id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-261036-zdhg4axx	Shirato, Kazuya	Enhanced cell fusion activity in porcine epidemic diarrhea virus adapted to suckling mice	2010-09-09		.txt	text/plain	3330	193	62	PEDV obtained after ten passages through the brains (MK-p10) had increased virulence for mice, and its fusion activity in cultured cells exceeded that of the original strain. One of these (an H-to-R substitution at residue 1,381) was first detected in PEDV isolated after eight passages, and both this virus (MK-p8) and MK-p10 showed enhanced syncytium formation relative to the original MK strain and viruses isolated after two, four, and six passages, suggesting the possibility that the H-to-R mutation was responsible for this activity. Expression of the receptor protein for mouse hepatitis virus (MHV) and severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) in cultured cells confers susceptibility to each of these viruses [2, 4, 25] . In the present study, we isolated a strain of PEDV that was more virulent than the original tissue-culture-adapted virus after passage through mouse brain cells. To investigate viral virulence and replication in mouse brains, 0-day-old mice were infected i.c. with 5,000 PFU of PEDV and monitored daily for clinical features; they were also weighed daily.	./cache/cord-261036-zdhg4axx.txt	./txt/cord-261036-zdhg4axx.txt