antibodies in pigs with porcine epidemic diarrhea	./cache/cord-261372-xjbs09gi.txt	./txt/cord-261372-xjbs09gi.txt